LOCUS       NZ_CP053239             7618 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-106 plasmid pSCU-106-5, complete
            sequence.
ACCESSION   NZ_CP053239
VERSION     NZ_CP053239.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609771
            Assembly: GCF_013085185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7618)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:10:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,452
            CDSs (total)                      :: 5,328
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 108 of 269
            Pseudo Genes (incomplete)         :: 154 of 269
            Pseudo Genes (internal stop)      :: 52 of 269
            Pseudo Genes (multiple problems)  :: 47 of 269
            Pseudo Genes (short protein)      :: 3 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053239.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-106"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-106-5"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            complement(<274..>429)
                     /locus_tag="HHJ24_RS26815"
                     /old_locus_tag="HHJ24_26805"
                     /pseudo=""
     CDS             complement(<274..>429)
                     /locus_tag="HHJ24_RS26815"
                     /old_locus_tag="HHJ24_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245253.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]; GO:0020037 - heme binding [Evidence IEA]"
                     /GO_process="GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /name="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(<442..786)
                     /locus_tag="HHJ24_RS26820"
                     /old_locus_tag="HHJ24_26810"
                     /pseudo=""
     CDS             complement(<442..786)
                     /locus_tag="HHJ24_RS26820"
                     /old_locus_tag="HHJ24_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310991.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(832..2172)
                     /locus_tag="HHJ24_RS26825"
                     /old_locus_tag="HHJ24_26815"
     CDS             complement(832..2172)
                     /locus_tag="HHJ24_RS26825"
                     /old_locus_tag="HHJ24_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_038989331.1"
                     /translation="MSILQFLIAVDVTVVNVALPSIGNDLGVDARGLTWVVVGYTITGG
                     GLLMLGGRLGDVFGRRRLLLIGTAIFGAASLMAGLAETFPLLVGARLLQGVGEALALPA
                     AMATIVLLFPEGRARTRALSVWAAVASCGLVLGFVLSGIITEYLGWRWIFLIAVPFVLF
                     VLIASVALLPRDRSQERAPLDILGSILLTSAPLLFAFGVVEAGDSNGDVPWSSIAALVG
                     AAAAAALFVKVERRAANPLVPLTFFRNRTRVRANLATALLSAALSTSFLLFTFYLQDGL
                     GLSPLESGLLLLPLAVALVVAVTVVPRLLGRWGARACIVVGLSSAGAGMIVIAAAAQLE
                     APAWLLTPAMLFIAAGMGFGLVGLQYAAVTEVTDDDAGVASGVQRAADQLGGSAGVTVY
                     VGLGFASALHGVSPYVLSSGLAIIGLCLATAIVQRIVIVTQEQQTRT"
                     /name="MFS transporter"
     gene            complement(2554..3405)
                     /gene="repC"
                     /locus_tag="HHJ24_RS26830"
                     /old_locus_tag="HHJ24_26825"
     CDS             complement(2554..3405)
                     /gene="repC"
                     /locus_tag="HHJ24_RS26830"
                     /old_locus_tag="HHJ24_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(3392..4231)
                     /locus_tag="HHJ24_RS26835"
                     /old_locus_tag="HHJ24_26830"
     CDS             complement(3392..4231)
                     /locus_tag="HHJ24_RS26835"
                     /old_locus_tag="HHJ24_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(4261..4467)
                     /gene="urfF"
                     /locus_tag="HHJ24_RS26840"
                     /old_locus_tag="HHJ24_26835"
     CDS             complement(4261..4467)
                     /gene="urfF"
                     /locus_tag="HHJ24_RS26840"
                     /old_locus_tag="HHJ24_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(4469..4681)
                     /locus_tag="HHJ24_RS26845"
                     /old_locus_tag="HHJ24_26840"
     CDS             complement(4469..4681)
                     /locus_tag="HHJ24_RS26845"
                     /old_locus_tag="HHJ24_26840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(4742..5665)
                     /locus_tag="HHJ24_RS27585"
     CDS             complement(4742..5665)
                     /locus_tag="HHJ24_RS27585"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_014344452.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(5710..6123)
                     /locus_tag="HHJ24_RS27590"
     CDS             complement(5710..6123)
                     /locus_tag="HHJ24_RS27590"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(<6233..6766)
                     /locus_tag="HHJ24_RS27595"
                     /pseudo=""
     CDS             complement(<6233..6766)
                     /locus_tag="HHJ24_RS27595"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            7070..7354
                     /locus_tag="HHJ24_RS26855"
                     /old_locus_tag="HHJ24_26850"
     CDS             7070..7354
                     /locus_tag="HHJ24_RS26855"
                     /old_locus_tag="HHJ24_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     Typing          complement(6961..7116)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(3393..4214)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(4746..6871)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcctagtgg attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc
       61 cgcccctgct gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt
      121 tattgcaggg gggcgtgaca gttattgcag gggggcgtga cagttattgc aggggttcgt
      181 gacagttagt acgggagtga cgggcactgg ctggcgttga gcagatcgtt accgacagtt
      241 cagcgtcgcg gcagttacgc ctcgtgcagt ggcgggaaac gccagatccc gggtgccgag
      301 cattttgggt atgagataca tcgccagacc ttcgagcacc ggaatcgcaa ataaaaacat
      361 catcaccgtg ccgtgcaggg taaagacctg gttgtacaca tcggcacgga gaatgtcctg
      421 tcccggcaag ggggtatatt agaactcttg cgcgtcgagt cgatcttcga ggtaggcggc
      481 gtactgccag cgcacctggt cgagcatgta gtcggtgtcg agatagatcg tggtgacggt
      541 gatgtggccc tctggctccg atcctgccag tacgttcgca ccgagcaaga tcacgtctcc
      601 gggtttcacc gtctgctgtc caaactcgct gaacaagaac gcagagccgt cacgcaccac
      661 gatcaccttg acgcagtcat acgccaccgg accgagaggg cggtgaatgg tcgcggtgcg
      721 cgcaacaaag ggcgcgcccc gcgaccaccg gtcggggctg actgacgagt cgccgggcgt
      781 cgccacggag cacctcatcg ggaacttcgg gatcgggtta cgacggcacg ctcatgtcct
      841 cgtctgttgt tcctgcgtca cgattacgat tcgttggaca atggcggtcg caaggcagag
      901 gccgatgatc gcgaggccgc tacttagcac atagggtgat acaccgtgaa gggccgacgc
      961 gaagcccagg cccacgtaga ccgtgacacc tgcggatcca ccgagctggt ctgcggctcg
     1021 ctgtacgccg gatgcgactc cggcatcatc gtcggtgacc tcggtcacgg ccgcgtactg
     1081 caatccaacg aggccgaaac ccattcctgc ggcgatgaag agcatcgcgg gggttagtag
     1141 ccaagcgggg gcttcgagct gcgcggctgc cgcgatcacg atcatgcctg cccctgcgga
     1201 actcagtccc acgacgatgc aagcacgcgc tccccagcgc cccaagaggc gaggtacgac
     1261 ggtcacggcg acgactagtg ccaccgcaag tggcaggagc agcaatccgg attccagcgg
     1321 agagagaccg agtccatcct gtagatagaa cgtgaacagc agaaaggacg ttgagagcgc
     1381 cgcgctcaac agtgcagtcg caagatttgc tcgcactcgt gtccggttgc ggaagaaggt
     1441 cagcggaacc agtgggtttg cggctcggcg ttccaccttg acgaacaatg cagcggcggc
     1501 tgccgctccc accagggcag cgattgatga ccagggcaca tccccattgc tgtccccggc
     1561 ctccaccact ccgaaagcga acagcagcgg tgcgctcgtc agcagaatcg atccgagaat
     1621 atccaacggt gcgcgctcct gtgatcggtc tcgaggtagc agggcgacag aagcgatcag
     1681 cacgaacagg acgaacggaa cggcgatgag gaagatccat cgccaaccga gatactcggt
     1741 gatgattccc gagagcacga accccagcac aagaccgcag ctcgcaaccg cagcccagac
     1801 gctcagggcg cgggtgcgcg cccgcccttc gggaaacaac aggacgatgg tcgccatcgc
     1861 agcgggcagg gcgagggctt cccctacacc ctgcaacaac cttgcaccga cgagtagcgg
     1921 aaaggtctcc gcgaggcccg ccatcagcga ggccgctccg aagatcgcgg ttccgatgag
     1981 cagaaggcgg cggcggccga agacgtctcc cagacgtcca ccaagcatca acagaccacc
     2041 gcctgtgatg gtgtaaccaa cgacgaccca tgtcaggccg cgcgcatcaa cgcccagatc
     2101 gttgccgata gagggcagcg ccacgttgac cacggtgacg tcgacggcga tgaggaattg
     2161 caggatggac agacaaacaa ggactgccca ggaacgagcg accgatactt gcgcaggccg
     2221 cgatgaagag gtgctaaaag acacagagaa ctccgttgct cgaatgaaga tcgggcatga
     2281 gtattatacg gatgctcggg tcgaaaggga atccccaggc gagtaacctg tttgcggtga
     2341 tccattagct gcaggagcag aagagcatac atctggaagc aaagccagga aagcggccta
     2401 tggagctgtg cggcagcgct cagtaggcaa tttttcaaaa tattgttaag ccttttctga
     2461 gcatggtatt tttcatggta ttaccaatta gcaggaaaat aagccattga atataaaaga
     2521 taaaaatgtc ttgtttacaa tagagtgggg gggtcagcct gccgccttgg gccgggtgat
     2581 gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca gctccggcaa
     2641 cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct ctgacggcca
     2701 gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt cgatccagcc
     2761 acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat
     2821 gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg ggttcagggc
     2881 cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg
     2941 ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga tgcagtcctg
     3001 tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg ccagcgcccg
     3061 atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg gttccaggaa
     3121 cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta ggccattagg
     3181 cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca gcggctccgg
     3241 gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg
     3301 cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt gcgccgggtc
     3361 gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc acccccttgc
     3421 tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc cgcctgaacc
     3481 accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg acgaagaacc
     3541 ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg ctcgacaggt
     3601 aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc tgctgctggt
     3661 cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg atagagcacc
     3721 cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg ccgctggcgt
     3781 tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg cggccctcgg
     3841 cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg ctgccgatca
     3901 gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc
     3961 caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca
     4021 ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca atctgtgcgg
     4081 ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc gccccgaccg
     4141 taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct
     4201 ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg cagttggtgg
     4261 ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt ccaggcactc
     4321 gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca tccctttggc
     4381 cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc
     4441 ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg gggccgaaag
     4501 gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg gccatatcag
     4561 cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca ccgggcaacc
     4621 aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt tcgtattcca
     4681 taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac caacgcaagc
     4741 actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc
     4801 gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg ctgacggtgc
     4861 gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg ctggcctcgg
     4921 ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc tgcgcggcga
     4981 taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc atctcgctgc
     5041 ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcgggc agttcgaggc
     5101 tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc tggccagcct
     5161 gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc agcagcaccc
     5221 acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc
     5281 ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc
     5341 gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc catgcctgat
     5401 agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct ttcatgtcat
     5461 ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc tgctcggcgg
     5521 gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc gtgttctgga
     5581 gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg tccctgacgc
     5641 cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc
     5701 tgtcgttctt catcgggcca ccaagcgcag ccagatcgag ccgtcctcgg ttgtcagtgg
     5761 cgtcaggtcg agcaagagca acgatgcgat cagcagcacc accgtaggca tcatggaagc
     5821 cagcatcacg gttagccata gcttccagtg ccacccccgc gacgcgctcc gggcgctctg
     5881 cgcggcgctg ctcacctcgg cggctacctc ccgcaactct ttggccagct ccacccatgc
     5941 cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc tgcgcctcgc tggcctgctg
     6001 ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc gccatgctct gggccagcgg
     6061 ttcgatctgc tccgctaact cgttgatgcc tctggatttc ttcactctgt cgattgcgtt
     6121 catggtctat tgcctcccgg tattcctgta agtcgatgat ctgggcgttg gcggtgtcga
     6181 tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg gccttccatc tccaccacgt
     6241 tcggccccag gtgaacaccg ggcaggcgct cgatgccctg cgcctcaagt gttctgtggt
     6301 caatgcgggc gtcgtggcca gcccgctcta atgcccggtt ggcatggtcg gcccatgcct
     6361 cgcgggtctg ctcaagccat gccttgggct tgagcgcttc ggtcttctgt gccccgccct
     6421 tctccggggt cttgccgttg taccgcttga accactgagc ggcgggccgc tcgatgccgt
     6481 cattgatccg ctcggagatc atcaggtggc agtgcgggtt ctcgccgcca ccggcatgga
     6541 tggccagcgt atacggcagg cgctcggcac cggtcaggtg ctgggcgaac tcggacgcca
     6601 gcgccttctg ctggtcgagg gtcagctcga ccggcagggc aaattcgacc tccttgaaca
     6661 gccgcccatt ggcgcgttca tacaggtcgg cagcatccca gtagtcggcg ggccgctcga
     6721 cgaactccgg catgtgcccg gattcggcgt gcaagacttc atccatgtcg cgggcatact
     6781 tgccttcgcg ctggatgtag tcggccttgg ccctggccga ttggccgccc gacctgctgc
     6841 cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg ctgttgcttt tgcttttcgg
     6901 ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg tggccgttag gccagtttct
     6961 cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag ggtcgggatt gccgccgctg
     7021 tgcctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg
     7081 gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc
     7141 cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg aaacaaggcg
     7201 caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt ggccggagga
     7261 tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg
     7321 tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg
     7381 ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc
     7441 gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc
     7501 cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg
     7561 cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccg
//
