LOCUS       NZ_CP051365             8688 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Choleraesuis strain CVM
            28296 plasmid p28296-2, complete sequence.
ACCESSION   NZ_CP051365
VERSION     NZ_CP051365.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14504742
            Assembly: GCF_016454285.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Choleraesuis
  ORGANISM  Salmonella enterica subsp. enterica serovar Choleraesuis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Zhao,S., Li,C., Hsu,C.H., Tyson,G.H., Strain,E., Tate,H., Tran,T.T.,
            Abbott,J. and McDermott,P.F.
  TITLE     Comparative Genomic Analysis of 450 Strains of Salmonellaenterica
            Isolated from Diseased Animals
  JOURNAL   Genes (Basel) 11 (9), 1025 (2020)
   PUBMED   32883017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 972x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016454285.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:49:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,602
            Genes (coding)                    :: 4,319
            CDSs (with protein)               :: 4,319
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 283
            CDSs (without protein)            :: 283
            Pseudo Genes (ambiguous residues) :: 0 of 283
            Pseudo Genes (frameshifted)       :: 132 of 283
            Pseudo Genes (incomplete)         :: 122 of 283
            Pseudo Genes (internal stop)      :: 86 of 283
            Pseudo Genes (multiple problems)  :: 50 of 283
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051365.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Salmonella enterica subsp. enterica serovar
                     Choleraesuis"
                     /mol_type="genomic DNA"
                     /strain="CVM 28296"
                     /serovar="Choleraesuis"
                     /isolation_source="swine"
                     /sub_species="enterica"
                     /db_xref="taxon:119912"
                     /plasmid="p28296-2"
                     /geo_loc_name="USA:MO"
                     /collection_date="2002"
                     /collected_by="MO State Veterinary Diagnostic Lab"
     gene            join(8636..8688,1..604)
                     /locus_tag="HFS36_RS23430"
     CDS             join(8636..8688,1..604)
                     /locus_tag="HFS36_RS23430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_244664296.1"
                     /translation="MVGPCLAGLLKPCLGLERFGLLCPALLRGLAVVPLEPLSGGPLDA
                     VIDPLGDHQVAVRVLAATGMDGQRIRQALGTGQVLGELGRQRLLLVEGQLDRQGKFDLL
                     EQPPIGAFIQVGSIPVVGGPLDELRHVPGFGVQDFIHVAGILAFALDVVGLGPGRLAAR
                     PAAGFRRKVINRHAASLLLLLFGSMQWPSESAPPEGWPLGQFLEEKPVSAPSPTK"
                     /name="hypothetical protein"
     gene            674..958
                     /locus_tag="HFS36_RS00010"
                     /old_locus_tag="HFS36_00010"
     CDS             674..958
                     /locus_tag="HFS36_RS00010"
                     /old_locus_tag="HFS36_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            1512..>1916
                     /locus_tag="HFS36_RS00015"
                     /old_locus_tag="HFS36_00015"
                     /pseudo=""
     CDS             1512..>1916
                     /locus_tag="HFS36_RS00015"
                     /old_locus_tag="HFS36_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2023..2859)
                     /locus_tag="HFS36_RS00020"
                     /old_locus_tag="HFS36_00020"
     CDS             complement(2023..2859)
                     /locus_tag="HFS36_RS00020"
                     /old_locus_tag="HFS36_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480972.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(2860..3278)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             complement(2863..3662)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3723..4538)
                     /gene="sul2"
                     /locus_tag="HFS36_RS00030"
                     /old_locus_tag="HFS36_00030"
     ARG             complement(3727..4538)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4846..5697)
                     /gene="repC"
                     /locus_tag="HFS36_RS00035"
                     /old_locus_tag="HFS36_00040"
     CDS             complement(4846..5697)
                     /gene="repC"
                     /locus_tag="HFS36_RS00035"
                     /old_locus_tag="HFS36_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(5684..6523)
                     /locus_tag="HFS36_RS00040"
                     /old_locus_tag="HFS36_00045"
     CDS             complement(5684..6523)
                     /locus_tag="HFS36_RS00040"
                     /old_locus_tag="HFS36_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(6553..6759)
                     /gene="urfF"
                     /locus_tag="HFS36_RS00045"
                     /old_locus_tag="HFS36_00050"
     CDS             complement(6553..6759)
                     /gene="urfF"
                     /locus_tag="HFS36_RS00045"
                     /old_locus_tag="HFS36_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(6761..6973)
                     /locus_tag="HFS36_RS00050"
                     /old_locus_tag="HFS36_00055"
     CDS             complement(6761..6973)
                     /locus_tag="HFS36_RS00050"
                     /old_locus_tag="HFS36_00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(7034..8005)
                     /locus_tag="HFS36_RS23435"
     CDS             complement(7034..8005)
                     /locus_tag="HFS36_RS23435"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_010890158.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(8002..8415)
                     /locus_tag="HFS36_RS23440"
     CDS             complement(8002..8415)
                     /locus_tag="HFS36_RS23440"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     ARG             complement(2027..2859)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(565..720)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(5685..6506)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(7038..8686)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cttcggtctt ctgtgccccg cccttctccg gggtcttgcc gttgtaccgc ttgaaccact
       61 gagcggcggg ccgctcgatg ccgtcattga tccgctcgga gatcatcagg tggcagtgcg
      121 ggttctcgcc gccaccggca tggatggcca gcgtatacgg caggcgctcg gcaccggtca
      181 ggtgctgggc gaactcggac gccagcgcct tctgctggtc gagggtcagc tcgaccggca
      241 gggcaaattc gacctccttg aacagccgcc cattggcgcg ttcatacagg tcggcagcat
      301 cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg cccggattcg gcgtgcaaga
      361 cttcatccat gtcgcgggca tacttgcctt cgcgctggat gtagtcggcc ttggccctgg
      421 ccgattggcc gcccgacctg ctgccggttt tcgccgtaag gtgataaatc gccatgctgc
      481 ctcgctgttg cttttgcttt tcggctccat gcaatggccc tcggagagcg caccgcccga
      541 agggtggccg ttaggccagt ttctcgaaga gaaaccggta agtgcgccct cccctacaaa
      601 gtagggtcgg gattgccgcc gctgtgcctc catgatagcc tacgagacag cacattaaca
      661 atggggtgtc aagatggtta aggggagcaa caaggcggcg gatcggctgg ccaagctcga
      721 agaacaacga gcgcgaatca atgccgaaat tcagcgggta cgggcaaggg aacagcagca
      781 agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg gccatgattt tggccaaggt
      841 gaacagcagc gagtggccgg aggatcggct catggcggca atggatgcgt accttgaacg
      901 cgaccacgac cgcgccttgt tcggtctgcc gccacgccag aaggatgagc cgggctgaat
      961 gatcgaccga gacaggccct gcggggctgc acacgcgccc ccacccttcg ggtaggggga
     1021 aaggccgcta aagcggctaa aagcgctcca gcgtatttct gcggggtttg gtgtggggtt
     1081 tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt ggggcggtgt gtagcctagc
     1141 gcagcgaata gaccagctat ccggcctctg gccgggcata ttgggcaagg gcagcagcgc
     1201 cccacaaggg cgctgataac cgcgcctagt ggattattct tagataatca tggatggatt
     1261 tttccaacac cccgccagcc cccgcccctg ctgggtttgc aggtttgggg gcgtgacagt
     1321 tattgcaggg gttcgtgaca gttattgcag gggggcgtga cagttattgc aggggttcgt
     1381 gacagttagt acgggagtga cgggcactgg ctggcaatgt ctagcaacgg caggcatttc
     1441 ggctgagggt aaaagaactt tccgctaagc gatagactgt atgtaaacac agtattgcaa
     1501 ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg gatactggtc
     1561 gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac gagtattacc
     1621 cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat gtgcaacggg
     1681 aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttctacgg gttcgctgcg
     1741 agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa gcgtcgcggt ttctgcccga
     1801 gctgtggggc gcggcggatg gccgaaagtg ccgccttgct ggttgatgaa gtactgcctg
     1861 aacaacccat gcgtcagtgg gtgttgagct tcccgtttca gctgcgtttc ctgtttgggg
     1921 tcgtttgcgg gaaggggcgg aatcctacgc taaggctttg gccagcgata ttctccggtg
     1981 agattgatgt gttcccaggg gataggagaa gtcgcttgat atctagtatg acgtctgtcg
     2041 cacctgcttg atcgcggccg cgatagctag atcgcgttgc tcctcttctc catccgcgtt
     2101 ccaagctgcg gaaaggcacc cataagcgta cgcctggtcg agcaggcgac gcggatcgac
     2161 gtccagcgca cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat cgagacaaag
     2221 gtcgtctctg tcagccggat cgtagaacat attggcggcg ccaaagccca cttcaccgac
     2281 cagaccgacg ggatctatca ccagccagcc gcgactggag aacatgatgt tttcatgatg
     2341 cagatcgcca tgtagcccac gcagttccga ggcattgctc atcatttgat cggctataat
     2401 cgccgcgtgg acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg cccgctgaaa
     2461 caaagctgca aagcgatccc ggatcgggag aagggcagaa ggcaggggtt cctcagatgc
     2521 ggcatacagc ttcgccatta gttccgctgc aatttcggtc gcctggtagt cgccgtgctc
     2581 ggcaacgatg tgagagagca ttcgctcccc ggcatattcg agcaacatca gattgttctc
     2641 acgaccgagc aaccggactg ctcccctccc attgcgccat accagatagt cggccccgcg
     2701 cagttcatca gcaatgtctt ttataggttt caatcccttg acgattgcag gagtcccgtc
     2761 tggcaatgaa actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa caggttgcga
     2821 aacgtgccaa tgagcaggaa aaacaggcgg catgaacatc aaccccaagt cagagggtcc
     2881 aatcgcagat agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa tacattgaat
     2941 aggacagcga aggcgcgctc tgcttcatct ggcgctgccc agttctcttc ggcgttagca
     3001 atcatgagtg ccaaatcggc atagcgatct gctgttccga gccgcccaag gtcgatcaga
     3061 cccgtgcatt gaagagtttt agggtccacc atgaagttcg gcatgcaggg atcaccatgg
     3121 caaacaacca tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg ttcgacacga
     3181 gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc gggattgacg
     3241 gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct gcgctcaaac
     3301 ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat tgacggccac
     3361 gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat tgccgttatc
     3421 accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca agccacacct
     3481 cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg gcgggaagca
     3541 ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc accagattct
     3601 ccgcctctga caggcaacca gtcagaatgc gattcaccaa aaaaaatatt agttcgattc
     3661 aatggaggtt ccttcagttt tctgatgaag cgcgaatata gagaaatatc ccgaatgtgc
     3721 agttaacgaa ttcttgcggt ttctttcagc gccgccaata ccgccagccc gtcgcgcaag
     3781 gggcgcggct cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc aagctctgca
     3841 gcgagtgtcg cggccccgac atcccccgga ccacggcctg tgagcgcgcg cagaaaggat
     3901 ttgcgcgaaa cagacagaag caccggcaaa tcgaagcgca gccgcaattc atcgaaccgc
     3961 gccagcaccg agagcgaggt ttcgggagca gcccccagaa aaaaccccat gccgggatca
     4021 aggacaaggc ggttgcgttt gataccggca cccgtcagcg ccgcgatgcg cgcgtcaaag
     4081 aacgccgcaa tgtgatccat gatgtcgcca gcgggtgcct cgcgccgatc tgcctgcccg
     4141 tcttgcaccg aatgcataac gacgagtttg gcagatgatt tcgccaattg cggatagaac
     4201 gcagcgtctg gaaaaccgcg aatatcattg agataggcca caccacgcga caaggcatag
     4261 gcttgcgtcg cgggttgata actgtcgagc gagacgggaa tgccatctgc cttgagcgcg
     4321 tccagcaccg gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc ggcgtcggga
     4381 ttgctggatg ccggaccgag gtcgatcaca tctgccccct cggccatcag cttacgcgcc
     4441 tgcgcaatgg ctgcgtctgg cgccagatac cggcctccat cggagaaact gtccgaggtt
     4501 atgttgacga tgccgaaaat gatgagcgat ttattcatgg gggcttctat aataataata
     4561 atcgagcatg agtatcatac ggatgctcgg gtcgaaaggg aatccccagg cgagtaacct
     4621 gtttgcggtg atccattagc tgcaggagca gaagagcata catctggaag caaagccagg
     4681 aaagcggcct atggagctgt gcggcagcgc tcagtaggca atttttcaaa atattgttaa
     4741 gccttttctg agcatggtat ttttcatggt attaccaatt agcaggaaaa taagccattg
     4801 aatataaaag ataaaaatgt cttgtttaca atagagtggg gggggtcagc ctgccgcctt
     4861 gggccgggtg atgtcgtact tgcccgccgc gaactcggtt accgtccagc ccagcgcgac
     4921 cagctccggc aacgcctcgc gcacccgctg gcggcgcttg cgcatggtcg aaccactggc
     4981 ctctgacggc cagacatagc cgcacaaggt atctatggaa gccttgccgg ttttgccggg
     5041 gtcgatccag ccacacagcc gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg
     5101 cacctcgtcc atgctgatgc gcacatgctg gccgccaccc atgacggcct gcgcgatcaa
     5161 ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag
     5221 ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg gataccttcc aaaggcgctc
     5281 gatgcagtcc tgtatgtgct tgagcgcccc accactatcg acctctgccc cgatttcctt
     5341 tgccagcgcc cgatagctac ctttgaccac catggcatca gcggtgacgg cctcccactt
     5401 gggttccagg aacagccgga gctgccgtcc gccttcggtc ttgggttccg ggccaagcac
     5461 taggccatta ggcccagcca tggccaccag cccttgcagg atgcgcagat catcagcgcc
     5521 cagcggctcc gggccgctga actcgatccg cttgccgtcg ccgtagtcat acgtcacgtc
     5581 cagcttgctg cgcttgcgct cgccccgctt gagggcacgg aacaggccgg gggccagaca
     5641 gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg ttcttaggct tcaccacggg
     5701 gcaccccctt gctcttgcgc tgcctctcca gcacggcggg cttgagcacc ccgccgtcat
     5761 gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt ggccttgctc acaccgaagc
     5821 ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc ctcggcctcg gcgctggtca
     5881 tgctcgacag gtaggactgc cagcggatgt tatcgaccag taccgagctg ccccggctgg
     5941 cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt gctggcatgg tgcaggaaca
     6001 cgatagagca cccggtatcg gcggcgatgg cctccatgcg accgatgacc tgggccatgg
     6061 ggccgctggc gttttcttcc tcgatgtgga accggcgcag cgtgtccagc accatcaggc
     6121 ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc cggggccatg atgttgggca
     6181 ggctgccgat cagcggctgg atcagcaggc cgtcagccac ggcttgccgt tcctcggcgc
     6241 tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc ggtgggcggg tcttcggcgg
     6301 gcaggtagat caccgggccg gtgggcagtt cgcccacctc cagcagatcc ggcccgcctg
     6361 caatctgtgc ggccagttgc agggccagca tggatttacc ggcaccaccg ggcgacacca
     6421 gcgccccgac cgtaccggcc accatgttgg gcaaaacgta gtccagcggt ggcggcgctg
     6481 ctgcgaacgc ctccagaata ttgataggct tatgggtagc cattgattgc ctcctttgca
     6541 ggcagttggt ggttaggcgc tggcggggtc actacccccg ccctgcgccg ctctgagttc
     6601 ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc agccagaact tgcgctgacg
     6661 catccctttg gccttcatgc gctcggcata tcgcgcttgg cgtacagcgt cagggctggc
     6721 cagcaggtcg ccggtctgct tgtccttttg gtctttcata tcagtcaccg agaaacttgc
     6781 cggggccgaa aggcttgtct tcgcggaaca aggacaaggt gcagccgtca aggttaaggc
     6841 tggccatatc agcgactgaa aagcggccag cctcggcctt gtttgacgta taaccaaagc
     6901 caccgggcaa ccaatagccc ttgtcacttt tgatcaggta gaccgaccct gaagcgcttt
     6961 tttcgtattc cataaaaccc ccttctgtgc gtgagtactc atagtataac aggcgtgagt
     7021 accaacgcaa gcactacatg ctgaaatctg gcccgcccct gtccatgcct cgctggcggg
     7081 gtgccggtgc ccgtgccagc tcggcccgcg caagctggac gctgggcaga cccatgacct
     7141 tgctgacggt gcgctcgatg taatccgctt cgtggccggg cttgcgctct gccagcgctg
     7201 ggctggcctc ggccatggcc ttgccgattt cctcggcact gcggccccgg ctggccagct
     7261 tctgcgcggc gataaagtcg cacttgctga ggtcatcacc gaagcgcttg accagcccgg
     7321 ccatctcgct gcggtactcg tccagcgccg tgcgccggtg gcggctaagc tgccgctcgg
     7381 gcagttcgag gctggccagc ctgcgggcct tctcctgctg ccgctgggcc tgctcgatct
     7441 gctggccagc ctgctgcacc agcgccgggc cagcggtggc ggtcttgccc ttggattcac
     7501 gcagcagcac ccacggctga taaccggcgc gggtggtgtg cttgtccttg cggttggtga
     7561 agcccgccaa gcggccatag tggcggctgt cggcgctggc cgggtcggcg tcgtactcgc
     7621 tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc tgcggcgtcg gccaccttga
     7681 cccatgcctg atagttcttc gggctggttt ccactaccag ggcaggctcc cggccctcgg
     7741 ctttcatgtc atccaggtca aactcgctga ggtcgtccac cagcaccaga ccatgccgct
     7801 cctgctcggc gggcctgata tacacgtcat tgccctgggc attcatccgc ttgagccatg
     7861 gcgtgttctg gagcacttcg gcggctgacc attcccggtt catcatctgg ccggtggtgg
     7921 cgtccctgac gccgatatcg aagcgctcac agcccatggc cttgagctgt cggcctatgg
     7981 cctgcaaagt cctgtcgttc ttcatcgggc caccaagcgc agccagatcg agccgtcctc
     8041 ggttgtcagt ggcgtcaggt cgagcaagag caacgatgcg atcagcagca ccaccgtagg
     8101 catcatggaa gccagcatca cggttagcca tagcttccag tgccaccccc gcgacgcgct
     8161 ccgggcgctc tgcgcggcgc tgctcacctc ggcggctacc tcccgcaact ctttggccag
     8221 ctccacccat gccgcccctg tctggcgctg ggctttcagc cactccgccg cctgcgcctc
     8281 gctggcctgc tgggtctggc tcatgacctg ccgggcttcg tcggccagtg tcgccatgct
     8341 ctgggccagc ggttcgatct gctccgctaa ctcgttgatg cctctggatt tcttcactct
     8401 gtcgattgcg ttcatggtct attgcctccc ggtattcctg taagtcgatg atctgggcgt
     8461 tggcggtgtc gatgttcagg gccacgtctg cccggtcggt gcggatgccc cggccttcca
     8521 tctccaccac gttcggcccc aggtgaacac cgggcaggcg ctcgatgccc tgcgcctcaa
     8581 gtgttctgtg gtcaatgcgg gcgtcgtggc cagcccgctc taatgcccgg ttggcatggt
     8641 cggcccatgc ctcgcgggtc tgctcaagcc atgccttggg cttgagcg
//
