LOCUS       NZ_CP050408             5432 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Acinetobacter baumannii strain VB2486 plasmid pVB2486_5, complete
            sequence.
ACCESSION   NZ_CP050408
VERSION     NZ_CP050408.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14414761
            Assembly: GCF_012935005.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., Francis,L. and
            Kirubananthan,A.
  TITLE     Complete genome sequencing for A.baumanii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., Francis,L. and
            Kirubananthan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 143.3824081x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 05:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,177
            CDSs (total)                      :: 4,081
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 35 of 107
            Pseudo Genes (incomplete)         :: 72 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050408.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VB2486"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pVB2486_5"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            complement(join(5252..5432,1..725))
                     /gene="trbL"
                     /locus_tag="HBN34_RS20745"
                     /old_locus_tag="HBN34_20745"
     CDS             complement(join(5252..5432,1..725))
                     /gene="trbL"
                     /locus_tag="HBN34_RS20745"
                     /old_locus_tag="HBN34_20745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="WP_204832353.1"
                     /translation="MSWLLMNSATFAEAIVNSFRQAASTASGYSLLTPSDFFVNGWKIV
                     QEILNSVSAWSPIDSLGMLIASIIIMIAFALICAFLVLALVEMYIVVSASVLMMGFAGS
                     QWTKDYALKTIQYAVSVGAKIFILQLIAGLGLTFIQEWATGFNVEDNVSLVGMIGCSVV
                     LLALTKIVPDMLQGVINGSSIGSGAALTSALRAVAAATAGAAAGAAGASMATKGAGALA
                     SEQLKTAQATGTGPSSAMGKAGFMAGNMAMNMSSALKSDLGSRLSGSTTGGRMAGSMMN
                     KANELRSKRSKSNQDENTIS"
                     /name="trbL"
     gene            complement(747..1535)
                     /locus_tag="HBN34_RS20750"
                     /old_locus_tag="HBN34_20750"
     CDS             complement(747..1535)
                     /locus_tag="HBN34_RS20750"
                     /old_locus_tag="HBN34_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="WP_005071978.1"
                     /translation="MPDLIQLEYLQEKLQQLLAESLFAIYLYGSAVDGGLGPESDLDVL
                     VVVTQPLTSALREQLAQELLKISQPVGELQRPLEVTILLKDEIQSGIYPLSYEMQFGEW
                     LREELKEGGILSSQKDPDISILLRKARSHHTVLFGPALNQWAPEISDQELWQAMSDTYP
                     EIVAHWDEDADERNQILALCRIYFSLVMKDIASKDNAARWVMSQLSDEQKFVLQRLIQE
                     YRGEIGKQNWQEEHYALQPIVNFLSSKIEEQFEQKRNLIT"
                     /name="ANT(3'')-II family aminoglycoside
                     nucleotidyltransferase"
     gene            complement(1539..1814)
                     /locus_tag="HBN34_RS20755"
                     /old_locus_tag="HBN34_20755"
     CDS             complement(1539..1814)
                     /locus_tag="HBN34_RS20755"
                     /old_locus_tag="HBN34_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004919011.1"
                     /translation="MKIESLEQQISKQQERLKQLKAQKQAAVARERSKQKELERKNDTR
                     RKILIGSCMLKIVENDTVAHEKLMAQLDKYLTENRDRELFNLPLKE"
                     /name="hypothetical protein"
     gene            2023..3441
                     /gene="mobQ"
                     /locus_tag="HBN34_RS20760"
                     /old_locus_tag="HBN34_20760"
     CDS             2023..3441
                     /gene="mobQ"
                     /locus_tag="HBN34_RS20760"
                     /old_locus_tag="HBN34_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_065996350.1"
                     /translation="MAIFHFSVKNISRSDGRSAVACAAYRSGEKLKDERYGKEQDYTKK
                     TGIEFKNIYAPENTKKELLDRETLWNTVETIETRKNSQLSREFEVAFPCELNQEQREKM
                     LNELCGKIVERHKVVVDAVIHAPHTAGGSDDRNYHAHIMLTTRSINQQGDLDKKTREFN
                     DNGKQEVEHWRQQFAELCNQFLEQVGSHERVDHRSYKEQGLDLEATQHEGSTITQLRRQ
                     GKDTEISLSNDAIKARNTERQLIKGLEQEILATERLVHDLEQTRENLDQEQRQKEKDQE
                     QRSPEELTKIAYSVISQYNDAIATEAKNIASSIQKTEIEQINAVLEQIELSKSDIKHQK
                     EELGKRPLFFGGKWDTANAEIQKQLNQLEEQERELRNNSPRFEPERYTDRAKLTVNKAN
                     PNLKAKYDRARNYLEREQQRKLAEQEKRKQEAKAIQEQEKQAFFARKALREQGKHEEAN
                     KMLEAEKRNRPRSR"
                     /name="mobQ"
     gene            4043..4981
                     /gene="repM"
                     /locus_tag="HBN34_RS20765"
                     /old_locus_tag="HBN34_20765"
     CDS             4043..4981
                     /gene="repM"
                     /locus_tag="HBN34_RS20765"
                     /old_locus_tag="HBN34_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_065996348.1"
                     /translation="MKKDLVVKDNALINASYNLDLAEQRLILLAILEARESNIPSDRDL
                     TIHAESYINHFNVHRNTAYKVLKDACKNLFERRFSYQKLTAKGNLENVMSRWVQRVSYV
                     ENEALVRIRFSDDVVPLITNLEKHFTSYELEQVSSLTSAYAIRLYELLIAWRSTGKVSM
                     LETKELRSRLGVLDTEHQRMESFKRRVLEPAIQQINDHTDIKAEYEQHKRGRSIIGFSF
                     SFKQKSKPKTINHERDPSTVDMFCNLSDSQINTYSSVLSKVHSISDLAGNKDYQAFAIW
                     IANILRDPSSVREETAKRIFKALRTETDFKG"
                     /name="repM"
     gene            4997..5230
                     /locus_tag="HBN34_RS20770"
                     /old_locus_tag="HBN34_20770"
     CDS             4997..5230
                     /locus_tag="HBN34_RS20770"
                     /old_locus_tag="HBN34_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065996352.1"
                     /translation="MTDLSKLSETELLAIWNKDNFGLKGQLYKLEQEYIEAKEILDNWQ
                     NKDHPLYERHQSCVDGLITRIERIKQQLNKCE"
                     /name="hypothetical protein"
     ARG             complement(751..1535)
                     /name="ant(3'')-IIa"
                     /gene="ant(3'')-IIa"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(3'')-IIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.47"
                     /coverage="100.0"
     Typing          2009..3438
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttacccattg cactcgatgg gcctgtgcca gttgcttgag ccgttttaag ctgttcactt
       61 gccaacgctc cagcgccttt tgtagccata gacgcaccag ctgcaccagc agcagcgcca
      121 gctgtcgcag cagccacagc acgtagggca gaagttaaag cagcaccact tccaatagat
      181 gaaccgttaa tcacaccttg gagcatatca ggcacaattt tggttaatgc cagcagtacc
      241 acactacagc caatcatacc gaccaaactt acattgtctt caacattaaa gcccgtagcc
      301 cactcttgga taaaagtaag tccaagacct gcaatcaatt gtaagataaa tatttttgca
      361 ccgactgaaa cagcatattg aatagttttc aaggcatagt ctttcgtcca ttgcgaacct
      421 gcaaacccca tcattaagac actagctgac accacgatat acatttcaac caacgccaaa
      481 accaagaacg cacaaatcaa ggcaaatgca atcatgataa tgattgaagc aatcagcatc
      541 cccaatgaat caataggcga ccaagcactt accgagttta aaatctcttg gacgatcttc
      601 cagccgttca caaaaaaatc tgagggtgta agcagactgt agccactggc ggtagatgca
      661 gcctgcctga agctatttac aatcgcttca gcgaacgttg ctgagttcat caaaagccat
      721 gacataaagc cagaatcaaa aaattattat gtgatcagat ttcttttctg ctcaaactgc
      781 tcttcaattt ttgaactcaa aaaattgaca ataggctgca aagcataatg ctcctcttgc
      841 caattttgtt tgccgatttc ccctctatat tcctgtataa gtcgctgcaa tacgaatttc
      901 tgctcatcag aaagctgaga cataacccat cgtgcagcat tgtcttttga agcaatatcc
      961 ttcatcacta aactaaaata gatccggcat aaagctaaaa tctggtttct ttcatctgca
     1021 tcctcatccc aatgcgctac aatttcggga taagtgtcag acattgcttg ccatagttct
     1081 tgatcagaaa tttcaggtgc ccattggttt aaagctggcc caaataaaac tgtatgatga
     1141 gatctcgctt ttctaagcaa tatactaata tctgggtctt tctgtgagct caatattcca
     1201 ccttctttaa gctcttcacg cagccattca ccaaattgca tttcataact taaaggataa
     1261 attccagact gaatctcatc ttttaataaa atagtaactt ctaatggcct ttgtaattcc
     1321 ccaacaggct gtgaaatttt tagtaattct tgtgcaagct gctcacgtaa agcagaagtt
     1381 aatggttgag tgaccacgac cagtacatca agatcacttt ctggccctaa accaccgtca
     1441 acagctgaac cataaagata aattgcaaat aatgattccg ctaaaagctg ctgtaatttt
     1501 tcttgtagat attctaactg aattaaatca ggcatcactc actcctttaa tggaagatta
     1561 aataattctc tatctcgatt ttcggttaag tatttatcga gttgagccat taacttttca
     1621 tgagcaacag tgtcattttc gacgattttt aacatacaag agccgattaa aatcttacgt
     1681 cttgtatcat tctttcgttc taactctttt tgcttagaac gttcacgagc tacagcagct
     1741 tgtttttgag cttttaactg ttttaaccgc tcttgttgct tagaaatttg ttgttccaat
     1801 gactcaattt tcatctttaa catacctttc aaatagagga aatcccccta aatcataagc
     1861 gcacttgaac attgctttca agttgctata tgattgttgt gggttttccc gaagggcgca
     1921 ctttcccaaa ctaaagtttg gagtgcgtaa ggggacaccc cttaaccccg aaaaaaaatt
     1981 ttcaattttt tttaaaaata cttgtcaatg ggactttaca tcgtggctat ttttcacttt
     2041 tcagtaaaaa atattagtcg atcagatggt cgaagtgctg ttgcgtgtgc tgcatataga
     2101 tcaggggaaa aattgaaaga tgaacgctat ggaaaagagc aggattacac gaagaaaaca
     2161 ggcatagaat ttaaaaatat ctatgcgcct gaaaacacca aaaaagaatt attagatcgg
     2221 gaaacgcttt ggaatacggt cgaaaccatt gaaactagaa aaaactctca attatcaaga
     2281 gaatttgaag ttgcatttcc ttgtgaatta aaccaagaac aacgagagaa gatgctcaat
     2341 gagctatgcg gaaaaattgt agaacgtcat aaggtcgttg ttgatgcagt cattcatgcc
     2401 ccacataccg caggcggaag tgatgaccgc aactatcacg cccacatcat gctcactaca
     2461 cgcagcatta accaacaagg cgaccttgat aaaaaaacga gagaatttaa cgacaacggc
     2521 aaacaagaag tcgaacactg gagacaacag tttgcggaac tctgcaatca atttcttgaa
     2581 caggttggca gccatgagcg tgtcgatcac cgcagctaca aagagcaagg tttagattta
     2641 gaagccacac agcatgaggg ttctacaatt acccaacttc gcagacaggg caaggacact
     2701 gaaatcagtt taagtaacga tgcgattaag gctagaaata cagaacgcca attaattaaa
     2761 ggacttgagc aagaaatttt agccacagaa cgccttgttc atgacttgga acagaccagg
     2821 gaaaatttag accaggaaca acgccaaaaa gaaaaagacc aggaacaacg ttcccctgaa
     2881 gaattaacca aaattgcata tagcgttatt tcgcaatata acgacgctat agccacagaa
     2941 gcgaaaaaca tagcgtcatc tatccaaaaa acagaaatcg agcaaataaa cgctgttttg
     3001 gagcaaatag agctatctaa aagcgatatt aaacaccaaa aagaagaact aggaaaaaga
     3061 cccctgtttt ttggcggaaa atgggataca gcaaatgctg aaattcagaa gcagttgaac
     3121 cagctggaag aacaagaacg agaattgagg aataactccc ctcgatttga gcctgaacgc
     3181 tatacagatc gggcaaaact gactgtaaac aaggctaatc caaacctaaa agctaaatat
     3241 gaccgagcaa gaaattatct tgagcgagaa caacaacgga agttagccga acaggaaaaa
     3301 cgaaaacaag aagccaaagc catacaagaa caagaaaaac aggcattttt tgcacgtaaa
     3361 gccttgcgtg aacaaggtaa gcatgaagaa gccaacaaga tgcttgaagc cgaaaagcgg
     3421 aatagaccac gttcaagata ggtatatcta tggtgcaaaa tatatgattt tgcaccatag
     3481 atatacctat tctttttggt gtttattaaa attatctaaa caatacatgg ttttacagag
     3541 cgagtgtcta cgagcgaact agcaatattc gaccctcgac cctctgaaaa accgctttta
     3601 aatgacttga accctagaaa atctatattt tcatgttgaa atctgcacct agaccgagcg
     3661 aagcgagcat aaagccttat gagcgaagcg aattcatagt tgcttttgct tttagatttc
     3721 acaaacagga ttaaccgaaa aattgcccct cgaagcgagc ttgcgagctt caataaagta
     3781 cgagctttag cgagtacata gggcaatttt gacccctccc ctctttttta atttttttat
     3841 tttttttaat ttttagggtg actgaaatcc ttatatagca atggtttcag actttatata
     3901 gcaacgttta tcttgcaata tagcaacgtt tatcttgcaa tatagcaacg tttatcttgc
     3961 aatatagcaa cgtttatctt gcatattaca acattgttat ataatgttgc tataacaaca
     4021 aaattcgaat aagttgctat gtatgaaaaa agacctagtc gttaaggata atgcgctgat
     4081 caatgccagt tataatttag accttgcaga acaacgccta atccttttag caatactgga
     4141 ggcgagggag tctaatatcc ctagtgaccg tgatttaact atccatgcag aaagctacat
     4201 taaccatttc aatgtccata gaaacaccgc ctacaaagtc ttaaaggatg cttgcaaaaa
     4261 cttgtttgag cgtcgcttta gttaccaaaa attaacggct aaaggcaatc ttgagaatgt
     4321 tatgagccga tgggtacagc gtgtgtccta tgttgaaaat gaagcccttg tccgtatcag
     4381 gttttcagat gatgttgtgc cactgatcac caacctagag aagcatttca ccagttatga
     4441 actggagcag gtaagtagcc taacgagtgc ctatgccata cgtttatatg aactactaat
     4501 tgcatggcgt agcactggga aagtatcaat gcttgaaact aaggaattga ggtcaagact
     4561 tggtgtattg gacacagagc atcaacggat ggaatcgttt aaaagacgag tattagagcc
     4621 tgctattcaa caaattaacg accataccga cattaaggca gaatacgagc aacataagcg
     4681 aggacgttca atcattgggt tttcattcag ctttaaacag aaatccaagc ctaaaacaat
     4741 caaccatgaa cgagatccaa gcacggttga tatgttctgc aacttgtcag acagtcagat
     4801 aaatacttat agctcagtat tgtctaaagt ccacagtatc agcgacctag cagggaataa
     4861 ggactatcaa gcatttgcaa tatggattgc caatatttta cgtgacccgt ccagtgtgcg
     4921 agaagaaacg gcaaaacgca tattcaaggc attgcgaaca gaaacagatt ttaaaggtta
     4981 aatgggagta ttactaatga ctgatttatc aaaactaagt gaaaccgagc tattagctat
     5041 ctggaataaa gataattttg gactgaaagg tcaactatac aaactagaac aagagtacat
     5101 cgaagccaaa gaaatattgg ataactggca gaataaagac cacccacttt atgaacgtca
     5161 tcaaagttgt gtggatggtc ttattacaag gattgagcgt ataaaacaac aactaaacaa
     5221 atgcgaataa tcagaaaaaa tctgtctcgc cctatgagat agtgttttca tcctgattgc
     5281 ttttagatcg ttttgaccgt agctcgtttg ctttattcat catcgagcca gccatacgtc
     5341 caccagtcgt agaaccactt aaacgactac caaggtcact ttttaaagca gaactcatat
     5401 tcatggccat attaccagcc ataaaaccag ct
//
