LOCUS       NZ_CP050346            10060 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ZRKP03 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP050346
VERSION     NZ_CP050346.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14411775
            Assembly: GCF_015238895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     In Vivo Selection of Meropenem Resistance among Ceftazidime-
            Avibactam-Resistant, Meropenem- Susceptible Klebsiella pneumoniae
            Isolate with KPC-33 Carbapenemase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Bioinformatics center, GensKey Gene
            Technology Co. Ltd., Life Science Park Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-JAN-2020
            Assembly Method                   :: Unicycler v. version 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 117x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 11:30:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,866
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 47 of 144
            Pseudo Genes (incomplete)         :: 99 of 144
            Pseudo Genes (internal stop)      :: 18 of 144
            Pseudo Genes (multiple problems)  :: 17 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050346.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZRKP03"
                     /isolation_source="bronchoalveolar lavage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China"
                     /lat_lon="39.98 N 116.43 E"
                     /collection_date="2019-11-18"
                     /collected_by="Laboratory of Clinical Microbiology and
                     Infectious Diseases, Department of Pulmonary and Critical
                     Care Medicine, China-Japan Friendship Hospital, Beijing, P.
                     R. China"
     gene            749..2713
                     /locus_tag="HB788_RS29010"
                     /old_locus_tag="HB788_29805"
     CDS             749..2713
                     /locus_tag="HB788_RS29010"
                     /old_locus_tag="HB788_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2713..3444
                     /gene="mobC"
                     /locus_tag="HB788_RS29015"
                     /old_locus_tag="HB788_29810"
     CDS             2713..3444
                     /gene="mobC"
                     /locus_tag="HB788_RS29015"
                     /old_locus_tag="HB788_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3451..3981
                     /locus_tag="HB788_RS29020"
                     /old_locus_tag="HB788_29815"
     CDS             3451..3981
                     /locus_tag="HB788_RS29020"
                     /old_locus_tag="HB788_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4008..4187)
                     /locus_tag="HB788_RS29025"
                     /old_locus_tag="HB788_29820"
     CDS             complement(4008..4187)
                     /locus_tag="HB788_RS29025"
                     /old_locus_tag="HB788_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4213..4641)
                     /locus_tag="HB788_RS29030"
                     /old_locus_tag="HB788_29825"
     CDS             complement(4213..4641)
                     /locus_tag="HB788_RS29030"
                     /old_locus_tag="HB788_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4805..5119)
                     /locus_tag="HB788_RS29035"
                     /old_locus_tag="HB788_29830"
     CDS             complement(4805..5119)
                     /locus_tag="HB788_RS29035"
                     /old_locus_tag="HB788_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5107..5385)
                     /locus_tag="HB788_RS29040"
                     /old_locus_tag="HB788_29835"
     CDS             complement(5107..5385)
                     /locus_tag="HB788_RS29040"
                     /old_locus_tag="HB788_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5560..5925)
                     /locus_tag="HB788_RS29425"
     CDS             complement(5560..5925)
                     /locus_tag="HB788_RS29425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5922..6293)
                     /gene="tolA"
                     /locus_tag="HB788_RS29050"
                     /old_locus_tag="HB788_29845"
     CDS             complement(5922..6293)
                     /gene="tolA"
                     /locus_tag="HB788_RS29050"
                     /old_locus_tag="HB788_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6567..6812)
                     /locus_tag="HB788_RS29055"
                     /old_locus_tag="HB788_29850"
     CDS             complement(6567..6812)
                     /locus_tag="HB788_RS29055"
                     /old_locus_tag="HB788_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7139..8824
                     /locus_tag="HB788_RS29060"
                     /old_locus_tag="HB788_29855"
     CDS             7139..8824
                     /locus_tag="HB788_RS29060"
                     /old_locus_tag="HB788_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8834..9091
                     /locus_tag="HB788_RS29065"
                     /old_locus_tag="HB788_29860"
     CDS             8834..9091
                     /locus_tag="HB788_RS29065"
                     /old_locus_tag="HB788_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9176..9325
                     /locus_tag="HB788_RS29070"
                     /old_locus_tag="HB788_29865"
     CDS             9176..9325
                     /locus_tag="HB788_RS29070"
                     /old_locus_tag="HB788_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2139..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9429..10007)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.599"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2714..3441
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cagcgcccgc cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat
       61 ttccccggca cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt
      121 aagtctttct attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc
      181 tgaacatttt acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc
      241 atccccgctg gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc
      301 caccgacgca cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc
      361 agaaattcac ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct
      421 ggacgcacct gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct
      481 atggcgaact ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt
      541 cctctcacac gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg
      601 cggaaagagg ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg
      661 ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg ggctgcgcat cacgccggta
      721 aacccgtcgt ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg
      781 gcatatcccg gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta
      841 ttacagtctg gccggttttt ttctggttat cggcctgttt ttcggtctgg ctgaactctg
      901 ggccggccga atgcgttttg tggggctgag ccataaggcg cggcgggagt atgccgatcg
      961 ctataagcgc gataataacc ggactgtgcc attttcggtg ctggcctcgc tggattcacc
     1021 cgggcggatg gtcagggcac ggcgacatgt ctggaactac gcgtactggc tgaccggcgc
     1081 tgccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     1141 aaccgtcctg cgcccggacc tctatgcgaa cgcagattat tacctgctgc agccggctaa
     1201 cctgttcggg atagcctggt gtgccggagg gcttgtcgcc ggcgtccttc tggcgcggct
     1261 gctggcgttc ctgctgcttg atggtcattt tgcggccgct gacgaggcgg tgaacgcgaa
     1321 actgaatcag gagagccggc gcagcagcca gcggaccgga gaaatgacag acgtccgcca
     1381 cctgcatttc ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc
     1441 ccggaaacag caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc
     1501 tcgtgatacc tggcgcaaga ccaatattca gatcctcggc ctgcccggga gcggcaagag
     1561 cgcgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg ctgtggtgta
     1621 cttcgacccc aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt
     1681 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     1741 ccagtacgcc ctgaagaatc tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt
     1801 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagttccc
     1861 gcagggggca aacattcagc aggtgctggc ggcggcgtac gcgttgccgg aggccctgaa
     1921 aaaggacgtg aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtcctgca
     1981 gacggacagc ggtatcgatg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat
     2041 cggctcgatg gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc
     2101 gcaaatcatc attgcgcggg atgagttccg gaggtggccg cacgcgagca tcatgctcga
     2161 tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg
     2221 ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac ttcggtcagt gcgggcagga
     2281 tctgcccgct gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta
     2341 tcgcgcggcc agccaggagt ccgcgcagtg ggcggcgggc cagaccgggg agatccgggt
     2401 ggacgtggag cgccgccggg ccagccggga ggcggggaac gtggagcaca tcagcggcga
     2461 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacacctgcc
     2521 tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct
     2581 gcgggttgag cgccgggaaa tttcgctgaa aaccttcccg gtgctggcga aaacggaccc
     2641 gctggcggag tacctgcctc cggccgacgg cggcggacca aaagcggatg atgattttgc
     2701 ggggctttac tgatgacact ggagcagtac aacgtcagcc acgcgaagcg gcaggcgcgg
     2761 aacgcggaga aaacacggct gaccctgcgc tggctgcggg aagagctgtg ttccacggcg
     2821 gagctggttg cgcgacggct ggggattgcg gcggtacagc cggtttaccg cttcctggac
     2881 agtctggtgg cgaaagggct gctggtccgg gcgaaatacc cggttgacgg ccgtcaggtc
     2941 agcgtgtggg ggctgacgcc gcacggggtg gcgttctcgt tcgacgagga cgagccgctg
     3001 acggacgtta ttccgtttca gccgtcaagg gtgtcggcgg cgcagctgcc gcaccggctg
     3061 gccgtgcagt ccctgcgcct ctccatggag ggccgggggg ccaccggctg gcggtacctg
     3121 caccgtatgg ccctgaaagg gatgaaagtg ccggacgcgc tggcggagct ggacggcaga
     3181 acggtggcat ttgaggtaga gcgcaccgtc aagagccgca ggcggtatca ggaagtggtg
     3241 tccgggtatc tgttcaaccg ccgggcgaac ggcatcgatg aaatctggta catctgccct
     3301 gaccggccga cgcttgtccg ggtacagcgg gcgattctgg cggtggatga aatcgtgaat
     3361 ccacagaccg gcgaggcccg caaaacggtg gagctggacc gggagaggct gttcgcgtgc
     3421 tttaaattta tgacaacgga gtgagggatt atggcgctgt acatggtatt tattgaggac
     3481 gtggaagaca aagacggcga gcgctgcatt atgccaacca gcgagcatct ggaagacatg
     3541 gtggtgctcg cctggcagac cggggcgtat gttttctgtt ttgtcgaccg ggcccagcat
     3601 ctggcgatga tgaaatccat cgggatgtat gacgagcggc tcaacagcga cgaaaaagcc
     3661 acgtactttt tcaccggtga aaatccgcaa tgggaacggg acaaacccac aggaatgaag
     3721 ctgtgtgatt acctctatcg aaaggtggag cggttcgcca tcgagaacgg tgtgcaggtg
     3781 gttgatgccg ggaagtgctc accggagcag attaacgcga tgattgaggt ggtcagcgag
     3841 cggcacgggg aaagcgcggt gaacgagaag ctgaaagcgg agctgatgcg ggaatataac
     3901 agccgctatt ttaacgtgcc ggtgcctgaa ctgccggagg acaaacccgc ccgccggtgg
     3961 tggccgttcg gcaaaaaata acgccagcac cggcatggcc ggtgctgtta ttcttccccg
     4021 agcttatccc gcatcacctg ccagagtgtt tccgcatcct cgtgaagccg ttcgcacatg
     4081 tcggccgggg tatccatgtc cagctcgttc agcttttcca gcaacagcag cgactgatcc
     4141 ttaatgaatt tagccatatt gagggcagtt ttttcctgct tcgtcatgtt ctccattccc
     4201 ttcggtaagt tctcagctaa accagccgga ccagaagcgt tttttcttct gtgtcagttc
     4261 tgttgtcaat ctgtccactt cggcctggag tttccggcgc tcatcatcgt gctggcggcg
     4321 ttcttcttcc cgggatgttt tatcttccag catcagggtg acagcctgcc ggagatcggc
     4381 cagttccttt tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg tattttcacg
     4441 gtgtgaaatg ccgggaattt gtttctcagg gatattcaca tttccgtata cacggatcag
     4501 ttctgacaca tccacgacag ggttgttttt cccgtcacgt gacacggtaa cctttccctg
     4561 ttttacatga ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg cctgtgaaag
     4621 gttgagcaat gttttcgcca ctgtataccc ctgattttat cgggtgtgaa aggtgtgtaa
     4681 tttctcacgg taaatgtatc gctgaatttt acccgttttt ccgttcagga tgtggttttc
     4741 tgacgcgctg cgcttgtcaa accggcgacg gccacgcaat gaattgcgcc cccgtcatcg
     4801 gttattagag gctgtctgcc cccggatacc cggattcttt ctgatgcctg acggtgacga
     4861 tcacaatctg gtccatttcc cggtcgtggc ggtagagcat cacatagcca ctgtcgccaa
     4921 acccgatcac cagttcctta tattccagcg gcagaaacgg caccgggcgg ccaatgtctg
     4981 gcaatgtttt cagttgctgg atagcccgga cgatcacctc acctcctttt ctggctgcca
     5041 gccgattttt ggttttgaga aagtcctgaa ggcgttgtaa atcgcgctgt gccagcgcgg
     5101 aaatcgttac ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc aggtacttat
     5161 ccaggcttcc gcttcttccg cagtaaggtg cagacccgtt tcctgatagt gctgccatgc
     5221 ggcctgtccg tcccgcaggt actggctgcg cttctcttcc cggtcgatgt attcggtgat
     5281 tgcctccagc attagcgcgt gcgctgagcg gtgccggtca tcggccagcg ttttcagccg
     5341 gtccttaagt tcttcatcga gcctgatgga tgttgctgat gacataaaag cccctttgta
     5401 gtcatatgta ttacacatga ctactttaat ccgtggcggc gctatttgcc agaaatacgg
     5461 cttttgctgc ccatttttca gcttcgcttc cactaatcag ggctgctaca ggctcactca
     5521 tcatccagga ctattgcagc ctgcgggtta aaggacctgt tactggcggc aaccatcatc
     5581 attgagcttt tgggtggtga ctggtcgcgg atcctgtaag ccgtcatttt tcagactgaa
     5641 ttcaaaattt ataacctgtc gcattgccgg gtgccctgcg ggatatagcc agtgcctcat
     5701 tgccgtcagc gtttcccgaa aaaaaaggtg acagccggta agcgacacgt tgtccactcg
     5761 tccatcgtcc agaatatcaa acgtggcggt cacctgacct tcgatgcgtt ttgctgcggc
     5821 ctgtaccggg taggttggct gcagtgtggt tacgggtttt agtggtatcg catgtaccgg
     5881 agtgaggaac atggccgcca gcactgtcag agtaaaagct ttcatggtct gaaatccaga
     5941 ggggcgtttt tgaatatttg ccaggtattt tcatccggtg ccggcggaat tttggcgtgg
     6001 gtaacggctg atattgctgc tttacagagt ttcggatctc ctccttcagc aatggcgctg
     6061 ttcagagtgc catcacgttg tatggatatc cgcacggtgc acatttttcc cgcataggcc
     6121 tctggttcaa acatctgcat ttcaacagcg gtcttgatgg ccgcagcgta gctactgatt
     6181 ttagatgact tatccagagt ataaattttc ggcggctggg tggtggaatg cttacaggcc
     6241 gtcaggagta aggggctgca caatgccagc aggataaagg gtattaaacg catcgggttg
     6301 atgacctgtc agtaaaaaat gttacatgta tatttgtcac tctttacggc cacgttcatt
     6361 tacataaaca cggcttatcg tatcgttttt acaccaaacg gcccgccatg cagttgcgtg
     6421 cataggcatg cagcgggtta caatttaccg ggcgcgtttc cggcggtttt ccgggtggtt
     6481 tgttgcctgt tttatcccgt agccgccgga aacgccctca gaccgtctga gcggtgcggg
     6541 caatgcgacg ttatggtaaa taccccttac cggcggcgct gtggctgcca ggtgaaggtg
     6601 gtttccgtag cctcgtattt ctcccgacgc atttcctgcg cccgcctggc ttcacgttcg
     6661 tcctgaatgg cacgaagcac cctttcccgc acctgttgtt ttaccgcctt tggcagcgtt
     6721 ttgttccctt tcaggcccat ggccagtgcc tgagcgacaa aggcgtccgc caggcgcttt
     6781 tcttcagcgg gagtcagttt caggtgcgtc atagtggcct ccgggcgtct caggtttccc
     6841 gaacagttta accgcaggtc ctctcctcat gccagttagc tttcagatgt gtctatacca
     6901 tctgaaatgt aagtgtgatg aatgtcttaa ataataaaat ttgttgccat gagaaatttt
     6961 cgggaaaaga cgcgatctgc ttacaggtgg ctccgggcgc ttttttgtgc ctgaagatga
     7021 gaactaatct catatattga cacctgaaaa cgggaggagt aaggtaataa ttatactgtg
     7081 tatatgtaca gtatgttggc tttaatttat tgttttaaat gtcaaagagg aaaacggtat
     7141 gagtggtgga gacggtcgag gtccgggtaa ttcaggtctg ggacataatg gtggtcaggc
     7201 cagtgggaat gtgaacggta cgtctggtaa aggtggccct tcatcaggtg gtggtacgga
     7261 tccaaacagc gggccgggct ggggtacgac gcatacgccg aatggagata ttcataacta
     7321 taatccgggg gagtttggta acggcgggag taaacccggt ggtaatggtg gtaacagcgg
     7381 caatcatagc ggtagctctg gtggcggaca gtcttcggcc actgcgatgg ccttcggtct
     7441 gcctgccctg gctactccgg gggctgaagg actggcttta tcagtttccg gcgatgcgtt
     7501 gtcggccgcc gttgctgatg tgctggctgc cctgaagggg ccgtttaagt ttggtctgtg
     7561 ggggattgcg atctacggtg tgctgccttc tgagattgca aaagatgatc cgaaaatgat
     7621 gtcaaaaatt atgacgtcat taccggccga tgcggtgacg gagactccgg caagtacttt
     7681 accactggac caggcgacgg ttcgtgtcag acaacgggtt gtggatgtgg tgaaggatga
     7741 gcggcagcat attgcggttg tcgcaggtcg gccaatgagt gtccctgtgg tggatgcgaa
     7801 accgacaaaa cgtccggggg tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt
     7861 gagcgtaccg aaaggtgttc cgacagcgaa agccccgcca aaaggcattg ttgctgaaaa
     7921 aggtgattca cgtccggctg gttttacagc cggtggtaac tcccgtgagg ccgttattcg
     7981 tttcccgaaa gagaccggac agaagccggt ttatgtgtcg gtgacagatg ttcttacccc
     8041 ggcacaggta aaacagcgtc aggaggaaga aaagcgtcgc cagcaggcat gggacgccgc
     8101 tcatccggaa gaggggctga aaagagacta tgataaagcg aaagctgagc tggatgccga
     8161 agataaaaat attgcgacct taaacagccg cattgcatcg acagagaagg cgctccccgg
     8221 tgcaagggct gctgttcagg aagccgataa aaaggtaaaa gaggcagagg caaataaaga
     8281 tgattttgtg acttataacc cgcctcatga atatggctcc gggtggcagg atcaggttcg
     8341 ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagcggctc aggcatcttt
     8401 aaacgcaatg aatgaatcct tatccagaga taaggctgca cttaccgggg cgatggagag
     8461 ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aagttaaatg aggaaaagaa
     8521 caagcctcgc aaggggacta aagactacgg ccatgattat catccagtac ctaaaacaga
     8581 agacataaag ggacttggag agttaaagcg aggtgatcct aaaacaccta aacaaggtgg
     8641 tggtggtaag cgggcccgat ggtatggcga taaaaaacgt aaaatttatg aatgggattc
     8701 ccagcacggt gagcttgaag ggtatcgtgc cagtgatggc gaacacctag gcgcatttga
     8761 tcccaaaacg ggtaagcagg ttaaagggcc agatccaaaa cggaacatta aaaaatatct
     8821 ttgagaggta aatatggggc ttaaattacg tttgacatgg tttgataaga aaactgaaga
     8881 gtttaaaggg gaagagtatt ctaaagactt tggtgacgat ggttctgtca ttgaaagctt
     8941 gggaatgcct ttaaaggata atattaataa tggtgagttt gatttgaaga aagaatgggt
     9001 tcctttacta cagccatttt ttaaaaatca aataaaacct gatgtggata attgttatat
     9061 agcttttgat tatcgggacg gtaactggta acaaaaaatt atcaaggatg atttgaaata
     9121 ctgacttccg gtgttcaggg atgaatgccg gagtctttaa atacagggaa agtatatgaa
     9181 aaaggtaaag actatttttc tttttatatt gattgttgct ggctttcttc tggccgcatg
     9241 tcaggcaaat tatatccggg atgttcaggg cggaacggtg gcaccatcgt cctcctctga
     9301 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt
     9361 ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa
     9421 ggttaagtca gttggggaac tgcttaaccg ggtaactggc tttcgcagag cacagcaacc
     9481 aaatctgtcc ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt
     9541 agctaaacaa atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgtttttg
     9601 cgtgtctttc cgggttggac tcaagatgac agttaccgga taaggcgcag cagtcgggct
     9661 gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc gagccgagat
     9721 accagtgtgt gagctatgag aaagcgccac acttcccgca agggagaacg gcggacaggt
     9781 atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgacct accggaaacg
     9841 gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg gttgagcctc
     9901 acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc aggagggcgg
     9961 agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg ttagtcccct
    10021 gcttatccac ggaatctgtg ggtaactttg tatgtgtccg
//
