LOCUS       NZ_CP051355            10454 bp    DNA     circular CON 15-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Worthington strain CVM
            30148 plasmid p30148-2, complete sequence.
ACCESSION   NZ_CP051355
VERSION     NZ_CP051355.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14504796
            Assembly: GCF_016454225.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Worthington
  ORGANISM  Salmonella enterica subsp. enterica serovar Worthington
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Zhao,S., Li,C., Hsu,C.H., Tyson,G.H., Strain,E., Tate,H., Tran,T.T.,
            Abbott,J. and McDermott,P.F.
  TITLE     Comparative Genomic Analysis of 450 Strains of Salmonellaenterica
            Isolated from Diseased Animals
  JOURNAL   Genes (Basel) 11 (9), 1025 (2020)
   PUBMED   32883017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 675x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016454225.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:59:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,884
            Genes (coding)                    :: 4,744
            CDSs (with protein)               :: 4,744
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 92 of 140
            Pseudo Genes (internal stop)      :: 33 of 140
            Pseudo Genes (multiple problems)  :: 35 of 140
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Salmonella enterica subsp. enterica serovar
                     Worthington"
                     /mol_type="genomic DNA"
                     /strain="CVM 30148"
                     /serovar="Worthington"
                     /isolation_source="swine"
                     /sub_species="enterica"
                     /db_xref="taxon:1160769"
                     /plasmid="p30148-2"
                     /geo_loc_name="USA:NC"
                     /collection_date="2003"
                     /collected_by="NC State Veterinary Diagnostic Lab"
     gene            join(10179..10454,1..681)
                     /locus_tag="HFS90_RS24770"
                     /old_locus_tag="HFS90_24775"
     CDS             join(10179..10454,1..681)
                     /locus_tag="HFS90_RS24770"
                     /old_locus_tag="HFS90_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_039899550.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="replication initiation protein"
     gene            689..1138
                     /locus_tag="HFS90_RS24775"
                     /old_locus_tag="HFS90_24780"
     CDS             689..1138
                     /locus_tag="HFS90_RS24775"
                     /old_locus_tag="HFS90_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_065314673.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPATQEPATQPVEKKSWRFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            1445..2413
                     /locus_tag="HFS90_RS24780"
                     /old_locus_tag="HFS90_24785"
     CDS             1445..2413
                     /locus_tag="HFS90_RS24780"
                     /old_locus_tag="HFS90_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_012904651.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(2874..3131)
                     /locus_tag="HFS90_RS24785"
                     /old_locus_tag="HFS90_24790"
     CDS             complement(2874..3131)
                     /locus_tag="HFS90_RS24785"
                     /old_locus_tag="HFS90_24790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001312881.1"
                     /translation="MAQIGTSQSVRREGISRRSDTIRPDASQLDLLFDDDAGRQRRERA
                     SAAIDGLIALSGRTVISLSERKPPAGGHVGGKIPYTRIPS"
                     /name="hypothetical protein"
     gene            complement(3579..4118)
                     /locus_tag="HFS90_RS24790"
                     /old_locus_tag="HFS90_24795"
                     /pseudo=""
     CDS             complement(3579..4118)
                     /locus_tag="HFS90_RS24790"
                     /old_locus_tag="HFS90_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710160.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4105..4764)
                     /gene="tetR(C)"
                     /locus_tag="HFS90_RS24795"
                     /old_locus_tag="HFS90_24800"
     CDS             complement(4105..4764)
                     /gene="tetR(C)"
                     /locus_tag="HFS90_RS24795"
                     /old_locus_tag="HFS90_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(C)"
                     /protein_id="WP_001038045.1"
                     /translation="MNKLQREAVIRTALELLNDVGMEGLTTRRLAERLGVQQPALYWHF
                     KNKRALLDALAEAMLTINHTHSTPRDDDDWRSFLKGNACSFRRALLAYRDGARIHAGTR
                     PAAPQMEKADAQLRFLCDAGFSAGDATYALMAISYFTVGAVLEQQASEADAEERGEDQL
                     TTSASTMPARLQSAMKIVYEGGPDAAFERGLALIIGGLEKMRLTTNDIEVLKNVDE"
                     /name="tetR(C)"
     gene            4983..6176
                     /gene="tet(C)"
                     /locus_tag="HFS90_RS24800"
                     /old_locus_tag="HFS90_24815"
                     /pseudo=""
     ARG             5416..6176
                     /name="tet(C)"
                     /gene="tet(C)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="63.89"
     gene            6080..6418
                     /locus_tag="HFS90_RS24805"
                     /old_locus_tag="HFS90_24820"
     CDS             6080..6418
                     /locus_tag="HFS90_RS24805"
                     /old_locus_tag="HFS90_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005291943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002310911.1"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFTT
                     PRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRISGSVGSWPRVRMIVLLSLRTRL
                     GWRGCLTG"
                     /name="hypothetical protein"
     gene            complement(6415..6627)
                     /locus_tag="HFS90_RS24810"
                     /old_locus_tag="HFS90_24825"
     CDS             complement(6415..6627)
                     /locus_tag="HFS90_RS24810"
                     /old_locus_tag="HFS90_24825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001326882.1"
                     /translation="MALTLSHSIVVSPVGSTLCCGQLQQHVGDFRVSRLYETRKPKTIH
                     VVAQVADVLQQQSLHVRSRIGDSFC"
                     /name="hypothetical protein"
     gene            6626..>6922
                     /locus_tag="HFS90_RS24815"
                     /old_locus_tag="HFS90_24830"
                     /pseudo=""
     CDS             6626..>6922
                     /locus_tag="HFS90_RS24815"
                     /old_locus_tag="HFS90_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(7239..7823)
                     /locus_tag="HFS90_RS24820"
                     /old_locus_tag="HFS90_24835"
     CDS             complement(7239..7823)
                     /locus_tag="HFS90_RS24820"
                     /old_locus_tag="HFS90_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(7836..8951)
                     /gene="mobA"
                     /locus_tag="HFS90_RS24825"
                     /old_locus_tag="HFS90_24840"
     CDS             complement(7836..8951)
                     /gene="mobA"
                     /locus_tag="HFS90_RS24825"
                     /old_locus_tag="HFS90_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            9115..9582
                     /gene="traD"
                     /locus_tag="HFS90_RS24830"
                     /old_locus_tag="HFS90_24845"
     CDS             9115..9582
                     /gene="traD"
                     /locus_tag="HFS90_RS24830"
                     /old_locus_tag="HFS90_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_058712813.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLIMAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     Typing          2..675
                     /name="rep_cluster_1787"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.483"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7840..8951)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.07"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcgtgttt ttaattgggc tgaatatgca gagttcaata gggaaacaca agaggtaact
       61 ttagttttta gtaaatatat aatcccatta ctatttcacc taaagagatt cattaaatac
      121 aacctcgagc atgttaagtc ctttgaaaat aaatactcaa tgcgcattta tgaatggctg
      181 ctaaaagaac taacgcaaag aaaaacacat aaagccaata tttcaataag cattgatgag
      241 tttaaattca tgctaatgtt agaaaaaagc tacccagaat ataaattact aaatcattgg
      301 gttttaaaac caatttcaaa agatttaaat acctatagca atataaaact atctattgat
      361 aagcgcggtc gtcctgctga tacgctgatc ttccaagttg aactggataa acaaattgac
      421 cttgtgactg agctagcaaa agaaccaata tcaaaaaaag aagataactc aatccgttta
      481 acgcctgaaa atcgtctgca tgaggggcta aaaacaacat tacatgatgc tttaactgcg
      541 aaaatacaac ttactggatt tgaagctaaa ttcctgagtg acatgcagag taagtatgat
      601 ctcaacggct catttacatg gctgactcaa aaacaacgag ccacgctaga gaaaatttta
      661 gctaagtacg gacggaactg aggtatctat ggctttagta tcaataagcg aagcagccag
      721 actaactgga aaaagccgca caacagtaca gtcatacatt aagcagggaa aactgaccaa
      781 gtgcacagat agcggtggtg cttcaaaact agacacatca gagcttctac gtgttttcgg
      841 atcatttact ggtcagcagg ttggcagtga acaatcaggc agtactgtgc agcatttggc
      901 aggcgatttt gtgcagttac ctgaccagaa tttgctacat gaaaactatg ccttaaaagc
      961 tgaaatagaa ctcgtaaagg cattgctgcg tgagaaagag ctactactcg atgaaaaaga
     1021 caaacgcaat gaggatctca aacatgcttt gctgctaatc gagtctaaac tgccagcaac
     1081 acaggaacct gccacgcagc cggtggaaaa aaaatcatgg cgattctgga agaaatagcg
     1141 gtttcagcca gtaaacaggt tgaaaccaat cccgcgatcc tgataacaaa ccggcaacat
     1201 aaaagcagcc cgttttcggg gtacaaaacc cgcgtttttg gacattccgg cgggttttga
     1261 aggtgagtgg tatggcgatg gtgcgcgcaa gatccattat gttaaacggg cgagtttaca
     1321 tctcaaaacc gcccgcttaa caccatcaga aatcctcagc gcgattttaa gcaccaaccc
     1381 ccccccgtaa cacccaaatc catactgaaa gtggctttgt tgaataaatc gaacttttgc
     1441 tgagttgaag gatcagatca cgcatcctcc cgacaacaca gaccattccg tggcaaagca
     1501 aaagttcaga atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct
     1561 cactttctgg ctggatgatg aggcgattca ggcctggtat gagtcggcaa caccttcatc
     1621 acgaggaagg ccccagcgct attctgatct cgccatcacc accgttctgg tgattaaacg
     1681 cgtattccgg ctgaccctgc gggctgcgca gggttttatt gattccattt ttgccctgat
     1741 gaacgttccg ttgcgctgcc cggattacac cagtgtcagt aagcgggcaa agtcggttaa
     1801 tgtcagtttc aaaacgtcca cccggggtga aatcgcacac ctggtgattg attccaccgg
     1861 gctgaaggtc tttggtgaag gcgaatggaa agtcagaaag cacggcaaag agcgccgtcg
     1921 tatctggcga aagttgcatc ttgctgttga cagcaacaca catgaagttg tctgtgcaga
     1981 cctgtcgctg aataacgtca cggactcaga agccttcccg ggccttatcc ggcagactca
     2041 cagaaaaatc agggcagccg cggcagacgg ggcttacgat acccggctct gtcacgatga
     2101 actgcgccgc aaaaaaatca gcgcgcttat tcctccccga aaaggtgcgg gttactggcc
     2161 cggtgaatat gcagaccgta accgtgcagt ggctaatcag cgaatgaccg ggagtaatgc
     2221 gcggtggaaa tggacaacag attacaaccg tcgctcgata gcggaaacgg cgatgtaccg
     2281 ggtaaaacag ctgttcgggg gttcactgac gctgcgtgac tacgatggtc aggttgcgga
     2341 ggctatggcc ctggtacgag cgctgaacaa aatgacgaaa gcaggtatgc ctgaaagcgt
     2401 gcgtattgcc tgaaaacaca acccgctacg ggggagactt acccgaaatc tgatttattc
     2461 aacaaagccg gttacccgcc ttatcactgt tgaaggccac cgctcatcac gcgtttgctg
     2521 acattgagcc ctgccggccg atcggcctga gactctcagc acaggagacg gcgggtgacg
     2581 agcgtatact ttttcgacgc ttggttgcgg tcgcgctagg cgtggccgcc gacgaacccg
     2641 ctggtgtcgc ccttgcggag cagcgcaagg cggaccttgc cacgacatcc cctcgcccgc
     2701 cgatcgtcta aattcgtcga ggttaagaaa ttcccgctcc ggcgaggcca tcaccatcac
     2761 cggcgtcagg tagtggacat ggcagcagcg cacgcaggtc atttccagtt tctggccgtc
     2821 gtcgagatcg ccgaccttca ggctccgctt ccagctcatc acccgaaatc ctctcaactc
     2881 ggtatccgcg tatagggaat cttgccgccg acatgtccgc cagccggtgg cttccgttcg
     2941 ctcaggctga tgacggtgcg ccccgagagc gcaatgagtc cgtcgatcgc tgctgatgcc
     3001 cgttccctgc gttgccgccc ggcatcgtcg tcgaacagca aatcaagctg gctggcgtca
     3061 ggtcgaatag tgtcactccg acgcgaaata ccttcacgcc gcacggattg gcttgtgcca
     3121 atttgcgcca aagggcgtcg atcccggcaa gccctgccat ctggactccg accgacagtc
     3181 ccgaactcag cccgcttcgg cgcgaaacgc catgctgact gcctgatcac tccgccggac
     3241 agattcacca cttttgtgtt gtgacgccta ctcctatggc tcgccccctt cttttcgtaa
     3301 cgcctgcaga tcgtcctgat cttcacgctc atctgcatag gcttcatcag cgcctgactg
     3361 gcgagcacga ttccggccct agccgcagga tcgcgcagga cgacctagtt acgaaccagg
     3421 ccgagtgttc ctgacatcca tcggcaccat agttctgctt atccggcacg cgcgcgtcag
     3481 gggtgtggtg aactacaaag cagacccgtt gaggttatca gttcgatgca caatcagcag
     3541 cgcataaaat atgcacaaga acaggagcac ccttcgcatt aagctgtggt ggtaacaagt
     3601 agtgccgggc taccatcagc gagcatgatg cgctcccaca gcattcgcct tggcagtatg
     3661 gaagttcctc gctccagttc gggccggtat ccacctcgag tgaccccagc cgcccctcat
     3721 gccaaccgag cccattaagc gcagagtcgg ccgcaactat ctcggggacg cataaacacg
     3781 cagcgattta ccaaggagtt cggctaggtc aatccgcaat tccctttgcc tggccggaga
     3841 tgccgcgagc aaacaatgtg tccggtcgct accagatgga ctttgtgggc ggaggattgc
     3901 accagacgct tcagcgcagc gtcgtacgat gaatcggaag gcccggtaag caattgaaga
     3961 accgctgacc acctcccatc cgacaatgca agggtgcccc gctcccagcg cgacacggtt
     4021 gcctgatcca cacccattaa ttcagctagg tgactttgct tcatatgacg aagcaaccgc
     4081 gccctccgca cctgccgccc cctgtcattc gtcaacattc ttcagcacct caatgtcgtt
     4141 cgtagtgagc ctcatttttt caagaccgcc gatgatgaga gccaggcctc gctcgaatgc
     4201 cgcgtccgga ccgccttcgt agacgatttt catcgcgctc tgtaggcgcg ccggcatcgt
     4261 agacgctgag gtggtcaact gatcttcgcc ccgctcctcg gcgtctgcct cgctagcttg
     4321 ctgctcaaga acagcgccga cggtgaagta gctgattgcc atcaacgcat aggtcgcgtc
     4381 acctgccgaa aagccagcat cgcaaaggaa gcgaagctgc gcgtcggctt tttccatctg
     4441 cggcgcggct ggccgcgtcc cggcatgaat acgcgcgcca tcgcgataag cgagcaacgc
     4501 ccgtcgaaaa ctgcatgcat tgcccttcag gaacgaacgc cagtcgtcgt catcccttgg
     4561 cgtcgaatgc gtgtgattta tcgtcagcat ggcttcggca agtgcgtcga gcaacgcacg
     4621 cttgttcttg aaatgccagt agagcgctgg ctgttgcacc ccgaggcgct cagccagtcg
     4681 gcgcgtcgtt agaccttcca tgcccacgtc gttaagcagt tcgagcgcgg ttcggatcac
     4741 ggcctcgcgt tggagcttgt tcattcgcga attctcatgt ttgacagctt atcatcgata
     4801 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca cccgcggttc
     4861 ggatcacggc ctcgcgttgg agcttgttca ttcgcgaatt ctcatgtttg acagcttatc
     4921 atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcacct
     4981 gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag
     5041 gcataggctt ggtttgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
     5101 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat tttctatgcg
     5161 cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcagcttcg
     5221 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc
     5281 tacgccggac gcatcgtggc cggcatcacc gtgggccaca ggtgcggttg ctggcgccta
     5341 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     5401 tttcgggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     5461 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     5521 tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc
     5581 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     5641 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     5701 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     5761 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     5821 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     5881 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     5941 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     6001 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
     6061 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     6121 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     6181 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     6241 gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg cgtccgccat
     6301 ctccagcagc cgcacgcggc gcatctcggg cagcgttggg tcctggccac gggtgcgcat
     6361 gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca
     6421 gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac
     6481 ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa
     6541 gtcccctacg tgctgctgaa gttgcccgca acagagagtg gaaccaaccg gtgataccac
     6601 gatactatga ctgagagtca acgccatgag cggcctcatt tcttattctg agttacaaca
     6661 gtccgcaccg ctgccggtag ctattgacta tccggctgca ctagccctgc gtcagatggc
     6721 tctgatccaa ggcaaactgc caaaatatct gctggcaccg gaagtcagcg ccctgcacca
     6781 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     6841 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtgccgc cctatccctt
     6901 tgtgcagctt gccacgctca aaggggtttg aggtccaacc gtacgaaaac gtacggtaag
     6961 aggaaaatta tcgtctgaaa aatcgattag tagacaagaa agtccgttaa gtgccaattt
     7021 tcgattaaaa agacaccgtt ttgatggcgt tttccaatgt acattatgtt tcgatatatc
     7081 agacagttac ttcactaacg tacgttttcg ttctattggc cttcagaccc catatcctta
     7141 atgtccttta tttgctgggg ttatcagatc cccccgacac gtttaattaa tgctttctcc
     7201 gccggagatc gacgcacagc gttctgtgct ctatgatgtt atttcttaat aatcatccag
     7261 gtattctctt tatcaccata cgtagtgcga gtgtccacct taacgcaggg ctttccgtca
     7321 cagcgcgata tgtcagccag cggggctttc ttttgccaga ccgcttccat cctctgcatt
     7381 tcagcaatct ggctataccc gtcattcata aaccacgtaa atgccgtcac gcaggaagcc
     7441 aggacgaaga atatcgtcag tacaagataa atcgcggatt tccacgtata gcgtgacatc
     7501 tcacgacgca tttcatggat catcgctttc gccgtatcgg cagcctgatt cagcgcttct
     7561 gtcgccggtt tctgctgtgc taatccggct tgtttcagtt ctttctcaac ctgagtgagc
     7621 gcggaactca ccgatttcct gacggtgtca gtcatattac cggacgcgct gtccagctca
     7681 cgaatgaccc tgctcagcgt ttcactttgc tgctgtaatt gtgatgaggc ggcctgaaac
     7741 tgttctgtca gagaagtaac acgcttttcc agcgcctgat gatgcccgat aagggcggca
     7801 atttgtttaa tttcgtcgct catacaaaat cctgtctatc gtgagaatga ccagccttta
     7861 tccggcttct gtcgtatctg ttcggcgagt cgctgtcgtt ctttctcctg ctgacgctgt
     7921 ttttccgcca gacgttcgcg ctctctctgc ctttccatct cctgatgtat cccctggaac
     7981 tccgccatcg catcgttaac aagggactga agatcgattt cttcctgtat atccttcatg
     8041 gcatcactga ccagtgcgtt cagcttgtca ggctcttttt caaaatcaaa cgttctgccg
     8101 gaatgggatt cctgctcagg ctctgacttc agctcctgtt ttagcgtcag agtatccctc
     8161 tcgctgaggg cttcccgtaa cgaggtagtc acgtcaatta cgctgtcacg ttcatcacgg
     8221 gactgctgca cctgcctttc agcctccctg cgctcaagaa tggcctgtag ttgctcagta
     8281 tcgaatcgct gaacctgacc cgcgcccaga tgccgctcag gctcacggtc aatgccctgc
     8341 gccttcaggg aacgggaatc aacccggtca gcgtgctgat accgttcaag gtgcttattc
     8401 tggaggtcag cccagcgttc cctctgggca acaaggtatt ctttgcgttc ggtcggtgtt
     8461 tccccgaaac gtgccttttt tgcgccaccg cgctccggct ctttggtgtt agcccgttta
     8521 aaatactgct cagggtcacg gtgaataccg tcattaatgc gttcagagaa catgatatgg
     8581 gcgtggggct gctcgccacc ggctatcgct gctttcggat tatggatagc gaactgatag
     8641 gcatggcggt cgccaatttc ctgttggaca aaatcgcgga caagctcaag acgttgttcg
     8701 ggttttaact cacgcggcag ggcaatctcg atttcacggt aggtacagcc gttggcacgt
     8761 tcagacgtgt cagcggcttt ccagaactcg gacggtttat gcgctgccca cgccggcata
     8821 ttgccggact ccttgtgctc aaggtcggag tctttttcac gggcatactt tccctcacgc
     8881 gcaatataat cggcatgagg agaggcactg ccttttccgc cggtttttac gctgagatga
     8941 taggaggcca tcgtgtttta tcccgctgaa ggcgcgcacc gtttctgaac gaagtgaaga
     9001 aacgtctaag tgcgccctga taaataaaag agttatcagg gattgtagtg ggatttgacc
     9061 tcctctgcca tcactgagca taatcattcc gttagcattc aggaggtaag cagcatgaat
     9121 aaaagcgaaa aacaggaaca atgggcagca gaaagagtgc agtatattcg cggcttaaag
     9181 tcgccgaatg agcaacagaa acttatgctg atactgacgg ataaagcaga taaaacagca
     9241 caggatatca aaacgctgtc cctgctgatg aaggctgaac aggcagcaga gaaagcgcag
     9301 gaagccagag cgaaagtcat gaacctgata caggcagaaa agcgagccga agccagagcc
     9361 gcccgtaaag cccgtgacca tgctctgtac cagtctgccg gcttacttat catggcggga
     9421 ctggtagaca gccagacagg gaagcccgtc gatgatactg ctgccctact gggcgcatta
     9481 gccagtctca atgatttgtc acgggataat cctaagtggt ctgactggaa aatcagaggt
     9541 caggaactgc tgaacagcaa gaagtctgac agctctgcat agcagacctg ccataaaacg
     9601 ccctgagaag cccgtgacgg gcttttcttg tgtcatgggt agatttcctc gtgtgagtcc
     9661 atgaaaagcg cctgtagcat catttacccc cattccctgc cagagctgtg agcgtagcga
     9721 actgaatgtc acgaaaaaga cagcgactca ggtgtctgat ggtcggagac gaaaggaata
     9781 ttcagcgatt tgcccgagct tgcgagggtg ctacctcatt ctttcgtgtt tgaaggtatg
     9841 ttttttggag gagcaaacgc tgtttgcgac atccttttat catatcgctc aactggcact
     9901 ggcggttagt tatccacaaa gctgggatct attctttttt atcttttttt tattctttct
     9961 ttattctagt aattttaata ttttgtttta taagtaaaaa acacatgaaa ggttaagcaa
    10021 aaattaggaa aggttaagca aaaattagga tctcccttca aaaggttaag tgaaaattag
    10081 ggaatccaca caaccacata aaaggttatg acaatgaaat tgacataacc aagcggaagg
    10141 gttatgttgt tttaaaatca caaccaatgg ttttagtcat ggctgaattg gtagtattca
    10201 aagcaaacga gcttgcggtt agtcgttatg acctaacaga gcacgaaacg aaactaatcc
    10261 tgttttgtgt tgcaaagcta aaccctacat tagaaacacc agatgaaact gaacgaacgg
    10321 ttaaattttc atgctccgaa tattcttcat ttatggggat aagttatgag aatgcgtggg
    10381 ggcgattaaa tacatcaaca cgagagttat ttaagcggtc tattgaatta atatatccaa
    10441 caggtgctgt atct
//
