LOCUS       NZ_CP051390            11037 bp    DNA     circular CON 18-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Muenchen strain CVM
            22536 plasmid p22536, complete sequence.
ACCESSION   NZ_CP051390
VERSION     NZ_CP051390.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14504656
            Assembly: GCF_016454465.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Muenchen
  ORGANISM  Salmonella enterica subsp. enterica serovar Muenchen
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Zhao,S., Li,C., Hsu,C.H., Tyson,G.H., Strain,E., Tate,H., Tran,T.T.,
            Abbott,J. and McDermott,P.F.
  TITLE     Comparative Genomic Analysis of 450 Strains of Salmonellaenterica
            Isolated from Diseased Animals
  JOURNAL   Genes (Basel) 11 (9), 1025 (2020)
   PUBMED   32883017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 609x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016454465.1-RS_2024_05_18
            Annotation Date                   :: 05/18/2024 02:19:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,605
            CDSs (total)                      :: 4,482
            Genes (coding)                    :: 4,367
            CDSs (with protein)               :: 4,367
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 53 of 115
            Pseudo Genes (incomplete)         :: 80 of 115
            Pseudo Genes (internal stop)      :: 19 of 115
            Pseudo Genes (multiple problems)  :: 32 of 115
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051390.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Salmonella enterica subsp. enterica serovar
                     Muenchen"
                     /mol_type="genomic DNA"
                     /strain="CVM 22536"
                     /serovar="Muenchen"
                     /isolation_source="swine"
                     /sub_species="enterica"
                     /db_xref="taxon:596"
                     /plasmid="p22536"
                     /geo_loc_name="USA:NC"
                     /collection_date="2002"
                     /collected_by="NC State Veterinary Diagnostic Lab"
     gene            1..>642
                     /locus_tag="HFR50_RS23135"
                     /pseudo=""
     CDS             1..>642
                     /locus_tag="HFR50_RS23135"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            749..1162
                     /locus_tag="HFR50_RS23140"
     CDS             749..1162
                     /locus_tag="HFR50_RS23140"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            1261..2130
                     /locus_tag="HFR50_RS23145"
     CDS             1261..2130
                     /locus_tag="HFR50_RS23145"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            2191..2403
                     /locus_tag="HFR50_RS22850"
                     /old_locus_tag="HFR50_22865"
     CDS             2191..2403
                     /locus_tag="HFR50_RS22850"
                     /old_locus_tag="HFR50_22865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            2405..2611
                     /gene="urfF"
                     /locus_tag="HFR50_RS22855"
                     /old_locus_tag="HFR50_22870"
     CDS             2405..2611
                     /gene="urfF"
                     /locus_tag="HFR50_RS22855"
                     /old_locus_tag="HFR50_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            2641..3480
                     /locus_tag="HFR50_RS22860"
                     /old_locus_tag="HFR50_22875"
     CDS             2641..3480
                     /locus_tag="HFR50_RS22860"
                     /old_locus_tag="HFR50_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            3467..4318
                     /gene="repC"
                     /locus_tag="HFR50_RS22865"
                     /old_locus_tag="HFR50_22880"
     CDS             3467..4318
                     /gene="repC"
                     /locus_tag="HFR50_RS22865"
                     /old_locus_tag="HFR50_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            4625..5440
                     /gene="sul2"
                     /locus_tag="HFR50_RS22870"
                     /old_locus_tag="HFR50_22890"
     ARG             4626..5437
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5886..6304
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             5502..6301
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6304..>6411
                     /locus_tag="HFR50_RS22880"
                     /old_locus_tag="HFR50_22900"
                     /pseudo=""
     CDS             6304..>6411
                     /locus_tag="HFR50_RS22880"
                     /old_locus_tag="HFR50_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family protein"
                     /name="aminoglycoside phosphotransferase family protein"
     gene            6431..7267
                     /locus_tag="HFR50_RS22885"
                     /old_locus_tag="HFR50_22905"
     CDS             6431..7267
                     /locus_tag="HFR50_RS22885"
                     /old_locus_tag="HFR50_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            7573..7815
                     /locus_tag="HFR50_RS22890"
                     /old_locus_tag="HFR50_22915"
     CDS             7573..7815
                     /locus_tag="HFR50_RS22890"
                     /old_locus_tag="HFR50_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(7847..8497)
                     /gene="tetR(A)"
                     /locus_tag="HFR50_RS22895"
                     /old_locus_tag="HFR50_22920"
     CDS             complement(7847..8497)
                     /gene="tetR(A)"
                     /locus_tag="HFR50_RS22895"
                     /old_locus_tag="HFR50_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            8603..9802
                     /gene="tet(A)"
                     /locus_tag="HFR50_RS22900"
                     /old_locus_tag="HFR50_22925"
     ARG             8604..9799
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<9769..9957)
                     /locus_tag="HFR50_RS22905"
                     /old_locus_tag="HFR50_22930"
                     /pseudo=""
     CDS             complement(<9769..9957)
                     /locus_tag="HFR50_RS22905"
                     /old_locus_tag="HFR50_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(10555..10839)
                     /locus_tag="HFR50_RS22910"
                     /old_locus_tag="HFR50_22935"
     CDS             complement(10555..10839)
                     /locus_tag="HFR50_RS22910"
                     /old_locus_tag="HFR50_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     ARG             6432..7264
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6525..7267
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.19"
                     /coverage="88.85"
     Typing          10794..10949
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          2659..3480
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2..2127
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg
       61 gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg
      121 cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct
      181 gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg
      241 gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc
      301 ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac
      361 tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg
      421 ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc
      481 aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca
      541 ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc
      601 gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc
      661 cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac
      721 ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag
      781 aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc
      841 cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc aggcggcgga
      901 gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg
      961 ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg
     1021 gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct
     1081 gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg
     1141 gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc
     1201 aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac cggccagatg
     1261 atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg
     1321 aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg
     1381 ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct
     1441 gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc
     1501 gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac
     1561 ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac
     1621 aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag
     1681 accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag
     1741 cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc
     1801 cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc
     1861 ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc
     1921 cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc
     1981 aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc
     2041 agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg
     2101 gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac
     2161 tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg
     2221 gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca
     2281 aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc
     2341 tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac
     2401 tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt
     2461 acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg
     2521 gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca
     2581 gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca
     2641 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
     2701 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
     2761 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
     2821 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
     2881 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
     2941 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
     3001 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
     3061 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
     3121 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
     3181 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
     3241 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
     3301 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
     3361 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
     3421 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
     3481 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
     3541 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
     3601 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     3661 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     3721 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     3781 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     3841 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     3901 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     3961 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     4021 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     4081 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     4141 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     4201 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     4261 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     4321 cccccactct attgtaaaca agacattttt atcttttata ttcaatggct tattttcctg
     4381 ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat attttgaaaa
     4441 attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt tgcttccaga
     4501 tgtatgctct tctgctcctg cagctaatgg atcaccgcaa acaggttact cgcctgggga
     4561 ttccctttcg acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc
     4621 ccccatgaat aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc
     4681 cgatggaggc cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc
     4741 cgagggggca gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt
     4801 ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg
     4861 cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg
     4921 tgtggcctat ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc
     4981 gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg
     5041 cgaggcaccc gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc
     5101 ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt
     5161 ttttctgggg gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg
     5221 gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct
     5281 cacaggccgt ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc
     5341 cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc
     5401 ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc
     5461 tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt
     5521 ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt
     5581 ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga
     5641 gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga
     5701 ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg
     5761 agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca
     5821 gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc gcaggctgtc
     5881 gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt
     5941 accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct
     6001 accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat
     6061 gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg
     6121 gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa
     6181 ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat
     6241 cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg
     6301 ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt
     6361 gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac gaccctctga
     6421 cttggggttg atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt
     6481 tctcattgcg gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc
     6541 tgcaatcgtc aagggattga aacctataga agacattgct gatgaactgc gcggggccga
     6601 ctatctggta tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct
     6661 gatgttgctc gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga
     6721 ctaccaggcg accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga
     6781 acccctgcct tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc
     6841 gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga
     6901 tcaaatgatg agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa
     6961 catcatgttc tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt
     7021 gggctttggc gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga
     7081 tcctagacgc attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg
     7141 tcgcctgctc gaccaggcgt acgcttatgg atgcctttcc gcagcttgga acgcggatgg
     7201 agaagaggag caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc
     7261 atactagata tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat
     7321 atcgctggcc aaagccttag cgtaggattc cgccccttcc cgcaaacgac ccctggaagc
     7381 cccgtagcga cgcggagagg ggcgagacaa gccaagggcg caggctcgat gcgcagcacg
     7441 acatagccgg ttctcgcaag gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa
     7501 agtttccaac gcgagccatt cgcgagagcc ttgagtccac gctagatcta tctcatctgc
     7561 gcaaggcaga acgtgaagac ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag
     7621 gccggcgcgc ggacccgcgc cacggcccac gagcggacgc cgcagcagga gcgccagaag
     7681 gccgccagag aggccgagcg cggccgtgag gcttggacgc tagggcaggg catgaaaaag
     7741 cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag ggggagggga tgttgtctac
     7801 atggctctgc tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa tcgtcaccct
     7861 ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt cgccaatcca tcgacaatca
     7921 ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg
     7981 cccgcaacag cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc
     8041 tgtcgccggc ctgctcctca agcacggccc caacagtgaa gtagctgatt gtcatcagcg
     8101 cattgacggc gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg
     8161 ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt
     8221 aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag cgccagtcgt
     8281 cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg gccagtgcgt
     8341 cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga acccccaacc
     8401 gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg
     8461 cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta tcactgataa
     8521 acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat cggaccagcg
     8581 gaggctggtc cggaggccag acgtgaaacc caacagaccc ctgatcgtaa ttctgagcac
     8641 tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg gcctcctgcg
     8701 cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg cgctgtatgc
     8761 gttgatgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg
     8821 gccggtcttg ctcgtctcgc tggccggcgc tgctgtcgac tacgccatca tggcgacggc
     8881 gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg
     8941 ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg cgcggcactt
     9001 cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct
     9061 gatgggcggt ttctcccccc acgctccgtt cttcgccgcg gcagccttga acggcctcaa
     9121 tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg
     9181 ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc
     9241 cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg ccgcgctttg
     9301 ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca tttcgcttgc
     9361 cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg tagccgcccg
     9421 gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag gctacatcct
     9481 gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc ttgcttcggg
     9541 tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggatg aggaacgtca
     9601 ggggcagctg caaggctcac tggcggcgct caccagcctg acctcgatcg tcggacccct
     9661 cctcttcacg gcgatctatg cggcttctat aacaacgtgg aacgggtggg catggattgc
     9721 aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt ggagaaattc
     9781 ttcaaattcc cgttgcacat agcccggcaa ttcctttccc tgctctgcca taagcgcagc
     9841 gaatgccggg taatactcgt caacgatctg atagagaagg gtttgctcgg gtcggtggct
     9901 ctggtaacga ccagtatccc gatcccggct ggccgtcctg gccgccacat gaggcatgtt
     9961 ccgcgtcctt gcaatactgt gtttacatac agtctatcgc ttagcggaaa gttcttttac
    10021 cctcagccga aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccgtac
    10081 taactgtcac gcccccctgc aataactgtc acgcccccct gcaataactg tcacgccccc
    10141 ctgcaataac tgtcacgccc ccctgcaata actgtcacga acccctgcaa taactgtcac
    10201 gcccccaaac ctgcaaaccc agcaggggcg ggggctggcg gggtgttgga aaaatccatc
    10261 catgattatc taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct
    10321 gcccttgccc aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct
    10381 acacaccgcc ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac
    10441 accaaacccc gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta
    10501 cccgaagggt gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc
    10561 cggctcatcc ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag
    10621 gtacgcatcc attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc
    10681 caaaatcatg gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg
    10741 ttcccttgcc cgcacccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt
    10801 ggccagccga tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg
    10861 tgctgtctcg taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg
    10921 ggagggcgca cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg
    10981 tgcgctctcc gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagc
//
