LOCUS       NZ_CP051741             7939 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-105 plasmid pSCU-105-3, complete
            sequence.
ACCESSION   NZ_CP051741
VERSION     NZ_CP051741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609770
            Assembly: GCF_012934455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:54:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,101
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 64 of 195
            Pseudo Genes (incomplete)         :: 142 of 195
            Pseudo Genes (internal stop)      :: 39 of 195
            Pseudo Genes (multiple problems)  :: 44 of 195
            Pseudo Genes (short protein)      :: 4 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051741.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-105"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-105-3"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            complement(<1..76)
                     /locus_tag="HHJ23_RS25735"
                     /old_locus_tag="HHJ23_25750"
                     /pseudo=""
     CDS             complement(<1..76)
                     /locus_tag="HHJ23_RS25735"
                     /old_locus_tag="HHJ23_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312460.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            117..308
                     /locus_tag="HHJ23_RS25740"
                     /old_locus_tag="HHJ23_25755"
     CDS             117..308
                     /locus_tag="HHJ23_RS25740"
                     /old_locus_tag="HHJ23_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1673..2371
                     /locus_tag="HHJ23_RS25750"
                     /old_locus_tag="HHJ23_25765"
     CDS             1673..2371
                     /locus_tag="HHJ23_RS25750"
                     /old_locus_tag="HHJ23_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            2410..4002
                     /locus_tag="HHJ23_RS25755"
                     /old_locus_tag="HHJ23_25770"
     CDS             2410..4002
                     /locus_tag="HHJ23_RS25755"
                     /old_locus_tag="HHJ23_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_169063311.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNAIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            4005..4898
                     /locus_tag="HHJ23_RS25930"
                     /old_locus_tag="HHJ23_25775"
     CDS             4005..4898
                     /locus_tag="HHJ23_RS25930"
                     /old_locus_tag="HHJ23_25775"
                     /inference="COORDINATES: protein motif:HMM:NF019619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(4923..5285)
                     /locus_tag="HHJ23_RS25765"
                     /old_locus_tag="HHJ23_25780"
     CDS             complement(4923..5285)
                     /locus_tag="HHJ23_RS25765"
                     /old_locus_tag="HHJ23_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(5282..5521)
                     /locus_tag="HHJ23_RS25770"
                     /old_locus_tag="HHJ23_25785"
     CDS             complement(5282..5521)
                     /locus_tag="HHJ23_RS25770"
                     /old_locus_tag="HHJ23_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            complement(6232..6465)
                     /locus_tag="HHJ23_RS25775"
                     /old_locus_tag="HHJ23_25790"
     CDS             complement(6232..6465)
                     /locus_tag="HHJ23_RS25775"
                     /old_locus_tag="HHJ23_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6472..6990)
                     /locus_tag="HHJ23_RS25780"
                     /old_locus_tag="HHJ23_25795"
     CDS             complement(6472..6990)
                     /locus_tag="HHJ23_RS25780"
                     /old_locus_tag="HHJ23_25795"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<7028..7459)
                     /locus_tag="HHJ23_RS26330"
                     /pseudo=""
     CDS             complement(<7028..7459)
                     /locus_tag="HHJ23_RS26330"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7668..>7939)
                     /locus_tag="HHJ23_RS25790"
                     /old_locus_tag="HHJ23_25805"
     CDS             complement(7668..>7939)
                     /locus_tag="HHJ23_RS25790"
                     /old_locus_tag="HHJ23_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_250852611.1"
                     /translation="EGKQLAVWMRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQT
                     ARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGARDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(385..464)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          497..1039
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(6129..7678)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7675..7937)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.318"
                     /coverage="97.0"
                     /evalue="5.37e-47"
ORIGIN
        1 agcgttccag taaccgggca tgttcatcat cagtaacccg tatggtgagc atcctctcgc
       61 gtttcatcgg tatcattacc ccataaaaca gaaatccccc ttacacggag gcatcagtga
      121 ctaaacagga aaaaaccgcc cttaacatgg cccgttttat cagaagccag acattaacgc
      181 tgctggagaa actcaacgaa ctggacgcag atgaacaggc cgatatttgt gaatcacttc
      241 acgaccacgc cgacgagctt taccgcagct gcctcgcacg cttcggggat gacggtgaaa
      301 acctctgaca catacagctc ccggagacgg taacagcttg tctgtgagcg gatgccggga
      361 gcagacaagc ccctcagggc gcgtcagggg gtttcggggc gaagccctga accagtcacg
      421 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagtacgaat tgtatggaaa
      481 gcgcaccatg tccgatgtga aatgccgcac agatgcgtaa ggagaaaatg cacgttccgg
      541 cgctcttccg cttcctcgct cactgactcg ctacactcgg tcgttcggct gcggcgagcg
      601 gtgtctgctc actcaaaagc ggagatgcgg ttatccacag aatcagggga taacgccaaa
      661 agaaacatgt gagcaaaaaa cgagaaccag gaaaaggctg cgccgttggc gtttttccat
      721 aggctccgcc cccctgacga gcatcataaa aatcgacgct caagtcagag gtggcgaaac
      781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      901 ctttctcata gctcacgctg taggtatctc ggctcggtgt aggtcgttcg ctccaagctg
      961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1021 cttgagtcca acccggtaag acacgacaaa gcgccactgg cagcagccac tggtaactgg
     1081 acaaggtgga ttgagatatt aagagttctt gaagtggtgg cctaactgcg gctacactag
     1141 aaggacagta tttggtatct gtgctccacc aagccagtta cccggttaag cagtccccaa
     1201 ctgacttaac cttcgactaa accgcctccc caggcggttt tttcgtttac tggcagcaga
     1261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat ctttttgaga tccgcccact
     1321 cctggaacct acaacacatg tgtaaaacgt caatggaggg ggctattatc ggactgcaac
     1381 acatttcgta cagcctttac actcggtgaa ttagcggccc tagatgcatc cactcattca
     1441 accaactcaa ttctcttctc ttagagtggg aaaattttgt tttccttgca gcccctaccc
     1501 gtaaaaactg gctgcaaaaa ccttgtatta ctaatggatc ctataaccat taaaaaactt
     1561 gattactatc tcatttatag aattaggcat attgacacaa gcacaaattt aacaatacca
     1621 taacaaatga taactattct catctacatt caaatatata attgggggtg ttatgctaca
     1681 acataagatg aacagcagtt cttatgcaaa agttcataat gttagctcat tagaagatat
     1741 catgagttat cacaatgatg atgttcttct aaaatttcgt aaagaatgga atgtaacacc
     1801 agaagaagct gatgatatct ttaatgaaac aaagaaattc atctggctag cctcaacatg
     1861 cctaacggaa tgctacaata taaaagttca cgagcaatta caaattatag atgaaatgtg
     1921 gcatacgttt attcaattta cagatgctta taccagcttt tgtgaaaaat atcttggtgc
     1981 ttacctccac cattatccaa atacaaatga catgctaaaa aatgagataa ggcatgttaa
     2041 tgagcatggt ataacattcc aagaatatcg ttttaacgaa tataaaaatc aaattgaaaa
     2101 aatcgctttt tacctaggtc atgaaactgt cgcaaagtgg tatggtgatt atgctgtaag
     2161 atacagcata aagaacatta atactataag aattccaaaa gaatccatat ccagtgattc
     2221 ttacatcgaa aaagtaaaga gtatcactca ccttccagcc gcagaatttg taaaaataat
     2281 aatgcgaaaa gatgtttgga atgataatgg ttctgtttgt ggttgcagcg gtaaaggatg
     2341 tggcgctggg tgctcatgta attctagata acataaagcc cgtaatatac gggctttaag
     2401 gattataaat tgaattttat tgaaaagtac atcatcacat ttaataaatg taatttgttt
     2461 tttattatat tattatcaac tataagtagt tttcttttcg ttctattcgg atatttaata
     2521 aaaacagtta ttgacaataa agattcaata ggagaaataa actcattatt aatatttata
     2581 gcatgtttct tatcaattag attccttatg cctgctggat atagcatatc ggaatacttg
     2641 actcaaaaaa caaatataga attatctgta aagcttagag agcaagttat tgataatata
     2701 caaaattctc accaagagca ttttttaaag aaaaataaag gagaactaaa taaggttata
     2761 gaaagcatgc tttcttctgc atcatcatta ttttatacaa tatgttctga tgtagtacca
     2821 ttattaatac aaatgattgg aataatcata acaatttgta taagtgttaa tacattaata
     2881 gccattgagt ttataataat aatggcaatc tatataatat ttgtcataaa aatgacacaa
     2941 cgtagatttc caatgatgaa atcagttgca cttagctcca aattcgcatc tggaagaatg
     3001 tttgatatga tgcacatgta tcctatggat aaagctttcc ataccacgga taaaagtcgt
     3061 aaacgagtaa ttcaagctgt aaatacacat tcagaaaaac aaagaaaagt aaataatgaa
     3121 tttttcctgt ttggtatcag ctccgctttt ctttcagtca tttttagtag tctgattatc
     3181 ctatcagcat actggatgtt tcttcatggt agagcaagcc gtggtagtat tattatgctt
     3241 gccacttttt tattccaagt ttttctccca ttaaatcgca ttggttatct atttagacaa
     3301 ataaaaatgg caagaacaga aattgattta tactgtgccg aaataaacga cataaaaaaa
     3361 acaaattaca cagacaagca atatcttcat gtaaaagaca atatttgcaa tattgaaata
     3421 tacaacaaat cattcaacca taaattaata ttaacaaaag gcgttgttac ctttataacc
     3481 ggtgagaatg gttcgggaaa aacaactatt gcaaaaattc tctctggcaa tataacaaca
     3541 gaagaaaaca caataaaaat caatgggata caacaagaaa aagcaaattc tccttttgtt
     3601 aatgtcttat acgttcctca agatctagat ctcatgcctg gaactataga ggaaaatata
     3661 ttacattatt ccggcattaa taatttaaat gcaattaaaa agctgctaca cagatttaag
     3721 ttcgacaaac cactagatta tgagatcaaa ggttacggac ataatttatc tggtggacaa
     3781 aaacaaaaat taggagtatc tcttacatct ggaaaaaacg tagacttgat tattttcgat
     3841 gaaccgacaa aagggtttga ttcacttggt ataaaaataa tcagtgatta tataaaagag
     3901 gaaaacaagg aaaagcatat cattgttata tctcatgatg aacaacttat aaataatata
     3961 cctaaagcac agattataga tatcacaaaa catgaggttt aaaaatgaat aatcttataa
     4021 aaaaggaaat catagaaaaa tttaagaaat ataatttcca gaaatatccg tttgtattca
     4081 cggatgttaa ttataaaaac ttaatcaact ggaatgatct taataaattg cttgaaaaag
     4141 atatattgca ctatcctaga gttagaatgg caaatgacaa ctttcccgaa attagggggt
     4201 ataaaggatt tataagatac acatatagcc aaacaggcga tagaacacca catataaatc
     4261 gccatcagct atataaatgc ttacgtgatg gcgcaaccct tatagtagat cgatgccagt
     4321 cattctttga atctgtagat gaaagcagac tatggttatc taaagagtta gaatgtacat
     4381 gtagtgctaa tctatatgct gcatttacag caacaccaag ctttgggctt cattttgaca
     4441 atcatgatgt aatagctgtt caaattgagg gaataaaaaa atggaaagtt tacaacccta
     4501 cctattcata ccctctcgaa gatgaaagaa gcttcgatta tctaccacct aatacctccc
     4561 cagattatga gtttgatata accccaggtc aggccatata tcttcctgct ggatactggc
     4621 acaatgttac gactcagagc aaacactctc ttcatatatc ttttacagtt ataagacctc
     4681 gtcgattaga actatttaaa acattatttg atgaattaaa aaacaatcca tatatgcggg
     4741 aacctataga gcatggtgat tcattatctg ataaagaaaa aataaaaact ataataacta
     4801 atgctataaa tagttttgat attcagtttg cagaaaacat gcttaaagct caaagcagaa
     4861 cttatcgcta taaaaaaata aaacttgaac atatataacc attaatctaa gggggcttcc
     4921 ccttagattt tttcatacga tataaactgc attttccatc caccattaac ctttatccaa
     4981 ttctcaataa aagccattaa tatatttcca tcattttgct ttatttcagc tttccctgaa
     5041 acacaagcaa tgcctttaaa ctcacgcatt acgacatcat attcatatct ttctatgcta
     5101 ggtaaaatcc tcgctggacg catatcaatc tttcgtaact gatgtttttt atagataact
     5161 ttttgaccat ttgttgaaat aaaccaatca gcttccaagt aatctagcat ttcagtatta
     5221 cctgaataaa atgcattata ccattctttt cttattagat ttaagtctga tttaaaatta
     5281 gtcacactac cttcctcata tctaattcct cctctaatct atccccaaca ttccttactg
     5341 attttatagc atcatcaata gccacattat atatgactgg gattactctt ttaagaatct
     5401 catcaagtaa ttcctctgat tcaaactgtc caaggcatat atcatgttca tctctgattt
     5461 tttgtgaaat aaattcagct agtcctttat atatgttttt ttcaatatca gaaattttca
     5521 ttctttatga agcctcacaa ataaattaat aatataacat ttttcctatc ataggaaaat
     5581 tacaactcaa ccatactgca acctggaatt tcccaagcaa gcatatttaa ataagtgcag
     5641 gataccacct caaatcattc tctaaggaac aagataattt accataatcc cttattgtac
     5701 gcaccgctga aacgcgttcg gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac
     5761 cacccgaaaa actgccgcga tcgcacccgg taaattttaa ccacatgcat agctatgcag
     5821 ccatgtgaat cacgcaggaa ttgccagcca gagacagctg aaacggattt ttttcatgct
     5881 ttcggaagcg aagaagacgg ggaccggagc gggaaaacag aagttcaggg gaatgagcgc
     5941 gatctggcaa tagaggcaaa acacagcaac aaaagacaca ccagaatcgc gcccgtatgc
     6001 gttttaacgc gttcctgtgt tttcagcaag gtctgactga agcgcgttcc cggtgccccc
     6061 gaaccaacca ttatcccaaa aaatgcaccg gaaacgcgct gacaacattt tagcctgtct
     6121 ctgattacca tcccagcgaa gggccatcct gtgtccgttc ctgtatttcc agctggcgct
     6181 cacgctccag ggataactca tgttcgcgtt cctgtatcag ccgttcatcc cttatctcct
     6241 gttctcgctg tataactggc tcaagcgttc tgtctgctcg ctcaagtgct gcacctgctg
     6301 actcaactgc atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat
     6361 tttctgcaat tccacgaagg cgctctccca ttcgttcagc cgctgcatat agtcctgttg
     6421 cagttgctct aaggcgttca gcaaatgtct ttccagctcc gtcactctgt gtcactccgt
     6481 cagttgtacc cagtccttcc cctgataggg aatcaccgtt gccgatttcc cctgagggat
     6541 aaccagaaat ttccttctcc cgtcctgcac aaactccacg ccccatgtct tcgcgttcag
     6601 tttctgcaat gtttcttcct gtatcctgat ttcttccagg ttcgcctgta tcatcccgcc
     6661 aagataccag agcgtcccgc cactcacggt aaacaggaaa aggaccatcc ccagtaacat
     6721 catgcccgta ttccctgcca gctttaacac gtccttcctg tgctgcatca tcgcctcttt
     6781 caccccttcc cggtgttttt tcagtgattc ctctgtcgaa gctgtgaaca gggctatagc
     6841 gtctttgatt ttcgtctcgt ttgatgtcac agccttgctt acagattttt cgagcttgct
     6901 gaactcgtcg ttcagcattg tctctgtaga ttcggctctc tctttcagct ttttctcgaa
     6961 ctccgcgccc gtctgtaaaa gattgctcat aaaatgctcc tttcagcctg atattcttcc
     7021 caccgttcgg gtctgcaatg ctgatactgc ttcgcatcac cctgaccact tcaagccctg
     7081 cctctgtgag cgcctgaatc acatcctgac gaccttttat ctccccgaca tgataaagag
     7141 cgtctatccc gcgtgtgacg ctctctgcaa gcgcctgttt cgtttccggc aggttatcag
     7201 ggagagtcag tatccggcgg ttctccgggg cgttggggtc atgcagcccg taatggtgat
     7261 tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgga
     7321 ggcgttttcc ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct
     7381 tgtcctggtg ctccacccac aggatgctgt actgattctt ttcgagaccg ggcatcagta
     7441 cacgctcaaa gctcgccatc accttttcac gtcctcccgg cggcagctcc ttctccgcga
     7501 acgacagaac acctgacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga
     7561 cttcttccgg attaccctga agcaccgttg ccccttcccg gtttcgctcc cttcccagca
     7621 ggtaatcaac cggaccactg ccaccgcctt ttcccctggc atgaaattta acaatcatcc
     7681 cgcgcgccct gttccctgac ggccagacgc agccgacgca gctcctcccc gatggccatc
     7741 agcgcagcca acacctgaac ccgatcaccg gaagaccact gcccgctgtt caccttcctc
     7801 gctgtctgat tcaggttatt cccgatggcg gccagctgac gcagtaacgg cggtgccagt
     7861 gtcggcagct tcccggagcg tgcaaccggc tcacccaggc agacccgccg catccatacc
     7921 gccagctgtt taccctcac
//
