LOCUS       NZ_CP051134             9522 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain AMS-38 plasmid pAMS-38b, complete
            sequence.
ACCESSION   NZ_CP051134
VERSION     NZ_CP051134.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14492121
            Assembly: GCF_012562255.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Soliman,A.M.
  TITLE     Emergence of a Multidrug-Resistant Enterobacter hormaechei Clinical
            Isolate Co-Producing MCR-9 and VIM-4 from Egypt
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Soliman,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2020) Office of Academic Research and
            Industry-Government Collaboration, Academy of Hiroshima University,
            1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 166x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_012562255.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:30:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,036
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 49 of 98
            Pseudo Genes (incomplete)         :: 52 of 98
            Pseudo Genes (internal stop)      :: 10 of 98
            Pseudo Genes (multiple problems)  :: 12 of 98
            Pseudo Genes (short protein)      :: 1 of 98
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP051134.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="AMS-38"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pAMS-38b"
                     /geo_loc_name="Egypt: Cairo"
                     /collection_date="2015-04"
     gene            232..375
                     /locus_tag="HDB52_RS25915"
     CDS             232..375
                     /locus_tag="HDB52_RS25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(596..871)
                     /locus_tag="HDB52_RS24090"
     CDS             complement(596..871)
                     /locus_tag="HDB52_RS24090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_168728694.1"
                     /translation="MLQKLSRGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEKSDN
                     FQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(1458..2371)
                     /gene="lpxO"
                     /locus_tag="HDB52_RS24095"
                     /pseudo=""
     CDS             complement(1458..2371)
                     /gene="lpxO"
                     /locus_tag="HDB52_RS24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /name="lpxO"
     gene            complement(2642..2857)
                     /locus_tag="HDB52_RS24100"
     CDS             complement(2642..2857)
                     /locus_tag="HDB52_RS24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2861..3346)
                     /locus_tag="HDB52_RS24105"
     CDS             complement(2861..3346)
                     /locus_tag="HDB52_RS24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(3359..3865)
                     /locus_tag="HDB52_RS24110"
     CDS             complement(3359..3865)
                     /locus_tag="HDB52_RS24110"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_240963622.1"
                     /translation="MSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGR
                     LELNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPS
                     ALPETKQEAAQAITRGLLALASSGELKTRPRTSLRRWKAQVLRWYAPQKAASALPTRTG
                     GETSD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3804..4037)
                     /locus_tag="HDB52_RS25920"
     CDS             complement(3804..4037)
                     /locus_tag="HDB52_RS25920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_240963623.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELID
                     ASPYAKKVHIRRPVLCRKGFTARAARKADGEL"
                     /name="hypothetical protein"
     gene            complement(4027..4350)
                     /locus_tag="HDB52_RS24115"
     CDS             complement(4027..4350)
                     /locus_tag="HDB52_RS24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4994..5104
                     /locus_tag="HDB52_RS25925"
     CDS             4994..5104
                     /locus_tag="HDB52_RS25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(5359..5820)
                     /locus_tag="HDB52_RS24125"
     CDS             complement(5359..5820)
                     /locus_tag="HDB52_RS24125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(6220..7134)
                     /gene="lpxO"
                     /locus_tag="HDB52_RS24130"
     CDS             complement(6220..7134)
                     /gene="lpxO"
                     /locus_tag="HDB52_RS24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(7405..7620)
                     /locus_tag="HDB52_RS24135"
     CDS             complement(7405..7620)
                     /locus_tag="HDB52_RS24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7624..8109)
                     /locus_tag="HDB52_RS24140"
     CDS             complement(7624..8109)
                     /locus_tag="HDB52_RS24140"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<8144..8848)
                     /locus_tag="HDB52_RS26020"
                     /pseudo=""
     CDS             complement(<8144..8848)
                     /locus_tag="HDB52_RS26020"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8787..9112)
                     /locus_tag="HDB52_RS24150"
                     /pseudo=""
     CDS             complement(8787..9112)
                     /locus_tag="HDB52_RS24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4452..4638)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          53..558
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.932"
                     /coverage="99.0"
                     /evalue="1.02e-159"
     Typing          4618..5104
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(2545..3464)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.026"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3468..4012)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.22"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4031..4350)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.64e-64"
     Typing          complement(7308..8797)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccataggctc cgcccccctg acgagcatca cgaaatctga cgctcaaatc agtggtggcg
       61 aaacccgatg cggcttaaag ataccaggcg tttccccctg atggctccct cgtgctcccc
      121 cgcattcctg cctttcggtt taccggtgtc actccgctgt tatggccgcg gtttctcatt
      181 ccacgcctga cactcggttc cgggaaggca gttcgctcca ggatggactg tatgcacgaa
      241 ccccccgttc agtccgactg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      301 gaaagacacg taaaaacgcc actggcagcg gccactggta aggggtatac aagattactg
      361 gtgcagagtt cttgaagttg acgcctgact gcggctacac tagaaggaca aatttggtgg
      421 cagcggtctt gtacaccctg ttaccacggt tcagaaactt gccggtttgc tgaaccttcg
      481 aaaaaccacc ttgccgggtg gttttttcgt ttacagggca agagattacg cgcgaaaaaa
      541 aaggatctca agaagatcct tttataaatt ggataatatc cctccgcccc taacactaat
      601 ataaaattat cgggcttgca tgcccctcac cattttcccc atatccacca ccaaacaata
      661 gttcgtcgag ttctttgcta gcttgatcaa tacacgcagc cacatgaata gcagaagggt
      721 actcatcata ctgaaaatta tcgctttttt caggagggag tcgatcataa tacggatgac
      781 aatccacata ataatgacgt attagcatcc cagttttatc ttcgtactct gatggctgca
      841 acgtactccc acgggaaagc ttttgcaaca tgtatagcag cttgaaaggt atcaaaatat
      901 cgcccaaaca ttggcgtttc aaagctaggc gactctgtcg aaaaaatccc ccataacttg
      961 cgccaaataa ccattatcta tctgccattg aacatctgat cccatagccc actctcccag
     1021 agttactcct aataaataac cataatatgt atttaccata acgcttcttg gccgcacccc
     1081 ctgagagccc ctcagcgcgt ttctgcggcc tgagggccgt ttccggcacc tttccccggc
     1141 ccaaaccctg aaaaacgccc ctgataattt ctgcgcggct gcatggctat gcacaagagt
     1201 gaatggcggc cggtttcgtg aaggaaatac cgtgaaagct ccgaaagaaa cgcttaattg
     1261 cacagcagca catgaacgtg atcagatgaa atctgtctac tgccgcactt cttcacgaaa
     1321 ggcccgctga gcgtcagatt cgggcctttc ttactaaaaa gatttcaata gaatttttta
     1381 gcaggcgatt tttatctata accatacatt gagcatagaa gacccaaatt tatgtatgac
     1441 tcaatagaaa aaacaaatca cagccatcta aacactgaga cagcaagcca aacaaaaatc
     1501 aaagaaataa ctccattttt aatcaaataa tatagttttc gattcctttt ttttagcttc
     1561 tgacctgcat cacgaatagc attaacatat ttaaataaat gatttatagc accagtcctg
     1621 tcattattat cgttgggtga tgctgccgct gacattagca ccccacctat ccacctattt
     1681 attttttgtg accaccgcca cttcatcggc ctttctatat cacagaacaa aataatacgt
     1741 gtctgctcgg tatcattctg agcccagtga acaaaagttt cgtcaaaaat tatcacttca
     1801 ccatcatgcc aactgcggcg tacaccatca acttcaataa aacaccgttc atcatttggt
     1861 gttgataatc ctaaatgata acgaactgaa ccggcataag ggtcacgatg ctttcctaaa
     1921 tggcttcctg aaggcaattc agcaaacatc gctgctttaa tacagggaat agagctaacc
     1981 agttgggttg ttactgggca aagccgctcc gctgatggat gagcatctcc ataccacttg
     2041 aggtaaaagc gcttccaacc gcgcttaaaa atgtattaaa tcctgcatca ttattttttt
     2101 cagatgattt aatgtgccct tgcaaattaa cagcttcgtc tcttataacc tgccagttat
     2161 caattaatgg ttgaagttca ggaaatgttt ctgggggaaa ccatgcctgc tttgacggca
     2221 gcttcgaaaa gcctgtcata aacatattta tcggagccat aaatgttgaa tgatcaaata
     2281 actgcctgga aaacttttgt tttattttac cgcgagaatg cgcatacaat acactgataa
     2341 gaaaaacaaa aataataata agtgacagca tgtgtatatt ccttgttcac gccaataata
     2401 aaaatctctt aaagataaca taatgaatgc acttaaatta taatctaaat gctatttatc
     2461 aatagtataa tcgaagcttc accagaacat atctactaca caggcagaga ggatttcagc
     2521 aggaattatg ctgaaatgaa tcaaagggac ggaccgtgat atacccgctg tcgctgtgat
     2581 cgttcgagct ccagcgcttt ctgatgccgc gcctcccgca cctgctgcag ctcgacggca
     2641 ttcagccgct cgacaaccgg cgcagctgat cgctcaaact ctgcacctgc ctgctcaagc
     2701 ccgtgacgcg ctcgctcagc gccgcgttct cccgttgcat aagaccagac atcgtccgcc
     2761 attccgcgaa ggcgctctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct
     2821 ctaatgcgct cagcaactgt ttttccagct ctgtcatgtg ctatttcccc gccagtatca
     2881 tccagctcga atcctctccg aaccgtcccg cttccgggtt caccctcacg cagcggcgtc
     2941 tctgctcgcc gcaggtcgag agctgcacgc cgctgttccg ctcggacagc atcgcctgcg
     3001 tgctcttctg ctcccggatg ctcacgtaat tgtcgagtat ctgctgactc tgccaccaca
     3061 gaatggccgc gttcgaggcg atcagcagca cggagaccag gacgatggtc agccacgtct
     3121 ggctgaccat gcgcagcatc cctttcgtac gctggctcat ggctgaggac agcttccggt
     3181 cgtggtcgag gatggcggcg ctgattctct tctcgctttc gctcagttcc gctctgacag
     3241 acttctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtactctgc tgctgcgctt
     3301 tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctcccttc
     3361 agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt tgtggtgcgt
     3421 accacctcaa aacctgcgct ttccagcgcc tcagtgacgt cctcgggcgg gttttcagct
     3481 ccccggacga ggccaaggcc agtaaacccc gtgtaattgc ctgagcggct tcctgcttcg
     3541 tttccggcag cgcggacggc gtgaccagcg cccgccggtt ttcaggcgcg ttcgggtcat
     3601 gcagccccag cctgccgttc acgatggtct gccaggcatc aatgcgcgga cggtcggcgc
     3661 ggtcgtaata cggctggaga cgcttgccag tcagcagctc cgtgttcggg atcaggaagt
     3721 tcagctccag ccgcccctta tcctggtgct caacccacag cacgctgtac tggtctttgt
     3781 cgagtccggg catcagaacc cgttcaaagc tcgccatcag cttttcgcgc tgcccgggcg
     3841 gtaaatcctt ttcggcaaag gacaggacgc cggatgtgta ctttttttgc gtagggtgag
     3901 gcgtcgataa gctccctgac ttcttccggc ttcccctgca gaacggtggc gccctcgcgc
     3961 tgtcggtctt tccccagcag ataatccacc ggaccggcgc caccgccgcg ccctcgcggg
     4021 tgaaacttaa cgatcatcgt ctgcaccctt ttccagcacg gcatgccgca gccgctcgag
     4081 tccggcatct atggccatca gcgcggcgac gacctgcacg cggtcgtgcc cgctgccacc
     4141 accggcgtta acctgccggg caatctggtt aaggttgttc cccatgccgg caagctgacg
     4201 aagcagcgcc ggcgagatcg agggaagctt gccggcgcgg gcgggtttct cgtccaggca
     4261 cgtctggcgc atccaggctg caagctgttt accgtcgcag cgctccagca gacgccggtg
     4321 ctcatcctcg gtcacccaca tcgtgagcat tttgctgcgt ttgtctgcca atccggcctc
     4381 ctgataacgc cgggacgggc gggaaatctt ttcccggtcg gcgcggtgtt ggacaagccg
     4441 caggcgcgtc agtggtttta gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc
     4501 tagcggagtg tatactggct taatatggta aatcgaagtg ggaattcagg gaagtgcttc
     4561 atgtggcagg taaaaaatgc gtctgtgggg cgtaagtgcg aaatgctggg gacgatgaaa
     4621 tgacgcctcc tcgctcactc ggtcgctacg ctcctgccgt gagactgcgg cgggcgttac
     4681 cggctcacga atggcgggat aaagagatgg cgctcaaatc aggaagggct gggaaaagga
     4741 tgcggcgttg ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatatgacg
     4801 ctcaaatcag tggcggcgaa acccgacagg actataaaga tcccaggcgt ttccccctgg
     4861 tagctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4921 atgtggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     4981 caagctggac tgtatgcacg acccccccgt tcagtccgac taccacgccc attccggtaa
     5041 ctatcaactt gagtccaacc cggaaagaca cgacaaaatc gccattggcg gtagccactg
     5101 gtaagcagga tgtgcgagag atgtatcgag agttcttgaa gtggtggccg gactacggct
     5161 acactggaag gacagatttg gtgacacgtc tcgcacaaga ctgttaccac ggttcagaag
     5221 ttccccaact tactgaacct tcgaaaaacc acctccccag gtggtttttt cgttttcaga
     5281 gcaagagatt acgcgcagag caaaacgatc tcaagaagat cattttacaa attggataat
     5341 atccctccgc ccctaacact aatataaaat tatcgggctt gcatgcccct caccattttc
     5401 cccatatcca ccaccaaaca atagttcgtc gagttctttg ctagcttgat caatacacgc
     5461 agccacatga atagcagaag ggtactcatc atactgaaaa ttatcgcttt tttcaggagg
     5521 gagtcgatca taatacggat gacaatccac ataataatga cgtattagca tcccagtttt
     5581 atcttcgtac tctgatggct gcaacgtact ccctgggaaa gcttttgcaa catgtatagc
     5641 agcttgaaag gtatcaaaat atcgcccaaa cattggcgtt tcaaagctag gcgactctgt
     5701 cgaaaaatcc cccataactt gcgccaaata accattatct atctgccatt gaacatctga
     5761 tcccatagcc cactctccca gagttactcc taataaataa ccataatatg tatttaccat
     5821 aacgcttctt ggccgcaccc cctgagagcc cctcagcgcg tttctgcggc ctgagggccg
     5881 tttccggcac ctttccccgg cccaaaccct gaaaaacgcc cctgataatt tctgcgcggc
     5941 tgcatggcta tgcacaagag tgaatggcgg ccggtttcgt gaaggaaata ccgtgaaagc
     6001 tccgaaagaa acgcttaatt gcacagcagc acatgaacgt gatcagatga aatctgtcta
     6061 ctgccgcact tcttcacgaa aggcccgctg agcgtcagat tcgggccttt cttactaaaa
     6121 agatttcaat agaatttttt agcaggcgat ttttatctat aaccatacat tgagcataga
     6181 agacccaaat ttatgtatga ctcaatagaa aaaaacaaat cacagccatc taaacactga
     6241 gacagcaagc caaacaaaaa tcaaagaaat aactccattt ttaatcaaat aatatagttt
     6301 tcgattcctt ttttttagct tctgacctgc atcacgaata gcattaacat atttaaataa
     6361 atgatttata gcaccagtcc tgtcattatt atcgttgggt gatgctgccg ctgacattag
     6421 caccccacct atccacctat ttattttttg tgaccaccgc cacttcatcg gcctttctat
     6481 atcacagaac aaaataatac gtgtctgctc ggtatcattc tgagcccagt gaacaaaagt
     6541 ttcgtcaaaa attatcactt caccatcatg ccaactgcgg cgtacaccat caacttcaat
     6601 aaaacaccgt tcatcatttg gtgttgataa tcctaaatga taacgaactg aaccggcata
     6661 agggtcacga tgctttccta aatggcttcc tgaaggcaat tcagcaaaca tcgctgcttt
     6721 aatacaggga atagagctaa ccagttgggt tgttactggg caaagccgct ccgctgatgg
     6781 atgagcatct ccataccact tgaggtaaaa gcgcttccaa ccgcgcttaa aaaatgtatt
     6841 aaatcctgca tcattatttt tttcagatga tttaatgtgc ccttgcaaat taacagcttc
     6901 gtctcttata acctgccagt tatcaattaa tggttgaagt tcaggaaatg tttctggggg
     6961 aaaccatgcc tgctttgacg gcagcttcga aaagcctgtc ataaacatat ttatcggagc
     7021 cataaatgtt gaatgatcaa ataactgcct ggaaaacttt tgttttattt taccgcgaga
     7081 atgcgcatac aatacactga taagaaaaac aaaaataata ataagtgaca gcatgtgtat
     7141 attccttgtt cacgccaata ataaaaatct cttaaagata acataatgaa tgcacttaaa
     7201 ttataatcta aatgctattt atcaatagta taatcgaagc ttcaccagaa catatctact
     7261 acacaggcag agaggatttc agcaggaatt atgctgaaat gaatcaaagg gacggaccgt
     7321 gatatacccg ctgtcgctgt gatcgttcga gctccagcgc tttctgatgc cgcgcctccc
     7381 gcacctgctg cagctcgacg gcattcagcc gctcgacaac cggcgcagct gatcgctcaa
     7441 actctgcacc tgcctgctca agcccgtgac gcgctcgctc agcgccgcgt tctcccgttg
     7501 cataagacca gacatcgtcc gccattccgc gaaggcgctc tcccactcgt ccagcctttt
     7561 cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctctgtcat
     7621 gtgctatttc cccgccagta tcatccagct cgaatcctct ccgaaccgtc ccgcttccgg
     7681 gttcaccctc acgcagcggc gtctctgctc gccgcaggtc gagagctgca cgccgctgtt
     7741 ccgctcggac agcatcgcct gcgtgctctt ctgctcccgg atgctcacgt aattgtcgag
     7801 tatctgctga ctctgccacc acagaatggc cgcgttcgag gcgatcagca gcacggagac
     7861 caggacgatg gtcagccacg tctggctgac catgcgcagc atccctttcg tacgctggct
     7921 catggctgag gacagcttcc ggtcgtggtc gaggatggcg gcgctgattc tcttctcgct
     7981 ttcgctcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt tcagcatctc
     8041 gccggtactc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca gcgttaaaag
     8101 actgttcata gatggctccc ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc
     8161 tgatgctgct ttttgtggtg cgtaccacct caaaacctgc gctttccagc gcctcagtga
     8221 cgtcctggcg ggttttcagc tccccggacg aggccaaggc cagtaaaccc cgtgtaattg
     8281 cctgagcggc ttcctgcttc gtttccggca gcgcggacgg cgtgaccagc gcccgccggt
     8341 tttcaggcgc gttcgggtca tgcagcccca gcctgccgtt cacgatggtc tgccaggcat
     8401 caatgcgcgg acggtcggcg cggtcgtaat acggctggag acgcttgcca gtcagcagct
     8461 ccgtgttcgg gatcaggaag ttcagctcca gccgcccctt atcctggtgc tcaacccaca
     8521 gcacgctgta ctggtctttg tcgagtccgg gcatcagaac ccgttcaaag ctcgccatca
     8581 gcttttcgcg ctgcccgggc ggtaaatcct tttcggcaaa ggacaggacg ccggatgtgt
     8641 acttttttgc gtagggtgag gcgtcgataa gctccctgac ttcttccggc ttcccctgca
     8701 gaacggtggc gccctcgcgc tgtcggtctt tccccagcag ataatccacc ggaccggcgc
     8761 caccgccgcg ccctcgcggg tgaaacttaa cgatcatcgt ctgcaccctt ttccagcacg
     8821 gcatgccgca gccgctcgag tccggcatct atggccatca gcgcggcgac gacctgcacg
     8881 cggtcgtgcc cgctgccacc accggcgtta acctgccggg caatctggtt aaggttgttc
     8941 cccatgccgg caagctgacg aagcagcgcc ggcgagatcg agggaagctt gccggcgcgg
     9001 gcgggtttct cgtccgagca cgtctggcgc atccgagggc tgcaagctgt ttaccgtcgc
     9061 agcgctccag cagacgccgg tgctcatcct cggtcaccca catcgtgagc attttgctgc
     9121 gtttgtctgc caatccggcc tcctgataac gccgggacgg gcgggaaatc ttttcccggt
     9181 cggcgcggtg ttggacaaag ccgcaggcgc gtcagtggtt ttagcgggtt tcggggcgca
     9241 gcccctggac cagtcactca gcgctagcgg agtgtatact ggcttaatat ggtaaatcga
     9301 agtgggaatt cagggaagtg cttcatgtgg caggtaaaaa aatgcgtctg tggggcgtaa
     9361 gtgcgaaatg ctggggacga tgaaatgacg cctcctcgct cactcggtcg ctacgctcct
     9421 gccgtgagac tgcggcgggc gttaccggct cacgaatggc gggataaaga gatggcgctc
     9481 aaatcaggaa gggctgggaa aaggatgcgg cgttgccgtt tt
//
