LOCUS       NZ_CP050528             5679 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Acinetobacter baumannii strain VB2139 plasmid pVB2139_2, complete
            sequence.
ACCESSION   NZ_CP050528
VERSION     NZ_CP050528.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14414747
            Assembly: GCF_012935165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., francis,L. and
            Kirubananthan,A.
  TITLE     Complete genome sequencing for A.baumanii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., francis,L. and
            Kirubananthan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116.6082566x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:23:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,868
            CDSs (total)                      :: 3,772
            Genes (coding)                    :: 3,692
            CDSs (with protein)               :: 3,692
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 19 of 80
            Pseudo Genes (incomplete)         :: 51 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 7 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050528.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VB2139"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pVB2139_2"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            complement(join(5504..5679,1..40))
                     /locus_tag="HBN30_RS19255"
                     /old_locus_tag="HBN30_19255"
     CDS             complement(join(5504..5679,1..40))
                     /locus_tag="HBN30_RS19255"
                     /old_locus_tag="HBN30_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002011639.1"
                     /translation="MQNELERYKNFDPTNAPLVKPSFVKKLQERHTIAQAQAFDADVVA
                     WLSSQDNETKQHINDMVRHIMALKRA"
                     /name="hypothetical protein"
     gene            complement(30..326)
                     /locus_tag="HBN30_RS19260"
                     /old_locus_tag="HBN30_19260"
     CDS             complement(30..326)
                     /locus_tag="HBN30_RS19260"
                     /old_locus_tag="HBN30_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002011632.1"
                     /translation="MPLIEYYGLLFEWHDQKMDLVYRGREITFEEVCSIFLDPALISFE
                     DVGHYDEQRLISVGLSNRGRLLTVVWVERGDVARIITAFEPSHHQKRRYSNAK"
                     /name="BrnT family toxin"
     gene            complement(367..867)
                     /locus_tag="HBN30_RS19265"
                     /old_locus_tag="HBN30_19265"
     CDS             complement(367..867)
                     /locus_tag="HBN30_RS19265"
                     /old_locus_tag="HBN30_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025464991.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQKVIAEQTH
                     STVSKSLNTGLQEGTAELIKTAKALGHLKNSIESTSDQFSWKIITIILGIFAILMLGII
                     WLFSVYIKPLENIPQIESLRQQGIQFDIGSCTVGEESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     gene            complement(864..1787)
                     /locus_tag="HBN30_RS19270"
                     /old_locus_tag="HBN30_19270"
     CDS             complement(864..1787)
                     /locus_tag="HBN30_RS19270"
                     /old_locus_tag="HBN30_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002011644.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQSDLEHSDYGN
                     MPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGSKY
                     PYQFAIHNPKAMDGNDQPHIHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDNTG
                     KSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKDPEKKLLPSQAKD
                     PEIREALQQSRTAYKELERLDLGDPKNDLKDLKNSPISDKEIKQGIESFKADFASFKQL
                     ALQQYKEQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            complement(1787..1942)
                     /locus_tag="HBN30_RS19275"
                     /old_locus_tag="HBN30_19275"
     CDS             complement(1787..1942)
                     /locus_tag="HBN30_RS19275"
                     /old_locus_tag="HBN30_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="WP_161772599.1"
                     /translation="MQLKQVLGKIKDISFGVIAAPLIIIIMMWASILDQRTFKKELDRK
                     KAQEKT"
                     /name="heme exporter protein CcmD"
     gene            2223..2639
                     /locus_tag="HBN30_RS19280"
                     /old_locus_tag="HBN30_19280"
     CDS             2223..2639
                     /locus_tag="HBN30_RS19280"
                     /old_locus_tag="HBN30_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_025464995.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     ILLKTAEIDPNDMKWQIWKELGQETLNHRKKDKK"
                     /name="conjugal transfer protein TraD"
     gene            2800..3297
                     /locus_tag="HBN30_RS19285"
                     /old_locus_tag="HBN30_19285"
     CDS             2800..3297
                     /locus_tag="HBN30_RS19285"
                     /old_locus_tag="HBN30_19285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031380830.1"
                     /translation="MATAIRADYCTNMTINNCSFSGFNNCIEMNFCTNFNILGSGFFNS
                     NNAVFLSNCYGKGAISNNYTHNVNNLISVADSKLKYPLPSKNKEKHKIIKKEQKKNSSI
                     RNIEFNKKIKELINLEANQKFIKRNHQLACKIKYIKANLGKLEAESLLFLTQALMQYDL
                     QS"
                     /name="hypothetical protein"
     gene            complement(3789..4568)
                     /locus_tag="HBN30_RS19290"
                     /old_locus_tag="HBN30_19290"
     CDS             complement(3789..4568)
                     /locus_tag="HBN30_RS19290"
                     /old_locus_tag="HBN30_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_169057934.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEALNLLNSVAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     ARG             complement(3793..4568)
                     /name="aph(3')-VI"
                     /gene="aph(3')-VI"
                     /gene_name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.84"
                     /coverage="100.0"
     ARG             complement(3793..4568)
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.84"
                     /coverage="100.0"
     Typing          complement(868..1787)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagggtcaaa atttttatag cgttctagtt cattttgcat tgctgtacct ccgcttttgg
       61 tgatgtgatg gttcaaatgc agtgattatt ctcgcaacat ctccacgttc aacccaaacc
      121 actgttaata gtctaccacg attgcttaaa ccgactgata ttagtcgttg ttcatcatag
      181 tgtcctacat cctcaaaaga gatcaatgca ggatcaagga aaatactaca gacctcctcg
      241 aacgtgattt ctcgccctcg atagactaaa tccatttttt ggtcatgcca ttcaaataac
      301 aagccgtaat attcaattaa tggcatgatt acctcttttc ctctcgtgaa ataaaaaata
      361 tgcctattat tttgggtcta tgacacaaaa atctccattt tttccatatc cgcactgctt
      421 tttcatcaca cgaacacacg gtttagattc ctctccaaca gtacagcttc caatatcaaa
      481 ctggatacct tgctgtctta agctctcgat ctgagggata ttttctaaag gtttgatata
      541 aacactgaat aaccaaatga tgccaagcat cagaatagcg aaaatgccta aaataatggt
      601 gatgattttc catgaaaatt gatcactagt tgattcaatc gagtttttta aatgtccaag
      661 tgccttagcg gtcttgataa gctctgctgt accctcctgt aagcccgtat ttagcgattt
      721 tgatacagtg ctatgggttt gctctgcgat gaccttctga atgtgttctt tcgtgatttg
      781 aagctcttta acggcctgat tgactaggtt ctgctgttgt tctgcaactg ccatcagtgc
      841 atataacttt ttttcatcag gtgtcatcgg ctcatccctc taaacttcat tgttttctgt
      901 tgctctcgtt ctaatttctg ctgttctttg tactgctgta aggcaagctg tttaaagctg
      961 gcaaaatcag ccttaaaact ctcaatgccc tgtttaattt ctttgtctga gatagggctg
     1021 tttttgaggt ctttcaggtc gtttttaggg tcgcctaaat ctagtcgctc aagctcttta
     1081 taggctgtac gtgactgttg caaggcttcc ctgatttcag ggtctttggc ttggcttggt
     1141 aagagtttct tttcagggtc tttctctatg ccttgctctt tgtagcttcg catatcaata
     1201 cggctgtcaa tttggtgttt ttccaaatgg ctattgcata aatccgccca cctttggcgt
     1261 aggtctttaa tatcagtttt tcgttcctgg taactcttgc ctgtattgtc ttttttagct
     1321 ccgccacgtt caggattctt gctgttatag cgtttgaaat attgctctgg atctcgttcg
     1381 attccatctt gcagacgttc attaaacatg aggtggatgt ggggctgatc gttgccgtcc
     1441 attgctttag ggttatgaat tgcaaactga tacggatatt ttgagccaat ttcagactga
     1501 ataaagtctt caaccagttc taggcgttgc tcagcattca tctctctagg cagggcaatt
     1561 tcatattctc gataggtact gccattttta cgctcgtaaa ggtcggctgc ttgccaaaag
     1621 gtaatcggat tatgttcagc ccatttcggc atatttccat agtcactatg ttcaaggtcg
     1681 gattgttcct gtttcagctt tttttcttcg tcccgatcaa tatattcggc atgcggtgct
     1741 gccttacctg ccttaccaac tttcacactt aaacgagcta tcgccattag gttttttcct
     1801 gtgccttttt tcggtctagt tctttcttaa atgttctttg atcgagtatc gaagcccaca
     1861 tcataatgat gataattagc ggtgctgcga tcacgccaaa actaatatct ttgatttttc
     1921 ccaatacctg ttttagttgc atgctgttgc tcctagcttt ggtacttctt tttttcattc
     1981 aattttcaat tgaacgaaaa aaagaagttt cagggtttta gggatgaagt ccctaacgtg
     2041 cttaggtttc tcaatgaaat tgagaaacgt ctaagcgcgc catcaaattt ttgatggaag
     2101 tacaggcgat aatctccaga acgactttaa caaaattacg ttactcgaac gtaaatattt
     2161 tgttatcgtt ctttgagtat tgagcctgtg cttttgcggt atatgaatta atggaattga
     2221 atatgaataa tgattggctg tctgaaaaaa tcacatatat ttctgcttta aagaatccta
     2281 gtgatgctca aaaactttta cttgaattag ccaaaattca gtacagaaca cccgaccaag
     2341 agaaaaaaat caatgccttg atcaaagctg aaaaagccat agatcgagca aataagcaaa
     2401 aagttgcagt tagaaaatta ttgaatgccg aaaaggaagc agaacgtaaa gctcgtacac
     2461 gtcatctcat tcaattaggc gcactatttg aaatagctaa cctagatcag cgtgacccag
     2521 ccgaattact tggcattctt ctaaaaactg ctgaaattga tcctaatgat atgaaatggc
     2581 aaatttggaa agagctagga caagaaactt taaatcatcg taaaaaagac aaaaagtgat
     2641 tgttcagaat ttttattcaa ctttatctaa gcacgtaaaa aactcatcta tatacagagg
     2701 gtctaaaaat tatatttttg atttataaag ataattaaag attactttgc ataaggtgta
     2761 ttatcttaat aataaggcaa ctaaggtagg atgaatttta tggcgactgc aattcgtgca
     2821 gattattgta caaatatgac tataaacaat tgctcttttt cagggtttaa caattgtata
     2881 gagatgaact tttgtactaa cttcaatata ttaggtagtg gtttttttaa ttctaataat
     2941 gctgtctttt tatctaattg ctacggaaaa ggagctatat caaacaacta tactcataat
     3001 gtaaataatt taatttcagt tgctgattca aaactaaaat accctcttcc ttcaaaaaat
     3061 aaggaaaagc ataaaatcat aaaaaaagag cagaagaaaa atagttcaat aaggaatata
     3121 gaatttaata aaaaaattaa agaacttatt aacttagaag ctaaccaaaa atttattaag
     3181 agaaatcatc agttagcttg taaaataaaa tatataaaag caaatcttgg aaaattagaa
     3241 gctgaaagcc ttcttttctt gactcaagca ttaatgcagt acgatcttca aagttaagat
     3301 gatggtatga caattttata tactccataa accctttaaa ttaattaggt ggtttatgtc
     3361 gcacttcaag ttttactctg ccaagtattg atattattaa agctcttaag ataatttaac
     3421 attcgtcatt tcccataaga atttaataaa atactatatg gctgacagat agataaaaga
     3481 ctaagaaaag aaaagcagac aaaaaaatac catcgataat aaaattggtt aaaaatgttg
     3541 cacgatgaaa tttaaataaa cttgcaaagc taataaatta acacttttat attcaaaaaa
     3601 acttaaatca caagttaagc taataaacag ttttagctat tttaaataag ctcattttaa
     3661 aaaccaaaat ttagatttat tggtgatata aaactttaaa tcaatagctc cactaacgct
     3721 cttcacttat ttatttaaaa tttagcgacg taaaaagaca attaagtatt tttagataat
     3781 gcttggaatc aattcaattc atcaagtttt aaaaaataat tccttttgtc aggtctatca
     3841 ttttttaaat gctttaaaaa tattttagca gtttcctccg atgcatcctc tcttaggcaa
     3901 cgttcaacaa aggatatatc tacaaattca tctgctaacc cagcacggcc aagatctaaa
     3961 aaataaattt cattgaattt atctataaaa atattactat ccgtgatatc gccatgagaa
     4021 aaaaccaatc tttcttcaac acgagtctca tttaactcat tccatagact taggtaagtt
     4081 ttatggtctc cccataattc agcgtcaaaa tcatcttgat ctatatcgtc aaggagttgg
     4141 ttatcaataa aaaattttga ctcttttaac cgatgatcaa tgtttgaaat aaatggacaa
     4201 tcaataatag caactgaatt taacagattg agtgcctcct tatagatagc aagcaattct
     4261 tggtctgtta aaaaaagcgc tgaaattggt tttgcattga tcgctttagt gatcatgaat
     4321 tcaaactgct catcctgaaa agtcatgatg agttcaggca cctttaattt ctcagagagc
     4381 caactcaaca ttttcgcttc gcgagagaca ctgtatgtgg tctctgtata taaagtgcta
     4441 gatcgcttaa gaaaaaaagt ttcattattt cgattaaaag aataaacatc cgatggcgac
     4501 tgaccaattt tatttggctc taaaacactg tttccaataa attgttgaat aatattgggc
     4561 aattccatca agttttcctt ttattcagca ttaaaacccc gcaaatgcaa ggcctagtaa
     4621 atagatgatc ttaatttggt tcactgtagc aaaaatatgg ggcgaattca aaactggttc
     4681 aatagcctaa gcagaaaaag gagtatttga ttatccgcac tactatactt cctaaaaaaa
     4741 aatgctctca acttctcaac tcgtacccaa agcgtcgcac ttcgtgcttt ggctttggaa
     4801 acggttgaga gagtttcggc aatactctcc ctaaaggcgg tcatttaaaa aaatgacgca
     4861 tcctttaagt attgccgatt taccgcatat tcttttggct tcggagccac gtatatcggt
     4921 cgtatccgca catagctttg caaactatgc agacaaggac taaaggtctt gcacctctcc
     4981 ccacacgctc cctgtcgaag tatggcggta tctctgcgag ggtttcccca acatacaact
     5041 taaccaacct atacaactac tcttaacgct ccgcttttgt gagattcccc tgtagcggtt
     5101 tagcctttcg actatcgcta cgttgcacca gtgacactta ttccctccac atgcaacgca
     5161 tgccaatccg taaaacggac acgcaggagc aggcaaagct cgatctagga tgcttatttg
     5221 atgatttaaa acacggatta acgaagactt atcattttga gtcttgatct aaacactgtt
     5281 tattgtgaaa atagagttca ggtacaagcc tttgtgtgaa atctgcacga gttggtagct
     5341 ctgctgatct tcgttacttg atgttccacc atcaaataac tgcgtaccgc tgaatataaa
     5401 gcgaaagccc attgagttgc aagattcaat gggctttttc tttgatcaat cagatattaa
     5461 gacaaaaaag agaggtcata tctaatcatg actaaatccc taatcaagca cgtttcaatg
     5521 ccataatatg gcgaaccata tcgttaatat gttgttttgt ttcattatct tgtgaactta
     5581 accatgcaac tacatcagca tcaaaagcct gtgcttgagc tatggtatgg cgttcctgta
     5641 atttcttcac aaagctaggt ttaaccaatg gcgcattgg
//
