LOCUS       NZ_CP050072             5406 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella aerogenes strain 035 plasmid p35_E, complete sequence.
ACCESSION   NZ_CP050072
VERSION     NZ_CP050072.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14372988
            Assembly: GCF_011604725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Nuesch-Inderbinen,M., Stephan,R. and Stevens,M.J.A.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Nuesch-Inderbinen,M., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAR-2020
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: ILS_035_Kaerogenes
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:50:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,267
            CDSs (total)                      :: 5,140
            Genes (coding)                    :: 5,004
            CDSs (with protein)               :: 5,004
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 44 of 136
            Pseudo Genes (incomplete)         :: 95 of 136
            Pseudo Genes (internal stop)      :: 19 of 136
            Pseudo Genes (multiple problems)  :: 19 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050072.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="035"
                     /isolation_source="water"
                     /db_xref="taxon:548"
                     /plasmid="p35_E"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            join(4793..5406,1..84)
                     /locus_tag="HA513_RS26260"
                     /old_locus_tag="HA513_26260"
     CDS             join(4793..5042,5042..5406,1..84)
                     /locus_tag="HA513_RS26260"
                     /old_locus_tag="HA513_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_225248593.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGWKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            1042..1233
                     /locus_tag="HA513_RS26265"
                     /old_locus_tag="HA513_26265"
     CDS             1042..1233
                     /locus_tag="HA513_RS26265"
                     /old_locus_tag="HA513_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_050074851.1"
                     /translation="MNKEQKTALNMAKFIQGQSLLLLEKLNALDFDTEADLCEKLHEDT
                     EQLYRVLSERLKESEDGY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1233..1664
                     /locus_tag="HA513_RS26270"
                     /old_locus_tag="HA513_26270"
     CDS             1233..1664
                     /locus_tag="HA513_RS26270"
                     /old_locus_tag="HA513_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135007.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="WP_166479956.1"
                     /translation="MAKVSISEAARLVNVSRPTLYKMLNNGTLTYTSVVKHGKQVKTID
                     TSELIRVFGSLGGVKDDTQNAVSVDGHATAINSDILQDLQHQIALLKTENDGLKGAVNA
                     RDEHISSLRQAMLLLEHKQEQLAPQISSPAHSPWWKFWK"
                     /name="excisionase family DNA-binding protein"
     gene            2922..3164
                     /locus_tag="HA513_RS26275"
                     /old_locus_tag="HA513_26275"
     CDS             2922..3164
                     /locus_tag="HA513_RS26275"
                     /old_locus_tag="HA513_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_137399573.1"
                     /translation="MATMNIRIDDDLKARSGAALDALGVTTSEAVRLVLEYVAQNNRLP
                     VQIALLADEDADIIELARARLANPGKRIRVKLDDL"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            3154..3453
                     /locus_tag="HA513_RS26280"
                     /old_locus_tag="HA513_26280"
     CDS             3154..3453
                     /locus_tag="HA513_RS26280"
                     /old_locus_tag="HA513_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143805.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_137399574.1"
                     /translation="MTYDIEFYEEAFDEWHRLDASVRDQFKKKLAKLVEDPHRPANRLR
                     GLEGCYKIKLRAAGVRLVYKVEDGELVILVVAVGKRDKNAVYDTARERLVTIDK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3493..3906)
                     /locus_tag="HA513_RS26285"
                     /old_locus_tag="HA513_26285"
     CDS             complement(3493..3906)
                     /locus_tag="HA513_RS26285"
                     /old_locus_tag="HA513_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_166479957.1"
                     /translation="MEKLDYVSKKTEKSRSFLIRESLERFIDELETEYEKREVKIDMNG
                     SFYELLVDECKTPMELTTGARKVAFTMFSDEGKLYVLNSKGNTRPLDEKPANDFFETFK
                     KTGSTSPITYRDSTFNASYFLAATQELMRRGTI"
                     /name="hypothetical protein"
     gene            4102..4395
                     /locus_tag="HA513_RS26290"
                     /old_locus_tag="HA513_26290"
     CDS             4102..4395
                     /locus_tag="HA513_RS26290"
                     /old_locus_tag="HA513_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_137399576.1"
                     /translation="MRTELEFEWDSVKAETNYRKHGIRFEEAALVFDDPFHLSAQDRIE
                     NGEFRWQTIGTVKGCVVVLVAHTTRFEDGTEIIRLISARKAEKNERRRYEHG"
                     /name="BrnT family toxin"
     gene            4382..4684
                     /locus_tag="HA513_RS26295"
                     /old_locus_tag="HA513_26295"
     CDS             4382..4684
                     /locus_tag="HA513_RS26295"
                     /old_locus_tag="HA513_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_166479958.1"
                     /translation="MSMVRYKRSEIPPLTEKRSSELKTLAAEADNHIDYEGIAPLDDAF
                     WKQASRGQFYRPLKTQASVRIDADVMAWLKEPGKGYQTRLNAILREAMLRELHRK"
                     /name="BrnA antitoxin family protein"
ORIGIN
        1 tggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
       61 ctgaacataa aacactatca ataagttgga gtcattaccc gcatcctgtt aatactaata
      121 atgcaataac acttagaggt attgtacttt tcattaaaaa tcctttcatg agaactttta
      181 taagataatg attttactaa aaaataattt tgttataaca aattattcgc caaagtgtga
      241 cactggtctt tgattgcgta gatctcgagg atcgcgagag gaatatcttg agatctatct
      301 tccgtcgtcg taatctcttc tactgtaaac aaaaaaacca cctggggagg tggttttttc
      361 gaaggttaag tcagttgggg aactgtttaa ccgggtaact ggcttatgag gagcgcagtc
      421 accaaaactt gttctttcag tttagcctta actggcgcat aaattcaaga ctcaactcct
      481 ctaatcagtt accagtggct accgccagtg gcgattcgtc gtgtctttcc gggttggatt
      541 caagtgttaa gttaccggaa cggacgtggt agtcggacta aacggggagt tcgtgcagat
      601 agtccagctt ggagcgaacc acctgtcagg aacccagttg cacgggaagg aatgagaaaa
      661 cgccaccacg gagtctacgg aacggcggaa tgcaacgggt aaaccttacg gtgggaacag
      721 gagagcgcac gagggagcca tcagggggaa acgcctggta tctttaagtc ctgtcgggtt
      781 tcgccacttc tgatttgagt accggagatc gtctgatttc tgtgaaactc gtcaggaggg
      841 cgaagcctat ggaaaaacgc cctggcgggg cgtgtccttc tcgtttttgc cctggctttt
      901 ggtcacgctt accgtctacg ttatctgtta cttagaatcc caccgctcgc cgcagactca
      961 tgaccgaacg tagtgagcga atgagcgagg aagcggaata tcatcagatc ctgcatttgg
     1021 tactgccaca cggaggtttt cgtgaacaaa gaacaaaaaa ctgcgctgaa tatggccaaa
     1081 ttcattcagg gtcagtctct gcttttgctg gagaagctca atgccctgga tttcgatact
     1141 gaggctgacc tttgcgagaa gctccatgag gatacggaac agctttaccg tgttttgtca
     1201 gaacgactga aggaatcaga ggatggctac taatggcgaa agtctctatc agtgaagctg
     1261 cgcggcttgt aaatgtcagt cgccctacgc tttacaagat gcttaacaat ggaacgctta
     1321 cttacacctc ggttgtaaag catggaaagc aagtaaaaac cattgatacg tctgagctta
     1381 tacgtgtttt tggttcgctt ggtggtgtaa aggacgatac acaaaacgct gtaagtgttg
     1441 acggtcatgc tacagcgatt aacagtgata ttttacaaga tttacagcat cagattgcgt
     1501 tgttaaagac cgaaaatgac gggcttaaag gggcggtgaa tgccagggat gaacatatca
     1561 gttcacttcg tcaggctatg ttgcttctgg agcataaaca ggagcagcta gcaccgcaga
     1621 tctcttctcc ggcgcactcc ccgtggtgga agttctggaa gtagttctgt cagtgcataa
     1681 ccgccccgta ataccacatc cccaggaatg cgccgcccgt actgacgcac agcaggatca
     1741 gtgcggtgag cagtagcgtc aggaaccggt ctttccataa agcattccct gtacgggctg
     1801 gaacgaccca tccacctgtt aggcgcattt gcttttgaat ttggggttcg gtttttgggc
     1861 acggccgtcg ggcgtaatca tcgggcacag ggtgcgcccg atgattacgc ccgatgattt
     1921 tatcggtgag tggcctcggt gaacgtcctc ggtgaatcgc atgcaccgag gacgttcacc
     1981 gatgaaccgt cttcagggag tcgctaaatc cctttaattc tggcgttccg atggcttgct
     2041 ggacgccgcc atctctacga cacaggatat tattccgctg gacgctgcat aacattctga
     2101 agggcggcca caccgtctcg ctacgcgcga ccaaccccct tacaagccaa tttttacccc
     2161 tgttttttcc tgtgcgtttc ccgctcataa aaaaccagtg gaattgtctg taacgaggtt
     2221 ggtcgcgcgt cgtccttgag gactagctta acggtgtggc gggctttgcc cccaaatccg
     2281 aagtcgccat tgggatggct tgcctcagta ggctgagtca ttcgaattcg gcccccccga
     2341 aggcatgatc ccctgaacgg gtatcacaaa aaacaggtca gacccggttt aacaggagga
     2401 tggctcgcag tggcgaacca tcccccttcc ccaagggtgg ggtcgttaca gcccgtaaag
     2461 ggttcgcttc gccgctacgc gtccttgact ggctttctct cgattctaaa gcagttctaa
     2521 gtccccttga tctggcggcg gtggcttgct catacaagac ttcgtgcgct atggggcttt
     2581 tacgcgcgaa tttccgcatc tgccttaggt aaacccggat aaatccgcat tacggtgttt
     2641 ctttcacgcc agttcgggca ccaggcagtg ccgtgcatag tcatgcatac ggttaaaact
     2701 tatcaggggc gtttttcggc atttcagcca gggataaggc ggtcttttac ggcgaacgcg
     2761 ccggaaccgc actgagcgcg ttttcgcggt gcacgcaatg tggtgttatg gtaaatgagg
     2821 aggcatgaac aaccccgcct tggctggatt ttaattgcct attatacggt tattgcttat
     2881 atttcgtgta tacaaatgta attacatcga gggccgtgta tatggcaact atgaacatcc
     2941 gcatcgatga cgatttaaag gcacgttctg gtgcagcgct tgacgcgtta ggcgttacaa
     3001 cgtccgaagc cgttcgcctt gtcctggagt acgttgcgca gaataaccgc ttgccggtgc
     3061 agatcgcatt actggcagac gaagacgcag acatcatcga actggcgcgc gctcgcctgg
     3121 ctaacccggg caaacggata cgcgtgaaac tcgatgacct atgacattga attttacgaa
     3181 gaggcttttg acgaatggca ccggctggat gccagcgtac gtgaccagtt taaaaagaag
     3241 ttagcaaaac tggtcgaaga cccgcaccgg cccgctaatc ggctgcgtgg gcttgagggc
     3301 tgttacaaaa tcaagctacg cgccgcaggg gtgcgtttag tttataaagt cgaggatggc
     3361 gagttagtga tcctggtcgt ggctgtcggc aaacgggata aaaacgcagt ttacgacacc
     3421 gcccgcgaac gactagtcac catagacaaa tagcccgcga ctgcgggctt tttcattggg
     3481 tttacactta tgctaaatcg tccctctacg catcaactcc tgagttgcgg ctaagaagta
     3541 ggatgcattg aatgtggaat ctcgatatgt gattggtgat gtgcttccag tctttttgaa
     3601 tgtttcaaaa aaatcattgg ctggtttctc atcaagagga cgggtatttc ctttagagtt
     3661 taatacgtag agttttccct catcactgaa catagtgaaa gcaacttttc gcgcaccagt
     3721 tgttagctcc atcggcgttt tacattcatc aaccagtaat tcgtaaaatg aaccattcat
     3781 gtctattttt acctctcttt tttcatattc agtttcgagt tcatcaatga agcgctctag
     3841 ggattctcga ataagaaacg aacggctttt ctctgttttt ttagatacat aatcgagctt
     3901 ttccatgagt tcttccggga gtgtgactgc aatcataatc gcctctaatt ttaacctaat
     3961 gtttcatctg gtttatatat taaagttttt tagtcaagtg tgtttgattg cgtaggttgg
     4021 cgtagccttg aatttacagt tattttttct gctgccctgt ggtattgtct atacttaatt
     4081 gtatatacaa aagtgtattt catgagaaca gagcttgagt ttgaatggga ttcagtcaaa
     4141 gcggagacga actaccgtaa gcatggaatt cgttttgaag aggcagctct ggtatttgat
     4201 gatccatttc atctgtcggc ccaggaccgt atcgagaacg gtgagttcag gtggcagacc
     4261 ataggtacgg tgaaggggtg tgttgtggtt cttgtggcgc atacgacacg gtttgaagat
     4321 ggtacggaaa taatccggct tatcagtgcg cgaaaagcag agaagaatga gaggagacgc
     4381 tatgagcatg gttagatata aacgcagtga aatcccccct ctgacagaga agcgttcatc
     4441 tgaattgaaa actcttgctg ctgaggctga taatcatatt gattacgaag gcatcgctcc
     4501 gctggatgat gcgttctgga agcaggcatc cagaggtcag ttttaccggc cgctgaaaac
     4561 ccaggcatca gtccggattg atgctgatgt catggcgtgg ctgaaagaac ccggaaaagg
     4621 ataccagaca agactcaacg ccattcttcg tgaagcaatg ctgcgggaat tgcatcgtaa
     4681 gtaacttcag acaacaacac ggtcccgcta tatgcgggat ttgttataac cttttttggt
     4741 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     4801 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     4861 caccgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt
     4921 cacttacacc gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg
     4981 cgttggatgc cgggcaaccg cccgcattat gggcgttggc ctcaacacga ttttccgcca
     5041 tttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc
     5101 gtctgcgcgg aaatggacga acagtgggga tacgtcgggg ctaaatcgcg ccagcgctgg
     5161 ctgttttacg cgtatgacag gctccggaag acggttgttg cgcacgtatt cggtgaacgc
     5221 actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg
     5281 atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc
     5341 aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg
     5401 ctggga
//
