LOCUS       NZ_CP051742             6265 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-105 plasmid pSCU-105-4, complete
            sequence.
ACCESSION   NZ_CP051742
VERSION     NZ_CP051742.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609770
            Assembly: GCF_012934455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:54:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,101
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 64 of 195
            Pseudo Genes (incomplete)         :: 142 of 195
            Pseudo Genes (internal stop)      :: 39 of 195
            Pseudo Genes (multiple problems)  :: 44 of 195
            Pseudo Genes (short protein)      :: 4 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051742.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-105"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-105-4"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            276..584
                     /locus_tag="HHJ23_RS25795"
                     /old_locus_tag="HHJ23_25810"
     CDS             276..584
                     /locus_tag="HHJ23_RS25795"
                     /old_locus_tag="HHJ23_25810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001292104.1"
                     /translation="MSELEMSLTELITTIVQDPLFKVKAAGKALLNQNDGYHILMAIHE
                     HGEQAVQIEMAKQIAAREKMSFTEAARKASYYIEYAVMASNGDGYGKTTRNELNSKG"
                     /name="hypothetical protein"
     gene            1356..2354
                     /locus_tag="HHJ23_RS25805"
                     /old_locus_tag="HHJ23_25820"
     CDS             1356..2354
                     /locus_tag="HHJ23_RS25805"
                     /old_locus_tag="HHJ23_25820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234361.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_001082277.1"
                     /translation="MNRTETLKKIQIEVEEFVTYADNEQLIANHDCLTVLKKIPNNSIS
                     LILTDPPYHATQKKNIYGDTQFKKDDEYINWMEEISIEWRRILKPNGSLYCFCDSSMSA
                     RLEVMLSKRFNILSHIVWTKPNQPGFDGWKGKMNKESLRQWYGHSERIIFCEPAKDGNL
                     HRSSFGEFLKKRRKEAGLSGNKLTELTGAYGKVNHGGAVSNWETGRNIPSREQYKKIKE
                     AILNTGKVSFMPEYEDAIRPFIISKNVEYTDVWNFESVRPYKGKHPAEKPQDMLSHIII
                     ASSYEGDIVLDCFAGSGSTALSAKRNKRKCISIDIDTHWTDYIEKRLNELF"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(2368..3354)
                     /locus_tag="HHJ23_RS25810"
                     /old_locus_tag="HHJ23_25825"
     CDS             complement(2368..3354)
                     /locus_tag="HHJ23_RS25810"
                     /old_locus_tag="HHJ23_25825"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BstXI family restriction endonuclease"
                     /protein_id="WP_000533217.1"
                     /translation="MGRNIKLPILLSRKIYKTGQTRGADDDVIYQNRVSRNSTVLIPFE
                     LIISAHNRYVLRKNYDNGYIVLIPPDYLFGEMRDETISIMNDCGLVLGVNCLVFYSQRR
                     QYEKYLPDKFGLRYTQPMTRTGDLGGDYVCRISGTTSENMRKISFGYNLTTSKGAGIRL
                     FEYADKEVIEKARIQLEAIYWLCNDSLDAAIEYGMSSEDARERKRLAIDEAKRQGLLNE
                     VELIKNRIIDVDKDTVCPLCLAKISAKGFFSKVEQQEFRRVHDLTVTEVNLFHINELRY
                     GEFNHKPYNLGWGHHHCNVVVKDSGIDNTLEWMHEILVRNERLNKAP"
                     /name="BstXI family restriction endonuclease"
     gene            complement(3338..3586)
                     /locus_tag="HHJ23_RS25815"
                     /old_locus_tag="HHJ23_25830"
     CDS             complement(3338..3586)
                     /locus_tag="HHJ23_RS25815"
                     /old_locus_tag="HHJ23_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107566.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_071529142.1"
                     /translation="MENLLLSKISFVIKKIRLEKGMTQEDLAYKSNLDRTYISGIERNS
                     RNLTIKTLGLIIKGLGVSNIVFFELLVKEISTHGQEY"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4247..4480)
                     /locus_tag="HHJ23_RS25820"
                     /old_locus_tag="HHJ23_25835"
     CDS             complement(4247..4480)
                     /locus_tag="HHJ23_RS25820"
                     /old_locus_tag="HHJ23_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137440.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4487..5017)
                     /locus_tag="HHJ23_RS25825"
                     /old_locus_tag="HHJ23_25840"
     CDS             complement(4487..5017)
                     /locus_tag="HHJ23_RS25825"
                     /old_locus_tag="HHJ23_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001483786.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINENMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5055..5486)
                     /locus_tag="HHJ23_RS26335"
                     /pseudo=""
     CDS             complement(<5055..5486)
                     /locus_tag="HHJ23_RS26335"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5695..6018)
                     /locus_tag="HHJ23_RS25835"
                     /old_locus_tag="HHJ23_25850"
     CDS             complement(5695..6018)
                     /locus_tag="HHJ23_RS25835"
                     /old_locus_tag="HHJ23_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001483787.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQRVQ
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6083..>6265
                     /locus_tag="HHJ23_RS25840"
                     /old_locus_tag="HHJ23_25855"
     CDS             6083..>6265
                     /locus_tag="HHJ23_RS25840"
                     /old_locus_tag="HHJ23_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_076140113.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          664..1027
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.986"
                     /coverage="89.0"
                     /evalue="1.26e-172"
     Typing          complement(4144..5705)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.545"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aacctctgac acatacagct cccggagacg gtcacagctt gtctgtgagc ggatgccggg
       61 agcagacaag cccgtcaggg cgcgtcaggg ggtttcgggg cgaagccctg aaccagtcac
      121 gtagcgatag cggagtgtat actggcttag tatgtcagaa tcggaggcag tggtgcgaaa
      181 atttagagcg gatttaaggc gtttgggaag ggcataatga taaagcttga attaagggtg
      241 ttagccaata tattgaagga ttaccgtcca catatatgag tgaactggaa atgagtctga
      301 cagaactaat tacaacgata gtacaagacc cactgtttaa agttaaagca gcagggaaag
      361 cgttattaaa tcagaatgat ggctatcaca tactaatggc gatccatgag catggtgagc
      421 aagcggtaca gatagaaatg gcaaagcaga tcgcagctag ggaaaagatg tcatttacgg
      481 aagcagcgag gaaagcatcc tattacatag aatatgctgt gatggcttct aatggtgatg
      541 gatacggaaa gacaactcga aacgagttaa attcgaaagg ttagttccaa aaaccatata
      601 taagatcaac cgagacaggc tgtgatctta tgtaggctaa ctagtgatag gagcctttgc
      661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaaccgacgc tcaagtcaga
      721 ggtggcgaaa cccgacagga ctataaagat cccaggcgtt tccccccgga agctccctcg
      781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      841 gaagcgtggc gctttctcat agctcacgct gttggtatct cagttcggtg taggtcgttc
      901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gtcttatccg
      961 gtaactatcg tcttgagtcc aacccggtaa aacacgactt aacgccactg gcagcagcca
     1021 cttgtaaccg gattagcaga gcgagattta taagttggta acagagttct tgaagtatca
     1081 gccatattct ggctacacta gaaggacaag ttttaggtat ctgcgctctg ctgaagccag
     1141 ttacctcggt tagataatct gccaggatta actaaccttc gaaaaaacca cctgccaggg
     1201 tggttttttc gtttacatag caagagacta catctcactg cttaggatct caagaggatc
     1261 cgctctacag aacttatcta tcaaataatt aaaagattga aaaaaaaacc ttggatgtta
     1321 gaatgttgac tatagtcaac aaaagaaaaa tggttatgaa cagaactgaa actttaaaaa
     1381 aaattcagat tgaagtcgaa gagttcgtca cttatgcaga taatgagcaa ttaatagcca
     1441 atcatgactg tttaactgta ttaaaaaaga tcccgaacaa ttcaatttct ttgatactta
     1501 ccgatccacc atatcatgca acacagaaaa agaatattta tggtgatact caattcaaaa
     1561 aagacgatga atatataaat tggatggaag aaatctccat tgaatggcga agaatattaa
     1621 aaccaaatgg ttctctttat tgcttctgtg attcatcaat gtctgcacga ttagaagtta
     1681 tgctatctaa acgatttaat atattaagtc atattgtatg gactaaacca aaccaacctg
     1741 gctttgatgg ttggaaaggg aagatgaata aagaatcatt acgtcagtgg tatggacatt
     1801 cagaaaggat tatcttttgc gaacctgcaa aagacggaaa tttacatcgt tcttcttttg
     1861 gtgaattctt aaaaaaaaga agaaaagaag caggtcttag cggaaacaag ctaactgaat
     1921 taacaggcgc ttacggaaaa gttaatcatg gcggcgctgt atctaactgg gaaacgggaa
     1981 gaaatatacc tagtcgcgaa caatataaaa aaataaaaga agccatatta aatacaggga
     2041 aagtgagctt catgcctgaa tatgaagatg caatacggcc ttttattatt tcaaaaaatg
     2101 tagaatacac tgatgtttgg aattttgagt ctgttagacc atataaagga aagcatccag
     2161 cagaaaaacc acaagatatg ttaagtcata ttataattgc tagctcctat gaaggcgaca
     2221 tagttttaga ttgttttgct ggttctggta gcaccgcatt atcagcgaaa agaaacaaac
     2281 gaaaatgcat atctattgat atagatacgc attggacaga ttatatagaa aaaagattaa
     2341 atgaactgtt ttaaacgcag agcgccttta tggcgcttta tttaaccttt catttctaac
     2401 caaaatttca tgcatccact ctaatgtatt atcaatgcca gaatctttta ccaccacatt
     2461 acaatgatga tgcccccacc ctaaattata tggtttatga ttaaactcac cataccttaa
     2521 ctcattgata tgaaaaagat ttacttctgt tacagtaaga tcatgtactc ttctaaactc
     2581 ttgctgttct acttttgaaa aaaaaccttt agccgatatc tttgctaaac ataatgggca
     2641 aacagtatct ttatcaacat caattattct attttttatc aattcaactt catttaatag
     2701 cccttgtcgt tttgcctcat caatggctaa tctttttctt tccctagcat cttcagatga
     2761 cattccatat tctatagcgg catctaatga atcattacac aaccaatata ttgcctcaag
     2821 ttgaatacgc gccttttcta ttacttcttt atcggcatac tcaaacagtc taattcctgc
     2881 ccccttagat gttgtaagat tatacccaaa tgatattttt ctcatatttt cagaagttgt
     2941 tccagaaatt ctgcaaacat agtcaccacc aagatctcca gtccgagtca ttggctgggt
     3001 atacctaagc ccaaatttat ctgggagata tttttcatac tgacgcctct ggctataaaa
     3061 aaccaagcag ttaacaccta aaactaatcc gcaatcattc ataatagaaa tggtttcatc
     3121 acgcatctct ccaaacaaat aatcaggcgg aattagaaca atataaccat tatcatagtt
     3181 ttttcttagc acatatctat tatgcgcaga tattatcagc tcaaatggta ttaaaacggt
     3241 gctatttctt gaaactctat tctggtaaat tacatcatca tcagctccgc gagtctggcc
     3301 tgttttgtat atttttcgcg ataataaaat aggaagctta atattcctgc ccatgtgttg
     3361 aaatctcctt tacaagtagt tcaaaaaaaa caatatttga tacccccaag ccttttataa
     3421 ttaacccaag agtttttatt gttaaatttc ttgaattccg ttcaatcccg gaaatataag
     3481 ttctatctaa gtttgattta tacgccagat cttcttgagt cattcctttt tctagtctta
     3541 ttttctttat tacaaaagat atttttgata aaagaagatt ttccataact aatatttttt
     3601 gttcgttttt ggtggtatca tcatgcctcc atgtcgacta tagttacacg gactataagt
     3661 cacatcatta attcattaat acggcaaatt ccatttacca taacgtcgca ttgtacgcac
     3721 cactaaaacg cgttcggtgc gatcacggca gcagacaggt aaaaatggca acaaaccacc
     3781 cgaaaaactg ccgcgatcgc accaggtaaa ttttaaccac atgcatagct atgcagccat
     3841 gtgaatcacg caggaatttc cagccagaga ctgctgaaac ggattttttt catactttcg
     3901 gaaaggcaga agacggaacc ggagcggaaa aacagaaatt cagaggaatg agcgcgatct
     3961 ggcaataacg gcaaaacaca gcaacaaaag atacaccaga atcgcgcccg tatgcgtttt
     4021 aacgcgttcc tgtgttttca gcaaggtctg actgaagcgc gttcccggtg cccccgaacc
     4081 aaccattatc ccaaaaaatg caccggaaac gcgctgacaa cattttagcc tgtctttggt
     4141 taccatccca gcgaagggcc atccagtgtg cgttcctgta tttccggctg gcgctcacgc
     4201 tccagggata acgcatgttc gcgctcctgt atcagccgtt cgtcccttat ctcctgttct
     4261 cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc tgctgactca
     4321 actgcatgac ccgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa aacattttct
     4381 gcaattccac gaaggcgctc tcccattcgt tcagccgctg catatagtcc tgttgcagtt
     4441 gctctaaggc gttcagcaaa tgtctttcca gctccgtcat gctctgtcac tcctgcacca
     4501 gtctcactgc gttcagtttg ccgttgtcct tcgtccagtt cgtttcggct ttcatcccct
     4561 tcggcaacac cagaaaacgt ccgttgctgt cctcgtgata acgcacgccc catgtcttcg
     4621 cgttcagctt ctccagactc tctttctgct cgcggagaat attcatattc tcgtttatct
     4681 tcatgccctg gtaccacagc agactcccgg tcgtggcaaa catcagtact gagacgataa
     4741 tcagccacag ccagcgtttc acgctcattt tcagcacggt ttgccagtgt gtgtccatcg
     4801 tgctgacggt cgttttctga tacctctcca gaagttcttt cagggctgct gtatggtcgt
     4861 tgatagcgtt cctgattaaa gattcgtttg aggtcagttc cctgtttaca gattcttcga
     4921 gcttcctgaa ctcgtcgttc agcattgtct ctgtagattc ggctctctct ttcagctttt
     4981 tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa atgctccttt cagcctgata
     5041 ttcttcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct gacaacttca
     5101 agccccgcct ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc cccgacatga
     5161 taaagagcgt ctatcccccg tgtaacgctc tcagcaagcg cctgtttcgt ttccggcagg
     5221 ttatcaggga gagtcagtat ccggcggttc tccggggcgt tggggtcatg cagcccgtaa
     5281 tggtgattca ccagcgtctg ccaggcatca attctcggcc tgtctgcccg gtcgtagtac
     5341 ggctggaggc gttttccgct ctgtagctcc atgttcggaa tgacaaaatt aagctcaagc
     5401 cgtcccttgt cctggtgctc cacccacagg atactgtact gattcttttc gagaccgggc
     5461 atcagtacgc gctcaaagct cgccatcact ttttcacgtc ctcccggcgg cagctccttc
     5521 tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt
     5581 tcccggactt cttccggatt accctgaagc accgttgccc cttcccggtt tcgctccctt
     5641 cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg aaacttaact
     5701 atcatcctgc actctctgtt ccctgaccac ctgccgtaag ctgcgcaact ctctctcgat
     5761 ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc cgctgttcac
     5821 cttcctcgca gtctgattca ggttattccc gatggcggcc agctgacgca gtaacggcgg
     5881 tgccagtgtc ggcagcctcc cggagcgggc aaccggctca cccagacaga cccgccgcat
     5941 ccataccgcc agctgtttac cctcacagcg ttcaagtaac cgggcatgtt catcatcagt
     6001 aacccgtatt gtgagcatcc tctcgcgttt catcggtatc attaccccca tgaacagaaa
     6061 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     6121 ctttataaga agccagacat taacgctgct ggagaagctc aacgaactgg acgcagatga
     6181 acaggccgat atttgtgaat cacttcacga ccacgccgac gagctttacc gcagctgcct
     6241 cgcacgcttc ggggatgacg gtgaa
//
