LOCUS       NZ_CP051421             8496 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Uganda strain CVM 20723
            plasmid p20723-4, complete sequence.
ACCESSION   NZ_CP051421
VERSION     NZ_CP051421.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14504525
            Assembly: GCF_016454725.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Uganda
  ORGANISM  Salmonella enterica subsp. enterica serovar Uganda
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Zhao,S., Li,C., Hsu,C.H., Tyson,G.H., Strain,E., Tate,H., Tran,T.T.,
            Abbott,J. and McDermott,P.F.
  TITLE     Comparative Genomic Analysis of 450 Strains of Salmonellaenterica
            Isolated from Diseased Animals
  JOURNAL   Genes (Basel) 11 (9), 1025 (2020)
   PUBMED   32883017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, United States of America
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 448x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016454725.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 02:49:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,974
            CDSs (total)                      :: 4,853
            Genes (coding)                    :: 4,717
            CDSs (with protein)               :: 4,717
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 61 of 136
            Pseudo Genes (incomplete)         :: 97 of 136
            Pseudo Genes (internal stop)      :: 26 of 136
            Pseudo Genes (multiple problems)  :: 41 of 136
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051421.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Salmonella enterica subsp. enterica serovar
                     Uganda"
                     /mol_type="genomic DNA"
                     /strain="CVM 20723"
                     /serovar="Uganda"
                     /isolation_source="bovine"
                     /sub_species="enterica"
                     /db_xref="taxon:487004"
                     /plasmid="p20723-4"
                     /geo_loc_name="USA:TN"
                     /collection_date="2002"
                     /collected_by="TN State Veterinary Diagnostic Lab"
     gene            complement(33..644)
                     /locus_tag="HFQ25_RS00520"
                     /old_locus_tag="HFQ25_00515"
     CDS             complement(33..644)
                     /locus_tag="HFQ25_RS00520"
                     /old_locus_tag="HFQ25_00515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000130481.1"
                     /translation="MTDKTTDIKMALENMIRFRDTIERLGGSMAKEFDVETKGKRTYSM
                     NNDMSDLYNVISRAVEASGADHSINEKLTNVLKRELVDYVSIAHLKTKLSVLYEFEKNY
                     LQLIAEYKEEIKFASSLQEDLRKERAKFFSETLKEVHQTLNESQVDNEVASKWIKELVG
                     SYTKSLDLSGGLVEEHTLDTIACIRAEAKLNKPSIEPGNN"
                     /name="hypothetical protein"
     gene            complement(977..1210)
                     /locus_tag="HFQ25_RS00525"
                     /old_locus_tag="HFQ25_00520"
     CDS             complement(977..1210)
                     /locus_tag="HFQ25_RS00525"
                     /old_locus_tag="HFQ25_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001427885.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1217..1735)
                     /locus_tag="HFQ25_RS00530"
                     /old_locus_tag="HFQ25_00525"
     CDS             complement(1217..1735)
                     /locus_tag="HFQ25_RS00530"
                     /old_locus_tag="HFQ25_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023167411.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTTSTEESLKKHREGVKEAMMQHRKDVLKLAGNTGVMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGNRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1773..2435)
                     /locus_tag="HFQ25_RS24740"
                     /old_locus_tag="HFQ25_00530"
                     /pseudo=""
     CDS             complement(<1773..2435)
                     /locus_tag="HFQ25_RS24740"
                     /old_locus_tag="HFQ25_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2413..2736)
                     /locus_tag="HFQ25_RS00540"
                     /old_locus_tag="HFQ25_00535"
     CDS             complement(2413..2736)
                     /locus_tag="HFQ25_RS00540"
                     /old_locus_tag="HFQ25_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955989.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRICLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2801..2992
                     /locus_tag="HFQ25_RS00545"
                     /old_locus_tag="HFQ25_00540"
     CDS             2801..2992
                     /locus_tag="HFQ25_RS00545"
                     /old_locus_tag="HFQ25_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165990.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGGDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3652..3810
                     /locus_tag="HFQ25_RS25125"
     CDS             3652..3810
                     /locus_tag="HFQ25_RS25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            4100..5068
                     /locus_tag="HFQ25_RS00550"
                     /old_locus_tag="HFQ25_00550"
     CDS             4100..5068
                     /locus_tag="HFQ25_RS00550"
                     /old_locus_tag="HFQ25_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_013815101.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIT"
                     /name="IS5 family transposase"
     gene            5241..6056
                     /locus_tag="HFQ25_RS00555"
                     /old_locus_tag="HFQ25_00555"
     CDS             5241..6056
                     /locus_tag="HFQ25_RS00555"
                     /old_locus_tag="HFQ25_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_013815100.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYRWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAAFLRRLHSIPVCNCPFNSDHVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFHKYGIDNPDMNKLQFHLMLDEFF"
                     /name="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
     gene            6107..7075
                     /locus_tag="HFQ25_RS00560"
                     /old_locus_tag="HFQ25_00560"
     CDS             6107..7075
                     /locus_tag="HFQ25_RS00560"
                     /old_locus_tag="HFQ25_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_013815099.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(7197..8000)
                     /locus_tag="HFQ25_RS00565"
                     /old_locus_tag="HFQ25_00565"
     CDS             complement(7197..8000)
                     /locus_tag="HFQ25_RS00565"
                     /old_locus_tag="HFQ25_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_013815098.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKESE
                     HKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQEWR
                     KSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYKRLQ
                     DEATEEAMNDLMRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIVSLNQ
                     SIDRLTNQGQKFIESHLERFHIVDATSSLYITGDEKERLLLKNIN"
                     /name="hypothetical protein"
     gene            complement(7987..8463)
                     /locus_tag="HFQ25_RS00515"
                     /old_locus_tag="HFQ25_00510"
     CDS             complement(7987..8463)
                     /locus_tag="HFQ25_RS00515"
                     /old_locus_tag="HFQ25_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244589582.1"
                     /translation="MNDFKLSSSELPEILAALREARECLVSRSINEDPQLYSALFLYEK
                     NEGLLSLSFEREYSIFINSNLECILRKVSDIKDKKQGKLQKIMNEFVRFKYSSGDSCAD
                     LLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKERVIFEREFERLLGEKNGDF"
                     /name="hypothetical protein"
     ARG             5242..6053
                     /name="aph(3')-Ia"
                     /gene="aph(3')-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.52"
                     /coverage="100.0"
     Typing          complement(3071..3155)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          3179..3724
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.255"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(874..2423)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaactattt atatgtctac ccccggccat aattagttat tgcccggctc aatagatggt
       61 ttatttaatt tagcctcagc gcgaatacaa gcaattgtat ctaatgtatg ctcttcaaca
      121 aggcctccgc ttagatccaa gcttttggta taactaccaa caagttcttt aatccatttt
      181 gatgccactt cattatcaac ttgagattca tttagagttt gatgaacctc ttttaatgtc
      241 tcagaaaaga atttagcacg ttctttacgt aaatcctctt gcaaagagga agcaaatttt
      301 atttcttcct tatattctgc gataagctgc aaataattct tttcaaactc atataataca
      361 gacaatttgg tttttagatg cgcaatgctg acataatcaa ctaattctct tttcaagaca
      421 tttgtcaatt tttcattaat tgaatgatcg gcgccagacg cttcaacagc ccgtgatatt
      481 acattatata aatcactcat atcgttattc atagaataag tacgttttcc tttggtctca
      541 acatcaaact ctttcgccat agaacctccc aatctctcta tagtgtcacg aaatctgatc
      601 atgttttcta aagccatttt aatatcggtc gttttatctg tcatgactta ggcctaactc
      661 atctatttac cataacgtcg cattacgcgc accgctaaaa cgcgttcagc acgatcacgg
      721 cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcgcctgata
      781 aattttaacc gcatgaatac ctatgcaacc agatggtaca ggccacatta acccctctta
      841 atccactgaa gctgccattt ttcatggttt caccatccca gcgaaggacc atccagcgtg
      901 cgttcctgta tttccggctg acgctcccgt tccagggata atgcatgttc gcgctcctgt
      961 atcagccgtt cctctcttat ctccagttct cgctgtataa ctggctcaag cgttctgtct
     1021 gctcgctcaa gtgctgcacc tgctgactca actgcatgac ccgctcgttc agcatcgcgt
     1081 tgtcccgttg cgtaagcgaa aacatcttct gcaattccac gaaggcgctc tcccattcgt
     1141 tcagccgctg catatagtcc tgttgcagtt gctctaatgc gttcagcaaa tgtctttcca
     1201 gctccgtcac tctgtgtcac tccgtcagtt gtacccagtc cttcccctga tagggaatca
     1261 ccgttgccga tttcccctgc gggatgacca gaaatttcct gttcccgtcc tgcacaaact
     1321 ccacgcccca tgtcttcgcg ttcagtttct gcaatgtctc ttcctgtatc ctgatttctt
     1381 ctaggttcgc ctgtatccgc ccgccaagat accagagcgt cccgccactc gcggtaaaca
     1441 ggagaaagac tatccccagt aacatcacgc ccgtattccc tgccagcttt aacacgtcct
     1501 tccggtgctg catcatcgcc tctttcaccc cttcccggtg ttttttcagc gattcctctg
     1561 tcgacgttgt gaacagggct atagcgtctc tgattttcgt ctcgtttgat gtcacagcct
     1621 cgcttacaga ttcgccgagc ctcctgaact cgttgttcag cattttctct gtagattcgg
     1681 ctctctcttt cagctttttc tcgaactccg cgcccgtctg taaaagattg ctcataaaat
     1741 gctcctttca gcctgatatt cctcccgccg ttcgggtctg caatgctgat actgcttcgc
     1801 gtcaccctga ccacttcaat ccctgcctcc gtgagcgcct gaatcacatc ctgacggcct
     1861 tttatctccc cgacatgata aagagcgtct atcccgcgtg tgacgctctc agcaagcgcc
     1921 tgtttcgttt ccggcaggtt atcagagaga gtcagtatcc ggcggttctc cggggcgttg
     1981 gggtcatgca gcccgtaatg gtgattcacc agtgtctgcc aggcatcaat tctcggcctg
     2041 tctgcccggt cgtagtacgg ctggaggcgt tttccggtct gtagctccat attcggaatg
     2101 acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat gctgtactga
     2161 tttttttcga gaccgggcat cagtacacgc tcaaagctcg ccatcacttt ttcacgccct
     2221 cccggcggca gctccttctc cgcgaacgac agaacacctg acgtgtattt cttcgcaaat
     2281 ggcgtggcat cgatgagttc ccggacttct tccggatcac cctgaagcac cgttgcccct
     2341 tcccggttac gctcccttcc cagcaggtaa tcaaccggac cactgccacc gccttttccc
     2401 ctggcatgaa acttaactat catcccgcgc tccatgttcc ctgacgacct gccgtaagct
     2461 gcgcaactct ctctcgatgg ccatcagtgc ggccaccacc tgaacccggt caccggaaga
     2521 ccactgcccg ctgttcacct tcctcgccgt ctgattcagg ttattcccga tggcagccag
     2581 ctggcgcagt aacggcggtg ccagtgtcgg cagcttcccg gaacgggcaa ccggctcacc
     2641 caggcagatc cgccgcatcc ataccgccag ctgtttaccc tcacagcgtt ccagtaaccg
     2701 tgcatgttca tcatcagtaa cccgtatcgt gagcatcctc tcgcgtttca tcggtatcat
     2761 taccccatga acagaaatcc ccccttacac ggaggcatca gtgactaaac aggaaaaaac
     2821 cgcccttaac atggcccgct ttataagaag ccagacatta acgctgctgg agaagctcaa
     2881 cgaactggac gcagatgaac aggccgatat ttgtgaatca cttcacgacc acgccgacga
     2941 gctttaccgc agctgcctcg cacgcttcgg gggtgacggt gaaaacctct gacacataca
     3001 gctcccggag acggtcacag cttgtctgtg agcggatgcc gggagcagac aagcccgtca
     3061 gggcgcgtca gggggtttcg gggcgaagcc ctgaaccagt cacgtagcga tagcggagtg
     3121 tatactggct taaccatgcg gcatcagtgc agattgtatg aaaagtgcgc catgccgggt
     3181 gtgaaatgcc gcacagatgc gtaaggagaa aatgcagctt ccgacgctct tccgcttcct
     3241 cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3301 aggcggtaat acggttatcc acagaatcag gggataacgc cggaaagaac atgtgcgcaa
     3361 aaggcaaagg ccgggagaaa ggccacgccg atggcgtttt tccataggct ccgcccccct
     3421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3601 cgctgttggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3721 gtaagacacg acaaagcgcc actggcagca gccactggta actggacaag gtggattgag
     3781 atattaagag ttcttgaagt ggtggcctaa ctgcggctac actagaagga cagaatttgg
     3841 tatctgtgct ccaccaagcc agttaccttc ggaagaaaga gttggtagct ctagattccg
     3901 gcaaacaaac caccgttggt agcggtggtt tttttgttta caggcagcag attacgcgca
     3961 gaaaaaaagg atcacaagag gatccttgaa tcttttcact cccactccta ggtcatagcc
     4021 atagcgctca gttttcaacc acaagacaaa aactaatgat tagcaacggc tttgttgaat
     4081 aaatcgaact tttgctgagt tgaaggatca gatcacgtat cttcccgaca acgcagaccg
     4141 ttccgtggca aagcaaaagt tcaaaatcac caactggccc acctacaata aagccctcat
     4201 caaccgtggc tccataactt tctggctgga tgatgaagct attcaggcct ggtatgagtc
     4261 agcaacacct tcttcacgag gcagacctca gcgctattct gaccttgcca tcacgactgt
     4321 gctggtcatt aaacgcgtgt tcaggttgac cctgcgggct gcacagggtt ttattgattc
     4381 catttttaca ctgatgaatg ttccgttgcg ctgcccggat tacaccagtg tcagcaagcg
     4441 cgcaaagtcg gttaatgtca gtttcaaaac gttcacccgg ggtgaaatcg cgcatctggt
     4501 gattgattcc accgggctga aggtcttcgg tgaaggcgag tggaaagtca aaaagcatgg
     4561 ccaggaacgc cgccgtatct ggcgtaagct gcatctcgcc gttgacagta aaacacatga
     4621 aatcatctgc gctgacctgt cgctgaacaa tgtgacggac tcagaagcct tcccgggtct
     4681 tatccggcag actcacagaa aaatcagggc agcatcggca gacggcgctt acgacacccg
     4741 gctctgtcac gatgaactgc ggcgtaagaa aatcagcgcg cttatcccac cccgaaaagg
     4801 tgcgggttac tggcccggtg aatatgcaga ccgtaaccgt gcagtggcta atcagcgaat
     4861 gaccgggagt aatgcgcggt ggaaatggac aacagattac aaccgtcgct cgatagcgga
     4921 aacggcgatg taccgggtaa aacagctgtt cgggggttca ctgacgctgc gtgactacga
     4981 tggtcaggtt gcggaggcta tggccctggt acgagcgctg aacaaaatga cgaaagcagg
     5041 tatgcctgaa agcgtgcgta ttacctgaaa acacaacccg ctacggggga gacttacccg
     5101 aaatctgatt tattcaacaa agccatggcg ggaaccacat tgtgtctcaa aatctctgat
     5161 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     5221 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagaccgc
     5281 gattaaattc caacctggat gctgatttat atgggtatag atgggctcgc gataatgtcg
     5341 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
     5401 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     5461 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     5521 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc
     5581 ctgattcagg tgaaaatatt gttgatgcgc tggcagcgtt cctgcgccgg ttgcattcga
     5641 ttcctgtttg taattgtcct tttaacagcg atcacgtatt tcgtctcgct caggcgcaat
     5701 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     5761 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
     5821 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     5881 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     5941 actgcctcgg tgagttttca ccttcattac agaaacggct ttttcataaa tatggcattg
     6001 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     6061 aattggttaa ttggggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     6121 cacgtatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     6181 ctggcccacc tacaataaag ccctcatcaa ccgtggctcc ataactttct ggctggatga
     6241 tgaagctatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg
     6301 ctattctgac cttgccatca cgactgtgct ggtcattaaa cgcgtattca ggctgaccct
     6361 gcgcgctgcg cagggcttta ttgattccat tttttctctg atgaacgttc cgctacgctg
     6421 cccggattac agctgtgtca gcaggcgggc aaagtcggtt aatgtcagtt tcaaaacgcc
     6481 cacccggggt gaaatcgcac acctggtaat tgattccacc gggctgaagg tcttcggtga
     6541 aggcgagtgg aaagtcaaaa agcatggcca ggaacgccgc cgtatctggc gtaagctgca
     6601 tctcgccgtt gacagtaaaa cacatgaaat catctgcgct gacctgtcgc tgaacaatgt
     6661 gacggactca gaagccttcc cgggtcttat ccggcagact cacagaaaaa tcagggcagc
     6721 atcggcagac ggcgcttacg acacccggct ctgtcacgat gaactgcggc gtaagaaaat
     6781 cagcgcgctt atccctcccc gaaaaggtgc gggttactgg cccggtgaat atgcagaccg
     6841 taaccgtgca gtggctaatc agcgaatgac cgggagtaat gcgcggtgga aatggacaac
     6901 agattacaac cgtcgctcga tagcggaaac ggcgatgtac cgggtaaaac agctgttcgg
     6961 ggggtcactg acgctgcgtg actacgatgg tcaggttgcg gaggctatgg ccctggtacg
     7021 agcgctgaac aaaatgacga aagcaggtat gcctgaaagc gtgcgtattg cctgaaaaca
     7081 caacccgcta cgggggagac ttacccgaaa tctgatttat tcaacaaagc cattagcaac
     7141 cgcaagaaca aacaatgcga ccattaaaaa taatgatcgc tattactcac aaaatactaa
     7201 ttaatatttt tcagcaacaa tcgttctttc tcatcacctg taatatataa agaagatgtc
     7261 gcatcaacaa tatgaaaacg ctccaaatga ctttcaatga atttttgtcc ttgattagtc
     7321 agcctatcaa tactttggtt taaactaaca atatctctat ttaattcttc caaaaaccga
     7381 gttgacgtat caataacatt ccccatctta gtatcaactg cacgaattaa aatatcttta
     7441 gctctttcat tatcaccaaa cttttcctca atcctcataa gatcattcat agcctcttca
     7501 gttgcttcat cttgcaggcg cttatattgt acagctttct catagacaaa tttctgtgcg
     7561 cgctccttta aatcaagctg catgttccca atagagttaa tcacttctat atttaatttc
     7621 gttaattgct ccttgtaacg tgcaatagct gctgttgttc gctccatcct ctcaagttcc
     7681 tgtgactttc tccattcctg gatctcagct actgcttttt ctatttcgcg aacgcgacga
     7741 acctctagat caacttcaag agttttctct ttttctctca gctcatattc agcattcaat
     7801 tcactcattc tgagttcatg ttcaacacgt ttcttggccg tcaagacatc catgttgcgc
     7861 ttatgttcag actcctttga agcctcctct cttctatatt ccctatcttt caatggttct
     7921 ttagcccatt cacagagtgc atcaacaggc ttactaatac aattaagcaa agcgctacca
     7981 atctgactaa aaatccccat ttttttcacc taaaagccgt tcaaactctc tttcaaaaat
     8041 aacacgttct ttcttcatca tatagttaag aaattcaagt ctatttctat ccctatcaaa
     8101 gacttcatat attttacgta tctgttcaag tttaccactc aaaaacaaaa gatccgcaca
     8161 agaatcgccg cttgaatatt taaacctcac aaattcattc attatttttt gcagtttacc
     8221 ctgcttttta tcttttatat cagaaacttt cctcaatatg cactctaaat tggagttaat
     8281 aaaaatagag tattctctct caaatgaaag acttagcaac ccttcatttt tttcatataa
     8341 aaataatgcc gaatataact gaggatcttc attgatgctg cgcgatacaa ggcattccct
     8401 tgcctctcgt aaagcagcca atatctcagg aagttccgac gaagacaatt taaagtcatt
     8461 cactttacgt tctaatatag ccacaagaac ataaag
//
