LOCUS       NZ_CP051223            12565 bp    DNA     linear   CON 13-JUN-2023
DEFINITION  Escherichia coli strain SCZE5 plasmid pSCZE1.
ACCESSION   NZ_CP051223
VERSION     NZ_CP051223.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14558879
            Assembly: GCF_012537835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   He,J.
  TITLE     Structural analysis of Tn7-like transposons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   He,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2020) College of Life Sciences, Sichuan
            University, NO. 29 Wangjiang Road, Chengdu, Sichuan 610064, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 01:52:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,033
            CDSs (total)                      :: 4,918
            Genes (coding)                    :: 4,632
            CDSs (with protein)               :: 4,632
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 88 of 286
            Pseudo Genes (incomplete)         :: 208 of 286
            Pseudo Genes (internal stop)      :: 49 of 286
            Pseudo Genes (multiple problems)  :: 56 of 286
            Pseudo Genes (short protein)      :: 3 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051223.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCZE5"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pSCZE1"
                     /geo_loc_name="China:Sichuan"
                     /collection_date="2019-06-27"
     gene            <1..2220
                     /locus_tag="HF562_RS23345"
                     /old_locus_tag="HF562_23365"
     CDS             <1..2220
                     /locus_tag="HF562_RS23345"
                     /old_locus_tag="HF562_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="WP_168728576.1"
                     /translation="AGMAGLVGGLAALGVGKIIGAIADKIGQAQDNAIGLDKLYRQVGG
                     MVAYSNLRSGVGDIANRLGMNNADAIGMASTYTRASNLRPGMNLGTGLLVSGGLARGYG
                     LDPNSVAASFGSLGATGVTNSDQSIRRTGMMMGEAIAKSGAFAQAGDAMTAITDFANTQ
                     ARMSLSQPNIAGYGGALAGLMSSGVPGMDLQGAGSLLNRMNGAVMHGGNMGEASQAFSA
                     RLGLRRGINDPMALQALQEGGAFSTVSSMLGKGSLYNQTTGKSFSGNETMLDMIRHDIH
                     RQYGYGSNATKALANHLGIGVNEAMLVDQMSSRDINGASSRLKRLGIDEKSINMSSLPV
                     LGQIESGKGLEALGNQYLKRSDLSKEERRGLIDALKGANPEKLKDALAEVASKHGGTTT
                     EGSQIRDNIAKLNNTLEDYSKRALPALDMMRMALVNMTGGSEASVRKQYYDSNMREASS
                     EIGAKYKDRRAAIQSQIDALTAKGVGLQSSGPDREKLVKLKAEMQSLNDQEGADRTRAA
                     ERIKLQTYGASSSTEAALSTGDGYIQSPAAAAANGGGGKYRLNSDMKKRLERLRPYFEE
                     AGRKYNIDPRRLEAIAATESGFNNNLTSSKRAQGIMQVLPTSAPGANLSDERENIMAGA
                     MVYSQMRDRYGYLGQDEILRGYNGGSRRGSAENVAYPGKVNSYYRDLAQVDDMTPGSNG
                     NKGAVGEMYGTMRVVHEDKEGKQVKPPESVSMSGTFIQPSSWGNR"
                     /name="transglycosylase SLT domain-containing protein"
     gene            complement(2444..2998)
                     /locus_tag="HF562_RS23350"
                     /old_locus_tag="HF562_23370"
     CDS             complement(2444..2998)
                     /locus_tag="HF562_RS23350"
                     /old_locus_tag="HF562_23370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022319.1"
                     /translation="MGYFDCVKLTDAEWPDHMVEGRDAALRQAMRGGVSQWCRDKVVQL
                     LPVLRCPRGEWRDAELKRVDELADALGLDDMGLFAYGAMIVKRRAYVAGSLSRAKPPEP
                     LAVVLDDGEHRCTVYAMPHPTLGVPEISERGEDGYWTISAGGCAWIKARVSLGEGVRLW
                     NELVNCARYPDAPVLTRGMLE"
                     /name="hypothetical protein"
     gene            complement(2977..3273)
                     /locus_tag="HF562_RS23355"
                     /old_locus_tag="HF562_23375"
     CDS             complement(2977..3273)
                     /locus_tag="HF562_RS23355"
                     /old_locus_tag="HF562_23375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022318.1"
                     /translation="MSLYPKDKNPVFQPGGYGLRYDGQAEAIARAGEPPINYAPGQELS
                     RAAPALPEHPTHGAARAFRDHLTGEYVVAAGPVEVRTRVSAEEAALLWATLTA"
                     /name="hypothetical protein"
     gene            complement(3284..3652)
                     /locus_tag="HF562_RS23360"
                     /old_locus_tag="HF562_23380"
     CDS             complement(3284..3652)
                     /locus_tag="HF562_RS23360"
                     /old_locus_tag="HF562_23380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022317.1"
                     /translation="MGRVKGLPRHLQGKTRLPLLGGCFSKGHRLALLAVMPIIEARPGE
                     RFDGEAAKLALFQDLLLKAGQPPAFALHEPSLYRFGAALRNNRRGLTDTGIEKANELLD
                     QHLFSRALDAIVATKQTD"
                     /name="hypothetical protein"
     gene            complement(3640..4038)
                     /locus_tag="HF562_RS23365"
                     /old_locus_tag="HF562_23385"
     CDS             complement(3640..4038)
                     /locus_tag="HF562_RS23365"
                     /old_locus_tag="HF562_23385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249905.1"
                     /translation="MSMFDQLVARLRDEQALTKSLNPGDALAAVGNGDANAPVLDQRAL
                     TIGVAPGVVACHNARLNQIADAQLLQEVRASGVVSAADAVALDNALAASRHLPPEARAA
                     GMESVRQQLNAAAAAPLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            complement(4038..4724)
                     /locus_tag="HF562_RS23370"
                     /old_locus_tag="HF562_23390"
     CDS             complement(4038..4724)
                     /locus_tag="HF562_RS23370"
                     /old_locus_tag="HF562_23390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022316.1"
                     /translation="MENEQIAPVDFEGANRIYLADVLAEACSALPELPDLPVPSLDGDD
                     AAVASLLHLMRQRDEMASAIGVLNFELGKLKEAAEAAHDRLVDAEARIAAARDALLAGN
                     EPEPRDAMLALVVDQIRADVQAADAAVDIKSREVGALDAQMNTAVHQIEGAIDELICSC
                     RIARARALELALLNELRALAVHDGDKNRFNRAFVPAPELALLSSNYGQIEMLRPGRMPH
                     LNRGVL"
                     /name="hypothetical protein"
     gene            4989..5630
                     /locus_tag="HF562_RS23375"
                     /old_locus_tag="HF562_23395"
     CDS             4989..5630
                     /locus_tag="HF562_RS23375"
                     /old_locus_tag="HF562_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324021.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_039022315.1"
                     /translation="MITNTTAVAYMRASTQEQDANRAREEIALFAKEHGITIRGFYAEN
                     VSGTYLERPELNRLLEEAKPHDILLLEKLDRLSRLSAEEWSLLKERIRTKRLRIVCIDI
                     PTTLAVLQGGQPVGAGGFDVRRVVLDALNDMLLDIAAAWARDDYETRRKRQQQGIEKAK
                     KLGRFKGKQKDEELRSKIRTLLDTSSMSQNQIAQLIGCSRNTVSRVARGE"
                     /name="recombinase family protein"
     gene            5742..5933
                     /locus_tag="HF562_RS23380"
                     /old_locus_tag="HF562_23400"
     CDS             5742..5933
                     /locus_tag="HF562_RS23380"
                     /old_locus_tag="HF562_23400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022314.1"
                     /translation="MQSIQQTTPARYPGLSIENGEIRQHGKQRHCAANRKGQYQTTLRD
                     SAGRYQTVSILSLYAWEA"
                     /name="hypothetical protein"
     gene            5930..6127
                     /locus_tag="HF562_RS23385"
                     /old_locus_tag="HF562_23405"
     CDS             5930..6127
                     /locus_tag="HF562_RS23385"
                     /old_locus_tag="HF562_23405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_089503153.1"
                     /translation="MIMIPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQE
                     GKRPRIKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            6228..6578
                     /locus_tag="HF562_RS23390"
                     /old_locus_tag="HF562_23410"
     CDS             6228..6578
                     /locus_tag="HF562_RS23390"
                     /old_locus_tag="HF562_23410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022312.1"
                     /translation="MRTKAIIAAVLLATAGTAAAAPVATTYICNDGTFIHLNEQNGRLT
                     ASWRGADIPVTKIETGKLTASLNGEQGSSTLQLVVGYNTTPNPIAIITTVVNGSMNSQS
                     CKPDTASKHIDL"
                     /name="hypothetical protein"
     gene            6594..6836
                     /locus_tag="HF562_RS23395"
                     /old_locus_tag="HF562_23415"
     CDS             6594..6836
                     /locus_tag="HF562_RS23395"
                     /old_locus_tag="HF562_23415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022311.1"
                     /translation="MAFENIERRFQRWKVIMVPGAKSYESWDEMVTGENFPEEARYKYK
                     KHRTKAIDLTLKNPDYVHTMSENGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(7581..8876)
                     /locus_tag="HF562_RS23400"
                     /old_locus_tag="HF562_23420"
     CDS             complement(7581..8876)
                     /locus_tag="HF562_RS23400"
                     /old_locus_tag="HF562_23420"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_052249904.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKIGVDDCEPTSFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            9384..9911
                     /locus_tag="HF562_RS23405"
                     /old_locus_tag="HF562_23425"
     CDS             9384..9911
                     /locus_tag="HF562_RS23405"
                     /old_locus_tag="HF562_23425"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_052249903.1"
                     /translation="MQRKSPTFSLRLPADLLEQTNELAEKTNRTRTDVITDALRAYLGI
                     PEPQGESGNRLDLMVELLQDISNTLKHNVLQKPTGRATRPRAEKLSSNSSETQGAAQRP
                     AGAEISGHYDEAEVMATIRRMREEQRDRGFRYDNKAIAQALNEAGLLQSNGRPWNNDRI
                     NTVITRRMPDLK"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            complement(9971..10252)
                     /locus_tag="HF562_RS23410"
                     /old_locus_tag="HF562_23430"
     CDS             complement(9971..10252)
                     /locus_tag="HF562_RS23410"
                     /old_locus_tag="HF562_23430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249902.1"
                     /translation="MTDQTTTVADLALSQSAFLVSQAIVELDAMFGEGYAAAHPAVLAQ
                     FLQTAGVIYATQLQATAAFDLAQQLDRLIGLGLGVEAGLLQAAQGASH"
                     /name="hypothetical protein"
     gene            complement(10319..11458)
                     /locus_tag="HF562_RS23415"
                     /old_locus_tag="HF562_23435"
     CDS             complement(10319..11458)
                     /locus_tag="HF562_RS23415"
                     /old_locus_tag="HF562_23435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249901.1"
                     /translation="MVNERRDYLFDHAVFSAALARVDPDPSVMGWLAQARTLACWCELL
                     GMDPLETCVEWSARGVNAGKGDRAMLVSALRDLDELDRLAAAGAFLRSSGVAADVAAGR
                     IAVREPSEDELAARQAHLVALRERAAAAAEKLTEARIKATGRARMLLHRATKPGSAALY
                     WQAKERAACLPLPCPDDVKCSDWRTFLPVIGQVYWNSSLLVPLYGVNESLRLEQMSVEV
                     ICGRANPEAAGSLGEKRGLKNAPREGLFYPFDLDAVGSGAPVVVCEGFMSGLALSLLIG
                     GKLPVVCAMSVGNFGATVQSLNKFVMGGHPFVLVPDIGGQDLAIDQAIPNARVIDLSAV
                     PGAEGVDGYDPFDLLARHGVEMGRKYLRGLFREARDASL"
                     /name="hypothetical protein"
     gene            complement(11461..11640)
                     /locus_tag="HF562_RS23420"
                     /old_locus_tag="HF562_23440"
     CDS             complement(11461..11640)
                     /locus_tag="HF562_RS23420"
                     /old_locus_tag="HF562_23440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022310.1"
                     /translation="MKFRASAICDLMGVSRRQLDRWIKAGTFPLPDGRDAKGYWWEADR
                     VATRMISERKERGM"
                     /name="hypothetical protein"
     gene            12027..>12565
                     /locus_tag="HF562_RS23425"
                     /old_locus_tag="HF562_23445"
                     /pseudo=""
     CDS             12027..>12565
                     /locus_tag="HF562_RS23425"
                     /old_locus_tag="HF562_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324017.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /name="lytic transglycosylase"
ORIGIN
        1 gcgggcatgg cgggtcttgt cggcggtctg gcggcgctgg gcgtcggcaa aattatcggc
       61 gctatcgctg ataagatcgg acaggcgcag gacaacgcca ttgggctgga taaactgtac
      121 cgccaggtcg gcggcatggt ggcgtacagc aacctgcggt caggcgtcgg cgacatcgcc
      181 aaccgtctcg gcatgaacaa cgcagacgcg atcggcatgg cgtctaccta cacccgcgca
      241 tccaatctgc gccccggcat gaacctaggg acagggctac tggtaagcgg cgggctggca
      301 cgcggttacg gactcgatcc gaacagtgtc gccgcctcct ttggttccct cggagcgaca
      361 ggcgtcacta acagcgacca gagcatccgt cgaaccggca tgatgatggg ggaagcaatc
      421 gccaagtctg gcgcgtttgc acaggctggc gatgcaatga cggcgatcac cgactttgcc
      481 aacacccagg cacgcatgag cctgtcacag ccaaatatcg ccgggtacgg cggcgcactg
      541 gccggactta tgagttccgg cgtcccgggc atggatctgc agggagccgg tagcctctta
      601 aaccgcatga acggagccgt catgcacggc ggcaacatgg gggaagcgtc acaggcattc
      661 agcgcccgtc tcggactgcg ccggggcatc aatgacccga tggcgctcca ggcactgcag
      721 gaggggggag cgttctccac cgtcagcagc atgctgggca aggggagcct ctacaaccag
      781 accaccggca agagcttcag cggcaacgaa accatgctcg acatgatccg tcatgacatc
      841 caccgccagt acggatacgg ctccaacgcc accaaggcac tggcgaacca cctcggcatc
      901 ggcgtcaacg aggccatgct cgtcgaccag atgagcagca gggacatcaa cggcgcgtcg
      961 tcccgcctta agcgcctcgg tatcgacgag aaaagcatca acatgagctc cctccccgtc
     1021 ctcgggcaga tcgagagcgg caaaggcctg gaggcactcg gcaaccagta cctcaagcgc
     1081 agcgacctgt ccaaagagga gcggcgcggg ctgatcgacg cccttaaagg cgcaaacccg
     1141 gagaagctca aagacgcgct cgcagaggtc gcctcgaagc acggcggtac caccaccgag
     1201 gggagccaga tccgcgacaa catcgccaaa ctcaacaaca ccctcgagga ctactcgaaa
     1261 cgcgccctgc cagccctcga catgatgcgc atggctcttg taaatatgac aggcggctca
     1321 gaagccagcg ttcgtaagca gtattacgac agcaacatga gagaagccag cagtgagatc
     1381 ggcgcaaagt acaaagaccg ccgtgccgcc atccaaagcc agatcgatgc cctgacagca
     1441 aaaggcgtcg gcctccagtc ctccggtccg gacagggaga agctggtcaa gctcaaggcc
     1501 gagatgcaga gcctgaacga ccaggaaggg gctgaccgca ccagagccgc agaacgcatc
     1561 aaactacaga catacggggc gtcgtcctcg acagaggcgg cgctgtctac cggtgacgga
     1621 tacatccagt caccagcggc agcagcagcc aacggtggcg gcggcaaata ccgcctcaac
     1681 agcgacatga agaaacgcct ggagcgtctg cgcccttact tcgaggaggc cggacgcaag
     1741 tacaatatcg atccacgtcg ccttgaggcc atcgccgcca ctgagtccgg gtttaacaac
     1801 aacctgacca gcagcaaacg tgcgcagggc atcatgcagg tactgccaac cagcgccccg
     1861 ggagccaacc tcagcgacga acgcgagaac atcatggccg gtgccatggt ctacagccag
     1921 atgcgtgacc gatacggata cctgggacag gacgagatcc tgcgcggata caacggaggt
     1981 agccgccgtg gcagcgcaga gaacgtcgcc taccccggca aggtgaacag ctactaccgc
     2041 gatctggcac aggttgacga tatgaccccg ggcagcaacg gcaacaaagg cgctgtcggc
     2101 gagatgtacg gcacaatgcg cgtcgttcat gaggacaagg agggtaagca ggtcaagccg
     2161 ccagagagcg tcagcatgtc cggcacgttc atccagccaa gcagctgggg caaccggtag
     2221 cggcgcagca gcgcccaaaa gagaggggct tacgcccctt tttttgtggt ggccagacgg
     2281 ggagattcac gccgcgacca gtaccaggta ggtgatgcca tgacctcggc aagtcggcgc
     2341 tttctggcgt gccctgcagc aacaccgcga acgcgtcgag cctcgacaac ggcgggaggg
     2401 atgaccacat gggggatcac cagacgggat acagcaaact cgctcattcc agcatccccc
     2461 gcgtcaggac cggcgcatca gggtaccggg cgcaattaac cagctcgttc caaagcctga
     2521 ctccttcccc gagcgagacg cgagccttga tccatgcgca accgccagcg gagatagtcc
     2581 aatacccatc ctcgccgcgt tcggagatct ccggcacgcc gagcgtcggg tggggcattg
     2641 catagacagt acaacgatgc tcgccatcat cgaggacaac agccagcggc tcgggcggtt
     2701 tagcccgaga gagactacca gcaacgtaag cccgccgctt cacgatcata gcgccgtagg
     2761 caaacagccc catatcgtcc agccccaggg catcagcaag ctcgtctaca cgtttcagct
     2821 cggcatcacg ccactcgccc cgggggcacc gcagcaccgg gagaagctgg acaaccttgt
     2881 cccggcacca ttgggagacg ccaccacgca tggcctggcg aagggcggca tcacgcccct
     2941 caaccatgtg atcaggccac tccgcgtcgg tcagctttac gcagtcaaag tagcccacag
     3001 cagcgcggcc tcctcagccg atacgcgggt acgaacctcg accgggcccg cggcgacgac
     3061 gtactcgccc gtcaggtgat cacggaacgc acgcgcagcg ccatgcgtcg ggtgctcagg
     3121 cagcgcagga gcagcacggg acagctcctg acccggggcg tagttgatcg gtggctcacc
     3181 ggcacgcgca atggcctcgg cctgaccgtc atagcgcaga ccgtagccgc caggctgaaa
     3241 cacgggattt ttgtctttcg ggtataagct cattttttaa cctctaatct gtttgtttag
     3301 tcgccacaat ggcatcaagg gcacgggaga aaaggtgctg gtcgagcagc tcattggcct
     3361 tctcgatgcc cgtatccgtc agcccgcgcc ggttgttccg cagcgcagca ccgaagcgat
     3421 acagggacgg ctcatgcagc gcgaacgcgg gcggttgccc cgccttaagc agcagatcct
     3481 ggaacagcgc cagcttcgcc gcctccccgt cgaaccgctc accaggtcgc gcctcgatga
     3541 tcggcatgac agccagcagc gccagccggt gccccttcga gaagcaacca cccagcaacg
     3601 ggagccgcgt cttgccctgc aggtgacgcg gcaagccttt tactcgtccc aattgatacc
     3661 ccgggcagca ggactgagag gagcagcagc ggcagcgttc agctgctgcc ttaccgattc
     3721 catgcccgca gcacgagcct caggaggcag gtgacgcgaa gcagccagcg cattgtcgag
     3781 cgccaccgca tcggcggcag acaccacacc agaggcgcga acctcctgca gcagctgcgc
     3841 gtcggcgatc tggttcaggc gggcgttatg gcacgccacc acaccaggag cgacaccaat
     3901 ggtcagcgca cgctgatcca gcacgggtgc attcgcgtcg ccattgccga ccgcagccag
     3961 cgcgtcgccc gggttaaggg acttggtcag agcctgttcg tcgcgcagac gagcaaccag
     4021 ttgatcgaac atggacatca cagcacccct ctattcaggt gaggcatacg ccccggacgg
     4081 agcatctcga tctggccgta gttcgacgac agaagcgcca gctcaggagc cggaacgaat
     4141 gcacggttaa agcggttttt gtcgccgtca tgcacagcca gagcacgcag ctcgttaagc
     4201 agagccagct caagagcacg agcccgggcg atacggcagg agcagatcag ctcgtcaatg
     4261 gcaccctcga tctgatgcac ggcggtgttc atttgcgcat cgagcgcccc cacctcccgg
     4321 ctcttaatat ccaccgcagc atcggcggct tgcacgtcag cgcggatctg gtcgacaacc
     4381 agcgccagca tcgcatcacg gggctcgggc tcgttgccag ccagcaaagc atcacgggcg
     4441 gcggcgatac gcgcctcagc atcaaccaag cggtcatgcg cagcttcggc ggcttccttc
     4501 agcttaccca gctcgaagtt gagcacaccg atcgcgctgg ccatctcgtc acgctgcctc
     4561 atcaggtgaa gcagggacgc aacagcagca tcatcgccat ccagcgacgg cacagggaga
     4621 tcaggcagtt cagggagggc agagcatgcc tcagcgagca cgtctgcaag gtagatccgg
     4681 tttgcgccct cgaaatcgac gggggcgatt tgttcgtttt ccatagttct ctctctcaat
     4741 ttagccggaa cggctacgcc tatcgttgcg tcacgacagg tctggagcca gcgcgggaga
     4801 gtagaaccgg taggcagcgc ggccttgccg caccccaaag gcggggcgtc gaaagcggca
     4861 cctttagagt acacgccacc tgagcaggaa aacgccgcaa aaaagacgcg atatttgcat
     4921 cttataagtt gagatgagta ggcttactgt gcatgatggt gacagggaag tttaagagga
     4981 caaccagcat gatcaccaac accaccgccg tcgcctacat gcgagcgagc acccaggaac
     5041 aggacgcgaa ccgcgcccgc gaggagatcg ccctcttcgc caaagagcac ggcatcacca
     5101 tccggggctt ttacgcggag aacgtgtccg gcacctacct ggagcgcccc gaactcaacc
     5161 ggctactgga ggaggccaag ccccacgaca tcctcctcct tgagaagctc gatcgcctga
     5221 gccgcctcag cgccgaggag tggagcctcc tgaaagagcg catccgcacc aagcgcctcc
     5281 gcatcgtttg catagacata ccgaccaccc tcgccgtcct gcagggaggc cagccagtag
     5341 gcgcgggcgg gtttgacgtt cgccgcgtgg tactcgacgc cctgaacgac atgctgctcg
     5401 acatcgccgc agcatgggcg cgggacgatt acgagaccag gcgaaagcgc cagcaacagg
     5461 gtatcgagaa agcgaagaaa ctcggacggt tcaaagggaa acagaaagac gaggagctgc
     5521 ggagcaagat ccgcaccctg ctggacacat cgagcatgag ccagaatcag atcgcccagc
     5581 tgatcggctg ttcacggaac accgtgagcc gggtcgccag aggcgagtaa gagagagcgc
     5641 ggcggcaaag gtggctccaa ccgcctaacc gccgcaaccc cagccccgcg atagcctgag
     5701 gaggtcagcg gggcatcaac cacccaacga aaggagctga catgcagagc atacagcaga
     5761 caacccctgc ccgctacccg ggcctgagca ttgaaaacgg agagattcgc cagcacggaa
     5821 agcagcgcca ctgcgccgcc aaccgtaaag gccagtacca gaccaccctg cgcgacagcg
     5881 ccggtcggta ccagacggtc agtatcctca gcctttacgc atgggaggca tgatcatgat
     5941 cccaggcaga acgcagatcc tgaaagccac cgccgacata gccaaggctc tcggtactga
     6001 caccgccgat gtagagcgcc acttcctgca gcacgccgtc gtcctcgcac ggctccggca
     6061 ggagggaaag cgcccccgca tcaaagcgtg gatggcagag aacatcggca ccccgtcagc
     6121 gatgtaagaa aaacttaaat gttaaggtaa aacaacatac ggttttactt acgcattaac
     6181 gaaaaccata cacccggggc gcgaggagcg cccccacagg acacagcatg agaaccaagg
     6241 ccatcatcgc cgcagtcctt ctcgccaccg caggaaccgc cgcagccgca ccagtcgcca
     6301 cgacctacat ctgcaacgac gggacattta tccaccttaa cgagcagaat ggcagactca
     6361 ccgccagctg gcgcggtgcc gacatccccg tcaccaagat cgagacaggc aagctaaccg
     6421 ccagcctcaa cggtgagcag ggcagctcaa cactgcagct cgtcgtcggg tacaacacca
     6481 cacccaaccc gatcgctatc attaccaccg tcgtcaacgg cagcatgaac agccagagct
     6541 gcaagccaga taccgcaagc aagcatatcg acctgtaacg caaggagaga gaaatggcat
     6601 ttgaaaacat cgagcgccgg ttccagagat ggaaggtgat catggttccc ggagcgaagt
     6661 catacgagtc atgggatgag atggtcaccg gtgagaactt ccccgaggaa gcccggtaca
     6721 aatacaagaa gcaccgcacc aaggcgatcg acctcacgct gaaaaaccca gactacgtcc
     6781 acaccatgtc ggagaacggg atcacagtca ggtaccggct cgaacgatgg cgctaaccct
     6841 acccaggagc gcggcgaaag ctgcgcccct accggtcaga aagcacgctc ccaccagaac
     6901 gcatcagagc cattctaagc gcctcaaccc cgaatcaacc caacgacacg gcaacacccc
     6961 atttaacccc gtgtaccccc tgaaagttag ggcaggattt gacgaaccgg taggcgcggc
     7021 gatgccgcct aagggcaatg cacggtgcca accatgatcc accgtgcata aaccacccgc
     7081 caacccccgc cagcactgga aactggcccc atttccctcc gggctttttt agctggagat
     7141 ccggcaggtg caaaccacgc accggtgaga gcgacaccag atccaaacac gacacgcaaa
     7201 gtgcatcccc agcggggtgc agctgcggcg cgtcgatttc cagatgatcc acccgttcca
     7261 cccgatccgg cacaggttca taaatagtaa aaaaaacaat taattgttca attagtgagc
     7321 atttaccccc tatttaacca atttattcat tttttgtatt taattagaaa ttagggtgga
     7381 acgggtggaa cacaacggtg aaagccttta aatacgcagc attaactgtt ccacccacgg
     7441 gtggaacatg ggtggaacgg gtggaacacc cgggtggaac aggtggtaca aaaacggcgt
     7501 gatgatccac ccatgatcca cccgggtgga tcaataaaaa aaggggagca tatagctccc
     7561 ctcttacgga aaagtaaccc ttacacgaca gcttgcgttc gacggaagta gccacgaact
     7621 aacgcaccgt ttatgcgctt cctgccaacc tcatagccca atcgggacat gatagccgcc
     7681 atgcgctgat gctgcttgtt gctggtatgg ccaatcatga gccctacctg agagaacaac
     7741 gccttagtcg gcacgaagct ggtcggttcg cagtcgtcga ccccgatctt gagcagctcg
     7801 gcgatctcgt cttcccacgg atcctcgacg cggaactcgt catgcacctt acgcgccagc
     7861 gtttcagcag cctcccacat caccccattc tcattgaaca ggtggagcgc ctcagcccac
     7921 agctgcgagc ggtttttctc gatggccgcg cagtccacgt ccccggaatt catgggcaaa
     7981 tagcggcggt tccccgtcga gtcattgagg atctgcttgt tgttcgtggt accgatgaac
     8041 accacgcggc gcgggtttcg caccttgaac tctttaaatt tcggcaccca ctcgtcgtgg
     8101 gcagcggaga agaacgcttt caccgattcg gagtcgcgcc cgtggaggcc gcgcagctcc
     8161 tccagctcgc caatcaggca cccacgcata cggcgcacta gggagtcgtc cttgtcgttc
     8221 aggttgaagc tggagtagga gtcagggaat ggcgacataa ggcgaacgcc cgtcgatttg
     8281 cgggcaccct gtcgcccgta cagcagcgga accatgtcgg ccttgattcc gggattcatt
     8341 acgcgcccgg caagcgccgt ccaggtgtag aggctgaccg ccgccaggta ctcgggatcc
     8401 ccttggcatt tgaagcaccg ctcgaagaag taactgacgc gaggtttgcc gtcccattcc
     8461 tgagccgtca gccaacgctg ggcgctgtcg aatttgttct catcgcagac gtagaacatg
     8521 gcgtcgcggg cattgtcctt ggtcaccttc tcaaagtcgg cagcagcgag gcgcagacgc
     8581 agctcggtaa ctaggttgtc ggacagcggg taccattgcc cgttcacgaa cgccccctcc
     8641 tccgcaaaga actcatcgaa agcgagctgg atgccggtga cggaatggca gcgcatagcg
     8701 gcgacaacgt tctcaagaga ggacgagtag cgccccttcc ggtagttgat ctccagctcc
     8761 acgggaaacg cggtagtcgg gtattttttt tgtagagacg ggcgtcccac cttggcagcg
     8821 gcgatcggcg ttacgttgtc ggcagcagtg ccggaatttt catcaatttg tgacacgcaa
     8881 ttactccttt aaaaacgtag gagtcggcgt caggcggggg cttacgaggg aaattcacac
     8941 ttgcgcgatc ttatcgacgt gatacaattc tcagcgagga tttgagaatc acccaacgtg
     9001 atgtatgatt tccttcgagt tcgtcccgtt aagacactga ctcgaaacta cggggtttaa
     9061 ccccggcaat caaagcaccc ggccaggtgc tttttttgta tccgcagatc acccaacacg
     9121 atccacgaac gacgatcagc ttaaccgatc ctaacccttc atgccactga acggcgaccg
     9181 cttatcgagc tgtcgtgatc gcgccgcgct tttatccact gcaacgtcaa actgtccaca
     9241 gggcttgtcc acaggcacca gagaggcgag ccgaagggct cgacccgtac cggttatgca
     9301 gcacacgcgc aacacgtcga aatagacgcg aggttacaaa cgtgtttcaa tcggtggcac
     9361 cagacaacac aggagcaaca cggatgcagc gcaaaagccc gacattcagc ctacgtctac
     9421 ccgccgacct gctcgagcag acgaacgagc tggccgagaa aacgaaccgt acccgcaccg
     9481 acgtgattac cgacgcactc cgcgcatacc ttggcatacc agagccgcag ggggagagcg
     9541 gcaaccgcct cgacctgatg gtggagctgc tgcaggacat ctcgaatacg ctgaaacaca
     9601 acgtgctaca gaagccaaca ggacgcgcta caaggccacg ggctgaaaag ctgagtagta
     9661 actcatctga aacacaaggc gcagcacagc gccccgctgg cgctgaaatt tcggggcatt
     9721 acgacgaggc cgaggtgatg gccaccatac ggcgcatgag ggaggagcag cgcgatcggg
     9781 gattccgcta cgacaacaaa gctatcgccc aggcgctgaa cgaggcaggg ctcctgcagt
     9841 cgaacggtcg cccatggaac aacgaccgca tcaacacggt gatcacccgc cggatgcctg
     9901 acctgaagta accggtagcg cggcagccgc cgcccagggc aaaggactga tcgcagccgg
     9961 ccccggcgtt ttagtgcgag gcaccctgcg ccgcttgcag caagccagcc tcaacaccca
    10021 gcccaaggcc gatcaggcga tcgagctgtt gggcgaggtc gaacgcggca gtagcctgca
    10081 gctgcgtggc gtagatcacc cccgccgtct gcaggaattg agccagcaca gccgggtgag
    10141 cagcggcata gccctcgccg aacatggcgt ccagctcgac gatagcctga gagacgagga
    10201 aagcggattg tgagagcgcc aggtcggcga cggtggtggt ctggtcggtc atggggagat
    10261 cctcaaataa aagcccgctg aagcgggctg ggcacaacga gagagaaaac tggtaccact
    10321 acaggctcgc gtcacgcgcc tcccgaaaca agccccgcag gtacttgcga cccatctcga
    10381 cgccgtgacg cgccagcaga tcgaacgggt cgtagccgtc cacgccctcg gctcccggta
    10441 ccgccgacag gtcgatcacc cgggcattcg ggatagcctg atcgatagcc agatcctgcc
    10501 cgccaatgtc cggtaccagt acgaacgggt gtccccccat aacaaactta ttcagcgact
    10561 gcaccgttgc cccgaagtta cccaccgaca tcgcgcagac caccgggagc ttgccgccaa
    10621 tcagcaggga gagcgccagc cccgacatga acccctcgca gaccaccaca ggagccccgg
    10681 aaccgacagc atccagatcg aacgggtaga acagcccctc acgcggcgca ttcttgagcc
    10741 cgcgcttctc gcccagcgaa cccgccgcct ccgggttagc ccgaccgcaa atcacctcga
    10801 ccgacatctg ctccagccgc agggactcat tcaccccgta cagcggcacc aggagcgagc
    10861 tgttccagta cacctgaccg attacaggca ggaacgtgcg ccagtccgag cacttcacgt
    10921 catccgggca cggcaaaggg agacacgccg cccgctcttt ggcttgccag tacagcgcag
    10981 cactccctgg tttggttgcg cgatgcagca gcatccgcgc cctaccggtt gccttaatgc
    11041 gagcctccgt cagcttctcc gcagcagccg ccgcacgttc ccgtaacgca accagatgcg
    11101 cctggcgagc cgccagctcg tcctcggacg gctcacgcac agcaatacgc cccgcggcaa
    11161 cgtcagcagc gaccccggag gagcgcagga acgccccagc agccgccagc cggtcgagtt
    11221 catccaggtc acgcagcgcc gacaccaaca tagccctgtc gccctttccc gcattcacac
    11281 cacgcgcaga ccactccaca caggtctcca gcggatccat ccccagcagt tcacaccagc
    11341 acgccagcgt gcgagcctga gccagccagc ccataaccga cggatccggg tctacacgcg
    11401 ccagcgccgc cgagaacacc gcgtggtcaa aaaggtaatc tcgtctctcg ttcaccataa
    11461 tcacatcccc cgttctttgc gttcggagat catccgcgtg gccacccggt cagcctccca
    11521 ccagtacccc tttgcgtcgc gcccgtccgg cagaggaaag gtgcccgcct tgatccagcg
    11581 atcgagctga cgacgggaca cacccatcag gtcgcagatc gcgcttgcac gaaacttcat
    11641 ggaaccccct gtagaaaagc cccgccgtga gtggtcgggg ctttgagtga tgactattgc
    11701 gatgcagtcc ttatgggctt atcgccggag cgacagggct gcgccattcg cgcttactcg
    11761 cttttcacca ggtggaagtg cgccaccgga agtagaccga agtccaggca cccgagcgat
    11821 ccgggccatg tttatccaac tgctattgga tggtgtctgt tttagactcg ttttagacat
    11881 tttacaagtt ctatttttca aagaatccag acgtggagcg gcttgcgcgt cgatttcgac
    11941 gcattgcgtt ttagacacgt tttagacatt taaccccaaa cgtcgtgacg ccagcatgag
    12001 gaggcttaca acagagagag ataactatgg ctatcaaaat tccggtctcg gcgcagttcg
    12061 acgctgccga cctccaacag cagatcaaaa tggtcaatga ccagatccgc attctggcca
    12121 gccaggtagg taacgccaac aagcagaagt gggaacccat caacctcaag agcaaggacg
    12181 acctgcaggg cttcatcaag cagatggact tgctcatgaa gaaacagact gactttgcca
    12241 ccaaaatgaa acagaccggg cagggcggta accccctgtt tgccgacttc aagaaaatgg
    12301 ccgaaggtcg cctcggagag caaatcaagt acatggagag cgtgctgggg tgggcgggcg
    12361 tagagttcag caacctgcca gcaccaaagc cccccaaaaa gccgcctgtc gtgcccccag
    12421 cgcctccgtc gccgccagaa cccgcgtggc gcaaagagct ggggagacag ggaatgagtg
    12481 gcctccacag catcctcggc aatgccggcc ctattggcgg tactatcagc aacgccctga
    12541 acaccggtat gtcttccggc gcgag
//
