LOCUS       NZ_CP053039             8312 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain P35 plasmid pP35D, complete sequence.
ACCESSION   NZ_CP053039
VERSION     NZ_CP053039.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14770898
            Assembly: GCF_014524525.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Martins,W.M.B.S., Nicolas,M.F., Yu,Y., Li,M., Dantas,P., Sands,K.,
            Portal,E., Almeida,L.G.P., Vasconcelos,A.T.R.L., Medeiros,E.A.,
            Toleman,M.A., Walsh,T.R., Gales,A.C. and Andrey,D.O.
  TITLE     When KPC- 2, carried by an IncQ1 ubiquitary, wide-host range and
            high copy plasmid, meets the international ST15 Klebsiella
            pneumoniae clone: clinical and molecular description
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Martins,W.M.B.S., Nicolas,M.F., Yu,Y., Li,M., Dantas,P., Sands,K.,
            Portal,E., Almeida,L.G.P., Vasconcelos,A.T.R.L., Medeiros,E.A.,
            Toleman,M.A., Walsh,T.R., Gales,A.C. and Andrey,D.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2020) Medicine, Universidade Federal de Sao Paulo,
            Pedro de Toledo Street, 781, Sao Paulo, SP 04039-032, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-MAY-2019
            Assembly Method                   :: Unicycler v. v0.4.0
            Assembly Name                     :: P35
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:36:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,561
            CDSs (total)                      :: 5,437
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 96 of 199
            Pseudo Genes (incomplete)         :: 109 of 199
            Pseudo Genes (internal stop)      :: 27 of 199
            Pseudo Genes (multiple problems)  :: 30 of 199
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053039.1.
            Bacteria and source DNA available from Laboratorio Alerta,
            Universidade Federal de Sao Paulo; Pedro de Toledo Street, 781, zip
            code: 04039-032, Brazil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="P35"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pP35D"
                     /geo_loc_name="Brazil: Sao Paulo"
                     /lat_lon="23.597500 S 46.643600 W"
                     /collection_date="2015-05"
                     /collected_by="Laboratorio Alerta"
     gene            1..840
                     /locus_tag="HKJ75_RS27510"
                     /old_locus_tag="HKJ75_28100"
     CDS             1..840
                     /locus_tag="HKJ75_RS27510"
                     /old_locus_tag="HKJ75_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="HKJ75_RS27515"
                     /old_locus_tag="HKJ75_28105"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="HKJ75_RS27515"
                     /old_locus_tag="HKJ75_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            <2290..3153
                     /locus_tag="HKJ75_RS27520"
                     /old_locus_tag="HKJ75_28110"
                     /pseudo=""
     CDS             <2290..3153
                     /locus_tag="HKJ75_RS27520"
                     /old_locus_tag="HKJ75_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(3403..4284)
                     /locus_tag="HKJ75_RS27525"
                     /old_locus_tag="HKJ75_28115"
     CDS             complement(3403..4284)
                     /locus_tag="HKJ75_RS27525"
                     /old_locus_tag="HKJ75_28115"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="WP_004199234.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-2"
     gene            complement(4593..5093)
                     /locus_tag="HKJ75_RS27530"
                     /old_locus_tag="HKJ75_28125"
     CDS             complement(4593..5093)
                     /locus_tag="HKJ75_RS27530"
                     /old_locus_tag="HKJ75_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_029749167.1"
                     /translation="MRALKDAGVKANRIFTDKASGSSTDREGLDLLRMKVEEGDVILVK
                     KLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRIL
                     ERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDE
                     RAS"
                     /name="recombinase family protein"
     gene            complement(5190..5474)
                     /locus_tag="HKJ75_RS27535"
                     /old_locus_tag="HKJ75_28130"
     CDS             complement(5190..5474)
                     /locus_tag="HKJ75_RS27535"
                     /old_locus_tag="HKJ75_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            5673..>6314
                     /locus_tag="HKJ75_RS27950"
                     /pseudo=""
     CDS             5673..>6314
                     /locus_tag="HKJ75_RS27950"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6421..6834
                     /locus_tag="HKJ75_RS27955"
     CDS             6421..6834
                     /locus_tag="HKJ75_RS27955"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6933..7802
                     /locus_tag="HKJ75_RS27960"
     CDS             6933..7802
                     /locus_tag="HKJ75_RS27960"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7863..8075
                     /locus_tag="HKJ75_RS27545"
                     /old_locus_tag="HKJ75_28140"
     CDS             7863..8075
                     /locus_tag="HKJ75_RS27545"
                     /old_locus_tag="HKJ75_28140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            8077..8283
                     /gene="urfF"
                     /locus_tag="HKJ75_RS27550"
                     /old_locus_tag="HKJ75_28145"
     CDS             8077..8283
                     /gene="urfF"
                     /locus_tag="HKJ75_RS27550"
                     /old_locus_tag="HKJ75_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             complement(3407..4284)
                     /name="blaKPC-2"
                     /gene="blaKPC-2"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5429..5584
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          5674..7799
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 cccctgttta acaatcacgc cccccccccg gtaactgtca cgaacccctg caataactgt
     1741 cacgcccccc tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg
     1801 caaacccagc aggggcgggg gctggcgggg tgttggaaaa atccatccat gattatctaa
     1861 gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat
     1921 atgcccggcc agaggccgga tagctggtct attcgctgcg ctaggctaca caccgcccca
     1981 ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa accccacacc aaaccccgca
     2041 gaaatagggg tgtgacgccc aatggaacga aaacgtacgc taacaaaaaa caagcatgtc
     2101 atttaagtca tgacacagcc agtagtgaca cataggttat gtcatatcct gattttgggg
     2161 tgctttttac cagtgaccgt agggtctacc taagcgtcat accgttctaa ctgtttgatt
     2221 taatgcttaa cgtacgaaac cgtaccgttg ggcttcacac cccaatacga agccgccaaa
     2281 catggcgacc acaaggcgat gagctataag cgcatgaagc cggccgagga cgaattgcat
     2341 tgcgagatca aggcgctgct tgatcgcgcc aaggctaccg acgaccagga gcgtaacgag
     2401 ccggagctgg acattcctgc cgagatttct cgccgcgaga agcgcctgga ggcgatccag
     2461 gcggcaaagg cgcgcctgga agcgcgccag cgtgaagcgg accaggcccg ggggcgcagc
     2521 gaagacgatg gccgccggcc tcgccatccg gatggctcgg acaagggcgg tggctcgtac
     2581 aaacgcgagt ttggtgtgcc ggatgaccgt gatcaggaaa gcttcaccga tccggacagc
     2641 cggatcatga aacacgccgg tggtggctcc gagcagagct acaacgggta cacagcggtc
     2701 gatgccgagc accagatcat cgtggcggcg gagttgacca actgcgccgc ggacagtcag
     2761 gcgctgctgg gcatgctggc agcagttcag gccaacaccg gagaaatgcc ggcccagacg
     2821 ctggcggatg cgggattccg tagtgaggct gttctggcaa aggtcgccga tcaccacggc
     2881 gatgtcatcg ttgccctcgg ccgcgaggga cgtgaagatg ccaaggtcaa tgccaagacc
     2941 catccgcata cggcggcgat tgcggcgaaa ttgaaaacgg agcaaggcga tgcagcttac
     3001 cgccggcgca agtcgatcgt ggaggctccg aatggttgga tcaaggcggt gatgggattg
     3061 cgtcagttca gcatgagggg cctggacaag gtgcaagccg agtggaagct cgtctgcatg
     3121 gcgctaaatc tgaggcgaat ggcgtatctg tgagggcgaa ggttaaatgg ggcggctcaa
     3181 atgcacccca gtcgtcataa cacgccgcgc gccgcaagat tggtatcctt ggcgccgacg
     3241 ccttgccaat tgcagagagc gccgccgcca tcgtcagtgc tctacagaaa accggtcaca
     3301 cggctctgcc gcgcagactc ctagcctaaa tgtgacagtg gttggtaatc catgccgccc
     3361 gcaagggcgg cggtggtggg ccaatagatg attttcagag ccttactgcc cgttgacgcc
     3421 caatccctcg agcgcgagtc tagccgcagc ggcgatgacg gcctcgctgt gcttgtcatc
     3481 cttgttaggc gcccgggtgt agacggccaa cacaataggt gcgcgcccag tgggccagac
     3541 gacggcatag tcatttgccg tgccatacac tccgcaggtt ccggttttgt ctccgactgc
     3601 ccagtctgcc ggcaccgccg cgcggatgcg gtggttgccg gtcgtgtttc cctttagcca
     3661 atcaacaaac tgctgccgct gcggcgcagc cagtgcagag cccagtgtca gtttttgtaa
     3721 gctttccgtc acggcgcgcg gcgatgaggt atcgcgcgca tcgcctggga tggcggagtt
     3781 cagctccagc tcccagcggt ccagacggaa cgtggtatcg ccgatagagc gcatgaaggc
     3841 cgtcagcccg gccgggccgc ccaactcctt cagcaacaaa ttggcggcgg cgttatcact
     3901 gtattgcacg gcggccgcgg acagctccgc caccgtcatg cctgttgtca gatatttttc
     3961 cgagatgggt gaccacggaa ccagcgcatt tttgccgtaa cggatgggtg tgtccagcaa
     4021 gccggcctgc tgctggctgc gagccagcac agcggcagca agaaagccct tgaatgagct
     4081 gcacagtggg aagcgctcct cagcgcggta acttacagtt gcgcctgagc cggtatccat
     4141 cgcgtacaca ccgatggagc cgccaaagtc ctgttcgagt ttagcgaatg gttccgcgac
     4201 gaggttggtc agcgcggtgg cagaaaagcc agccagcggc catgagagac aagacagcag
     4261 aactagacgg cgatacagtg acatcaacga tattccttgt ttgaaggtgg agctaggtgt
     4321 agcggccgga tgtaatcagg ccgctacagc tgggattagg ggtctgacgc tcagtggaac
     4381 gaaaactcaa cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4441 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4501 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4561 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     4621 ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc
     4681 ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt
     4741 cctttcagct ttgcttcctg tcggccctca ttcgtgcgct ctaggatcct ccggcgttca
     4801 gcttgtgcca cagccgacag gatggtgacc accatttgcc ccatatcacc gtcggtactg
     4861 atcccgtcgt caataaaccg aaccgcgaca ccctgagcat caaactcttt tatcagttgg
     4921 atcatgtcgg cggtgtcgcg gccaagacgg tcgagcttct tcaccagaat gacatcacct
     4981 tcctccacct tcatcctcag caaatccagc ccttcccgat ctgttgaact gccggatgcc
     5041 ttgtcggtaa agatgcggtt agcttttacc cctgcatctt tgagcgctct gatacgctgg
     5101 agcgctttta gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca
     5161 gccccgcagg gcctgtctcg gtcgatcatt cagcccggct catccttctg gcgtggcggc
     5221 agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc
     5281 cgatcctccg gccactcgct gctgttcacc ttggccaaaa tcatggcccc caccagcacc
     5341 ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac ccgctgaatt
     5401 tcggcattga ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc cgccttgttg
     5461 ctccccttaa ccatcttgac accccattgt taatgtgctg tctcgtaggc tatcatggag
     5521 gcacagcggc ggcaatcccg accctacttt gtaggggagg gcgcacttac cggtttctct
     5581 tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg
     5641 gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa
     5701 ccggcagcag gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag
     5761 gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt
     5821 tcgtcgagcg gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc
     5881 ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg
     5941 cgctggcgtc cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg
     6001 ccatccatgc cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca
     6061 atgacggcat cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg
     6121 agaagggcgg ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc
     6181 gcgaggcatg ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca
     6241 ttgaccacag aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc
     6301 cgaacgtggt ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga
     6361 acatcgacac cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc
     6421 atgaacgcaa tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc
     6481 gaaccgctgg cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag
     6541 acccagcagg ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg
     6601 gcggcatggg tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc
     6661 gcgcagagcg cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg
     6721 ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg
     6781 acgccactga caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg
     6841 acaggacttt gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata
     6901 tcggcgtcag ggacgccacc accggccaga tgatgaaccg ggaatggtca gccgccgaag
     6961 tgctccagaa cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca
     7021 ggcccgccga gcaggagcgg catggtctgg tgctggtgga cgacctcagc gagtttgacc
     7081 tggatgacat gaaagccgag ggccgggagc ctgccctggt agtggaaacc agcccgaaga
     7141 actatcaggc atgggtcaag gtggccgacg ccgcaggcgg tgaacttcgg gggcagattg
     7201 cccggacgct ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg
     7261 gccgcttggc gggcttcacc aaccgcaagg acaagcacac cacccgcgcc ggttatcagc
     7321 cgtgggtgct gctgcgtgaa tccaagggca agaccgccac cgctggcccg gcgctggtgc
     7381 agcaggctgg ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg
     7441 ccagcctcga actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt
     7501 accgcagcga gatggccggg ctggtcaagc gcttcggtga tgacctcagc aagtgcgact
     7561 ttatcgccgc gcagaagctg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca
     7621 tggccgaggc cagcccagcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg
     7681 agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg
     7741 cacgggcacc ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt
     7801 agtgcttgcg ttggtactca cgcctgttat actatgagta ctcacgcaca gaagggggtt
     7861 ttatggaata cgaaaaaagc gcttcagggt cggtctacct gatcaaaagt gacaagggct
     7921 attggttgcc cggtggcttt ggttatacgt caaacaaggc cgaggctggc cgcttttcag
     7981 tcgctgatat ggccagcctt aaccttgacg gctgcacctt gtccttgttc cgcgaagaca
     8041 agcctttcgg ccccggcaag tttctcggtg actgatatga aagaccaaaa ggacaagcag
     8101 accggcgacc tgctggccag ccctgacgct gtacgccaag cgcgatatgc cgagcgcatg
     8161 aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg acgacgaata cgaggcgctg
     8221 cgcgagtgcc tggaagaact cagagcggcg cagggcgggg gtagtgaccc cgccagcgcc
     8281 taaccaccaa ctgcctgcaa aggaggcaat ca
//
