LOCUS       NZ_CP051709            12301 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCU-124 plasmid pSCU-124-3, complete
            sequence.
ACCESSION   NZ_CP051709
VERSION     NZ_CP051709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14609784
            Assembly: GCF_012934635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     F plasmids are the primary drivers of antibiotic resistance in
            human-associated commensal E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12301)
  AUTHORS   Stephens,C., Arismendi,T., Wright,M., Gonzalez,A., Gill,M.,
            Hartman,A., Pandori,M. and Hess,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2020) Biology, Santa Clara University, 500 El
            Camino Real, Santa Clara, CA 95054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:52:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,738
            Genes (coding)                    :: 4,482
            CDSs (with protein)               :: 4,482
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 166 of 256
            Pseudo Genes (incomplete)         :: 105 of 256
            Pseudo Genes (internal stop)      :: 30 of 256
            Pseudo Genes (multiple problems)  :: 41 of 256
            Pseudo Genes (short protein)      :: 3 of 256
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCU-124"
                     /isolation_source="rectal swab from healthy college
                     student"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSCU-124-3"
                     /geo_loc_name="USA: California"
                     /collection_date="2016"
     gene            52..243
                     /locus_tag="HHJ37_RS23910"
                     /old_locus_tag="HHJ37_23965"
     CDS             52..243
                     /locus_tag="HHJ37_RS23910"
                     /old_locus_tag="HHJ37_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1446..2312)
                     /locus_tag="HHJ37_RS23915"
                     /old_locus_tag="HHJ37_23970"
     CDS             complement(1446..2312)
                     /locus_tag="HHJ37_RS23915"
                     /old_locus_tag="HHJ37_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(2314..3366)
                     /locus_tag="HHJ37_RS23920"
                     /old_locus_tag="HHJ37_23975"
     CDS             complement(2314..3366)
                     /locus_tag="HHJ37_RS23920"
                     /old_locus_tag="HHJ37_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(3847..4080)
                     /locus_tag="HHJ37_RS23925"
                     /old_locus_tag="HHJ37_23980"
     CDS             complement(3847..4080)
                     /locus_tag="HHJ37_RS23925"
                     /old_locus_tag="HHJ37_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4087..4617)
                     /locus_tag="HHJ37_RS23930"
                     /old_locus_tag="HHJ37_23985"
     CDS             complement(4087..4617)
                     /locus_tag="HHJ37_RS23930"
                     /old_locus_tag="HHJ37_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4655..5086)
                     /locus_tag="HHJ37_RS24540"
                     /pseudo=""
     CDS             complement(<4655..5086)
                     /locus_tag="HHJ37_RS24540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5295..5618)
                     /locus_tag="HHJ37_RS23940"
                     /old_locus_tag="HHJ37_23995"
     CDS             complement(5295..5618)
                     /locus_tag="HHJ37_RS23940"
                     /old_locus_tag="HHJ37_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5682..5873
                     /locus_tag="HHJ37_RS23945"
                     /old_locus_tag="HHJ37_24000"
     CDS             5682..5873
                     /locus_tag="HHJ37_RS23945"
                     /old_locus_tag="HHJ37_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6900..7037)
                     /locus_tag="HHJ37_RS23950"
                     /old_locus_tag="HHJ37_24005"
     CDS             complement(6900..7037)
                     /locus_tag="HHJ37_RS23950"
                     /old_locus_tag="HHJ37_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            7085..7426
                     /locus_tag="HHJ37_RS23955"
                     /old_locus_tag="HHJ37_24010"
     CDS             7085..7426
                     /locus_tag="HHJ37_RS23955"
                     /old_locus_tag="HHJ37_24010"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(7423..8991)
                     /gene="cea"
                     /locus_tag="HHJ37_RS23960"
                     /old_locus_tag="HHJ37_24015"
     CDS             complement(7423..8991)
                     /gene="cea"
                     /locus_tag="HHJ37_RS23960"
                     /old_locus_tag="HHJ37_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            9251..9688
                     /locus_tag="HHJ37_RS23965"
                     /old_locus_tag="HHJ37_24020"
     CDS             9251..9688
                     /locus_tag="HHJ37_RS23965"
                     /old_locus_tag="HHJ37_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            9769..10188
                     /locus_tag="HHJ37_RS23970"
                     /old_locus_tag="HHJ37_24025"
     CDS             9769..10188
                     /locus_tag="HHJ37_RS23970"
                     /old_locus_tag="HHJ37_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(10506..10760)
                     /locus_tag="HHJ37_RS23975"
                     /old_locus_tag="HHJ37_24030"
     CDS             complement(10506..10760)
                     /locus_tag="HHJ37_RS23975"
                     /old_locus_tag="HHJ37_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(10767..11285)
                     /locus_tag="HHJ37_RS23980"
                     /old_locus_tag="HHJ37_24035"
     CDS             complement(10767..11285)
                     /locus_tag="HHJ37_RS23980"
                     /old_locus_tag="HHJ37_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<11323..11754)
                     /locus_tag="HHJ37_RS24545"
                     /pseudo=""
     CDS             complement(<11323..11754)
                     /locus_tag="HHJ37_RS24545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(11963..12286)
                     /locus_tag="HHJ37_RS23990"
                     /old_locus_tag="HHJ37_24045"
     CDS             complement(11963..12286)
                     /locus_tag="HHJ37_RS23990"
                     /old_locus_tag="HHJ37_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(338..419)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(5959..6046)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          451..700
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.7"
                     /coverage="100.0"
                     /evalue="1.47e-93"
     Typing          complement(876..1063)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          6057..6615
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3744..5305)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10403..11973)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.427"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggtatcatt acccccatga acagaacccc cccccttaca cggaggcatc agtgaccaaa
       61 caggaaaaaa ccgcccttaa catggcccgc ttcatcagag gccagacatt aacgctgctg
      121 gagaagctca acgaactgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
      181 cacgccgatg agctttaccg cagttgcctc gcacgcttcg gggatgacgg tgaaaaccgc
      241 tgacacatgc agctcccgga gacggtcaca gcttgtctgt gagcggatgc cgggagcaga
      301 caagcccgcc agggcgcgtc aggccgttga cctttggggt gtcggggcga agccctgacc
      361 cagtcacgta gcgatagcgg agtgtatact ggcttaacca ttcggcatca gtgcggattg
      421 tatgaaaagt gcgccatgtc cggtgtgaaa tgccgcacag atgcgtaagg agaaaatgcc
      481 agttcaggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcggctgc
      541 ggcgagcgga ctctcctttc tcaaaaacgc taaccctgtt atccacagaa cccggggata
      601 aaccaggaaa gaacatgtgc gcaaaaaacg aaaccccggg aaaggccgcg ccggaggcgc
      661 tttttccata ggctccgccc ccctgacgga cttcacaaaa cagacacttt ttcggtgcat
      721 ctgtgggagc cttgcggctc aaccatgaaa tccgccagcg gtggcgaaac ccgacgtggc
      781 ttaaagatca tcaccgtttc cggtgaatcg ctccctcctg cgcccccacg ttcccgccat
      841 atcgattacc ggataccttt tccgcctttc tcccttacgg gacacgtggc gctttctcat
      901 agctcacgtc ttctggtatc tcggggcgac ttaggcagtt cgctccgggc tgggctgtat
      961 gcaggaaccc cccgttcagc ccgactgctg cgccttctcc ggtaactgtt ctcttgggtc
     1021 caacccggaa aagacaccac aaagcgccac tggcagcagc cattggtaaa agtcatgtgc
     1081 gagatattta gagagtactt gaagtggctg ccggagtgcg gctacactag aagaacaatt
     1141 ttggtgactc gtctcgcata agacagttac ctggttaagc agtctgcaag ctgacttaac
     1201 cttcgatcaa accgccctgc aaggcggttt tttcgtttac aggcagcaga ttacgcgcag
     1261 aaaaaaagga tctcaagagg atcctttgat ctttttgtta caaacgtctc tatttgactc
     1321 ccctgagagc gttttagcgc gcctctgacc cccttggtta cattccgaca ccagaaacgc
     1381 tcccgttttt tacgtgcatt tggcacggca tcgatggagg ccggggggca atcctgaaca
     1441 gaatgttact cctctgagta ttttctgtta agaaaatata cacttagcac agttacaata
     1501 aatcctaccc cctgtaagaa tgtgaattgt tcatccaaaa acaaccatga caaaaataat
     1561 gcaactagcg gaataaagta caacattatc cctgccaaat gaactgggag ccaacgcaag
     1621 gagttcaagt aaaaacaaaa agctaaaaaa tattgtaata ttccagatga aaaaacaata
     1681 atcacggatt ccaacggaag ataaattgat gatttagata taaaaataaa gagcgatgac
     1741 agtataaggg agaatgtcaa ctgtccgctc aataatgtga taggctcaaa cgatttaata
     1801 aggcgagacg atataacaac ataaaatgct gccgataaca caccggcaac aacaagacaa
     1861 tatccaacaa tattattcga tccattactg atgtctggca aggctaccaa aatactgcca
     1921 acaattgcac ctataaggaa caatataaaa tattgcgggg aattaatttt aactgagcaa
     1981 ataagaacag acaacaccaa tataaacact gattcaagtg aaaaaataat tgatgcgctt
     2041 cctgctgaaa taaaataaag tccgtagagt gtaaaagtat acgctaagaa tggttcaaaa
     2101 acgcctgtcg cataagcaag tatcgctttt gagtttaatg ggagtttttt tctttttata
     2161 aaaagaacta tccaagatag tattactgca ctacaaatct gccagaaaaa cacccctccg
     2221 gcatctagtt cttcaactcc ttttttgctc attacgatag ctgcccccca tgagaacgct
     2281 gctaaaaaag caaaaagcat tcctttaatc atattaatca tcctctttct cataaagggg
     2341 tttgttttca aatttccttg gttgaaagga gttaaaaaac atagatgcat catctacagg
     2401 aataccaata aactctccat aataaggatc atctatagga aactcttcta atgaaacata
     2461 aaacaataca ttccagttga agatcattgg atcactattt tctatttcat ggagcaatct
     2521 ttcatcctta gttaattctc ctggaattaa tacatcagta tgataaaaac cgctgtaata
     2581 agaactatta ccggtgtgca ctgggcatcc aaaacgatcc aaattagatt ctgaatgcca
     2641 tgttgtagaa atcccttccg gatgtgaata cccaccctta ccaacagact gaatagtcat
     2701 gttacgaata tccgctaacg cttgttcttg atctttatag aaaccttttt ctatcgccag
     2761 cctcataaaa tgccccgggg catttacaga aataagtatt ccccctggta ttcttctatc
     2821 atgatttctt gttctatgag cagaagagta aaaaacatta acaccagcct tatatatttt
     2881 tagcccatct ttatcctgaa atggtacgct ttcagtataa ataacatcct cttttattgg
     2941 atagcactct ggcatatgca gcgaacaaat atatttttgt atgtccacta actcttctga
     3001 tggacgagca aaggcaagtt gtggatgaat aaaataaatg agatgcgcac tgacctctcc
     3061 tcttcttgcg cgttctttaa aacgaactct ttcattacgc aaaaaatcaa acaaatgacc
     3121 atcatcatta tatagttggg ggctatttac aatagataaa tggaaatcaa gccctttaat
     3181 tttcttgctt gccaatttac caaatacaca gggctgattg cctgacaccc attttcttag
     3241 taaatcttca attttatctt tagaagtttc gttaaagata aatgaatgaa tattatcaat
     3301 atcttttcca aatgaaaaac tggatgataa ttgtggtagt ttatttatac gttgttgaag
     3361 attcatgaaa gctcccacaa gacacctcat atattttttt atatactgaa actacattat
     3421 atttattacc ggcgtaaaac gaaataattt attatcgtta ccgatatttt aaaaaaatca
     3481 ttatttgcac agtgttcgag ttgttagtat cactccttcc tgaaaatccg ctccatttac
     3541 cataacgtcg cattgtacgc accgccaaaa cgcgttcggt gcgatcacgg cagcagacag
     3601 gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcacctgata aattttaacc
     3661 gcatgaatac ctatgcaacc agatgctaca ggccacatta gcccgcctta atccactgaa
     3721 cacgtcattt ttcatggttt caccatccca gcgaagggcc atccagtgtc cgctcctgga
     3781 tttccggctg acgctcccgc tccagagata actcatgttc gcgttcctgc atcagccgtt
     3841 catcccttat ctcctgttct cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa
     3901 gtgctgcacc tgctgactca actgcatgac tcgcccgttc agcatcgcgt tgtcccgttg
     3961 cgtaagcgaa aacattttct gcaattccac gaaggcgttc tcccattcgt tcagccgctg
     4021 catatagtcc tgttgcagtt gctctaatgc gttcagcaaa tgtctttcca gctccgtcat
     4081 gctctgtcac tcctgcacca gtctcactgc gttcagtttg ccgttgtcct tcgtccagtt
     4141 cgtttcggct ttcatcccct tcggcaacac cagaaaacgt ccgttgctgt cctcgtgata
     4201 acgcacgccc catgtcttcg cgttcagctt ctccagactc tctttctgct cgcggagaat
     4261 attcatattc gcgtttatct tcatgccctg gtaccacagc agactcccgg tcgtggcaaa
     4321 catcagtact gagacgataa tcagccacag ccagcgtttc acgctcattt tcagtacggt
     4381 tttccagtgc gcgtccatcg tatcgacggt cgttttctga tacctctcca gcagttcttt
     4441 cagggctgtt gtatggtcgt tgatagcgtt cctgattaaa gattcgtttg aggtcagttc
     4501 cctgtttaca gattcttcga gcttcctgaa ctcgtcgttc agcattttct ctgtagattc
     4561 ggctctctct ttcagctttt tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa
     4621 acgctccttt cagtctgata ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc
     4681 gcgtcaccct gaccacttca agccccgcct ctgtgagcgc ctgaatcaca tcctgacggc
     4741 cttttatctc cccgacatga taaagagcgt ctatcccgcg tgtgacgctc tctgcaagcg
     4801 cctgtttcgt ttccggcagg ttatcagaga gagtcagtat ccggcggttc tccggggcgt
     4861 tggggtcatg cagcccgtaa tggtgattca ccagcgtctg ccaggcatca attctcggcc
     4921 tgtctgcccg gtcgtagtac ggctggaggc gttttccggt ctgtagctcc atgttcggaa
     4981 tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact
     5041 gatttttttc gagaccgggc atcagtacac gctcaaagct cgccatcacc ttttcacgtc
     5101 ctcccggtgg cagctccttc tccgcgaacg acagcacacc ggacgtgtat ttcttcgcaa
     5161 atggcgtggc atcgatgagt tcccgtactt cttccggatt accctgaagc accgttgccc
     5221 cttcccggtt acgctccctt cccagcaggt aatcaaccgg accactgcca ccaccttttc
     5281 cccgggcatg aaacttaact atcatcccgc gccccctgtt ccctgacggc cagacgcagc
     5341 cggcgcagct catccccgat ggccatcagt gcggccacca cctgaacccg gtcaccggaa
     5401 gaccactgcc cgctgttcac cttacgggct gtctgattca ggttatttcc gatggcggcc
     5461 agctgacgca gtaacggcgg tgccagtgtc ggcagttttc cggaacgggc aaccggctcc
     5521 cccaggcaga cccgccgcat ccataccgcc agttgtttac cctcacagcg ttcaagtaac
     5581 cgggcatgtt catcatcagt aacccgtatt gtgagcatcc tctcgcgttt catcggtatc
     5641 attaccccat gaacagaaat cccccttaca cggaggcatc agtgactaaa caggaaaaaa
     5701 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgctgctg gagaagctca
     5761 acgaactgga cgcagatgaa caggccgata tttgtgaatc gcttcacgac cacgccgatg
     5821 agctttaccg cagctgcctc gcacgtttcg gggatgacgg tgaaaacctc tgatacatgc
     5881 agctcccgga gacggtcaca gcttgtctgt gagcggatgc cgggagctga caagcccgtc
     5941 agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc cctgacccag tcacgtagcg
     6001 atagcggagt gtatactggc ttaaccatgc ggcatcagtg cggattgtat gaaaagtacg
     6061 ccatgccggg tgtgaaatgc cgcacagatg cgtaaggaga aaatgcacgt ccaggcgctt
     6121 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtact
     6181 gactcacaca aaaacggtaa cacagttatc cacagaatca ggggataagg ccggaaagaa
     6241 catgtgagca aaagaccagg aacaggaaga aggccacgta gcaggcgttt ttccataggc
     6301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6481 ctcatagctc acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6601 agtccaaccc ggtaaggcac gccttaacgc cactggcagc agccactggt aaccggatta
     6661 gcagagcgat gatggcacaa acggtgctac agagttcttg aagtagtggc ccgactacgg
     6721 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6781 aagagttggt agctcttgat ccggcaaaca aaccaccgtt ggtagcggtg gtttttttgt
     6841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6901 tactgaaccg cgatccccgt cagtttagaa gaggaggatg gtgcgatggt ccctccctga
     6961 acatcacgta tatagttagc ctgacatcca acaaggaggt ttatcgcgaa tattcccaca
     7021 aaaaatcttt tcctcataac tcgatcctta taaaatgaaa agaatatatg gcgaggttta
     7081 atttatgagc ttaagatact acataaaaaa tattttattt ggcctgtact gcacacttat
     7141 atatatatac cttataacaa aaaacagcga agggtattat ttccttgtgt cagataagat
     7201 gctatatgca atagtgataa gcactattct atgtccatat tcaaaatatg ctattgaaca
     7261 catagctttt aacttcataa agaaagattt tttcgaaaga agaaaaaacc taaataacgc
     7321 ccccgtagca aaattaaacc tatttatgct atataatcta ctttgtttgg tcctagcaat
     7381 cccatttgga ttgctaggac tttttatatc aataaagaat aattaaatcc ctaacacctc
     7441 atttatagta ttaagtttat tcttatcaat ataggagcat agaattcctg taacaatagc
     7501 aataccccaa atacctaatg tagttccagc aagcaagcta aaaagtaaag caacaacata
     7561 actcacccct gcatctgcag ctttcttctc taatgtaaga aatagtggct tccagtcacc
     7621 tgtatcctta atttttagga tatcagatac cacatcatat ccaaaagaaa catgccccgt
     7681 aatcttcaag tacttggcaa actgatctaa atgtttagcc cagtcatcat acttcaccga
     7741 tgccaacgca ttaaaaatag catcacgatc ggctttgctg aatttcttat ttaaaacatc
     7801 cttgtatttt tcaaaagcag cgagagcttc attcacattg ccgattttct tacctttaga
     7861 cttatcagca agttcctgtg ccattttcga atatttttca ccatattttt cagtcagcgt
     7921 ttgataaaag ctaactgttg catcaacagc atccttaatc tgtgaattaa ggagattatt
     7981 ctgtgctttt ttcaaatttt cttcagcttc atgaacacga gcgataccgg cattacgatt
     8041 attactgacc tgagaaatag ccttctggat ctgagttata tcagcattta tccggttaat
     8101 acgtgtttct gatgctgtta cctgtttttg tttttcttct ctaatcttac cggccccaac
     8161 ccgtcgtctg gttgcttcaa aaaaaggacg gttctgaagc ggatcattgg ctcttggtga
     8221 tagttttttg accagctcat ccagttcttt atatttagcg gatgcctgag ccagttcatt
     8281 tcgttttcca gcgagcgttt tcatttctgc atcacgggca tggatactgg agcttaaacg
     8341 agaattgaga gtcttaatct ctccatccat tttcaccact tcagattgtg cagcagaaag
     8401 ttttttttgg gcgatctcaa cagctttagc ttcttcactc aatgcagcca gtcgtttctc
     8461 ttcagcttca gccagtttca actggcgttc tgtttcagcc ttctcccgtt caatctcttt
     8521 acgtcgttgt tctgcttcct gaaaagcctt ttctgctgct tccgcttctt tacgggcttt
     8581 ttcttctgct ttcgcaaggc gcaaacgctc tgcttccgcc tgcatagctg cattattagc
     8641 atgagcaagc tctgttgctg aaggcgtacg tgaggcattg tgacgaagag cctcattcac
     8701 gatatccttc aggcgctgag tcagcgcatc cctgtttgcc tttgctttcg cctgtgcttc
     8761 cgctgcagct tttgcccggg cagcctgctc tgcctgtgtt ttctttaatt gagcagtaga
     8821 ccatttagca gttgcatgaa tagctgcaga actttcactt ttactgcctc cttttccacc
     8881 tccgccgcca gagccactcc cgtcaggagt accattcaaa agagtaataa ttacctgtcc
     8941 cttatcatca taaggaacac catctttata gtacgctacc gcggtttcca ttataaaatc
     9001 ctctttgact tttaaaacaa taagttaaaa ataaatactg tacatataac cactggtttt
     9061 atatacagca taaaagctac gccgctgcat tttccctgtc aagactgtgg acttccattt
     9121 ttgtgaaaac gatcaaaaaa acagtctttc acaccacgcg ctattctcgc ccgatgccac
     9181 aaaaaccagc acaaacatta ccgttctcag acctcattat gttttactga aactatgaga
     9241 tgagacatct atgggacact gtcactttat ggcatggcac acactccggg acgcactaaa
     9301 aatgacaggc agatcgcgtt cacagtttta ccgtgatatg cgcgcaggcc ttgtcagtta
     9361 ccgtaccggc agggacggac gacgggagtt tgaaaccagt gaactgatcc gggcatacgg
     9421 cgaattaaag cagaatgaga caccagaaag gcacagtgag ggacatgcag aaaatccaca
     9481 tgatcagcag acagaacgca ttctccggga gctgaatgag ctgaaacaat gcctgacgct
     9541 gatgcttgag gataaacagg cacaggatat ggatcgcaga cgccaggaag cagaacggga
     9601 acagctacaa aatgagatag cccagctcag gcaggcactg gaactggaaa agaaacgggg
     9661 attctggtcc aggttgttcg gtcgctgaac gctgtcagag actgatgata aaatagtctt
     9721 cggataataa ctcaccgaga ataaatactt taaggtaggg agacactcat gagacgtacc
     9781 ggaaacaaac tttgtcttat cgccatgata acagcaacag tagctctcac agcctgtacc
     9841 ccaaagggca gcgtggaaca acatacccgg cattacgtat atgcttctga tgacggtttt
     9901 gatcccaact tttccaccca aaaagccgac acaacacgaa tgatggtgcc tttttttcgg
     9961 cagttctggg atatgggagc taaagataaa gcgacaggaa aatcacggag tgatgtgcaa
    10021 caacgcattc agcagtttca cagccaggaa tttttaaact cactccgggg cacaactcaa
    10081 tttgcgggta ctgattaccg cagcaaagac cttaccccga aaaaatccag gctgctggct
    10141 gacacgattt ctgcggttta tctcgatggc tacgagggca gacagtaagt ggatttacca
    10201 taatccctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc agcagacagg
    10261 taaaaatggc aacaaaccac ccgaaaaact gccgcgatcg cgcctgataa attttaaccg
    10321 tatgaatacc tatgcaacca gagggtacag gccacattac ccccacttaa tccactgaag
    10381 ctgccatttt tcatggtttc accatcccag cgaagggcca tccagcgtgc gttcctgtat
    10441 ttccggctga cgctcccgtt ccagggataa cacatgttcg cgctcctgta tcagccgttc
    10501 ctctcttatc tccagttctc gctgtataac tggctcaagc gttctgtctg ctcgctcaag
    10561 cgttctgtct gctcgctcaa gtgctgcacc tgctgactca actgcatgac ccgctcgttc
    10621 agcatcgcgt tgtcccgttg cgtaagcgaa aacatcttct gcaattccac gaaggcgctc
    10681 tcccattcgc tcagccgctg catatagtcc tgttgcagct gctctaaggc gttcagcaaa
    10741 tgtgtttcca gctctgtcac tctgtgtcac tccttcagat gtacccactc tttcccctga
    10801 aagggaatca cctccgctga tttcccgtac ggaaggacaa ggaatttcct gttcccgtcc
    10861 tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct gcaatgtctc ttcctgcttc
    10921 ctgatttctt ccaggttcgc ctgtatcctc cctccaagat accagagcgt cccgccactc
    10981 gcggtaaaca ggagaaatac tatccccagt aacatcatgc ccgtattccc tgccagcttt
    11041 aacacgtccc tcctgtgctg catcatcgcc tctttcaccc cttcccggtg tttttccagc
    11101 gattcctctg tcgaggctgt gaacagggct atagcgtctc tgattttcgt ctcgtttgat
    11161 gtcacagcct cgcttacaga ttcgccgagc ctcctgaact cgttgttcag cattttctct
    11221 gtagattcgg ctctctcttt cagctttttc tcgaactccg cgcccgtctg caaaagattg
    11281 ctcataaaac gctcctttca gtctgatatt cctcccgccg ttcgggtctg caatgctgat
    11341 actgcttcgc gtcaccctga ccacttcaag ccccgcctct gtgagcgcct gaatcacatc
    11401 ctgacggcct tttatctctc cggcatggta aagcgcgtct atcccgcgtg tgacgccctc
    11461 agcaagcgcc tgtttcgttt ccggcaggtt atcaggaagg gtcagcgtcc ggcggttctc
    11521 cggggcgttg gggtcatgca gaccgtaatg gtgattcacc agcgtctgcc aggcatcaat
    11581 tctcggcctg tctgcccggt cgtagtacgg ctggaggcgt tttccggtct gtagctccat
    11641 gttcggaatg acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat
    11701 gctgtactga tttttctcga gaccgggcat cagtacacgc tcaaagctcg ccatcacttt
    11761 ttcacgccct ccaggcggca gctccttctc cgcgaacgac agaacacctg acgtgtattt
    11821 cttcgcaaat ggcgtggcat cgatgagttc ccggacctct tccggattcc cctgaagcac
    11881 cgttgcccct tcccggttac gctcccttcc cagcaggtaa tcaaccggac cactgccacc
    11941 accttttccc ctggcatgaa acttaactat catcccgcgc cccctgttcc ctgaccgcca
    12001 gacgcagccg gcgcagctca tccccgatgg ccatcagtgc ggccaccacc tgaacccggt
    12061 caccggaaga ccactgcccg ctgttcacct tcctcgctgt ctggttaagg ttattcccga
    12121 tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg cagcttcccg gaacgggcaa
    12181 ccggctcacc caggcagacc cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt
    12241 ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tcgcgtttca
    12301 t
//
