LOCUS       NZ_CP050352            10060 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ZRKP02 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP050352
VERSION     NZ_CP050352.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14411774
            Assembly: GCF_015239135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     In Vivo Selection of Meropenem Resistance among Ceftazidime-
            Avibactam-Resistant, Meropenem- Susceptible Klebsiella pneumoniae
            Isolate with KPC-33 Carbapenemase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Bioinformatics center, GensKey Gene
            Technology Co. Ltd., Life Science Park Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-DEC-2019
            Assembly Method                   :: Unicycler v. version 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 92x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 16:04:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,892
            CDSs (total)                      :: 5,769
            Genes (coding)                    :: 5,611
            CDSs (with protein)               :: 5,611
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 53 of 158
            Pseudo Genes (incomplete)         :: 108 of 158
            Pseudo Genes (internal stop)      :: 20 of 158
            Pseudo Genes (multiple problems)  :: 19 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050352.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZRKP02"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China"
                     /lat_lon="39.98 N 116.43 E"
                     /collection_date="2019-10-29"
                     /collected_by="Laboratory of Clinical Microbiology and
                     Infectious Diseases, Department of Pulmonary and Critical
                     Care Medicine, China-Japan Friendship Hospital, Beijing, P.
                     R. China"
     gene            complement(join(9247..10060,1..872))
                     /locus_tag="HB787_RS29130"
                     /old_locus_tag="HB787_29925"
     CDS             complement(join(9247..10060,1..872))
                     /locus_tag="HB787_RS29130"
                     /old_locus_tag="HB787_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1199..1444
                     /locus_tag="HB787_RS29135"
                     /old_locus_tag="HB787_29930"
     CDS             1199..1444
                     /locus_tag="HB787_RS29135"
                     /old_locus_tag="HB787_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1718..2089
                     /gene="tolA"
                     /locus_tag="HB787_RS29140"
                     /old_locus_tag="HB787_29935"
     CDS             1718..2089
                     /gene="tolA"
                     /locus_tag="HB787_RS29140"
                     /old_locus_tag="HB787_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            2086..2451
                     /locus_tag="HB787_RS29550"
     CDS             2086..2451
                     /locus_tag="HB787_RS29550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            2626..2904
                     /locus_tag="HB787_RS29150"
                     /old_locus_tag="HB787_29945"
     CDS             2626..2904
                     /locus_tag="HB787_RS29150"
                     /old_locus_tag="HB787_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2892..3206
                     /locus_tag="HB787_RS29155"
                     /old_locus_tag="HB787_29950"
     CDS             2892..3206
                     /locus_tag="HB787_RS29155"
                     /old_locus_tag="HB787_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3370..3798
                     /locus_tag="HB787_RS29160"
                     /old_locus_tag="HB787_29955"
     CDS             3370..3798
                     /locus_tag="HB787_RS29160"
                     /old_locus_tag="HB787_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3824..4003
                     /locus_tag="HB787_RS29165"
                     /old_locus_tag="HB787_29960"
     CDS             3824..4003
                     /locus_tag="HB787_RS29165"
                     /old_locus_tag="HB787_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4030..4560)
                     /locus_tag="HB787_RS29170"
                     /old_locus_tag="HB787_29965"
     CDS             complement(4030..4560)
                     /locus_tag="HB787_RS29170"
                     /old_locus_tag="HB787_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4567..5298)
                     /gene="mobC"
                     /locus_tag="HB787_RS29175"
                     /old_locus_tag="HB787_29970"
     CDS             complement(4567..5298)
                     /gene="mobC"
                     /locus_tag="HB787_RS29175"
                     /old_locus_tag="HB787_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(5298..7262)
                     /locus_tag="HB787_RS29180"
                     /old_locus_tag="HB787_29975"
     CDS             complement(5298..7262)
                     /locus_tag="HB787_RS29180"
                     /old_locus_tag="HB787_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8746..8895)
                     /locus_tag="HB787_RS29185"
                     /old_locus_tag="HB787_29980"
     CDS             complement(8746..8895)
                     /locus_tag="HB787_RS29185"
                     /old_locus_tag="HB787_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8980..9237)
                     /locus_tag="HB787_RS29190"
                     /old_locus_tag="HB787_29985"
     CDS             complement(8980..9237)
                     /locus_tag="HB787_RS29190"
                     /old_locus_tag="HB787_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             1..5872
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          8065..8643
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4571..5298)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 accggcttct gtccggtctc tttcgggaaa cgaataacgg cctcacggga gttaccaccg
       61 gctgtaaaac cagccggacg tgaatcacct ttttcagcaa caatgccttt tggcggggct
      121 ttcgctgtcg gaacaccttt cggtacgctc acctgcagag acgggagacc cggaatcgac
      181 acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacagggac actcattggc
      241 cgacctgcga caaccgcaat atgctgccgc tcatccttca ccacatccac aacccgttgt
      301 ctgacacgaa ccgtcgcctg gtccagtggt aaagtacttg ccggagtctc cgtcaccgca
      361 tcggccggta atgacgtcat aatttttgac atcattttcg gatcatcttt tgcaatctca
      421 gaaggcagca caccgtagat cgcaatcccc cacagaccaa acttaaacgg ccccttcagg
      481 gcagccagca catcagcaac ggcggccgac aacgcatcgc cggaaactga taaagccagt
      541 ccttcagccc ccggagtagc cagggcaggc agaccgaagg ccatcgcagt ggccgaagac
      601 tgtccgccac cagagctacc gctatgattg ccgctgttac caccattacc accgggttta
      661 ctcccgccgt taccaaactc ccccggatta tagttatgaa tatctccatt cggcgtatgc
      721 gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga agggccacct
      781 ttaccagacg taccgttcac attcccactg gcctgaccac cattatgtcc cagacctgaa
      841 ttacccggac ctcgaccgtc tccaccactc ataccgtttt cctctttgac atttaaaaca
      901 ataaattaaa gccaacatac tgtacatata cacagtataa ttattacctt actcctcccg
      961 ttttcaggtg tcaatatatg agattagttc tcatcttcag gcacaaaaaa gcgcccggag
     1021 ccacctgtaa gcagatcgcg tcttttcccg aaaatttctc atggcaacaa attttattat
     1081 ttaagacatt catcacactt acatttcaga tggtatagac acatctgaaa gctaactggc
     1141 atgaggagag gacctgcggt taaactgttc gggaaacctg agacgcccgg aggccactat
     1201 gacgcacctg aaactgactc ccgctgaaga aaagcgcctg gcggacgcct ttgtcgctca
     1261 ggcactggcc atgggcctga aagggaacaa aacgctgcca aaggcggtaa aacaacaggt
     1321 gcgggaaagg gtgcttcgtg ccattcagga cgaacgtgaa gccaggcggg cgcaggaaat
     1381 gcgtcgggag aaatacgagg ctacggaaac caccttcacc tggcagccac agcgccgccg
     1441 gtaaggggta tttaccataa cgtcgcattg cccgcaccgc tcagacggtc tgagggcgtt
     1501 tccggcggct acgggataaa acaggcaaca aaccacccgg aaaaccgccg gaaacgcgcc
     1561 cggtaaattg taacccgctg catgcctatg cacgcaactg catggcgggc cgtttggtgt
     1621 aaaaacgata cgataagccg tgtttatgta aatgaacgtg gccgtaaaga gtgacaaata
     1681 tacatgtaac attttttact gacaggtcat caacccgatg cgtttaatac cctttatcct
     1741 gctggcattg tgcagcccct tactcctgac ggcctgtaag cattccacca cccagccgcc
     1801 gaaaatttat actctggata agtcatctaa aatcagtagc tacgctgcgg ccatcaagac
     1861 cgctgttgaa atgcagatgt ttgaaccaga ggcctatgcg ggaaaaatgt gcaccgtgcg
     1921 gatatccata caacgtgatg gcactctgaa cagcgccatt gctgaaggag gagatccgaa
     1981 actctgtaaa gcagcaatat cagccgttac ccacgccaaa attccgccgg caccggatga
     2041 aaatacctgg caaatattca aaaacgcccc tctggatttc agaccatgaa agcttttact
     2101 ctgacagtgc tggcggccat gttcctcact ccggtacatg cgataccact aaaacccgta
     2161 accacactgc agccaaccta cccggtacag gccgcagcaa aacgcatcga aggtcaggtg
     2221 accgccacgt ttgatattct ggacgatgga cgagtggaca acgtgtcgct taccggctgt
     2281 cacctttttt ttcgggaaac gctgacggca atgaggcact ggctatatcc cgcagggcac
     2341 ccggcaatgc gacaggttat aaattttgaa ttcagtctga aaaatgacgg cttacaggat
     2401 ccgcgaccag tcaccaccca aaagctcaat gatgatggtt gccgccagta acaggtcctt
     2461 taacccgcag gctgcaatag tcctggatga tgagtgagcc tgtagcagcc ctgattagtg
     2521 gaagcgaagc tgaaaaatgg gcagcaaaag ccgtatttct ggcaaatagc gccgccacgg
     2581 attaaagtag tcatgtgtaa tacatatgac tacaaagggg cttttatgtc atcagcaaca
     2641 tccatcaggc tcgatgaaga acttaaggac cggctgaaaa cgctggccga tgaccggcac
     2701 cgctcagcgc acgcgctaat gctggaggca atcaccgaat acatcgaccg ggaagagaag
     2761 cgcagccagt acctgcggga cggacaggcc gcatggcagc actatcagga aacgggtctg
     2821 caccttactg cggaagaagc ggaagcctgg ataagtacct ggggaactga gaacgagcag
     2881 gatgctccgc catgccacag gtaacgattt ccgcgctggc acagcgcgat ttacaacgcc
     2941 ttcaggactt tctcaaaacc aaaaatcggc tggcagccag aaaaggaggt gaggtgatcg
     3001 tccgggctat ccagcaactg aaaacattgc cagacattgg ccgcccggtg ccgtttctgc
     3061 cgctggaata taaggaactg gtgatcgggt ttggcgacag tggctatgtg atgctctacc
     3121 gccacgaccg ggaaatggac cagattgtga tcgtcaccgt caggcatcag aaagaatccg
     3181 ggtatccggg ggcagacagc ctctaataac cgatgacggg ggcgcaattc attgcgtggc
     3241 cgtcgccggt ttgacaagcg cagcgcgtca gaaaaccaca tcctgaacgg aaaaacgggt
     3301 aaaattcagc gatacattta ccgtgagaaa ttacacacct ttcacacccg ataaaatcag
     3361 gggtatacag tggcgaaaac attgctcaac ctttcacagg cagcacaagc cgcgggcatt
     3421 accagaagaa cgctatacaa tcatgtaaaa cagggaaagg ttaccgtgtc acgtgacggg
     3481 aaaaacaacc ctgtcgtgga tgtgtcagaa ctgatccgtg tatacggaaa tgtgaatatc
     3541 cctgagaaac aaattcccgg catttcacac cgtgaaaata cacaaaaaaa tttcccacag
     3601 gaacagcttc tggcaatgca aaaggaactg gccgatctcc ggcaggctgt caccctgatg
     3661 ctggaagata aaacatcccg ggaagaagaa cgccgccagc acgatgatga gcgccggaaa
     3721 ctccaggccg aagtggacag attgacaaca gaactgacac agaagaaaaa acgcttctgg
     3781 tccggctggt ttagctgaga acttaccgaa gggaatggag aacatgacga agcaggaaaa
     3841 aactgccctc aatatggcta aattcattaa ggatcagtcg ctgctgttgc tggaaaagct
     3901 gaacgagctg gacatggata ccccggccga catgtgcgaa cggcttcacg aggatgcgga
     3961 aacactctgg caggtgatgc gggataagct cggggaagaa taacagcacc ggccatgccg
     4021 gtgctggcgt tattttttgc cgaacggcca ccaccggcgg gcgggtttgt cctccggcag
     4081 ttcaggcacc ggcacgttaa aatagcggct gttatattcc cgcatcagct ccgctttcag
     4141 cttctcgttc accgcgcttt ccccgtgccg ctcgctgacc acctcaatca tcgcgttaat
     4201 ctgctccggt gagcacttcc cggcatcaac cacctgcaca ccgttctcga tggcgaaccg
     4261 ctccaccttt cgatagaggt aatcacacag cttcattcct gtgggtttgt cccgttccca
     4321 ttgcggattt tcaccggtga aaaagtacgt ggctttttcg tcgctgttga gccgctcgtc
     4381 atacatcccg atggatttca tcatcgccag atgctgggcc cggtcgacaa aacagaaaac
     4441 atacgccccg gtctgccagg cgagcaccac catgtcttcc agatgctcgc tggttggcat
     4501 aatgcagcgc tcgccgtctt tgtcttccac gtcctcaata aataccatgt acagcgccat
     4561 aatccctcac tccgttgtca taaatttaaa gcacgcgaac agcctctccc ggtccagctc
     4621 caccgttttg cgggcctcgc cggtctgtgg attcacgatt tcatccaccg ccagaatcgc
     4681 ccgctgtacc cggacaagcg tcggccggtc agggcagatg taccagattt catcgatgcc
     4741 gttcgcccgg cggttgaaca gatacccgga caccacttcc tgataccgcc tgcggctctt
     4801 gacggtgcgc tctacctcaa atgccaccgt tctgccgtcc agctccgcca gcgcgtccgg
     4861 cactttcatc cctttcaggg ccatacggtg caggtaccgc cagccggtgg ccccccggcc
     4921 ctccatggag aggcgcaggg actgcacggc cagccggtgc ggcagctgcg ccgccgacac
     4981 ccttgacggc tgaaacggaa taacgtccgt cagcggctcg tcctcgtcga acgagaacgc
     5041 caccccgtgc ggcgtcagcc cccacacgct gacctgacgg ccgtcaaccg ggtatttcgc
     5101 ccggaccagc agccctttcg ccaccagact gtccaggaag cggtaaaccg gctgtaccgc
     5161 cgcaatcccc agccgtcgcg caaccagctc cgccgtggaa cacagctctt cccgcagcca
     5221 gcgcagggtc agccgtgttt tctccgcgtt ccgcgcctgc cgcttcgcgt ggctgacgtt
     5281 gtactgctcc agtgtcatca gtaaagcccc gcaaaatcat catccgcttt tggtccgccg
     5341 ccgtcggccg gaggcaggta ctccgccagc gggtccgttt tcgccagcac cgggaaggtt
     5401 ttcagcgaaa tttcccggcg ctcaacccgc agcgggctgc tgaacgccag ccgcgccacg
     5461 cccagcccgg tcatcaccgc gaacccgtca ggcaggtgtt gcagggtgtt cacgtcaaac
     5521 agcggccgcg cctccttctg gatgaagctg tcgccgctga tgtgctccac gttccccgcc
     5581 tcccggctgg cccggcggcg ctccacgtcc acccggatct ccccggtctg gcccgccgcc
     5641 cactgcgcgg actcctggct ggccgcgcga tagaaccagc ggatcggcgt gttatccagt
     5701 accgtggttt tcacaaaatc agcgggcaga tcctgcccgc actgaccgaa gtcgcccagc
     5761 gactggtgcg cgagcagcag gttgcagtcc cggctgcgca gcgtccccag cgcgttcagc
     5821 acgtacttcg acagcagata tttaatctca tcgagcatga tgctcgcgtg cggccacctc
     5881 cggaactcat cccgcgcaat gatgatttgc gcgcagcggg caaacagcat cttctgcacc
     5941 cggatcaccg cctcatcgtc catcgagccg atgacgtaca ggcagccgcc gccgttgatt
     6001 atccccgcca catcgatacc gctgtccgtc tgcaggacgc tcaggtcggc cacgttctcc
     6061 agcttggtga tcagcccctt cacgtccttt ttcagggcct ccggcaacgc gtacgccgcc
     6121 gccagcacct gctgaatgtt tgccccctgc gggaactgct ccgcaatcag cttcgccgct
     6181 ttctgctcgc tgatgcggta gtggtccgcc acgtcgccgg tctcactcag gttaaacccg
     6241 gcaaccagca gattcttcag ggcgtactgg tcgagatcgc ggaacagatt cagctgcgcc
     6301 ggtttccccg gccgcagatc cagcagggtg aagtccgggc agtgcgcccg gaagacgtgc
     6361 ggcgcccacg cgtccccttt ggggtcgaag tacaccacag cgtccccgaa atcgcgtacg
     6421 cagcgaatga gcgcgttggt ggccatcgcg ctcttgccgc tcccgggcag gccgaggatc
     6481 tgaatattgg tcttgcgcca ggtatcacga gggaccagca ccggctggcc ctgctcatcc
     6541 ttacccagaa acaccgcctg ctgtttccgg gcctgctccg tggaaaaatc ggccagcgcg
     6601 ttaaccggta ccggctcccc gaaatgcagg tggcggacgt ctgtcatttc tccggtccgc
     6661 tggctgctgc gccggctctc ctgattcagt ttcgcgttca ccgcctcgtc agcggccgca
     6721 aaatgaccat caagcagcag gaacgccagc agccgcgcca gaaggacgcc ggcgacaagc
     6781 cctccggcac accaggctat cccgaacagg ttagccggct gcagcaggta ataatctgcg
     6841 ttcgcataga ggtccgggcg caggacggtt tctccccagc tctcgataaa cacggcgaca
     6901 accagccccc ccagaagggc cagaccggca gcgccggtca gccagtacgc gtagttccag
     6961 acatgtcgcc gtgccctgac catccgcccg ggtgaatcca gcgaggccag caccgaaaat
     7021 ggcacagtcc ggttattatc gcgcttatag cgatcggcat actcccgccg cgccttatgg
     7081 ctcagcccca caaaacgcat tcggccggcc cagagttcag ccagaccgaa aaacaggccg
     7141 ataaccagaa aaaaaccggc cagactgtaa tactcccggt acagccagct gacccacggg
     7201 taaatctgcc gggccagctc cgggatatgc cactgccagg caagcgaatc cgtattaaac
     7261 atgcaatctc tctccttcag acgacgggtt taccggcgtg atgcgcagcc cccgctgcac
     7321 aagccgcgga catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca
     7381 gctttcaccg cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg
     7441 aacaacgagc acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc
     7501 ggtgttccgg cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg
     7561 agcagctttg ctgcttcgtc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc
     7621 gtgagcctga cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc ggacagagcc
     7681 agaaagaggg gaaaagaccg tgcgtcggtg gcgtccagcc agcgtaagca aggtcttttc
     7741 ccctgaagtc agccacgccc cagcggggat gacttgcatt tctccgcaag gagaaaaaag
     7801 caacagagat aggttaacgt aaaatgttca gcgaaaaacc gacatggttc acctatcctg
     7861 ataattgatc gtcagggaat agaaagactt aatcagggga caatatccca catcagcgcg
     7921 tccggtgacc gtcttgcagg tgccggggaa attgcgcctc ctcgctcact cggtcgctac
     7981 gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc
     8041 gtggataagc aggggactaa catgtgaggc aaaacggcag ggccgcgccg gtggcgtttt
     8101 tccataggct ccgccctcct gccagagttc acataaacag acgctttttc ggtgcatctg
     8161 tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa
     8221 agatccccac cgtttccggt aggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg
     8281 tttaccggat acctgtccgc cgttctccct tgcgggaagt gtggcgcttt ctcatagctc
     8341 acacactggt atctcggctc ggtgtaggtc gttcgctcca agctgggctg taagcaagaa
     8401 ctccccgttc agcccgactg ctgcgcctta tccggtaact gttcacttga gtccaacccg
     8461 ggaaagcacg caaaacgcca ctggcagcag ccattggtaa ctgggagttc gcagaggatt
     8521 tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca aagtccggct acactggaag
     8581 gacagatttg gttgctgtgc tctgcgaaag ccagttacca cggttaagca gttccccaac
     8641 tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga
     8701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact gaaccgcgat
     8761 ccccgtcagt tcagaggagg acgatggtgc caccgttccg ccctgaacat cccggatata
     8821 atttgcctga catgcggcca gaagaaagcc agcaacaatc aatataaaaa gaaaaatagt
     8881 ctttaccttt ttcatatact ttccctgtat ttaaagactc cggcattcat ccctgaacac
     8941 cggaagtcag tatttcaaat catccttgat aattttttgt taccagttac cgtcccgata
     9001 atcaaaagct atataacaat tatccacatc aggttttatt tgatttttaa aaaatggctg
     9061 tagtaaagga acccattctt tcttcaaatc aaactcacca ttattaatat tatcctttaa
     9121 aggcattccc aagctttcaa tgacagaacc atcgtcacca aagtctttag aatactcttc
     9181 ccctttaaac tcttcagttt tcttatcaaa ccatgtcaaa cgtaatttaa gccccatatt
     9241 tacctctcaa agatattttt taatgttccg ttttggatct ggccctttaa cctgcttacc
     9301 cgttttggga tcaaatgcgc ctaggtgttc gccatcactg gcacgatacc cttcaagctc
     9361 accgtgctgg gaatcccatt cataaatttt acgtttttta tcgccatacc atcgggcccg
     9421 cttaccacca ccaccttgtt taggtgtttt aggatcacct cgctttaact ctccaagtcc
     9481 ctttatgtct tctgttttag gtactggatg ataatcatgg ccgtagtctt tagtcccctt
     9541 gcgaggcttg ttcttttcct catttaactt attttctgca tccttcgctt ttttctcctt
     9601 ttgtttccgg ctctccatcg ccccggtaag tgcagcctta tctctggata aggattcatt
     9661 cattgcgttt aaagatgcct gagccgcttt taatttctca ttctgattct gaatatcctt
     9721 atcaagatag cgaacctgat cctgccaccc ggagccatat tcatgaggcg ggttataagt
     9781 cacaaaatca tctttatttg cctctgcctc ttttaccttt ttatcggctt cctgaacagc
     9841 agcccttgca ccggggagcg ccttctctgt cgatgcaatg cggctgttta aggtcgcaat
     9901 atttttatct tcggcatcca gctcagcttt cgctttatca tagtctcttt tcagcccctc
     9961 ttccggatga gcggcgtccc atgcctgctg gcgacgcttt tcttcctcct gacgctgttt
    10021 tacctgtgcc ggggtaagaa catctgtcac cgacacataa
//
