LOCUS       NZ_CP053020             5596 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN361 plasmid pKPN361-4, complete
            sequence.
ACCESSION   NZ_CP053020
VERSION     NZ_CP053020.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14752225
            Assembly: GCF_016804025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Du,N. and Du,Y.
  TITLE     Genome sequencing and assembly of Klebsiella pneumoniae strain
            KPN361
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Du,N. and Du,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2020) Clinical Laboratory, The First Affiliated
            Hospital of Kunming Medical University, Xichang Road, Wuhua
            District, Kunming, Yunnan 650032, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 872.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,758
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 55 of 151
            Pseudo Genes (incomplete)         :: 102 of 151
            Pseudo Genes (internal stop)      :: 21 of 151
            Pseudo Genes (multiple problems)  :: 23 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN361"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN361-4"
                     /geo_loc_name="China:Yunnan"
                     /collection_date="2016-10-03"
                     /collected_by="The First Affiliated Hospital of Kunming
                     Medical University"
     gene            548..772
                     /locus_tag="HJ591_RS28525"
                     /old_locus_tag="HJ591_29275"
     CDS             548..772
                     /locus_tag="HJ591_RS28525"
                     /old_locus_tag="HJ591_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            756..1022
                     /locus_tag="HJ591_RS28530"
                     /old_locus_tag="HJ591_29280"
     CDS             756..1022
                     /locus_tag="HJ591_RS28530"
                     /old_locus_tag="HJ591_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1092..1442)
                     /locus_tag="HJ591_RS28535"
                     /old_locus_tag="HJ591_29285"
     CDS             complement(1092..1442)
                     /locus_tag="HJ591_RS28535"
                     /old_locus_tag="HJ591_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1508..2053)
                     /locus_tag="HJ591_RS28540"
                     /old_locus_tag="HJ591_29290"
     CDS             complement(1508..2053)
                     /locus_tag="HJ591_RS28540"
                     /old_locus_tag="HJ591_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2079..2405)
                     /locus_tag="HJ591_RS28545"
                     /old_locus_tag="HJ591_29295"
     CDS             complement(2079..2405)
                     /locus_tag="HJ591_RS28545"
                     /old_locus_tag="HJ591_29295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2763..2921)
                     /locus_tag="HJ591_RS29075"
     CDS             complement(2763..2921)
                     /locus_tag="HJ591_RS29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3561..3737
                     /locus_tag="HJ591_RS28550"
                     /old_locus_tag="HJ591_29300"
     CDS             3561..3737
                     /locus_tag="HJ591_RS28550"
                     /old_locus_tag="HJ591_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3727..4071
                     /locus_tag="HJ591_RS28555"
                     /old_locus_tag="HJ591_29305"
     CDS             3727..4071
                     /locus_tag="HJ591_RS28555"
                     /old_locus_tag="HJ591_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4280..>4714
                     /locus_tag="HJ591_RS28965"
                     /pseudo=""
     CDS             4280..>4714
                     /locus_tag="HJ591_RS28965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4749..5258
                     /locus_tag="HJ591_RS28565"
                     /old_locus_tag="HJ591_29315"
     CDS             4749..5258
                     /locus_tag="HJ591_RS28565"
                     /old_locus_tag="HJ591_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            5265..5477
                     /locus_tag="HJ591_RS28570"
                     /old_locus_tag="HJ591_29320"
     CDS             5265..5477
                     /locus_tag="HJ591_RS28570"
                     /old_locus_tag="HJ591_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3396..3475
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2813..3210)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4062..5593
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgcaggaa aacagggttc tcatcgggca tttacgctga aaccggatgt cattcacctg
       61 gctgcatggc tatgcagcca ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt
      121 ggtttgttgc cggttttacc tgactgccac cccgttcgcg gcgaacccgt ccggggcggt
      181 gcgggcaacg ggtgttatgt ttaatcagcg tgaacggcag atacaaaaaa gccccggagg
      241 gcttttaact actgacttat ttttttgaag atttgctggg tttcaatttg tcagtatttc
      301 ccggcgtttt tttattgtca cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc
      361 gtttcggacc cggcttaaga cccataatcg ttatcctttc aatagttaag aggaaagccc
      421 tcaagctaga tatattgacc gcgttacgga ttatcaagag gggctaacaa gattattttt
      481 tgttctttta ggttgattgt ggtactgttg atatacatgt atagcaaatg gtgttcggga
      541 ggtttatatg cttgctatcc ggttatctga tgagattgag tcccgtctgg actcgctggc
      601 gaagcaaacc ggcagaacaa agacgtttta tgcgcgggaa gcaatactgg cgcatctgga
      661 agacctggag gattattatc tttcagcaga aactgctgca cgcgttcgcc gtggtgatga
      721 agcagtgcat tcgtctgaag acgtgaggaa gtcacttggt ctggacgatt aactattccg
      781 atcgggcgct caaatcgtta cgcaagatgg acaaacagaa cgcacgacgg attgtggatt
      841 ttatgagttt acgcattgca gttgctgccg atcctcgcca gtcagggaag ccgctcaaag
      901 gtgagctggg cgagttctgg cgctatcggg tgggagatta tcgcgttctg tgtgagatcc
      961 gagatgacga gcttgttatc cttgccgcca cgattggaca tcgccgcgaa gtttacgact
     1021 gaagcccgct atgcgggctt ttttgatgcc gacaacaggt attatggtaa atactcgact
     1081 aactacgtgt tctactgttt gtcggtttta gttaaccagc aggttgtctt aacgataatg
     1141 tagccgttat ttttaactaa taaggtcata ttgttaacgt caggcataat aaaagacatt
     1201 atggcctcag agttatcatc agaagcagct ctctgcatta ctacttcgat tcccttttct
     1261 ttgaatctat aagtatccag ttgcctatta aacttagaaa gacctacaac catacttagt
     1321 ttgttatctt taacagtaag agatgcttcc tgagtaccat tacatcggta gtaagcggtg
     1381 taaccgtcag caaaagatcg accggggaat ataaatgcta ggaacgcaat taaccaatac
     1441 atagttgaga atcttggtat tacacatttc actatcctct ctttcatttt tcgttcacca
     1501 tactttgtta tctgattttt aatttttcta ttttataaac caagtctcta ccaaaaaggc
     1561 gaaaattatc ctcaaataat aataattgtt taagaacatc catgaaagca gctgcttcac
     1621 cctcagttat gggagctact ctatataaca tataacctga gttaagggtt tcttttatac
     1681 tttcttcaat gctcattata aatattagtg gattatcatt tttcttcccc catgggtttc
     1741 taattaatcc ttcgatgtac tcaggtcctt tgcgtcctat tgcctgcgca atatttttta
     1801 tttcatctgc gtgagggctt tgttgtacag ctttgtttat gcctgcttga gtgattttca
     1861 gagactctac accgtgtatg ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa
     1921 ttaatccatt tttgtttaca tctgagtatt tggataaaaa acgtaatgaa gtgttaactc
     1981 tcccttgaag cctaacaatt gggagtgctg ccttgtatat ttccttctcc gttaaatacc
     2041 gtttaaaaaa cataattctc cgcctccgta tagcgtgcct attttatttc gtcgtaaaac
     2101 ttagctctga aatattcctt tagaaccatc atgtcattaa taattgaatc tatctcatcc
     2161 attttttctt ggtattcttt taatggtgaa gggagcggta actcaatatt tgaaaagtga
     2221 ttggcgagat agggatcaag gtttctcatc cattcaggct tttcgccaat gcgttcttca
     2281 actgaaagtg aacctgataa tctctggtta ataagatcac ttagactcat tttggtttta
     2341 ttcgaaagcc gttgtaagga ttgctttaat cctatatcta gcatgatatt tgttctttct
     2401 ctcattaccc ccccttgcta ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta
     2461 agaaacgcaa taaaaaagga gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg
     2521 gcatcagtgg cgggtgtttt tccgtgggat ggatggagct ccggggataa cagaactgac
     2581 gatcatttaa aggatcttct tgagatcctt tttttctgcg cgtaatctct tgcactgtaa
     2641 acgaaaaaac cgcctgggga ggcggtttga tcgaaggtta agtcagttgg ggaactgctt
     2701 aaccgggtaa ctgggtatac gagaccgcag ataccaaaac tgttctttca gtgtagccgc
     2761 agttaggcac ccacttcaag acctctcaat atctctctcg tatatatcca gttaccaatg
     2821 gctgctgcca gtggcgtttt tgcgtgtccg tccgggttgg actcaagatg atagttaccg
     2881 gaaagggcgc agcagtcggg ctgaacgggg ggttcgtgca tacagcccag cttggagcga
     2941 acgacctaaa ccgagccgag ataccagcag cgtgagctat gagaaagcgc cacgcttccc
     3001 gaaagggaaa aaggcggaac aggtatccgg taaacggcag ggtcggaaca ggagagcgca
     3061 cgagggagcc gccggagaga aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc
     3121 tctgacttga gcgtccattt ctgtgatgct cgtcagggga ggcggagcct atggaaaaac
     3181 ggcaacgccg catccttttc tccggccctt tctgatttga gcgcctttgc tttatcccgt
     3241 tatccgtgag tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct accgagtgag
     3301 cgaggaggcg tcatttcatc tgtccgtgca tggtattgcg gacgctcctg agccacatat
     3361 cacacctgcc acatgaggca ctttcatgaa tcgtgctctc atgccaacat agtaagccag
     3421 tatacacagc gtaaacgctg tgactggttc agggctgcgc cccgaaaccc gctaacacca
     3481 ctgacgcgct ggcgcttgtc caacgccgga gcgacccgga aaacttttcc gtgccgtccg
     3541 gcgtttgcga aggagggata atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa
     3601 gatcctggca tccggcagtg atggcaaatc tgatgcgtca ggcacaggag gaagattacc
     3661 gccgggagca gatggcagag cttgagcgtt cagcccggtc acattttgcg gatatcggag
     3721 gagaggatgg cggttaagcg tacccgtttt ttaggtatcc gggtgaccga tggggagtac
     3781 cagcagctgc tggagcgctg caatggaagg caactggcgg tgtggatgcg ggaaacctgt
     3841 ctcgatacgc gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc
     3901 cagcttgccg ggatggggaa caacctcaac cagatcgccc ggaaaattaa cggcggccag
     3961 tggtcaggcg ctgaccgggt tcaggtggtg gccgcgctga tggccattga tgccgaactc
     4021 gagcggctgc ggcatgccgt gctggaaaag ggggcagacg atgatcgtta agtttcatcc
     4081 gcgagggcga ggcggcggtg ccgggccggt ggattatctg ctggggaaag accgccagcg
     4141 cgacggggcc atcgtcctgc agggaaagcc ggaggaggtc cgggagctta tcgacgcctc
     4201 gccctacgcc aaaaagtaca cctccggcgt cctgtctttt gctgaacagg atttaccgcc
     4261 cggccagcgt gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg gactcgataa
     4321 agaccagtac agcgtgctgt gggttgaaca ccgggacaag gggcggctgg aactgaattt
     4381 tctggtaccg aacacggaac tgctgaccgg caggcgtctc cagccgtact acgaccgggc
     4441 agaccgtccg cgcatcgatg cgtggcagac cgtggtgaac gggcgactgg ggctgcacga
     4501 cccgaacgca ccggagaacc ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa
     4561 gcaggaagcc gcagaggcta ttacgcgggg cttactggcc ctcgcctcgt caggggagct
     4621 taaaacgcgt caggacgtca ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac
     4681 cacaaagagc agcatcagca ttgccgaccc ggacggaggg cgaaacatcc gacttaaggg
     4741 agccatctat gaacagtctt ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc
     4801 agcagcagag taccggcgaa atgctgaaag ccgcattcag cgagcacgag aagtctgtca
     4861 gagcggaact gagcgaaagc gggaagagaa tcagcgccgc catccgcgcc cacgagcagg
     4921 gtatgagcgc ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat
     4981 ggctgaccat cacgatggtg tcaggactgc tgttcgccag cctgagcggg gtgttgtggt
     5041 atcaggggag cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt
     5101 cgaagctgaa cgcgaagacc tggggcgtga cgtacctgga agacagcaac ggacgttttc
     5161 tggtgctgcc gaaggggacg gccgtagaca ggacgcagag ctggacggtc gggaacgggc
     5221 ggtcgaagca gaacgcgctg agactggtga aggagtaagt gccgatgacc gagctggaaa
     5281 aacagttgct gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt
     5341 gggagagcgc cttcggggaa tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg
     5401 cgctgagcga gcgcgtgacg cgcttgagtc agcaggtgga acgcttgagc gggcaactgc
     5461 agcgtttgag ccggtagtgc agcgtcatga gatggccgtg gccgcagaac gggcgcacga
     5521 gcaacggcag cacgaaaagg aactgacgga agcgcgttcc cggcagcgaa gctatgacgg
     5581 tccgtcgctg ggctga
//
