LOCUS       NZ_CP050369             4715 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 47733 plasmid p47733S, complete
            sequence.
ACCESSION   NZ_CP050369
VERSION     NZ_CP050369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14421539
            Assembly: GCF_011769725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Chudejova,K., Kraftova,L., Mattioni Marchetti,V., Hrabak,J.,
            Papagiannitsis,C.C. and Bitar,I.
  TITLE     Genetic Plurality of OXA/NDM-Encoding Features Characterized From
            Enterobacterales Recovered From Czech Hospitals
  JOURNAL   Front Microbiol 12, 641415 (2021)
   PUBMED   33633720
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbila Assembly v. SMRTL Link v.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011769725.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,842
            CDSs (total)                      :: 5,717
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 66 of 175
            Pseudo Genes (incomplete)         :: 113 of 175
            Pseudo Genes (internal stop)      :: 20 of 175
            Pseudo Genes (multiple problems)  :: 22 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050369.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="47733"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p47733S"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.08 N 14.36 E"
                     /collection_date="2018-12"
                     /collected_by="Charles University in Prague"
     gene            complement(join(4024..4715,1..175))
                     /locus_tag="HB636_RS29580"
                     /old_locus_tag="HB636_29595"
     CDS             complement(join(4024..4715,1..175))
                     /locus_tag="HB636_RS29580"
                     /old_locus_tag="HB636_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(159..1115)
                     /locus_tag="HB636_RS29585"
                     /old_locus_tag="HB636_29600"
     CDS             complement(159..1115)
                     /locus_tag="HB636_RS29585"
                     /old_locus_tag="HB636_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            2191..2385
                     /locus_tag="HB636_RS29590"
                     /old_locus_tag="HB636_29605"
     CDS             2191..2385
                     /locus_tag="HB636_RS29590"
                     /old_locus_tag="HB636_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3039..3152
                     /locus_tag="HB636_RS30190"
     CDS             3039..3152
                     /locus_tag="HB636_RS30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427893.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(3374..4042)
                     /locus_tag="HB636_RS29595"
                     /old_locus_tag="HB636_29610"
     CDS             complement(3374..4042)
                     /locus_tag="HB636_RS29595"
                     /old_locus_tag="HB636_29610"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_223195384.1"
                     /translation="MGYIQMIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCS
                     EWNMKILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYN
                     KSGVLLTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFD
                     LNDENKEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS
                     "
                     /name="PP2C family serine/threonine-protein phosphatase"
     Typing          complement(1677..1932)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(2057..2155)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(2459..2543)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          2554..3111
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.831"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtgtatct atcactacca tgcttaacac caagaagagt ccctcccttc ataaccatca
       61 tttcattcaa actctcctct atagaaatag atttaagtat tatatgtaca gcctggcgca
      121 tcaacctgta cttcccacca gactcttcga tccaattatt aatttctttt tccataagac
      181 ttaaccatat caacattaat agaaataaac cattcttcat caaagtaagg tgagaatgga
      241 ctctttggag atagcacttt cgaactacct ctgtatttac ccgcctcttg tttccattcg
      301 cttatttttt tattattaac cccaataatt ctatccaaaa tgaatccaac ccttaccttg
      361 tcaatatgac ggccaagctt atcgacttcc tttataatca gaggtgcata ttttttaccg
      421 tgctcaataa atgattccaa tacatgctcc tcaccaccac agtaatcagg ccgcctggtc
      481 atatccataa aggtcttacc gatagatgat accctgacag gggaattacg agccttcatt
      541 ggctcaacat aatttgtttc ttgtgtaacg ataattttta ttccattgac ctcagaattt
      601 cttggaaatc taggcatgaa ttgtttggct tcgtctatat caggattatc cttttgaaaa
      661 tcatgataga acttattctt ccactgttcg gaacgacatg tcgtaaagtg gattatcttg
      721 ggtattttat ctgtgatgtt atgccaatcc atagcggata aataggataa gtatccatat
      781 ggatacgtag agcacactat atcattttcc ggagaaccat cgttaccttt aacaaaaaag
      841 acctcataga aaccgccaat taaatggggg taagcatgat gcttatcgtt actttcaact
      901 cttgataaaa cccctgactt taataatttt aatatatttt tttcaaattc attaggtcct
      961 gctttgttct ttctaatttg atatatcctc tcaccattat attctttttt taaataaaaa
     1021 aggtaaatct gcaaagagat ttcttctttg gttatgactg gttttttata ttcattcaac
     1081 aaccaagacg ttagcgcttc ttcgataaga tacatattca accttttcgg tttagtctct
     1141 aatagaatat acgtcctaag gacgtatatt ctattagaga ttgattgtca agaaaaaaat
     1201 aataacaaac aagcatttgc catttttata tttttgatat cctataaaat aaatcctaag
     1261 gatttatttt ataggataat aagtaggaag tcaagaaaaa gtttacttat atcaccgttt
     1321 tcataacacc tcatgcgatc aattcagcgc cggttatagt tcaaatccat gattaggaca
     1381 tctgttgctc ataaacgatg agcgtaaaag atcatttttc agcgtcattt accataacgt
     1441 cgcattacgc gcaccgctaa aacgcgctga acgcgattct gccgcgaaca ccgcaaaaag
     1501 agatgttctc cccggggtaa accggaaaaa tgcgtcagga tcgctttcag ctcactgcat
     1561 agctatgcat gaaagtgaat gacgatcggt ttgggcgcat acagtgccca tgagacacat
     1621 tttcggacat tttcagttcg tgaatccctg actcattgct acatatctga acagacaccc
     1681 ctagcagcgc ccccaggggc gtcctgtaaa aaaacacacc gcgccgctag cagcacccct
     1741 aatataaaat gatgtttttt atgaaaatag tcagtaccac ccctaaaaag cggtgttggc
     1801 gcagctacaa caccgcgccg acaccgcttt tttaaatatc acaaagagag caagaagaac
     1861 taatttgtca taactctcta tttataaaga aaaatcagca aaaaattgtt tttgcgtggg
     1921 gtgtgggcat ttcaggcatg agaacagact cgcgtttttc cggagtatca gcggaattat
     1981 tgattaagaa acactcgacg cagagcgaaa ttttatgcaa cagcggctgc actgcacagg
     2041 gattatttat cagttgatat gctgtgccat gatacgtcct aaaaaaacag gacaggatgt
     2101 gcaattgtaa taccgtcgcc cgcaacgtct tacaattacc acctggtcag ggtccacccg
     2161 acacccggta ctgacggaga acaacggaag gtgaacaaac agcaacaaac tgcgctgaat
     2221 atggcgcgat ttatcaaaag ccagagcctg acactgcttg aaaaactgga tgctctggat
     2281 gccgacgaac aggcggccat gtgcgaacga ctgcacgaac tcgcggaaga actccagaac
     2341 agcatccagg ttcgctttga gaccgaaagt gaaacaggaa cataacgaag ctgacggagg
     2401 cgctcacggc ttgtccgcga gcgttttcgg cagcagacaa gcccgccagg gcgcgtcagc
     2461 gggtgtcggg gcgaagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     2521 actatgcggc atcagtgcga attgtatgaa aagtgcccca tgccgggtgt gaaattccgc
     2581 acagatgcgt aaggagaaaa tgcgcgtcca ggcgcttttc cgcttcctcg ctcactgact
     2641 cgctacgctc ggtcgttcgg ctgcggcgag cggtactgac ttacacaaaa acggcagcac
     2701 ggttatccac agaatcaggg gataaggccg gaaagaacat gtgagcaaaa gaccaagaac
     2761 aggaagaagg ccgcgccgca ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 agaaatcgac gctcaagtca gaggtggcga aacccgacag gacttaaaga taccagacgt
     2881 ttccccctgg tggctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc
     3001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3061 ccgactgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgcct
     3121 taacgccact ggcagcagcc actggtaact gatttagagg agttagtctt gaagtcatgc
     3181 gccagttaag gctaaactga aagaacaggt tttggtgact gcgctcctcc aagccagtta
     3241 ccttggttct tcgagccgca aactctttga acctaagaga aaaccgcccc gcaaaggcgg
     3301 tttttttctt ttacagaaaa gagattatct atatgacacg aagatctcaa tagcttcttc
     3361 aaaaaacacg aaatcaagaa gggaatctaa cgtagcaaac cgtatgattc tctgcgttac
     3421 ccgggatatt tttagtaaat ttagccaagt tagattctgg gttatctttc atgaggtcaa
     3481 taacagtacc atcattaaaa tgctcccaaa acccatcagt acataaaata acctctttgt
     3541 tttcatcatt taaatcaaaa actgaatgat tgaaaattat ctcatcagag cagaaatcaa
     3601 caaatgatgt tagataattt ctgtctgggc aataacaaac gaggtgaggt atttttttat
     3661 tatcgattcc attttccttt aatcttttcc aggcaaccga gttatcctct gttagaagga
     3721 ctccactttt gttatataca cggcaatctc ctgcgttata tatatggagg tgcgtattat
     3781 ttttaaaaag gaaacatact gcagccttaa caggaactga cttgcatggt tcaataatat
     3841 ctttattcag atcaccagaa aacacgcact tatctaacct accagagaga attttcatat
     3901 tccactcaga gcaggaatac ccatccataa taaccattcc aaacacatca ttttgacaat
     3961 ttatcgtaga taataagtct aagttttcac atctgttacc aatatcacta aaactgaacg
     4021 ctatcatttg aatatatccc acggaagact ttcatagaaa caggcaactt tttcataagc
     4081 atcttcaaaa agaggtaatg atttaacggt ggatgataaa tcctgatatc gctgactgga
     4141 acgctcgata atttctacat cccttatacc tttaacgttt aagagattat ccaattgttt
     4201 accttcactc ttttttaaaa gagactccag tattttatac ttatcttgcc gagaaaactc
     4261 atatttacct aaaagataat tgatatcata tatatcttgc tctcgatttc tctctctaac
     4321 tttttgttgc aaaacggatc gatatttttc agccatcaaa tcagttaagc tgtaggtctt
     4381 tacgctatca ttgtcatcat caccatctaa tgataaaaag tcaaaatcat acgttttttc
     4441 gttaaagcta aaatcgatat ttatcactga aggtgattga ttagattcaa gccttttcat
     4501 tgcagacgca ttagaacgat cgcaaaaggc tatatttatc tgtacagttg gaaatgttcc
     4561 ttgtgcatta ggttgaattt tatgagattg aactttacat ttaataccat atcccaattc
     4621 agcctcagca acatccattg agtcattaaa cctattgaaa aaatccccca agtcaatttc
     4681 agaaagtttt ttttcggtag aaaaatcaat atcct
//
