LOCUS       NZ_CP051850             7472 bp    DNA     linear   CON 10-JAN-2024
DEFINITION  Escherichia coli strain SCU-101 plasmid pSCU-101-1, complete
            sequence.
ACCESSION   NZ_CP051850
VERSION     NZ_CP051850.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609767
            Assembly: GCF_017815815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Hartman,A., Gonzalez,A.,
            Gill,M., Pandori,M. and Hess,D.
  TITLE     F Plasmids Are the Major Carriers of Antibiotic Resistance Genes in
            Human-Associated Commensal Escherichia coli
  JOURNAL   mSphere 5 (4), e00709-20 (2020)
   PUBMED   32759337
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 02:10:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,245
            CDSs (total)                      :: 5,129
            Genes (coding)                    :: 4,868
            CDSs (with protein)               :: 4,868
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 165 of 261
            Pseudo Genes (internal stop)      :: 60 of 261
            Pseudo Genes (multiple problems)  :: 74 of 261
            Pseudo Genes (short protein)      :: 5 of 261
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051850.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-101"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-101-1"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            289..726
                     /locus_tag="HHJ20_RS25855"
                     /old_locus_tag="HHJ20_25840"
     CDS             289..726
                     /locus_tag="HHJ20_RS25855"
                     /old_locus_tag="HHJ20_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            807..1235
                     /locus_tag="HHJ20_RS25860"
                     /old_locus_tag="HHJ20_25845"
     CDS             807..1235
                     /locus_tag="HHJ20_RS25860"
                     /old_locus_tag="HHJ20_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1488..1763)
                     /locus_tag="HHJ20_RS25865"
                     /old_locus_tag="HHJ20_25850"
     CDS             complement(1488..1763)
                     /locus_tag="HHJ20_RS25865"
                     /old_locus_tag="HHJ20_25850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001555311.1"
                     /translation="MSYLWSVSASRKYRNGHWMALRWDGNQRQAKMLSARFRCIFWDNA
                     WFGAPGTRFSQTLLKTQERVKTHTGAILVCLCCCVFAFIARSRSFP"
                     /name="hypothetical protein"
     gene            complement(1794..2027)
                     /locus_tag="HHJ20_RS25870"
                     /old_locus_tag="HHJ20_25855"
     CDS             complement(1794..2027)
                     /locus_tag="HHJ20_RS25870"
                     /old_locus_tag="HHJ20_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2034..2552)
                     /locus_tag="HHJ20_RS25875"
                     /old_locus_tag="HHJ20_25860"
     CDS             complement(2034..2552)
                     /locus_tag="HHJ20_RS25875"
                     /old_locus_tag="HHJ20_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_137596602.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRRLGESVSEAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTASG
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2590..3288)
                     /locus_tag="HHJ20_RS26605"
                     /old_locus_tag="HHJ20_25865"
                     /pseudo=""
     CDS             complement(<2590..3288)
                     /locus_tag="HHJ20_RS26605"
                     /old_locus_tag="HHJ20_25865"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3230..3553)
                     /locus_tag="HHJ20_RS25885"
                     /old_locus_tag="HHJ20_25870"
     CDS             complement(3230..3553)
                     /locus_tag="HHJ20_RS25885"
                     /old_locus_tag="HHJ20_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3618..3809
                     /locus_tag="HHJ20_RS25890"
                     /old_locus_tag="HHJ20_25875"
     CDS             3618..3809
                     /locus_tag="HHJ20_RS25890"
                     /old_locus_tag="HHJ20_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3961..4179)
                     /locus_tag="HHJ20_RS26610"
     CDS             complement(3961..4179)
                     /locus_tag="HHJ20_RS26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072021258.1"
                     /translation="MFFPALSPDSVDNRITAFECAGTARRSRTTERSESVSEEAEECRK
                     LHFLLTHLCGISHRLWCTFHTIRTDAA"
                     /name="hypothetical protein"
     gene            4479..4637
                     /locus_tag="HHJ20_RS26615"
     CDS             4479..4637
                     /locus_tag="HHJ20_RS26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4834..4974)
                     /gene="crl"
                     /locus_tag="HHJ20_RS25895"
                     /old_locus_tag="HHJ20_25885"
     CDS             complement(4834..4974)
                     /gene="crl"
                     /locus_tag="HHJ20_RS25895"
                     /old_locus_tag="HHJ20_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            5094..5618
                     /gene="cui"
                     /locus_tag="HHJ20_RS25900"
                     /old_locus_tag="HHJ20_25890"
     CDS             5094..5618
                     /gene="cui"
                     /locus_tag="HHJ20_RS25900"
                     /old_locus_tag="HHJ20_25890"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            complement(5646..>7472)
                     /gene="cya"
                     /locus_tag="HHJ20_RS25905"
                     /old_locus_tag="HHJ20_25895"
     CDS             complement(5646..>7472)
                     /gene="cya"
                     /locus_tag="HHJ20_RS25905"
                     /old_locus_tag="HHJ20_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_209524095.1"
                     /translation="DGTGWSSESGGPAPGGGMHGNSGGQRGDNANSSNSVSQQISAIQN
                     DQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVTIEGITSTQAKQLGLGGLVMGY
                     NASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNGQKPAGEWHAVAKDSWTGAGPV
                     NVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAMRQAFDSLPLAKQGEAVRQIVA
                     AWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQISSGMSASQKDVDAANRIINETV
                     KAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVFKNHSYHRIRDAQRAYDDARNK
                     NDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKADAKAKAEAEKAAAEAKAKAEAE
                     KARKEAEAKANNEKAVLTKASEIIISVGDKVGEYLGDKYKALSREIAGNIKNFQGKTIR
                     SYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAEDMGNKFAALGKTFKAADYVMK
                     ANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVALAIFSATLGAYLLAVGASAAVV
                     GIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     Typing          complement(3895..3982)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          4005..4551
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1691..3240)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.176"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accataccca ccgtaattaa accccggcat attcattcct ctttgacttt taaaacaaac
       61 cattaaaaac aaatactgta tacataacca ctggttttat gtacagtaca aaagctacgc
      121 cgctgcattt tccctgtcaa gactgtggac ttctattttt gtgaaaacga tcaaaaaaac
      181 agtctctcac accacgcgct attcccgccg gataccacaa aaaccggcgc aaacattacc
      241 gttctcagac ctcattatgt tttactgaaa ccatgagatg agacacctat gggacactgt
      301 aactttatgg catggcacac actccgggac gcactgaaaa tgactggaag atcgcgttca
      361 cagttttacc gtgatatgcg ctcaggtctt gtcagttacc gtaccagcca ggacggacga
      421 agggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca aaatgagaca
      481 caagaaaggc acactgtggg acaggcagaa aatccacatg atcagcagac agaacgcatt
      541 ctccgggaac tgaatgagct gaaacaatgc ctgacgctga tgcttgagga taaacaggca
      601 caggatatgg atcgcaggca ccaggaatca gaacgggaac agttacagaa tgagatagcc
      661 cagctcaggc aggcactgga acaggaaaag aaacggggat tctggtccag actcttcggt
      721 cactgaactc ggtcagaggc tgatgataag atagtattcg gataataact caccgagaat
      781 aaatacttta aggtaaggag acacgtatga aacgttctgg tggcacacgc tatctgtatc
      841 ttatatccct gataactgtg gctgcagccc tcacggcctg taccccgaaa ggcagtgtgg
      901 aacaatatac ccgacattat gtgtacgctt ccgatgaccg ttctgaccct aacttctaca
      961 ccaacaaggc cgacaccaca cggatgatga ttccgttctt tcagcagttc agggagatgg
     1021 gggagaaaga caaagcagcc ggagtatcag cagaaacggc acagcaacgc ataaaagaat
     1081 tccactcaga aaaatttctt cagtctctca gaagcacaac cacctttgcg ggcagaaaat
     1141 atacgaacag tgatatgcca tcaccggaaa aaatgaagct actggctgac acgatttctg
     1201 cggtttatct cgatggctac gagggaagac agtaaatgga tttaccataa tcccttaatt
     1261 gtacgcaccg ctgaaacgcg ttcggtgcga tcacggcagc agacaggtaa aaatggcaac
     1321 aaaccacccg aaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat gcatagctat
     1381 gcagccatgt gaatcacgca ggaattgcca gccagagact gctgaaactg atttttttca
     1441 tactttagga agcgaagaag acgagactgg agcggaaaaa cagaagttca ggggaatgag
     1501 cgcgatctgg caataaaggc aaaaacacag caacaaagac acaccagaat cgcgcccgta
     1561 tgcgttttaa cgcgttcctg tgttttcagc aaggtctgac tgaagcgcgt tcccggtgcc
     1621 ccgaaccagg cattatccca aaaaatgcac cggaaacgcg ctgacaacat tttagcctgt
     1681 ctctggttac catcccagcg aagggccatc cagtgtccgt tcctgtattt ccggctggcg
     1741 ctcacgctcc agagataact catgttcgcg ttcctgtatc agccgttcat cccttatctc
     1801 ctgttctcgc tgtataactg gctcaagcgt tctgtccgct cgctcaagtg ctgcacctgc
     1861 tgactcaact tcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac
     1921 attttctgca attccacgaa ggcgctctcc cattcgttca gccgctgcat atagtcctgt
     1981 tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct ccgtcactct gtgtcactcc
     2041 gtcagttgta cccagtcctt cccctgatag ggaatcacct ctgctgattt cccgtacgga
     2101 aggaccagaa atttcctgtt cccgtcctgt acgaactcca cgccccatgt cttcgcgttc
     2161 agtttctgca atgtctcttc ctgcttcctg atatcttcca ggttcgcctg tatcatcccg
     2221 ccaagatacc agagcgtccc gccactcgcg gtaaacagga aaatgaccat ccccagtaac
     2281 atcatgcccg tattccctgc cagctttaac acgtccttcc tgtgctgcat catcgcctct
     2341 ttcacccctt cccggtgttt tttcagcgat tcctctgtcg aggcggtgaa cagggctata
     2401 gcgtctttga ttttcgtctc gtttgatgtc acagcctcgc ttacagattc gccgagcctc
     2461 ctgaactcgt cgttcagcat tgtctctgta gattcggctc tctctttcag ttttttctcg
     2521 aactccgcgc ccgtctgtaa aagattgctc ataaaatgct ccttttagtc tgatattctt
     2581 cccgccgttc gggtctgcaa tactgatgct gcttcgcgtc accctgacca cttcaatccc
     2641 tgcctctgtg agcgcctgaa tcacatcctg acggcctttt atctccccgg catgataaag
     2701 agcgtctatc ccgcgtgtga cgctctcagc aagcgcctgt ttcgtttccg gcaggttatc
     2761 agggagagtc agtatccggc ggttctccgg ggcgtttggg tcatgcagcc cgtaatggtg
     2821 attaaccagc gtctgccagg catcaattct cggcctgtct gcccggtcgt agtacggctg
     2881 gaggcgtttt ccggtctgta actccatgtt cggaatgaca aaattcagct caagccgtcc
     2941 cttgtcctgg tgctccaccc acaggatgct gtactgattt ttttcgagac cgggcatcag
     3001 tacacgctca aaactcgcca tcactttttc acgtcctccc gctggcagct ccttctccgc
     3061 gaacgagaga acaccggacg tgtatttctt cgcaaatggc gtggcatcga tgagttcccg
     3121 tacttcttcc ggattaccct gaagcaccgt tgccccttcc cggttacgct cccttcccag
     3181 caggtaatca accggaccac tgccaccacc ttttcccctg gcatgaaatt taactatcat
     3241 cccgcgtccc ctgttccctg acggccagac gcagccggcg cagctcatcc ccgatggcca
     3301 tcagtgcggc cactacctga acccggtcac cggaagacca ctgtccactg ttaaccttcc
     3361 tcgctgtctg attcaggtta ttcccgatgg cggccagctg acgcagtaac ggcggtgcca
     3421 gtgtcggcag tttcccggaa cgggcaaccg gctcacccag gcagacccgc cgcatccata
     3481 ccgccagctg tttaccctca cagcgttcca gtaaacgggc atgttcatca tcagtaaccc
     3541 gtatcgtgag catcctctcg cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     3601 cttacacgga ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg gcgcgcttta
     3661 tcagaagcca gacattaacg ctgctggaaa agctcaacga actggacgcg gatgaacagg
     3721 cagacatctg tgaatcgctt cacgaccacg ccgatgagct ttaccgcagt tgcctcgcac
     3781 gtttcgggga tgacggtgaa aaccactgac acatgcagct cccggagacg tacacaactg
     3841 gtctgtgaat ggatgccgag agcagacaag cccgtcaggg cgcgtcagca ggttttagcg
     3901 ggtgtcgggg cgcagccctg acccagtcac gtagcgatag cggagtgtat actggcttaa
     3961 ctatgcggca tcagtgcgga ttgtatgaaa agtgcaccat agccgatgtg aaatgccgca
     4021 cagatgcgta aggagaaaat gcaacttccg acactcttcc gcttcctcgc tcactgactc
     4081 gctacgctcg gtcgttcggc tgcggcgagc ggtaccggcg cactcaaagg cggtaatacg
     4141 gttatccaca gaatcagggg ataaggccgg aaagaacatg tgagcaaaaa ggcaaaggcc
     4201 aacgaaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc
     4441 tcggcttggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta aggcacgcct
     4561 taacgccact ggcagcagcc actggtaacc ggattagcag agcgatgatg gcacaaacgg
     4621 tgctacagag ttcttgaagt agtggcccga ctacggctac actgaaagga cagtatttgg
     4681 tatctgcgct ctgctgaagc cagttacctt ggttaagcag ttccccaact gacctaacct
     4741 tcgatcaaac cgcctcccca ggcggttttt tcgtttacag gcagcagatt acgcgcagaa
     4801 aaaaaggatc tcaagaagat ccttttaact tttctactga accgcgatcc ccgtcagttt
     4861 agaagaggag gatggtgcga cagtccctcc ctgaacatcg cggatgtaat tagcttggca
     4921 gcctgatagc aaagccatca agacaagcat ccctcctgat aatatcattt tcatatattc
     4981 cctcattaac acatacccct aatcttagga tgaccattac ttgtacagca tgatatgctt
     5041 atacacatct acaactaaat taataataat gaaaagcttg gagagttata ccaatgagaa
     5101 aagagcacac tgacaacaaa gatttaaaca ggacgctttt tttgattact tttgcaggca
     5161 taacaccatt aatcattatt tttataactt atacatcaaa ccccaagttc tatttaatta
     5221 gcatcatttt tgataacaca caaaatatac cttcaataat ttccgcatac aatcctgtga
     5281 tgacaaaggt gatggatatc tacgggaaaa gcgctccctt actggcactt attgctttta
     5341 ctctccaact ccgcgacaga aagctagaga caatagccaa cagagaaaaa ctaattactg
     5401 ccagcatatt tagtccattt ttttatgctt tttatgcata tttctttctg tggaataatt
     5461 tcgaattaac cactgcaggt aggactgtca gatggatgtc cgataatgat tttactttgt
     5521 ttatttttta cgcctgcctc tatttttgct ctttctttat gacttatgct ctgtgttata
     5581 ttccggttgt gtcatataaa ctctggaaag aaaggtagtc ttcttgacta cctttcgact
     5641 ctaggttaat acgcagggcg aataatttcg ttatttaacc tgtctataaa tttgtcatca
     5701 atcaatgctc caataaaact ggctataata ataccgataa tccctacgac tgcagctgat
     5761 gcccccacag caagcaggta agctccaagg gtggcagaaa aaatagcaag agccacagca
     5821 gaagcaatac cactgacaac ccaggactcg acctcccgca ttaacggccc ccagtttccg
     5881 gtctgatatc cctctatact cttctcccgg acgttatttg ccttcatcac ataatctgcg
     5941 gctttaaatg tcttacccag cgcggcaaac ttattcccca tatcctctgc atcaaatgct
     6001 ttccaggcat tcacaatgac atccctgtct gcagcattaa ttttaagatc aggattagcc
     6061 atcagtttat tgacagaagc catcgcctca tcataactac ggatcgtctt accttgaaag
     6121 tttttgatat tacctgctat ctcacgagaa agagctttat atttatcgcc aagatattct
     6181 ccgaccttat cacccacact aatgataatt tcactggctt ttgtcagaac agctttctcg
     6241 ttatttgctt ttgcttcagc ttcctttctg gctttttcag cttcagcttt tgcttttgct
     6301 tcagcagccg ctttttcagc ttcagctttc gcctttgcat cagcttttgc tttttctgct
     6361 gccgccttgt tcttttctgc ctgctgtttc cttgcagtca gtccggaaac ctgtgcctgc
     6421 agcgcattaa tatcactgac cagtaaatca tttttgttcc gggcatcatc atatgctctc
     6481 tgagcatcac gaatacggtg ataagaatgg ttcttaaata ctttttttag ttcttcaacc
     6541 tctttctgtt tttgtttcat aaggcttaat aatggtacct gttgtttctt cttctccgct
     6601 attttaagat ttacgtcgtt aatggctttc acggtctcat taataatccg gttcgccgca
     6661 tcaacatcct tctgtgaggc cgacattccg gatgatatct ggttgagaat gagcgccaaa
     6721 ttaaccgcat ccaccacccg ttcagtaact cgccccatat cttttttcag atttaccggg
     6781 aaatcctgat atgccagact ccaggctgca acaatctgtc tgacagcctc tccctgtttt
     6841 gccagtggaa gggaatcgaa agcctgccgc attgctttgt actctgcttt attcatcacc
     6901 tcttcaggca agagaacacc agtcacatag ccttttttga taatccggac acttttgatg
     6961 gcgttattaa ccagccctac attcactgga ccagcccctg tccagctgtc cttcgccaca
     7021 gcatgccatt ctccggcggg tttctgtccg ttcacaaaac tctccacaga agtttcgctt
     7081 ccgggagtac ttgctcctga tgcattaagg cggtgaccgg tgtctatttc gcccacagcc
     7141 ccaataacac cagaagcgtt atatcccatg accagccctc ccaaaccaag ctgcttggcc
     7201 tgagtggagg ttatcccctc aatggtgacc tgcataacac cagaaggagc aatacttccc
     7261 agaatcattt ttgcttcagg attcattttt ctcgcagcaa tcagcatatt caccactttc
     7321 tgtttaagtt tctgatcatt ctgaatggca gaaatctgct gactcacaga attgctactg
     7381 tttgcgttat ctccgcgctg gcctccacta tttccatgca taccaccgcc tggagctgga
     7441 ccaccacttt cagaactcca gccagtaccg tc
//
