LOCUS       NZ_CP050363             1546 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 47733 plasmid p47733_COLMG828, complete
            sequence.
ACCESSION   NZ_CP050363
VERSION     NZ_CP050363.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14421539
            Assembly: GCF_011769725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Chudejova,K., Kraftova,L., Mattioni Marchetti,V., Hrabak,J.,
            Papagiannitsis,C.C. and Bitar,I.
  TITLE     Genetic Plurality of OXA/NDM-Encoding Features Characterized From
            Enterobacterales Recovered From Czech Hospitals
  JOURNAL   Front Microbiol 12, 641415 (2021)
   PUBMED   33633720
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbila Assembly v. SMRTL Link v.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011769725.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,842
            CDSs (total)                      :: 5,717
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 66 of 175
            Pseudo Genes (incomplete)         :: 113 of 175
            Pseudo Genes (internal stop)      :: 20 of 175
            Pseudo Genes (multiple problems)  :: 22 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050363.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="47733"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p47733_COLMG828"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.08 N 14.36 E"
                     /collection_date="2018-12"
                     /collected_by="Charles University in Prague"
     gene            complement(join(1075..1546,1..5))
                     /locus_tag="HB636_RS27475"
                     /old_locus_tag="HB636_27490"
     CDS             complement(join(1075..1546,1..5))
                     /locus_tag="HB636_RS27475"
                     /old_locus_tag="HB636_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          85..193
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(390..471)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          586..843
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="3.93e-111"
ORIGIN
        1 aacatgattt tttccctctg aaaatcccat gcactaacgt tggttcaaat ctaagccatc
       61 atgacacatc tgtgcaacac cttgttgcgt gttgtcgcat ccaggatgcg acaacgttgt
      121 gtcaccctgg atgcgacagg taaacaaaaa acacactaaa aaaacaacaa cttacaaatc
      181 ggcgtaaaag atctattcta ggcaagggtg ttgcgcgcaa cgcaacgccc ttttggggtg
      241 tcggggcgaa gccctgaccc agtcacgtag cgatagcgga gtgtatactg gcttactcct
      301 ctgcaagcct cgtcagccat gattttgccc gatttcgcgc tctccacgtt caacagctcg
      361 atcatgtttt tgccgtccgt tcaccgttaa aacgcctcag aaatcgggac aggatgtggg
      421 aatgtcggac cgcgcacgcg cactcagaca ttccccacct ggtcagggct tcaccccgac
      481 accctgcatc cgtgcgtaag tgctcaatgt aacttccggt tctcttccgc ttcctcgtcg
      541 attcgctcgc tacgctcggt catcgacttg cggcgagcgg gatcacctct cgcatgttgt
      601 cggtgatccc gttgttgttc tttcggggat gagtcgtcag atagcccttc aaacacgctc
      661 agaagctctc agacgcacga aacgggttta aatgcccgcc agggcatgga aggcgcgaaa
      721 aacgcttaca gaggctctca ggaggtcagg cgcgccgata cccctaacca ggggctttca
      781 ggtcgctagg aaccgcgtca gctccctgca aagcctattt gcacggtaca ggcagctatt
      841 cggctggttg gtggtcgtag accgtcggtg ttacagcgat gggggtctgg ggttctcccc
      901 agacggtttc cggttgcgct caaggcgctg ccggaaccgt ggcgacgcgg tcgttcttgg
      961 gatcggggag caaaaacacg caagggattt tgaggcggca aaagagcaat gcaggataga
     1021 caatcatggt caaaacgtta acacgtagcc cctgattgtc cgcctgccgc tggctcaatt
     1081 tgaaaacatg gccatgattc ggcgctgaat atcaccgtct ttgttgacca gtgttttagt
     1141 gacaaacgct gcatggtttt tcgtagtgtt gtatcccttc cagacttcat gtggatcgag
     1201 ggcatacgca caaaccttac cagcaacggg gattctaact aatgcatttt ttgattctaa
     1261 gtaattgagc caccgaataa ttgtgcttct gctaactcca agtttttcag ctaggaattg
     1321 ctgatccgca acgacagcgc cacacgtagg atcgatatgc tccgctaaaa atgagtacag
     1381 cttcagtgct gatatgcctt ggctatcctt cagcagctct cgaacgcgtt cccaaccttt
     1441 tggggatacc tgtgtaaacc ttgaattctt ctttgcttct tcgagatcct cggtgttttt
     1501 ctttgcctca tctgaaagtt gtttcagtcg tttttctgaa tcaata
//
