LOCUS       NZ_CP050435             9847 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain PM194229 plasmid pPM194229_3,
            complete sequence.
ACCESSION   NZ_CP050435
VERSION     NZ_CP050435.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14422682
            Assembly: GCF_012935125.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Mathur,P., Amala,A., Francis,L.
            and Kirubananthan,A.
  TITLE     Complete genome sequencing for A.baumanii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Mathur,P., Amala,A., Francis,L.
            and Kirubananthan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 185.349194x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:56:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,022
            CDSs (total)                      :: 3,927
            Genes (coding)                    :: 3,798
            CDSs (with protein)               :: 3,798
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 40 of 129
            Pseudo Genes (incomplete)         :: 85 of 129
            Pseudo Genes (internal stop)      :: 19 of 129
            Pseudo Genes (multiple problems)  :: 12 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9847
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="PM194229"
                     /isolation_source="BAL"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pPM194229_3"
                     /geo_loc_name="India"
                     /lat_lon="28.5659 N 77.2111 E"
                     /collection_date="2019"
                     /collected_by="AIIMS Jai Prakash Narayan Apex Trauma
                     Center"
     gene            1..924
                     /gene="repM"
                     /locus_tag="HB663_RS20090"
                     /old_locus_tag="HB663_20090"
     CDS             1..924
                     /gene="repM"
                     /locus_tag="HB663_RS20090"
                     /old_locus_tag="HB663_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_000845851.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNIINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITLSDFRQKIGVLDTEYKRMYDFKKYVLDIALKQVNEHTDITVKVEQHKTGRSITGF
                     SFSFKQKKSVTKSAKSIGVSEDITITLTDAQRYSFASKLSELPEMGKLSQGTESYEQFA
                     VRIADMLKQPEKLKELTPLLRKVGFQ"
                     /name="repM"
     gene            992..1537
                     /locus_tag="HB663_RS20095"
                     /old_locus_tag="HB663_20095"
     CDS             992..1537
                     /locus_tag="HB663_RS20095"
                     /old_locus_tag="HB663_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000736775.1"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFAEAIRV
                     FGEPQKKDTNVNQSQSINSQNLTEVDLLKRQVDILEKQLNQALFRENQSLERESFYQDQ
                     IEAMQRLLEAPKANMTTFTDQEQSQANVAQVQPEPNIDDPKHDGLTTPENKRIPVPEHV
                     EPEPKKRGFWSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1744..4416)
                     /locus_tag="HB663_RS20100"
                     /old_locus_tag="HB663_20100"
     CDS             complement(1744..4416)
                     /locus_tag="HB663_RS20100"
                     /old_locus_tag="HB663_20100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_148307306.1"
                     /translation="MNILQRKSSMVLYAFEQSIGDFVLNYKDLNIGSRLIEDITEREIS
                     KGRSFDPNQIKDVVEATYLGELFQIAIDLTSNDSSHDLVKYLYSLFHDFEIHLVRNAIS
                     HPNRQFLDFFWYRLAAVATDPVIDILGLDKVKKAFISANEGILDDPPDDWLNKVVWEIP
                     NNLPSTFEHAVTGLIGRNKERKQLETYMSQPRVSTVALVAPGGVGKTALALDLLHYIVH
                     RPSAVNRLDGVVFVTLKTEKLTAQGIQQLTAIQTIAEVKNKIVESINDIFDEDLKNFED
                     VASLYDQKRILLCIDNLETVLREEQILFDDFNNTLPGGWKVLITSRITVENTKTLSLHP
                     LDQKSGEILARKYQESRGISTLENHDYEKISKACFFNPLAIRLTIDLINLGRDIPTSIS
                     EAQKNIAEFSYNNLIEALKPTSIQILEAIFVENVSTRASLCHVLEKDLDEISDSVGELS
                     RTSLIVKASQKDIETYSLNDSIKDLLLLDPKNLEVRNKVQNKLLEQKNQVREIDFHQEK
                     RQYPIWHRDNIPLDTPEGLKILVKNVNMAVRRKGETSTLSELIRKLRDAENIYKTYAIY
                     YKSLAKLLDKLSDSHGQKDALLKGLAIDDTNPNILYMLARYYADQGDYEASTKQYQKLI
                     ELGWIQDNEENIEFSRTIYNGYFLNLLYSKEYQRILDETKKWKEDENFSPLLGVYRATA
                     WKRTIELYGAEESDKIESAINSSLAIFDEVFRKYGYSVEACKQATKVFEEIIFYANNPL
                     KSDEKLKIFLEKTKKNITEISNTYLDYDWTNLYKKLSSIQISDNPFYNLNRSGYKSPSQ
                     MYADGSKEGDFIFVKIETIRNNYLFSSDKNQTQYFVHSNQYRGDVNWYKLKEGELLKVH
                     PLPPDSSQSAIKVKAAFSC"
                     /name="ATP-binding protein"
     gene            4556..5023
                     /locus_tag="HB663_RS20105"
                     /old_locus_tag="HB663_20105"
     CDS             4556..5023
                     /locus_tag="HB663_RS20105"
                     /old_locus_tag="HB663_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004727917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="WP_079393076.1"
                     /translation="MIEKGAIISDCNKYRYSLWRIWDKEKPIFTFVGLNPSTADHQQDD
                     RTIRRCINFAKSWGGGGIYMANLFAYRATDPQEMMSQEDPIGPDNDAHLKQLAAESHKL
                     IACWGNDGVFKARSTFVKQLLTDKLYYLKLNKSGEPGHPLYIHSSTQIQPF"
                     /name="DUF1643 domain-containing protein"
     gene            5562..5918
                     /locus_tag="HB663_RS20110"
                     /old_locus_tag="HB663_20110"
     CDS             5562..5918
                     /locus_tag="HB663_RS20110"
                     /old_locus_tag="HB663_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5911..6213
                     /locus_tag="HB663_RS20115"
                     /old_locus_tag="HB663_20115"
     CDS             5911..6213
                     /locus_tag="HB663_RS20115"
                     /old_locus_tag="HB663_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            6206..6415
                     /locus_tag="HB663_RS20120"
                     /old_locus_tag="HB663_20120"
     CDS             6206..6415
                     /locus_tag="HB663_RS20120"
                     /old_locus_tag="HB663_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069475.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWLKYYEIA"
                     /name="hypothetical protein"
     gene            6590..7108
                     /locus_tag="HB663_RS20125"
                     /old_locus_tag="HB663_20125"
     CDS             6590..7108
                     /locus_tag="HB663_RS20125"
                     /old_locus_tag="HB663_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447195.1"
                     /translation="METFAEPVFSNDLLAKAESGDTSAQLELAEIYLYGHGVDSDENQA
                     EIWAIKSAENGNVAAMFWLADGYVTYARLIEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNIKKALELREKAAKLGNKKAMRSLSAMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            7147..7659
                     /locus_tag="HB663_RS20130"
                     /old_locus_tag="HB663_20130"
     CDS             7147..7659
                     /locus_tag="HB663_RS20130"
                     /old_locus_tag="HB663_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="WP_001089574.1"
                     /translation="MNSVHEIIEKIHNEWEIEPKKAIQRGRECPFPLQCSLNLKSKIYP
                     QIPQVLLPKVLEDFYTVSNGADLFKDQEYGQWGLKLYSIEEVIFASKIYKSNRNNDALQ
                     SDLIIGEFYGDSDLLLVRCDPNSDDYGSIFVVLPIDQRQDWYIVANTFQEFISKFYETQ
                     GDKFWEP"
                     /name="SMI1/KNR4 family protein"
     gene            complement(7766..8083)
                     /locus_tag="HB663_RS20135"
                     /old_locus_tag="HB663_20135"
     CDS             complement(7766..8083)
                     /locus_tag="HB663_RS20135"
                     /old_locus_tag="HB663_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            complement(8288..8479)
                     /locus_tag="HB663_RS20140"
                     /old_locus_tag="HB663_20140"
     CDS             complement(8288..8479)
                     /locus_tag="HB663_RS20140"
                     /old_locus_tag="HB663_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102616.1"
                     /translation="MSTELVNSANIISFPKPCAFCESTEHVQLFAGLMLCRSCQENIKI
                     TNPDLFATNDQIQQKAQD"
                     /name="hypothetical protein"
     Typing          2..605
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.835"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaacag aactaatagt taaagataat gcattaatta atgccagtta taaccttgat
       61 ctagtcgagc aacggttaat tcttctagct atcgttgaag caagggaatc gggtaaggga
      121 ataaatgcta atgatccatt aacagttcat gctgaaagct acatcaatca atttggtgta
      181 catcgaaaca cggcttatca ggcattaaaa gatgcttgtg atgatctatt tgcaagacaa
      241 ttcagttatc aaagtcttag tgaaaaaggc aacattatta atcacaagtc aagatgggtg
      301 agtgaagtcg cttatattga taatgaagcg gttgttagac ttatttttgc tcccgctatc
      361 gtgcctttaa ttacaagact agaagaacaa tttacaaagt atgaaataca acaaataagc
      421 aatttaacaa gtgcttatgc cgttcggtta tatgagatat tgattgcatg gcgtagtaca
      481 ggaaaaacgc ctcttataac tttgtctgat ttcagacaaa aaataggtgt actcgatact
      541 gaatacaaac gaatgtatga ctttaaaaaa tatgtcttag acattgcatt aaagcaagta
      601 aatgaacata ccgacattac tgttaaagtt gaacagcata agactggtag atcaattaca
      661 ggtttttcat ttagctttaa acaaaaaaaa tcagtgacga agtcagctaa aagtataggt
      721 gtaagcgaag atataacgat cactttaaca gatgcacaac gctattcatt tgcgagtaaa
      781 ttgtcagagc ttccagaaat gggaaaactt tcacaaggca ccgaaagcta tgaacaattt
      841 gctgtacgga ttgcagatat gctaaaacag ccggagaaat taaaagaact tactccatta
      901 cttcgaaaag ttggctttca ataaatgatg tcaattgaca ttgtaaagtt gacagtcaag
      961 tattaacaca aattgaaaac tggtttacat aatgaaaaaa ctgtcagttt cagagttggc
     1021 taagctttac ggatattcac gacaagccat atatgcccat ataaacaaag gaaatttatc
     1081 taaaggatct gatggattaa ttgacttcgc agaagccata agagtttttg gggaaccaca
     1141 aaagaaagat actaatgtca atcaaagtca atcaattaac agtcaaaact tgacagaagt
     1201 tgacctatta aaacgtcaag ttgacatact ggaaaaacag ttaaatcaag cactgtttag
     1261 agaaaatcaa tcactagaac gtgaatcgtt ttatcaagat cagatcgagg ccatgcagcg
     1321 cttactagaa gcaccaaaag ccaatatgac tacgtttacc gatcaagaac agtcgcaagc
     1381 gaatgtagct caagtacagc cagaacctaa tattgatgat cctaaacatg acggattgac
     1441 tactccagag aataagcgca ttcctgttcc agagcatgtt gaaccagagc ctaaaaagcg
     1501 tggcttctgg agtcgttttt ttcttccata tggttaactt aggctaaata aaaccgtgga
     1561 acattgctca ataaataagc gtggaacgtg aaaaatcata aaaaaaaccc accttgggga
     1621 agatgggcta taaactagaa actacagcat tgatttctaa ggaaaattat aacacatttc
     1681 gtataaggtg tattatgtta atttaagaaa atataactaa actttctaag tttttgactc
     1741 aatctagcaa ctaaaagctg ctttgacctt tatcgctgat tgactagaat ctggtggtag
     1801 aggatgaact tttaacaact ctccttcttt taacttgtac caatttacat ctcctctata
     1861 ttgattagaa tgcacaaaat attgtgtttg atttttatct gaagaaaata gatagttatt
     1921 tctaatagtt tctattttta caaatatgaa atcaccctct tttgaaccat cagcatacat
     1981 ttgacttggc gacttatacc cagatcggtt aaggttataa aaaggattgt ctgaaatctg
     2041 aatagaactt agtttcttat ataagttcgt ccaatcataa tccaagtatg tatttgaaat
     2101 ttctgtgata ttctttttgg ttttttcaag aaaaattttt aatttttcat ctgatttaag
     2161 ggggttgttt gcataaaata tgatttcttc gaatacttta gtagcttgct tacatgcttc
     2221 tacactgtaa ccatactttc gaaatacttc atcaaaaata gctaatgaac tattaatagc
     2281 tgattcaatt ttatcacttt cttcagctcc atataattca atagttcttt tccaagcagt
     2341 agctcgatag acccctaata aaggggagaa attctcatct tctttccact ttttagtttc
     2401 atctagaatt cgttgatatt ctttcgagta aagtaaattt aggaaataac cattatatat
     2461 ggttctagaa aattctatat tttcctcatt atcttgaatc catcctagtt ctattaactt
     2521 ttgatactgc ttagttgaag cttcataatc tccctgatct gcataatatc tcgctaacat
     2581 atacaaaata tttggattag tatcatctat agccaaacct tttaaaagtg catctttttg
     2641 accatggcta tcagaaagtt tgtctaataa tttagctaaa gatttatagt aaattgcgta
     2701 tgttttataa atattttcag catctcttaa ctttctaatt agttcagata aggtacttgt
     2761 ttctccctta cgacgcactg ccatatttac atttttaact agaattttaa gtccttctgg
     2821 agtgtctaaa ggaatattat ctctatgcca aatagggtat tgacgtttct cttgatgaaa
     2881 atcaatttca cgaacttgat ttttttgctc tagtaatttg ttctgaactt tatttctaac
     2941 ttctaaattt tttggatcta ataaaagtag atctttgatc gaatcattta agctataagt
     3001 ttcgatatct ttttgagatg ctttaactat taaacttgtc ctagatagct caccaacact
     3061 atcagagatc tcatctaaat ctttttcgag aacatgacat aagctagccc ttgttgatac
     3121 attttctaca aaaattgctt ctaaaatctg tattgatgtt ggttttaagg cttctattaa
     3181 attattgtaa gaaaactcag caatattttt ttgtgcctca ctaatagaag tgggaatatc
     3241 gcgtcctaaa ttaattaaat cgatggttaa acgtatagct aaaggattga agaaacaagc
     3301 ctttgatatt ttttcataat catgattttc tagagtacta atacctcggg actcttgata
     3361 ttttctagct aaaatttccc cacttttttg atcaagagga tgcaaactta aagttttagt
     3421 attctcaact gttattcgac tagtgatgag aaccttccat ccacctggta gtgtattatt
     3481 aaaatcatca aaaagaattt gttcttctct caatactgtt tctaaattat caatacatag
     3541 caggattctt ttttgatcat ataacgaggc cacatcctca aaatttttta aatcttcatc
     3601 aaaaatatca tttatagatt ccactatctt gtttttaact tctgcgatag tttgaatagc
     3661 tgtaagttgt tgaatacctt gagctgtcaa tttctcagtt tttaaagtaa caaaaacaac
     3721 tccatccaaa cgatttactg cactaggtct atggacaata taatgtaaaa gatctaaagc
     3781 taaggctgtt tttcctactc ctcctggggc gactagagca acagtagaaa ctcggggttg
     3841 agacatataa gtttctaatt gctttctttc tttatttcta ccaattaatc ctgttacagc
     3901 atgttcaaaa gtacttggta aattatttgg aatttcccag actactttat taagccagtc
     3961 atcaggagga tcatccaaaa taccttcatt tgcagaaata aaagctttct taactttgtc
     4021 taatccaaga atatctatta caggatcagt tgctactgca gctaatctgt accaaaaaaa
     4081 atctaaaaac tgtcgattcg gatgactaat cgcatttctg actaaatgga tttcaaaatc
     4141 atgaaataat gaatataaat acttgactaa atcatgagaa gaatcattac tagtcaagtc
     4201 tatagctatt tgaaataact cacctaagta ggtcgcctca acaacatctt taatttgatt
     4261 cggatcaaaa gatcgacctt ttgatatttc ccgttctgta atgtcttcaa tcaaacgact
     4321 accaatattt aagtctttat aatttaaaac aaaatctcca attgattgct caaaagcata
     4381 taagaccatg gaggatttac gttgtagaat attcatattt aaattttcta aggaattaag
     4441 tctcaactaa tcgatataac attttttaat ttaaaatcaa atagtaacta atcattattt
     4501 aaattttatt taacataaaa cctcttattc gaaatagatc aagaaggaca atgtagtgat
     4561 agaaaaaggg gcaatcattt ccgactgtaa caaatatcgt tattcacttt ggcgtatttg
     4621 ggataaagaa aaaccaatat ttacctttgt cggcttaaat ccatcaactg cagaccatca
     4681 gcaagatgac cgaaccataa gacgatgcat taattttgct aaaagttggg gaggtggtgg
     4741 catttatatg gctaatctat ttgcatatag agctactgat ccacaagaaa tgatgtccca
     4801 agaagaccct attggtcctg ataacgatgc ccacttaaag caattagcag cagaatccca
     4861 caaacttatt gcttgttggg gaaatgatgg tgtttttaaa gcgagatcaa cttttgtaaa
     4921 acaattgctt acggacaaac tctattatct taaattaaat aaaagtggag aacctggaca
     4981 tccgttatat atacattctt ccactcagat acagcctttt tagatcccat ttaacataat
     5041 gtgcgttatc cgaaatggcc ctttaagttt actttaagat caacaactta cattgagtta
     5101 aacccctgga acgatctcgt tcaaccgatt tagaaataaa tcggtttttt tatgttcaat
     5161 tctgatactt cttgccaata aaattggtta aaaaatgatc aaaaatacca attccataga
     5221 gtttgtaagc cttttttacc caaaagaatc cacaatttat gcggataacc attgggataa
     5281 attttgatta ttttttgtac gttttggctt acagcgtttc acgaaattgt ctgtatctat
     5341 cccccgatga agctcttatg atcgatctat atggacagga tgttcaatga cttaaaacaa
     5401 caataataat cagtcaggca ccatgaccat caggtagtca gccctaccta tgcgctccat
     5461 accgaaaaat cccgacatga cgtcgggatt ttttattgcc atttgcaaag tcattcaatc
     5521 agatttaata tatagactgt aatctatatt gtgattgtaa tatgtggaca gtcattacaa
     5581 cggatctgtt taatgaatgg cttgaacagc aagatgaagc aacacaagag aaagtcttag
     5641 ctgcattggt tgttctacaa cagcaagggc ctagtttagg tcgtccttta gtagataccg
     5701 tctacgattc taaatttacc aatatgaagg aactacgtgt tcagcatcgt ggtaaaccgt
     5761 taagggcatt ttttgcattc gatcccctaa gacaagcgat tgtgctgtgt attggtgata
     5821 agagtaataa aaagcgtttt tatacagaga tgctagcaat tgcagatgag caatacgcac
     5881 ttcatctctc aactttagga gatcaatcta atggctaaga gcttacaaga attattggct
     5941 agtcgctctc cagagagtca agctcgtatt caaaaaatgg ctgatgaatt acttttagaa
     6001 agtcagcttc accttatccg agaagagtta gaaatttccc agaaagaact agctgcaacc
     6061 cttggaatta aacagccgtc attatcagca atcgaaaatc gtggtcatga tcttaaaatt
     6121 tcaacgatga aaaagtatgt tgaagcaatg ggtggaaaac ttcgtattga tgtagagctt
     6181 ccaacaggaa aacacatagg gttcaatgtc taataataaa cctttgaaaa atagtactaa
     6241 attacttgtt aacttagaca aatttatatt tttagtcaat gctgccgata gcctagaaga
     6301 aattgaaatt attagagatt tatgctgtga atatttttca cattgtaaga ggccaagcta
     6361 ttacattgat atttttgaca atgcttattg gttaaaatat tacgaaatag cttaatcctt
     6421 ctagattcgc ataagagttt ctatgttaaa taggactaaa aatatactct aattttagag
     6481 ttttctttta ggtatgatgt aaaaacatac aagcctaaga gtttaattta aaggttaaga
     6541 attgaataaa ttagtaaata tttttatggc tttaggagtt tgcctttcaa tggaaacatt
     6601 tgcagaacct gttttttcta atgatttatt agcgaaagca gaaagtggag atacttctgc
     6661 ccagttagaa ttagctgaga tttatctata tggtcatggt gttgattcag atgaaaatca
     6721 agctgaaatt tgggctatta aatcagctga aaatggaaat gtagcagcaa tgttttggtt
     6781 agctgatgga tatgttacct atgctagatt aatagaggat gatgacaaaa acgattcttt
     6841 agaacatttc caaaaagctt ttaagtggtt tcagaaagct tcagaaaatg gtcattctga
     6901 atcaatggtt gagttagctg acctatatac tcgcgcagat agcggaatag aagttaatat
     6961 taagaaagct ttagaacttc gtgaaaaggc tgcaaagtta ggtaataaga aagcaatgcg
     7021 aagtctttcc gctatgtatc gtgatggtat aggcattcct aaaaatactg atttagctca
     7081 aagttggtgg gataagtctg aaaattaatt ttagtaaaag ttaaggattt tttaagagtc
     7141 taataaatga atagtgttca tgaaattata gaaaaaatac ataatgaatg ggagattgaa
     7201 cctaagaaag ctattcagag aggtagggaa tgtccctttc ctttgcagtg ttcattaaac
     7261 ctaaaaagca aaatttaccc acaaataccc caagtacttt taccaaaagt attagaggat
     7321 ttttatacag tatctaatgg tgcagatcta tttaaagatc aagaatacgg acaatggggc
     7381 ttaaaattat attctattga ggaagtaatt tttgcttcaa aaatttataa aagtaatcgt
     7441 aataatgacg ctcttcaaag tgacttaatt attggagaat tctatggaga ttcagactta
     7501 ttgttagttc gatgtgatcc taatagtgat gattacggct ctatttttgt tgtcttacct
     7561 atagatcaaa gacaagattg gtacattgtt gctaatactt ttcaagaatt cattagtaaa
     7621 ttttatgaaa ctcagggtga taaattctgg gaaccttaaa attatatggc cgatataagc
     7681 tctatttaac ttatgttcac ctaaaattta tttaattatc acaaaaatag tatcattttt
     7741 tgaatttcat ataacgcaca ttatgttaaa tggaatgttt atgagaaatt aatagtctat
     7801 ttttgtttga gattgaagta tatttaaaaa agttaagttg tttttttaaa tgcataactt
     7861 catcttttag ttttttgatt tgttgctctc tttcgtcctg tatagggtaa gtaagttcac
     7921 caaattttct aactaactga ctgtattcaa tcaatttatt gtagtcaagt tcgaaatctc
     7981 cgctttttat atatttaagt atagtcgagc gaggtttttt atacgctata gcagcatcac
     8041 tgatcgaaaa tttttttaaa aaatgatgat ttttacctag catactattt cacttaaggc
     8101 attattaatt tcgtatatat atgttaataa aattaacaca tacaagatat aaatctttaa
     8161 tcattattgg ttaataatcg aacgtttact ttacacatgc cctactctga taatttttct
     8221 attattaaat caatatgctt ttaaaaattt atattattaa cataatacac cttatacgaa
     8281 gcccaggtta atcctgggct ttttgttgaa tctgatcatt ggtagcaaac agatcaggat
     8341 tggtaatttt gatgttttcc tgacaactcc tgcaaagcat cagcccagca aaaagttgta
     8401 catgttccgt tgattcacag aaggcacatg gcttaggaaa agagatgata ttggctgaat
     8461 tgactaattc tgttgacata agaagtaacc ttggattgta catatttatt tttaataaat
     8521 ttcaatactt tatacctaat ttagccacta aaatttgtac attattgtat aatccatgtg
     8581 tacatattat tttttataat tttcattcac ttagacccaa aatagtattt atttttgtac
     8641 aacaccatgt acagaacaat aatcatataa atcaaatttt tagcataaaa aagtccatta
     8701 attttgtaca caattctgaa acttaaaaat ctaaactttc atcaattttt ttcataattt
     8761 caataaatta accttaattt taagatatat tctgaaattt ggtttgaaag ttgtttttac
     8821 attatatttc aatactttaa atcaaaaaat tggatttttt ttgaaaaact caatgaaagt
     8881 ttatttttta tttaaaaaca actagttata ttagttttta tccatttttt tgaaacagtt
     8941 tctatatgat aaaaaaacct ataaaaacca tatctagcaa aggtttgagg gttatcatct
     9001 ttagatgcgt ggtgtgtgac aaaaaaatcc cggcatgtgc cggattctgg attagaaaat
     9061 tggctaaagt gacgtaggac tggtgcttgg ttttacatgg aaaaaagtat ttattttctg
     9121 gtttataaaa acgtaaaaag atcagttttt gttcattcat ccaggttaaa aatttcaacc
     9181 taaaacttta attatgaaaa gcttcacaga aagcattcaa atgcgattta agagccttta
     9241 tctaaaaaac atagatctta tagcgaaaaa cagaaaacag ctcaaaaaac gcaaaataga
     9301 gtgaagtaaa gagatgtttt gactttagat agctgcatag agcgagtgtc tacgagcgaa
     9361 ctatcaaaat ttgcgtctag actctctgaa aaacattttt ttgccctctt tagcctaaga
     9421 aagcttaatt ttcatgcaga aatttgctcc tggaccgagc gtagcgagaa aaaaagctca
     9481 tgagcgaagc gaattccgag ttgcttttgc tttttcttaa agtcacgcaa gtattaacca
     9541 aaaaattgcc ccgacgaact gagcgaaagc gaagttcaat agagtttgag cgaagcgaaa
     9601 accaagggca atttttcatg acctgagctt ttaaatattt ttaaatcttt taaatgcttt
     9661 tagacatgct gaaagccttt atttataaaa ttcttagaga ctataaaacg aggtttacct
     9721 tgcattaaaa cgaggtttac cttgcattaa aacgaggttt accttgcatt aaaacgaggt
     9781 ttaccttgca ttaagcgagt ttatgatata gcctcgtctt attgaattaa aaatataacc
     9841 ttttcat
//
