LOCUS       NZ_CP051136             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain AMS-38 plasmid pAMS-38d, complete
            sequence.
ACCESSION   NZ_CP051136
VERSION     NZ_CP051136.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14492121
            Assembly: GCF_012562255.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Soliman,A.M.
  TITLE     Emergence of a Multidrug-Resistant Enterobacter hormaechei Clinical
            Isolate Co-Producing MCR-9 and VIM-4 from Egypt
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Soliman,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2020) Office of Academic Research and
            Industry-Government Collaboration, Academy of Hiroshima University,
            1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 166x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012562255.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:30:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,036
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 49 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 10 of 98
            Pseudo Genes (multiple problems)  :: 12 of 98
            Pseudo Genes (short protein)      :: 1 of 98
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051136.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="AMS-38"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pAMS-38d"
                     /geo_loc_name="Egypt: Cairo"
                     /collection_date="2015-04"
     gene            950..1093
                     /locus_tag="HDB52_RS25960"
     CDS             950..1093
                     /locus_tag="HDB52_RS25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1521..1748
                     /locus_tag="HDB52_RS25665"
     CDS             1521..1748
                     /locus_tag="HDB52_RS25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2115..2300
                     /locus_tag="HDB52_RS25670"
     CDS             2115..2300
                     /locus_tag="HDB52_RS25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2348..2427)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          555..1115
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 tccataacag catcatgcaa cgacgaccat cgtccgtgac ggccgcctcg tttaagtatg
       61 gacagaaata cagaaaatgc tcaggacgaa atgtaatgaa tgcgaacgga ttcaagaaat
      121 tcgagcatga ccgtccttac ggccggttca gtttcagaca aaatctgccg gtatgcatcc
      181 agcatcatgg ctccggcatc cccccggcac gccggagcca gcccgaaaca acagacacaa
      241 acaggtgtcg ctcatcatca agagtcatca ggtctccgga agaaaaacca aacatggctt
      301 acctcctgtt aacggcaacg cagaagaaag gtttacaggg tagcgggcag gaaaataaaa
      361 aaattgcagt cagcccggaa cggctgctga accggtacag gcgggttccg ggcgcagcgc
      421 cggaccagtc actcagcgct agcggagtgt atactggcta aaatgtaaca ccgcagtaac
      481 gcatagcata actgctacat aagacaggcg cagaaaatga aagaaaacgc gtcaggagaa
      541 aatgtaatac aggatatatt ccgcttcctc gctcactcac tcgctgcgct cggtcgttcg
      601 gctgcggcga gcggtacggg cttacgggtg gagcagaaat ttcctagggg atgccagcta
      661 ggggatgcca ggaagatact tagcgaagaa ggcataaggc cgcgccgaag ccgtttttcc
      721 ataggctccg cccccctgac gagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      781 acccgatgcg gcttaaagat accaggcgtt tccccctgat ggctccctcg tgctcccccg
      841 cattcctgcc tttcggttta ccggtgtcac tccgctgtta tggccgcggt ttctcattcc
      901 acgcctgaca ctcggttccg ggaaggcagt tcgctccagg atggactgta tgcacgaacc
      961 ccccgttcag tccgactgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga
     1021 aagacacgta aaaacgccac tggcagcggc cactggtaag gggtatacaa gattactggt
     1081 gcagagttct tgaagttgac gcctgactgc ggctacacta gaaggacaaa tttggtggca
     1141 gcggtcttgt acaccctgtt accacggttc agaaacttgc cggtttgctg aaccttcgaa
     1201 aaaccacctt gccgggtggt tttttcgttt acagggcaag agattacgcg cgaaaaaaaa
     1261 ggatctcaag aagatccttt tataaattag acatccaacc tcacaaagag gaatacttaa
     1321 atcatcttgg tatatgtatc ggtcggatta catcagatat aagcccgcac tgccccctcc
     1381 ccgccagaca ccacacttta accataacac ctcgcaccta actcgatatg cagcccacca
     1441 tcaagcctct tttcacataa aaaatgacct atagttagat ggttagtgac caaaactgat
     1501 atggataaaa ggagctggct ttgaaaaaga tttttgtttc cgtgttcgca gcggcagttg
     1561 cgctttcagc cctcaccggc tgcacgcgca caagctatgc catacacacc aatgacgggc
     1621 gcactattgt cagcgacggc aaacctaaag aatcagactc aggcctgctt ggctatacag
     1681 acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt taaagaagtc tccgaaattc
     1741 cgcactgagc atcttctccg aaaaaggccg ctaatgtggc ctttttcttt acctggggtt
     1801 ctccgccaaa atcccagcaa attgcaccac caaagctaaa agcttccagg gttgtctaaa
     1861 aagcacaaga cattagagga agtgcggttt ttataatcaa gccccccaag aatttaccat
     1921 aacatccgtt gcccgcaccg cctcagacgc actcagcgct tttctgacgg aaccatgacc
     1981 aaaaaccaca acaaaccacc ccggaacccg ccagaaacgc tcctgttaaa ttttaacggc
     2041 atgcatgact atgcaccaga atgacggcat gctcttttca cgcaaaaatc atcaccagac
     2101 ggggaaaatc accagtgacc agacaggaat ccgccgccct caacatggcc aaatttatcc
     2161 gctcacagac actcctcctc ctcgagcggc tcgaacagat ggatctggat gaggctgccg
     2221 gctgctgtga gcacctgcac gatcaggccg aagcacttta caccatgctg aacgcacaga
     2281 caggcgagga agatgcgtga aaatcggtga acgggtgcgc aattcagtgc gcggccgtga
     2341 ggcgatggcg gggtgtcggg gcgcagccct gacctgggta tttgtgatgc cggcgcgtgc
     2401 gcggtattac aaatacacat cctgtcctga gtggttcccc gggaacaggc aaaaaaaaac
     2461 cggggagctg cccggaaggt aagtcaagtt agattagatt actttcactc a
//
