LOCUS       NZ_CP050353             5596 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ZRKP02 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP050353
VERSION     NZ_CP050353.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14411774
            Assembly: GCF_015239135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     In Vivo Selection of Meropenem Resistance among Ceftazidime-
            Avibactam-Resistant, Meropenem- Susceptible Klebsiella pneumoniae
            Isolate with KPC-33 Carbapenemase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Bioinformatics center, GensKey Gene
            Technology Co. Ltd., Life Science Park Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-DEC-2019
            Assembly Method                   :: Unicycler v. version 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 92x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 16:04:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,892
            CDSs (total)                      :: 5,769
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 53 of 158
            Pseudo Genes (incomplete)         :: 108 of 158
            Pseudo Genes (internal stop)      :: 20 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050353.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZRKP02"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China"
                     /lat_lon="39.98 N 116.43 E"
                     /collection_date="2019-10-29"
                     /collected_by="Laboratory of Clinical Microbiology and
                     Infectious Diseases, Department of Pulmonary and Critical
                     Care Medicine, China-Japan Friendship Hospital, Beijing, P.
                     R. China"
     gene            45..269
                     /locus_tag="HB787_RS29195"
                     /old_locus_tag="HB787_29990"
     CDS             45..269
                     /locus_tag="HB787_RS29195"
                     /old_locus_tag="HB787_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            253..519
                     /locus_tag="HB787_RS29200"
                     /old_locus_tag="HB787_29995"
     CDS             253..519
                     /locus_tag="HB787_RS29200"
                     /old_locus_tag="HB787_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(589..939)
                     /locus_tag="HB787_RS29205"
                     /old_locus_tag="HB787_30000"
     CDS             complement(589..939)
                     /locus_tag="HB787_RS29205"
                     /old_locus_tag="HB787_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1005..1550)
                     /locus_tag="HB787_RS29210"
                     /old_locus_tag="HB787_30005"
     CDS             complement(1005..1550)
                     /locus_tag="HB787_RS29210"
                     /old_locus_tag="HB787_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(1576..1902)
                     /locus_tag="HB787_RS29215"
                     /old_locus_tag="HB787_30010"
     CDS             complement(1576..1902)
                     /locus_tag="HB787_RS29215"
                     /old_locus_tag="HB787_30010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2260..2418)
                     /locus_tag="HB787_RS29755"
     CDS             complement(2260..2418)
                     /locus_tag="HB787_RS29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            3058..3234
                     /locus_tag="HB787_RS29220"
                     /old_locus_tag="HB787_30015"
     CDS             3058..3234
                     /locus_tag="HB787_RS29220"
                     /old_locus_tag="HB787_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3224..3568
                     /locus_tag="HB787_RS29225"
                     /old_locus_tag="HB787_30020"
     CDS             3224..3568
                     /locus_tag="HB787_RS29225"
                     /old_locus_tag="HB787_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3777..>4211
                     /locus_tag="HB787_RS29650"
                     /pseudo=""
     CDS             3777..>4211
                     /locus_tag="HB787_RS29650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4246..4755
                     /locus_tag="HB787_RS29235"
                     /old_locus_tag="HB787_30030"
     CDS             4246..4755
                     /locus_tag="HB787_RS29235"
                     /old_locus_tag="HB787_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4762..4974
                     /locus_tag="HB787_RS29240"
                     /old_locus_tag="HB787_30035"
     CDS             4762..4974
                     /locus_tag="HB787_RS29240"
                     /old_locus_tag="HB787_30035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2893..2972
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2310..2707)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3559..5090
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actgttgata tacatgtata gcaaatggtg ttcgggaggt ttatatgctt gctatccggt
       61 tatctgatga gattgagtcc cgtctggact cgctggcgaa gcaaaccggc agaacaaaga
      121 cgttttatgc gcgggaagca atactggcgc atctggaaga cctggaggat tattatcttt
      181 cagcagaaac tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg tctgaagacg
      241 tgaggaagtc acttggtctg gacgattaac tattccgatc gggcgctcaa atcgttacgc
      301 aagatggaca aacagaacgc acgacggatt gtggatttta tgagtttacg cattgcagtt
      361 gctgccgatc ctcgccagtc agggaagccg ctcaaaggtg agctgggcga gttctggcgc
      421 tatcgggtgg gagattatcg cgttctgtgt gagatccgag atgacgagct tgttatcctt
      481 gccgccacga ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg cgggcttttt
      541 tgatgccgac aacaggtatt atggtaaata ctcgactaac tacgtgttct actgtttgtc
      601 ggttttagtt aaccagcagg ttgtcttaac gataatgtag ccgttatttt taactaataa
      661 ggtcatattg ttaacgtcag gcataataaa agacattatg gcctcagagt tatcatcaga
      721 agcagctctc tgcattacta cttcgattcc cttttctttg aatctataag tatccagttg
      781 cctattaaac ttagaaagac ctacaaccat acttagtttg ttatctttaa cagtaagaga
      841 tgcttcctga gtaccattac atcggtagta agcggtgtaa ccgtcagcaa aagatcgacc
      901 ggggaatata aatgctagga acgcaattaa ccaatacata gttgagaatc ttggtattac
      961 acatttcact atcctctctt tcatttttcg ttcaccatac tttgttatct gatttttaat
     1021 ttttctattt tataaaccaa gtctctacca aaaaggcgaa aattatcctc aaataataat
     1081 aattgtttaa gaacatccat gaaagcagct gcttcaccct cagttatggg agctactcta
     1141 tataacatat aacctgagtt aagggtttct tttatacttt cttcaatgct cattataaat
     1201 attagtggat tatcattttt cttcccccat gggtttctaa ttaatccttc gatgtactca
     1261 ggtcctttgc gtcctattgc ctgcgcaata ttttttattt catctgcgtg agggctttgt
     1321 tgtacagctt tgtttatgcc tgcttgagtg attttcagag actctacacc gtgtatgttg
     1381 tatgagataa aatgtaataa attaagatgc aatgcaatta atccattttt gtttacatct
     1441 gagtatttgg ataaaaaacg taatgaagtg ttaactctcc cttgaagcct aacaattggg
     1501 agtgctgcct tgtatatttc cttctccgtt aaataccgtt taaaaaacat aattctccgc
     1561 ctccgtatag cgtgcctatt ttatttcgtc gtaaaactta gctctgaaat attcctttag
     1621 aaccatcatg tcattaataa ttgaatctat ctcatccatt ttttcttggt attcttttaa
     1681 tggtgaaggg agcggtaact caatatttga aaagtgattg gcgagatagg gatcaaggtt
     1741 tctcatccat tcaggctttt cgccaatgcg ttcttcaact gaaagtgaac ctgataatct
     1801 ctggttaata agatcactta gactcatttt ggttttattc gaaagccgtt gtaaggattg
     1861 ctttaatcct atatctagca tgatatttgt tctttctctc attacccccc cttgctattg
     1921 cgttttttgt tgcgcacaaa gctaccattg cgcattaaga aacgcaataa aaaaggaggg
     1981 agggagtggt ttgtacatga tgtgcagtgc gctgagggca tcagtggcgg gtgtttttcc
     2041 gtgggatgga tggagctccg gggataacag aactgacgat catttaaagg atcttcttga
     2101 gatccttttt ttctgcgcgt aatctcttgc actgtaaacg aaaaaaccgc ctggggaggc
     2161 ggtttgatcg aaggttaagt cagttgggga actgcttaac cgggtaactg ggtatacgag
     2221 accgcagata ccaaaactgt tctttcagtg tagccgcagt taggcaccca cttcaagacc
     2281 tctcaatatc tctctcgtat atatccagtt accaatggct gctgccagtg gcgtttttgc
     2341 gtgtccgtcc gggttggact caagatgata gttaccggaa agggcgcagc agtcgggctg
     2401 aacggggggt tcgtgcatac agcccagctt ggagcgaacg acctaaaccg agccgagata
     2461 ccagcagcgt gagctatgag aaagcgccac gcttcccgaa agggaaaaag gcggaacagg
     2521 tatccggtaa acggcagggt cggaacagga gagcgcacga gggagccgcc ggagagaaat
     2581 caccggcatc tttaagtcct gtcgggtttc gtcctcctct gacttgagcg tccatttctg
     2641 tgatgctcgt caggggaggc ggagcctatg gaaaaacggc aacgccgcat ccttttctcc
     2701 ggccctttct gatttgagcg cctttgcttt atcccgttat ccgtgagtcg gtcacgcccg
     2761 ccgcagccga acggcaggag cgcagctacc gagtgagcga ggaggcgtca tttcatctgt
     2821 ccgtgcatgg tattgcggac gctcctgagc cacatatcac acctgccaca tgaggcactt
     2881 tcatgaatcg tgctctcatg ccaacatagt aagccagtat acacagcgta aacgctgtga
     2941 ctggttcagg gctgcgcccc gaaacccgct aacaccactg acgcgctggc gcttgtccaa
     3001 cgccggagcg acccggaaaa cttttccgtg ccgtccggcg tttgcgaagg agggataatg
     3061 cagaagccga agaagaagcc gctcgtcgta aaaaaaagat cctggcatcc ggcagtgatg
     3121 gcaaatctga tgcgtcaggc acaggaggaa gattaccgcc gggagcagat ggcagagctt
     3181 gagcgttcag cccggtcaca ttttgcggat atcggaggag aggatggcgg ttaagcgtac
     3241 ccgtttttta ggtatccggg tgaccgatgg ggagtaccag cagctgctgg agcgctgcaa
     3301 tggaaggcaa ctggcggtgt ggatgcggga aacctgtctc gatacgcgcc cggcgcggtc
     3361 gttgcggctg ccgtccatcg atccggtttt gctgcgccag cttgccggga tggggaacaa
     3421 cctcaaccag atcgcccgga aaattaacgg cggccagtgg tcaggcgctg accgggttca
     3481 ggtggtggcc gcgctgatgg ccattgatgc cgaactcgag cggctgcggc atgccgtgct
     3541 ggaaaagggg gcagacgatg atcgttaagt ttcatccgcg agggcgaggc ggcggtgccg
     3601 ggccggtgga ttatctgctg gggaaagacc gccagcgcga cggggccatc gtcctgcagg
     3661 gaaagccgga ggaggtccgg gagcttatcg acgcctcgcc ctacgccaaa aagtacacct
     3721 ccggcgtcct gtcttttgct gaacaggatt taccgcccgg ccagcgtgaa aagctgatgg
     3781 cgagcttcga gcgggttctg atgcccggac tcgataaaga ccagtacagc gtgctgtggg
     3841 ttgaacaccg ggacaagggg cggctggaac tgaattttct ggtaccgaac acggaactgc
     3901 tgaccggcag gcgtctccag ccgtactacg accgggcaga ccgtccgcgc atcgatgcgt
     3961 ggcagaccgt ggtgaacggg cgactggggc tgcacgaccc gaacgcaccg gagaaccggc
     4021 gcgcgctggt gacgccatct tcgttaccca aaacgaagca ggaagccgca gaggctatta
     4081 cgcggggctt actggccctc gcctcgtcag gggagcttaa aacgcgtcag gacgtcactg
     4141 aggcgctgga aagcgcaggt tttgaggtcg tgcgcaccac aaagagcagc atcagcattg
     4201 ccgacccgga cggagggcga aacatccgac ttaagggagc catctatgaa cagtctttta
     4261 acgctggcga aggacttaga gcagaaatcg aaagcgcagc agcagagtac cggcgaaatg
     4321 ctgaaagccg cattcagcga gcacgagaag tctgtcagag cggaactgag cgaaagcggg
     4381 aagagaatca gcgccgccat ccgcgcccac gagcagggta tgagcgcggc catgcagtcg
     4441 aaccgcctga gcgtgatgcg tatggtcggg cggacatggc tgaccatcac gatggtgtca
     4501 ggactgctgt tcgccagcct gagcggggtg ttgtggtatc aggggagctt gatagcgtca
     4561 aatctggccg aaatcgaccg gcagaacgca gcgctgtcga agctgaacgc gaagacctgg
     4621 ggcgtgacgt acctggaaga cagcaacgga cgttttctgg tgctgccgaa ggggacggcc
     4681 gtagacagga cgcagagctg gacggtcggg aacgggcggt cgaagcagaa cgcgctgaga
     4741 ctggtgaagg agtaagtgcc gatgaccgag ctggaaaaac agttgctgag cgcattagag
     4801 cagctacagc aggactactc gaaaaggctg gacgagtggg agagcgcctt cggggaatgg
     4861 cggaaaatgt cagggcttat gcaacgggag aacgcggcgc tgagcgagcg cgtgacgcgc
     4921 ttgagtcagc aggtggaacg cttgagcggg caactgcagc gtttgagccg gtagtgcagc
     4981 gtcatgagat ggccgtggcc gcagaacggg cgcacgagca acggcagcac gaaaaggaac
     5041 tgacggaagc gcgttcccgg cagcgaagct atgacggtcc gtcgctgggc tgaaatgcag
     5101 gaaaacaggg ttctcatcgg gcatttacgc tgaaaccgga tgtcattcac ctggctgcat
     5161 ggctatgcag ccaggtaaaa tttatctgtc gcgaacgggg cggtttatcg ggtggtttgt
     5221 tgccggtttt acctgactgc caccccgttc gcggcgaacc cgtccggggc ggtgcgggca
     5281 acgggtgtta tgtttaatca gcgtgaacgg cagatacaaa aaagccccgg agggctttta
     5341 actactgact tatttttttg aagatttgct gggtttcaat ttgtcagtat ttcccggcgt
     5401 ttttttattg tcacgctggc gtttatcagg ttcccctgtt gaatcggcaa ttcgtttcgg
     5461 acccggctta agacccataa tcgttatcct ttcaatagtt aagaggaaag ccctcaagct
     5521 agatatattg accgcgttac ggattatcaa gaggggctaa caagattatt ttttgttctt
     5581 ttaggttgat tgtggt
//
