LOCUS       NZ_CP050423             5679 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter baumannii strain VB2200 plasmid pVB2200_2, complete
            sequence.
ACCESSION   NZ_CP050423
VERSION     NZ_CP050423.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14415334
            Assembly: GCF_012935025.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., Francis,L. and
            Kirubananthan,A.
  TITLE     Complete genome sequencing for A.baumanii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., Francis,L. and
            Kirubananthan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116.2229039x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:21:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,869
            CDSs (total)                      :: 3,773
            Genes (coding)                    :: 3,693
            CDSs (with protein)               :: 3,693
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 19 of 80
            Pseudo Genes (incomplete)         :: 51 of 80
            Pseudo Genes (internal stop)      :: 17 of 80
            Pseudo Genes (multiple problems)  :: 7 of 80
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050423.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VB2200"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pVB2200_2"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            complement(740..955)
                     /locus_tag="HBN41_RS19255"
                     /old_locus_tag="HBN41_19255"
     CDS             complement(740..955)
                     /locus_tag="HBN41_RS19255"
                     /old_locus_tag="HBN41_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002011639.1"
                     /translation="MQNELERYKNFDPTNAPLVKPSFVKKLQERHTIAQAQAFDADVVA
                     WLSSQDNETKQHINDMVRHIMALKRA"
                     /name="hypothetical protein"
     gene            complement(945..1241)
                     /locus_tag="HBN41_RS19260"
                     /old_locus_tag="HBN41_19260"
     CDS             complement(945..1241)
                     /locus_tag="HBN41_RS19260"
                     /old_locus_tag="HBN41_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002011632.1"
                     /translation="MPLIEYYGLLFEWHDQKMDLVYRGREITFEEVCSIFLDPALISFE
                     DVGHYDEQRLISVGLSNRGRLLTVVWVERGDVARIITAFEPSHHQKRRYSNAK"
                     /name="BrnT family toxin"
     gene            complement(1282..1782)
                     /locus_tag="HBN41_RS19265"
                     /old_locus_tag="HBN41_19265"
     CDS             complement(1282..1782)
                     /locus_tag="HBN41_RS19265"
                     /old_locus_tag="HBN41_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025464991.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQKVIAEQTH
                     STVSKSLNTGLQEGTAELIKTAKALGHLKNSIESTSDQFSWKIITIILGIFAILMLGII
                     WLFSVYIKPLENIPQIESLRQQGIQFDIGSCTVGEESKPCVRVMKKQCGYGKNGDFCVI
                     DPK"
                     /name="hypothetical protein"
     gene            complement(1779..2702)
                     /locus_tag="HBN41_RS19270"
                     /old_locus_tag="HBN41_19270"
     CDS             complement(1779..2702)
                     /locus_tag="HBN41_RS19270"
                     /old_locus_tag="HBN41_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002011644.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQSDLEHSDYGN
                     MPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNAEQRLELVEDFIQSEIGSKY
                     PYQFAIHNPKAMDGNDQPHIHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDNTG
                     KSYQERKTDIKDLRQRWADLCNSHLEKHQIDSRIDMRSYKEQGIEKDPEKKLLPSQAKD
                     PEIREALQQSRTAYKELERLDLGDPKNDLKDLKNSPISDKEIKQGIESFKADFASFKQL
                     ALQQYKEQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            complement(2702..2857)
                     /locus_tag="HBN41_RS19275"
                     /old_locus_tag="HBN41_19275"
     CDS             complement(2702..2857)
                     /locus_tag="HBN41_RS19275"
                     /old_locus_tag="HBN41_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="WP_161772599.1"
                     /translation="MQLKQVLGKIKDISFGVIAAPLIIIIMMWASILDQRTFKKELDRK
                     KAQEKT"
                     /name="heme exporter protein CcmD"
     gene            3138..3554
                     /locus_tag="HBN41_RS19280"
                     /old_locus_tag="HBN41_19280"
     CDS             3138..3554
                     /locus_tag="HBN41_RS19280"
                     /old_locus_tag="HBN41_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_025464995.1"
                     /translation="MNNDWLSEKITYISALKNPSDAQKLLLELAKIQYRTPDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     ILLKTAEIDPNDMKWQIWKELGQETLNHRKKDKK"
                     /name="conjugal transfer protein TraD"
     gene            3715..4212
                     /locus_tag="HBN41_RS19285"
                     /old_locus_tag="HBN41_19285"
     CDS             3715..4212
                     /locus_tag="HBN41_RS19285"
                     /old_locus_tag="HBN41_19285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031380830.1"
                     /translation="MATAIRADYCTNMTINNCSFSGFNNCIEMNFCTNFNILGSGFFNS
                     NNAVFLSNCYGKGAISNNYTHNVNNLISVADSKLKYPLPSKNKEKHKIIKKEQKKNSSI
                     RNIEFNKKIKELINLEANQKFIKRNHQLACKIKYIKANLGKLEAESLLFLTQALMQYDL
                     QS"
                     /name="hypothetical protein"
     gene            complement(4704..5483)
                     /locus_tag="HBN41_RS19290"
                     /old_locus_tag="HBN41_19290"
     CDS             complement(4704..5483)
                     /locus_tag="HBN41_RS19290"
                     /old_locus_tag="HBN41_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_169057934.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEALNLLNSVAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     ARG             complement(4708..5483)
                     /name="aph(3')-VI"
                     /gene="aph(3')-VI"
                     /gene_name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.84"
                     /coverage="100.0"
     ARG             complement(4708..5483)
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.84"
                     /coverage="100.0"
     Typing          complement(1783..2702)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acccaaagcg tcgcacttcg tgctttggct ttggaaacgg ttgagagagt ttcggcaata
       61 ctctccctaa aggcggtcat ttaaaaaaat gacgcatcct ttaagtattg ccgatttacc
      121 gcatattctt ttggcttcgg agccacgtat atcggtcgta tccgcacata gctttgcaaa
      181 ctatgcagac aaggactaaa ggtcttgcac ctctccccac acgctccctg tcgaagtatg
      241 gcggtatctc tgcgagggtt tccccaacat acaacttaac caacctatac aactactctt
      301 aacgctccgc ttttgtgaga ttcccctgta gcggtttagc ctttcgacta tcgctacgtt
      361 gcaccagtga cacttattcc ctccacatgc aacgcatgcc aatccgtaaa acggacacgc
      421 aggagcaggc aaagctcgat ctaggatgct tatttgatga tttaaaacac ggattaacga
      481 agacttatca ttttgagtct tgatctaaac actgtttatt gtgaaaatag agttcaggta
      541 caagcctttg tgtgaaatct gcacgagttg gtagctctgc tgatcttcgt tacttgatgt
      601 tccaccatca aataactgcg taccgctgaa tataaagcga aagcccattg agttgcaaga
      661 ttcaatgggc tttttctttg atcaatcaga tattaagaca aaaaagagag gtcatatcta
      721 atcatgacta aatccctaat caagcacgtt tcaatgccat aatatggcga accatatcgt
      781 taatatgttg ttttgtttca ttatcttgtg aacttaacca tgcaactaca tcagcatcaa
      841 aagcctgtgc ttgagctatg gtatggcgtt cctgtaattt cttcacaaag ctaggtttaa
      901 ccaatggcgc attggtaggg tcaaaatttt tatagcgttc tagttcattt tgcattgctg
      961 tacctccgct tttggtgatg tgatggttca aatgcagtga ttattctcgc aacatctcca
     1021 cgttcaaccc aaaccactgt taatagtcta ccacgattgc ttaaaccgac tgatattagt
     1081 cgttgttcat catagtgtcc tacatcctca aaagagatca atgcaggatc aaggaaaata
     1141 ctacagacct cctcgaacgt gatttctcgc cctcgataga ctaaatccat tttttggtca
     1201 tgccattcaa ataacaagcc gtaatattca attaatggca tgattacctc ttttcctctc
     1261 gtgaaataaa aaatatgcct attattttgg gtctatgaca caaaaatctc cattttttcc
     1321 atatccgcac tgctttttca tcacacgaac acacggttta gattcctctc caacagtaca
     1381 gcttccaata tcaaactgga taccttgctg tcttaagctc tcgatctgag ggatattttc
     1441 taaaggtttg atataaacac tgaataacca aatgatgcca agcatcagaa tagcgaaaat
     1501 gcctaaaata atggtgatga ttttccatga aaattgatca ctagttgatt caatcgagtt
     1561 ttttaaatgt ccaagtgcct tagcggtctt gataagctct gctgtaccct cctgtaagcc
     1621 cgtatttagc gattttgata cagtgctatg ggtttgctct gcgatgacct tctgaatgtg
     1681 ttctttcgtg atttgaagct ctttaacggc ctgattgact aggttctgct gttgttctgc
     1741 aactgccatc agtgcatata actttttttc atcaggtgtc atcggctcat ccctctaaac
     1801 ttcattgttt tctgttgctc tcgttctaat ttctgctgtt ctttgtactg ctgtaaggca
     1861 agctgtttaa agctggcaaa atcagcctta aaactctcaa tgccctgttt aatttctttg
     1921 tctgagatag ggctgttttt gaggtctttc aggtcgtttt tagggtcgcc taaatctagt
     1981 cgctcaagct ctttataggc tgtacgtgac tgttgcaagg cttccctgat ttcagggtct
     2041 ttggcttggc ttggtaagag tttcttttca gggtctttct ctatgccttg ctctttgtag
     2101 cttcgcatat caatacggct gtcaatttgg tgtttttcca aatggctatt gcataaatcc
     2161 gcccaccttt ggcgtaggtc tttaatatca gtttttcgtt cctggtaact cttgcctgta
     2221 ttgtcttttt tagctccgcc acgttcagga ttcttgctgt tatagcgttt gaaatattgc
     2281 tctggatctc gttcgattcc atcttgcaga cgttcattaa acatgaggtg gatgtggggc
     2341 tgatcgttgc cgtccattgc tttagggtta tgaattgcaa actgatacgg atattttgag
     2401 ccaatttcag actgaataaa gtcttcaacc agttctaggc gttgctcagc attcatctct
     2461 ctaggcaggg caatttcata ttctcgatag gtactgccat ttttacgctc gtaaaggtcg
     2521 gctgcttgcc aaaaggtaat cggattatgt tcagcccatt tcggcatatt tccatagtca
     2581 ctatgttcaa ggtcggattg ttcctgtttc agcttttttt cttcgtcccg atcaatatat
     2641 tcggcatgcg gtgctgcctt acctgcctta ccaactttca cacttaaacg agctatcgcc
     2701 attaggtttt ttcctgtgcc ttttttcggt ctagttcttt cttaaatgtt ctttgatcga
     2761 gtatcgaagc ccacatcata atgatgataa ttagcggtgc tgcgatcacg ccaaaactaa
     2821 tatctttgat ttttcccaat acctgtttta gttgcatgct gttgctccta gctttggtac
     2881 ttcttttttt cattcaattt tcaattgaac gaaaaaaaga agtttcaggg ttttagggat
     2941 gaagtcccta acgtgcttag gtttctcaat gaaattgaga aacgtctaag cgcgccatca
     3001 aatttttgat ggaagtacag gcgataatct ccagaacgac tttaacaaaa ttacgttact
     3061 cgaacgtaaa tattttgtta tcgttctttg agtattgagc ctgtgctttt gcggtatatg
     3121 aattaatgga attgaatatg aataatgatt ggctgtctga aaaaatcaca tatatttctg
     3181 ctttaaagaa tcctagtgat gctcaaaaac ttttacttga attagccaaa attcagtaca
     3241 gaacacccga ccaagagaaa aaaatcaatg ccttgatcaa agctgaaaaa gccatagatc
     3301 gagcaaataa gcaaaaagtt gcagttagaa aattattgaa tgccgaaaag gaagcagaac
     3361 gtaaagctcg tacacgtcat ctcattcaat taggcgcact atttgaaata gctaacctag
     3421 atcagcgtga cccagccgaa ttacttggca ttcttctaaa aactgctgaa attgatccta
     3481 atgatatgaa atggcaaatt tggaaagagc taggacaaga aactttaaat catcgtaaaa
     3541 aagacaaaaa gtgattgttc agaattttta ttcaacttta tctaagcacg taaaaaactc
     3601 atctatatac agagggtcta aaaattatat ttttgattta taaagataat taaagattac
     3661 tttgcataag gtgtattatc ttaataataa ggcaactaag gtaggatgaa ttttatggcg
     3721 actgcaattc gtgcagatta ttgtacaaat atgactataa acaattgctc tttttcaggg
     3781 tttaacaatt gtatagagat gaacttttgt actaacttca atatattagg tagtggtttt
     3841 tttaattcta ataatgctgt ctttttatct aattgctacg gaaaaggagc tatatcaaac
     3901 aactatactc ataatgtaaa taatttaatt tcagttgctg attcaaaact aaaataccct
     3961 cttccttcaa aaaataagga aaagcataaa atcataaaaa aagagcagaa gaaaaatagt
     4021 tcaataagga atatagaatt taataaaaaa attaaagaac ttattaactt agaagctaac
     4081 caaaaattta ttaagagaaa tcatcagtta gcttgtaaaa taaaatatat aaaagcaaat
     4141 cttggaaaat tagaagctga aagccttctt ttcttgactc aagcattaat gcagtacgat
     4201 cttcaaagtt aagatgatgg tatgacaatt ttatatactc cataaaccct ttaaattaat
     4261 taggtggttt atgtcgcact tcaagtttta ctctgccaag tattgatatt attaaagctc
     4321 ttaagataat ttaacattcg tcatttccca taagaattta ataaaatact atatggctga
     4381 cagatagata aaagactaag aaaagaaaag cagacaaaaa aataccatcg ataataaaat
     4441 tggttaaaaa tgttgcacga tgaaatttaa ataaacttgc aaagctaata aattaacact
     4501 tttatattca aaaaaactta aatcacaagt taagctaata aacagtttta gctattttaa
     4561 ataagctcat tttaaaaacc aaaatttaga tttattggtg atataaaact ttaaatcaat
     4621 agctccacta acgctcttca cttatttatt taaaatttag cgacgtaaaa agacaattaa
     4681 gtatttttag ataatgcttg gaatcaattc aattcatcaa gttttaaaaa ataattcctt
     4741 ttgtcaggtc tatcattttt taaatgcttt aaaaatattt tagcagtttc ctccgatgca
     4801 tcctctctta ggcaacgttc aacaaaggat atatctacaa attcatctgc taacccagca
     4861 cggccaagat ctaaaaaata aatttcattg aatttatcta taaaaatatt actatccgtg
     4921 atatcgccat gagaaaaaac caatctttct tcaacacgag tctcatttaa ctcattccat
     4981 agacttaggt aagttttatg gtctccccat aattcagcgt caaaatcatc ttgatctata
     5041 tcgtcaagga gttggttatc aataaaaaat tttgactctt ttaaccgatg atcaatgttt
     5101 gaaataaatg gacaatcaat aatagcaact gaatttaaca gattgagtgc ctccttatag
     5161 atagcaagca attcttggtc tgttaaaaaa agcgctgaaa ttggttttgc attgatcgct
     5221 ttagtgatca tgaattcaaa ctgctcatcc tgaaaagtca tgatgagttc aggcaccttt
     5281 aatttctcag agagccaact caacattttc gcttcgcgag agacactgta tgtggtctct
     5341 gtatataaag tgctagatcg cttaagaaaa aaagtttcat tatttcgatt aaaagaataa
     5401 acatccgatg gcgactgacc aattttattt ggctctaaaa cactgtttcc aataaattgt
     5461 tgaataatat tgggcaattc catcaagttt tccttttatt cagcattaaa accccgcaaa
     5521 tgcaaggcct agtaaataga tgatcttaat ttggttcact gtagcaaaaa tatggggcga
     5581 attcaaaact ggttcaatag cctaagcaga aaaaggagta tttgattatc cgcactacta
     5641 tacttcctaa aaaaaaatgc tctcaacttc tcaactcgt
//
