LOCUS       NZ_CP053237            11467 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-106 plasmid pSCU-106-3, complete
            sequence.
ACCESSION   NZ_CP053237
VERSION     NZ_CP053237.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609771
            Assembly: GCF_013085185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11467)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:10:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,452
            CDSs (total)                      :: 5,328
            Genes (coding)                    :: 5,059
            CDSs (with protein)               :: 5,059
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 108 of 269
            Pseudo Genes (incomplete)         :: 154 of 269
            Pseudo Genes (internal stop)      :: 52 of 269
            Pseudo Genes (multiple problems)  :: 47 of 269
            Pseudo Genes (short protein)      :: 3 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053237.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-106"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-106-3"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            759..956
                     /locus_tag="HHJ24_RS26715"
                     /old_locus_tag="HHJ24_26705"
     CDS             759..956
                     /locus_tag="HHJ24_RS26715"
                     /old_locus_tag="HHJ24_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_028132815.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQTHFEAQYGIEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1963..2094)
                     /locus_tag="HHJ24_RS26720"
                     /old_locus_tag="HHJ24_26710"
     CDS             complement(1963..2094)
                     /locus_tag="HHJ24_RS26720"
                     /old_locus_tag="HHJ24_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001368419.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            2118..2408
                     /gene="cki"
                     /locus_tag="HHJ24_RS26725"
                     /old_locus_tag="HHJ24_26715"
     CDS             2118..2408
                     /gene="cki"
                     /locus_tag="HHJ24_RS26725"
                     /old_locus_tag="HHJ24_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_072149383.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(2405..4051)
                     /gene="cka"
                     /locus_tag="HHJ24_RS26730"
                     /old_locus_tag="HHJ24_26720"
     CDS             complement(2405..4051)
                     /gene="cka"
                     /locus_tag="HHJ24_RS26730"
                     /old_locus_tag="HHJ24_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_165371048.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVVEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGTDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="cka"
     gene            4292..4582
                     /locus_tag="HHJ24_RS26735"
                     /old_locus_tag="HHJ24_26725"
     CDS             4292..4582
                     /locus_tag="HHJ24_RS26735"
                     /old_locus_tag="HHJ24_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330705.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(4574..4852)
                     /locus_tag="HHJ24_RS26740"
                     /old_locus_tag="HHJ24_26730"
     CDS             complement(4574..4852)
                     /locus_tag="HHJ24_RS26740"
                     /old_locus_tag="HHJ24_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330704.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            4974..5396
                     /locus_tag="HHJ24_RS26745"
                     /old_locus_tag="HHJ24_26735"
     CDS             4974..5396
                     /locus_tag="HHJ24_RS26745"
                     /old_locus_tag="HHJ24_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032330703.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7149..10106)
                     /locus_tag="HHJ24_RS26750"
                     /old_locus_tag="HHJ24_26740"
     CDS             complement(7149..10106)
                     /locus_tag="HHJ24_RS26750"
                     /old_locus_tag="HHJ24_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_170969595.1"
                     /translation="MSFLDAKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHWDELIGRQKSLRKLGRLIFSAFSFRAFR
                     PDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVHLRE
                     RLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTLNAK
                     LKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLLAEV
                     HSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVMMDR
                     HLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKYGKR
                     PGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATETTF
                     SMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLRLKA
                     SIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHAILN
                     KGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDYIRS
                     QGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            complement(10087..10710)
                     /locus_tag="HHJ24_RS26755"
                     /old_locus_tag="HHJ24_26745"
     CDS             complement(10087..10710)
                     /locus_tag="HHJ24_RS26755"
                     /old_locus_tag="HHJ24_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000904945.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRR"
                     /name="recombinase family protein"
     Typing          complement(1038..1119)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="93.0"
                     /evalue="3.21e-32"
     Typing          6782..6860
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="87.0"
                     /evalue="7.3e-29"
     Typing          1136..1693
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.405"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttctcctttg gcataatgaa gcgcatcatt ccattttttc catgctccaa tgtaatcacc
       61 ttcacgctcc agtctgacag cttcgtttgc gggtgaaaaa tatttatttt ctaaataaat
      121 tgctttgtcc tgattcatat tcgtaattta tatttttatt tgaattaaaa aagagcgagc
      181 ccatcgggtt aatacatatt ggcatgactg ttgatatatc agcccaacag cacgctcgta
      241 atcgttttaa cgtgattttc gcaacatggc aatccgggtt catctgtccg gtaatcaata
      301 aaaaccgtgc gaaaaacggg acaggatgtg tatttgtcgg accgcgcaca cgcaatcaga
      361 caaatgccgc ctagtcatgg ctgcgccccg acaccccgcc aacgtctctg gccagaactg
      421 gccatcacgc cgggtcactt tgtcagatgt taaaaaatca cgcggtaaac gccgttattc
      481 gcgggtttgc gcaagcacct cccgtgcgtc actttgtcaa aacgtcaaag tgacgcacat
      541 caggaacagc cggaacattc gtgtttatga caacaactgc acacaacgca gacagaaaat
      601 cgcgcacata ttcagcaaaa aataagcgtc acggggtgtt aatgtcgcgc agcacacgcg
      661 cgactgggct tgtgtgataa ttttcagtga cgtcaccggg tcatcacacc aggtgacgca
      721 ctgccggacg taaacaccag taaaggagac gggagaacgt gaacaaacag cagcagaccg
      781 cgctcaacat ggcgcgattt atcaaaagcc agagcctgac gttgctcgaa aaactggatg
      841 cactggatgc agacgaacag gccgccatgt gcgaacgact gcacgaactg gcggaagaac
      901 tccagaacag catacagact cactttgaag ctcagtacgg catagaacaa ccataaaggc
      961 gctggcgaaa gtggtcgcgg cttgtccgtg agtgttttcg ccagcagaca aagcccgtca
     1021 gggcacgtca gggggcttta gcgggcgtcg gggcgcagcc atgacccagt cacgtagcgg
     1081 tagcggagtg tatactggct taatcatgtg gcatcagtgc ggattgtatg aaaagtgcac
     1141 catgccgggt gtgaaatgcc gcatagatgc gtaaggagaa aatgcagctt ccggtacact
     1201 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg agcggagtca
     1261 cctcactcaa agacggtgat accgttatcc acagaatcag gggataacgc cgaaagaaac
     1321 atgtgcgcaa aaaacgagaa cccggaaaag gccgcgccac tggcgttttt ccataggctc
     1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1441 ggactataaa gataccaggc gtttcccccc ggaagctccc tcgtgcgctc tcctgttccg
     1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaaacgt ggcgctttct
     1561 catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1681 tccaacccgg taagacacga cttaacgcca ctggcagcag ccattggtaa ctggatagtg
     1741 gatttagata cgcagaactc ttgaagttga agccttatag cggctacact ggaaggacag
     1801 catttggtat ctgtgctcca ctaaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1861 gatccggcaa acaaaccacc gttggcagcg gtggtttttt tgtttgcaag cagcagatta
     1921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
     1981 cgtcagttca gaagacgaag atggtgcaac ggttcctcct tgtacatcac gaatatagtt
     2041 tgcttgacat cctgacaaga ataatattgt aattagaaaa attactattt tcatacacct
     2101 ccaacaaaag aaaaattatg tatctaaaat actacctaca taatttacct gaatcactta
     2161 taccatggat tcttatttta atatttaacg acaatgataa cactcctttg ttatttgtct
     2221 ttatatcatc aatacatgta ttgctatatc catactctaa attaaccata tctagatata
     2281 tcaaagaaaa tacaaagtta aaaaaagagc cctggtactt atgcaagtta tctgcattgt
     2341 tttatttatt aatggcaatc ccagtaggat tgccaagttt catatattac actctaaaga
     2401 gaaattaaat ccctaacaac tcattaagtt tgttcaactc atcatcccct atgtaagaag
     2461 aaacaatacc tgtgacaatt gcaatccccc agaaaccaag aggcacacca acaataaaac
     2521 taaacattaa tgccacaatt tttgccacac ctacatctac cgcacttttc tccagcgtga
     2581 caaacaaagg tcgccagtta ccagtttcta ttgcattttt aaaatcagta ccaacatcat
     2641 atagaaaaga tactcgacta gtgattttaa gcgactttga tatcttcgtt aaattcttag
     2701 ataactcatc ataattaaca gactctaacg cattaaaaat agcatcccga tcaaccttac
     2761 tgaatttctt atccagtaca tttttatact tttcaaatgc tgccagagct tcatcaacac
     2821 cttgaatttt ctttccttta gacttttccg ctaaatcctg agcaattttt gcgtattttt
     2881 ctccatattg ttcggttata tattgataaa atccaaccat agtatcaaca gcatctttta
     2941 tctggctctt ttcaagagca tcctgagctt cttttaattt ctgctctgcc tctttaacct
     3001 cagcggcctt accatccctg aaactaacag cattcttaag ttcattttca actgcggagt
     3061 actcagcctg cttagcttca agctgacgct gtagtttttt ctgaacgtca cgccatcccg
     3121 gaaatccggt gaccttcacg tcaacactct tctgagcgtt ttcaagctct cctgcaactc
     3181 tggaaacagt agcttgctta ctctgtacat cactttcagc cttcgccagt tcagctttcg
     3241 cctcagctaa acgcttctct gcttccacca ccgcctgttt ctcttcatta agagtttcat
     3301 gttcagcgac ctctgcattt tcacgagcct tttcttcagc ttccttctgt ttattcacat
     3361 caccaatttt actttgcaat gtatttttat aagaattaag tttgctaata tctgcatcaa
     3421 gctgatttga ttttttttgc aactcatcaa catccctctc aagatcagtg ataccatgat
     3481 aagaatgatg cttgaatact tttttcatct cctcgatttt cttctgtttt tcactaatct
     3541 gtgtagcaat tttattcttc tgcttttgtt tttcattaat tacattaccc accaccttcg
     3601 aactcttatc catatcactg acctgagcat tcgttggtgc agtattaact gaagcagtgt
     3661 tattgttgtt atttcctgag ctttctgcaa aaagtgatgg catatcacta attaaagaat
     3721 taagaactct ggagacccct ccaaatggat tatcaaccag agttgaattc tcttctgtca
     3781 taacaatacc attcatcacc ggaaggccat cattattaat gacaacatca ccccacggag
     3841 tcagatatga ctcaccggtt ttcatcacag ttgatgtaga accagatgaa tttgaatttc
     3901 cctgaccacc attattacca tgcccagagc caccacccca gtgaacccca ctattactat
     3961 tattatttcc accttgctga ttcagattcg cccctgttcc ccccatagac tcaccagcag
     4021 ttgggccata tccacttagt tctttagcca taaattcctc tttgataatt aaaacaataa
     4081 attaaaaaca atatactgta tatataacca ctggttttat gtacagtgaa aacctactac
     4141 tcagcattgt ccgtgtcaag agcatggatt ttcatttttg caataaggat cacactatgg
     4201 ggaggcaggc attgagaacg tcgaaacaga acaccggagc aaatcaggat gagatataaa
     4261 actgttggat catgaaaaaa cggagaacga tgtgagcaaa tcaccccgcc ataaactgaa
     4321 caaaacagac aaacgacttc tcgacaccct tgttgctgcc ggatatgagt atgacaaagc
     4381 ccgtgacctc atccagaaac aggtttacac gctgacactg gctgatcagc gtcatgtggt
     4441 cagtgaaatc agtaatggtg tgaatcccac ccaggcttac tcagcggtat accaggcaag
     4501 acgcattcgc ctcgcccgta aatatctgaa cggtaaaaag gttatggaag aaaccgggga
     4561 aaatacgccc ccatcagcgt aaggatttct tttgccgctc cagagactcc agttttttac
     4621 gcaaatcctc tcttttttgg gcatctctgg tgccagccag ctctgctctc aactcattga
     4681 tctgaagttg tatcttcagt ctgttactga acattttctg tctggcatta acatccgcaa
     4741 caatgccgtt ttttgtcttc tcggcctttt gttgaaaaac attgctgtcc gcatgactgg
     4801 caaccgaagc agaaagaaca ctaaaaagca ggactggcac acattttttc acgggattat
     4861 tcctgactca ttgaccatca aatcacattg ggagtaaacc gacgtatgat aagagatact
     4921 cttcggagat ataactccct gagtatcaag attaaaaacg caaggagatg tttatgagat
     4981 ctgccgctgc caggctgctt ctgatacctc tgataacagc aacaatagct cttacaggat
     5041 gcacaccaaa gaccagcctg gaacgacata cccggcatta tgtttatgct tcagatgatg
     5101 gatttgatcc taacttctac acccagaaat cagacaccat acgtatgatg ctccctttct
     5161 ttcagcagtt ccgggatatg gggatgaaag acaaagcagc cggagtatca gcagaaacgg
     5221 cacagcaacg tgtaaaagaa ttccactcag aaaaattttt tcactcactc cggagcacaa
     5281 caacctttgc tggcagaaaa tacacaaaca gcgatatgcc ttcgccgaaa aaaatgaaac
     5341 taatggcaga caccatttct gcggtttatc tcgatggata cgagggcaga cagtaaggga
     5401 tttaccataa tcccttattg tacgcaccgc tgaaacgcgc tcagcgcgat tctggcgcaa
     5461 aaaccgcaaa aagagatgtt ttcccccggg taaaccggaa aaatacgtca ggattgtttt
     5521 catggcactg catgactatg catgaaactg aatggcgatc ggtttgggcg cgcctgatgc
     5581 ccataaggcg catttcggac gttttcagcc ctgatgagaa gaaatcagac agtagttaca
     5641 gacgagtcgt gagcgattca ccccgggagt catcagcgag tcatccagta tttttcctcg
     5701 cgactctctg gcgactcgct ttttctgaac accagagaga cagtgtgttg agtcatcgat
     5761 aaatcaccga cgactcgttg ccgaatcatc cagtagtcgc cgacaactcg cttttgtata
     5821 aatctgaata agaaaatata tttttcaaac cataacaaca tgatttaaaa agcaaatcag
     5881 aaaaaagttg gttttgcgtg gggggtgggc atccggggca tgagaacaga ctcgcgtttt
     5941 tctggagaaa ctgcggggat ttttgattaa acaatactca tcgcagagcg gaattttatg
     6001 caacgccggc tgtgcggcac agggattttt aatccccagc atgtcagagg gtgcggatga
     6061 cggttcccgt ctggcgtttt catgggcgcc ggttgtcaaa aaataccagc gtgatcggtc
     6121 gaaggataag cgcagattaa tcagtgatga ttgagcgcga atctgacaac gagtatagcg
     6181 acacagcaac aaaaaagacg ccagaaacgc gctcagaggc tttgtaacgc gattttttac
     6241 atgaggcaat ggtttcgctt tgaggcgttc agccgtgttc agggcgcgat tctggtacat
     6301 acctgactgt aatcatccct acgaactact ctttgcacca gaaacgcgca gggtgtttta
     6361 gcgtgtctcc ggcgtgatgt cgagttgtgc ggtaatgagt gagcgaaaaa aatcccctgg
     6421 attacagggg gaacccatta tcagactttg aagtttattt gttatttgat ggagtgagct
     6481 cgaatcccag agatgacgtt caggtggcaa ggaaacgcgc tcagacgctt tctaacgcac
     6541 ttttttgcat ctggcaatgt ttcattatgt tttggtgggt tcaggctgtt agagacgttt
     6601 tgagcgcgtt tctggcgctt gctgctactg ccaaaccatc cccatggcca gttgatgcca
     6661 gaaacgcgca ggagttttgt agcgcgtctc cggtccctga tcaatgaatt cccggtcatt
     6721 aacatgcgtt tacccggaaa cgcgccctga tgttctatcg tatcgcggga acggtgtcga
     6781 ggctgtcggg gcaaagccct gaccaggtgg taatcgcata gccgtgcgtg cgcggttata
     6841 cgattacaca tcctgtcccg gaatcaggat tgtttttcgc gcacaactgc ggcaaattca
     6901 tcccgttgcg gttgcaacga tgggtatatc aaaactacga aaattattca ggcatttgct
     6961 accccccttg ccctggcctc caccagtcac gggggatttt tttgttgtct ctcatgacga
     7021 actacggcaa atttctgacg ttgcaaactg caacacataa aacacacaca aaactcacaa
     7081 ttgccatgtc ctcattgaca cccccttgcc atataggggt tccgtgggca tttctgcgat
     7141 aacacacgct aaggaaaagt gaagttcttt ggattgaagc gattagtacg aagtggcctg
     7201 tatcgttcaa ggggatggtc aatttcgttc cacaggtaat ctccagtgag cgagatatga
     7261 ccccatggta gtggtgatac actggacaag agctcttccg gtataatgat cccctggctg
     7321 cgaatgtaat caacagcccg actgagatag accgtgttcc acagaattat tgcattaaca
     7381 acaaggttga ggcccgatgc cctgtgagcc attgtctcgg cagccctgtt ccggaattcg
     7441 cccagttggt gaagaaaaat agcgcgggca agtgcatggc ggctttcgcc tttgttgagg
     7501 attgcgtgtg atctgcgacg taaccgagtg tcaagcaacc agtcacagat aaaaattgag
     7561 cgttcaatac gtcccatttc acgcagggcc agattgagcc tgttttgtct cggagatgct
     7621 gccagctttt tcagtattac cgatggtggt accagaccag cgccgatcga ggccttaagc
     7681 cgcagaactt catcccagtg ctgttctatt acttccattt tggcctttcc ggcgatcagg
     7741 gcatcaagtg gtttatattc ctctgagggg gaaataacgt acaacctgcg actgccgagg
     7801 ttgcgaatac ggggtatgag tctgtaacca aaagagtgaa acattgaaaa tgttgtttca
     7861 gtcgcaccgg ctgtatcggt ggcatgctca tgaattacca cagaactttc gttatgaagt
     7921 aagccgtcaa gtacgtaggg agcctcgctt tcagaggcct gaatcattct ggaaaaaaag
     7981 gatgcaaaac ggttcgataa aaaaccataa atcgacgcgc caggtcgttt gccgtatttg
     8041 gcgttatatt cgaggctggc ttctcctcgt ccacccgcag gaaaaaactg cccgtccgag
     8101 gatgacacat gcccatcccc ccatatagca gcaaaaggat gttcctgttg cgcatcgacc
     8161 agaactgatg tggcagacgc ataggtttca gtgcgtaaat gacgatccat cattaccatc
     8221 atctggtgaa tggtgacacc ccgggaactc tgcgccatcc gttctgctcc cgcattcgtc
     8281 gcatcggcaa ggatcgcagc cataagtgca gcttcatctt ccgcctgttc cccggtgcga
     8341 taatgggtga aacaatcaag aaaactggtc cagctatgaa cttctgccag caaactggta
     8401 attctgatgc gtggtaccag catgtagagt ttacggctga gagccttaat tttgtcgctc
     8461 tcttcttcgc gaatcggaga cacagacaac cctctgctgg aaagttctgc atcgggtatg
     8521 gcattctccg aagcagcttt tgccagcgct ttcagttttg cgttgagtgt ggtggttcgc
     8581 tcagcccgcc attcagcaaa gttgtccgga atggcaagtc ccagccggga ttctgcccgc
     8641 atctgcccga aaacatggtg aggaagaagg tagtcctcaa aactacgcca ggcccgacta
     8701 ccatcaaccc atatgtcacc tgttttcagg cgttcccgca aatgaacgaa tacggcgact
     8761 tcccacgcct gcagatcggt attttctcca tggatgacac gacgtcgcca tttatgggac
     8821 ataaaatcga gcggaatttg tttgggtaat tttcggccat gatagaacgc ctgcaaatac
     8881 ccaatcgcca acaaaacgct atcatccggg cgaaaagctc tgaaagaaaa agcgctgaaa
     8941 atgagtcgtc caagcttacg taatgatttt tgtcgtccga taagttcatc ccagtgatgg
     9001 gaagatgcca ccgaagctgc agcctccata ctggcaatca aaccttccca gccgagtgat
     9061 gctgttatta cagattcaat atcagtatta ccgtcacgtg ctgccaggag agcccgtccc
     9121 aacttaatat gatcctgagc tacgacttca agtacttcag cggctttcag acgacgctct
     9181 ttctggctta tctctgctct gcgcctcgtg ctgccaatca atttgcagaa catatcaatt
     9241 gccagatctg taattgctgc ctgcttttca ataacaaagg ctaccagcgt ggcatgacag
     9301 cgttcggatg ccattcgttg tatttcacgc gcatgtaaaa cgcgagaatc ccgggcaata
     9361 ataccgtaac ggtttacatg aatccgttta cgttgctcat cggagattgc cgctgttcgt
     9421 aatacctcca gcctggcaat taacccctta agattcttga gtttcacgcc ttccggcgct
     9481 tcagctatcc agcctaaaat gctcttttct ccgactcgtt cggtaagcaa ctgctcgaga
     9541 gcgctaatgg atgaaggttt cagatccctg atgagttccc gataagaaag gcgacgggca
     9601 gccgcccggc ccgccattgc cagacggatg agttgtgccg gagcaggggc aagtaacttc
     9661 tggtccttca gcttattgat aactgctctg gtgatcggca gtcctttttc ggtggttgct
     9721 gcttcgcgtg tagccgcgat aatgagcatt ctgtaatcgc tactccggac ggaacgaagc
     9781 tccaaagccg aaactatttc gcgggcatgt tcccggcggg tttcctcacg ttgtgcgtaa
     9841 agtgtaaaaa cagcagggtc aatatccagt tgttccgcaa caacggtgat aacagcctcc
     9901 gggggaatct ctcctgcacg cagtgtacga ccgagatcac ggacaagtgc aagctgaatg
     9961 gcaaaaccca gacggttatg agatcgacga tgagttctgg cgaatgcaat atcctcgcag
    10021 gagagcgaaa accggggaag tgcatcctca taaagttcag gtaatccaaa aagcgtatcg
    10081 tacgatttag cgtcgagaaa tgacattgct ctgatccgtt gtcagattac gccccccagg
    10141 tccggcccag ccaattcgtc gtagagtatc ggtgagcgtt gagcgtggca cactgaatgc
    10201 gcgacatact gacgctttac tggcacctcc ttccagagct gcgaggattt gatcaacctt
    10261 ttctgcatct atcgccggtg gccttccgcc ttttctcccc cgccggagag cagcagccag
    10321 accagcatta acgcgttctg tgataagtgt tctttcgtac tccgcgagcg tgccaaaaag
    10381 gttaaagagg aacgagccgt gtggcgttgt tgtatcaata gattcagtca atgatcggaa
    10441 agcaatacca cgaattttca ggtcttcaat aattcggagc aaatgtgaga gtgaacgacc
    10501 aagtcgatcc agtttccaga ctatcagtac atcaccttcg cgaagttcag cgagacagga
    10561 cttaagtccc gggcgattat cacgagcacc tgaagcatga tcctgatgta aatgccgggg
    10621 atccactcca gcagcaatca acgcatcacg ctgcaaagaa actgactgac gttcatcatt
    10681 gcttgatacc cgcatataac caatcaacat gtacgataaa ccttaaaatt gaagtttccg
    10741 tacagtttct cattccgaca tggtttgtca cgcaaaaaat cacagattta tggattcaat
    10801 tttcttcaac tgcaggacct gacggaaaac aagtgatttc cgtcagggag gaatcgctta
    10861 gcgggtgtta tcgcagaaat tcccacggaa ccccatatag ggggattttt tcgcctgtcc
    10921 tgattgtctt ttgccgatgg ttacggcaga ttggaaaaga gcgcatttgt ggtgctctct
    10981 ccaaaataat atttctcatt actcaaactc tgatggcttc cccctgtaat ccagggggat
    11041 tttttaattt atcgctatgc ttcgtaattc attcagaata cagacaatat ggttaatcag
    11101 tttttcatca tttttgcggt gtttgccctg tcctgcatca gaacgctaaa aaaacaggaa
    11161 tctctttatg acaaatcctc catttcagga ttttttatgt agcgtctact acatgcgatc
    11221 cgttggtgag ctgtttatta tgcattagtt atataataac tacatattag ttatactttt
    11281 gttgtgtatc agttgcaggg gcgttttgag ggtaaattct ggaggcacgt gaccgactgt
    11341 atacaaaatg ctggcgcatt ttgatacgtc gacgcgccaa agtggacgcc cctttgaaac
    11401 cccgccagaa ttaaaagcgg cctgtctttt tacagtattc agctctggct atacaccaac
    11461 ctatatt
//
