LOCUS       NZ_CP050358            10060 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ZRKP01 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP050358
VERSION     NZ_CP050358.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14411773
            Assembly: GCF_015239295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     In Vivo Selection of Meropenem Resistance among Ceftazidime-
            Avibactam-Resistant, Meropenem- Susceptible Klebsiella pneumoniae
            Isolate with KPC-33 Carbapenemase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Bioinformatics center, GensKey Gene
            Technology Co. Ltd., Life Science Park Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-DEC-2019
            Assembly Method                   :: Unicycler v. version 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 13:57:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,905
            CDSs (total)                      :: 5,782
            Genes (coding)                    :: 5,628
            CDSs (with protein)               :: 5,628
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 49 of 154
            Pseudo Genes (incomplete)         :: 108 of 154
            Pseudo Genes (internal stop)      :: 19 of 154
            Pseudo Genes (multiple problems)  :: 18 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050358.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZRKP01"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China"
                     /lat_lon="39.98 N 116.43 E"
                     /collection_date="2019-10-14"
                     /collected_by="Laboratory of Clinical Microbiology and
                     Infectious Diseases, Department of Pulmonary and Critical
                     Care Medicine, China-Japan Friendship Hospital, Beijing, P.
                     R. China"
     gene            complement(join(9948..10060,1..259))
                     /gene="tolA"
                     /locus_tag="HB786_RS29200"
                     /old_locus_tag="HB786_29995"
     CDS             complement(join(9948..10060,1..259))
                     /gene="tolA"
                     /locus_tag="HB786_RS29200"
                     /old_locus_tag="HB786_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(533..778)
                     /locus_tag="HB786_RS29205"
                     /old_locus_tag="HB786_30000"
     CDS             complement(533..778)
                     /locus_tag="HB786_RS29205"
                     /old_locus_tag="HB786_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1105..2790
                     /locus_tag="HB786_RS29210"
                     /old_locus_tag="HB786_30005"
     CDS             1105..2790
                     /locus_tag="HB786_RS29210"
                     /old_locus_tag="HB786_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2800..3057
                     /locus_tag="HB786_RS29215"
                     /old_locus_tag="HB786_30010"
     CDS             2800..3057
                     /locus_tag="HB786_RS29215"
                     /old_locus_tag="HB786_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3142..3291
                     /locus_tag="HB786_RS29220"
                     /old_locus_tag="HB786_30015"
     CDS             3142..3291
                     /locus_tag="HB786_RS29220"
                     /old_locus_tag="HB786_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4775..6739
                     /locus_tag="HB786_RS29225"
                     /old_locus_tag="HB786_30020"
     CDS             4775..6739
                     /locus_tag="HB786_RS29225"
                     /old_locus_tag="HB786_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6739..7470
                     /gene="mobC"
                     /locus_tag="HB786_RS29230"
                     /old_locus_tag="HB786_30025"
     CDS             6739..7470
                     /gene="mobC"
                     /locus_tag="HB786_RS29230"
                     /old_locus_tag="HB786_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7477..8007
                     /locus_tag="HB786_RS29235"
                     /old_locus_tag="HB786_30030"
     CDS             7477..8007
                     /locus_tag="HB786_RS29235"
                     /old_locus_tag="HB786_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8034..8213)
                     /locus_tag="HB786_RS29240"
                     /old_locus_tag="HB786_30035"
     CDS             complement(8034..8213)
                     /locus_tag="HB786_RS29240"
                     /old_locus_tag="HB786_30035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8239..8667)
                     /locus_tag="HB786_RS29245"
                     /old_locus_tag="HB786_30040"
     CDS             complement(8239..8667)
                     /locus_tag="HB786_RS29245"
                     /old_locus_tag="HB786_30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8831..9145)
                     /locus_tag="HB786_RS29250"
                     /old_locus_tag="HB786_30045"
     CDS             complement(8831..9145)
                     /locus_tag="HB786_RS29250"
                     /old_locus_tag="HB786_30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9133..9411)
                     /locus_tag="HB786_RS29255"
                     /old_locus_tag="HB786_30050"
     CDS             complement(9133..9411)
                     /locus_tag="HB786_RS29255"
                     /old_locus_tag="HB786_30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9586..9951)
                     /locus_tag="HB786_RS29625"
     CDS             complement(9586..9951)
                     /locus_tag="HB786_RS29625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1..7790
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3395..3973)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6740..7467
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg gatatccgca
       61 cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca acagcggtct
      121 tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa attttcggcg
      181 gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat gccagcagga
      241 taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta catgtatatt
      301 tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc gtttttacac
      361 caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg
      421 cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg
      481 ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc
      541 ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc cgacgcattt
      601 cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt tcccgcacct
      661 gttgttttac cgcctttggc agcgttttgt tccctttcag gcccatggcc agtgcctgag
      721 cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg tgcgtcatag
      781 tggcctccgg gcgtctcagg tttcccgaac agtttaaccg caggtcctct cctcatgcca
      841 gttagctttc agatgtgtct ataccatctg aaatgtaagt gtgatgaatg tcttaaataa
      901 taaaatttgt tgccatgaga aattttcggg aaaagacgcg atctgcttac aggtggctcc
      961 gggcgctttt ttgtgcctga agatgagaac taatctcata tattgacacc tgaaaacggg
     1021 aggagtaagg taataattat actgtgtata tgtacagtat gttggcttta atttattgtt
     1081 ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg gtcgaggtcc gggtaattca
     1141 ggtctgggac ataatggtgg tcaggccagt gggaatgtga acggtacgtc tggtaaaggt
     1201 ggcccttcat caggtggtgg tacggatcca aacagcgggc cgggctgggg tacgacgcat
     1261 acgccgaatg gagatattca taactataat ccgggggagt ttggtaacgg cgggagtaaa
     1321 cccggtggta atggtggtaa cagcggcaat catagcggta gctctggtgg cggacagtct
     1381 tcggccactg cgatggcctt cggtctgcct gccctggcta ctccgggggc tgaaggactg
     1441 gctttatcag tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct ggctgccctg
     1501 aaggggccgt ttaagtttgg tctgtggggg attgcgatct acggtgtgct gccttctgag
     1561 attgcaaaag atgatccgaa aatgatgtca aaaattatga cgtcattacc ggccgatgcg
     1621 gtgacggaga ctccggcaag tactttacca ctggaccagg cgacggttcg tgtcagacaa
     1681 cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg cggttgtcgc aggtcggcca
     1741 atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg
     1801 attccgggtc tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac agcgaaagcc
     1861 ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc cggctggttt tacagccggt
     1921 ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa gccggtttat
     1981 gtgtcggtga cagatgttct taccccggca caggtaaaac agcgtcagga ggaagaaaag
     2041 cgtcgccagc aggcatggga cgccgctcat ccggaagagg ggctgaaaag agactatgat
     2101 aaagcgaaag ctgagctgga tgccgaagat aaaaatattg cgaccttaaa cagccgcatt
     2161 gcatcgacag agaaggcgct ccccggtgca agggctgctg ttcaggaagc cgataaaaag
     2221 gtaaaagagg cagaggcaaa taaagatgat tttgtgactt ataacccgcc tcatgaatat
     2281 ggctccgggt ggcaggatca ggttcgctat cttgataagg atattcagaa tcagaatgag
     2341 aaattaaaag cggctcaggc atctttaaac gcaatgaatg aatccttatc cagagataag
     2401 gctgcactta ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca
     2461 gaaaataagt taaatgagga aaagaacaag cctcgcaagg ggactaaaga ctacggccat
     2521 gattatcatc cagtacctaa aacagaagac ataaagggac ttggagagtt aaagcgaggt
     2581 gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg cccgatggta tggcgataaa
     2641 aaacgtaaaa tttatgaatg ggattcccag cacggtgagc ttgaagggta tcgtgccagt
     2701 gatggcgaac acctaggcgc atttgatccc aaaacgggta agcaggttaa agggccagat
     2761 ccaaaacgga acattaaaaa atatctttga gaggtaaata tggggcttaa attacgtttg
     2821 acatggtttg ataagaaaac tgaagagttt aaaggggaag agtattctaa agactttggt
     2881 gacgatggtt ctgtcattga aagcttggga atgcctttaa aggataatat taataatggt
     2941 gagtttgatt tgaagaaaga atgggttcct ttactacagc cattttttaa aaatcaaata
     3001 aaacctgatg tggataattg ttatatagct tttgattatc gggacggtaa ctggtaacaa
     3061 aaaattatca aggatgattt gaaatactga cttccggtgt tcagggatga atgccggagt
     3121 ctttaaatac agggaaagta tatgaaaaag gtaaagacta tttttctttt tatattgatt
     3181 gttgctggct ttcttctggc cgcatgtcag gcaaattata tccgggatgt tcagggcgga
     3241 acggtggcac catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca
     3301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa
     3361 ccacctgggg aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt
     3421 aactggcttt cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc
     3481 gcacacttca agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac
     3541 caatggctgc tgccagtggc gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt
     3601 accggataag gcgcagcagt cgggctgaac ggggagttct tgcttacagc ccagcttgga
     3661 gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc tatgagaaag cgccacactt
     3721 cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc agggtcggaa caggagagcg
     3781 caagagggag cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg
     3841 tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt
     3901 ttatgtgaac tctggcagga gggcggagcc tatggaaaaa cgccaccggc gcggccctgc
     3961 cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg
     4021 tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg
     4081 cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc
     4141 ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt
     4201 tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg
     4261 caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct
     4321 ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc
     4381 cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac
     4441 ggtgctggac gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg
     4501 ctcgctatgg cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg
     4561 ccttttcctc tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc
     4621 atggggcgga aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga
     4681 ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg
     4741 ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg
     4801 gcagtggcat atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg
     4861 ggagtattac agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga
     4921 actctgggcc ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc
     4981 cgatcgctat aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga
     5041 ttcacccggg cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac
     5101 cggcgctgcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg
     5161 gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc
     5221 ggctaacctg ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc
     5281 gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa
     5341 cgcgaaactg aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt
     5401 ccgccacctg catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga
     5461 gcaggcccgg aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct
     5521 ggtccctcgt gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg
     5581 caagagcgcg atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt
     5641 ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc
     5701 ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga
     5761 tctcgaccag tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg
     5821 cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca
     5881 gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc
     5941 cctgaaaaag gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt
     6001 cctgcagacg gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta
     6061 cgtcatcggc tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg
     6121 ctgcgcgcaa atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat
     6181 gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg
     6241 cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg
     6301 gcaggatctg cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg
     6361 gttctatcgc gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat
     6421 ccgggtggac gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag
     6481 cggcgacagc ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca
     6541 cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag
     6601 cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac
     6661 ggacccgctg gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga
     6721 ttttgcgggg ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag
     6781 gcgcggaacg cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc
     6841 acggcggagc tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc
     6901 ctggacagtc tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt
     6961 caggtcagcg tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag
     7021 ccgctgacgg acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac
     7081 cggctggccg tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg
     7141 tacctgcacc gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac
     7201 ggcagaacgg tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa
     7261 gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc
     7321 tgccctgacc ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc
     7381 gtgaatccac agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc
     7441 gcgtgcttta aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt
     7501 gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc caaccagcga gcatctggaa
     7561 gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc
     7621 cagcatctgg cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa
     7681 aaagccacgt actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga
     7741 atgaagctgt gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg
     7801 caggtggttg atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc
     7861 agcgagcggc acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa
     7921 tataacagcc gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc
     7981 cggtggtggc cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct
     8041 tccccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg
     8101 cacatgtcgg ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac
     8161 tgatccttaa tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc
     8221 attcccttcg gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt
     8281 cagttctgtt gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg
     8341 gcggcgttct tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag
     8401 atcggccagt tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt
     8461 ttcacggtgt gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg
     8521 gatcagttct gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt
     8581 tccctgtttt acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg
     8641 tgaaaggttg agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt
     8701 gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg
     8761 gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg
     8821 tcatcggtta ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg
     8881 tgacgatcac aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt
     8941 cgccaaaccc gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa
     9001 tgtctggcaa tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg
     9061 ctgccagccg atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca
     9121 gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt
     9181 acttatccag gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg
     9241 ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc
     9301 ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt
     9361 cagccggtcc ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc
     9421 tttgtagtca tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa
     9481 atacggcttt tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct
     9541 cactcatcat ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc
     9601 atcatcattg agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag
     9661 actgaattca aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg
     9721 cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc
     9781 cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc
     9841 tgcggcctgt accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg
     9901 taccggagtg aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa
     9961 tccagagggg cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg
    10021 gcgtgggtaa cggctgatat tgctgcttta cagagtttcg
//
