LOCUS       NZ_CP050340            10049 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain ZRKP04 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP050340
VERSION     NZ_CP050340.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14411776
            Assembly: GCF_015238655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     In Vivo Selection of Meropenem Resistance among Ceftazidime-
            Avibactam-Resistant, Meropenem- Susceptible Klebsiella pneumoniae
            Isolate with KPC-33 Carbapenemase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   Wang,C., Liu,Z., Sun,A., Li,L., Sun,L., Zhao,J., Li,B., Lu,B.,
            Liu,Y. and Cao,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2020) Bioinformatics center, GensKey Gene
            Technology Co. Ltd., Life Science Park Road, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-JAN-2020
            Assembly Method                   :: Unicycler v. version 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 111x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 13:57:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,902
            CDSs (total)                      :: 5,780
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 49 of 154
            Pseudo Genes (incomplete)         :: 108 of 154
            Pseudo Genes (internal stop)      :: 19 of 154
            Pseudo Genes (multiple problems)  :: 18 of 154
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050340.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ZRKP04"
                     /isolation_source="bronchoalveolar lavage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China"
                     /lat_lon="39.98 N 116.43 E"
                     /collection_date="2019-11-23"
                     /collected_by="Laboratory of Clinical Microbiology and
                     Infectious Diseases, Department of Pulmonary and Critical
                     Care Medicine, China-Japan Friendship Hospital, Beijing, P.
                     R. China"
     gene            complement(join(9686..10049,1..368))
                     /gene="mobC"
                     /locus_tag="HB789_RS29180"
                     /old_locus_tag="HB789_29965"
     CDS             complement(join(9686..10049,1..368))
                     /gene="mobC"
                     /locus_tag="HB789_RS29180"
                     /old_locus_tag="HB789_29965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(368..2332)
                     /locus_tag="HB789_RS29185"
                     /old_locus_tag="HB789_29970"
     CDS             complement(368..2332)
                     /locus_tag="HB789_RS29185"
                     /old_locus_tag="HB789_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3464..3622
                     /locus_tag="HB789_RS29810"
     CDS             3464..3622
                     /locus_tag="HB789_RS29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            complement(3805..3954)
                     /locus_tag="HB789_RS29190"
                     /old_locus_tag="HB789_29975"
     CDS             complement(3805..3954)
                     /locus_tag="HB789_RS29190"
                     /old_locus_tag="HB789_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4039..4296)
                     /locus_tag="HB789_RS29195"
                     /old_locus_tag="HB789_29980"
     CDS             complement(4039..4296)
                     /locus_tag="HB789_RS29195"
                     /old_locus_tag="HB789_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(4306..5991)
                     /locus_tag="HB789_RS29200"
                     /old_locus_tag="HB789_29985"
     CDS             complement(4306..5991)
                     /locus_tag="HB789_RS29200"
                     /old_locus_tag="HB789_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6318..6563
                     /locus_tag="HB789_RS29205"
                     /old_locus_tag="HB789_29990"
     CDS             6318..6563
                     /locus_tag="HB789_RS29205"
                     /old_locus_tag="HB789_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6837..7208
                     /gene="tolA"
                     /locus_tag="HB789_RS29210"
                     /old_locus_tag="HB789_29995"
     CDS             6837..7208
                     /gene="tolA"
                     /locus_tag="HB789_RS29210"
                     /old_locus_tag="HB789_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            7205..7570
                     /locus_tag="HB789_RS29605"
     CDS             7205..7570
                     /locus_tag="HB789_RS29605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7745..8023
                     /locus_tag="HB789_RS29220"
                     /old_locus_tag="HB789_30005"
     CDS             7745..8023
                     /locus_tag="HB789_RS29220"
                     /old_locus_tag="HB789_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            8011..8325
                     /locus_tag="HB789_RS29225"
                     /old_locus_tag="HB789_30010"
     CDS             8011..8325
                     /locus_tag="HB789_RS29225"
                     /old_locus_tag="HB789_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8489..8917
                     /locus_tag="HB789_RS29230"
                     /old_locus_tag="HB789_30015"
     CDS             8489..8917
                     /locus_tag="HB789_RS29230"
                     /old_locus_tag="HB789_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8943..9122
                     /locus_tag="HB789_RS29235"
                     /old_locus_tag="HB789_30020"
     CDS             8943..9122
                     /locus_tag="HB789_RS29235"
                     /old_locus_tag="HB789_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9149..9679)
                     /locus_tag="HB789_RS29240"
                     /old_locus_tag="HB789_30025"
     CDS             complement(9149..9679)
                     /locus_tag="HB789_RS29240"
                     /old_locus_tag="HB789_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10049
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3135..3702
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..368)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.902"
                     /coverage="99.0"
                     /evalue="3.0899999999999997e-60"
     Typing          complement(9690..10045)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.597"
                     /coverage="99.0"
                     /evalue="3.1199999999999996e-67"
ORIGIN
        1 aggcgcaggg actgcacggc cagccggtgc ggcagctgcg ccgccgacac ccttgacggc
       61 tgaaacggaa taacgtccgt cagcggctcg tcctcgtcga acgagaacgc caccccgtgc
      121 ggcgtcagcc cccacacgct gacctgacgg ccgtcaaccg ggtatttcgc ccggaccagc
      181 agccctttcg ccaccagact gtccaggaag cggtaaaccg gctgtaccgc cgcaatcccc
      241 agccgtcgcg caaccagctc cgccgtggaa cacagctctt cccgcagcca gcgcagggtc
      301 agccgtgttt tctccgcgtt ccgcgcctgc cgcttcgcgt ggctgacgtt gtactgctcc
      361 agtgtcatca gtaaagcccc gcaaaatcat catccgcttt tggtccgccg ccgtcggccg
      421 gaggcaggta ctccgccagc gggtccgttt tcgccagcac cgggaaggtt ttcagcgaaa
      481 tttcccggcg ctcaacccgc agcgggctgc tgaacgccag ccgcgccacg cccagcccgg
      541 tcatcaccgc gaacccgtca ggcaggtgtt gcagggtgtt cacgtcaaac agcggccgcg
      601 cctccttctg gatgaagctg tcgccgctga tgtgctccac gttccccgcc tcccggctgg
      661 cccggcggcg ctccacgtcc acccggatct ccccggtctg gcccgccgcc cactgcgcgg
      721 actcctggct ggccgcgcga tagaaccagc ggatcggcgt gttatccagt accgtggttt
      781 tcacaaaatc agcgggcaga tcctgcccgc actgaccgaa gtcgcccagc gactggtgcg
      841 cgagcagcag gttgcagtcc cggctgcgca gcgtccccag cgcgttcagc acgtacttcg
      901 acagcagata tttaatctca tcgagcatga tgctcgcgtg cggccacctc cggaactcat
      961 cccgcgcaat gatgatttgc gcgcagcggg caaacagcat cttctgcacc cggatcaccg
     1021 cctcatcgtc catcgagccg atgacgtaca ggcagccgcc gccgttgatt atccccgcca
     1081 catcgatacc gctgtccgtc tgcaggacgc tcaggtcggc cacgttctcc agcttggtga
     1141 tcagcccctt cacgtccttt ttcagggcct ccggcaacgc gtacgccgcc gccagcacct
     1201 gctgaatgtt tgccccctgc gggaactgct ccgcaatcag cttcgccgct ttctgctcgc
     1261 tgatgcggta gtggtccgcc acgtcgccgg tctcactcag gttaaacccg gcaaccagca
     1321 gattcttcag ggcgtactgg tcgagatcgc ggaacagatt cagctgcgcc ggtttccccg
     1381 gccgcagatc cagcagggtg aagtccgggc agtgcgcccg gaagacgtgc ggcgcccacg
     1441 cgtccccttt ggggtcgaag tacaccacag cgtccccgaa atcgcgtacg cagcgaatga
     1501 gcgcgttggt ggccatcgcg ctcttgccgc tcccgggcag gccgaggatc tgaatattgg
     1561 tcttgcgcca ggtatcacga gggaccagca ccggctggcc ctgctcatcc ttacccagaa
     1621 acaccgcctg ctgtttccgg gcctgctccg tggaaaaatc ggccagcgcg ttaaccggta
     1681 ccggctcccc gaaatgcagg tggcggacgt ctgtcatttc tccggtccgc tggctgctgc
     1741 gccggctctc ctgattcagt ttcgcgttca ccgcctcgtc agcggccgca aaatgaccat
     1801 caagcagcag gaacgccagc agccgcgcca gaaggacgcc ggcgacaagc cctccggcac
     1861 accaggctat cccgaacagg ttagccggct gcagcaggta ataatctgcg ttcgcataga
     1921 ggtccgggcg caggacggtt tctccccagc tctcgataaa cacggcgaca accagccccc
     1981 ccagaagggc cagaccggca gcgccggtca gccagtacgc gtagttccag acatgtcgcc
     2041 gtgccctgac catccgcccg ggtgaatcca gcgaggccag caccgaaaat ggcacagtcc
     2101 ggttattatc gcgcttatag cgatcggcat actcccgccg cgccttatgg ctcagcccca
     2161 caaaacgcat tcggccggcc cagagttcag ccagaccgaa aaacaggccg ataaccagaa
     2221 aaaaaccggc cagactgtaa tactcccggt acagccagct gacccacggg taaatctgcc
     2281 gggccagctc cgggatatgc cactgccagg caagcgaatc cgtattaaac atgcaatctc
     2341 tctccttcag acgacgggtt taccggcgtg atgcgcagcc cccgctgcac aagccgcgga
     2401 catgccccaa ggcatggcgg cggcggtcac gtccgtggtg attgcgttca gctttcaccg
     2461 cggaggccag cctctttccg ccccatgcgg aaaaggcgca gccccggagg aacaacgagc
     2521 acgccccagc gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg
     2581 cccccgtcag agttcgccat agcgagcggc tgaccacccg caaaatcagg agcagctttg
     2641 ctgcttcgtc aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga
     2701 cgggggccgg gtgaatttct ggcgtggccg gcgtccagcc ggacagagcc agaaagaggg
     2761 gaaaagaccg tgcgtcggtg gcgtccagcc agcgtaagca aggtcttttc ccctgaagtc
     2821 agccacgccc cagcggggat gacttgcatt tctccgcaag gagaaaaaag caacagagat
     2881 aggttaacgt aaaatgttca gcgaaaaacc gacatggttc acctatcctg ataattgatc
     2941 gtcagggaat agaaagactt aatcagggga caatatccca catcagcgcg tccggtgacc
     3001 gtcttgcagg tgccggggaa attgcgcctc ctcgctcact cggtcgctac gctccgggcg
     3061 tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc gtggataagc
     3121 aggggactaa catgtgaggc aaaacggcag ggccgcgccg gtggcgtttt tccataggct
     3181 ccgccctcct gccagagttc acataaacag acgctttttc ggtgcatctg tgggagccgt
     3241 gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac
     3301 cgtttccggt aggtcgctcc ctcgtgcgct ctcctgttcc gaccctgccg tttaccggat
     3361 acctgttccg cctttttccc tttcgggaag cgtggcgctt tctcatagct cacgctgctg
     3421 gtatctcggc tcggtttagg tcgttcgctc caagctgggc tgtatgcacg aaccccccgt
     3481 tcagcccgac tgctgcgccc tttccggtaa ctatcatctt gagtccaacc cggacggaca
     3541 cgcaaaaacg ccactggcag cagccattgg taactggata tatacgagag agatattgag
     3601 aggtcttgaa gtgggtgcct aactgcggct acactgaaag aacagttttg gtatctgcgg
     3661 tctcgtatac ccagttaccc ggttaagcag ttccccaact gacttaacct tcgatcaaac
     3721 cgcctcccca ggcggttttt tcgtttacag tgcaagagat tacgcgcaga aaaaaaggat
     3781 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga
     3841 cgatggtgcc accgttccgc cctgaacatc ccggatataa tttgcctgac atgcggccag
     3901 aagaaagcca gcaacaatca atataaaaag aaaaatagtc tttacctttt tcatatactt
     3961 tccctgtatt taaagactcc ggcattcatc cctgaacacc ggaagtcagt atttcaaatc
     4021 atccttgata attttttgtt accagttacc gtcccgataa tcaaaagcta tataacaatt
     4081 atccacatca ggttttattt gatttttaaa aaatggctgt agtaaaggaa cccattcttt
     4141 cttcaaatca aactcaccat tattaatatt atcctttaaa ggcattccca agctttcaat
     4201 gacagaacca tcgtcaccaa agtctttaga atactcttcc cctttaaact cttcagtttt
     4261 cttatcaaac catgtcaaac gtaatttaag ccccatattt acctctcaaa gatatttttt
     4321 aatgttccgt tttggatctg gccctttaac ctgcttaccc gttttgggat caaatgcgcc
     4381 taggtgttcg ccatcactgg cacgataccc ttcaagctca ccgtgctggg aatcccattc
     4441 ataaatttta cgttttttat cgccatacca tcgggcccgc ttaccaccac caccttgttt
     4501 aggtgtttta ggatcacctc gctttaactc tccaagtccc tttatgtctt ctgttttagg
     4561 tactggatga taatcatggc cgtagtcttt agtccccttg cgaggcttgt tcttttcctc
     4621 atttaactta ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc
     4681 cccggtaagt gcagccttat ctctggataa ggattcattc attgcgttta aagatgcctg
     4741 agccgctttt aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc
     4801 ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc
     4861 ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggagcgc
     4921 cttctctgtc gatgcaatgc ggctgtttaa ggtcgcaata tttttatctt cggcatccag
     4981 ctcagctttc gctttatcat agtctctttt cagcccctct tccggatgag cggcgtccca
     5041 tgcctgctgg cgacgctttt cttcctcctg acgctgtttt acctgtgccg gggtaagaac
     5101 atctgtcacc gacacataaa ccggcttctg tccggtctct ttcgggaaac gaataacggc
     5161 ctcacgggag ttaccaccgg ctgtaaaacc agccggacgt gaatcacctt tttcagcaac
     5221 aatgcctttt ggcggggctt tcgctgtcgg aacacctttc ggtacgctca cctgcagaga
     5281 cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac
     5341 cacagggaca ctcattggcc gacctgcgac aaccgcaata tgctgccgct catccttcac
     5401 cacatccaca acccgttgtc tgacacgaac cgtcgcctgg tccagtggta aagtacttgc
     5461 cggagtctcc gtcaccgcat cggccggtaa tgacgtcata atttttgaca tcattttcgg
     5521 atcatctttt gcaatctcag aaggcagcac accgtagatc gcaatccccc acagaccaaa
     5581 cttaaacggc cccttcaggg cagccagcac atcagcaacg gcggccgaca acgcatcgcc
     5641 ggaaactgat aaagccagtc cttcagcccc cggagtagcc agggcaggca gaccgaaggc
     5701 catcgcagtg gccgaagact gtccgccacc agagctaccg ctatgattgc cgctgttacc
     5761 accattacca ccgggtttac tcccgccgtt accaaactcc cccggattat agttatgaat
     5821 atctccattc ggcgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc
     5881 acctgatgaa gggccacctt taccagacgt accgttcaca ttcccactgg cctgaccacc
     5941 attatgtccc agacctgaat tacccggacc tcgaccgtct ccaccactca taccgttttc
     6001 ctctttgaca tttaaaacaa taaattaaag ccaacatact gtacatatac acagtataat
     6061 tattacctta ctcctcccgt tttcaggtgt caatatatga gattagttct catcttcagg
     6121 cacaaaaaag cgcccggagc cacctgtaag cagatcgcgt cttttcccga aaatttctca
     6181 tggcaacaaa ttttattatt taagacattc atcacactta catttcagat ggtatagaca
     6241 catctgaaag ctaactggca tgaggagagg acctgcggtt aaactgttcg ggaaacctga
     6301 gacgcccgga ggccactatg acgcacctga aactgactcc cgctgaagaa aagcgcctgg
     6361 cggacgcctt tgtcgctcag gcactggcca tgggcctgaa agggaacaaa acgctgccaa
     6421 aggcggtaaa acaacaggtg cgggaaaggg tgcttcgtgc cattcaggac gaacgtgaag
     6481 ccaggcgggc gcaggaaatg cgtcgggaga aatacgaggc tacggaaacc accttcacct
     6541 ggcagccaca gcgccgccgg taaggggtat ttaccataac gtcgcattgc ccgcaccgct
     6601 cagacggtct gagggcgttt ccggcggcta cgggataaaa caggcaacaa accacccgga
     6661 aaaccgccgg aaacgcgccc ggtaaattgt aacccgctgc atgcctatgc acgcaactgc
     6721 atggcgggcc gtttggtgta aaaacgatac gataagccgt gtttatgtaa atgaacgtgg
     6781 ccgtaaagag tgacaaatat acatgtaaca ttttttactg acaggtcatc aacccgatgc
     6841 gtttaatacc ctttatcctg ctggcattgt gcagcccctt actcctgacg gcctgtaagc
     6901 attccaccac ccagccgccg aaaatttata ctctggataa gtcatctaaa atcagtagct
     6961 acgctgcggc catcaagacc gctgttgaaa tgcagatgtt tgaaccagag gcctatgcgg
     7021 gaaaaatgtg caccgtgcgg atatccatac aacgtgatgg cactctgaac agcgccattg
     7081 ctgaaggagg agatccgaaa ctctgtaaag cagcaatatc agccgttacc cacgccaaaa
     7141 ttccgccggc accggatgaa aatacctggc aaatattcaa aaacgcccct ctggatttca
     7201 gaccatgaaa gcttttactc tgacagtgct ggcggccatg ttcctcactc cggtacatgc
     7261 gataccacta aaacccgtaa ccacactgca gccaacctac ccggtacagg ccgcagcaaa
     7321 acgcatcgaa ggtcaggtga ccgccacgtt tgatattctg gacgatggac gagtggacaa
     7381 cgtgtcgctt accggctgtc accttttttt tcgggaaacg ctgacggcaa tgaggcactg
     7441 gctatatccc gcagggcacc cggcaatgcg acaggttata aattttgaat tcagtctgaa
     7501 aaatgacggc ttacaggatc cgcgaccagt caccacccaa aagctcaatg atgatggttg
     7561 ccgccagtaa caggtccttt aacccgcagg ctgcaatagt cctggatgat gagtgagcct
     7621 gtagcagccc tgattagtgg aagcgaagct gaaaaatggg cagcaaaagc cgtatttctg
     7681 gcaaatagcg ccgccacgga ttaaagtagt catgtgtaat acatatgact acaaaggggc
     7741 ttttatgtca tcagcaacat ccatcaggct cgatgaagaa cttaaggacc ggctgaaaac
     7801 gctggccgat gaccggcacc gctcagcgca cgcgctaatg ctggaggcaa tcaccgaata
     7861 catcgaccgg gaagagaagc gcagccagta cctgcgggac ggacaggccg catggcagca
     7921 ctatcaggaa acgggtctgc accttactgc ggaagaagcg gaagcctgga taagtacctg
     7981 gggaactgag aacgagcagg atgctccgcc atgccacagg taacgatttc cgcgctggca
     8041 cagcgcgatt tacaacgcct tcaggacttt ctcaaaacca aaaatcggct ggcagccaga
     8101 aaaggaggtg aggtgatcgt ccgggctatc cagcaactga aaacattgcc agacattggc
     8161 cgcccggtgc cgtttctgcc gctggaatat aaggaactgg tgatcgggtt tggcgacagt
     8221 ggctatgtga tgctctaccg ccacgaccgg gaaatggacc agattgtgat cgtcaccgtc
     8281 aggcatcaga aagaatccgg gtatccgggg gcagacagcc tctaataacc gatgacgggg
     8341 gcgcaattca ttgcgtggcc gtcgccggtt tgacaagcgc agcgcgtcag aaaaccacat
     8401 cctgaacgga aaaacgggta aaattcagcg atacatttac cgtgagaaat tacacacctt
     8461 tcacacccga taaaatcagg ggtatacagt ggcgaaaaca ttgctcaacc tttcacaggc
     8521 agcacaagcc gcgggcatta ccagaagaac gctatacaat catgtaaaac agggaaaggt
     8581 taccgtgtca cgtgacggga aaaacaaccc tgtcgtggat gtgtcagaac tgatccgtgt
     8641 atacggaaat gtgaatatcc ctgagaaaca aattcccggc atttcacacc gtgaaaatac
     8701 acaaaaaaat ttcccacagg aacagcttct ggcaatgcaa aaggaactgg ccgatctccg
     8761 gcaggctgtc accctgatgc tggaagataa aacatcccgg gaagaagaac gccgccagca
     8821 cgatgatgag cgccggaaac tccaggccga agtggacaga ttgacaacag aactgacaca
     8881 gaagaaaaaa cgcttctggt ccggctggtt tagctgagaa cttaccgaag ggaatggaga
     8941 acatgacgaa gcaggaaaaa actgccctca atatggctaa attcattaag gatcagtcgc
     9001 tgctgttgct ggaaaagctg aacgagctgg acatggatac cccggccgac atgtgcgaac
     9061 ggcttcacga ggatgcggaa acactctggc aggtgatgcg ggataagctc ggggaagaat
     9121 aacagcaccg gccatgccgg tgctggcgtt attttttgcc gaacggccac caccggcggg
     9181 cgggtttgtc ctccggcagt tcaggcaccg gcacgttaaa atagcggctg ttatattccc
     9241 gcatcagctc cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca
     9301 cctcaatcat cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac
     9361 cgttctcgat ggcgaaccgc tccacctttc gatagaggta atcacacagc ttcattcctg
     9421 tgggtttgtc ccgttcccat tgcggatttt caccggtgaa aaagtacgtg gctttttcgt
     9481 cgctgttgag ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc
     9541 ggtcgacaaa acagaaaaca tacgccccgg tctgccaggc gagcaccacc atgtcttcca
     9601 gatgctcgct ggttggcata atgcagcgct cgccgtcttt gtcttccacg tcctcaataa
     9661 ataccatgta cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcgaaca
     9721 gcctctcccg gtccagctcc accgttttgc gggcctcgcc ggtctgtgga ttcacgattt
     9781 catccaccgc cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca gggcagatgt
     9841 accagatttc atcgatgccg ttcgcccggc ggttgaacag atacccggac accacttcct
     9901 gataccgcct gcggctcttg acggtgcgct ctacctcaaa tgccaccgtt ctgccgtcca
     9961 gctccgccag cgcgtccggc actttcatcc ctttcagggc catacggtgc aggtaccgcc
    10021 agccggtggc cccccggccc tccatggag
//
