LOCUS       NZ_CP051746             6754 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-484 plasmid pSCU-484-2, complete
            sequence.
ACCESSION   NZ_CP051746
VERSION     NZ_CP051746.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609800
            Assembly: GCF_012934785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:28:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,969
            Genes (coding)                    :: 4,723
            CDSs (with protein)               :: 4,723
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 148 of 246
            Pseudo Genes (incomplete)         :: 121 of 246
            Pseudo Genes (internal stop)      :: 52 of 246
            Pseudo Genes (multiple problems)  :: 61 of 246
            Pseudo Genes (short protein)      :: 3 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051746.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-484"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-484-2"
                     /geo_loc_name="USA: California"
                     /collection_date="2018"
     gene            complement(join(6332..6754,1..1323))
                     /gene="col"
                     /locus_tag="HHJ53_RS25055"
                     /old_locus_tag="HHJ53_25065"
     CDS             complement(join(6332..6754,1..1323))
                     /gene="col"
                     /locus_tag="HHJ53_RS25055"
                     /old_locus_tag="HHJ53_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_000012960.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            complement(1556..3775)
                     /gene="mobQ"
                     /locus_tag="HHJ53_RS25060"
                     /old_locus_tag="HHJ53_25070"
     CDS             complement(1556..3775)
                     /gene="mobQ"
                     /locus_tag="HHJ53_RS25060"
                     /old_locus_tag="HHJ53_25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052986705.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMVRPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            3954..4229
                     /locus_tag="HHJ53_RS25065"
                     /old_locus_tag="HHJ53_25075"
     CDS             3954..4229
                     /locus_tag="HHJ53_RS25065"
                     /old_locus_tag="HHJ53_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(4445..5338)
                     /locus_tag="HHJ53_RS25070"
                     /old_locus_tag="HHJ53_25080"
     CDS             complement(4445..5338)
                     /locus_tag="HHJ53_RS25070"
                     /old_locus_tag="HHJ53_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(5867..6010)
                     /locus_tag="HHJ53_RS25075"
                     /old_locus_tag="HHJ53_25085"
     CDS             complement(5867..6010)
                     /locus_tag="HHJ53_RS25075"
                     /old_locus_tag="HHJ53_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(6072..6329)
                     /locus_tag="HHJ53_RS25080"
                     /old_locus_tag="HHJ53_25090"
     CDS             complement(6072..6329)
                     /locus_tag="HHJ53_RS25080"
                     /old_locus_tag="HHJ53_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     BGC             1..6323
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3418..3776
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3777..3950
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(4446..5338)
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.051"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1560..3775)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.865"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtccttttct ttattttcct tctgtttgcg gcgctcctgc gcggcactta atgcagcatc
       61 agcatctgac ttctcttttg cagcagcatc aaatgcagcc tgcttattat ttacatccgt
      121 ctgcgcccgc tgggctttaa gcccggccat ttgccacatt ctgtgaccgc cagacattgg
      181 gtcatgggca aatcgatcaa attgttttat ttcagctatt gcatcagcaa gagtcttatt
      241 cgctgcatca agttcgcttt tacgcgaatt ataaacctga acagctttag cctgtcgctc
      301 ctgatttctg gcaacatctt catttgcctg attcagctct gcacgcgcgc gttcataatt
      361 tcgctcagcc gcttcaaccg gatgcgtagc atcccattcc tgctgacggc gattttcttc
      421 atcttgacgt tgttttacct ggtcagggct aagaacatca ctcactgaaa catatacggc
      481 attatgaccg ctgtccttcg ggaatcgaat aactgcatcc ctggtattac ccccctgagt
      541 aaatcctgcc gggcgaacat ccttatcagt attatttgta acacctgggc ttaatgtctg
      601 tactgctggc gtactgttat taactgaaat attcagaaca ggtgcacctg gaattgatgc
      661 cgtaaaaaca cctggacgtt cggtaggttt tgcatcaacc accggaacac tcatcggaac
      721 acctgaaaca accgaaatat tctgtcgttc gtcttttaca tcatcaacaa cacgaacatt
      781 tacgtttact gttgccttat cgagaggtaa tgaactgaca ggtgattcag taatatcatc
      841 tgcgggtaat gacgtcacaa tctttgacat catattgggg tcatctttcg ctatttgtga
      901 tggcaataca ccatataaag ccacccccca aagaccaaat ttaaacggtc ctttcagggc
      961 agccataata tcagcaatag ctgccgataa tgctcccgct gaaatactga ccgccagacc
     1021 gccagctcct ggagtggaaa gtgccggaaa accaaatgcc actggcgcag ctactgctga
     1081 cagattaccg cctgttcccg agccaccacc ggaattacca ttccccccgc cattaccatg
     1141 accggaacca ccaccccagt gaatgccgct accggaacca ccaccccacg ggttattttc
     1201 cgaactccac ccggaaccat cagaagcacc accacctaca ccaagcccgg tcgggccacc
     1261 attaatgtta ccacttgtgc tatgcgcgcc cgtgttatgg ccgcgtccat cgccaccgct
     1321 cataaaaact ccctctttta agcgttaaaa caattaatta catgattaat actgtacata
     1381 aaaccactgg ttttatgtac agatcaaaaa gtacccgtca tcgttttttc tgtcaagttc
     1441 aggtggaaaa ttttttagaa atcaggaggg ataacactta gaaaacgcag agcgggccac
     1501 aaaaaatttt gtggcccgtg atgtgttggc ggggtccggg gtgcagcccc agatatcaca
     1561 tactcatgcc ccggaagtgc ccggggcggc gttgttgttg cctgtgctgt tcgtcccgct
     1621 tccactcatg ccaggtctga ttaaaaccga gttgacggaa gagataatcc ggcatctctt
     1681 ccggtttgtg gacagcagcc aaaagcctgt catgatggcc tttgagccgg aactgcatca
     1741 ggtgcggttc cggtgggtac ggagtatcct catcatggtt tatagcttct ccgtaacctg
     1801 ccaggcatac cccgttctcc gtgatggtgc tgactatcag atgactgtaa tccggcgacg
     1861 gaagcacaag aacggggcaa aaccctcctt cccgtcgaac aaacccgaca agcatttcct
     1921 ccggcccttt gcataacaac agattcagcg tcctgctgtt tccctctctc gaaaatacaa
     1981 caggcatgag ttgttccggt acagcatcag cggtgatatc cacccggtgc gctgacggca
     2041 gcggagaggc tacgggcctg cggtcgtcgc tgaaataaaa attatccggc cagtgatgcg
     2101 gcagcgccgg aaaaccgtaa gagggccacc cctggcgcat ctgttccagt acatgccgga
     2161 ccgtcgcacc ggaatacatt ctcacacgct caggccgcac catcgtctca tgagattctg
     2221 gcattccctc gcgtatatcc gagaccatca cagccccgcc tggcgacagg taaacattca
     2281 ccgggtcatg aacactctgg gggtaatgcg ccagctcatc accccatacc gtgaattctc
     2341 cggctctgtt actcagcatc atgtagcggg ttatcctgcc cgttcccggc tctctggcac
     2401 gccccagctc cgtaataacc ccgactaatg ccgtttcacg gctcagacgg tggttatcat
     2461 gacgtttcag ccgcgcctca tgctcctgtt ccttccggcg cgcttccgct tcggcttcct
     2521 ccctgcgccg ctccgcttcc cgtgctgaca gtttttcttt cagctctgcc attcccggcg
     2581 caatccacgt attccactgc gtttcaaagg cctgacgacg ggcgggaatg tcccgttctt
     2641 tcacggccct gtaggtgcgg cgaagagatt caatctgttt cccgagtttc cagaaacgga
     2701 gttgtctttt gcggtattcc ggttcagccc atgtcatcag gggaatctct gacagaaggg
     2761 acgcgacagg gcgtgctttt ttgagtccgg tcatctcctt ttcgagaatg gcctgctctt
     2821 tcagcagcgc attcccctca cgttcgatgt cgttcacctt cccgctcatc gccaggaact
     2881 ccggggtcat ctcccacgcg ttcccgcatt cctgcggcag cgtggcaatg aattcccgca
     2941 gggtatccgc tgaggcattc gcagcgggaa tggtcaggtt aagggcttcc gctgcacgac
     3001 gtgcagcatc gctgagttcc cgcttacgct gcgcttcctg aaccgtcctg agagcaagct
     3061 gcgtgcggga aacctgtatt tccaggttaa ggctgttaat cagacggttc tgctcaccgc
     3121 gttctgtttc cattccccgt ttttccatcg cacaggcagc ctttccgagg tgaaggcccg
     3181 gcgctttctc cagtccctga cgctcgtatg aacgatggtc tatctcttcc tggtagccgg
     3241 cgttcgcaag cgctctgttc gcatggtcag cccatgatgc gcgccacgtc tcggcgagtg
     3301 cccggtcgtt ccagtccctg acctttcctg cgaatcccgt ttccccgaca gctctcgtgg
     3361 tcagcatgat gtgcgcatgg ggattggtcc ggtccatgtg atggaacgcc acatcggcaa
     3421 tcatgccccg actgacaaag ttttcccgga caaaagcgag aacggtctcc cgtgcggcct
     3481 cgcgggaaat ctcacgggga atggcaagtt caatctccct tgccagctgg gagttccggc
     3541 gctgttcggc tttctcgacc gcattccaga gaacggaacg gtcaccacac caggacggtg
     3601 cattcgcagg ggtgagaatg accgcatcat caacgccgcg tttacgggtg taatcgtgga
     3661 ttaatccggt acggtcatcg ggtatgacag agccggaacg gtaggcagca gaagcaacag
     3721 cactgtagcc gttttttcgc gaaatgattt tcatgctgag atgataaatc gccatttctg
     3781 ccgtaatgcc tttttgccgt tttcttttag cgtcgcagac cgcacacaat cgaaacgaag
     3841 tggagattgt ataagtgcgc acttcgtgat gtgccgtgca ttatgccagt tcagttttgc
     3901 aactaccagt gacgcgctaa aatgcgattt ttcaccgcgc acggagtcac gaaatgagca
     3961 gaacactcga acagaagatt gccgaagctg aggccagatt acagcgcctg aaggcgaaga
     4021 gccggagcct ggacacggcg caaaaagtca ttgtgggggc tgccatgctg gcgagagtac
     4081 ggaggcctga agaagcgcaa ctgcgtgcgt tcctgctcca gtttctgcga aaggaagtca
     4141 caagacaggc ggatgtgaac cgactacaac cgctgattaa cgagctggag aagctaccca
     4201 gaccaccggc gaaaccgcag aaccactaag ccccggcgcg taagtgccca tgcaccaccg
     4261 ggcgcctttg cgcttcctcg ctcattcgct cgctacgctc ggtcatgaga ctgcggcgag
     4321 cgctaccacc tcaatcatgc ggaaagccac gccattacgg ggcagaacgg aggaaatcgc
     4381 aagaaagatc actgctcaaa aagacgccaa aagggcgctg ttatctgata aggcttatct
     4441 ggtctcattt tgcgagatct ggatcacatt ttaattttcg gtagtacgtc gctcgactga
     4501 tccctaatgc ctcccacggc ttcagcgaac gtgctgatgt aggaactgct gcgcgcttag
     4561 atttagttcc acccttgcga cctcgagcgg cctgtacagc ggaaaatccc tctggtgaga
     4621 atttcctgtg tgtatatttc gcaatgctct tgccaatagc ccgacattca gccggagaaa
     4681 gcggaacggg aagcgatgcg ttgtacattt cgacacgctg gatcacggca tcaagccatt
     4741 gtgagaatac aggccagccc tgacgaatcg ctctgtaggc ccatttacgg accttttcga
     4801 acagatggta gtttcgcccc agcccgtaat ttttatcgac gctacggcgc gctgaggcgc
     4861 tcaaatcgag ataatcagcc agttcgtcga gagtgtaggg ttcctcgcgc cattccactt
     4921 cctgccattc agggtggcac ggatttttgc agatcaggcc gctgtaattc acatccgcgc
     4981 ccagtttttc acacaacgca cgctcaatag cggcagcgta tctgagcgcc gaagccgatg
     5041 catccggcgc agttctcaca ggaagggcga gcgcgtagag cagatgcgcg tgcccgttac
     5101 ggggattttt tacggtgatg ttaggggccg gacaattccg gtcgctccag tcgattgatg
     5161 cccctgtccg gtccacgtca aagggaagcc agtacagtgc atgtggctgg ttctgctgga
     5221 tgaaacgggc aagaatggca atgtttttcg gcagaatgcg aacgccgtac gcgaaatcgt
     5281 tcgtacagta cggtttgtgc ggtaattttt ccctgaagcg ttgaagtacg gcgctcatgt
     5341 gatacgattt ctctcggtac acagtttaac taaccgcgct ttcccatcgg gaaacgcgga
     5401 acccggcgcc ctgtaatttc gtgatcaatc ttggcggtga gagcacgatt tacggggcgt
     5461 ttgtttttca gtcgcttaca ctacacccaa cggcaaatcc tgtcgatcct tcccgaaaaa
     5521 ccgatccatt taccataact cccgttgtac gtacccctta aaacaccctc aatcgctttc
     5581 tgacgcaaaa aaaacaaaaa gacaaggaaa caccagggta aacataaaaa atgcgtcaga
     5641 atcgttttta gcgcgttgca taactatgca cgaaagcgat gcacgatcgg tttcgtgcgt
     5701 tctgaacacc gaacggctca aaacgacaag ttttctcccc ctggatggaa agttctgcac
     5761 ggggatttta gatcccatct cccacatgga atttttgtga cacttcaaaa aaaagcgcgt
     5821 cagaatcagc ctgacacgct ttgtttttgt caaagaggat ttcgggttac tgcgttgcta
     5881 atccggtcac ttcagctgtt gatgacggag atacggtccc gccctgaaca tcccggatat
     5941 agtttgcctg acatgcagcc agaataatga ctgcaagaag caataaaata atccctgtta
     6001 tttttttcat aacgactcct tgttgtgaaa gaatgccagt cattcatcct taaacaactg
     6061 gcattcatat ttcagccctg tttaaatcct gacttaccgt tagcagctcg ccattcttta
     6121 atctctttaa taacagcttc agggctacca tcattattac cttctgggta ataaatcaga
     6181 tcagaaccac taggatgctc agtaacactt ataaaatgct cgaggttatc atcctgtttt
     6241 ttttcatcac cttcacaatt gatgatgtct tcaataattt ttttgaattc agtttcagtg
     6301 taatcactaa tgctgttttt cagttccatt tttactgacc tctgtgaata tcaatatggc
     6361 gtttcggcgt agtgatacgg aggttgtcca tatcataaac accaccatcc tgactgattg
     6421 gcttgtcgtg atgaagctca aatgagcgac gcccaccgac agtatcttta ttcctagccc
     6481 gtggagctaa tccttggctt aagcgttttt tattacctgg attaaattgc ttacttaact
     6541 caggatcttt cgacacttct tcccagaatt ttctccgaaa atcgtcgaag tttttaaatt
     6601 ctttatcacg caacttatca gcaatgcgat ctggaattgg cgctcctgaa tctttacctg
     6661 catcatccag ccatttatca ccaactggtt tacctttacc tgtcgccttc cctggcttat
     6721 tccgtttact ctccttatct aatttatcct tagc
//
