LOCUS       NZ_CP051740             9703 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-105 plasmid pSCU-105-2, complete
            sequence.
ACCESSION   NZ_CP051740
VERSION     NZ_CP051740.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609770
            Assembly: GCF_012934455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 57x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:54:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,221
            CDSs (total)                      :: 5,101
            Genes (coding)                    :: 4,906
            CDSs (with protein)               :: 4,906
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 64 of 195
            Pseudo Genes (incomplete)         :: 142 of 195
            Pseudo Genes (internal stop)      :: 39 of 195
            Pseudo Genes (multiple problems)  :: 44 of 195
            Pseudo Genes (short protein)      :: 4 of 195
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051740.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-105"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-105-2"
                     /geo_loc_name="USA: California"
                     /collection_date="2015"
     gene            complement(484..1068)
                     /gene="mphR(A)"
                     /locus_tag="HHJ23_RS25680"
                     /old_locus_tag="HHJ23_25695"
     CDS             complement(484..1068)
                     /gene="mphR(A)"
                     /locus_tag="HHJ23_RS25680"
                     /old_locus_tag="HHJ23_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="WP_001137892.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
                     /name="mphR(A)"
     gene            complement(1068..2306)
                     /gene="mrx(A)"
                     /locus_tag="HHJ23_RS25685"
                     /old_locus_tag="HHJ23_25700"
                     /gene_synonym="mrx"
     CDS             complement(1068..2306)
                     /gene="mrx(A)"
                     /locus_tag="HHJ23_RS25685"
                     /old_locus_tag="HHJ23_25700"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="WP_000004159.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGLI
                     ACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAAIG
                     LLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLF
                     GIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISA
                     QNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLG
                     RVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAA
                     ATASAPTHTEGEH"
                     /name="mrx(A)"
     gene            complement(2303..3208)
                     /locus_tag="HHJ23_RS25690"
                     /old_locus_tag="HHJ23_25705"
     CDS             complement(2303..3208)
                     /locus_tag="HHJ23_RS25690"
                     /old_locus_tag="HHJ23_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000219391.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD
                     GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMV
                     IQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDV
                     DRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE
                     ARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA
                     LDSGNEEYLAAAKAQLAAAE"
                     /name="Mph(A) family macrolide 2'-phosphotransferase"
     gene            complement(3330..4034)
                     /locus_tag="HHJ23_RS25695"
                     /old_locus_tag="HHJ23_25710"
     CDS             complement(3330..4034)
                     /locus_tag="HHJ23_RS25695"
                     /old_locus_tag="HHJ23_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            4093..4797
                     /locus_tag="HHJ23_RS25700"
                     /old_locus_tag="HHJ23_25715"
     CDS             4093..4797
                     /locus_tag="HHJ23_RS25700"
                     /old_locus_tag="HHJ23_25715"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="WP_250852609.1"
                     /translation="MPNKVPQGGCCSSYIANLLFFNSEYNLYNRLKSMGLSYSRLLDDI
                     TISSDKDLSSEEKTKVIKLVHGMVNQYRLSINESKTTIEHSKDSSSKLSVTGLWVKHGV
                     PKLTKENRRYIRYLVYICKKQGAYERHTKEYHDLWNRCSGKVAQMSRLGHVQAVELRAI
                     LSEIMPVYDDYKISKLKLMAKHYLNKFTPPLTDDQIRKIDRMLYDFDIVGRTNKNLAKL
                     YRRKLVALLPDR"
                     /name="reverse transcriptase domain-containing protein"
     gene            4802..5005
                     /locus_tag="HHJ23_RS25705"
                     /old_locus_tag="HHJ23_25720"
     CDS             4802..5005
                     /locus_tag="HHJ23_RS25705"
                     /old_locus_tag="HHJ23_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="WP_000427298.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICKG
                     ILVRFKLEKTDKGFKAVAIQKV"
                     /name="retron Se72 family effector protein"
     gene            complement(5843..6037)
                     /locus_tag="HHJ23_RS25710"
                     /old_locus_tag="HHJ23_25725"
     CDS             complement(5843..6037)
                     /locus_tag="HHJ23_RS25710"
                     /old_locus_tag="HHJ23_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001485273.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6321..6644
                     /gene="mobA"
                     /locus_tag="HHJ23_RS25715"
                     /old_locus_tag="HHJ23_25730"
     CDS             6321..6644
                     /gene="mobA"
                     /locus_tag="HHJ23_RS25715"
                     /old_locus_tag="HHJ23_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(7028..7888)
                     /locus_tag="HHJ23_RS25720"
                     /old_locus_tag="HHJ23_25735"
     CDS             complement(7028..7888)
                     /locus_tag="HHJ23_RS25720"
                     /old_locus_tag="HHJ23_25735"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(8071..8628)
                     /locus_tag="HHJ23_RS25725"
                     /old_locus_tag="HHJ23_25740"
     CDS             complement(8071..8628)
                     /locus_tag="HHJ23_RS25725"
                     /old_locus_tag="HHJ23_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            8930..9694
                     /locus_tag="HHJ23_RS25730"
                     /old_locus_tag="HHJ23_25745"
     CDS             8930..9694
                     /locus_tag="HHJ23_RS25730"
                     /old_locus_tag="HHJ23_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     ARG             complement(2307..3208)
                     /name="mph(A)"
                     /gene="mph(A)"
                     /gene_name="Mph(A) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7032..7888)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2304..3208)
                     /name="mphA"
                     /gene="mphA"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1069..2306)
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          6073..6312
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.755"
                     /coverage="100.0"
                     /evalue="7.66e-118"
     Typing          complement(5287..5808)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.476"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          6322..6641
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.15e-62"
ORIGIN
        1 agcgacagcc tacctctgac tgccgccaat ctttgcaaca gagcccgccg tgctagtctg
       61 ctcggtgatg gtggagtgaa gccaacccgc aatcgggtta tgaatctgca tcgcgattcg
      121 caatcagctg tctcttgagc atgtcgaact cctgcgatgt tatctcgccg tgctcgtgct
      181 gctacaccaa cttgtccaac tcatccgcca caccgctccc caccacctgc gatggccagg
      241 ttgtttgctg ggcgcattgg cggagtatcg tctcgacccg ggacacccac accggcacaa
      301 ccttacggga gtgattcact gtcaaagaat cggcccggtg ctctgacgca agtatcggga
      361 tggtcaccat ttgtaagccg tagacctgag tggtgatcaa gacttcgata ccaccgaccg
      421 taccggtact aatcgacgac ggtcgtgttc gtcgcctgcc gcagggactc tgcacacctc
      481 cgtttacgca tgtgcctgga ggagttggaa atcgtcgtgt tcgggaaaca ttaaacacag
      541 gatggcagcg atctgagcca gcacatgatc agctagctca ccatccggat cgacggccca
      601 ctgcatcgtc gcgccagcga tgaccgagtg caggagcaac tcagctgccg caggagcacc
      661 tgggggcagt cgcttgcgga tcccctccac caccgcgcgg ttccgctgga tcgcaagcgt
      721 gcgtagctcc ggcacctgga gctcgtacca ggagatgaga tagttcaccg agaagtcgtt
      781 gcgagtgttc atgctccgaa cgagcacctg caaaaattcc cagagccctt gcggccctgc
      841 gcctatcggt atcgcattca ggtaatgccg cacctgctcg acgccgcgct ccatcatcct
      901 caccagcagc gtatcgcggt tggtgaagcg ctggattaac gctgcgcggg agagccccac
      961 ctcctttgct actccgctga gcgtgaactc tatgggaccg caacgcttca gcactacggt
     1021 ggcggcctcg agtacctcgt catcggactt gagcttgggg cggggcatca gtgttcacct
     1081 tctgtatggg ttggggcgga ggctgtggct gccgccgcca ttgtagcaaa ttgaagacgg
     1141 agcgagagta gagccacgag ccccgcaaac acggccgata caacgaggcc agggagcgga
     1201 ccagcaaggt cgacaaacgc gccggccgca agcataacca tgggcgaggc tgacagcatc
     1261 accgccgaga ccgtgccgag tacccggccg agaagttctg gcggcgtgcg gttgtagatg
     1321 gcagcgttga gaatgggaga gactgagccg gtcagcagtc ccacgagcgc gcccaacaac
     1381 atcagcaccg gcacgcctgg caactgtgaa agcagaagcg agcccaccgc agagccacaa
     1441 aatgccaccg ccagccagtt ctgcgctgat atccgggcgc cgaccgacgc atgaatggca
     1501 atgccaagga gaccaccagc ccccatcatt gaggagaaca gcccgagctc tgctacttgg
     1561 cgtcctgcat ctacaaacag cgcaggcatg atgacgctgc cgttggcgcc aacgatgccc
     1621 acgaagatca tcactatacc aaagagaggg cgcagcaggg gttcgctcca gagaaaagcg
     1681 acgccggcgc gcatggagag agtcgccgtc gtggtcatcg tccgagcggc acgcgcggga
     1741 agcacccacg cgccgagcag acctgcaagg acggagcaga acgccgtcag cccgagcgtt
     1801 ggcgcagcgc caagcaggcc gattgcggcc cccccaaggg ccgggccacc tagaatcgcg
     1861 acgttcccga tcaccgcttt cagtgacgag acgcgctcaa cggagagccc ggcgacgtgg
     1921 ccgagtttgg gcagctcact gtcctgcgcg gccataccgg gtgcgtcgaa cgcggcaccg
     1981 agcaccacgc aagcgatcag cccagtgttc gagagggcgc caacggcatc gagcagtggg
     2041 atgctcgcca tggccacgcc gcccaccaca cccgagatca atgcgacggg cgcgcgcccg
     2101 aaccgatcga cgaggccacc accaacccac gcgccgatga tggtcgcgat gacgctgcta
     2161 gcggccgtgg cgcccgccca ggccgcgctc tttgtatgag acaggacgaa ccatggaagc
     2221 gcgagggccg ccaccgcgtt gccgatccgg aagagaaagg tcgccgcgaa cagcgtcgcg
     2281 agcgggctat atcgacgttc gctcattccg ctgcggcgag ctgcgccttc gccgcagcga
     2341 ggtactcttc gttacccgag tcgagggcga agagtgcgta ggtgaccgcc ccgaacgcaa
     2401 ggcgctccgc gatgtggtgg gcgagccgcg gccacacccg gccaccggcc gcttcatacg
     2461 tgaggaggag cttcgcgagc ccctcttcac caaagaccat aaggtgcgcg gccatgtcga
     2521 tggcagggtc atcaacgcgg gcctcgctcc agtcgatcat cccgctgacg cgctccgtgt
     2581 tgtcgatgag cacatggccc acgtagagat cgccatgcac caccacggag aaatctggcc
     2641 acgacgaatc gtcgtcgagc cagcgctgcc accggtggag gcgcttgtcg ttcaccacga
     2701 actcgcgtcg gacgcggtca acgtcgtcgg ccaccttctg acgggcctgc gtcggtgtac
     2761 ggatgagcat ccccgcatcc acggcggcgg aaatggggac ggcatgcagg gcggcgagcg
     2821 cggtcgcgaa gctctccgcg aagacctccg agtcctgcgg cacgacccag tcgggcgtgg
     2881 acgaaccagg ctggatgacc atcgcagtcg agtcttcgag catgggatag gcaacgagct
     2941 cggcgttggc cacgcgccag tccggcaccg cgaacggcag gcgattcttg agcattgcca
     3001 gcacccgcgc ctctggttcg accttcgcgc ttacctcggc tcggcgcggg atgcgcagca
     3061 cccaccgacg tccatcgtcg acggtggcga tcacgatcct atagtcgagc ccaagctcat
     3121 tgacagtcag cgggccatgg agcttgagcc catgtcgggc tgcaagtgcg tacagttggg
     3181 aggtatcggc ggtcgtgact acggtcatga ttcactcctg agggcttgac gggtttagcc
     3241 acctaaatgt aacagtcacg tcggttatat tcaatccggc actgttgcaa agttagcgat
     3301 gaggcagcct tttgtcttat tcaaaggcct tacatttcaa aaactctgct taccaggcgc
     3361 atttcgccca ggggatcacc ataataaaat gctgaggcct ggcctttgcg tagtgcacgc
     3421 atcacctcaa tacctttgat ggtggcgtaa gccgtcttca tggatttaaa tcccagcgtg
     3481 gcgccgatta tccgtttcag tttgccatga tcgcattcaa tcacgttgtt ccggtactta
     3541 atctgtcggt gttcaacgtc agacgggcac cggccttcgc gtttgagcag agcaagcgcg
     3601 cgaccatagg cgggcgcttt atccgtgttg atgaatcgcg ggatctgcca cttcttcacg
     3661 ttgttgagga ttttacccag aaaccggtat gcagctttgc tgttacgacg ggaggagaga
     3721 taaaaatcga cagtgcggcc ccggctgtcg acggcccggt acagatacgc ccagcggcca
     3781 ttgaccttca cgtaggtttc atccatgtgc cacgggcaaa gatcggaagg gttacgccag
     3841 taccagcgca gccgtttttc catttcaggc gcataacgct gaacccagcg gtaaatcgtg
     3901 gagtgatcga cattcactcc gcgttcagcc agcatctcct gcagctcacg gtaactgatg
     3961 ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa atgccggcct
     4021 ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta tcaccaccga
     4081 ctatttgcaa cagtgcctaa taaggtccct cagggtgggt gctgttctag ttatattgca
     4141 aatcttttat tttttaatag tgagtataat ctctataata gattgaagtc tatgggactt
     4201 tcatattcaa ggcttttaga tgatattact atatcctctg ataaggatct ttcatcagag
     4261 gaaaaaacaa aagtaataaa actcgttcac ggaatggtga atcagtacag actttcaata
     4321 aatgaaagta agacaacgat agagcactca aaagattcgt cttctaaact tagtgtcact
     4381 gggttgtggg taaagcatgg tgttcctaag ttaacaaaag aaaatagacg ttatatcagg
     4441 tacttagttt atatatgtaa aaaacaaggt gcttatgaac gtcatacgaa agagtaccat
     4501 gatttatgga atagatgctc tggcaaggtt gctcaaatgt cgaggcttgg gcatgtccag
     4561 gcggttgaat tgagggcgat tctttctgag ataatgccag tttatgatga ttacaagata
     4621 tctaaattaa agttgatggc taagcactat ctaaataagt ttacgccgcc tttaactgat
     4681 gatcagatta ggaaaataga taggatgctc tatgattttg atatagtagg gcggactaat
     4741 aagaatcttg caaaactata tagacgtaag ctcgtggcat tacttccaga taggtgatta
     4801 tatggaaaat ggatttgtta atttttatga ccatgttaaa gggtatggct ttataaggcg
     4861 tgagcgtgga cgtgatgttt tctttcggta tgatgatttt ttgtttttag ggcatgatgt
     4921 cgatatatgt aaaggtattt tagttaggtt taaattagaa aaaacagata aagggtttaa
     4981 agctgtagcg attcaaaagg tataacatcg tggatgtgtc agcttgtttt tatgatatga
     5041 gaacttgaga tcctttctat ctcgtcgtaa tctcttgctg tgtaaacgaa aaaaccgcct
     5101 tgcagggcgg tttttcgaag gttctcagag ctaccaactc tttgagccga ggtaactggc
     5161 ttggaggagc gcagtcacca aaacttgttc tttcagttta gccttaacag gcgcatgact
     5221 tcaaggctaa ctcctctaat tcggttacca gtggctgctg ccagtggcga taagtcgtgt
     5281 cttctcggtt tggattcaag tcgatagtta ccggataagg cgcagcggtc ggactgaacg
     5341 gggggttcgt gcatacagtc cagcttggag cgaacgacct acaccgggcc gagataccaa
     5401 cagcgtgagc tatgagaaag cgccacgttt cccgaaggga gaaaggcgga caggtatccg
     5461 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5521 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc
     5581 tcgtcagggg ggcggagcct atggaaaaac gccatcggcg cggcctttgt ccctggcttc
     5641 ggctttttgc tcacatgttc tttccggcgt tatcccctga ttctgtggat aaccgtatta
     5701 ccgcctttga gtgagctgac accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
     5761 tgagcgagga agcggaagag cgccttatgt gacattttct ccttacgctc ctctatgccg
     5821 ttctgcatcc tgtccggatg cgttatatcc cggtaagatt ttccgcttca aagcgtgtct
     5881 gtatgctgtt ctgaagttct tccgcgagtt cgtgcagctt ctcacacatg gcggcctgtt
     5941 cgtcagcatc aagtgcgtcc agtttttcga gcagcgtcag gctctggctt tttatgaatc
     6001 ctgccatgtt gagtacggtt tgctgctgct tgttcatctt tctgttttct ccgttctttc
     6061 tgtcatctgc gttgtgtgat tatatcgcgt accacttttc gactgtttta ccgtcggtat
     6121 tctgctgtct gactctttga cgggcatttc tgtcagacaa cactgtcact gccaaaaaac
     6181 tgccgtgcct ttgtcggtaa ttcgagcttg ctgacaggac aggatgtgca attgttatac
     6241 cgcgcataca tgcacgctat tacaattgcc ctggtcaggg ctttgccccg acacccatgt
     6301 cagatacgga gccatgtttt atgacaaaac gaagtggaag taatacgcgc aggcgggcta
     6361 tcagtcgccc tgttcgtctg acggcagaag aagaccagga aatcagaaaa agggctgctg
     6421 aatgcggcaa gaccgtttcc ggttttttac gggcggcagc tctcggtaag aaagttaact
     6481 cactgactga tgatcgggta ctgaaagaag ttatgagact gggggcgtta cagaaaaaac
     6541 tctttatcga cggcaagcgt gtcggggaca gggagtatgc ggaggtgctg atcgctatta
     6601 cggagtatca ccgtgccctg ttatccaggc ttatggcaga ttagcttcca ggggagaaaa
     6661 ctgtcgaaaa cagacggtat gaacactgta agctcccaaa gtgatcacca ctcgctttca
     6721 tgcatagcta tgcagcgagc tgaaaacgat cctgacgcat ttccccggtt ttcttagggt
     6781 aaaacatctc tttttgcagt gtctgtgtca gaatcgcgct cagcgcgatt ctgtggtgcg
     6841 tacaattaag ggattatggt aaatacaatt ggttaaatta ggggtctgac gctcagtgga
     6901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7321 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gcagcagtgt
     7501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7621 cgagttgctc ttgcccggcg tcaacacggg ataataccgc accacatagc agaactttaa
     7681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7861 gggcgacacg aaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7921 accagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7981 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     8041 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaatttt
     8101 ataaaccgtg gagcgggcaa tactgagctg atgagcaatt tccgttgcac cagtgccctt
     8161 ctgatgaagc gtcagcacga cgttcctgtc cacggtacgc ctgcggccaa atttgattcc
     8221 tttcagcttt gcttcctgtc ggccctcatt cgtgcgttct aggatcctcc ggcgttcagc
     8281 ctgtgccaca gccgacagga tggtgaccac catttgcccc atatcaccgt cggtactgat
     8341 cccgtcatca atgaaccgga ctgccacgcc ctgagcgtca aattccttta tcagttggat
     8401 catatcggca gtgtcgcggc caagacggtc gagcttctta accagaatga catcaccttc
     8461 ctccaccttc atcctcagca aatccagccc ttcccggtct gttgaactgc cggatgcctt
     8521 atcggtaaat atacggtttg ctttcacacc tgcgtctttg agtgctctga cctgaagatc
     8581 aagagactgc tgactggttg agacccgagc gtaaccaaaa agtcgcataa aaatgtacct
     8641 taaatcgaat atcggacaac tcatgtctat tattacaaat ttacgattta atagacatat
     8701 taatgtaaca gttttacgat gtccgataat ttataacatt tcgtacggtt ggaaaaatgt
     8761 tactaaatgc ccgtcaggca gggaggccga tatgcccgtt gactttctga ccactgagca
     8821 gactgaaagc tatggcagat tcaccggtga accggatgag cttcagctgg ctctgttgca
     8881 aaaatcgtga agcttgagca tgcttggcgg agattggacg gacggaacga tgacggattt
     8941 caagtggcgc catttccagg gtgatgtgat cctgtgggcg gtgcgctggt attgtcgcta
     9001 tccgatcagc tatcgcgacc ttgaggaaat gctggcggaa cgcggcattt cggtcgacca
     9061 tacgacgatc tatcgctggg tccagtgcta cgccccggag atggagaagc ggctgcgctg
     9121 gttctggcgg cgtggctttg atccgagctg gcgcctggat gaaacctacg tcaaggtgcg
     9181 gggcaagtgg acctacctgt accgggcagt cgacaagcgg ggcgacacga tcgatttcta
     9241 cctgtcgccg acccgcagcg ccaaggcagc gaagcggttc ctgggcaagg ccctgcgagg
     9301 cctgaagcac tgggaaaagc ctgccacgct caataccgac aaagcgccga gctatggtgc
     9361 agcgatcacc gaattgaagc gcgaaggaaa gctggaccgg gagacggccc accggcaggt
     9421 gaagtatctc aataacgtga tcgaggccga tcacggaaag ctcaagatac tgatcaagcc
     9481 ggtgcgcggt ttcaaatcga tccccacggc ctatgccacg atcaagggat tcgaagtcat
     9541 gcgagccctg cgcaaaggac aggctcgccc ctggtgcctg cagcccggca tcaggggcga
     9601 ggtgcgcctt gtggagagag cttttggcat tgggccctcg gcgctgacgg aggccatggg
     9661 catgctcaac caccatttcg cagcagccgc ctgatcggcg cag
//
