LOCUS       NZ_CP050362             2101 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Klebsiella pneumoniae strain 47733 plasmid p47733_COLBS512, complete
            sequence.
ACCESSION   NZ_CP050362
VERSION     NZ_CP050362.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14421539
            Assembly: GCF_011769725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Chudejova,K., Kraftova,L., Mattioni Marchetti,V., Hrabak,J.,
            Papagiannitsis,C.C. and Bitar,I.
  TITLE     Genetic Plurality of OXA/NDM-Encoding Features Characterized From
            Enterobacterales Recovered From Czech Hospitals
  JOURNAL   Front Microbiol 12, 641415 (2021)
   PUBMED   33633720
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2020) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Microbila Assembly v. SMRTL Link v.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011769725.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 02:52:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,842
            CDSs (total)                      :: 5,717
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 66 of 175
            Pseudo Genes (incomplete)         :: 113 of 175
            Pseudo Genes (internal stop)      :: 20 of 175
            Pseudo Genes (multiple problems)  :: 22 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050362.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="47733"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p47733_COLBS512"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.08 N 14.36 E"
                     /collection_date="2018-12"
                     /collected_by="Charles University in Prague"
     gene            741..1706
                     /locus_tag="HB636_RS27465"
                     /old_locus_tag="HB636_27480"
     CDS             741..1706
                     /locus_tag="HB636_RS27465"
                     /old_locus_tag="HB636_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1710..1976
                     /locus_tag="HB636_RS27470"
                     /old_locus_tag="HB636_27485"
     CDS             1710..1976
                     /locus_tag="HB636_RS27470"
                     /old_locus_tag="HB636_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1135..1366
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gacaccaatg caggatagac aatccgatgt caaatgttaa cactctgcga gtggtacatt
       61 ttccccggat tatcgtcctg agcctgccgc tggctctctt tctaccgcct cgctttgctc
      121 gttgctcaac gcctcacaga cacggattaa aatccgcatc cgttcaccgt tttttaaagt
      181 ccgttaaaag catgatgcca tctccgagag ttaatctcgt caaatgctaa atcgtggggg
      241 tcccctttgg ggttccgatt tagtgattga cgacaccacc gattaaaaaa cttatgcggg
      301 gtggatggtt tcacgaagtg aggccatcca cctgtaagac agggttttgt ttttattccc
      361 tgttttggtg atcgggtgtg tggaaaaggt tggggtaagc cgttcggggg tgcttgtttt
      421 ggggggttaa aattgtggtt attttttgcg caattctcgc gcgtgatcct tgtatttata
      481 cttaagggat aaatggcgga tatgaaatag tggtttagcc cagtaatgac gaggctttga
      541 gtgggttttg acaggtcaaa gaaaatggag cagaattgag gcgtttttaa tcggcgttgg
      601 ggagtgcgtc aacactcccc aacatttcga atgtgtcacc tcagcggcaa actctggtga
      661 catgtactgg ctcgcaatgc acaggtacgt gatgaatata ccacatcaaa tcacagcctg
      721 cccagatcgg agcaggctta atgtcagaag ataaattcct ttcggactac agcccccgtg
      781 atgcagtttg ggatacccag cgcacgctta ccgattctgt cgggggtatc taccagactg
      841 ctgctgaatt cgagcgctat gcactccgta tggcctcctg tagcggtttg ttacgttttg
      901 gttggtctac catcatggaa accggagaaa cgcgcctacg gcttcgtagt gcgcaatttt
      961 gccgtgtccg tcattgccct gtctgccagt ggagaagaac cctcatgtgg caagcccgtt
     1021 tttatcaggc tctaccgaaa atcgttgtgg attacccgtc ttcccgatgg ttgtttctga
     1081 cgttaactgt caggaactgc gagataggtg aacttggaac agtccttaca gcaatgaatg
     1141 cggcgtttaa gcgaatggaa aagcgaaagg agctatcacc tgttcagggg tggatcaggg
     1201 ctacggaggt gacgcgaggt aaggatggca gcgcacatcc gcattttcac tgtctgctga
     1261 tggtgcaacc ttcttggttt aaagggaaga actacgttaa gcacgaacgt tgggtagaac
     1321 tctggcgcga ttgcttgcgg gtgaactatg agccgaatat cgatattcgg gcagtaaaaa
     1381 ctaagacagg tgaggttgtg gccaacgttg ccgagcaact gcaaagcgcg gttgctgaaa
     1441 cgctgaaata ctccgttaaa ccggaagata tggcaaacga tcctgagtgg tttcttgagc
     1501 tgacgcggca gcttcacaag cgccgtttta tctcgaccgg tggggcgcta aaaaacgtcc
     1561 tccagttgga tcgagaaacc aatgaggatc ttgtcattgc cgacgatgta ggggatggca
     1621 ctgatgacgg gaagcggacg gcgtttgtct gggattcagg taaacggcgt tacaaacgcg
     1681 cccctgagaa ggataaatcg gattaacgta tgaatattaa tattgaatac ctgaatggaa
     1741 ataagactat tggtttattt tttttaagaa gtgaagcggt gattcctgac aggtttaaaa
     1801 accttatttt gcttattgat ggattaagtt ttggcacatt tggttttcat ccgcacgaag
     1861 gttttgagga tgaattaatt ttatatattc agaaaacaaa cgagagggta aaaactcttt
     1921 ttgtgaaaat agatttaaac ggtatcaagt ttggattttt aagaacgcat tcttagttct
     1981 ggaaaagagc cagcggcagg ctgaggtgat aggtacgaga ttgcatgcaa tctctagtgc
     2041 tctgtctatc ctgcattatc ctcagcatta tcctcagcat tatcctcagc cttgccaact
     2101 c
//
