LOCUS       NZ_CP053238             8138 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-106 plasmid pSCU-106-4, complete
            sequence.
ACCESSION   NZ_CP053238
VERSION     NZ_CP053238.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609771
            Assembly: GCF_013085185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:10:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,452
            CDSs (total)                      :: 5,328
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 108 of 269
            Pseudo Genes (incomplete)         :: 154 of 269
            Pseudo Genes (internal stop)      :: 52 of 269
            Pseudo Genes (multiple problems)  :: 47 of 269
            Pseudo Genes (short protein)      :: 3 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053238.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-106"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-106-4"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            complement(<1..298)
                     /locus_tag="HHJ24_RS26760"
                     /old_locus_tag="HHJ24_26750"
     CDS             complement(<1..298)
                     /locus_tag="HHJ24_RS26760"
                     /old_locus_tag="HHJ24_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_170969616.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPG"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(531..2750)
                     /gene="mobQ"
                     /locus_tag="HHJ24_RS26765"
                     /old_locus_tag="HHJ24_26755"
     CDS             complement(531..2750)
                     /gene="mobQ"
                     /locus_tag="HHJ24_RS26765"
                     /old_locus_tag="HHJ24_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029340.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2929..3204
                     /locus_tag="HHJ24_RS26770"
                     /old_locus_tag="HHJ24_26760"
     CDS             2929..3204
                     /locus_tag="HHJ24_RS26770"
                     /old_locus_tag="HHJ24_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(3421..4335)
                     /locus_tag="HHJ24_RS26775"
                     /old_locus_tag="HHJ24_26765"
     CDS             complement(3421..4335)
                     /locus_tag="HHJ24_RS26775"
                     /old_locus_tag="HHJ24_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277132.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(4864..5007)
                     /locus_tag="HHJ24_RS26780"
                     /old_locus_tag="HHJ24_26770"
     CDS             complement(4864..5007)
                     /locus_tag="HHJ24_RS26780"
                     /old_locus_tag="HHJ24_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724382.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGWTVSPSSTAELTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(5069..5335)
                     /locus_tag="HHJ24_RS26785"
                     /old_locus_tag="HHJ24_26775"
     CDS             complement(5069..5335)
                     /locus_tag="HHJ24_RS26785"
                     /old_locus_tag="HHJ24_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000861936.1"
                     /translation="MKVELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFERL
                     TEHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(5355..5612)
                     /locus_tag="HHJ24_RS26790"
                     /old_locus_tag="HHJ24_26780"
     CDS             complement(5355..5612)
                     /locus_tag="HHJ24_RS26790"
                     /old_locus_tag="HHJ24_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523347.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISGNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5622..5762)
                     /locus_tag="HHJ24_RS26795"
                     /old_locus_tag="HHJ24_26785"
     CDS             complement(5622..5762)
                     /locus_tag="HHJ24_RS26795"
                     /old_locus_tag="HHJ24_26785"
                     /inference="COORDINATES: protein motif:HMM:NF020569.2"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_231237649.1"
                     /translation="MYEWDSQHGELEGYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(6226..6369)
                     /locus_tag="HHJ24_RS26800"
                     /old_locus_tag="HHJ24_26790"
     CDS             complement(6226..6369)
                     /locus_tag="HHJ24_RS26800"
                     /old_locus_tag="HHJ24_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(6431..6688)
                     /locus_tag="HHJ24_RS26805"
                     /old_locus_tag="HHJ24_26795"
     CDS             complement(6431..6688)
                     /locus_tag="HHJ24_RS26805"
                     /old_locus_tag="HHJ24_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(6691..>8138)
                     /locus_tag="HHJ24_RS26810"
                     /old_locus_tag="HHJ24_26800"
     CDS             complement(6691..>8138)
                     /locus_tag="HHJ24_RS26810"
                     /old_locus_tag="HHJ24_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_170969617.1"
                     /translation="GGLAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIA
                     KDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPM
                     SVPVVDAKPTERPGVFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQ
                     GGNTRDAVIRFPKDSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERN
                     YERARAELNQANEDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHD
                     PMSGGHRMWQMAGLKAQRAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEK
                     DAKDKLDKESKRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDD
                     FRRKFWEEVSKDPELSKQFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDG
                     GVYDMDNLRITTPKRHIDIHRGQ"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             1691..8138
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2393..2751
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2752..2925
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(3422..4335)
                     /name="rep_cluster_2134"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(535..2750)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.053"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcctggagt ggaaagtgcc ggaaaaccaa atgccactgg cgcagctact gctgacagat
       61 taccgcctgt tcccgagcca ccaccggaat taccattccc cccgccatta ccatgaccgg
      121 aaccacctcc ccagtgaatg ccgctaccgg aaccaccacc ccacgggtta ttttccgaac
      181 tccacccgga accatcagaa gcaccaccac ctacaccaag cccggtcggg ccaccattaa
      241 tgttaccact tgtgctatgc gcgcccgtgt tatggccgcg tccatcgcca ccgctcataa
      301 aaattccctc ttttaagcgt taaaacaatt aattacatga ttaatactgt acataaaacc
      361 actggtttta tgtacagatc aaaaagtacc cgtcatcgtt ttttctgtca agctcaggtg
      421 gaaaattttt tagaaatcag gagggataac acttagaaaa cgcagagcgg gccacaaaaa
      481 attttgtggc ccgtgatgtg ttggcggggt ccggggtgca gccccggaca tcacatactc
      541 atgccccgga agtgcccggg gcggcgttgt tgttgcctgt gctgttcgtc ccgcttccac
      601 tcatgccagg tctgattaaa accgagttga cggaagagat aatccggcat ctcttccggt
      661 ttgtggacag cagccaaaag cctgtcatga tggcctttga gccggaactg catcaggtgc
      721 ggttccggtg ggtacggagt atcctcatca tggtttatag cttctccgta acctgccagg
      781 cataccccgt tctccgtgat ggtgctgact atcagatgac tgtaatccgg cgacggaagc
      841 acaagaacgg ggcaaaaccc tccttcccgg cgaacaaacc cgacaagcat ttcctccggc
      901 cctttgcata acaacagatt cagcgtcctg ctgtttccct ctctcgaaaa tacaacaggc
      961 atgagttgtt ccggtacagc atcagcggtg atatccaccc ggtgcgctga cggcagcgga
     1021 gaggctacgg gcctgcggtc gtcgctgaaa taaaaattat ccggccagtg atgcggcagc
     1081 gccggaaaac cgtaagaggg ccacccctgg cgcatctgtt ccagtacatg ccggaccgtc
     1141 gcaccggaat acattctcac acgctcaggc cgcgccatcg tctcatgaga ttctggcatt
     1201 ccctcacgta tatccgagac catcacagcc ccgcctggcg acaggtaaac attcaccggg
     1261 tcatgaacac tctgggggta atgcgccagc tcatcacccc ataccgtgaa ttctccggct
     1321 ctgttactca gcatcatgta gcgggttatc ctgcccgttc ccggctctct ggcacgcccc
     1381 agctccgtaa taaccccgac taatgccgtt tcacggctca gacggtggtt atcatgacgt
     1441 ttcagccgcg cctcatgctc ctgttccttc cggcgcgctt ccgcttcggc ttcctccctg
     1501 cgccgctccg cttcccgtgc tgacagtttt tctttcagct ctgccattcc cggcgcaatc
     1561 cacgtattcc actgcgtttc aaaggcctga cgacgggcgg gaatgtcccg ttctttcacg
     1621 gccctgtagg tgcggcgaag agattcaatc tgtttcccga gtttccagaa acggagttgt
     1681 cttttgcggt attccggttc agcccatgtc atcaggggaa tctctgacag aagggacgcg
     1741 acagggcgtg cttttttgag tccggtcatc tccttttcga gaatggcctg ctctttcagc
     1801 agcgcattcc cctcacgttc gatgtcgttc accttcccgc tcatcgccag gaactccggg
     1861 gtcatctccc acgcgttgcc gcattcctgc ggcaacgtgg caatgaattc ccgcagggta
     1921 tccgctgagg cattcgcagc gggaatggtc aggttaaggg cttccgctgc acgacgtgcg
     1981 gcatcgctga gttcccgttt ccgcacgttc tcctgtacct ggatgactgc cagccttgtg
     2041 cgggaaacct gtatttccag gttaaggctg ttaatcagac ggttctgctc accgcgttct
     2101 gtttccattc cccgtttttc catcgcacag gcagcctttc cgaggtgaag gccgggcgct
     2161 ttctccagtc cctgacgctc gtatgaacga tggtctatct cttcctggta gccggcgttc
     2221 gcaagcgctc tgttcgcatg gtcagcccat gatgcgcgcc acgtctcggc gagtgcccgg
     2281 tcgttccagt ccctgacctt tcctgcaaat cccgtttccc cgacagctct cgtggtcagc
     2341 atgatgtgcg catggggatt ggtccggtcc atgtgatgga acgccacatc ggcaatcatg
     2401 ccccgactga caaagttttc ccggacaaaa gcgagaacgg tctcccgtgc ggcctcgcgg
     2461 gaaatctcac ggggaatggc gagttcaatc tcccttgcca gctgggagtt ccggcgctgt
     2521 tcggctttct cgaccgcatt ccagagaacg gaacggtcac cacaccagga cggtgcattc
     2581 gcaggggtga gaatgaccgc atcatcaacg ccgcgtttac gggtgtaatc gtgggttaat
     2641 ccggtacggt catcgggtat gacagagccg gaacggtagg cagcagaagc aacggcactg
     2701 tagccgtttt ttcgcgaaat gattttcatg ctgagatgat aaatcgccat ttctgccgta
     2761 atgccttttt gccgttttct tttagcgtcg cagaccgcac acaatcgaaa cgaagtggag
     2821 attgtataag tgcgcacttc gtgatgtgcc gtgcattatg ccagttcagt tttgcaacta
     2881 ccagtgacgc gctaaaatgc gatttttcac cgcgcacgga gtcacgaaat gagcagaaca
     2941 ctcgaacaga agattgccga agctgaggcc agattacagc gcctgaaggc gaagagccga
     3001 agcctggaca cggcgcaaaa agtcattgtg ggggcagcca tgctggcaag ggtacggagg
     3061 ccggaagaag cgcaactgcg tgcgttcctg ctccagtttc tgcgaaagga agtcacaaga
     3121 caggcggatg tgaaccgact acaaccgctg attaacgagc tggagaagct acccagacca
     3181 ccggcgaaac cgcagaacca ctaagccccg gcgcgtaagt gccacatgca ccaccggacg
     3241 cctttgcgct tcctcgctca ttcgctcgct acgctcggac atgagactgc ggcgagcgct
     3301 accacctcaa tcatgcggaa agccacacca ttgcgggatt caacgaggga aatagcaaca
     3361 aaccaagctg ctcaaaaaga cgccaaaaag gcgctgttat ctgatatagg cttatttggt
     3421 ctactctacg agacctcgtt ttttcagttg gtaataccag gcgcgactga tccctaattt
     3481 ctcccaaggt ttcagcgtgc gtgctgatgt agctactgtg ccgcgcttag acttagaacc
     3541 gcccttgcga ccgcgtgtag cctgaatttc tggcgtgtgc gtatcagcca catactgtgc
     3601 gaaagtttcc ggcgtgaagt tcctgtgcgt gtatttcgca atactcttgc caatagcccg
     3661 acattcagga ggtgaaagcg gaacggggag cgatgcgttg tacatttcga cacgctgaat
     3721 caccgcatca agccattgtg agaatgccgg ccagccctga cgaatagccc ggtaagccca
     3781 tttacggccc ttttcgaaca gatagcagtt tcgccccagc ccgtaatttt tatcgacgct
     3841 acggcgcgct gaggcgctca aatcgagata atcagccagt tcgtcgagag tgtagggttc
     3901 ctcgcgccat tccacttcct gccattcagg gtggcacgga tttttgcaga tcaggccgct
     3961 gtaattcaca tccgcgccca gtttttcaca caacgcacgc tcaatagcgg cagcgtatct
     4021 gagcgccgaa gccgatgcat ccggcgcagt tctcacagga agggcgagcg cgtagagcag
     4081 atgcgcgtgc ccgttacggg gattttttac ggtgatgttc ggggccggac aattccggtc
     4141 gctccagtcg attgatgccc ccgtccggtc cacgtcaaag ggaagccagt acagtgcatg
     4201 tggctggttc tgctggatga aacgggcaag aatggcaatg tttttcggca gaatgcgaac
     4261 gccgtacgcg aaatcgttcg tacagtacgg tttgtgcggt aatttttccc tgaagcgttg
     4321 aagtacggcg ctcatgtgat acgatttctc tcggtacaca gtttaactaa ccgcgctttc
     4381 ccatcgggaa acgcggaacc cggcgccctg taatttcgtg atcaatcttg gcggtgagag
     4441 cacgatttac ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt
     4501 cgatccttcc cgaaaaaccg atccatttac cataactccc gttgtacgta ccccttaaaa
     4561 caccctcaat cgctttctga cgcaaaaaac acaaaaagac aaggaaacac cagggtaaac
     4621 ataaaaaatg cgtcagaatc gtttttagcg cgttgcataa ctatgcacaa aagcgataca
     4681 cgatcggttt cgtgcgttct gaacaccgaa cggctcaaaa cgacaagttt tctccccctg
     4741 gatggaaagt tctgcacgag aattttagat cccatctccc acatggaatt tttgtgacat
     4801 ttcaaaaaaa agcgcatcag aatcagcctg acacgctttg tttttgtcaa agaggatttc
     4861 gggttactgc gttgctaatc cggtcagttc agctgttgat gacggggata cagtccagcc
     4921 ctgaacatcc cggatatagt ttgcctgaca tgcagccaga ataatgactg caagaagcaa
     4981 taaaataatc cctgttattt ttttcataac gactccttgt tgtgaaagaa tgccagtcat
     5041 tcatccttaa acaaccggca ttcatatttc agccctgttt aaatcctgac ttaccgttag
     5101 cagctcgcca ttctttaatt tccttgacaa tcccttcagg actatcttcc ctgtcatcgc
     5161 gaggataata aatcagatct gaaccatctg ggtgctcagt taatcgctca aactctctca
     5221 ctaatttatt gtcatcctct tcagtagcac cttcagctct acatattttt tttacaagct
     5281 ccagaaattc agcctcggta taatcactaa tacttttctt tagctccacc ttcatctctc
     5341 ctgagaatat ttgatcacca atcaccatca cgataatcaa atgaaacaaa atactcatta
     5401 ccggaaatat caatttgatg attaaagtat ggttgtagca aaggtaccca ttcagctata
     5461 acatcaaacc aaccattatt gatattatct tttaaaggca ttccaagacg ttcaatgacc
     5521 gagccatcat caccgaaatc ttttgaatac tcaccacctt taaattcctc ggtcgtctta
     5581 tcaaaccaat taatatgtaa tttaagcccc ataacatcct ctcaaagata ctttttgata
     5641 tttcgttttg gatcaggtcc tttcaactga ttaccagtct taggatcgaa tgagccaaga
     5701 tgctgaccat cactggcacg atacccttca agttcaccgt gctgggagtc ccattcataa
     5761 atcttacggc ctttatgggg tccgcttgga attcggaaaa taaaccacgt taagactatt
     5821 ttttagtcaa ataactttca ggaatcgcca gaatggcggg acaacagcgg ttttggtgtc
     5881 cgaaatcgta caattctgta catctacgca aggaaatgta cggaagtaag ccaaatggtt
     5941 atcggacggt cggaacattt cgtacagcct ttacgttcag tgagttagct ggcctagatg
     6001 taaccactcg ttcaactcaa ttctcttagc gtgggaaaat ttccgttttc caagcgcccc
     6061 cccgaatggc gaacaggtca aaatgacaag ttttctccca ccggatggaa ggttctgcac
     6121 agggatttta gatcccatct cccacatgga acttttgtga catttcaaaa aaagcgcatc
     6181 agaatcagcc tgacacgctt tgtttttgtc aaagaggatt tcgggttact gcgttgctaa
     6241 tccggtcact tcagctgttg atgacggaga tacggtcccg ccctgaacat cccggatata
     6301 gtttgcctga catgcagcca gaataatgac tgcaagaagc aataaaataa tccctgttat
     6361 ttttttcata acgactcctt gttgtgaaag aatgccagtc attcatcctt aaacaactgg
     6421 cattcatatt tcagccctgt ttaaatcctg acttaccgtt agcagctcgc cattctttaa
     6481 tctctttaat aacagcttca gggctaccat cattattacc ttctgggtaa taaatcagat
     6541 cagaaccact aggatgctca gtaacactta taaaatgctc gaggttatca tcctgttttt
     6601 tttcatcacc ttcacaattg atgatgtctt caataatttt tttgaattca gtttcagtgt
     6661 aatcactaat gctgtttttc agttccattt ttactgacct ctgtgaatat caatatggcg
     6721 tttcggcgta gtgatacgga ggttgtccat atcataaaca ccaccatcct gactgattgg
     6781 cttgtcgtga tgaagctcaa atgagcgacg cccaccgaca gtatctttat tcctagcccg
     6841 tggagctaat ccttggctca agcgtttttt attacctgga ttaaattgct tacttaactc
     6901 aggatctttc gacacttctt cccagaattt tctccgaaaa tcgtcgaagt ttttaaattc
     6961 tttatcacgc aacttatcag caatgcgatc tggaattggc gctcctgaat ctttacctgc
     7021 atcatccagc catttatcac caactggttt acctttacct gtcgccttcc ctggcttatt
     7081 ccgtttactc tccttatcta atttatcctt agcgtccttt tctttatttt ccttctgttt
     7141 gcggcgctcc tgcgcggcac ttaatgcagc atcagcatct gacttctctt ttgcagcagc
     7201 atcaaatgca gcctgcttat tatttacatc cgtctgcgcc cgctgggctt taagcccggc
     7261 catttgccac attctgtgac cgccagacat tgggtcatgg gcaaatcgat caaattgttt
     7321 tatttcagct attgcatcag caagagtctt attcgctgca tcaagttcgc ttttacgcga
     7381 attataaacc tgaacagctt tagcctgtcg ctcctgattt ctggcaacat cttcatttgc
     7441 ctgattcagc tctgcacgcg cgcgttcata atttcgctca gccgcttcaa ccggatgcgt
     7501 agcatcccat tcctgctgac ggcgattttc ttcatcctga cgttgtttta cctggtcagg
     7561 gctaagaaca tcactcactg aaacatatac ggcattatga ccgctgtcct tcgggaatcg
     7621 aataactgca tccctggtat taccaccctg agtaaatcct gccgggcgaa catccttatc
     7681 agtattattt gtaacacctg ggcttaatgt ctgtactgct ggcgtactgt tattaactga
     7741 aatattcaga acaggtgcac ctggaattga tgccgtaaaa acacccggac gttcggtagg
     7801 ttttgcatca accaccggaa cactcatcgg aacacctgaa acaaccgaaa tattctgtcg
     7861 ttcgtctttt acatcatcaa caacacgaac atttacgttt actgttgcct tatcgagagg
     7921 taatgaactg acaggtgatt cagtaatatc atctgcgggt aatgacgtca caatctttga
     7981 catcatattg gggtcatctt tcgctatttg tgatggcaat acaccatata aagccacccc
     8041 ccaaagacca aatttaaacg gtcctttcag ggcagccata atatcagcaa tagctgccga
     8101 taatgctccc gctgaaatac tgaccgccag accgccag
//
