LOCUS       NZ_CP050035             2389 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain 33-6 plasmid pCD33-6-4, complete sequence.
ACCESSION   NZ_CP050035
VERSION     NZ_CP050035.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14278028
            Assembly: GCF_014075915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Lv,H. and Cui,Q.
  TITLE     Identification of novel plasmids containing the tigecycline
            resistance gene tet(X4) in Escherichia coli isolated from retail
            chicken meat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Lv,H. and Cui,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) College of Veterinary Medicine, China
            Agricutural University, Yuanmingyuan West Road, Beijing 100000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.3.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 04:49:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,266
            CDSs (with protein)               :: 4,266
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 50 of 217
            Pseudo Genes (incomplete)         :: 155 of 217
            Pseudo Genes (internal stop)      :: 39 of 217
            Pseudo Genes (multiple problems)  :: 28 of 217
            Pseudo Genes (short protein)      :: 4 of 217
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050035.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="33-6"
                     /host="broiler chicken"
                     /db_xref="taxon:562"
                     /plasmid="pCD33-6-4"
                     /geo_loc_name="China:Sichuan"
                     /collection_date="2019-05-11"
     gene            complement(372..572)
                     /locus_tag="G9P48_RS22740"
                     /old_locus_tag="G9P48_22775"
     CDS             complement(372..572)
                     /locus_tag="G9P48_RS22740"
                     /old_locus_tag="G9P48_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_023139298.1"
                     /translation="MARSPDPRQRTEGFRKAEASLRLEGMDPAGTPLYENIKERVISGD
                     ITYEQGRAEILAHYQKRANGH"
                     /name="antitoxin VbhA family protein"
     gene            1548..1703
                     /locus_tag="G9P48_RS22745"
                     /old_locus_tag="G9P48_22790"
     CDS             1548..1703
                     /locus_tag="G9P48_RS22745"
                     /old_locus_tag="G9P48_22790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152913171.1"
                     /translation="MNSKQKCSHSNLEKEYYLGSATGDYKCLDCGEVGFGKDWPEKERS
                     KNDNEE"
                     /name="hypothetical protein"
     gene            complement(1731..2144)
                     /locus_tag="G9P48_RS22750"
                     /old_locus_tag="G9P48_22795"
     CDS             complement(1731..2144)
                     /locus_tag="G9P48_RS22750"
                     /old_locus_tag="G9P48_22795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182176663.1"
                     /translation="MKIIKWASLSGVLLISACGVSQSEVNSANYGSKPIDNDAKVIGML
                     KESLKDPDSLKVISITTPARGFSKYGFGSTEHGWFTTITYNAKNSYGGYVGAKSITFVY
                     LNGDYKVAVPMSNGERVMLDKVSFSCKDNCQTK"
                     /name="hypothetical protein"
     Typing          967..1477
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.39"
                     /coverage="99.0"
                     /evalue="1.29e-173"
ORIGIN
        1 tgaaagcctg ctttccggcg cataactccc ccgataacgg tgaacgggtg cacagtttac
       61 tgtgcgaccg tgaggcgttg gccaggccgt caggcatggc agaatttact accaagcagg
      121 aggtcggatt taacgtggat aatcagcacg cagtgatgaa cgggattcgg aaggggaacc
      181 cttgcggcgc ggcgatgtat acatcgctaa cgtgccttac tcttttcaca gcagagggta
      241 gcggtagcaa acaagtagca aacagatagc aaacaagtag caaacagata gcaaatacag
      301 gaaaaccctg gccggagaga ggccacgcct gtttttttga ggaagcgatg gtacctagtt
      361 tcctgtccgt gtcagtggcc gttcgcacgc ttctggtaat gagccagaat ttctgcccgt
      421 ccctgttcgt aagtgatatc cccggaaata acccgctcct tgatattttc gtacagcggc
      481 gttccggcgg ggtccatgcc ttcaagcctc agcgacgctt ccgctttgcg gaaaccctca
      541 gtacgttgtc gtggatcggg ggacctggcc atcgatgttc ctttgagctg taagcgaata
      601 aggcaggcgg taaagtaggc ctactttaca tatcctgccc gtactctgcc gttgtttatc
      661 cgtgcagacg aatgtgtaaa ccggaaggaa agtattgcag gaaactgtgt aatccggcgg
      721 taaagagagg gtacggcgcg acacgagccc aatcaggcat cgcagatctt ccgcttcctc
      781 gctcactgac tcgctacgct cggccgttcg gctgcggcga gcggtaccgg cagtccacct
      841 ggagttaccc acagattcgg gggtagcgga aggagaaaga ggggaacaga aaaggccgcg
      901 gcgaagccgt ttttccatag gcttcgcccc cctgacaagc atcacagaat ctgacgctca
      961 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
     1021 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1081 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1141 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg gtaactatcg
     1201 tcttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca ctggtaacag
     1261 gtttgtgcga gagatgtgtt cagagttctt gaagtggcgg cctgactgag gctacactgg
     1321 aaggacagat ttggtgacat gtctcgcaca agactgttac cacggttaga gaatctgcca
     1381 ggattaacta accttcgaaa aaccacctgc cagggtggtt ttttcgttta cagtcatgaa
     1441 gattacgcgt agaaaaaaag gatctcaaga agatctttcc atcatttaac aatgtcggta
     1501 tagataaaaa tcattcacga ataaatgaat atccaaaggt gactgtagtg aacagcaaac
     1561 aaaaatgttc tcattcaaat ctagaaaaag aatactactt gggtagcgcc acaggcgatt
     1621 ataaatgttt ggattgcggg gaggttggtt ttgggaagga ctggccagaa aaagaaagaa
     1681 gtaaaaatga taacgaagaa taaaaaaggc cgctattgcg acctttttta tcatttagtt
     1741 tggcaattgt ctttacatga aaatgacact ttatctaaca taaccctctc accattagac
     1801 attggcactg ccactttata atctccgttt aagtaaacga aagtaatgga cttagcacca
     1861 acataaccgc cataagagtt ttttgcatta tatgtgatag tagtgaacca gccatgctct
     1921 gtagagccaa agccatactt agaaaaacct ctggcggggg tggttattga tataactttt
     1981 agagaatcag gatcttttag actctcttta agcataccaa tcactttcgc atcattatct
     2041 attggttttg aaccataatt agcactattt acttcgctct gagaaacacc acaagcagat
     2101 attaaaagaa caccagacag tgaagcccac ttaattattt tcatatatat ccttgtcatt
     2161 ttccaaacaa ctataggaaa tccactataa tagacattta tagatagatt taccataaca
     2221 tccgttgcgt gcaccatctc agacgccctc agggcatttc tgcgccctcc tgcttatttc
     2281 cggcatcttt ccctgtccat atcgtgaaaa aacgcgcctg atgatttctg cgcggctgca
     2341 tggctatgca cgcaggtgaa tggcggtcga tttggggtgg atgctggca
//
