LOCUS       NZ_CP051417            10910 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Muenchen strain CVM
            20744 plasmid p20744-1, complete sequence.
ACCESSION   NZ_CP051417
VERSION     NZ_CP051417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14504546
            Assembly: GCF_016454705.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Muenchen
  ORGANISM  Salmonella enterica subsp. enterica serovar Muenchen
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Zhao,S., Li,C., Hsu,C.H., Tyson,G.H., Strain,E., Tate,H., Tran,T.T.,
            Abbott,J. and McDermott,P.F.
  TITLE     Comparative Genomic Analysis of 450 Strains of Salmonellaenterica
            Isolated from Diseased Animals
  JOURNAL   Genes (Basel) 11 (9), 1025 (2020)
   PUBMED   32883017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, United States of America
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 481x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016454705.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 03:00:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,635
            Genes (coding)                    :: 4,518
            CDSs (with protein)               :: 4,518
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 52 of 117
            Pseudo Genes (incomplete)         :: 83 of 117
            Pseudo Genes (internal stop)      :: 19 of 117
            Pseudo Genes (multiple problems)  :: 32 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Salmonella enterica subsp. enterica serovar
                     Muenchen"
                     /mol_type="genomic DNA"
                     /strain="CVM 20744"
                     /serovar="Muenchen"
                     /isolation_source="swine"
                     /sub_species="enterica"
                     /db_xref="taxon:596"
                     /plasmid="p20744-1"
                     /geo_loc_name="USA:NC"
                     /collection_date="2002"
                     /collected_by="NC State Veterinary Diagnostic Lab"
     gene            join(10883..10910,1..629)
                     /locus_tag="HFQ44_RS23905"
     CDS             join(10883..10910,1..629)
                     /locus_tag="HFQ44_RS23905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244664296.1"
                     /translation="MVGPCLAGLLKPCLGLERFGLLCPALLRGLAVVPLEPLSGGPLDA
                     VIDPLGDHQVAVRVLAATGMDGQRIRQALGTGQVLGELGRQRLLLVEGQLDRQGKFDLL
                     EQPPIGAFIQVGSIPVVGGPLDELRHVPGFGVQDFIHVAGILAFALDVVGLGPGRLAAR
                     PAAGFRRKVINRHAASLLLLLFGSMQWPSESAPPEGWPLGQFLEEKPVSAPSPTK"
                     /name="hypothetical protein"
     gene            699..983
                     /locus_tag="HFQ44_RS23585"
                     /old_locus_tag="HFQ44_23590"
     CDS             699..983
                     /locus_tag="HFQ44_RS23585"
                     /old_locus_tag="HFQ44_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            1581..>1769
                     /locus_tag="HFQ44_RS23590"
                     /old_locus_tag="HFQ44_23595"
                     /pseudo=""
     CDS             1581..>1769
                     /locus_tag="HFQ44_RS23590"
                     /old_locus_tag="HFQ44_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(1736..2935)
                     /gene="tet(A)"
                     /locus_tag="HFQ44_RS23595"
                     /old_locus_tag="HFQ44_23600"
     ARG             complement(1740..2935)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3041..3691
                     /gene="tetR(A)"
                     /locus_tag="HFQ44_RS23600"
                     /old_locus_tag="HFQ44_23605"
     CDS             3041..3691
                     /gene="tetR(A)"
                     /locus_tag="HFQ44_RS23600"
                     /old_locus_tag="HFQ44_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(3723..3965)
                     /locus_tag="HFQ44_RS23605"
                     /old_locus_tag="HFQ44_23610"
     CDS             complement(3723..3965)
                     /locus_tag="HFQ44_RS23605"
                     /old_locus_tag="HFQ44_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4271..5107)
                     /locus_tag="HFQ44_RS23610"
                     /old_locus_tag="HFQ44_23620"
     CDS             complement(4271..5107)
                     /locus_tag="HFQ44_RS23610"
                     /old_locus_tag="HFQ44_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(5108..5526)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(5111..5910)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5971..6786)
                     /gene="sul2"
                     /locus_tag="HFQ44_RS23620"
                     /old_locus_tag="HFQ44_23630"
     ARG             complement(5975..6786)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7093..7944)
                     /gene="repC"
                     /locus_tag="HFQ44_RS23625"
                     /old_locus_tag="HFQ44_23640"
     CDS             complement(7093..7944)
                     /gene="repC"
                     /locus_tag="HFQ44_RS23625"
                     /old_locus_tag="HFQ44_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(7931..8770)
                     /locus_tag="HFQ44_RS23630"
                     /old_locus_tag="HFQ44_23645"
     CDS             complement(7931..8770)
                     /locus_tag="HFQ44_RS23630"
                     /old_locus_tag="HFQ44_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(8800..9006)
                     /gene="urfF"
                     /locus_tag="HFQ44_RS23635"
                     /old_locus_tag="HFQ44_23650"
     CDS             complement(8800..9006)
                     /gene="urfF"
                     /locus_tag="HFQ44_RS23635"
                     /old_locus_tag="HFQ44_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(9008..9220)
                     /locus_tag="HFQ44_RS23640"
                     /old_locus_tag="HFQ44_23655"
     CDS             complement(9008..9220)
                     /locus_tag="HFQ44_RS23640"
                     /old_locus_tag="HFQ44_23655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(9281..10252)
                     /locus_tag="HFQ44_RS23910"
     CDS             complement(9281..10252)
                     /locus_tag="HFQ44_RS23910"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_010890158.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(10249..10662)
                     /locus_tag="HFQ44_RS23915"
     CDS             complement(10249..10662)
                     /locus_tag="HFQ44_RS23915"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     ARG             complement(4275..5107)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(590..745)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(7932..8753)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(9285..10909)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcaagccatg ccttgggctt gagcgcttcg gtcttctgtg ccccgccctt ctccggggtc
       61 ttgccgttgt accgcttgaa ccactgagcg gcgggccgct cgatgccgtc attgatccgc
      121 tcggagatca tcaggtggca gtgcgggttc tcgccgccac cggcatggat ggccagcgta
      181 tacggcaggc gctcggcacc ggtcaggtgc tgggcgaact cggacgccag cgccttctgc
      241 tggtcgaggg tcagctcgac cggcagggca aattcgacct ccttgaacag ccgcccattg
      301 gcgcgttcat acaggtcggc agcatcccag tagtcggcgg gccgctcgac gaactccggc
      361 atgtgcccgg attcggcgtg caagacttca tccatgtcgc gggcatactt gccttcgcgc
      421 tggatgtagt cggccttggc cctggccgat tggccgcccg acctgctgcc ggttttcgcc
      481 gtaaggtgat aaatcgccat gctgcctcgc tgttgctttt gcttttcggc tccatgcaat
      541 ggccctcgga gagcgcaccg cccgaagggt ggccgttagg ccagtttctc gaagagaaac
      601 cggtaagtgc gccctcccct acaaagtagg gtcgggattg ccgccgctgt gcctccatga
      661 tagcctacga gacagcacat taacaatggg gtgtcaagat ggttaagggg agcaacaagg
      721 cggcggatcg gctggccaag ctcgaagaac aacgagcgcg aatcaatgcc gaaattcagc
      781 gggtgcgggc aagggaacag cagcaagagc gcaagaacga aacaaggcgc aaggtgctgg
      841 tgggggccat gattttggcc aaggtgaaca gcagcgagtg gccggaggat cggctcatgg
      901 cggcaatgga tgcgtacctt gaacgcgacc acgaccgcgc cttgttcggt ctgccgccac
      961 gccagaagga tgagccgggc tgaatgatcg accgagacag gccctgcggg gctgcacacg
     1021 cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg gctaaaagcg ctccagcgta
     1081 tttctgcggg gtttggtgtg gggtttagcg ggctttgccc gcctttcccc ctgccgcgca
     1141 gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca gctatccggc ctctggccgg
     1201 gcatattggg caagggcagc agcgccccac aagggcgctg ataaccgcgc ctagtggatt
     1261 attcttagat aatcatggat ggatttttcc aacaccccgc cagcccccgc ccctgctggg
     1321 tttgcaggtt tgggggcgtg acagttattg caggggttcg tgacagttat tgcagggggg
     1381 cgtgacagtt attgcagggg ggcgtgacag ttattgcagg ggggcgtgac agttattgca
     1441 ggggggcgtg acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc
     1501 aggcatttcg gctgagggta aaagaacttt ccgctaagcg atagactgta tgtaaacaca
     1561 gtattgcaag gacgcggaac atgcctcatg tggcggccag gacggccagc cgggatcggg
     1621 atactggtcg ttaccagagc caccgacccg agcaaaccct tctctatcag atcgttgacg
     1681 agtattaccc ggcattcgct gcgcttatgg cagagcaggg aaaggaattg ccgggctatg
     1741 tgcaacggga atttgaagaa tttctccaaa gcccgcgacg cagcgccggc aggcagagca
     1801 agtagagggc agcgcctgca atccatgccc acccgttcca cgttgttata gaagccgcat
     1861 agatcgccgt gaagaggagg ggtccgacga tcgaggtcag gctggtgagc gccgccagtg
     1921 agccttgcag ctgcccctga cgttcctcat ccacctgcct ggacaacatt gcttgcagcg
     1981 ccggcattcc gatgccaccc gaagcaagca ggaccatgat cgggaacgcc atccatcccc
     2041 gtgtcgcgaa ggcaagcagg atgtagcctg tgccgtcggc aatcattccg agcatgagtg
     2101 cccgcctttc gccgagccgg gcggctacag ggccggtgat cattgcctgg gcgagtgaat
     2161 gcagaatgcc aaatgcggca agcgaaatgc cgatcgtggt cgcgtcccag tgaaagcgat
     2221 cctcgccgaa aatgacccaa agcgcggccg gcacctgtcc gacaagttgc atgatgaaga
     2281 agaccgccat cagggcggcg acgacggtca tgccccgggc ccaccggaac gaagcgagcg
     2341 ggttgagagc ctcccggcgt aacggccggc gttcgccttt gtgcgactcc ggcaaaagga
     2401 aacagcccgt caggaaattg aggccgttca aggctgccgc ggcgaagaac ggagcgtggg
     2461 gggagaaacc gcccatcagc ccaccgagca caggtcccgc gaccatcccg aacccgaaac
     2521 aggcgctcat gaagccgaag tgccgcgcgc gctcatcgcc atcagtgata tcggcaatat
     2581 aagcgccggc taccgcccca gtcgccccgg tgatgccggc cacgatccgc ccgatataga
     2641 gaacccaaag gaaaggcgcc gtcgccatga tggcgtagtc gacagcagcg ccggccagcg
     2701 agacgagcaa gaccggccgc cgcccgaaac gatccgacag cgcgcccagc acaggtgcgc
     2761 aggcaaattg catcaacgca tacagcgcca gcagaatgcc atagtgggcg gtgacgtcgt
     2821 tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg cataatcagg ccgatgccga
     2881 cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg tctgttgggt ttcacgtctg
     2941 gcctccggac cagcctccgc tggtccgatt gaacgcgcgg attctttatc actgataagt
     3001 tggtggacat attatgttta tcagtgataa agtgtcaagc atgacaaagt tgcagccgaa
     3061 tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc ggcgtagacg gtctgacgac
     3121 acgcaaactg gcggaacggt tgggggttca gcagccggcg ctttactggc acttcaggaa
     3181 caagcgggcg ctgctcgacg cactggccga agccatgctg gcggagaatc atacgcattc
     3241 ggtgccgaga gccgacgacg actggcgctc atttctgatc gggaatgccc gcagcttcag
     3301 gcaggcgctg ctcgcctacc gcgatggcgc gcgcatccat gccggcacgc gaccgggcgc
     3361 accgcagatg gaaacggccg acgcgcagct tcgcttcctc tgcgaggcgg gtttttcggc
     3421 cggggacgcc gtcaatgcgc tgatgacaat cagctacttc actgttgggg ccgtgcttga
     3481 ggagcaggcc ggcgacagcg atgccggcga gcgcggcggc accgttgaac aggctccgct
     3541 ctcgccgctg ttgcgggccg cgatagacgc cttcgacgaa gccggtccgg acgcagcgtt
     3601 cgagcaggga ctcgcggtga ttgtcgatgg attggcgaaa aggaggctcg ttgtcaggaa
     3661 cgttgaagga ccgagaaagg gtgacgattg atcaggaccg ctgccggagc gcaacccact
     3721 cactacagca gagccatgta gacaacatcc cctccccctt tccaccgcgt cagacgcccg
     3781 tagcagcccg ctacgggctt tttcatgccc tgccctagcg tccaagcctc acggccgcgc
     3841 tcggcctctc tggcggcctt ctggcgctcc tgctgcggcg tccgctcgtg ggccgtggcg
     3901 cgggtccgcg cgccggcctc gtgcgcctgg cgctcgcggg cgaggtccag ggcggccgtc
     3961 ttcacgttct gccttgcgca gatgagatag atctagcgtg gactcaaggc tctcgcgaat
     4021 ggctcgcgtt ggaaactttc attgacactt gaggggcacc gcagggaaat tctcgtcctt
     4081 gcgagaaccg gctatgtcgt gctgcgcatc gagcctgcgc ccttggcttg tctcgcccct
     4141 ctccgcgtcg ctacggggct tccaggggtc gtttgcggga aggggcggaa tcctacgcta
     4201 aggctttggc cagcgatatt ctccggtgag attgatgtgt tcccagggga taggagaagt
     4261 cgcttgatat ctagtatgac gtctgtcgca cctgcttgat cgcggccgcg atagctagat
     4321 cgcgttgctc ctcttctcca tccgcgttcc aagctgcgga aaggcatcca taagcgtacg
     4381 cctggtcgag caggcgacgc ggatcgacgt ccagcgcacg agagaatgcg tccgccatct
     4441 gtgcaatgcg tctaggatcg agacaaaggt cgtctctgtc agccggatcg tagaacatat
     4501 tggcggcgcc aaagcccact tcaccgacca gaccgacggg atctatcacc agccagccgc
     4561 gactggagaa catgatgttt tcatgatgca gatcgccatg tagcccacgc agttccgagg
     4621 cattgctcat catttgatcg gctataatcg ccgcgtggac gtagtcagtt tgacaacctg
     4681 cgttttgatc atcgcgcgcc cgctgaaaca aagctgcaaa gcgatcccgg atcgggagaa
     4741 gggcagaagg caggggttcc tcagatgcgg catacagctt cgccattagt tccgctgcaa
     4801 tttcggtcgc ctggtagtcg ccgtgctcgg caacgatgtg agagagcatt cgctccccgg
     4861 catattcgag caacatcaga ttgttctcac gaccgagcaa ccggactgct cccctcccat
     4921 tgcgccatac cagatagtcg gccccgcgca gttcatcagc aatgtcttct ataggtttca
     4981 atcccttgac gattgcagga gtcccgtctg gcaatgaaac tttccaaacg aggctggaaa
     5041 aggtgtccgc aatgagaaca ggttgcgaaa cgtgccaatg agcaggaaaa acaggcggca
     5101 tgaacatcaa ccccaagtca gagggtccaa tcgcagatag aaggcaaggc gttcgcggtc
     5161 gggggcttcg atccccaata cattgaatag gacagcgaag gcgcgctctg cttcatctgg
     5221 cgctgcccag ttctcttcgg cgttagcaat catgagtgcc aaatcggcat agcgatctgc
     5281 tgttccgagc cgcccaaggt cgatcagacc cgtgcattga agagttttag ggtccaccat
     5341 gaagttcggc atgcagggat caccatggca aacaaccata tcggtgcgct cttggtcgag
     5401 ccgcaccggt agctctcgtt cgacacgagc caaaagatcg agctgcggcg tactcttgtc
     5461 ctcgtccggt aagaagtcgg gattgacggc attgcgggac accacatcaa cggcgcgtcc
     5521 gaacattcgc gacagcctgc gctcaaacgg acattgatca accgataggc tgtgaacagc
     5581 gccaagttgc tgccccattg acggccacgc tttgagcaaa tccgctccag acagatcagc
     5641 cgccggtact cccggaattg ccgttatcac caagcatgca ccctcctgtt cctcctgcca
     5701 gttgatgacc tcggggcaag ccacacctcg acctttgagc caaatgaggc ggtcacgctc
     5761 tccagcgagc tcaccgcggc gggaagcagg tgcgattttc gcgaaggcat gcccgtcacc
     5821 acgtcgaaaa acaaaatcac cagattctcc gcctctgaca ggcaaccagt cagaatgcga
     5881 ttcaccaaaa aaaatattag ttcgattcaa tggaggttcc ttcagttttc tgatgaagcg
     5941 cgaatataga gaaatatccc gaatgtgcag ttaacgaatt cttgcggttt ctttcagcgc
     6001 cgccaatacc gccagcccgt cgcgcaaggg gcgcggctcg tgtgtgcgga tgaagtcagc
     6061 tccacctgcg gcggcggcaa gctctgcagc gagtgtcgcg gccccgacat cccccggacc
     6121 acggcctgtg agcgcgcgca gaaaggattt gcgcgaaaca gacagaagca ccggcaaatc
     6181 gaagcgcagc cgcaattcat cgaaccgcgc cagcaccgag agcgaggttt cgggagcagc
     6241 ccccagaaaa aaccccatgc cgggatcaag gacaaggcgg ttgcgtttga taccggcacc
     6301 cgtcagcgcc gcgatgcgcg cgtcaaagaa cgccgcaatg tgatccatga tgtcgccagc
     6361 gggtgcctcg cgccgatctg cctgcccgtc ttgcaccgaa tgcataacga cgagtttggc
     6421 agatgatttc gccaattgcg gatagaacgc agcgtctgga aaaccgcgaa tatcattgag
     6481 ataggccaca ccacgcgaca aggcataggc ttgcgtcgcg ggttgataac tgtcgagcga
     6541 gacgggaatg ccatctgcct tgagcgcgtc cagcaccggc gcgatacgcg cgatttctgt
     6601 gtcggacgaa acaggcgcgg cgtcgggatt gctggatgcc ggaccgaggt cgatcacatc
     6661 tgccccctcg gccatcagct tacgcgcctg cgcaatggct gcgtctggcg ccagataccg
     6721 gcctccatcg gagaaactgt ccgaggttat gttgacgatg ccgaaaatga tgagcgattt
     6781 attcatgggg gcttctataa taataataat cgagcatgag tctcatacgg atgctcgggt
     6841 cgaaagggaa tccccaggcg agtaacctgt ttgcggtgat ccattagctg caggagcaga
     6901 agagcataca tctggaagca aagccaggaa agcggcctat ggagctgtgc ggcagcgctc
     6961 agtaggcaat ttttcaaaat attgttaagc cttttctgag catggtattt ttcatggtat
     7021 taccaattag caggaaaata agccattgaa tataaaagat aaaaatgtct tgtttacaat
     7081 agagtggggg ggtcagcctg ccgccttggg ccgggtgatg tcgtacttgc ccgccgcgaa
     7141 ctcggttacc gtccagccca gcgcgaccag ctccggcaac gcctcgcgca cccgctggcg
     7201 gcgcttgcgc atggtcgaac cactggcctc tgacggccag acatagccgc acaaggtatc
     7261 tatggaagcc ttgccggttt tgccggggtc gatccagcca cacagccgct ggtgcagcag
     7321 gcgggcggtt tcgctgtcca gcgcccgcac ctcgtccatg ctgatgcgca catgctggcc
     7381 gccacccatg acggcctgcg cgatcaaggg gttcagggcc acgtacaggc gcccgtccgc
     7441 ctcgtcgctg gcgtactccg acagcagccg aaacccctgc cgcttgcggc cattctgggc
     7501 gatgatggat accttccaaa ggcgctcgat gcagtcctgt atgtgcttga gcgccccacc
     7561 actatcgacc tctgccccga tttcctttgc cagcgcccga tagctacctt tgaccaccat
     7621 ggcatcagcg gtgacggcct cccacttggg ttccaggaac agccggagct gccgtccgcc
     7681 ttcggtcttg ggttccgggc caagcactag gccattaggc ccagccatgg ccaccagccc
     7741 ttgcaggatg cgcagatcat cagcgcccag cggctccggg ccgctgaact cgatccgctt
     7801 gccgtcgccg tagtcatacg tcacgtccag cttgctgcgc ttgcgctcgc cccgcttgag
     7861 ggcacggaac aggccggggg ccagacagtg cgccgggtcg tgccggacgt ggctgaggct
     7921 gtgcttgttc ttaggcttca ccacggggca cccccttgct cttgcgctgc ctctccagca
     7981 cggcgggctt gagcaccccg ccgtcatgcc gcctgaacca ccgatcagcg aacggtgcgc
     8041 catagttggc cttgctcaca ccgaagcgga cgaagaaccg gcgctggtcg tcgtccacac
     8101 cccattcctc ggcctcggcg ctggtcatgc tcgacaggta ggactgccag cggatgttat
     8161 cgaccagtac cgagctgccc cggctggcct gctgctggtc gcctgcgccc atcatggccg
     8221 cgcccttgct ggcatggtgc aggaacacga tagagcaccc ggtatcggcg gcgatggcct
     8281 ccatgcgacc gatgacctgg gccatggggc cgctggcgtt ttcttcctcg atgtggaacc
     8341 ggcgcagcgt gtccagcacc atcaggcggc ggccctcggc ggcgcgcttg aggccgtcga
     8401 accactccgg ggccatgatg ttgggcaggc tgccgatcag cggctggatc agcaggccgt
     8461 cagccacggc ttgccgttcc tcggcgctga ggtgcgcccc aagggcgtgc aggcggtgat
     8521 gaatggcggt gggcgggtct tcggcgggca ggtagatcac cgggccggtg ggcagttcgc
     8581 ccacctccag cagatccggc ccgcctgcaa tctgtgcggc cagttgcagg gccagcatgg
     8641 atttaccggc accaccgggc gacaccagcg ccccgaccgt accggccacc atgttgggca
     8701 aaacgtagtc cagcggtggc ggcgctgctg cgaacgcctc cagaatattg ataggcttat
     8761 gggtagccat tgattgcctc ctttgcaggc agttggtggt taggcgctgg cggggtcact
     8821 acccccgccc tgcgccgctc tgagttcttc caggcactcg cgcagcgcct cgtattcgtc
     8881 gtcggtcagc cagaacttgc gctgacgcat ccctttggcc ttcatgcgct cggcatatcg
     8941 cgcttggcgt acagcgtcag ggctggccag caggtcgccg gtctgcttgt ccttttggtc
     9001 tttcatatca gtcaccgaga aacttgccgg ggccgaaagg cttgtcttcg cggaacaagg
     9061 acaaggtgca gccgtcaagg ttaaggctgg ccatatcagc gactgaaaag cggccagcct
     9121 cggccttgtt tgacgtataa ccaaagccac cgggcaacca atagcccttg tcacttttga
     9181 tcaggtagac cgaccctgaa gcgctttttt cgtattccat aaaaccccct tctgtgcgtg
     9241 agtactcata gtataacagg cgtgagtacc aacgcaagca ctacatgctg aaatctggcc
     9301 cgcccctgtc catgcctcgc tggcggggtg ccggtgcccg tgccagctcg gcccgcgcaa
     9361 gctggacgct gggcagaccc atgaccttgc tgacggtgcg ctcgatgtaa tccgcttcgt
     9421 ggccgggctt gcgctctgcc agcgctgggc tggcctcggc catggccttg ccgatttcct
     9481 cggcactgcg gccccggctg gccagcttct gcgcggcgat aaagtcgcac ttgctgaggt
     9541 catcaccgaa gcgcttgacc agcccggcca tctcgctgcg gtactcgtcc agcgccgtgc
     9601 gccggtggcg gctaagctgc cgctcgggca gttcgaggct ggccagcctg cgggccttct
     9661 cctgctgccg ctgggcctgc tcgatctgct ggccagcctg ctgcaccagc gccgggccag
     9721 cggtggcggt cttgcccttg gattcacgca gcagcaccca cggctgataa ccggcgcggg
     9781 tggtgtgctt gtccttgcgg ttggtgaagc ccgccaagcg gccatagtgg cggctgtcgg
     9841 cgctggccgg gtcggcgtcg tactcgctgg ccagcgtccg ggcaatctgc ccccgaagtt
     9901 caccgcctgc ggcgtcggcc accttgaccc atgcctgata gttcttcggg ctggtttcca
     9961 ctaccagggc aggctcccgg ccctcggctt tcatgtcatc caggtcaaac tcgctgaggt
    10021 cgtccaccag caccagacca tgccgctcct gctcggcggg cctgatatac acgtcattgc
    10081 cctgggcatt catccgcttg agccatggcg tgttctggag cacttcggcg gctgaccatt
    10141 cccggttcat catctggccg gtggtggcgt ccctgacgcc gatatcgaag cgctcacagc
    10201 ccatggcctt gagctgtcgg cctatggcct gcaaagtcct gtcgttcttc atcgggccac
    10261 caagcgcagc cagatcgagc cgtcctcggt tgtcagtggc gtcaggtcga gcaagagcaa
    10321 cgatgcgatc agcagcacca ccgtaggcat catggaagcc agcatcacgg ttagccatag
    10381 cttccagtgc cacccccgcg acgcgctccg ggcgctctgc gcggcgctgc tcacctcggc
    10441 ggctacctcc cgcaactctt tggccagctc cacccatgcc gcccctgtct ggcgctgggc
    10501 tttcagccac tccgccgcct gcgcctcgct ggcctgctgg gtctggctca tgacctgccg
    10561 ggcttcgtcg gccagtgtcg ccatgctctg ggccagcggt tcgatctgct ccgctaactc
    10621 gttgatgcct ctggatttct tcactctgtc gattgcgttc atggtctatt gcctcccggt
    10681 attcctgtaa gtcgatgatc tgggcgttgg cggtgtcgat gttcagggcc acgtctgccc
    10741 ggtcggtgcg gatgccccgg ccttccatct ccaccacgtt cggccccagg tgaacaccgg
    10801 gcaggcgctc gatgccctgc gcctcaagtg ttctgtggtc aatgcgggcg tcgtggccag
    10861 cccgctctaa tgcccggttg gcatggtcgg cccatgcctc gcgggtctgc
//
