LOCUS       NZ_CP051321             9588 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Muenster strain CVM
            34526 plasmid p34526-2, complete sequence.
ACCESSION   NZ_CP051321
VERSION     NZ_CP051321.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14504890
            Assembly: GCF_016452085.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Muenster
  ORGANISM  Salmonella enterica subsp. enterica serovar Muenster
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 9588)
  AUTHORS   Zhao,S., Li,C., Hsu,C.H., Tyson,G.H., Strain,E., Tate,H., Tran,T.T.,
            Abbott,J. and McDermott,P.F.
  TITLE     Comparative Genomic Analysis of 450 Strains of Salmonellaenterica
            Isolated from Diseased Animals
  JOURNAL   Genes (Basel) 11 (9), 1025 (2020)
   PUBMED   32883017
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9588)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 318x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:16:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,010
            CDSs (total)                      :: 4,886
            Genes (coding)                    :: 4,742
            CDSs (with protein)               :: 4,742
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 58 of 144
            Pseudo Genes (incomplete)         :: 100 of 144
            Pseudo Genes (internal stop)      :: 21 of 144
            Pseudo Genes (multiple problems)  :: 31 of 144
            Pseudo Genes (short protein)      :: 1 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051321.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9588
                     /organism="Salmonella enterica subsp. enterica serovar
                     Muenster"
                     /mol_type="genomic DNA"
                     /strain="CVM 34526"
                     /serovar="Muenster"
                     /isolation_source="turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:82689"
                     /plasmid="p34526-2"
                     /geo_loc_name="USA:NC"
                     /collection_date="2004"
                     /collected_by="NC State Veterinary Diagnostic Lab"
     gene            join(9360..9588,1..611)
                     /locus_tag="HFT84_RS23315"
                     /old_locus_tag="HFT84_23315"
     CDS             join(9360..9588,1..611)
                     /locus_tag="HFT84_RS23315"
                     /old_locus_tag="HFT84_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(1238..1375)
                     /locus_tag="HFT84_RS23320"
                     /old_locus_tag="HFT84_23320"
     CDS             complement(1238..1375)
                     /locus_tag="HFT84_RS23320"
                     /old_locus_tag="HFT84_23320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_327329051.1"
                     /translation="MSFRSTERQTPIRDEQERQAQELARRWHEEQEPEARIWRGPSMGM
                     "
                     /name="hypothetical protein"
     gene            complement(1491..2351)
                     /locus_tag="HFT84_RS23325"
                     /old_locus_tag="HFT84_23325"
     CDS             complement(1491..2351)
                     /locus_tag="HFT84_RS23325"
                     /old_locus_tag="HFT84_23325"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(2534..3091)
                     /locus_tag="HFT84_RS23330"
                     /old_locus_tag="HFT84_23330"
     CDS             complement(2534..3091)
                     /locus_tag="HFT84_RS23330"
                     /old_locus_tag="HFT84_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            3255..6260
                     /locus_tag="HFT84_RS23335"
                     /old_locus_tag="HFT84_23335"
     CDS             3255..6260
                     /locus_tag="HFT84_RS23335"
                     /old_locus_tag="HFT84_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            complement(6294..6527)
                     /locus_tag="HFT84_RS23340"
                     /old_locus_tag="HFT84_23340"
     CDS             complement(6294..6527)
                     /locus_tag="HFT84_RS23340"
                     /old_locus_tag="HFT84_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6534..7094)
                     /locus_tag="HFT84_RS23345"
                     /old_locus_tag="HFT84_23345"
     CDS             complement(6534..7094)
                     /locus_tag="HFT84_RS23345"
                     /old_locus_tag="HFT84_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<7126..7782)
                     /locus_tag="HFT84_RS23350"
                     /old_locus_tag="HFT84_23350"
                     /pseudo=""
     CDS             complement(<7126..7782)
                     /locus_tag="HFT84_RS23350"
                     /old_locus_tag="HFT84_23350"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7772..8095)
                     /locus_tag="HFT84_RS23355"
                     /old_locus_tag="HFT84_23355"
     CDS             complement(7772..8095)
                     /locus_tag="HFT84_RS23355"
                     /old_locus_tag="HFT84_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8158..8349
                     /locus_tag="HFT84_RS23360"
                     /old_locus_tag="HFT84_23360"
     CDS             8158..8349
                     /locus_tag="HFT84_RS23360"
                     /old_locus_tag="HFT84_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9019..9168
                     /locus_tag="HFT84_RS25195"
     CDS             9019..9168
                     /locus_tag="HFT84_RS25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     ARG             complement(1495..2351)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(8438..8522)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          8533..9088
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.907"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6293..7782)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.799"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taataaaaaa catatcatat tcactgatgg ctgcatttat ttttgataca gggcttaatt
       61 ttagcaagga gaatataact aaaggggtga tttcaacaag gtggcataat gacctatatt
      121 catcatttga gagaatgaaa gcaattaata aaatttacta cccatccaac aaagagataa
      181 acacagaagg tctatccaaa gccatcacat catctctttt caatgatgac gcgaactcgt
      241 tcgcaaagag agattttcgc ttgatgtggg acttatcttc tgaaaaatat ctttcctata
      301 aagaaataat aataagaaaa ggcgataagc ttgacgctgt atgcttgaga tttataaatg
      361 atgactacaa gtttttagtt aatttcaaca gagatgaaga ggttttcaaa tactttccat
      421 ctataatgca accttcactc aaaacatata gagcgttatc aagactagta aactcaatca
      481 aagatccttc tagatttaag ttcacgacag agtcattaga aatggagtta ctagaatact
      541 tagagttaag aaatgaatta ttcaatgaca tagaagaagt aatgggtagc tatgctcaga
      601 gggcaccata gttcatgata gccctcgata gtacttactt atatttttag agaggtttaa
      661 gtgtaagtag agggagtaac acctggtttt tcagcatcca ctccaatgaa tgctgacacc
      721 gatacgcgat aattctcttt atcgtagcaa aatcatcctc cagattgcgg ttgagaatag
      781 gttcatggtg cgcaatacga ttgcgcagtt ttctcacggg acttgttagc agtaatgctt
      841 ccccccgggg acaaacgaca gagatataca acaaaggccc gctgtataga gtacagcggg
      901 cctttgttgt tttaattccc gcaattaaaa tactccgtat ttaccataac gtcgcattgc
      961 gcgtaccgct taaacgcgtt cagcgcgatc acggcagcag acaggtaaaa acagcaacaa
     1021 accacccgaa aaactgccgc gatcgcgcct ggtaaatttt aacctcatgc atagttatgc
     1081 gcgaaactga atgacgatca gtttcgcacg ttccagccgt cgaacggggc aaaatgggga
     1141 ggtttggata ttgttaatgc taatgtacgt acattaacat tggcgccatc cgggtagggc
     1201 gtgccacaaa ctggctcgat ttcagggggc accccggtca catccccatt gacgggccac
     1261 gccagatccg cgcttcgggt tcctgctctt catgccagcg ccgggcaagc tcctgcgcct
     1321 gacgctcctg ttcatccctt atcggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1441 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1501 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1561 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1621 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1681 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1741 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1801 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1861 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1921 ttcggtcctc cgatcgttgt cagaagtaag ttggcagcag tgttatcact catggttatg
     1981 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2041 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2101 gcgtcaacac gggataatac cgcaccacat agcagaactt taaaagtgct catcattgga
     2161 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2221 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2281 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acgaaaatgt
     2341 tgaatactca tactcttcct ttttcaatat tattgaagca tttaccaggg ttattgtctc
     2401 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2461 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2521 aaaaataggc gtatcacgag gccctttcgt cttcaagaat tttataaacc gtggagcggg
     2581 caatactgag ctgatgagca atttccgttg caccagtgcc cttctgatga agcgtcagca
     2641 cgacgttcct gtccacggta cgcctgcggc caaatttgat tcctttcagc tttgcttcct
     2701 gtcggccctc attcgtgcgt tctaggatcc tccggcgttc agcctgtgcc acagccgaca
     2761 ggatggtgac caccatttgc cccatatcac cgtcggtact gatcccgtca tcaatgaacc
     2821 ggactgccac gccctgagcg tcaaattcct ttatcagttg gatcatatcg gcagtgtcgc
     2881 ggccaagacg gtcgagcttc ttaaccagaa tgacatcacc ttcctccacc ttcatcctca
     2941 gcaaatccag cccttcccgg tctgttgaac tgccggatgc cttatcggta aatatacggt
     3001 ttgctttcac acctgcgtct ttgagtgctc tgacctgaag atcaagagac tgctgactgg
     3061 ttgagacccg agcgtaacca aaaagtcgca taaaaatgta ccttaaatcg aatatcggac
     3121 aactcatgtc tattattaca aatttacgat ttaatagaca tattaatgta acagttttac
     3181 gatgtccgat aatttataac atttcgtacg gttggaaaaa tgttactaaa tgcccgtcag
     3241 gcagggaggc cgatatgccc gttgactttc tgaccactga gcagactgaa agctatggca
     3301 gattcaccgg tgaaccggat gagcttcagc tggcacgata ttttcacctt gatgaagcag
     3361 acaaggaatt tatcggaaaa agcagaggtg atcacaaccg tctgggcatt gccctgcaaa
     3421 ttggatgtgt ccgttttctg ggcaccttcc tcaccgatat gaatcatatt ccttccggcg
     3481 tccggcattt taccgccaga cagctcggga ttcgtgatat caccgttctt gcagaatacg
     3541 gtcagaggga aaatacccgc cgtgagcatg cagcgctgat acgtcagcac tatcagtatc
     3601 gtgaatttgc ctggccctgg acatttcgcc ttacccgtct tttatatacc cggagctgga
     3661 taagcaacga acgtcctggc ctgcttttcg atctggcgac agggtggctt atgcaacatc
     3721 gtattattct ccccggagcc actacgctga cccggttgat ttcagaggta agggaaaagg
     3781 cgacgttgcg cctgtggaac aaactggcac tgataccgtc agccgaacag cgttcacagc
     3841 tggagatgct gctggggcca actgattgca gccgcctgtc tttactggaa tcactgaaaa
     3901 agggccctgt gaccatcagt ggtccggcgt ttaatgaagc aattgaacgc tggaaaactc
     3961 tgaacgattt tggcctgcat gctgaaaacc tgagtacact cccggctgtg cgcctgaaaa
     4021 atctcgcacg ttatgctggt atgacttcgg tgttcaatat tgccaggatg tcaccgcaga
     4081 aaaggatggc ggttctggtt gcctttgtcc ttgcatggga aacgctggcg ctggatgatg
     4141 cattggacgt tctggacgcc atgctggccg ttatcatccg tgacgccaga aagattgggc
     4201 agaaaaaacg gctccgctcg ctgaaggatc tggataaatc tgcattggcg ctcgccagcg
     4261 catgttcgta cctgctgaaa gaagaaacac cggacgaatc gattcgtgct gaggtgttca
     4321 gctacatccc aaggcaaaag ctggctgaaa tcatcacgct tgtccgtgaa attgcccggc
     4381 cctcagacga taattttcat gaagaaatgg tggagcagta cgggcgcgtt cgtcgtttcc
     4441 tgccccatct gctgaatacc gttaaatttt catccgcacc tgccggggtt accactctga
     4501 atgcctgtga ctacctcagc cgggagttca gctcacggcg gcagtttttt gacgacgcac
     4561 caacggaaat tatcagtcgg tcatggaaac ggctggtgat taacaaggaa aaacatatca
     4621 cccgcagggg atacacgctc tgctttctca gtaaactgca ggatagtctg aggcggaggg
     4681 atgtctacgt taccggcagt aaccggtggg gagatcctcg tgcaagatta ctacagggtg
     4741 ctgactggca ggcaaaccgg attaaggttt atcgttcttt ggggcacccg acagacccgc
     4801 aggaagcaat aaaatctctg ggtcatcagc ttgatagtcg ttacagacag gttgctgcac
     4861 gtctttgcga aaatgaggct gtcgaactcg atgtttctgg cccgaagccc cggttgacaa
     4921 tttctcccct cgccagtctt gatgagccgg acagtctgaa acgactgagc aaaatgatca
     4981 gtgatctact ccctccggtg gatttaacgg agttgctgct cgaaattaac gcccataccg
     5041 gatttgctga tgagtttttc catgctagtg aagccagtgc cagagttgat gatctgcccg
     5101 tcagcatcag cgccgtgctg atggctgaag cctgcaatat cggtctggaa ccactgatca
     5161 gatcaaatgt tcctgcactg acccgacacc ggctgaactg gacaaaagcg aactatctgc
     5221 gggctgaaac tatcaccagc gctaatgcca gactggttga ttttcaggca acgctgccac
     5281 tggcacagat atggggtgga ggagaagtgg catctgcaga tggaatgcgc tttgttacgc
     5341 cagtcagaac aatcaatgcc ggaccgaacc gcaaatactt tggtaataac agagggatca
     5401 cctggtacaa ctttgtgtcc gatcagtatt ccggctttca tggcatcgtt ataccgggga
     5461 cgctgaggga ctctatcttt gtgctggaag gtcttctgga acaggagacc gggctgaatc
     5521 caaccgaaat tatgaccgat acagcaggtg ccagcgaact tgtctttggc cttttctggc
     5581 tgctgggata ccagttttct ccacgcctgg ctgatgccgg tgcttcggtt ttctggcgaa
     5641 tggaccatga tgccgactat ggcgtgctga atgatattgc cagagggcaa tcagatcccc
     5701 gaaaaatagt ccttcagtgg gacgaaatga tccggaccgc tggctccctg aagctgggca
     5761 aagtacaggt ttcagtgctg gtccgttcat tgctgaaaag tgaacgtcct tccggactga
     5821 ctcaggcaat cattgaagtg gggcgcatca acaaaacgct gtatctgctt aattatattg
     5881 atgatgaaga ttaccgccgg cgcattctga cccagcttaa tcggggagaa agtcgccatg
     5941 ccgttgccag agccatctgt cacggtcaaa aaggtgagat aagaaaacga tataccgacg
     6001 gtcaggaaga tcaactgggc acactggggc tggtcactaa cgccgtcgtg ttatggaaca
     6061 ctatttatat gcaggcagcc ctggatcatc tccgggcgca gggtgaaaca ctgaatgatg
     6121 aagatatcgc acgcctctcc ccgctttgcc acggacatat caatatgctc ggccattatt
     6181 ccttcacgct ggcagaactg gtgaccaaag gacatctgag accattaaaa gaggcgtcag
     6241 aggcagaaaa cgttgcttaa cgtgagtttt cgttccactg agcgtcagac cccttatctg
     6301 ttgttcgtga taaataaccg actcaagcgg tcgacttgtc cggctaatag ctgcacctgc
     6361 tgactcaagt tcacgacacg ctcgttcagt atcgcgttgt tctgttgcgt aaccgcgtgc
     6421 atcttctgca attctgcgaa ggcgctctcc cactcgttca gccgccgcat atagtcctgt
     6481 tgcaaatgct ctaaggcgtt cagcaaatgc ccttccagtt ccgtcatact cgtttactcc
     6541 ctgaccagcc tcacggcgtt cttgtcgccc accgtccagg tgttttcccc tttcaccccg
     6601 tccggcagca ccagaaattt cccgttctcg ccattgacga acgtcacccc ccaggttttt
     6661 gcctcgagtt tcgccagcgt gttttcctgc tctctgatgg tcaagatatt tttcgcaatc
     6721 ctgctttcct gccactgaat cagcccccag caccccagga aaatgccgcc cgtgacgctg
     6781 agcgcggtca gcgccggaaa cagtacccgc cctttgatca cctgccagag caggtttttc
     6841 tgctgccggg acagttcggc ctctgtggtg ctgagctgcg tgctcacggt ggtgctcagc
     6901 gtatccagtt gctgtttcac ggatgttgta tgttcgctga tagcgtctct gattttctgc
     6961 ctgtttaagc tcagtgcctt gtctacagac tcttctagct tcctgaactc gctgttcagc
     7021 atgttctcgg tagaagcggc tcgctctttc agtttcttct cgaagtctgt tcccatttgt
     7081 aaaagattgc tcatacagcg ccccctttag cctgagattc cgccccccct ccgggtcggc
     7141 aatactgata ctgcttctgg ttgtcctcac cacctcaaaa cctgccgctg tgagcgcctc
     7201 agtgacatcc tgacgcgttt ttattgctcc ggcacggtaa agcgcctcaa gaccccgtgt
     7261 aatcgcttct gcggcctcct gtttcgcttt tggcagatta ttcggtgtga caagcgtccg
     7321 tctgttctcc ggtgcgttcg ggtcacgcag cccgtaatga tgattaacca gcgtctgcca
     7381 ggcatttatc ctcggcctgt ccgcacggtc gtagtacggc tggaggcgct taccgctctg
     7441 tagttccatg ttcggaatga caaaattcag ttcgagccgc cccttatcct ggtgttccac
     7501 ccagagaatg ctgtactggt ctttttcgag accgggcata agaacacgct caaagctcgt
     7561 catcactctt tcgcgttcac ccggcggtaa ctccttctcc gcgaacgaca gaacgcctga
     7621 cgtgtatttc ttcgaaaatg gcgtggcatc aatgagttcc cggatttctt ccgggttacc
     7681 ccggagcacc gttgcgcctt cgcggttacg ctcccgcccc agcaggtaat cgaccgggcc
     7741 acttccaccg cctttccccc tggcatgaaa tttaactatc atcccgtccc ccctgttccc
     7801 tgacagcctg ccgcaactgg cgcaattcac cctcgatggc catcagtgcg gccaccacat
     7861 gaacccggtc aatcgaagac cagtggccgc tgttcacctt ccgggctgtc tggttcagat
     7921 tgttcccgat ggcagccaga tgccgcagca atggcggcga tagtgtcggc agttttcctg
     7981 tgcgcgcaac cggctccccc agacagaccc gccgcatcca ttctgccagt cgtttaccct
     8041 cgcagcgttc cagtaaccgg gcgtgctcct catcgctaac ccgtatcgtg agcatcttct
     8101 cgcgtttcat cggtatcatt ccccctaaaa aatcccctat taaggagcct taagaccatg
     8161 accaaacagg aaaaaacagc ccttaacatg gcccgcttta tcagaagcca gacgctgacc
     8221 ctgctggaaa aactcaacga gctggacgcg gacgaacagg cagacatctg cgaatcactt
     8281 cacgaccacg ccgacgaact ctaccgtagt tgcctcgcac gcttcggcga tgacggtgaa
     8341 agcaactgaa acatgcagct cccggagacg ttcacagctt gcctgtgagc ggatgccggg
     8401 agcagacaag cccgtcaggg cgcgtcagcg ggttttagcg ggtgtcgggg cgcagccctg
     8461 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgtggca tcagtgcgga
     8521 ttataagaaa agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     8581 gccgcatcag gcgctcttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc
     8641 tgcggcgagc ggtatcagct cactcaaaga cggtgatgcg gttatccaca gaatcagggg
     8701 ataacgccaa aagaaacatg tgagcaaaag gccaagaaca ggaagaaggc cgcgccgctg
     8761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaacagacg ctcaagtcag
     8821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8941 ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcggcccggt gtaggtcgtt
     9001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9061 ggtaactatc gtcttgagtc caacccggaa agacacgtaa aaacgccact ggcagcagcc
     9121 attggtaacc gtttagagga gttagtcttg aagttatgcg ccggttaagg ctaaactgaa
     9181 aggacagatt tggtggctgt gctcctccaa gccagttacc ttggttcaaa gagttgctgc
     9241 tctgagaacc ttcgaaaagc cgccttgctg ggcggttttt tcgtttacgg acaagactac
     9301 cttcaggatc gttagatctc aagacaatca tgcatagtga gcatacacag gagccgaact
     9361 tgcttacact gttcatcttt tttgttttat taatagctgc atgtttcttt tgttttgctc
     9421 caccacgaag aggatatgac agaaatgaga tcattccata caaaataaaa ctatcaataa
     9481 ataaatatcg cctttacata tactcatcgg gaaaggtaag acaatacctt ttatttttag
     9541 ttattttatc actgtactac tccatagcgg aaccatttaa gtctgagt
//
