LOCUS       NZ_CP051617             8554 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli O25b:H4-ST131 strain 2017_APHA plasmid
            pAPHA_2017_2, complete sequence.
ACCESSION   NZ_CP051617
VERSION     NZ_CP051617.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14594834
            Assembly: GCF_014217155.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Duggett,N.A., Randall,L., Lemma,F., Teale,C. and Anjum,M.F.
  TITLE     First detection of the pandemic Escherichia coli ST131 blaCTX-M-27
            fimH30(R) human clone in pigs from United Kingdom
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Duggett,N.A., Randall,L., Lemma,F., Teale,C. and Anjum,M.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2020) Bacteriology, Animal and Plant Health
            Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,042
            CDSs (total)                      :: 4,932
            Genes (coding)                    :: 4,717
            CDSs (with protein)               :: 4,717
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 77 of 215
            Pseudo Genes (incomplete)         :: 162 of 215
            Pseudo Genes (internal stop)      :: 35 of 215
            Pseudo Genes (multiple problems)  :: 52 of 215
            Pseudo Genes (short protein)      :: 3 of 215
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /strain="2017_APHA"
                     /isolation_source="cecal sample"
                     /host="Pig"
                     /db_xref="taxon:941322"
                     /plasmid="pAPHA_2017_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /genotype="ST131"
     gene            join(7354..8554,1..689)
                     /gene="cya"
                     /locus_tag="HHM36_RS24805"
                     /old_locus_tag="HHM36_24820"
     CDS             join(7354..8554,1..689)
                     /gene="cya"
                     /locus_tag="HHM36_RS24805"
                     /old_locus_tag="HHM36_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_106372970.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKAAAEAKAKAEAEKARKEAEAKANNEKAVLTKASEIIISVGDKVGEYLGDK
                     YKALSREIAGNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVAL
                     AIFSATLGAYLLAVGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            complement(717..1241)
                     /gene="cui"
                     /locus_tag="HHM36_RS24810"
                     /old_locus_tag="HHM36_24825"
     CDS             complement(717..1241)
                     /gene="cui"
                     /locus_tag="HHM36_RS24810"
                     /old_locus_tag="HHM36_24825"
                     /inference="COORDINATES: protein motif:HMM:NF015795.2"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            1361..1501
                     /gene="crl"
                     /locus_tag="HHM36_RS24815"
                     /old_locus_tag="HHM36_24830"
     CDS             1361..1501
                     /gene="crl"
                     /locus_tag="HHM36_RS24815"
                     /old_locus_tag="HHM36_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            complement(2526..2717)
                     /locus_tag="HHM36_RS24820"
                     /old_locus_tag="HHM36_24840"
     CDS             complement(2526..2717)
                     /locus_tag="HHM36_RS24820"
                     /old_locus_tag="HHM36_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2782..3105
                     /locus_tag="HHM36_RS24825"
                     /old_locus_tag="HHM36_24845"
     CDS             2782..3105
                     /locus_tag="HHM36_RS24825"
                     /old_locus_tag="HHM36_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3314..>3745
                     /locus_tag="HHM36_RS25340"
                     /pseudo=""
     CDS             3314..>3745
                     /locus_tag="HHM36_RS25340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3783..4313
                     /locus_tag="HHM36_RS24835"
                     /old_locus_tag="HHM36_24855"
     CDS             3783..4313
                     /locus_tag="HHM36_RS24835"
                     /old_locus_tag="HHM36_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            4320..4553
                     /locus_tag="HHM36_RS24840"
                     /old_locus_tag="HHM36_24860"
     CDS             4320..4553
                     /locus_tag="HHM36_RS24840"
                     /old_locus_tag="HHM36_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6182..6610)
                     /locus_tag="HHM36_RS24845"
                     /old_locus_tag="HHM36_24865"
     CDS             complement(6182..6610)
                     /locus_tag="HHM36_RS24845"
                     /old_locus_tag="HHM36_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6691..7128)
                     /locus_tag="HHM36_RS24850"
                     /old_locus_tag="HHM36_24870"
     CDS             complement(6691..7128)
                     /locus_tag="HHM36_RS24850"
                     /old_locus_tag="HHM36_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     Typing          2354..2441
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(1785..2328)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.689"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3096..4636
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.05"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggcgaaagc tgaagctgaa aaagcggctg ctgaagcaaa agcaaaagct gaagctgaaa
       61 aagccagaaa ggaagctgaa gcaaaagcaa ataacgagaa agctgttctg acaaaagcca
      121 gtgaaattat cattagtgtg ggtgataagg tcggagaata tcttggcgat aaatataaag
      181 ctctttctcg tgagatagca ggtaatatca aaaactttca aggtaagacg atccgtagtt
      241 atgatgaggc gatggcttct gtcaataaac tgatggctaa tcctgatctt aaaattaatg
      301 ctgcagacag ggatgtcatt gtgaatgcct ggaaagcatt tgatgcagag gatatgggga
      361 ataagtttgc cgcgctgggt aagacattta aagccgcaga ttatgtgatg aaggcaaata
      421 acgtccggga gaagagtata gagggatatc agaccggaaa ctgggggccg ttaatgcggg
      481 aggtcgagtc ctgggttgtc agtggtattg cttctgctgt ggctcttgct attttttctg
      541 ccacccttgg agcttacctg cttgctgtgg gggcatcagc tgcagtcgta gggattatcg
      601 gtattattat agccagtttt attggagcat tgattgatga caaatttata gacaggttaa
      661 ataacgaaat tattcgccct gcgtattaac ctagagtcga aaggtagtca agaagactac
      721 ctttctttcc agagtttata tgacacaacc ggaatataac acagagcata agtcataaag
      781 aaagagcaaa aatagaggca ggcgtaaaaa ataaacaaag taaaatcatt atcggacatc
      841 catctgacag tcctacctgc agtggttaat tcgaaattat tccacagaaa gaaatatgca
      901 taaaaagcat aaaaaaatgg actaaatatg ctggcagtaa ttagtttttc tctgttggct
      961 attgtctcta gctttctgtc gcggagttgg agagtaaaag caataagtgc cagtaaggga
     1021 gcgcttttcc cgtagatatc catcaccttt gtcatcacag gattgtatgc ggaaattatt
     1081 gaaggtatat tttgtgtgtt atcaaaaatg atgctaatta aatagaactt ggggtttgat
     1141 gtataagtta taaaaataat gattaatggt gttatgcctg caaaagtaat caaaaaaagc
     1201 gtcctgttta aatctttgtt gtcagtgtgc tcttttctca ttggtataac tctccaagct
     1261 tttcattatt attaatttag ttgtagatgt gtataagcat atcatgctgt acaagtaatg
     1321 gtcatcctaa gattaggggt atgtgttaat gagggaatat atgaaaatga tattatcagg
     1381 agggatgctt gtcttgatgg ctttgctatc aggctgccaa gctaattaca tccgcgatgt
     1441 tcagggaggg actgtcgcac catcctcctc ttctaaactg acggggatcg cggttcagta
     1501 gaaaagttaa aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa
     1561 acgaaaaaac cgcctgggga ggcggtttga tcgaaggtta ggtcagttgg ggaactgctt
     1621 aaccaaggta actggcttca gcagagcgca gataccaaat actgtccttt cagtgtagcc
     1681 gtagtcgggc cactacttca agaactctgt agcaccgttt gtgccatcat cgctctgcta
     1741 atccggttac cagtggctgc tgccagtggc gttaaggcgt gccttaccgg gttggactca
     1801 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1861 cccagcttgg agcgaacgac ctacaccaag ccgagatacc aacagcgtga gctatgagaa
     1921 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1981 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2041 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2101 ctatggaaaa acgccagcgg cgcggccttt cgttggcctt tgcctttttg ctcacatgtt
     2161 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgcctttg agtgcgccgg
     2221 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2281 gtgtcggaag ttgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2341 cacttttcat acaatccgca ctgatgccgc atagttaagc cagtatacac tccgctatcg
     2401 ctacgtgact gggtcagggc tgcgccccga cacccgctaa aacctgctga cgcgccctga
     2461 cgggcttgtc tgctctcggc atccattcac agaccagttg tgtacgtctc cgggagctgc
     2521 atgtgtcagt ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctca
     2581 tcggcgtggt cgtgaagcga ttcacagata tctgcctgtt catccgcgtc cagttcgttg
     2641 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     2701 ttttcctgtt ttgtcactga tgcctccgtg taagggggat ttccgttcat gggggtaatg
     2761 ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     2821 ttactggaac gctgtgaggg taaacaactg gcggtatgga tgcggcgggt ctgcctgggt
     2881 gagccggttg cccgttccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     2941 gccgccatcg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3001 tccggtgacc gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg
     3061 ctgcgtctgg ccgtcaggga acaggggacg cgggatgata gttaagtttc atgccagggg
     3121 aaaaagtggt ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg
     3181 ggcaacggtg cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt
     3241 tgcgaagaaa tacacgtcag gtgttctgtc gttcgcggag aaggagctgc cgccgggagg
     3301 acgtgaaaaa gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca
     3361 gtacagcatc ctgtgggtgg agcaccagga caagggacgg cttgagctga attttgtcat
     3421 tccgaacatg gagctacaga gcggaaaacg cctccagccg tactacgacc gggcagacag
     3481 gccgagaatt gatgcctggc agacgctggt gaatcaccat tacggtctgc atgaccccaa
     3541 cgccccggag aaccgccgga cgctgaccct tcctgataac ctgccggaaa cgaaacaggc
     3601 gcttgctgag ggcgtcacac gcgggataga cgcgctttac catgccggag agataaaagg
     3661 ccgtcaggat gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg
     3721 aagcagtatc agcattgcag acccgaacgg cgggaggaat atcagactga aaggagcgtt
     3781 ttatgagcaa tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg
     3841 aatctacaga gaaaatgctg aacgacgagt tcaggaagct cgaagaatct gtaaacaggg
     3901 aactgacctc aaacgaatct ttaatcagga acgctatcaa cgaccataca acagccctga
     3961 aagaactgct ggagagatat cagaaaacga ccatcagcac gatggataca cactggcaaa
     4021 ccgtactgaa aatgagcgtg aaacgctggc tgtggctgat tatcgtctca gtgctgatgt
     4081 ttgccacgac cgggagtctg ctgtggtatc agggcatgaa gataaatgca aacatgaata
     4141 ttctccgcga gcagaaagag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt
     4201 atcacgagga cagcaacggg cgttttcttg tgttgccgaa ggggatgaaa gccgaaacga
     4261 actggacgga ggacaacggc aaactgaatg cagtgagact ggtacaggag tgacagagca
     4321 tgacggagct ggaaaaacat ttgctgaacg cattagagca actgcaacag gactatatgc
     4381 agcggctgaa cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc
     4441 aacgggacaa cgcgatgctg aacgagcggg tcatgaagtt gagtcagcag gtgcagcact
     4501 tgagcgagcg gacagagcgc ttgagccagt tatacagcga gaacaggaga taagggacga
     4561 acggctgatg caggaacgcg aacatgagtt atctctggaa cgggagcgga agctggatgg
     4621 cccttcgctg ggatggtgac tgaaagtccg gttgagtcgt aaacagaata ttttccagaa
     4681 atgtcaggct gcctattggt aatataacca gtcgttcttg cggtttattg atttttgtac
     4741 aggttgttgc tgtttttttt gtaaagagga caggtatttt tctgaaaatg aacttgcggg
     4801 aatgcgcggg ttatgtaaaa aatataattg cgtatctccg atatgtttgt aataccgatg
     4861 tggacacagc gaatctggaa ggggtcggag atgcgcgttc tctcatgtgt taatattgag
     4921 ttcttacatg tggttgcata ttttttttgc atatttgaag tctgcgaatt atattctgat
     4981 atgcaaaagt caggactaga catactatcc atcgattcag tccttacctt acgccctcac
     5041 tgatttcctg ccgtgagggc ttttttcatg ggtgtgaaaa aaggaagccc cacctgacgg
     5101 aggggctgag tgatacagag caaaaaaata tcctggtgtt ggtcacctct ggagagtaat
     5161 aagattaacc ataaaacaaa ctataaacaa gaatattgat gccaggaatt ttcctggtta
     5221 ttttttttgt tttttacaaa aaagacaaaa taacagaaag taaaatctga tcatttttgt
     5281 tgattgttat tgctctgtac cgttattctt aagctgaaag tgaattcttc taattttttc
     5341 ttatattcag acgcaattaa cagtccctgc tccttgaggg actttttttt gtctttttct
     5401 ctctgtttta tattgttttg agtagtaact ataaaggcag tgaagttctg tcttatgagc
     5461 gcgtttctgg cggtgatgcg actaacccat tctacatcaa cgaacaattc accagaaacg
     5521 cgcctgtaaa tttttagctg agcaagggct ctgtgtcgag agttgtggat gtctggttcg
     5581 ccattttaaa aaacttcttc cggcggtttt tagtttcgat gcaggtcagg gggcgctgta
     5641 aagttatgtt ccctgattac tgtgccagaa tcctgagaca gacaggaggt ctaccggtta
     5701 tttttcgaat tgggcgcttc cggaagagag agtttcattg ggcgttggtc gcggatggtg
     5761 agcacgtttc gatgttgtgt gacgaacatt cagctcagtt gatggagact gtcagaaacg
     5821 cgctcagaag tgttgtaacg cgatttctgg tatcaggcaa ttgtttcctt tttctggagc
     5881 atgatacctg tgtgtacggt gatgagttct gtcacgtaca gtaatatttc cctcctgatg
     5941 cttccatctg gcaggagttg aacctgccga aaatatgaaa aaacccccgt ttcagttaca
     6001 tcaggcaggc aattcgttcg tgattcgcat ggctgcatag atatgcatga ggttaaaatt
     6061 tatcaggtgc gatcgcggcg gtttttcggg tggtttgttg tcatttttac ctgtctgctg
     6121 ccgtgatcgc gctgaacgca tttcagtggt gcgtacaatt aagggattat ggtaaatcca
     6181 tttactgtct tccctcgtag ccatcgagat aaaccgcaga aatcgtgtca gccagtagct
     6241 tcattttttc cggtgatggc atatcactgt tcgtatattt tctgcccgca aaggtggttg
     6301 tgcttctgag agactgaaga aatttttctg agtggaattc ttttatgcgt tgctgtgccg
     6361 tttctgctga tactccggct gctttgtctt tctcccccat ctccctgaac tgctgaaaga
     6421 acggaatcat catccgtgtg gtgtcggcct tgttggtgta gaagttaggg tcagaacggt
     6481 catcggaagc gtacacataa tgtcgggtat attgttccac actgcctttc ggggtacagg
     6541 ccgtgagggc tgcagccaca gttatcaggg atataagata cagatagcgt gtgccaccag
     6601 aacgtttcat acgtgtctcc ttaccttaaa gtatttattc tcggtgagtt attatccgaa
     6661 tactatctta tcatcagcct ctgaccgagt tcagtgaccg aagagtctgg accagaatcc
     6721 ccgtttcttt tcctgttcca gtgcctgcct gagctgggct atctcattct gtaactgttc
     6781 ccgttctgat tcctggtgcc tgcgatccat atcctgtgcc tgtttatcct caagcatcag
     6841 cgtcaggcat tgtttcagct cattcagttc ccggagaatg cgttctgtct gctgatcatg
     6901 tggattttct gcctgtccca cagtgtgcct ttcttgtgtc tcattttgct ttaattcgcc
     6961 gtatgcccgg atcagttcac tggtttcaaa ctcccttcgt ccgtcctggc tggtacggta
     7021 actgacaaga cctgagcgca tatcacggta aaactgtgaa cgcgatcttc cagtcatttt
     7081 cagtgcgtcc cggagtgtgt gccatgccat aaagttacag tgtcccatag gtgtctcatc
     7141 tcatggtttc agtaaaacat aatgaggtct gagaacggta atgtttgcgc cggtttttgt
     7201 ggtatccggc gggaatagcg cgtggtgtga gagactgttt ttttgatcgt tttcacaaaa
     7261 atagaagtcc acagtcttga cagggaaaat gcagcggcgt agcttttgta ctgtacataa
     7321 aaccagtggt tatgtataca gtatttgttt ttaatggttt gttttaaaag tcaaagagga
     7381 atgaatatgc cggggtttaa ttacggtggg tatggtgacg gtactggctg gagttctgaa
     7441 agtggtggtc cagctccagg cggtggtatg catggaaata gtggaggcca gcgcggagat
     7501 aacgcaaaca gtagcaattc tgtgagtcag cagatttctg ccattcagaa tgatcagaaa
     7561 cttaaacaga aagtggtgaa tatgctgatt gctgcgagaa aaatgaatcc tgaagcaaaa
     7621 atgattctgg gaagtattgc tccttctggt gttatgcagg tcaccattga ggggataacc
     7681 tccactcagg ccaagcagct tggtttggga gggctggtca tgggatataa cgcttctggt
     7741 gttattgggg ctgtgggcga aatagacacc ggtcaccgcc ttaatgcatc aggagcaagt
     7801 actcccggaa gcgaaacttc tgtggagagt tttgtgaacg gacagaaacc cgccggagaa
     7861 tggcatgctg tggcgaagga cagctggaca ggggctggtc cagtgaatgt agggctggtt
     7921 aataacgcca tcaaaagtgt ccggattatc aaaaaaggct atgtgactgg tgttctcttg
     7981 cctgaagagg tgatgaataa agcagagtac aaagcaatgc ggcaggcttt cgattccctt
     8041 ccactggcaa aacagggaga ggctgtcaga cagattgttg cagcctggag tctggcatat
     8101 caggatttcc cggtaaatct gaaaaaagat atggggcgag ttactgaacg ggtggtggat
     8161 gcggttaatt tggcgctcat tctcaaccag atatcatccg gaatgtcggc ctcacagaag
     8221 gatgttgatg cggcgaaccg gattattaat gagaccgtga aagccattaa cgacgtaaat
     8281 cttaaaatag cggagaagaa gaaacaacag gtaccattat taagccttat gaaacaaaaa
     8341 cagaaagagg ttgaagaact aaaaaaagta tttaagaacc attcttatca ccgtattcgt
     8401 gatgctcaga gagcatatga tgatgcccgg aacaaaaatg atttactggt cagtgatatt
     8461 aatgcgctgc aggcacaggt ttccggactg actgcaagga aacagcaggc agaaaagaac
     8521 aaggcggcag cagaaaaagc aaaagctgat gcaa
//
