LOCUS       NZ_CP050406            12574 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Acinetobacter baumannii strain VB2486 plasmid pVB2486_3, complete
            sequence.
ACCESSION   NZ_CP050406
VERSION     NZ_CP050406.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14414761
            Assembly: GCF_012935005.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., Francis,L. and
            Kirubananthan,A.
  TITLE     Complete genome sequencing for A.baumanii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12574)
  AUTHORS   Veeraraghavan,B., Vijayakumar,S., Amala,A., Francis,L. and
            Kirubananthan,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7; Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 143.3824081x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 05:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,177
            CDSs (total)                      :: 4,081
            Genes (coding)                    :: 3,974
            CDSs (with protein)               :: 3,974
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 35 of 107
            Pseudo Genes (incomplete)         :: 72 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 17 of 107
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050406.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VB2486"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pVB2486_3"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Christian Medical College"
     gene            complement(join(12405..12574,1..535))
                     /locus_tag="HBN34_RS20640"
                     /old_locus_tag="HBN34_20640"
     CDS             complement(join(12405..12574,1..535))
                     /locus_tag="HBN34_RS20640"
                     /old_locus_tag="HBN34_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042893601.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1008 family transposase"
                     /protein_id="WP_001067790.1"
                     /translation="MNPFHGRHFQGEIILWAVRWYCKYGISYRELQEMLAERGVNVDHT
                     TIYRWVQRYAPEMEKRLRWYWRNPTDLHSWHMDETYIKVKGRWTYLYRAVDQRGHTIDF
                     YLSARRNSKSAYCFLGKIFNTVKKWQIPRVINTDKAATYGHALSRLKREGKCPVDIEHR
                     QIKYKNNVIECDHGKLKRIIRATLGFKSMKTAYATIKGIEVMRALRKGQASSFYYGQPQ
                     GEVCLINRVFGL"
                     /name="IS6-like element IS1008 family transposase"
     gene            complement(603..935)
                     /locus_tag="HBN34_RS20645"
                     /old_locus_tag="HBN34_20645"
     CDS             complement(603..935)
                     /locus_tag="HBN34_RS20645"
                     /old_locus_tag="HBN34_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282109.1"
                     /translation="MKFQFIFENFKALPNGTDAFKQLKILCEQEIGNNNNLDNSALFLI
                     YGFAKNYVLLYEDQEVTPEFAKKAKSQLIDYMSELNIALISQDSNLILETLNNISKKYF
                     KSSRIF"
                     /name="hypothetical protein"
     gene            1179..1790
                     /locus_tag="HBN34_RS20650"
                     /old_locus_tag="HBN34_20650"
     CDS             1179..1790
                     /locus_tag="HBN34_RS20650"
                     /old_locus_tag="HBN34_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_015060246.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            complement(1980..2864)
                     /locus_tag="HBN34_RS20655"
                     /old_locus_tag="HBN34_20655"
     CDS             complement(1980..2864)
                     /locus_tag="HBN34_RS20655"
                     /old_locus_tag="HBN34_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(2920..4395)
                     /gene="msr(E)"
                     /locus_tag="HBN34_RS20660"
                     /old_locus_tag="HBN34_20660"
     ARG             complement(2924..4395)
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4888..5160)
                     /gene="nadS"
                     /locus_tag="HBN34_RS20665"
                     /old_locus_tag="HBN34_20665"
     CDS             complement(4888..5160)
                     /gene="nadS"
                     /locus_tag="HBN34_RS20665"
                     /old_locus_tag="HBN34_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369781.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTG
                     LSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
                     /name="nadS"
     gene            complement(5153..5473)
                     /locus_tag="HBN34_RS20670"
                     /old_locus_tag="HBN34_20670"
     CDS             complement(5153..5473)
                     /locus_tag="HBN34_RS20670"
                     /old_locus_tag="HBN34_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897307.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILHQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5682..6119)
                     /locus_tag="HBN34_RS20675"
                     /old_locus_tag="HBN34_20675"
     CDS             complement(5682..6119)
                     /locus_tag="HBN34_RS20675"
                     /old_locus_tag="HBN34_20675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031985418.1"
                     /translation="MAKLRNRGKASNDRSEEQREKNDSNLQTKITLGFDFFLGQDTKGT
                     GQTWEDWHTSGLIVSMLHKLKHLCTLTPAQAKNEGSLKIYGAFPPCSKFKCPSHLKEID
                     SWGTIRQMGNGGKTRIAGFYDKNYVFRIVFLDKNHEFWPTD"
                     /name="hypothetical protein"
     gene            complement(6112..7491)
                     /locus_tag="HBN34_RS20680"
                     /old_locus_tag="HBN34_20680"
     CDS             complement(6112..7491)
                     /locus_tag="HBN34_RS20680"
                     /old_locus_tag="HBN34_20680"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_031985417.1"
                     /translation="MLVNFSCENILSFKNEVSFSMLASQKKKDNILTNNFFMAGKEQQE
                     PILETSLIFGANGSGKTNFIATLAYLKRIALNQNPENKFIAPFRLNNDSKKTPSELEIE
                     FYAKNNIKYRYGISIFKGEIIEEWLYYTPQTRETILFHREKNSLIEYNSAGFIEAKDFI
                     DENQKISDKLKNDTAFIFLLSTFNGEHSNAISEWFSNIVILDIEDKKDNLKDTLYYWKD
                     NNDFQNWARPILNSLGICDLKFDHKVESVEDIVKQIKSDQEKLKNSNEKNKELKDKAIN
                     LFDNLLDFVTSSSLEKTEDIDFNSVKVIKNIDGNEFPIPLYLESSGTIKLIHLLSALYR
                     SSKNGEILLIDEFDVKFHTLLSKYIFKKYHDINNKGQVIVTVHDTNLMDTNYFRRDQIW
                     LINKNPKGESELYSLIEFKELASNINNKNYSTEYLTGFFHAIPLFNEDDVDSLMEDNSN
                     G"
                     /name="ATP-binding protein"
     gene            complement(7786..8403)
                     /locus_tag="HBN34_RS20685"
                     /old_locus_tag="HBN34_20685"
     CDS             complement(7786..8403)
                     /locus_tag="HBN34_RS20685"
                     /old_locus_tag="HBN34_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_120752772.1"
                     /translation="MKSLSVIELSNLYGITRQAIYKQVNKGNLSKNSDGKIDLSEAIRV
                     FGEPSRNVNSSQTTETRKLSEVHLLEQQVYMLQKQLEQAHEREQFQREELKAKNDQLHV
                     KDEQIEAIQRLLEAPKAYTTSSIDPKLDIATDTRSKSESNYDVLTTQPRETPVEVEIQT
                     QPKDDGLTTPEQVENKRIPVPKHIEPEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(8480..9379)
                     /gene="repM"
                     /locus_tag="HBN34_RS20690"
                     /old_locus_tag="HBN34_20690"
     CDS             complement(8480..9379)
                     /gene="repM"
                     /locus_tag="HBN34_RS20690"
                     /old_locus_tag="HBN34_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650500.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004763517.1"
                     /translation="MMSTSKKELVVKSNQVIEASYQLSSTEQRIVLAAISKISRAEDIT
                     DDEIYRVTIDDLKKLGVHEKTAYRDLKDGVNRLYDRSINLAIDDESIKMRWIQSIRFLE
                     SKSVVGIRFSKEILPFISNLSREFTKYSLSDIAGMSSAYAIRIYELLSQYRSIGKREIS
                     IESLRSMLELGKRYPLSADLKRWVIDTAVVQINEHSPLNVSYEQIKTGRKVTHIHFTFK
                     EKSKSIEHKSEQNNFYKLTDSQINMFGNQLSRLHEVSHLAQQGESYDDLAIKIKDMLRD
                     PIKQKQLIPHLKNLGFKA"
                     /name="repM"
     gene            complement(9911..11089)
                     /locus_tag="HBN34_RS20695"
                     /old_locus_tag="HBN34_20695"
     CDS             complement(9911..11089)
                     /locus_tag="HBN34_RS20695"
                     /old_locus_tag="HBN34_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_120752771.1"
                     /translation="MASYHFSVKSKNKGYALGHYLYISRLMQYEGIRKTSNETVEHIEP
                     GRCMPSFVKDPIEFWQAADTYERANAKAYIEYEIALPNEFTPEQRKTLIETFFDKHIVP
                     QQYPHSYALHNVKSRLSGEEQPHCHLMFSLKADDGIERTAEQYFKRYNPQNPAKGGAKK
                     IQLQDGHADYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEINGTLYLPEPKLGAGKQNETMEYLAEIQKIRLHNAKEREMEQKQRQQTQQHEHFYSC
                     LNEPYTSLEVMYYLNYLVKKAKNQKELNQDFNEIIKTQKPILQAEHWNLNKMDYQLKNS
                     LQYTDPQIEFNPVTVLKSQVEQPQVVQGNQPTQPSIQPKPKNNDYDFGGPGF"
                     /name="MobA/MobL family protein"
     gene            11394..12176
                     /locus_tag="HBN34_RS20700"
                     /old_locus_tag="HBN34_20700"
     CDS             11394..12176
                     /locus_tag="HBN34_RS20700"
                     /old_locus_tag="HBN34_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_120752770.1"
                     /translation="MEMEKAKKRGRPAQLLQRAELHAFVDFLLENNRRTELQNQVIDAL
                     QAEDFNFEMLSEAQKILVKEALKPYREHLKLQLLFDELSKNPRQTEYEEKFLELYKRYQ
                     KDTLDPAETNILKTMCTRYLNFKNQKLEYSDLELYLSQIQKKDEGKKRKAENQRKFEVG
                     GGVLSAYKIKNDDALDTENFLKRVQADRYLAGKLRQTKIYQKVWASDLNNKEKLELLFE
                     VLNQLALYGNKGENVKFVDFAIEAAQTKLKQKTNSVAE"
                     /name="hypothetical protein"
     ARG             complement(1984..2864)
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2921..4395)
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1981..2864)
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(8481..9376)
                     /name="rep_cluster_1357"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.649"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9915..11011)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.902"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acttaccatg atcacattca atgacattat ttttatactt aatctgcctg tgctcaatat
       61 ctactggaca ttttccttct cgctttaacc gtgataaagc atggccatag gtcgctgctt
      121 tatctgtatt gatgacccgt ggaatttgcc attttttcac cgtattgaag atttttccta
      181 aaaaacaata ggctgattta ctgttccgtc tagcggaaag gtaaaagtca atcgtatgac
      241 cacgttgatc aactgcacga tacaggtaag tccatcgccc cttcactttg atataagtct
      301 catccatatg ccatgaatgt aaatctgtag gattacgcca ataccagcgt aagcgttttt
      361 ccatttctgg agcataacgc tgaacccaac gataaattgt ggtgtggtca acattcacac
      421 cccgttcagc gagcatttct tgaagttcac gatagctgat gccatattta caataccaac
      481 gaacagccca aagaatgatt tcaccttgaa agtgccgacc atggaaggga ttcatctgct
      541 gtatctcgaa ataaataaga tatttagctt atcatgtcag cctatttgca acagtgccag
      601 atttaaaata ttcttgaact tttaaaatat ttttttgaaa tattattaag agtttctaat
      661 ataagattag aatcttgact gattagagca atattaagtt cagacatata atcaattaat
      721 tggctttttg ctttttttgc aaattcagga gtaacttctt gatcttcgta caataagacg
      781 tagttcttcg caaacccata aataagaaat aaggccgaat tatctaaatt attattgttg
      841 ccaatttctt gctcacataa aatttttaac tgcttgaaag catctgttcc atttggtaaa
      901 gctttaaagt tttcaaatat aaattggaat ttcattttgg ggactcactt aaaaagaaac
      961 tctacagctt tagtcactct caatcaatat ctaatttttc caaaattaat atgatacgta
     1021 ttatacgaac tcccaaagca tggctttcag taatgctttt tctatcaaaa gagtatacct
     1081 gaatgatagc gagccaaatc gcatacaata tctatcatta gacttaatgc aataattaaa
     1141 tgatagtatt atcattaatc taatagaatt aagattaaat gatagaaatg aatacacgta
     1201 tttatctacg agcttcaact aaagatcaag atgcagaaag ggctttgcag attcttcagg
     1261 atctaaacca aaacttgaat ttgggtgaaa ccattgtgta cgtggaaaac tatagtggta
     1321 cgaagttaga ccgacctgaa ttgaataagc tactgtcaga agcaaatcaa ggtgacacat
     1381 tgttagttga aagtattgac cgcttatcac gcctaaccca acgagatttt caagagctga
     1441 agcgcaggat ccaggagaag ggacttcgtt tagtcgtagc agatcttcct actacctacc
     1501 aaatgattca aaccagtgac agcattactc attcaatctt ggaactcatc aacaatatgt
     1561 tgattgatct tctggcaaca atggcccgcc tagacaatga gaaacgtata gaacgtatta
     1621 agcagggcct ggcacgttcg ggttacaaac caacaggcaa gaaggcaaat gaggctaaac
     1681 ataaacgaat aaaagaattg ctagtagttg gcaatatgac taaggaagaa attgccaaag
     1741 cagtgaattg tggagttgca actgtctatc gagttgctaa agttatctaa aagaggctta
     1801 tctcaacgcg cccccaatgg ggcaccgctg cattcaggtt acttcacata agaaatttta
     1861 tgttaaatga ttgttagaaa tgccccttga tcgaggcatt ttctataaag aatactaaaa
     1921 actaattaaa gccaaccaag tttcaaaaca aggcagttct cagcaatcat atttttaaat
     1981 tatataactc ccaactgagc ttttgctcca acgataaggc tttcattttg agtttctaag
     2041 gcaaataaac catacatcaa aggagaggcc gctgctcttt ctaaagtctg ttcatatagt
     2101 ttattccaaa ctttaccccc tagtttttca tactcgatga ttagagtttt gaggctttct
     2161 tctccaaaca aagttacatg cccagcaaaa tcaatcgctg ggtcatctat atgggctgtt
     2221 gaccaatcaa taacgcctga aacagctcca tcctttgaag ctagtacatg cccagcatat
     2281 aaatcgccat gtataaattg ggtgaaatct gcccatagaa catcattatc caaccatttt
     2341 ctgtagcggg tttccaattg ctcacttata ccaatttcag attttactaa ctgcaaattg
     2401 tttgctattt caggtcttaa atctgaaggt ttcataattt tcaaatcatt ttcccgaact
     2461 tctttttcag gaatactatg gatttcaaat aaggtttttg ccaaagatgt tatgtatttc
     2521 gggctatctt tgtccatatt ccaaattatt tcataggttt cagcatccaa atttaaaaca
     2581 ggattatctt taagtatggg ataagccact aattctgtag atgaaattct ccaatcagga
     2641 acctctacag aaagatgttt ttttaccaat tctaaaatgc gtttttcttt cttgatttgt
     2701 tccctcatgc catcacgacg aggaatacgc agcaaccatt gttgcccctt tgtatcaaga
     2761 gcaaaaacga ccttaaaatc aatgcccatt tcattgaaat tcattttgtc cgtaagcaac
     2821 aagccgtgtg cttcagcaag tgattgaata tcttgaattg tcatttttaa tttcctttaa
     2881 agagttcaat aattaatgtt cggattagat tggctatcat taacaatctc tctcaaaagt
     2941 cttgatgatt ttgtggtctt tgatctcgta gataatgtca gcaatattat cgaccaattg
     3001 cttgtcatga gatacgaaga taatagttcc tgcataggac ttcatcattg tttctaatgc
     3061 ggcaatactt tttaggtcaa gatagtttcc tggttcatcc ataagcaaaa tattatattt
     3121 tcctaaaagc attttggata aaagcagttt gatgatttca cctcccgata agtcggataa
     3181 gtttttttga atatcattcg ctccgatccc cattgaagcc aatactgcac gaatttccgc
     3241 aactgtgtac tcgcactctt cctgcataaa ggagagcaca gatttatgcg tgttaaattt
     3301 atatcctgtt tgtgtaaagt agccaatttc agcttttgga gatatggtta atccatcagc
     3361 acgttctgat atcattttta acaaggacgt tttccctgtt ccattcgatc cagttatagc
     3421 gactttagcg ccaagcggta ttataaagtt agcgtcatca aagatagtac ggctaccaaa
     3481 ttttaagctc agaccatctg ccgtaatcgg gaacttattg tgcagttcta gggctgaact
     3541 ttgacgaaaa cgaatagaac gcaaatgctc tggtgcttga atatcttcta atgcagccaa
     3601 acgcttttcc atactcttag ctgcctgata cagttttctt tgcttggtgc cagtcatttt
     3661 tgcatgccca agtcgtccag cactttcggt agagtttttg gatttttctc cttttttctt
     3721 attgtctaat cgattagctt gctggcgttt ttcttgcaca gcagattcta atcgctcccg
     3781 ttccttcatc atcagctcat attctacggc ttggtgttgt cgctcttctt ctttttgacg
     3841 caagtaatcc gagtaaccac cccaatattc cgtaatttta ccgtctttta actcccatat
     3901 cttgtctaca accatatcaa gaaaatatcg gtcatgactg ataacaagta atgctccatc
     3961 aaatgcttta agttgaccaa taagtagatc tattccattg agatcaaggt ggctggttgg
     4021 ttcatccgct agaatgccat gtacttgttg ggaaaatgcg gcagcaattt ttgcacgagt
     4081 ttcctctccg ccactcattg tgtcgttttg tacattggaa acaccaaggc gagataacat
     4141 tgcccggtct tcgaccgttt ctatttcgat tccgcccagt tggctgatat gtgcaaaatc
     4201 accaaaacgc tgtaatgtcg cttcggctaa aacaatttcg ccattaagta ctttgagtaa
     4261 actactcttt cctgctccgt tatcacccac aagaccaata cggtcataag agtgaatttc
     4321 caattcatca atatccaaaa catcacgccc agcataatcc aagcgtatgt ttctcgcttt
     4381 aataattaaa ctcattttta tttactcctg tttagctctt gaaatttttt atgcagcaaa
     4441 caggatttag gtgaaaacaa aagctagcat cacagtgtcc tcccaaaaaa aaagctattc
     4501 atccacaggg tggacaaata gctagtcaat taagttataa ctggaaacta tgcactaaaa
     4561 gcatacttat aaattaagca tacttttact ttatatatcc gcatatattc ttaaagacac
     4621 aacaaaagcc caccattata aaatagtgtc actatgcaaa tagttgtgtc ttaacgaatg
     4681 cggatagaat gcataaactt acctaaaaaa taaaattcag ttttatgata actttactgt
     4741 taaaagaagt ctagacaacc ttgttttatg cttggatata aggcttattt taaaataata
     4801 gtagtgaaga ttttctaaaa ttaacataat acaccttata cgaaataaaa atgggagcct
     4861 taagctccca ttttttaagt aattttttca aataaggctt gggtgagcat ctaaaattcg
     4921 aatcaatgta gctgctggtc cagttggata gcgtcgtccc tgttcccaat tttgcagggt
     4981 acgggggcta atatgtagac gtgcagcaaa ttcattttgg ctcaagccag ttttttcacg
     5041 tacttcttta atatcaggca attcgagttc tgtaactcgg cttgctttaa cttctttacg
     5101 tttgatagct ccagcttctt tgattgaagc aaccaagtca tcaaataagt tgttatccat
     5161 gaaattgctc cttcacgagt tggtgcagaa tggcggtttc cttatctgtt aaattatctt
     5221 ttactgattt tggataagcc actaagaaaa agatctgatc atcctcggtg acccaataat
     5281 aaatcacccg tatcccacca cttttccctt tgttaccttg agcacaacgt actttgcgaa
     5341 taccaccgcc attcttgatt aggtcacctc tatcaggctg taccaaaaga tcttgctgaa
     5401 gttgacgata ttcctcatca gatacaaggt ctttaatttg cttggtaaag atgctggttt
     5461 caataaataa catggactct ttatacgtca atggcgtatt tgaattttag cagtttccat
     5521 aacaaaaaac gagagctggt agatctcgtt tcgcataacc gccattatgt taaatggact
     5581 gtaaattggt ggaaaattcc agtttttaaa attgcgaata agagatttta tgttaagtac
     5641 aagaacactt aataaagtgt tcttgtacat ttttagatat tttaatctgt aggccaaaac
     5701 tcatgatttt tatctaaaaa aactattcgg aatacataat ttttatcata aaaaccagca
     5761 atacgggttt tccctccgtt tcccatttgt ctaatagttc cccacgagtc gatctctttt
     5821 aggtgtgaag gacatttaaa tttagagcag ggagggaaag cgccatatat ttttagagac
     5881 ccttcatttt tagcttgggc tggtgtcaga gtacaaaggt gtttgagctt atgtagcata
     5941 gagactataa gtccacttgt atgccaatct tcccatgttt gccctgttcc cttagtatcc
     6001 tgaccaagaa agaaatcaaa acctaaagta attttcgttt gtaaattact atcatttttt
     6061 tctctttgtt cttcagatct atcgttactt gctttgcccc tattacgcag tttagccatt
     6121 tgaattatct tccattaaac tatctacatc atcttcatta aatagtggta tagcatggaa
     6181 aaaacctgtt aaatattctg ttgaatagtt cttattatta atatttgatg ctaattcttt
     6241 gaattctatt aatgagtata gctcactttc gcctttaggg tttttattta taagccaaat
     6301 ttggtctctt ctgaaatagt ttgtatccat taagtttgta tcatgaacag ttacaattac
     6361 ttgtcccttg ttattaatat catgatattt tttaaatata tatttagaga gtaacgtgtg
     6421 gaatttaaca tcaaattcat caattaataa tatttctcca tttttagagg atctatatag
     6481 tgcacttaat aaatgtatta atttaatagt accgctagac tctaaataaa gagggatagg
     6541 gaattcatta ccatctatat tttttataac tttaacacta ttaaaatcaa tatcttctgt
     6601 tttttccaaa gatgatgatg ttacaaaatc tagaagatta tcaaaaagat taatagcttt
     6661 atcttttagc tctttatttt tttcgttact atttttaagc ttttcttggt cgctcttaat
     6721 ctgttttaca atatcttcga ctgattcaac cttatggtca aatttcaaat cacatatacc
     6781 taatgagttt aaaataggtc tagcccaatt ttgaaaatca ttattatcct tccagtagta
     6841 caaagtatct ttaagattat cttttttatc ttctatatct aatataacta tattagaaaa
     6901 ccattctgaa atagcattag aatgttctcc attgaatgtt gatagaagaa agataaaagc
     6961 agtatcattt tttaatttat cacttatttt ttgattttca tcaataaagt cttttgcttc
     7021 aataaaccca gcactattat actctatgag actatttttc tctcgatgga ataatattgt
     7081 ttctcttgtt tgaggagtgt aataaagcca ctcctcaata atttcgcctt taaaaataga
     7141 aataccataa cgatatttga tattattttt tgcataaaac tcaatttcaa gctcagaagg
     7201 agtcttttta ctatcattat ttaatcgaaa aggagctata aatttatttt ctggattttg
     7261 attaagagcg atccttttta ggtacgccaa tgtagcaata aagtttgtct ttccgcttcc
     7321 attagctcca aaaataagtg aagtttctag aatgggttct tgttgttctt tcccagccat
     7381 aaaaaaatta ttcgttaaaa tattgtcttt ctttttttga gaggcaagca ttgaaaatga
     7441 tacttcattt ttaaaagaaa gaatattttc acaagaaaag tttactaaca taaatttttt
     7501 caattaaggc tacgtaaaat tagatcttaa atcatttttt ttaaaaaaaa agctattttt
     7561 taaaaataag gtttatgtag atattgggtt tttgtagttt ctctaaaatt aacataatac
     7621 accttatacg aaatgcgtta taattatctt tagaaatcaa agctgtagtt cctagttgat
     7681 agcccatctt ccccaaggtg ggcttttttt atgatttttc acgttccacg cctattcttt
     7741 gatcaatgtt tcacgacttt attcatatta gttaaacaat cgtggttaat catagggtct
     7801 aaaaaaacgg ctccaaaagc ctcttttttc aggctctggt tcaatgtgtt taggcactgg
     7861 aatccgctta ttttccacct gctccggagt agtcaatccg tcatctttcg gctgagtttg
     7921 gatctctact tctacaggag tttctcttgg ttgagtagtc aatacatcat agttcgactc
     7981 actttttgac ctagtatctg ttgctatatc taattttggg tcgatagaac tagttgtata
     8041 agcttttgga gcttctagta ggcgttgtat tgcttcaatt tgctcatctt taacgtgtaa
     8101 ctgatcattc ttagctttta gttcctcacg ttggaactgc tcacgctcat gagcctgttc
     8161 aagctgtttt tgaagcatgt aaacttgctg ttctaatagg tgaacttctg acaactttcg
     8221 tgtttcggtt gtctgtgaac tgtttacatt tctagaaggt tcaccaaaga ctcgaatagc
     8281 ttcggaaaga tcaattttac cgtcactatt tttacttaaa ttacctttat taacttgctt
     8341 ataaatggct tgacgagtta ttccatagag gtttgaaagc tcaataacag atagtgattt
     8401 catcttgtaa ttttggttgt ccattatgtg aactaaaata cattaacaaa cagtcaactg
     8461 ctacatacac aatcgacctt taggccttaa agcctaaatt tttaagatga ggaataagtt
     8521 gtttttgttt tattggatct cgcagcatat ctttaatttt aatagctaag tcgtcataac
     8581 tttcaccttg ttgtgctagg tgagatactt catgtaagcg tgaaagctga ttaccgaaca
     8641 tattaatttg tgaatctgtc aatttataaa aattattctg ttcggattta tgctcaatac
     8701 tttttgattt ttctttaaac gtgaagtgaa tgtgtgtgac ttttcgacct gtctttattt
     8761 gctcgtaaga tacatttaaa gggctatgtt cattgatttg aactacagca gtatcaataa
     8821 cccatctctt taaatcagca gacaatggat atcttttccc taactccaac atacttctta
     8881 gagattcgat ggaaatctct cgttttccta tggaacgata ttggcttagt agctcataaa
     8941 tacgaatagc atatgcgcta ctcataccag caatgtcaga tagcgaatac ttagtgaatt
     9001 cacggcttag attagaaatg aacggcaaaa tttctttaga aaaccgaatt cctacaacgc
     9061 ttttactttc taaaaagcgt atggattgaa tccatctcat tttgattgac tcatcatcaa
     9121 tagctaaatt aattgatctg tcatataagc ggtttacacc atcttttaga tctctataag
     9181 ctgttttttc atgaacccca agttttttta aatcatcaat agttacgcga taaatttcat
     9241 catctgtaat atcttcagcc cgactaatct tactaatagc tgctaagaca atccgttgtt
     9301 ctgttgagct gagttgatat gaagcctcaa ttacttgatt tgatttaact actaattctt
     9361 ttttagatgt actcatcatt tttaattcga taaactaact atgagtaata taattctctc
     9421 atagttggta gtcaatccct catagttaag tagtcaatcc ctcatagtta gtagtcaatc
     9481 cctcatagtt agtagtcaat ccctcatagt taggagctga aagcctttct gttaaagcct
     9541 ttcagatagc ctaaaagatt taaaagattt aaaatatatt aaaaaggata agaatgaaaa
     9601 attgcccttg gttttcgcta cgctcaaact ctattgaatc tcgctttcgc tcgattcgtc
     9661 ggggcaattt tttggttaat accctctaga atttaataaa aagcaaaagc agtaggaatt
     9721 cgctacgctc ataatttttt tatcttcgct tcgctcagtc atgacttaaa aatatcaagt
     9781 cgctttgctc tgtacttgag tcttagcgac aaaagggagc ttgtaatcag ggaaaaaaac
     9841 ataacgagaa ttttgattgc ctcagttcgc tcgtagacac tcgctctgta aatgcctaca
     9901 agattcatat tcaaaaacca ggaccaccaa aatcgtaatc attatttttc ggcttaggct
     9961 gtatggatgg ttgagtaggt tgattgcctt gaacaacttg aggttgttca acctgtgatt
    10021 ttaagacagt aacaggatta aactcgattt gaggatctgt atattggagt gaatttttta
    10081 gttggtaatc catcttattc aaattccaat gttcggcctg aagtattggc ttctgtgttt
    10141 tgatgatttc attgaagtct tggttgagtt ctttttgatt tttggctttt ttgaccaagt
    10201 aattcaagta atacatgacc tcgagtgagg tatacggctc attcaggcaa gaatagaaat
    10261 gttcatgctg ttgcgtttgt tgccgctgct tttgttccat ttctcgttct ttggcattgt
    10321 gcagccggat tttctggatt tcagccaggt attccatggt ttcattttgt tttccagcac
    10381 ctaatttggg ttccgggagg tacaatgtgc cgttgatttc attatatttt tcataactgt
    10441 cagcagaaac ttggtttttg attgtgatgt cttggccatc caatgtgtag gtgacggtag
    10501 ggcagtgctg tgcaagggca tcattcagat ggttttccca ttgttttcgg atatagatta
    10561 aaaaagtgga atagtctgcg tggccatcct gcagctggat tttcttggca ccgcctttgg
    10621 ccggattttg aggattgtag cgtttgaaat attgttctgc tgtgcgttca atgccgtcat
    10681 cagctttgag ggagaacatg aggtggcaat ggggctgttc ttcgccactg agacgcgatt
    10741 taacgttgtg tagggcgtat gaatgcggat attgctgtgg aactatatgt ttgtcaaaaa
    10801 acgtctctat gagcgtttta cgctgttctg gcgtgaattc gttggggagg gcaatttcat
    10861 actcgatgta ggctttggcg ttggcacgtt catacgtatc tgctgcttgc caaaattcga
    10921 taggatcttt aacgaaagaa ggcatacaac gaccaggttc aatgtgttct acggtttcgt
    10981 tgcttgtttt acggatgcct tcatattgca taagtctgga aatgtagagg taatggccaa
    11041 gtgcatagcc tttatttttg cttttgacgc tgaaatggta gctggccatg ttcacctttt
    11101 tgttggtctg ggaccggttt ttatggagtg aaacgagatg aaaaccgccc ggatttggaa
    11161 tgcctggatg gctgaaaaat ccggttgaag ggggtcaagg gggaagcgcc ccattgcgta
    11221 ctagtttctc cgattaggag aaactgtcaa gtgcgaatta tttaaaataa aaataaaaat
    11281 tcttttcaag aaattttatt tttattttaa ataattattt tcagactgcc cgatttaatt
    11341 atggtaaaac cataattaaa tcggtagcag ttctgaaaat ttagcggcag atgatggaaa
    11401 tggaaaaagc aaaaaagcgt ggtcgaccag ctcagttact tcagagagca gagttacatg
    11461 ctttcgtgga ttttttatta gagaacaatc gacgtactga attacagaat caggtcattg
    11521 atgcgctaca agctgaagat tttaattttg aaatgttgtc tgaagctcaa aagattctgg
    11581 tgaaagaagc attaaaacct tatcgtgagc atttgaagct tcagttgctg tttgatgagc
    11641 tgtctaaaaa tcctagacag actgaatatg aagaaaagtt tttggaatta tataaacgat
    11701 atcaaaagga cactttagat ccagctgaaa cgaatatctt gaaaacaatg tgtacgcgtt
    11761 atctgaattt taaaaatcaa aagcttgaat acagtgactt agagttgtat ttgagtcaga
    11821 tccaaaaaaa ggatgaaggt aaaaaacgta aagcagagaa tcagcgtaag tttgaagtcg
    11881 gtggtggtgt tttaagtgct tataaaatca aaaatgatga tgcgttagat accgaaaact
    11941 ttttaaagcg ggttcaggct gatcgctatc tagctggaaa actccgtcaa acaaaaattt
    12001 atcagaaggt atgggcatcg gatcttaata ataaggaaaa attagaatta ctttttgagg
    12061 tgctcaatca acttgcacta tatggtaata aaggtgagaa cgtaaaattt gtggactttg
    12121 ctattgaggc agctcagaca aagctaaagc aaaaaactaa ttcagttgcc gagtaattta
    12181 tatgtttatc ctaagtagaa gtgtcctata tggaagcttc tacttagggc taacagatga
    12241 atgtcgtata tgagttttta tgtaaacttg gagtacaccc taaagtaacg tcatggcggc
    12301 cccaatcctg aacctaaaat caatatctta gaagccgtgg aacatcaatt ttggcactgt
    12361 tgcaaataga gatttgagtc gatgaagttc ctttttaaaa gtgcttagag accgaaaacc
    12421 ctgttgatta gacacacttc accttgaggc tgaccataat aaaatgacga tgcttgtcct
    12481 ttacgtagtg cacgcatgac ttcaatacct ttaattgtgg cataagccgt cttcatagat
    12541 ttgaatccta atgtggccct gatgatccgc ttta
//
