LOCUS       NZ_CP050071            13466 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella aerogenes strain 035 plasmid p35_D, complete sequence.
ACCESSION   NZ_CP050071
VERSION     NZ_CP050071.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14372988
            Assembly: GCF_011604725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Nuesch-Inderbinen,M., Stephan,R. and Stevens,M.J.A.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   Nuesch-Inderbinen,M., Stephan,R. and Stevens,M.J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-MAR-2020
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: ILS_035_Kaerogenes
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 05:50:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,267
            CDSs (total)                      :: 5,140
            Genes (coding)                    :: 5,004
            CDSs (with protein)               :: 5,004
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 44 of 136
            Pseudo Genes (incomplete)         :: 95 of 136
            Pseudo Genes (internal stop)      :: 19 of 136
            Pseudo Genes (multiple problems)  :: 19 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050071.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13466
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="035"
                     /isolation_source="water"
                     /db_xref="taxon:548"
                     /plasmid="p35_D"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            join(12787..13466,1..25)
                     /locus_tag="HA513_RS26205"
                     /old_locus_tag="HA513_26205"
     CDS             join(12787..13466,1..25)
                     /locus_tag="HA513_RS26205"
                     /old_locus_tag="HA513_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            341..1852
                     /locus_tag="HA513_RS26210"
                     /old_locus_tag="HA513_26210"
     CDS             341..1852
                     /locus_tag="HA513_RS26210"
                     /old_locus_tag="HA513_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_166479954.1"
                     /translation="MSNSKDQLFNADHLKTGMKERAVKSAGITLAAQGIKLILQLGSVA
                     ILARLLQPSDFGLIAMVTVFTGLALQFMEGGLSMATIQRDQITHAQVSNLFWVNGALGT
                     ALCLLGILISPLVAIIYDEPRLTLVMAAMSLTFLIGGLSVQHDALLRRQMRFRAISVID
                     IVSMAAGIATGIAAALGGLEYWALVISPITTFATKTVMRWLSARWVPSMMSRGSGVRPL
                     LGFGANLTVANFIGYMATNLTPFAIGYIGGAQSLGLFNRANMLTSIPSSQMLPPVMNVL
                     QPTLARVASDPKRLRSTITSIMGKLVLGTMFVTLTMAVLADWIVQLFLGSGWDGAVPIF
                     RMLAVFSLVEPIAGFLAVSLVAIGNAKALLRWKAIILSIFIVSVGIGSFWGAFGVVAAY
                     ALSGVFIRLPGFLYYSSRFLPVTFGEFIKALVPATVCALGAIAALFTLRHFILIESPVA
                     GLAVFIAAAAVIYPALCLLIKPTRRELIEILDLLKLLTSRKLTSK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            1849..3279
                     /locus_tag="HA513_RS26215"
                     /old_locus_tag="HA513_26215"
     CDS             1849..3279
                     /locus_tag="HA513_RS26215"
                     /old_locus_tag="HA513_26215"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="WP_080815631.1"
                     /translation="MTTNQSPQALNDTVIANGYCVGCGACAVPEQSPFKIVMDDYGQYQ
                     SALKDANVFATTQNDYEKLCPFGSGAPNEDEIGKQQFSETCEYNDQVGYYNGVFAGHVT
                     EGDFRNQGSSGGMGTWVLNELLSKGLVDYVVHVKSETAAEDLHNIPFKYQVSESLEETQ
                     QGGKSRYYPIELSEVLKTVRNKPGRYAVVGLPCFIKSIRLLQAQDPVLAERIQYCVGLV
                     CGHLKSAHYAESLAWQMGIPPQDLEGIDFRAKDPSQPANRYSTYAKGPSGEKVVPTNQL
                     FGADWGAGAFKYKACDFCDDVFAETADVVLGDAWLPEYVQDSYGTNVVVTRNQDIRQLI
                     VNARIEGRLEMDDLGVEKAVQSQDAGLRHRKRGIVDRLNFEVEADRWVPSKRLATPDHA
                     KPQLEKEKQMLRVQMRDLSHESFKQAKERSDINVYIKRMTPVYSKYKRLGIPLKSRLKS
                     KLKSFILRTLKIAGYQRR"
                     /name="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
     gene            3276..4412
                     /locus_tag="HA513_RS26220"
                     /old_locus_tag="HA513_26220"
     CDS             3276..4412
                     /locus_tag="HA513_RS26220"
                     /old_locus_tag="HA513_26220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="WP_080815628.1"
                     /translation="MKVAILTQPLGHNYGGLLQAYALQTYLKKLGCEVETLDRRVEGAG
                     RISLKGHALNLARLMLGRIKSLPTESRQAWVLSELAAFRDRRLTMSPRIASEQEIRDYY
                     RDRDFDAFVVGSDQVWRPRYSPSILNFYLDFLNDIKSPAKRIAYSASFGVDDWEYSGEL
                     TEQCKILAEKFDSISVREQSAVALCRAKLGVSAECVVDPTLLLEPADYEPLITECEKNA
                     NGGCVLSYVLDPAPEKRSIADSVGRAVGANVFSIKPEYSLTQVRAKDIAKCRFPSVESW
                     LQAFHDASFVVTDSFHGTVFSILFNKPFIAVGNSARGMARFESLLSQFGLTDRLVESPE
                     EVSPKLVHSQIDWKAVNDKREALASAGRQFLQTQLFGS"
                     /name="polysaccharide pyruvyl transferase family protein"
     gene            4417..5415
                     /locus_tag="HA513_RS26225"
                     /old_locus_tag="HA513_26225"
     CDS             4417..5415
                     /locus_tag="HA513_RS26225"
                     /old_locus_tag="HA513_26225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_050546070.1"
                     /translation="MPVAPKVSVIVPVFNAGAKLLPSIESLLNQTLKDIEIIIVNDAST
                     DNSGSVIDQLAKENTNIVPVHFSENKGVHEARLAGLKKSTAPWIGFLDADDFARPNMFA
                     TLLSAAVDNDVDIVVCGSDRVTEQRKVIAPKLRFRRSEKVDDNVFERFCAFEFGTGMLW
                     NKLFRRSVIEPWFDLHFPWRQSINEDLILNIGCFYNAKSVYLLKDRLYEYVLAEGSVTT
                     DMKKCWAYSETFRAYVLAVTSYQSLGDEAVTKIVDMYRTQLTWGGYLIDSASVLTRYNT
                     GLEEAANYLSQKAPFSLAAISARKPQTVVGARLALVSLFSSVKSKIQAIFG"
                     /name="glycosyltransferase"
     gene            5435..6268
                     /locus_tag="HA513_RS26230"
                     /old_locus_tag="HA513_26230"
     CDS             5435..6268
                     /locus_tag="HA513_RS26230"
                     /old_locus_tag="HA513_26230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="WP_077251481.1"
                     /translation="MSLIVTLTSTSRRLPVLRHTLLSLFDQQCSADRIVLCISKEPYLI
                     DEGIKKLPAWLTSMVEQGQVEINWVENTGPYRKLMPVYRAASDEDWIVTCDDDVIYGPE
                     WLSSLVETAKQHPSAIVCGRARRPAKNRLGRRQSYLNWRLVPLGSSGKDLLPIGIAGVL
                     YRKPLLDHDTMFSEDYKRLAPKQDDLWFNLARQVAGTEVVVSPDTNNHVYPIEAPGALS
                     ATNAATKVSGWDKLFAALFDRLVLKMKSYFGVAVCDNDIAMEQLDRYKRGALKVS"
                     /name="glycosyltransferase"
     gene            complement(6317..9215)
                     /locus_tag="HA513_RS26235"
                     /old_locus_tag="HA513_26235"
                     /pseudo=""
     CDS             complement(6317..9215)
                     /locus_tag="HA513_RS26235"
                     /old_locus_tag="HA513_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            9310..9915
                     /locus_tag="HA513_RS26240"
                     /old_locus_tag="HA513_26240"
     CDS             9310..9915
                     /locus_tag="HA513_RS26240"
                     /old_locus_tag="HA513_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            complement(10324..10515)
                     /locus_tag="HA513_RS26245"
                     /old_locus_tag="HA513_26245"
     CDS             complement(10324..10515)
                     /locus_tag="HA513_RS26245"
                     /old_locus_tag="HA513_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11545..11661
                     /locus_tag="HA513_RS26250"
                     /old_locus_tag="HA513_26250"
     CDS             11545..11661
                     /locus_tag="HA513_RS26250"
                     /old_locus_tag="HA513_26250"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_166479955.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFKEKKAISRTFRRRR"
                     /name="small membrane protein"
     gene            complement(11871..12107)
                     /locus_tag="HA513_RS26255"
                     /old_locus_tag="HA513_26255"
     CDS             complement(11871..12107)
                     /locus_tag="HA513_RS26255"
                     /old_locus_tag="HA513_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            12480..>12722
                     /locus_tag="HA513_RS26680"
                     /pseudo=""
     CDS             12480..>12722
                     /locus_tag="HA513_RS26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(12343..12514)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.356"
                     /coverage="100.0"
                     /evalue="1.61e-48"
     Typing          10683..11103
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="100.0"
                     /evalue="1.95e-162"
     Typing          12481..12723
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.262"
                     /coverage="99.0"
                     /evalue="8.55e-101"
ORIGIN
        1 agtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct
       61 aactttgcaa cagtgccgaa aattctgtgt ttgacatgct taatttgagt ggcgggtatc
      121 agaaatactt gtcttacctt gaggatatgt ggatagttgt agccacattg aggctacaaa
      181 gagaagcgcg gtgtgttatg agtaccgata aatgctgata acccggataa gttcccatat
      241 cggctaaaac ctgactgaca aaacgacgaa gagctctatg tatggatttt gtcattcgaa
      301 atagtttttt aatctgcacc ctaactccca caggtaatcc ttgagcaact ccaaagacca
      361 actcttcaac gccgaccacc tcaaaaccgg catgaaagaa cgcgccgtaa aaagcgcggg
      421 tatcaccctg gccgctcagg gcatcaagct gatcctgcaa ttgggctccg tagccattct
      481 ggcccgtttg ctgcagccgt cagatttcgg cttgattgcc atggtcaccg tgttcaccgg
      541 gctggctctg cagtttatgg aaggtggcct atccatggcg accatccagc gcgaccagat
      601 cacccacgct caggtgtcca acttgttctg ggtaaacggc gccttgggca cggcgttgtg
      661 cctgctgggc atcctgatct cgccgctggt tgccattatc tacgacgaac cccgcctgac
      721 actggtgatg gccgccatga gcctgacctt ccttatcggt gggctatccg tacaacacga
      781 tgctctattg cgccggcaga tgcgctttag agccatctcg gtgatcgaca ttgtgtccat
      841 ggcggcaggc attgctaccg gcatagccgc agctctggga ggccttgaat actgggcgct
      901 agtgatcagc cccattacta cctttgcaac aaaaaccgtc atgcgctggc tgagtgcccg
      961 ctgggttcca tcgatgatga gccgggggtc cggcgtgcgg cctttgttgg gatttggggc
     1021 gaatctgacc gtggcgaatt ttattgggta tatggccaca aacctcaccc cattcgccat
     1081 aggctacata ggtggtgcgc agtcgctggg gttgtttaat cgtgccaata tgctcacatc
     1141 cataccttca agccagatgc tgccgccggt aatgaatgta ttacagccga ctctggcgcg
     1201 ggtggccagt gatccgaagc ggctgcgcag caccatcaca tcgatcatgg gcaagctggt
     1261 gctcggtacg atgtttgtga cgctcaccat ggccgtcttg gcagactgga tcgtccaatt
     1321 gtttctgggc tcgggctggg acggcgcggt tcccatcttt cggatgctgg cagtgttttc
     1381 tttagtagag cccattgcgg gcttcctggc tgtttctctt gtcgcgatag gtaacgccaa
     1441 agcattgctg cgatggaaag cgattatttt gtcgattttt attgtctctg tcggcatcgg
     1501 ctcattttgg ggggcctttg gtgtggtcgc ggcctatgcg ctctctggtg tgttcattcg
     1561 tttgccgggg tttctgtatt actctagccg gttcctgcca gttacttttg gtgagttcat
     1621 caaggcatta gtgcctgcta cagtctgtgc cttgggggcc atcgcagctt tgttcaccct
     1681 tcggcatttt attctgatag aaagcccagt agcaggttta gcagtattca ttgccgctgc
     1741 agcggtgatc tacccggcgc tgtgtttact gattaaaccc acgcggcgtg agctgatcga
     1801 aatcctagac ctactcaaac ttctaacttc caggaaattg acttcaaagt gacgaccaac
     1861 caaagccccc aggcactgaa cgataccgtt atcgcgaacg gctactgtgt aggctgcggt
     1921 gcctgtgccg tgccggaaca aagcccgttc aaaatcgtta tggacgacta cgggcagtat
     1981 caatctgctc tgaaagacgc caatgtattt gcgacaacgc aaaacgacta cgaaaaactc
     2041 tgtccgttcg gttcgggtgc gccgaatgag gacgaaatcg gcaaacaaca attttctgaa
     2101 acctgcgagt acaacgatca ggtaggttat tacaacggag tgttcgccgg gcacgttacc
     2161 gaaggtgatt ttcggaacca gggcagctcc ggtggaatgg gtacctgggt gctcaacgag
     2221 ctgctctcca aagggttggt ggattacgtg gtacacgtta aatccgaaac ggcggccgaa
     2281 gacctgcaca acattccgtt caagtatcag gtcagcgagt ccctcgaaga aacccagcag
     2341 ggtggcaaat cacgctatta cccgattgaa ctgtcagaag tactgaaaac cgttcggaac
     2401 aagcccgggc gttacgccgt tgttggttta ccgtgcttta tcaagagcat cagactgctt
     2461 caggcccaag acccggtact cgccgaacgt atccaatact gcgttggctt ggtgtgcggt
     2521 cacctgaaaa gcgcccacta tgcagagagc ctggcctggc aaatgggtat tcctccacag
     2581 gaccttgagg gcatagattt ccgcgctaaa gatccctcac aacccgccaa ccgctacagc
     2641 acctatgcca agggcccttc tggcgagaag gtagtgccca ccaaccaact gttcggcgca
     2701 gactggggcg caggcgcttt caagtacaag gcctgtgatt tctgtgacga cgtgtttgcc
     2761 gaaaccgccg atgtggtgct gggcgatgcc tggttgccgg agtatgtgca ggatagctat
     2821 ggcaccaacg ttgtggtaac cagaaaccaa gacatccggc agttgattgt gaatgcccgt
     2881 attgaggggc gtttagagat ggacgaccta ggtgttgaaa aggccgtgca gtctcaggat
     2941 gcggggttgc ggcacaggaa gaggggaata gtcgatcggt tgaattttga ggtggaagcc
     3001 gatcgctggg ttcccagtaa gcgtctggcg actcctgacc atgcaaagcc gcagcttgag
     3061 aaagaaaagc aaatgctgcg ggtacagatg agagatcttt ctcatgagag ctttaagcag
     3121 gccaaagaac ggtcagatat aaatgtctat attaaacgaa tgacaccagt ttattcgaaa
     3181 tacaaaaggc ttgggatacc gttgaagagt aggctgaagt caaaactgaa gagtttcatt
     3241 ctacggactc taaaaattgc aggatatcag cgcagatgaa ggttgctatt ctcacccaac
     3301 cccttggcca caactacggt ggcctccttc aagcctacgc cttacaaacc tatctcaaga
     3361 agctaggctg cgaggtagaa acgcttgaca ggcgtgtcga aggcgcaggg cgaatttccc
     3421 tgaaaggcca tgccttaaac cttgccaggc tgatgctggg ccgcattaaa tcattaccga
     3481 ccgaaagccg acaggcttgg gttttgagtg aactggcggc ttttcgagac cgcaggttaa
     3541 ccatgtcgcc ccgaatagcg agtgaacaag aaattcggga ttattatcgc gaccgggatt
     3601 tcgacgcttt cgttgttggc agcgatcagg tttggcggcc tcgctattca cctagcattt
     3661 tgaattttta cctggacttt cttaacgaca tcaaaagccc cgcaaaacgg atcgcttatt
     3721 cggcttcgtt cggggtggac gactgggaat actccggcga actgactgag caatgcaaaa
     3781 tcctggcaga gaagtttgac tcaatctctg tgcgagagca gtcagctgtt gcgttgtgcc
     3841 gggctaagct gggtgtttcg gctgagtgtg ttgttgaccc aactctgtta ctggagccag
     3901 cggactatga gccgttgatt actgagtgtg aaaagaatgc taatggcggc tgtgtgcttt
     3961 cctacgtgct cgacccggct cccgagaagc gttcgatagc cgatagtgta gggcgtgctg
     4021 tgggggccaa cgtgttctcg atcaagcccg agtactcgct cactcaggtg cgggcaaaag
     4081 atatagccaa gtgtcggttc ccaagcgttg aaagttggct tcaagccttt cacgatgcca
     4141 gttttgtcgt gacagactct tttcacggta ctgtgttttc gattttgttc aataagccgt
     4201 tcattgcggt cgggaactcg gccaggggaa tggctcgatt tgaatcgctt ctgtcacagt
     4261 ttgggctgac tgacaggctg gttgaatctc cagaggaggt atcgccaaag ctagtccata
     4321 gccaaattga ttggaaggct gttaacgata agcgggaagc cttggctagt gctggccgtc
     4381 aatttctaca aactcaactt ttcggtagct gattgaatgc ctgttgcacc caaagtctct
     4441 gtcatcgttc cggtattcaa tgccggggct aagttgctcc ccagcatcga atcactgctc
     4501 aaccagacct tgaaagacat cgagatcatc atagtcaacg atgcttctac cgataactcc
     4561 ggctcagtta ttgatcagtt ggctaaagag aacaccaata ttgttccggt gcatttttcc
     4621 gagaataaag gcgttcatga ggcgcgactg gctggcttga agaaatccac agcgccctgg
     4681 atcgggtttt tggatgctga cgattttgcg cgaccaaaca tgtttgcgac attgctttct
     4741 gcagcggtag ataacgatgt tgatatcgta gtctgtggct cagacagagt tacagagcag
     4801 cgtaaggtga ttgcaccaaa gttgcggttt cggcgttcgg agaaggttga cgacaacgtt
     4861 tttgagcggt tttgtgcgtt tgagtttggt accggaatgc tgtggaacaa gttgttcagg
     4921 cgttcggtga ttgagccatg gtttgatctg cactttccct ggcggcaaag cattaatgaa
     4981 gatcttattc taaatattgg ttgcttctat aacgcaaagt ctgtgtattt gttgaaagac
     5041 cggctttatg agtacgtatt ggcagaaggt agtgtcacaa ctgacatgaa aaaatgttgg
     5101 gcctattctg aaacatttag agcctatgtg ttggccgtaa ctagctatca aagcctagga
     5161 gatgaggccg taacgaaaat agtagatatg tatcgaactc agttgacttg gggaggttat
     5221 ctgattgata gcgcctcggt gttgactcga tacaataccg ggctcgaaga ggctgctaat
     5281 tatttatctc agaaggctcc gttttcttta gccgcaatat cggcgaggaa acctcaaacg
     5341 gtggttggcg cacgcttggc tctggtgtcc ctttttagtt cagttaagtc aaaaattcag
     5401 gcgattttcg gctgatatct ttaataggtc ttctatgtca ttgattgtca ctctaacttc
     5461 tacatctcgt cggcttcctg ttctcaggca caccttgtta tctcttttcg atcagcaatg
     5521 cagtgcagac cggatagtgc tttgtatttc aaaagagcct tacctgattg acgagggaat
     5581 taaaaaactg ccagcctggt tgacgtcgat ggtagagcag gggcaggttg aaattaattg
     5641 ggttgaaaat accggccctt atcgaaagct catgccggta taccgggctg caagcgatga
     5701 ggattggatt gtaacctgtg acgatgacgt tatttacggg cccgaatggc tatcttccct
     5761 ggtagagact gctaagcagc acccttcagc aatcgtatgc ggcagagcac ggcgaccggc
     5821 caagaatcgg cttggtcgta ggcaaagcta tttgaattgg cggttggtgc ctttgggctc
     5881 tagcggaaag gaccttttgc caattgggat cgctggtgtg ctgtatcgga agcccttgtt
     5941 agaccatgac accatgttta gcgaagacta caagagactt gcgccaaaac aggatgatct
     6001 ttggtttaat ctggccaggc aagtggccgg tacagaggtt gttgtgtcgc cggacaccaa
     6061 taaccacgtc tacccgatag aagcgccggg cgcattatca gcgaccaatg cagcgacgaa
     6121 ggtttcgggg tgggataaat tgtttgccgc cttgtttgat cgcctggttt tgaaaatgaa
     6181 gtcttatttt ggtgttgctg tttgtgacaa cgacattgct atggagcagc ttgatcggta
     6241 caagagaggg gcactaaagg tctcctgatc tgttggcgat gttggggtcg gttccggctg
     6301 agggcgaaat gacaccctaa gctttcggtt ccttgggcca aagatattcg ccagtcagta
     6361 gaatgtgcgc ccagcccaat ggggatatgt ggggaagaaa ttcaggggga acatccaacc
     6421 cttcgttccg ccgctccgtg acggcatgac caagatggac ggtattccag taaatgatca
     6481 ccgcagtcaa taaattgagc ccagcgattc ggtagtgctg cccctctgtc gtgcgatcgc
     6541 gaatttcccc cctgcctccc gatacggagc gcatttttga gcgcatggtg ggcctctccc
     6601 ttgttaagac cgatctgagc acgccgctgc atgtccgtat ccaggatcca ctcaataatg
     6661 aaaagggtcc gttcaatacg accaacttca cgaagcgcag ctgcaaggtt gttttgtcgt
     6721 gggtaagaag cgagcttgcg caggagttgg ctgggcctga ttttgccagc ggtcatcgtc
     6781 gcggcacaac ggaaaatatc aggccagttc gcaacgataa gatcctcccg ggcttttcca
     6841 cctaccaact tgcgtaactc cctgggggtc gtatcgggat taaatacgta caaccacttc
     6901 gatggcagat ccctgattcg cagaacgaga ttgtagccga gcaggctact ggctccgaac
     6961 aaatggtcgg tgaatcctgc tgtatcggca tactgttcgc gaacatggcg accgacctcg
     7021 ttcatcagta gtccatcgag aatatacggt gcctcgctca cggtcgccgg gatcgactga
     7081 caagcgaatg gcgcgaactg gtcgcttacg tgagtatacg ctttgaggcc gggaacagaa
     7141 ccatatttgg cattgaccat gttcatggct tcgccatgcc gcgctgtcgg gaaaaactga
     7201 ccatcgctca atgctgacgt gcccatcccc cagacgcgtg acatcggcag tttaccctgc
     7261 gcggccacca caattgccaa tgcctggttc atggcttcgc tttcaacatg ccagcgggca
     7321 aggcgtgaga gctgccagta atcatgcgtg tttgtagctt ccgccatctt acgcaggccc
     7381 agattgagcc cttcagcgag caggacgttg agcagaccga tccggtcgcg acatggagcc
     7441 ccggttctca gatgggtaaa cgcatctgtg aaaccaaggg ctgcatcaac ttcaagcagc
     7501 atgtcggtaa tccgaacgga cggcattcgg cgatacagat ccagtatgag tgcctcggca
     7561 ccatccggca cgtctgctgt caacctgtcg atccgcaacg ttccatcttc tatgctaccg
     7621 tgcggaatag tgccgttacg ggcagcccgg gccagccgct taagagcgat cgtgagtcgc
     7681 gcctttctgt ctgccagcca atcctgtggg ttggaaggca cggccagttt tgcattttcc
     7741 tgcgccgcga tcatcggcac cagtacctgc ttgaggtcac catagcggcg cgaatgagcg
     7801 agccagacat ctccggaacg aaaagcatcc cggaggtgaa agagtaccgc cacttcccaa
     7861 agacgggtat ctcctttttc ctgagctcgt aaatgacggt tccatttgga gctgggccgc
     7921 aggaaacgcc tttctggcga tgcaacacct ttcatctctc cgatcgacaa agctgctgct
     7981 accaatggtc cggcgaccgg cgcggcttcg agcttcagac agcgcaacat gcggggcgca
     8041 taacgacgaa agcgatggta tccctgcccg acatatgcaa gaggctcatc ggctagcgtg
     8101 ttgctgagtt gagtccctgt cgctaccagt tgagcgagcc ggtcccatgc aaccgaactg
     8161 gcgacagcca tctccagcgg ggttccgtca ctgcgggcct caagcaacga agctcccagc
     8221 gcggtgaagg tacggatcgt atccgtgagt gtggctttag agccggaaat tgtttcgtca
     8281 tgctggcgct tcgcttcccg ccaggttttt cctacgatcc tgtcatgggt ttcgactatg
     8341 gcatcagcaa tcgccgcttc ccactccaca acacagacgg caaggatcgc ccagcggcgg
     8401 tccgaagtga tgtcacgcaa accgtcggtg aagtagcgtt caccctgccg acgcagccgg
     8461 gcaatgcgat gggcaggtat gctggccaaa gcactatgat tgatattcag ggtacgcaga
     8521 aattcgagcc tgtcgagcaa acggttagca gcagccgagt tgttaccaac ctcgaagttg
     8581 cgaagccaga tgaaacgact gatattgccg gcgagcattt cactcagaag tttgtccagg
     8641 tgatcgcgaa catccgctgt taaattttcc gcaatccgcg tttcaatccg ccgcacagcg
     8701 gcgaccagag catccgcgca caagcgctcg attgtcgata ctgcgggcag aatggtggaa
     8761 gtttcccgac accgcacaat aaaacgatga gcaagatcct cgtttgatct ggcatcttcg
     8821 gcctggccga aagtccactc ccgcagatca cgggcaccac ggcccgtgaa ggtcttgtag
     8881 ccgtaaattt cgcgcagcgt gtccatgtgc tgctgacggg tttggcgccg tgtggcataa
     8941 gtgagaagcg catcagccgg aactccaagc tgagcaccga cgaaggaaag gacttcacgc
     9001 gggatcatct caccaggagc cagtgcacgg cccggatatc gtaaggcaca aagttgcagg
     9061 gcaaagccaa tcctgttttc cggtctgcgg cgctgcctaa tgttttccag gtcatcatcg
     9121 cccagcgtgt agaacttcag tagcgacagt tcgtccgtgg gcagatcgaa cagcgctgct
     9181 cgctgccgtt cggtgaaaat atggcgtcgt gacatacaaa ttcgtccctt ttgaagtata
     9241 gtctgttttg gacaacagcc agcccatata aatcagggcg ttccgataca aaaatccagg
     9301 agggttcaat tgggacatcg tgccgccatt tactgccggg tttcaacagc ggatcagtct
     9361 tgtgaacgcc aggaatttga tctgcgagcc ttcgccggcc gtgccggcta cgacgtggtg
     9421 ggaatattta aggaaacagg ttcaggaact aaactcgacc gggccgagcg aaagaaagtc
     9481 ctggcgcttg cccagtccag acaaattgat gcaatcctgg tcactgagct ttcccggtgg
     9541 gggcgctcga cgctcgatct gctcaatacg ctacgtgaac tggagaactg gaaggtttcc
     9601 gtgatagcca tgaatggaat ggcgttcgat ctttcgtcgc cgtatggacg aatgctggcg
     9661 acgtttcttt ccggcattgc ggagtttgag cgggatctca tcagcgagcg ggtcaagtca
     9721 ggccttgctg ttgcgaaggc acgtggtaag aggcttggtc gtcaggccgg agtgcgacca
     9781 aaatcagacc gacttttgcc taaggtggtt gcgatgaggg ccgagggacg cagctatcgc
     9841 tggatcgcac gcgagctcgg tatcagcaag aataccgtcg ctgacatcgt gcaacgacac
     9901 agagctaacg cttagggtgt catttcgccc tcagccggaa ccgaccccta aaagtgacgt
     9961 atgagctgaa ggaatgatgt aaaagtgttg taaaagtgat gtatgaactg aaggagcgat
    10021 gtaattctgt agtcaggaag actaacccgt tcatgcaggc atgaacgata cagtgaggaa
    10081 cgcttttttg tcacgatctc cccttaacct gcgtaaatca cccgccatgg ttgattattt
    10141 ttcaacctca aaatggtcat acacctaacg cagctgcgat tttcatcgca acttcatgaa
    10201 aaataaataa aaaagaaaaa atacagggat tctgggtatt accggggtaa taatgttaag
    10261 cgccaggaca atgaaaatta aggcgatgcc ggtattactc cggtaatacc cagaacggag
    10321 ccattacgca ccgtcctgct gctcattcag gcgcgctgac aggcttcggt aaaggcgttc
    10381 cgcatcctca tggagtttct cgcagaaatc agcctcatga tccagatcca gctcattaag
    10441 cctgtccaga agaagcaggg actgatcctg gatgaattta gccatgttaa cggcagtctt
    10501 ttgttgtttg ttcatgaagg ccccggcgcg aaagtgctta ctgtacgttc tgatggtttt
    10561 ccgcttcctc gctcattcgc tcactccgtt cggtcatgag tctgcggcga gcggagggag
    10621 gataagcaga taacgtaacg gtaagcataa cgaaagctaa ggacaagggc gaaaaggcca
    10681 cgccccggcg gggcgttttt ccataggctc cgcccccctg acgagcatca cagaatctga
    10741 cgctcaaatc agcggtggcg aaacccgaca ggactataaa gatcccaggc gtttccccct
    10801 ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
    10861 ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggaagg cagttcgctc
    10921 caagctggac tgtatgcacg aaccccccgt tcagtccgac taccacgctc ttttcggtaa
    10981 ctatcgtctt gagtccaacc cgaaaaggca cgacaaatcg ccattggcgg tagccactgg
    11041 taaaggggtg tacaagagat aactggtgca tagttcttga agttgaagcc taactacggc
    11101 taaactgaaa ggacgtattt ggtggcagcg gtcttgtaca ccctgttacc acggttcaga
    11161 agttccccaa cttgctgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgtttaca
    11221 gagcaagaga ttacgacgag gctgaatgaa tctcaagatt tcatttagcc tgccacccga
    11281 tcattcaaag ttttggcgta gttctgaccc gctccccgtg atttgttaca tacccaccac
    11341 agggcattag tttttttatt tttttgatga tatgtacaaa atcagtggtt aggaataatc
    11401 ttagtagcct taacaaacac atacaaaaac agtggttatg tttcatctca ccacaataag
    11461 taacgacagt atcatgctaa aaaaagatga tattatcttt atgaaatcat aacaggctgt
    11521 ttttattaaa ttaatcaggc acatatggac tggatttcac ttgctatcgc cattgctcta
    11581 cttgttgtag ctgtctactc aggaatctca tacttcaaag aaaaaaaagc aatcagtcgt
    11641 acatttaggc gtaggcgata gcgccctatt taccataatc ccttcttacg cgcaccgcga
    11701 aaacggcctc agggcgtttc tgcgccttca gccacaaaag agcacgtctt acccccagaa
    11761 aaccgcccgg agtcgctctg gtgagtttta cgggggtgaa tgactatgca ccgtagtgaa
    11821 taccacgctg tttcgccctg aactccccac aaaaccgacg aaagtggcaa tcagcgacgc
    11881 ggcgcccgcg gacgcgacca ggtaaattcc gcctgctgcc gctcctcctc acgcgcagat
    11941 cgctgccgtt ctgcctggcg ctgcgattcg atctgtgcac gagccctgtt aaggacaatg
    12001 tgcctgttgg gctgattcag cttcttgcct gacgctctct cagcggcggc aacagcatcg
    12061 tcgagaaatc ttttctcggc gggcgtcagg tttatcagtc gtttcatggc gatctccggt
    12121 cagaataact ctgcacagtc tgccagacac ggcgaacctc atgaagaacc tgcagccggg
    12181 aacagccctg agaaacggtg aacgggaacg gattttaatc cggtgccgtg aagcgttggc
    12241 ggggtatcgg ggcgcagccc tgagatatcg ttttggtgcc ggcatcgtga tggcggtact
    12301 ccaaaaacac atcttgtccc gctttcagtt tctgaatttg cgggttccgg gcgcagcgcc
    12361 ggaccagtca cgtagcgcta gcggagtgta tactggctta gtatgttggc atgagtgcga
    12421 taatcaggaa gcgccttatg cggcagaaga aaaatgctgc agcagagcgt aagcagaata
    12481 tgcgatacag aagatattcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    12541 tgcggcgagc ggtgccggct tacgggcggg gcggaaattt ccaggaagat accaggaaga
    12601 aacttaacag ggaagcgatg aggccgcggc gaagccgttt ttccataggc tccgcccccc
    12661 tgacaagcat cacaaaatct gacgctcaaa tcagtggcgg cgaaacccga caggactata
    12721 aagggcactg ttgcaaatag tcggtggtga taaacttatc atcccctttt gctgatggag
    12781 ctgcacatga acccattcaa aggccggcat tttcagcgtg acatcattct gtgggccgta
    12841 cgctggtact gcaaatacgg catcagttac cgtgagctgc aggagatgct ggctgaacgc
    12901 ggagtgaatg tcgatcactc cacgatttac cgctgggttc agcgttatgc gcctgaaatg
    12961 gaaaaacggc tgcgctggta ctggcgtaac ccttccgatc tttgcccgtg gcacatggat
    13021 gaaacctacg tgaaggtcaa tggccgctgg gcgtatctgt accgggccgt cgacagccgg
    13081 ggccgcactg tcgattttta tctctcctcc cgtcgtaaca gcaaagctgc ataccggttt
    13141 ctgggtaaaa tcctcaacaa cgtgaagaag tggcagatcc cgcgattcat caacacggat
    13201 aaagcgcccg cctatggtcg cgcgcttgct ctgctcaaac gcgaaggccg gtgcccgtct
    13261 gacgttgaac accgacagat taagtaccgg aacaacgtga ttgaatgcga tcatggcaaa
    13321 ctgaaacgga taatcggcgc cacgctggga tttaaatcca tgaagacggc ttacgccacc
    13381 atcaaaggta ttgaggtgat gcgtgcacta cgcaaaggcc aggcctcagc attttattat
    13441 ggtgatcccc tgggcgaaat gcgcct
//
