LOCUS       NZ_CP050359             5596 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ZRKP01 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP050359
VERSION     NZ_CP050359.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14411773
            Assembly: GCF_015239295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     In Vivo Selection of Meropenem Resistance among Ceftazidime-
            Avibactam-Resistant, Meropenem- Susceptible Klebsiella pneumoniae
            Isolate with KPC-33 Carbapenemase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Bioinformatics center, GensKey Gene
            Technology Co. Ltd., Life Science Park Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-DEC-2019
            Assembly Method                   :: Unicycler v. version 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 13:57:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,905
            CDSs (total)                      :: 5,782
            Genes (coding)                    :: 5,628
            CDSs (with protein)               :: 5,628
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 49 of 154
            Pseudo Genes (incomplete)         :: 108 of 154
            Pseudo Genes (internal stop)      :: 19 of 154
            Pseudo Genes (multiple problems)  :: 18 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050359.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZRKP01"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China"
                     /lat_lon="39.98 N 116.43 E"
                     /collection_date="2019-10-14"
                     /collected_by="Laboratory of Clinical Microbiology and
                     Infectious Diseases, Department of Pulmonary and Critical
                     Care Medicine, China-Japan Friendship Hospital, Beijing, P.
                     R. China"
     gene            complement(join(5500..5596,1..230))
                     /locus_tag="HB786_RS29265"
                     /old_locus_tag="HB786_30060"
     CDS             complement(join(5500..5596,1..230))
                     /locus_tag="HB786_RS29265"
                     /old_locus_tag="HB786_30060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(588..746)
                     /locus_tag="HB786_RS29825"
     CDS             complement(588..746)
                     /locus_tag="HB786_RS29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1386..1562
                     /locus_tag="HB786_RS29270"
                     /old_locus_tag="HB786_30065"
     CDS             1386..1562
                     /locus_tag="HB786_RS29270"
                     /old_locus_tag="HB786_30065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1552..1896
                     /locus_tag="HB786_RS29275"
                     /old_locus_tag="HB786_30070"
     CDS             1552..1896
                     /locus_tag="HB786_RS29275"
                     /old_locus_tag="HB786_30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2105..>2539
                     /locus_tag="HB786_RS29725"
                     /pseudo=""
     CDS             2105..>2539
                     /locus_tag="HB786_RS29725"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2574..3083
                     /locus_tag="HB786_RS29285"
                     /old_locus_tag="HB786_30080"
     CDS             2574..3083
                     /locus_tag="HB786_RS29285"
                     /old_locus_tag="HB786_30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3090..3302
                     /locus_tag="HB786_RS29290"
                     /old_locus_tag="HB786_30085"
     CDS             3090..3302
                     /locus_tag="HB786_RS29290"
                     /old_locus_tag="HB786_30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3969..4193
                     /locus_tag="HB786_RS29295"
                     /old_locus_tag="HB786_30090"
     CDS             3969..4193
                     /locus_tag="HB786_RS29295"
                     /old_locus_tag="HB786_30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4177..4443
                     /locus_tag="HB786_RS29300"
                     /old_locus_tag="HB786_30095"
     CDS             4177..4443
                     /locus_tag="HB786_RS29300"
                     /old_locus_tag="HB786_30095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4513..4863)
                     /locus_tag="HB786_RS29305"
                     /old_locus_tag="HB786_30100"
     CDS             complement(4513..4863)
                     /locus_tag="HB786_RS29305"
                     /old_locus_tag="HB786_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4929..5474)
                     /locus_tag="HB786_RS29310"
                     /old_locus_tag="HB786_30105"
     CDS             complement(4929..5474)
                     /locus_tag="HB786_RS29310"
                     /old_locus_tag="HB786_30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          1221..1300
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(638..1035)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1887..3418
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcttttaatg gtgaagggag cggtaactca atatttgaaa agtgattggc gagataggga
       61 tcaaggtttc tcatccattc aggcttttcg ccaatgcgtt cttcaactga aagtgaacct
      121 gataatctct ggttaataag atcacttaga ctcattttgg ttttattcga aagccgttgt
      181 aaggattgct ttaatcctat atctagcatg atatttgttc tttctctcat taccccccct
      241 tgctattgcg ttttttgttg cgcacaaagc taccattgcg cattaagaaa cgcaataaaa
      301 aaggagggag ggagtggttt gtacatgatg tgcagtgcgc tgagggcatc agtggcgggt
      361 gtttttccgt gggatggatg gagctccggg gataacagaa ctgacgatca tttaaaggat
      421 cttcttgaga tccttttttt ctgcgcgtaa tctcttgcac tgtaaacgaa aaaaccgcct
      481 ggggaggcgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg ggtaactggg
      541 tatacgagac cgcagatacc aaaactgttc tttcagtgta gccgcagtta ggcacccact
      601 tcaagacctc tcaatatctc tctcgtatat atccagttac caatggctgc tgccagtggc
      661 gtttttgcgt gtccgtccgg gttggactca agatgatagt taccggaaag ggcgcagcag
      721 tcgggctgaa cggggggttc gtgcatacag cccagcttgg agcgaacgac ctaaaccgag
      781 ccgagatacc agcagcgtga gctatgagaa agcgccacgc ttcccgaaag ggaaaaaggc
      841 ggaacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcacgagg gagccgccgg
      901 agagaaatca ccggcatctt taagtcctgt cgggtttcgt cctcctctga cttgagcgtc
      961 catttctgtg atgctcgtca ggggaggcgg agcctatgga aaaacggcaa cgccgcatcc
     1021 ttttctccgg ccctttctga tttgagcgcc tttgctttat cccgttatcc gtgagtcggt
     1081 cacgcccgcc gcagccgaac ggcaggagcg cagctaccga gtgagcgagg aggcgtcatt
     1141 tcatctgtcc gtgcatggta ttgcggacgc tcctgagcca catatcacac ctgccacatg
     1201 aggcactttc atgaatcgtg ctctcatgcc aacatagtaa gccagtatac acagcgtaaa
     1261 cgctgtgact ggttcagggc tgcgccccga aacccgctaa caccactgac gcgctggcgc
     1321 ttgtccaacg ccggagcgac ccggaaaact tttccgtgcc gtccggcgtt tgcgaaggag
     1381 ggataatgca gaagccgaag aagaagccgc tcgtcgtaaa aaaaagatcc tggcatccgg
     1441 cagtgatggc aaatctgatg cgtcaggcac aggaggaaga ttaccgccgg gagcagatgg
     1501 cagagcttga gcgttcagcc cggtcacatt ttgcggatat cggaggagag gatggcggtt
     1561 aagcgtaccc gttttttagg tatccgggtg accgatgggg agtaccagca gctgctggag
     1621 cgctgcaatg gaaggcaact ggcggtgtgg atgcgggaaa cctgtctcga tacgcgcccg
     1681 gcgcggtcgt tgcggctgcc gtccatcgat ccggttttgc tgcgccagct tgccgggatg
     1741 gggaacaacc tcaaccagat cgcccggaaa attaacggcg gccagtggtc aggcgctgac
     1801 cgggttcagg tggtggccgc gctgatggcc attgatgccg aactcgagcg gctgcggcat
     1861 gccgtgctgg aaaagggggc agacgatgat cgttaagttt catccgcgag ggcgaggcgg
     1921 cggtgccggg ccggtggatt atctgctggg gaaagaccgc cagcgcgacg gggccatcgt
     1981 cctgcaggga aagccggagg aggtccggga gcttatcgac gcctcgccct acgccaaaaa
     2041 gtacacctcc ggcgtcctgt cttttgctga acaggattta ccgcccggcc agcgtgaaaa
     2101 gctgatggcg agcttcgagc gggttctgat gcccggactc gataaagacc agtacagcgt
     2161 gctgtgggtt gaacaccggg acaaggggcg gctggaactg aattttctgg taccgaacac
     2221 ggaactgctg accggcaggc gtctccagcc gtactacgac cgggcagacc gtccgcgcat
     2281 cgatgcgtgg cagaccgtgg tgaacgggcg actggggctg cacgacccga acgcaccgga
     2341 gaaccggcgc gcgctggtga cgccatcttc gttacccaaa acgaagcagg aagccgcaga
     2401 ggctattacg cggggcttac tggccctcgc ctcgtcaggg gagcttaaaa cgcgtcagga
     2461 cgtcactgag gcgctggaaa gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat
     2521 cagcattgcc gacccggacg gagggcgaaa catccgactt aagggagcca tctatgaaca
     2581 gtcttttaac gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg
     2641 gcgaaatgct gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg
     2701 aaagcgggaa gagaatcagc gccgccatcc gcgcccacga gcagggtatg agcgcggcca
     2761 tgcagtcgaa ccgcctgagc gtgatgcgta tggtcgggcg gacatggctg accatcacga
     2821 tggtgtcagg actgctgttc gccagcctga gcggggtgtt gtggtatcag gggagcttga
     2881 tagcgtcaaa tctggccgaa atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga
     2941 agacctgggg cgtgacgtac ctggaagaca gcaacggacg ttttctggtg ctgccgaagg
     3001 ggacggccgt agacaggacg cagagctgga cggtcgggaa cgggcggtcg aagcagaacg
     3061 cgctgagact ggtgaaggag taagtgccga tgaccgagct ggaaaaacag ttgctgagcg
     3121 cattagagca gctacagcag gactactcga aaaggctgga cgagtgggag agcgccttcg
     3181 gggaatggcg gaaaatgtca gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg
     3241 tgacgcgctt gagtcagcag gtggaacgct tgagcgggca actgcagcgt ttgagccggt
     3301 agtgcagcgt catgagatgg ccgtggccgc agaacgggcg cacgagcaac ggcagcacga
     3361 aaaggaactg acggaagcgc gttcccggca gcgaagctat gacggtccgt cgctgggctg
     3421 aaatgcagga aaacagggtt ctcatcgggc atttacgctg aaaccggatg tcattcacct
     3481 ggctgcatgg ctatgcagcc aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg
     3541 tggtttgttg ccggttttac ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg
     3601 tgcgggcaac gggtgttatg tttaatcagc gtgaacggca gatacaaaaa agccccggag
     3661 ggcttttaac tactgactta tttttttgaa gatttgctgg gtttcaattt gtcagtattt
     3721 cccggcgttt ttttattgtc acgctggcgt ttatcaggtt cccctgttga atcggcaatt
     3781 cgtttcggac ccggcttaag acccataatc gttatccttt caatagttaa gaggaaagcc
     3841 ctcaagctag atatattgac cgcgttacgg attatcaaga ggggctaaca agattatttt
     3901 ttgttctttt aggttgattg tggtactgtt gatatacatg tatagcaaat ggtgttcggg
     3961 aggtttatat gcttgctatc cggttatctg atgagattga gtcccgtctg gactcgctgg
     4021 cgaagcaaac cggcagaaca aagacgtttt atgcgcggga agcaatactg gcgcatctgg
     4081 aagacctgga ggattattat ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg
     4141 aagcagtgca ttcgtctgaa gacgtgagga agtcacttgg tctggacgat taactattcc
     4201 gatcgggcgc tcaaatcgtt acgcaagatg gacaaacaga acgcacgacg gattgtggat
     4261 tttatgagtt tacgcattgc agttgctgcc gatcctcgcc agtcagggaa gccgctcaaa
     4321 ggtgagctgg gcgagttctg gcgctatcgg gtgggagatt atcgcgttct gtgtgagatc
     4381 cgagatgacg agcttgttat ccttgccgcc acgattggac atcgccgcga agtttacgac
     4441 tgaagcccgc tatgcgggct tttttgatgc cgacaacagg tattatggta aatactcgac
     4501 taactacgtg ttctactgtt tgtcggtttt agttaaccag caggttgtct taacgataat
     4561 gtagccgtta tttttaacta ataaggtcat attgttaacg tcaggcataa taaaagacat
     4621 tatggcctca gagttatcat cagaagcagc tctctgcatt actacttcga ttcccttttc
     4681 tttgaatcta taagtatcca gttgcctatt aaacttagaa agacctacaa ccatacttag
     4741 tttgttatct ttaacagtaa gagatgcttc ctgagtacca ttacatcggt agtaagcggt
     4801 gtaaccgtca gcaaaagatc gaccggggaa tataaatgct aggaacgcaa ttaaccaata
     4861 catagttgag aatcttggta ttacacattt cactatcctc tctttcattt ttcgttcacc
     4921 atactttgtt atctgatttt taatttttct attttataaa ccaagtctct accaaaaagg
     4981 cgaaaattat cctcaaataa taataattgt ttaagaacat ccatgaaagc agctgcttca
     5041 ccctcagtta tgggagctac tctatataac atataacctg agttaagggt ttcttttata
     5101 ctttcttcaa tgctcattat aaatattagt ggattatcat ttttcttccc ccatgggttt
     5161 ctaattaatc cttcgatgta ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt
     5221 atttcatctg cgtgagggct ttgttgtaca gctttgttta tgcctgcttg agtgattttc
     5281 agagactcta caccgtgtat gttgtatgag ataaaatgta ataaattaag atgcaatgca
     5341 attaatccat ttttgtttac atctgagtat ttggataaaa aacgtaatga agtgttaact
     5401 ctcccttgaa gcctaacaat tgggagtgct gccttgtata tttccttctc cgttaaatac
     5461 cgtttaaaaa acataattct ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa
     5521 cttagctctg aaatattcct ttagaaccat catgtcatta ataattgaat ctatctcatc
     5581 cattttttct tggtat
//
