LOCUS       NZ_CP053019            10060 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN361 plasmid pKPN361-3, complete
            sequence.
ACCESSION   NZ_CP053019
VERSION     NZ_CP053019.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14752225
            Assembly: GCF_016804025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Du,N. and Du,Y.
  TITLE     Genome sequencing and assembly of Klebsiella pneumoniae strain
            KPN361
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Du,N. and Du,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2020) Clinical Laboratory, The First Affiliated
            Hospital of Kunming Medical University, Xichang Road, Wuhua
            District, Kunming, Yunnan 650032, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 872.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:54:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,758
            CDSs (total)                      :: 5,639
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 55 of 151
            Pseudo Genes (incomplete)         :: 102 of 151
            Pseudo Genes (internal stop)      :: 21 of 151
            Pseudo Genes (multiple problems)  :: 23 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP053019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN361"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN361-3"
                     /geo_loc_name="China:Yunnan"
                     /collection_date="2016-10-03"
                     /collected_by="The First Affiliated Hospital of Kunming
                     Medical University"
     gene            join(9718..10060,1..23)
                     /locus_tag="HJ591_RS28875"
     CDS             join(9718..10060,1..23)
                     /locus_tag="HJ591_RS28875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            198..476
                     /locus_tag="HJ591_RS28460"
                     /old_locus_tag="HJ591_29210"
     CDS             198..476
                     /locus_tag="HJ591_RS28460"
                     /old_locus_tag="HJ591_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            464..778
                     /locus_tag="HJ591_RS28465"
                     /old_locus_tag="HJ591_29215"
     CDS             464..778
                     /locus_tag="HJ591_RS28465"
                     /old_locus_tag="HJ591_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            942..1370
                     /locus_tag="HJ591_RS28470"
                     /old_locus_tag="HJ591_29220"
     CDS             942..1370
                     /locus_tag="HJ591_RS28470"
                     /old_locus_tag="HJ591_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1396..1575
                     /locus_tag="HJ591_RS28475"
                     /old_locus_tag="HJ591_29225"
     CDS             1396..1575
                     /locus_tag="HJ591_RS28475"
                     /old_locus_tag="HJ591_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1602..2132)
                     /locus_tag="HJ591_RS28480"
                     /old_locus_tag="HJ591_29230"
     CDS             complement(1602..2132)
                     /locus_tag="HJ591_RS28480"
                     /old_locus_tag="HJ591_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2139..2870)
                     /gene="mobC"
                     /locus_tag="HJ591_RS28485"
                     /old_locus_tag="HJ591_29235"
     CDS             complement(2139..2870)
                     /gene="mobC"
                     /locus_tag="HJ591_RS28485"
                     /old_locus_tag="HJ591_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2870..4834)
                     /locus_tag="HJ591_RS28490"
                     /old_locus_tag="HJ591_29240"
     CDS             complement(2870..4834)
                     /locus_tag="HJ591_RS28490"
                     /old_locus_tag="HJ591_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6318..6467)
                     /locus_tag="HJ591_RS28495"
                     /old_locus_tag="HJ591_29245"
     CDS             complement(6318..6467)
                     /locus_tag="HJ591_RS28495"
                     /old_locus_tag="HJ591_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6552..6809)
                     /locus_tag="HJ591_RS28500"
                     /old_locus_tag="HJ591_29250"
     CDS             complement(6552..6809)
                     /locus_tag="HJ591_RS28500"
                     /old_locus_tag="HJ591_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6819..8504)
                     /locus_tag="HJ591_RS28505"
                     /old_locus_tag="HJ591_29255"
     CDS             complement(6819..8504)
                     /locus_tag="HJ591_RS28505"
                     /old_locus_tag="HJ591_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8831..9076
                     /locus_tag="HJ591_RS28510"
                     /old_locus_tag="HJ591_29260"
     CDS             8831..9076
                     /locus_tag="HJ591_RS28510"
                     /old_locus_tag="HJ591_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9350..9721
                     /gene="tolA"
                     /locus_tag="HJ591_RS28515"
                     /old_locus_tag="HJ591_29265"
     CDS             9350..9721
                     /gene="tolA"
                     /locus_tag="HJ591_RS28515"
                     /old_locus_tag="HJ591_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     BGC             1819..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5637..6215
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2143..2870)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgatgatgg ttgccgccag taacaggtcc tttaacccgc aggctgcaat agtcctggat
       61 gatgagtgag cctgtagcag ccctgattag tggaagcgaa gctgaaaaat gggcagcaaa
      121 agccgtattt ctggcaaata gcgccgccac ggattaaagt agtcatgtgt aatacatatg
      181 actacaaagg ggcttttatg tcatcagcaa catccatcag gctcgatgaa gaacttaagg
      241 accggctgaa aacgctggcc gatgaccggc accgctcagc gcacgcgcta atgctggagg
      301 caatcaccga atacatcgac cgggaagaga agcgcagcca gtacctgcgg gacggacagg
      361 ccgcatggca gcactatcag gaaacgggtc tgcaccttac tgcggaagaa gcggaagcct
      421 ggataagtac ctggggaact gagaacgagc aggatgctcc gccatgccac aggtaacgat
      481 ttccgcgctg gcacagcgcg atttacaacg ccttcaggac tttctcaaaa ccaaaaatcg
      541 gctggcagcc agaaaaggag gtgaggtgat cgtccgggct atccagcaac tgaaaacatt
      601 gccagacatt ggccgcccgg tgccgtttct gccgctggaa tataaggaac tggtgatcgg
      661 gtttggcgac agtggctatg tgatgctcta ccgccacgac cgggaaatgg accagattgt
      721 gatcgtcacc gtcaggcatc agaaagaatc cgggtatccg ggggcagaca gcctctaata
      781 accgatgacg ggggcgcaat tcattgcgtg gccgtcgccg gtttgacaag cgcagcgcgt
      841 cagaaaacca catcctgaac ggaaaaacgg gtaaaattca gcgatacatt taccgtgaga
      901 aattacacac ctttcacacc cgataaaatc aggggtatac agtggcgaaa acattgctca
      961 acctttcaca ggcagcacaa gccgcgggca ttaccagaag aacgctatac aatcatgtaa
     1021 aacagggaaa ggttaccgtg tcacgtgacg ggaaaaacaa ccctgtcgtg gatgtgtcag
     1081 aactgatccg tgtatacgga aatgtgaata tccctgagaa acaaattccc ggcatttcac
     1141 accgtgaaaa tacacaaaaa aatttcccac aggaacagct tctggcaatg caaaaggaac
     1201 tggccgatct ccggcaggct gtcaccctga tgctggaaga taaaacatcc cgggaagaag
     1261 aacgccgcca gcacgatgat gagcgccgga aactccaggc cgaagtggac agattgacaa
     1321 cagaactgac acagaagaaa aaacgcttct ggtccggctg gtttagctga gaacttaccg
     1381 aagggaatgg agaacatgac gaagcaggaa aaaactgccc tcaatatggc taaattcatt
     1441 aaggatcagt cgctgctgtt gctggaaaag ctgaacgagc tggacatgga taccccggcc
     1501 gacatgtgcg aacggcttca cgaggatgcg gaaacactct ggcaggtgat gcgggataag
     1561 ctcggggaag aataacagca ccggccatgc cggtgctggc gttatttttt gccgaacggc
     1621 caccaccggc gggcgggttt gtcctccggc agttcaggca ccggcacgtt aaaatagcgg
     1681 ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct ttccccgtgc
     1741 cgctcgctga ccacctcaat catcgcgtta atctgctccg gtgagcactt cccggcatca
     1801 accacctgca caccgttctc gatggcgaac cgctccacct ttcgatagag gtaatcacac
     1861 agcttcattc ctgtgggttt gtcccgttcc cattgcggat tttcaccggt gaaaaagtac
     1921 gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt catcatcgcc
     1981 agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca ggcgagcacc
     2041 accatgtctt ccagatgctc gctggttggc ataatgcagc gctcgccgtc tttgtcttcc
     2101 acgtcctcaa taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta
     2161 aagcacgcga acagcctctc ccggtccagc tccaccgttt tgcgggcctc gccggtctgt
     2221 ggattcacga tttcatccac cgccagaatc gcccgctgta cccggacaag cgtcggccgg
     2281 tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa cagatacccg
     2341 gacaccactt cctgataccg cctgcggctc ttgacggtgc gctctacctc aaatgccacc
     2401 gttctgccgt ccagctccgc cagcgcgtcc ggcactttca tccctttcag ggccatacgg
     2461 tgcaggtacc gccagccggt ggccccccgg ccctccatgg agaggcgcag ggactgcacg
     2521 gccagccggt gcggcagctg cgccgccgac acccttgacg gctgaaacgg aataacgtcc
     2581 gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag cccccacacg
     2641 ctgacctgac ggccgtcaac cgggtatttc gcccggacca gcagcccttt cgccaccaga
     2701 ctgtccagga agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg cgcaaccagc
     2761 tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt tttctccgcg
     2821 ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat cagtaaagcc
     2881 ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc cggaggcagg tactccgcca
     2941 gcgggtccgt tttcgccagc accgggaagg ttttcagcga aatttcccgg cgctcaaccc
     3001 gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc gcgaacccgt
     3061 caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc
     3121 tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg cgctccacgt
     3181 ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg ctggccgcgc
     3241 gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa tcagcgggca
     3301 gatcctgccc gcactgaccg aagtcgccca gcgactggtg cgcgagcagc aggttgcagt
     3361 cccggctgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcaga tatttaatct
     3421 catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca atgatgattt
     3481 gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg tccatcgagc
     3541 cgatgacgta caggcagccg ccgccgttga ttatccccgc cacatcgata ccgctgtccg
     3601 tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc ttcacgtcct
     3661 ttttcagggc ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg tttgccccct
     3721 gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg
     3781 ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc agggcgtact
     3841 ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg
     3901 tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct ttggggtcga
     3961 agtacaccac agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatcg
     4021 cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc caggtatcac
     4081 gagggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc tgctgtttcc
     4141 gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc ccgaaatgca
     4201 ggtggcggac gtctgtcatt tctccggtcc gctggctgct gcgccggctc tcctgattca
     4261 gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc atcaagcagc aggaacgcca
     4321 gcagccgcgc cagaaggacg ccggcgacaa gccctccggc acaccaggct atcccgaaca
     4381 ggttagccgg ctgcagcagg taataatctg cgttcgcata gaggtccggg cgcaggacgg
     4441 tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg
     4501 cagcgccggt cagccagtac gcgtagttcc agacatgtcg ccgtgccctg accatccgcc
     4561 cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt ccggttatta tcgcgcttat
     4621 agcgatcggc atactcccgc cgcgccttat ggctcagccc cacaaaacgc attcggccgg
     4681 cccagagttc agccagaccg aaaaacaggc cgataaccag aaaaaaaccg gccagactgt
     4741 aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc tccgggatat
     4801 gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc agacgacggg
     4861 tttaccggcg tgatgcgcag cccccgctgc acaagccgcg gacatgcccc aaggcatggc
     4921 ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc agcctctttc
     4981 cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca gcgtgtgaga
     5041 ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc
     5101 atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg tcaggtgcgt
     5161 ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc gggtgaattt
     5221 ctggcgtggc cggcgtccag ccggacagag ccagaaagag gggaaaagac cgtgcgtcgg
     5281 tggcgtccag ccagcgtaag caaggtcttt tcccctgaag tcagccacgc cccagcgggg
     5341 atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac gtaaaatgtt
     5401 cagcgaaaaa ccgacatggt tcacctatcc tgataattga tcgtcaggga atagaaagac
     5461 ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca ggtgccgggg
     5521 aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc
     5581 tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag
     5641 gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt
     5701 tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca accatgaatc
     5761 tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg gtaggtcgct
     5821 ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc gccgttctcc
     5881 cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc tcggtgtagg
     5941 tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac tgctgcgcct
     6001 tatccggtaa ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc cactggcagc
     6061 agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg
     6121 aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa
     6181 agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc
     6241 ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga
     6301 agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt
     6361 gccaccgttc cgccctgaac atcccggata taatttgcct gacatgcggc cagaagaaag
     6421 ccagcaacaa tcaatataaa aagaaaaata gtctttacct ttttcatata ctttccctgt
     6481 atttaaagac tccggcattc atccctgaac accggaagtc agtatttcaa atcatccttg
     6541 ataatttttt gttaccagtt accgtcccga taatcaaaag ctatataaca attatccaca
     6601 tcaggtttta tttgattttt aaaaaatggc tgtagtaaag gaacccattc tttcttcaaa
     6661 tcaaactcac cattattaat attatccttt aaaggcattc ccaagctttc aatgacagaa
     6721 ccatcgtcac caaagtcttt agaatactct tcccctttaa actcttcagt tttcttatca
     6781 aaccatgtca aacgtaattt aagccccata tttacctctc aaagatattt tttaatgttc
     6841 cgttttggat ctggcccttt aacctgctta cccgttttgg gatcaaatgc gcctaggtgt
     6901 tcgccatcac tggcacgata cccttcaagc tcaccgtgct gggaatccca ttcataaatt
     6961 ttacgttttt tatcgccata ccatcgggcc cgcttaccac caccaccttg tttaggtgtt
     7021 ttaggatcac ctcgctttaa ctctccaagt ccctttatgt cttctgtttt aggtactgga
     7081 tgataatcat ggccgtagtc tttagtcccc ttgcgaggct tgttcttttc ctcatttaac
     7141 ttattttctg catccttcgc ttttttctcc ttttgtttcc ggctctccat cgccccggta
     7201 agtgcagcct tatctctgga taaggattca ttcattgcgt ttaaagatgc ctgagccgct
     7261 tttaatttct cattctgatt ctgaatatcc ttatcaagat agcgaacctg atcctgccac
     7321 ccggagccat attcatgagg cgggttataa gtcacaaaat catctttatt tgcctctgcc
     7381 tcttttacct ttttatcggc ttcctgaaca gcagcccttg caccggggag cgccttctct
     7441 gtcgatgcaa tgcggctgtt taaggtcgca atatttttat cttcggcatc cagctcagct
     7501 ttcgctttat catagtctct tttcagcccc tcttccggat gagcggcgtc ccatgcctgc
     7561 tggcgacgct tttcttcctc ctgacgctgt tttacctgtg ccggggtaag aacatctgtc
     7621 accgacacat aaaccggctt ctgtccggtc tctttcggga aacgaataac ggcctcacgg
     7681 gagttaccac cggctgtaaa accagccgga cgtgaatcac ctttttcagc aacaatgcct
     7741 tttggcgggg ctttcgctgt cggaacacct ttcggtacgc tcacctgcag agacgggaga
     7801 cccggaatcg acacactgaa tacccccgga cgttttgtcg gtttcgcatc caccacaggg
     7861 acactcattg gccgacctgc gacaaccgca atatgctgcc gctcatcctt caccacatcc
     7921 acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc
     7981 tccgtcaccg catcggccgg taatgacgtc ataatttttg acatcatttt cggatcatct
     8041 tttgcaatct cagaaggcag cacaccgtag atcgcaatcc cccacagacc aaacttaaac
     8101 ggccccttca gggcagccag cacatcagca acggcggccg acaacgcatc gccggaaact
     8161 gataaagcca gtccttcagc ccccggagta gccagggcag gcagaccgaa ggccatcgca
     8221 gtggccgaag actgtccgcc accagagcta ccgctatgat tgccgctgtt accaccatta
     8281 ccaccgggtt tactcccgcc gttaccaaac tcccccggat tatagttatg aatatctcca
     8341 ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc accacctgat
     8401 gaagggccac ctttaccaga cgtaccgttc acattcccac tggcctgacc accattatgt
     8461 cccagacctg aattacccgg acctcgaccg tctccaccac tcataccgtt ttcctctttg
     8521 acatttaaaa caataaatta aagccaacat actgtacata tacacagtat aattattacc
     8581 ttactcctcc cgttttcagg tgtcaatata tgagattagt tctcatcttc aggcacaaaa
     8641 aagcgcccgg agccacctgt aagcagatcg cgtcttttcc cgaaaatttc tcatggcaac
     8701 aaattttatt atttaagaca ttcatcacac ttacatttca gatggtatag acacatctga
     8761 aagctaactg gcatgaggag aggacctgcg gttaaactgt tcgggaaacc tgagacgccc
     8821 ggaggccact atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc
     8881 ctttgtcgct caggcactgg ccatgggcct gaaagggaac aaaacgctgc caaaggcggt
     8941 aaaacaacag gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg aagccaggcg
     9001 ggcgcaggaa atgcgtcggg agaaatacga ggctacggaa accaccttca cctggcagcc
     9061 acagcgccgc cggtaagggg tatttaccat aacgtcgcat tgcccgcacc gctcagacgg
     9121 tctgagggcg tttccggcgg ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc
     9181 cggaaacgcg cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg
     9241 gccgtttggt gtaaaaacga tacgataagc cgtgtttatg taaatgaacg tggccgtaaa
     9301 gagtgacaaa tatacatgta acatttttta ctgacaggtc atcaacccga tgcgtttaat
     9361 accctttatc ctgctggcat tgtgcagccc cttactcctg acggcctgta agcattccac
     9421 cacccagccg ccgaaaattt atactctgga taagtcatct aaaatcagta gctacgctgc
     9481 ggccatcaag accgctgttg aaatgcagat gtttgaacca gaggcctatg cgggaaaaat
     9541 gtgcaccgtg cggatatcca tacaacgtga tggcactctg aacagcgcca ttgctgaagg
     9601 aggagatccg aaactctgta aagcagcaat atcagccgtt acccacgcca aaattccgcc
     9661 ggcaccggat gaaaatacct ggcaaatatt caaaaacgcc cctctggatt tcagaccatg
     9721 aaagctttta ctctgacagt gctggcggcc atgttcctca ctccggtaca tgcgatacca
     9781 ctaaaacccg taaccacact gcagccaacc tacccggtac aggccgcagc aaaacgcatc
     9841 gaaggtcagg tgaccgccac gtttgatatt ctggacgatg gacgagtgga caacgtgtcg
     9901 cttaccggct gtcacctttt ttttcgggaa acgctgacgg caatgaggca ctggctatat
     9961 cccgcagggc acccggcaat gcgacaggtt ataaattttg aattcagtct gaaaaatgac
    10021 ggcttacagg atccgcgacc agtcaccacc caaaagctca
//
