LOCUS       NZ_CP050032            30707 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain 33-6 plasmid pCD33-6-1, complete sequence.
ACCESSION   NZ_CP050032
VERSION     NZ_CP050032.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14278028
            Assembly: GCF_014075915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30707)
  AUTHORS   Lv,H. and Cui,Q.
  TITLE     Identification of novel plasmids containing the tigecycline
            resistance gene tet(X4) in Escherichia coli isolated from retail
            chicken meat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 30707)
  AUTHORS   Lv,H. and Cui,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) College of Veterinary Medicine, China
            Agricutural University, Yuanmingyuan West Road, Beijing 100000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.3.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 04:49:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,266
            CDSs (with protein)               :: 4,266
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 50 of 217
            Pseudo Genes (incomplete)         :: 155 of 217
            Pseudo Genes (internal stop)      :: 39 of 217
            Pseudo Genes (multiple problems)  :: 28 of 217
            Pseudo Genes (short protein)      :: 4 of 217
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP050032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..30707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="33-6"
                     /host="broiler chicken"
                     /db_xref="taxon:562"
                     /plasmid="pCD33-6-1"
                     /geo_loc_name="China:Sichuan"
                     /collection_date="2019-05-11"
     gene            54..1382
                     /locus_tag="G9P48_RS22540"
                     /old_locus_tag="G9P48_22565"
     CDS             54..1382
                     /locus_tag="G9P48_RS22540"
                     /old_locus_tag="G9P48_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="WP_226201773.1"
                     /translation="MTIQKSGETRNDIGLSVSESKLLLNTVQQSVVQLQADEYTQHHIR
                     CPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCRCEESDTKTVSLLSDWAGDYSHPA
                     LKYIETRWASMISYEMTTRLLKDILPVGHSLNASTVRNHLCQVAQRLDAEAEAHSGFLS
                     GCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRNFEIIAGKSFSVGAPADTRRFGFVQK
                     DDCHPERRLMTHLSAQGMQANQQIFFLSDGADNLRDLQFGMYPESTHVLDWFHITMRLK
                     VLMQYARGLLVSDPEAGSKVLALLESIKRYLWHGNVVAALEHIDNCVMYCDDPELSYPS
                     LKSLQKHLDEMYTYIRNNKMMIPNYGEMRRYGEPVSTAFVESTINEVIARRMAKKQQMQ
                     WSRKGAHYLLQTRTAVLNNELQDKFVCWYPGFQSDGKGPAMAA"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            <1415..1843
                     /locus_tag="G9P48_RS22545"
                     /old_locus_tag="G9P48_22570"
                     /pseudo=""
     CDS             <1415..1843
                     /locus_tag="G9P48_RS22545"
                     /old_locus_tag="G9P48_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(2162..2818)
                     /locus_tag="G9P48_RS22550"
                     /old_locus_tag="G9P48_22575"
     CDS             complement(2162..2818)
                     /locus_tag="G9P48_RS22550"
                     /old_locus_tag="G9P48_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            3213..>4228
                     /locus_tag="G9P48_RS22555"
                     /old_locus_tag="G9P48_22580"
                     /pseudo=""
     CDS             3213..>4228
                     /locus_tag="G9P48_RS22555"
                     /old_locus_tag="G9P48_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(4271..7276)
                     /locus_tag="G9P48_RS22560"
                     /old_locus_tag="G9P48_22585"
     CDS             complement(4271..7276)
                     /locus_tag="G9P48_RS22560"
                     /old_locus_tag="G9P48_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            7440..7997
                     /locus_tag="G9P48_RS22565"
                     /old_locus_tag="G9P48_22590"
     CDS             7440..7997
                     /locus_tag="G9P48_RS22565"
                     /old_locus_tag="G9P48_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            8180..9040
                     /locus_tag="G9P48_RS22570"
                     /old_locus_tag="G9P48_22595"
     CDS             8180..9040
                     /locus_tag="G9P48_RS22570"
                     /old_locus_tag="G9P48_22595"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(9298..10089)
                     /gene="sul3"
                     /locus_tag="G9P48_RS22575"
                     /old_locus_tag="G9P48_22600"
     ARG             complement(9302..10089)
                     /name="sul3"
                     /gene="sul3"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul3"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(10558..10803)
                     /locus_tag="G9P48_RS22580"
                     /old_locus_tag="G9P48_22605"
     CDS             complement(10558..10803)
                     /locus_tag="G9P48_RS22580"
                     /old_locus_tag="G9P48_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="WP_001354008.1"
                     /translation="MKIEIMEYNPDWTKNFEEEKIKLLHFFGSHAVAIEHIGSTAIPNQ
                     RAKPVIDIFIGVSPFAELPFISAFLMQRSITTLRQI"
                     /name="GrpB family protein"
     gene            complement(10841..11704)
                     /locus_tag="G9P48_RS22585"
                     /old_locus_tag="G9P48_22610"
     CDS             complement(10841..11704)
                     /locus_tag="G9P48_RS22585"
                     /old_locus_tag="G9P48_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_000612791.1"
                     /translation="MIPNSENKRVWFITGASKGLGYAFTCAALKAGDKVVAVARTIDNL
                     AKLEETYQESLLPLNLDVTDREAVFSTVETAVKHFGRLDIVVNNAGIMTMGMIEELNES
                     DARKLMDTNFFGALWVCQAVMPYLRSQRSGHIIQITSIGAIISGPMSGIYSASKFALEG
                     MSEALAKEAEHFGVKLTMVEPGGYWTDLYTSMSYSNPLDSYGTLRDELAKQYSEDSVDS
                     DPSLAAEALMKLVASNNPPLRLILGSMVYDLAMDTLKARMATWEEWEAVSRASEKAIPA
                     PERYGV"
                     /name="SDR family oxidoreductase"
     gene            complement(11833..>12102)
                     /locus_tag="G9P48_RS22590"
                     /old_locus_tag="G9P48_22615"
                     /pseudo=""
     CDS             complement(11833..>12102)
                     /locus_tag="G9P48_RS22590"
                     /old_locus_tag="G9P48_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(12154..12858)
                     /locus_tag="G9P48_RS22595"
                     /old_locus_tag="G9P48_22620"
     CDS             complement(12154..12858)
                     /locus_tag="G9P48_RS22595"
                     /old_locus_tag="G9P48_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(12928..13401)
                     /gene="dfrA14"
                     /locus_tag="G9P48_RS22600"
                     /old_locus_tag="G9P48_22625"
     ARG             complement(12932..13401)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            13557..>14354
                     /gene="intI1"
                     /locus_tag="G9P48_RS22605"
                     /old_locus_tag="G9P48_22630"
                     /pseudo=""
     CDS             13557..>14354
                     /gene="intI1"
                     /locus_tag="G9P48_RS22605"
                     /old_locus_tag="G9P48_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /name="intI1"
     gene            complement(14407..15111)
                     /locus_tag="G9P48_RS22610"
                     /old_locus_tag="G9P48_22635"
     CDS             complement(14407..15111)
                     /locus_tag="G9P48_RS22610"
                     /old_locus_tag="G9P48_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <15177..16691
                     /locus_tag="G9P48_RS22615"
                     /old_locus_tag="G9P48_22640"
                     /pseudo=""
     CDS             <15177..16691
                     /locus_tag="G9P48_RS22615"
                     /old_locus_tag="G9P48_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            complement(16790..17428)
                     /locus_tag="G9P48_RS22620"
                     /old_locus_tag="G9P48_22645"
     CDS             complement(16790..17428)
                     /locus_tag="G9P48_RS22620"
                     /old_locus_tag="G9P48_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_024245155.1"
                     /translation="MTQTVPKGFVRAYLRASTSEQDASRALAAIDSFAEERGLNICNYY
                     IENESGSRLDRPELFRLLKDCRHNDILLIEDVDRLSRLTGEDWNSLKKLIRQKDIRVMA
                     INVPTTWIASGSHDFDSRMFAAINDMLLDMLAAVARRDYEQRRERQRQGISKAQKEGRY
                     KGRQANKERYDAINRLLDSGSSWSQVQKIMACSRGTISSAVKSRKLNAI"
                     /name="recombinase family protein"
     gene            17809..18471
                     /locus_tag="G9P48_RS22625"
                     /old_locus_tag="G9P48_22650"
     CDS             17809..18471
                     /locus_tag="G9P48_RS22625"
                     /old_locus_tag="G9P48_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            18569..18850
                     /locus_tag="G9P48_RS22630"
                     /old_locus_tag="G9P48_22655"
     CDS             18569..18850
                     /locus_tag="G9P48_RS22630"
                     /old_locus_tag="G9P48_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     gene            18875..19852
                     /locus_tag="G9P48_RS22635"
                     /old_locus_tag="G9P48_22660"
     CDS             18875..19852
                     /locus_tag="G9P48_RS22635"
                     /old_locus_tag="G9P48_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_024245156.1"
                     /translation="MRYLTELICDLYRLTDHHKLTTATFKNLADIKLFKPTADADTILQ
                     LENIFSEYSLRYIDKDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVING
                     SWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRPPINVKYYSWFDRYVAS
                     NSGGSHHAAMVVYQSVRDGLEYKREAVIKQLSIDPGTVEILDKNYYSFIFQIKTLNKET
                     RIYTSEYEFTDALKDFGQNRHTILKPVNYVSNIKLVFIPKDALKTNSETFRNWFHSAIS
                     CGKIISLPDYLKNPAHYHTLHYSHEVDSITLGDPSRKFKLKGDS"
                     /name="DUF6685 family protein"
     gene            complement(19849..20205)
                     /gene="ddp1"
                     /locus_tag="G9P48_RS22640"
                     /old_locus_tag="G9P48_22665"
     CDS             complement(19849..20205)
                     /gene="ddp1"
                     /locus_tag="G9P48_RS22640"
                     /old_locus_tag="G9P48_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000456533.1"
                     /translation="MEYQVREFINEKYTKAVNILKDNLKENYHVFYGVRLIEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKILDASLLEGTNIVVLECITLA
                     DLLTNDNIDFLYKS"
                     /name="ddp1"
     gene            complement(20342..20788)
                     /locus_tag="G9P48_RS22645"
                     /old_locus_tag="G9P48_22670"
                     /pseudo=""
     CDS             complement(20342..20788)
                     /locus_tag="G9P48_RS22645"
                     /old_locus_tag="G9P48_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(20828..21664)
                     /locus_tag="G9P48_RS22650"
                     /old_locus_tag="G9P48_22675"
     CDS             complement(20828..21664)
                     /locus_tag="G9P48_RS22650"
                     /old_locus_tag="G9P48_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001050931.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="RepB family plasmid replication initiator protein"
     gene            22898..23149
                     /locus_tag="G9P48_RS22655"
                     /old_locus_tag="G9P48_22680"
     CDS             22898..23149
                     /locus_tag="G9P48_RS22655"
                     /old_locus_tag="G9P48_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            23139..23420
                     /locus_tag="G9P48_RS22660"
                     /old_locus_tag="G9P48_22685"
     CDS             23139..23420
                     /locus_tag="G9P48_RS22660"
                     /old_locus_tag="G9P48_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            23495..24188
                     /locus_tag="G9P48_RS22665"
                     /old_locus_tag="G9P48_22690"
                     /pseudo=""
     CDS             23495..24188
                     /locus_tag="G9P48_RS22665"
                     /old_locus_tag="G9P48_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /name="IS1-like element IS1A family transposase"
     gene            <24198..24506
                     /locus_tag="G9P48_RS22670"
                     /old_locus_tag="G9P48_22695"
                     /pseudo=""
     CDS             <24198..24506
                     /locus_tag="G9P48_RS22670"
                     /old_locus_tag="G9P48_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            26093..27061
                     /locus_tag="G9P48_RS22675"
                     /old_locus_tag="G9P48_22700"
     CDS             26093..27061
                     /locus_tag="G9P48_RS22675"
                     /old_locus_tag="G9P48_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_030005799.1"
                     /translation="MKDQITHLPDNADHSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSNTHEIICANLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTQLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(27201..27587)
                     /gene="stbA"
                     /locus_tag="G9P48_RS22680"
                     /old_locus_tag="G9P48_22710"
     CDS             complement(27201..27587)
                     /gene="stbA"
                     /locus_tag="G9P48_RS22680"
                     /old_locus_tag="G9P48_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_001039464.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRQNRKNVLHNTHDKSSAKGAAESVLHNTQPSEPEVQESKKAESP
                     GIIDKEFFKKIGENFDPKKFNKKF"
                     /name="stbA"
     gene            28168..>29070
                     /locus_tag="G9P48_RS22685"
                     /old_locus_tag="G9P48_22715"
                     /pseudo=""
     CDS             28168..>29070
                     /locus_tag="G9P48_RS22685"
                     /old_locus_tag="G9P48_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(29176..>29886)
                     /locus_tag="G9P48_RS22690"
                     /old_locus_tag="G9P48_22725"
                     /pseudo=""
     CDS             complement(29176..>29886)
                     /locus_tag="G9P48_RS22690"
                     /old_locus_tag="G9P48_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     gene            complement(29940..30644)
                     /locus_tag="G9P48_RS22695"
                     /old_locus_tag="G9P48_22730"
     CDS             complement(29940..30644)
                     /locus_tag="G9P48_RS22695"
                     /old_locus_tag="G9P48_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             complement(2166..2818)
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8181..9037
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11834..12090)
                     /name="mef(B)"
                     /gene="mef(B)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="20.78"
     ARG             complement(29177..29892)
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.58"
                     /coverage="85.61"
     Typing          27814..28071
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.273"
                     /coverage="96.0"
                     /evalue="7.34e-49"
     Typing          complement(20343..20788)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.21"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctccagat agtcatcacc gatgaatcag gctccagtcg aacggaggaa ctgatgacaa
       61 tccagaaatc aggggagacc cggaacgaca tcggattatc ggtgtcagaa tccaagctgt
      121 tgctgaatac ggtccagcag tcggtggtcc agctgcaggc agacgaatat actcaacacc
      181 atatccggtg ccctcactgc cttgctgcgc gcagaatcaa aggcaaacag aaaatacggt
      241 accggacgct gtttggcgtt attccggtat ccgggcttcg ggtgtaccgg tgccgttgtg
      301 aagagagtga tacaaagaca gtcagtttgc tcagcgactg ggccggcgat tattctcacc
      361 cggcactgaa atatattgaa acccgctggg cctcgatgat ctcctatgaa atgacgactc
      421 gtctgctgaa agatattctg ccggtgggcc acagcctgaa tgcctcaacg gtgaggaatc
      481 atttatgcca ggtggcgcag cgtcttgacg ctgaggctga agctcattca ggttttcttt
      541 ccggctgccc ccgcgactgg gggaacctgc ccaggccagg aaaaccgctt gtggtgggta
      601 tagatggtgg ttatgttcgc gatcgggatg acaaaaagcg taattttgaa attattgccg
      661 ggaagtcttt ttccgttggt gcgcctgctg acacccgccg cttcggtttt gttcagaagg
      721 atgactgtca tccggaacgc aggctcatga cccacctttc agcgcaggga atgcaggcca
      781 accagcagat attttttctg tccgacggcg cggataatct cagggacctc cagttcggta
      841 tgtaccctga gtcaacgcat gtgctggact ggtttcatat caccatgagg ctgaaggtgc
      901 tcatgcaata tgcccgggga ctgctggtat cagatccaga ggcggggagt aaagttctgg
      961 cactgctgga aagcattaaa cgatatctct ggcatggcaa tgttgtcgct gcgctggaac
     1021 atattgataa ttgcgttatg tattgcgatg accctgaact cagctatcct agcctgaaat
     1081 ccctgcaaaa acatctggat gaaatgtata cctacatccg gaataataag atgatgatcc
     1141 caaactacgg cgaaatgcgt cggtacggag agccggtatc aaccgcgttt gtggaatcca
     1201 cgatcaatga agtgatcgcc agacgaatgg ccaaaaagca gcagatgcag tggagcagaa
     1261 aaggggctca ttatttgtta cagacccgga ccgccgtttt gaataacgaa ctgcaggata
     1321 aatttgtctg ctggtatccg ggttttcaaa gtgacgggaa gggaccagcg atggcagcat
     1381 agctcacgct gccccaatat tttttatgct ctcggtcgta tggaagctgg accgcctcgg
     1441 tcgtgatctt cgtcatctca ttaatacagt gcacgacctg accgcgaggg ggaccggtct
     1501 gaaagtgctg accggtcacg gggcgactat cgacacaaca acggccgctg gcaagcttgt
     1561 cttcggtatc tttgccgcac tggcggagtt tgagcgcgaa ctgattgctg aaagaacgac
     1621 tgcaggtctg gcatcagcaa gagcccgtgg gcggaacggc ggccgacctt ataaaatgac
     1681 gccagtcaaa ctgcgactgg caatggcatc aatgggacaa tcagaaacaa aggtcagtac
     1741 gctatgccag gaacttggga taacccggca aactttgtac aggcatattt cccctgttgg
     1801 tcaactgcgg gcggacggga tcaagctgct caaccgtgga taatttgaac ggacggaaaa
     1861 atctggggtt ccggcgtagt acccctatca cctccacatt tttgcaacag tgccttataa
     1921 atggcagcct tatgtttact tgccaagatg ggctaccata tgatgtttta tactgagcaa
     1981 tattaatatt atctttaatg gaaaaattaa gcccactaag tgacccactg ctatatggct
     2041 ctattgtgaa gacgcctgag ggtaaaaatt tatacatggt tgtccctatg tctattattg
     2101 caaggttgac aatattattc gttttataaa tttgagcgtt taaaatcaca catcaaagca
     2161 attagtcagg ataaacaaca atacccagtg cttcgagaat cagttcttgc tgccaggctg
     2221 caattttgat acctgatgta tcgactttgc ggggatctaa accgtcgagt tcggcgtggc
     2281 ataaattggc accctgtagg ctaaattgcc cccagacatc ttcggaaaaa acacctcgac
     2341 ttaagtctga ctctttcagt gatgcacccg ctaggttcgt tcctatccag cgattttcaa
     2401 acaactcaca tttttctaaa caaaccctct ccatattggc ataggaaaga ttacatccag
     2461 aaataaatgc tgagcaaaag tacatacgat tactcacttg atgggcaaag tttgttcggg
     2521 aaaagttggc accttttaaa tcacacgcac ggaactctat accgtagcaa ttggcattac
     2581 tgaagtttgc catcgcaagt tggcattgtt ggaaacttgc atcacgaaga tctgcgacat
     2641 caaagtggca gccttcgata tcaccctgtt caatgaactt gcagttgacg aatgtcgtat
     2701 cacgcaagtt agcacgtcga aagtcgctgc gaatgaatgt gcaagcggtg aaggtgagat
     2761 cacttaagtc tttatgtgaa aagttgtggt gccgatatgt atgattgtag gtttccatga
     2821 gtactccttg ttgaaaggag ggctagacta ccattataca tatgccaaga cattaaaata
     2881 aaagcctgtt acatcaacac attacaaccc ctcaatttac ctgttttaag tggtcaaatt
     2941 ggcgcgtaga gcgcctatcg ctcttgcatt ggcacgcggc cctgttgaaa aaagttattc
     3001 gctttagata gggttaggag agccgtgctt cgccggtaaa aaatcggact atccgacgca
     3061 gggcggactc atcggcggag aagcaaactt tgactatatc tccctacact tggaagaggg
     3121 ctagactggc cccatagatc accagacact tcgactctct taaaataaga ggtagtcttg
     3181 gaactatgtt taataccagt cagaacgata agatggttga agtgttatca ggacctgagc
     3241 ggcgtcggcg tcgtacaccg caggaaaaaa ttgccattat tcagcagact atggagcccg
     3301 gtatgaccgt gtctcatgtc gcgcgcctgc atggcatcaa tgcaaaccag atattcaaat
     3361 ggcgcaagca atattaagac ggttcactga cggctgttgc atcgggcgag gaagtggtgc
     3421 cggcctccga actcgctgcc gccaataagc aaattcgcga actccagcgc cttctgggca
     3481 aaaagacgat ggaagccgag atactcaaag aggccgtgga gttcggccgg gcaaaaaaat
     3541 ggattgcgca tgcgccctcg ttgcctggga acgacgaata agcgacgttt gcagaagtct
     3601 tggcgtgtcg cgtgcgcagt tgagcattcg ggttcaccgg ccatctgact gacaggatcg
     3661 cagacggcag ccccgttctg acgataccac cgtattgtcc cggataaaca tggcggtggc
     3721 tgacctgccg acgtatggtt atcgccgtgt gtgggcgctg ctgcgacggg agtcggagcg
     3781 ggacggcctg cctgtggtta atgcaaagcg ggtgtatcgc attatgagaa cgcataatct
     3841 gcttcttgaa cgtaaacccg ctgatccttg ccgtaagcgg gcccataagg ggcgcgtagc
     3901 agtagcggaa agtaaccggc gctggtgctc agatggcttt gagttccgct gtgataatgg
     3961 cgaaaagctg cgggtgacct tcgcgcagga ctgctgcgac agggagatca ttgactgggc
     4021 agcgagcacc ggaggctatg acaaagaaac ggtgcaggat gtcatgctgg gtgcggtgga
     4081 aaaacgcttc ggaaggcagt tgccaacagt gccggcgttc gcccggttgc tgggactgga
     4141 gccttgtacg acagcagtgc gaagcccgga aagcaatggc atagcagaaa gcttcgtgat
     4201 gacgataaaa cgggactaca tcagcatcag gattttaggg gtctgacgct cagtggaacg
     4261 aaaactcacg ttaagcaacg ttttctgcct ctgacgcctc ttttaatggt ctcagatgtc
     4321 ctttggtcac cagttctgcc agcgtgaagg aataatggcc gagcatattg atatgtccgt
     4381 ggcaaagcgg ggagaggcgt gcgatatctt catcattcag tgtttcaccc tgcgcccgga
     4441 gatgatccag ggctgcctgc atataaatag tgttccataa cacgacggcg ttagtgacca
     4501 gccccagtgt gcccagttga tcttcctgac cgtcggtata tcgttttctt atctcacctt
     4561 tttgaccgtg acagatggct ctggcaacgg catggcgact ttctccccga ttaagctggg
     4621 tcagaatgcg ccggcggtaa tcttcatcat caatataatt aagcagatac agcgttttgt
     4681 tgatgcgccc cacttcaatg attgcctgag tcagtccgga aggacgttca cttttcagca
     4741 atgaacggac cagcactgaa acctgtactt tgcccagctt cagggagcca gcggtccgga
     4801 tcatttcgtc ccactgaagg actatttttc ggggatctga ttgccctctg gcaatatcat
     4861 tcagcacgcc atagtcggca tcatggtcca ttcgccagaa aaccgaagca ccggcatcag
     4921 ccaggcgtgg agaaaactgg tatcccagca gccagaaaag gccaaagaca agttcgctgg
     4981 cacctgctgt atcggtcata atttcggttg gattcagccc ggtctcctgt tccagaagac
     5041 cttccagcac aaagatagag tccctcagcg tccccggtat aacgatgcca tgaaagccgg
     5101 aatactgatc ggacacaaag ttgtaccagg tgatccctct gttattacca aagtatttgc
     5161 ggttcggtcc ggcattgatt gttctgactg gcgtaacaaa gcgcattcca tctgcagatg
     5221 ccacttctcc tccaccccat atctgtgcca gtggcagcgt tgcctgaaaa tcaaccagtc
     5281 tggcattagc gctggtgata gtttcagccc gcagatagtt cgcttttgtc cagttcagcc
     5341 ggtgtcgggt cagtgcagga acatttgatc tgatcagtgg ttccagaccg atattgcagg
     5401 cttcagccat cagcacggcg ctgatgctga cgggcagatc atcaactctg gcactggctt
     5461 cactagcatg gaaaaactca tcagcaaatc cggtatgggc gttaatttcg agcagcaact
     5521 ccgttaaatc caccggaggg agtagatcac tgatcatttt gctcagtcgt ttcagactgt
     5581 ccggctcatc aagactggcg aggggagaaa ttgtcaaccg gggcttcggg ccagaaacat
     5641 cgagttcgac agcctcattt tcgcaaagac gtgcagcaac ctgtctgtaa cgactatcaa
     5701 gctgatgacc cagagatttt attgcttcct gcgggtctgt cgggtgcccc aaagaacgat
     5761 aaaccttaat ccggtttgcc tgccagtcag caccctgtag taatcttgca cgaggatctc
     5821 cccaccggtt actgccggta acgtagacat ccctccgcct cagactatcc tgcagtttac
     5881 tgagaaagca gagcgtgtat cccctgcggg tgatatgttt ttccttgtta atcaccagcc
     5941 gtttccatga ccgactgata atttccgttg gtgcgtcgtc aaaaaactgc cgccgtgagc
     6001 tgaactcccg gctgaggtag tcacaggcat tcagagtggt aaccccggca ggtgcggatg
     6061 aaaatttaac ggtattcagc agatggggca ggaaacgacg aacgcgcccg tactgctcca
     6121 ccatttcttc atgaaaatta tcgtctgagg gccgggcaat ttcacggaca agcgtgatga
     6181 tttcagccag cttttgcctt gggatgtagc tgaacacctc agcacgaatc gattcgtccg
     6241 gtgtttcttc tttcagcagg tacgaacatg cgctggcgag cgccaatgca gatttatcca
     6301 gatccttcag cgagcggagc cgttttttct gcccaatctt tctggcgtca cggatgataa
     6361 cggccagcat ggcgtccaga acgtccaatg catcatccag cgccagcgtt tcccatgcaa
     6421 ggacaaaggc aaccagaacc gccatccttt tctgcggtga catcctggca atattgaaca
     6481 ccgaagtcat accagcataa cgtgcgagat ttttcaggcg cacagccggg agtgtactca
     6541 ggttttcagc atgcaggcca aaatcgttca gagttttcca gcgttcaatt gcttcattaa
     6601 acgccggacc actgatggtc acagggccct ttttcagtga ttccagtaaa gacaggcggc
     6661 tgcaatcagt tggccccagc agcatctcca gctgtgaacg ctgttcggct gacggtatca
     6721 gtgccagttt gttccacagg cgcaacgtcg ccttttccct tacctctgaa atcaaccggg
     6781 tcagcgtagt ggctccgggg agaataatac gatgttgcat aagccaccct gtcgccagat
     6841 cgaaaagcag gccaggacgt tcgttgctta tccagctccg ggtatataaa agacgggtaa
     6901 ggcgaaatgt ccagggccag gcaaattcac gatactgata gtgctgacgt atcagcgctg
     6961 catgctcacg gcgggtattt tccctctgac cgtattctgc aagaacggtg atatcacgaa
     7021 tcccgagctg tctggcggta aaatgccgga cgccggaagg aatatgattc atatcggtga
     7081 ggaaggtgcc cagaaaacgg acacatccaa tttgcagggc aatgcccaga cggttgtgat
     7141 cacctctgct ttttccgata aattccttgt ctgcttcatc aaggtgaaaa tatcgtgcca
     7201 gctgaagctc atccggttca ccggtgaatc tgccatagct ttcagtctgc tcagtggtca
     7261 gaaagtcaac gggcatatcg gcctccctgc ctgacgggca tttagtaaca tttttccaac
     7321 cgtacgaaat gttataaatt atcggacatc gtaaaactgt tacattaata tgtctattaa
     7381 atcgtaaatt tgtaataata gacatgagtt gtccgatatt cgatttaagg tacattttta
     7441 tgcgactttt tggttacgct cgggtctcaa ccagtcagca gtctcttgat cttcaggtca
     7501 gagcactcaa agacgcaggt gtgaaagcaa accgtatatt taccgataag gcatccggca
     7561 gttcaacaga ccgggaaggg ctggatttgc tgaggatgaa ggtggaggaa ggtgatgtca
     7621 ttctggttaa gaagctcgac cgtcttggcc gcgacactgc cgatatgatc caactgataa
     7681 aggaatttga cgctcagggc gtggcagtcc ggttcattga tgacgggatc agtaccgacg
     7741 gtgatatggg gcaaatggtg gtcaccatcc tgtcggctgt ggcacaggct gaacgccgga
     7801 ggatcctaga acgcacgaat gagggccgac aggaagcaaa gctgaaagga atcaaatttg
     7861 gccgcaggcg taccgtggac aggaacgtcg tgctgacgct tcatcagaag ggcactggtg
     7921 caacggaaat tgctcatcag ctcagtattg cccgctccac ggtttataaa attcttgaag
     7981 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8041 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8101 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctggtaaa tgcttcaata
     8161 atattgaaaa aggaagagta tgagtattca acattttcgt gtcgccctta ttcccttttt
     8221 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8281 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8341 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8401 atgtggtgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8461 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8521 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgctgccaa
     8581 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8641 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8701 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8761 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8821 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8881 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8941 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9001 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9061 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9121 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9181 agacccctat gattaccctc ttagtaacag ttcgaaagat ccagacctaa gtcaagtacg
     9241 ccaacacaac ttcagagcaa gtatagcata ttcatttaga aagctaaaaa aataaaacta
     9301 acctagggct ttggatattt tcaaggcatc tgataaagac ttaacatcat gggtgcggag
     9361 ataatctgca ccttttttgt atgcatacat ttctgctgca agagttggtg ctaaacgaga
     9421 tttcacatcg gttccagtta ttttacctaa gaatgatttc cgtgacactg caatcattac
     9481 ttgcaaatta aaagcttctt gaatttcagg gaaacgcttc aaaacaagaa tagatgtttc
     9541 tggattagag cctaaaaaga agcccatacc cggatcaaga ataattcgtt cacgctttac
     9601 accagcctca actaaagcag caattctttc tttaaaaaat tccatcatgg aagtaaaaac
     9661 ctcttccgga ttcgtttcaa ctttagtagc tgcaccaatt cgctgaacgg agtgcatcaa
     9721 cacaagtttg caatctgact ttgccaagcc tgaataaatc tcaggataag gaaaaccttg
     9781 aatatcatta ataaaatcaa ccttttgttc tatgcaaaaa ctctgaacct caggtttaaa
     9841 tgtatcaaca gaaatagaaa tgcctttttc ttttaaagcc ttaatgacag gtttgagtct
     9901 tttgatttct tccacaacgc ccacttcagt tgtatcagga ttactggaag cggctcccaa
     9961 atcaatcaca tctgctccat cttcaaccaa atgcagagca tgctcaattg ccttatctgt
    10021 atctaaataa agtcctccat cggaaaaact atcggtggtt atatttacga ttccaaaaat
    10081 cttgctcata tcattttgct ttgattacaa aagtagcatt tatcttaatt gactaattat
    10141 cacttacgca aattgacaca ttttccctat ctttgcagcc taattttttt gttttaattc
    10201 attatgaaaa ctgataggaa ctgtcatttc gagtttttcg cggttttggg tcaaaattcg
    10261 gttttttccc cgaactacga ctgttttcgc accccgaaca actaatccaa tcagaatttt
    10321 ccggcaacgc cgactccaag aagtgtaatt ccagtctatt tctgccgtca acccctgatt
    10381 cgcgttaatg cgacaaagct caaacacata actcccccac gaagccatat aaactccgat
    10441 ttcgaggtgt tatcctgttc cgcataaact tcgtttttgg gtattatacc tacaccaaag
    10501 caagaagatt atgggtccaa acttcatttg tatattttgc aaacaaatat ctgcctgtca
    10561 tatctgtcgg agtgtggtga tactcctttg cattaaaaat gcgctgataa aaggtagttc
    10621 agcaaaaggc gaaacgccaa taaatatatc tataaccggt ttcgcccgtt gattgggtat
    10681 cgccgtgctt ccgatatgtt caatggctac ggcatgagaa ccgaaaaaat ggagcaattt
    10741 tattttctct tcttcaaaat tctttgtcca atccgggtta tattccataa tttcaatttt
    10801 catgatagtt tcgtccttgc ctcaaaaacc atgtttggta ttatactcca tatctctccg
    10861 gtgcaggaat agccttttct gatgcacggc taacagcttc ccattcttcc caggtagcca
    10921 tccgtgcttt taaggtatcc atcgccaaat cgtataccat gctgccaagg ataagcctca
    10981 aaggcggatt attgctggct actaatttca taagggcttc cgctgccaag gaaggatcac
    11041 tatcgaccga atcttcggaa tactgtttcg ccagctcatc gcgcagtgta ccgtatgaat
    11101 ctaatggatt gctgtaactc atcgaagtgt ataaatctgt ccaatatcct cctggttcca
    11161 ccatagtgag tttcactccg aaatgttccg cttcttttgc taaggcttca ctcattcctt
    11221 ccagcgcaaa tttacttgca ctgtaaatac cagacatcgg acccgagata atagcgccaa
    11281 tgcttgtaat ctgtatgata tgtccagaac gctgcgacct caaatagggc atcactgcct
    11341 gacaaaccca aagagctcca aaaaagtttg tgtccattag tttccgagca tcggattcgt
    11401 ttagttcttc aatcataccc atagtcatga tacccgcatt attaacaaca atatcaagcc
    11461 taccgaaatg tttaactgct gtttcaaccg tagaaaaaac agcctcccta tctgtaacat
    11521 cgaggtttaa tggcagtaag ctctcttgat atgtttcttc tagcttcgcc aaattatcga
    11581 tagtccttgc aactgcaaca actttatccc cggctttcaa ggcggcgcat gtaaaagcat
    11641 agccaagccc cttgcttgct ccggtaataa accatactct tttattttcg ctattaggta
    11701 tcatagatat tctcctttag gctattatag aaagattatt ataagcgaaa ttaataccgt
    11761 taaacgccat gagcccgaca attttgagca ctttgttttg tactcccaga agctcactac
    11821 tacaaattat catcaacccg gtcctttttt cagtccactt gcggttttca ttgttacttt
    11881 gtcatagcgt cgtgtcagaa tgcggcagag aacagcgttt actatcaacg caacaccaga
    11941 ccagaaaaac catgtattaa cacctataac ctcaacaacc ggacctgcaa caagtaagcc
    12001 tatcggcata gaaagagtca tggcggtcat caaaagggaa aacaccttgc ccatcatttc
    12061 aggggcaatg ctttcttgaa cataagccat aacaggaaca tggcactgtt gcaaagttag
    12121 cgatgaggca gccttttgtc ttattcaaag gccttacatt tcaaaaactc tgcttaccag
    12181 gcgcatttcg cccaggggat caccataata aaatgctgag gcctggcctt tgcgtagtgc
    12241 acgcatcacc tcaatacctt tgatggtggc gtaagccgtc ttcatggatt taaatcccag
    12301 cgtggcgccg attatccgtt tcagtttgcc atgatcgcat tcaatcacgt tgttccggta
    12361 cttaatctgt cggtgttcaa cgtcagacgg gcaccggcct tcgcgtttga gcagagcaag
    12421 cgcgcgacca taggcgggcg ctttatccgt gttgatgaat cgcgggatct gccacttctt
    12481 cacgttgttg aggattttac ccagaaaccg gtatgcagct ttgctgttac gacgggagga
    12541 gagataaaaa tcgacagtgc ggccccggct gtcgacggcc cggtacagat acgcccagcg
    12601 gccattgacc ttcacgtagg tttcatccat gtgccacggg caaagatcgg aagggttacg
    12661 ccagtaccag cgcagccgtt tttccatttc aggcgcataa cgctgaaccc agcggtaaat
    12721 cgtggagtga tcgacattca ctccgcgttc agccagcatc tcctgcagct cacggtaact
    12781 gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg
    12841 gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca
    12901 ccgactattt gcaacagtgc cgctttgtta accctttttc caaatttgat agcaatagtt
    12961 aatgtttgaa gtaaagtgtt gctcaaaaac aacttcgaag gtatttggaa tactcgggaa
    13021 gaaaacatcc ccctctggct cgatgtcgat cgtcgataag tggagcgtag aggccatggg
    13081 taatgtttct cggtaaattt ctccgccacc agacactata acgtgaccgg tgaattcagc
    13141 tagcctgtcc atggcctctt cgattgactg aaatacaact acattgtcat catttgatgt
    13201 ccaacctgag cgggtaacga ccgcgtattt cctattgggg agtgcgccca tagattcaaa
    13261 cgtcttgcga cccaccagaa gccactgatt gtaggtcaat gctttaaaaa gtagctgctc
    13321 ccctttcgcg gaccagggta tgtctggacc gcaaccaatc acgccgtttt tcgctttcgc
    13381 agccatcaat gatactttca aggttctcat cctgggttaa ctttgtttta gggcgactgc
    13441 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
    13501 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
    13561 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
    13621 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc cactgggttc
    13681 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
    13741 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
    13801 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
    13861 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
    13921 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag cttctgtatg
    13981 gaacgggcat gcggatcagt gagggtttgc aactgcgggt caaggatctg gatttcgatc
    14041 acggcacgat catcgtgcgg gagggcaagg gctccaagga tcgggccttg atgttacccg
    14101 agagcttggc acccagcctg cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg
    14161 accaggccga gggccgcagc ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc
    14221 gcgccgggca ttcctggccg tggttctggg tttttgcgca gcacacgcat tcgaccgatc
    14281 cacggagcgg tgtcgtgcgt cgccatcaca tgtatgacca gacctttcag cgcgccttca
    14341 aacgtgccgt agaaggcact gttgcaaagt tagcgatgag gcagcctttt gtcttattca
    14401 aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg gatcaccata
    14461 ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac ctttgatggt
    14521 ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ccgattatcc gtttcagttt
    14581 gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt caacgtcaga
    14641 cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg gcgctttatc
    14701 cgtgttgatg aatcgcggga tctgccactt cttcacgttg ttgaggattt tacccagaaa
    14761 ccggtatgca gctttgctgt tacgacggga ggagagataa aaatcgacag tgcggccccg
    14821 gctgtcgacg gcccggtaca gatacgccca gcggccattg accttcacgt aggtttcatc
    14881 catgtgccac gggcaaagat cggaagggtt acgccagtac cagcgcagcc gtttttccat
    14941 ttcaggcgca taacgctgaa cccagcggta aatcgtggag tgatcgacat tcactccgcg
    15001 ttcagccagc atctcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
    15061 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
    15121 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccacttcg
    15181 acgactacct gctgccggcc gagaagttcg ccgcactcaa gcgcgagcag gccctgcccc
    15241 tggcgatcaa cccgaacagc gaccagtacc tggaagagcg tttgcagctg ctggacgagc
    15301 agttggccac cgtcacccgc ctggccaagg acaacgagct gcccgatgcc atcctcaccg
    15361 agtcagggct gaaaatcacc ccgctggatg cggcggtgcc ggatcgggcg caggcgctga
    15421 tcgaccaaac cagccagtta ctgccgcgca tcaagatcac cgaactgctg atggacgtgg
    15481 acgactggac gggcttcagc cgccacttca cccacttgaa ggacggggcc gaggccaaag
    15541 acaggacgtt gctgctgtcc gcaatcctcg gtgatgcgat caacctcggg ctgaccaaga
    15601 tggccgagtc gagccccggc ctgacctacg ccaagctgtc ctggctgcaa gcctggcaca
    15661 tccgcgacga aacctattcg gcggccttgg ccgagctggt caaccaccag tatcgccacg
    15721 cctttgccgc ccactggggc gacggcacga cctcatcctc cgatggccag cgcttccgcg
    15781 cgggtggccg gggcgagagc accgggcacg tcaacccgaa gtacggtagc gagccgggac
    15841 ggctgttcta tacccatatc tccgaccagt acgcgccgtt cagcacccgc gtggtgaatg
    15901 tcggcgtccg cgattccacc tatgtgctcg acggcctgct gtaccacgag tccgacctgc
    15961 ggatcgagga gcactacacc gacacggccg gcttcaccga tcacgtcttt gccctgatgc
    16021 acctgctagg cttccgcttc gcgccgcgca tccgcgacct cggcgaaacc aagctgtacg
    16081 tgccgcaggg cgtgcaagcc tacccgacgt tgcgcccgct gatcggcggc accctgaaca
    16141 tcaagcacgt gcgtgcccac tgggacgaca tcctgcgcct ggccagctcg atcaagcagg
    16201 gcaccgtcac cgcctcgctg atgctgcgca agctcggcag ctacccgcgc cagaacggac
    16261 tggccgtggc cctgcgcgag ctgggccgga tcgagcgcac gctgttcatc ctggactggc
    16321 tgcaaagtgt tgaactgcgc cgccgcgtgc atgccggcct gaacaaaggt gaggcgcgca
    16381 actcgctggc cagggcggtg ttcttcaacc gccttgggga aatcagggat cggagcttcg
    16441 agcagcagcg ctaccgggcc agcggcctca acctggtgac ggcggctatc gtgctgtgga
    16501 acacggtgta cctggaacgc gccacccagg ggttggtcga ggccggcaag ccggtggacg
    16561 gcgagctgct gcaattcctg tcgccgctgg gctgggagca catcaaccta accggcgatt
    16621 acgtctggcg gcagagccgc agactggaag acgggaagtt tcggccctta cggatgcccg
    16681 gaaaacctta gcgtacgatt ttttccgaat tctgcgggct cccctattag ccacactcgc
    16741 aagccaagca gagtcggctt aaattctatt gagtgttgta actactagct taaatggcgt
    16801 tcaacttcct cgattttaca gcactactaa ttgtccctcg actacaggcc ataattttct
    16861 gtacctgact ccaggaacta ccgctatcga gcaaccgatt gatggcatcg tatcgctcct
    16921 tattggcctg tcgcccctta taccttccct ctttttgagc tttactaatc ccctgtcttt
    16981 gacgttcccg gcgttgttca tagtcacgac gtgccacagc agccagcatg tcgagcaaca
    17041 tatcgttaat tgcagcaaac atgcggctat caaaatcatg gcttcctgaa gctatccatg
    17101 ttgtcggaac gttgatggcc atcactcgta tatctttctg cctaatcaat tttttcaagc
    17161 tgttccagtc ttcgccagtc agacgtgaga gccgatctac atcctcaatt aagagaatat
    17221 cgttgtgccg gcagtctttg agcaacctga ataattcagg ccggtcaagc ctcgaaccag
    17281 attcattctc aatgtagtaa ttgcaaatgt tcagcccacg ttcctcggca aaactatcaa
    17341 tcgcagccag agcgcgagaa gcatcttgtt cagatgtaga ggcgcgtaaa taagcccgga
    17401 caaagccttt cggtacagtt tgagtcacag tgtagaacca gttcgattta ggtagttaaa
    17461 gcataactat acagtacccg ttatgggtgg tatttaaaag gcatactcaa aaagaaccag
    17521 aaaacagacc tccgaccata aagtgacaat gtcacacaga aatttacaac ataaagatga
    17581 tgtaaagacg gtctcagatt taccacgtaa agccactata aatacacaca aatctttaca
    17641 caataaagat aacataaata caagcttatc tttatttgtg ctttacatgg taaatcatgt
    17701 gtaatataaa gacactgtaa agacaaaata atctttacga acaacacaca atgtaaagat
    17761 aagacagtct ttacataatc tttacgtcat aaagagagga attctgaaat gggtaaaatc
    17821 ttgcttgtcg tatcagataa gggtggtgta ggcaaaagca cctacgtggc taacacaggc
    17881 tcaatgctgg tcaataaagg taagtcggta attatcctca agacagataa aaaccacgat
    17941 ctgttgagct ggaacgaaaa gcggacagat aacggcttac tcactatccc ggttcacgca
    18001 gcttacggaa acgtcagcaa tgaaattaag cgacttagta agctgtgtga agtcctcatt
    18061 gtcgattgcc ccggccacga cagccaggag ttccgtagcg ccctgacagt atcagatatc
    18121 gtggtaacac tggttaagcc gtcctctgat ttcgaaagcg aaactctgac cagtgttaca
    18181 gagaaaatcc gcaccgctca gaaagctaac gcagccctgg aaccgtgggt gctgttcacc
    18241 agaatcaact cagcgaaacc ccgccacaga aaagcggcca ttgatctgga taaattactg
    18301 cgttcggaca acatctggat tcaacctctt aaaacccgga tatccgagct ggatgtgtat
    18361 gaaagcgcct gtaatgaagg ggcaggggta catgacgtta gccgggcatc aagcctttca
    18421 acagcgaaag cacagattga actagtggcg caggaaattg gaattttata gtaatatcaa
    18481 taccatctat aaatctttac attgtaaaga taatataaag atagtatgat atttactatt
    18541 aatgcatacc gtacaagggg aaattgtcat ggctttaaaa ctaaaccgtc cgaacatcga
    18601 tgaatctcag caaccagcaa caaacgcaga aaccgcgcgt tttatctccg gcgctaccag
    18661 agcgcctgta gtgggtaaac caaagttgat gaacttcaga ctttcagccc attttgagga
    18721 catactggaa gctgaagcta tcagaacagg gcagagcaaa acaaccgttc tcaaagcggc
    18781 gctggcgatg tacaacagcc aggatgaaaa cataaagaat cactggttgc ttgagtctgc
    18841 aaaattctaa ccatagaagc atggatgaaa atctatgaga tatttaaccg aattgatctg
    18901 tgacctctac aggttgacag atcaccataa actgaccaca gcaacgttta aaaatcttgc
    18961 tgatattaag ttatttaaac ctacagcaga tgcagacacc atattgcagc ttgaaaatat
    19021 atttagcgaa tactcactga gatatattga caaggatctg gctgaaattc tgagcaactt
    19081 aataactaca gataagaaac actctctgga cttcgatttt aacaaaatac aatctctgac
    19141 tgactcgaaa agtttcggtt gtggatggag caaggtaata aatggtagtt ggttcaaaga
    19201 tctttattct tggggagagg ggatgtatcc tgccgaaaat ctgaaagctg aaaatatttt
    19261 agactggaaa gataatatat ggcacattga gcatgaaggt ttccccccac gcccccctat
    19321 taatgttaaa tattattctt ggtttgaccg atatgtagct tcaaacagtg gtggctcaca
    19381 tcatgcagca atggttgtat atcaatcagt cagagacggg ttagagtata aaagagaagc
    19441 tgttattaaa caactgagta tagacccagg cactgttgaa atacttgata agaattacta
    19501 ttcattcata ttccagatca aaacccttaa taaagaaact agaatttata catctgaata
    19561 cgagtttaca gatgctttaa aagactttgg acaaaacaga cataccatac ttaaacctgt
    19621 taattacgtt tccaacataa aattagtatt tattccaaaa gatgcattaa aaacaaatag
    19681 cgagacattc cgcaactggt ttcattctgc aattagctgc ggaaaaataa tatcactccc
    19741 tgattatctg aaaaatccag cacattatca tacacttcat tactcacatg aagtagactc
    19801 aataacactt ggcgacccaa gcagaaaatt taaactgaaa ggagattctt agcttttata
    19861 taaaaaatca atgttatcat ttgtcagaag atcagcgagg gtaatacatt caagaaccac
    19921 aatatttgtt ccttccaaca aactggcatc aagtatctta tcgaagctga taatcatcat
    19981 tggcttgcgt tgtgttaaat caaagattac caatggaagt attacactgt taattaattc
    20041 aaactcttta aagaaagcat cagtgccata ctcactggct ggaaaaagaa tctcaattaa
    20101 tctcacacca taaaaaacat ggtagttttc tttaaggtta tcctttaaaa tattaacagc
    20161 cttagtatat ttttcgttaa taaactctct cacctgatat tccataaaat tctccgacaa
    20221 gaaccgactg taggtcaccg ggcaaacgtt gcagaatggc gtcagagacg tcattttgcg
    20281 gcgtttgccc tatcctgcat cgcagtgaaa aggcagggac tggcataact gctgagtagc
    20341 atcattgctg gcttatctcg ctactgttct cggtgctttc gtccgcaggt tcgggaacaa
    20401 gtaacttgtg ccaaggcata gttttgttga gcagctccat cagaggctca gccagcagtc
    20461 ccaaaatcca ggttgccgta atcaccggta tagtcagatt ttgtggcgtc cataactggt
    20521 gatcgagatg ataccaagtg atgacacaca aaataccacc cagccagaat acagcccatt
    20581 taaacgcacc aagaatggct aacacaaaaa caaagataaa atgtaaaaca ctcgcgacaa
    20641 gaagacgaag ataaaaccat cctcagccaa gccacttaac aaaagtgctc cctgttttca
    20701 ctgcaaaacg tttcttacgc tccctggcaa tttctttcct gaatttagca cgttcttccc
    20761 cctgagggaa ttgatagatt ttagccatga gctacctcat ttcgatctca aaacgaagtt
    20821 tatgcaatta tcctctgaaa ccgtatggta ttctgatatc taagccctca ctttctttat
    20881 ctattttata agaaaacctt agctttgatg ctcttcttgc gactctctca acgattttaa
    20941 cttcgagatg attaatctct gtatattgat ttattttttc aatatttttt tgcagaaaaa
    21001 ccttattaaa atccttaaaa ttctgataaa ggtaaacttt ttcgccatta tctattctga
    21061 ataattcaag ttcatccttt aatacatcta cttcaatgtc gaaaaatgtc tttttaccac
    21121 tgctgtattg tttcctgata aactgataaa gcatatttga attttgatca gcgagtctta
    21181 cagacgatat caaaacctga gtagtgtaag aatcctttaa catcgtgata tatggctcaa
    21241 tgacatgtga aaaagcaata tcaatatatc cttcactttc aacataatca caaaacgcag
    21301 tcaggttcat aagcctgact gcatcagaag gtaatttacc tctcttcttc gtatgtccag
    21361 agctttgatt gccctgaggt gtgagaagtt gatttcgagg aatgcgaata acactggcct
    21421 gaagttcttc tgccccctct ttcaactgcc ggtaagcacc tgttacatca atatcagcga
    21481 taaatgcata ttcccgagcc gtaatacgaa aggttgcccc ctcagataac tcttcacgtg
    21541 aatcaatctg cgccatagcc ataaacaaaa ttcttctggc agacaaaggc aacgatgaga
    21601 atgtgctgtt aatttcattt ctgtggcgaa tttttgtttt ctttgtaacg ctcagtaagt
    21661 tcatacatta cctgtggttt ttaagttctg atggtgaaat cataaccaca aaggcggaaa
    21721 tatgtaaagt ggtttatcca ccttatgtat ttccaccaaa acacggatca catgaaagaa
    21781 tttttatcat ttatattcaa tatgatagaa atgcgcaggt atccaccttt taacgtcata
    21841 cgttaaatta gagcactata aagaactaaa gaaatgaaga tacaaagata cgaatatctg
    21901 aataaaaaca gaaaaatgcg tacccatcca cctttcagtg cgtacccatc cacctttcag
    21961 tgcgcagcca tccacctttc atgaaaatta cagtgcgtac ccatccacct ttcagtgcgt
    22021 acccatccac ctttcatttt gaggcgacaa aaaacaacac ctatcttaat gaaattattg
    22081 ataaaaaagc tggcacaatg tttgcagaat aagttaaagt aagttaaaga tcttaaagta
    22141 gatctaccga tctacattta agatcttaaa aacaagaagt ttaataagca aaaaaacaca
    22201 aaaaatctgt ggataactct gctcaaaaca aaaaattcac agcgcctgtg gataactttg
    22261 cgtaaacccg gaggacagat cactctgaac caagacaacc acacataaaa acaatatgct
    22321 cattttttaa ccactgtgcg taagtgccta atgtaatccc tgtgctattc cgcttcctcg
    22381 ctcactaact cgctacactc ggtcgttcgg ctgcggcgag cggtgtctcc tttctcaaaa
    22441 ccagaagagt tcagctcata cgatccgttg attgatcctt tttggatctt tcactgagaa
    22501 gccctgaatc gctttctgcc gcttcgttcg atacaaccat ggtgaaacca tgcgaaatcg
    22561 aaaaaatcgc tcagaatgcg atacagcgcg ttttagaggg tatttacaaa acagtcgttg
    22621 ccttgaggaa attttgatca agattgaact cctatccgga tcatggcaac agatcacagc
    22681 aacaaggccg ctaaaaagcg ttgagcggcg ttaaaaaccg ttaaaccgta gctgactaca
    22741 acgaaacggg aaaaaacgct cagaacgcgt tacagagcgt tttaaggcga tgtcggaaag
    22801 agccatatct cacctgttct gatgtttttc tcaacataag cacttttaag agtgctttta
    22861 aagatgctat tatgccaatg agacaagcgg agggcttatg agctatcaaa ttctgacaac
    22921 tacagcggcc agtattactg acctgaaaaa aaatcctatg ggaaccgtag ctgaaggtga
    22981 aggggacgct gttgcgatcc tgaaccgaaa cgaaccggcg ttctattgcg ttccaccaaa
    23041 actttacgcc tactatcggg aactcgctga agatgctgag ttaaacgctg ttgctgatga
    23101 gcgcatgaaa aacccggaaa ttgtgaaggt taacctggat gacctatgaa ctggcttttg
    23161 accgtagagc actgaaggaa tggcagaaac tcggccacac catccgtgaa caattcaaaa
    23221 agaaactggc agaacggctg gaaaatccac gcgtacccgc agcccggtta catggtcatg
    23281 ctgatcgcta taaaatcaaa cttcgtgcat ctggctacag acttgtatac caagtcattg
    23341 atgagaaagt cgttttactc gttattgccg ttggaagaag ggaaagcagc gaagtctatc
    23401 agatcgcaga tttgcgctaa gattaatcat caaatgattg gtgatgctgc caacttactg
    23461 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
    23521 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
    23581 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
    23641 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
    23701 agcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa ctcaggccgc
    23761 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
    23821 aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
    23881 ggctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc
    23941 gtcttatgag cctgctgtca ccctttggtg gtgatatgga tgacggatgg ctggccgctg
    24001 tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt
    24061 gagcggcata acctgaatct gaggcagcac cggcacggct gggacggaag tcgctgtcgt
    24121 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    24181 atcagtaagt tggagtcatt accaaccagt tcaccaccta ctcgctccgc gattgtggca
    24241 gtcttcgaaa tccccggacg atccgccttt atgaaagtct tgctcaattc aaatcttcag
    24301 gcttatgggt tactactcat gcttggttaa atgaccgttt cgtcttaccg gaatcccagc
    24361 agaagaactt ggcagagttg aaacgatctt tccttgatcc tgcactcaag cagataaatg
    24421 agaaaacacc tttacttgct aagtatagta ttgatgattc aggaaaattt ctgttctcaa
    24481 taattgataa gcaaaatccc gtctgacata aatcagcaca catgagcctg tcatttgaca
    24541 aatttttgtc atgaagatgg gcggatttcc acacagcacc ggcgcccggt aaggtgggcg
    24601 gattcccacg gcaccggcgc ccggcaagat gggcggattt ccacacagca ccggcgcccg
    24661 gtaaggtggg cggatttcca cacagcaccg gcgcccggca aggtgggcgg attcccacac
    24721 ggcaccggcg cccggcaaga tgggcggatt tccacacagc accggcgccc ggtaaggtgg
    24781 gcggatttcc acacagcacc ggcgcccggc aaggtgggcg gattcccaca cggcaccggc
    24841 gcgcggcaag gtgggcggat tcccacacgg caccggcgcg cggcaaggtg ggcggatttc
    24901 cacacagcac cggcgcccgg caaggtgggc ggattcccac acggcaccgg cgcgcggcaa
    24961 ggtgggcgga ttcccacacg gcaccggcgc gcggcaaggt gtgggcggat ttccacacag
    25021 caccggcgcc cggtaaggtg ggcggattcc cacacggcac cggcgcccgg taaggtgggc
    25081 ggattcccac acggcaccgg cgcccggtaa ggtgggcgga ttcccacacg gcaccggcgc
    25141 ccggcaagat gggcggattt ccacacagca ccggcgcccg gtaaggtggg cggatttcca
    25201 cacagcaccg gcgcccggca aggtgggcgg attcccacac ggcaccggcg cccggcaaga
    25261 tgggcggatt tccacacagc accggcgccc ggtaaggtgg gcggatttcc acacagcacc
    25321 ggcgcccggc aaggtgggcg gattccccca cacggcaccg gcgcgcggca aggtgggcgg
    25381 attccacggc accggcgcgc ggcaaggtgg gcggatttcc acacagcacc ggcgcccggc
    25441 aaggtgggcg gattcccaca cggcaccggc gcgcggcaag gtgggcggat tcccacacgg
    25501 caccggcgcg cggcaaggtg ggcggatttc cacacggcac cggcgcccgg caaggtgggc
    25561 cgattcccat atcgacatgt atgtagcttg tgttatctgt ggattgtgca gctcagcggg
    25621 tcgctggtcg tatggcggag tgtcccccgt aaccggccgc gtgcggccgc taacgcgcag
    25681 tacggcgccg cgtcccgcag acgggccgcc gttcccgcgc gcagcactgc gcacccccgt
    25741 gggggacgta cggcagctgc gtggcggtga gcgggattag ggctttgcag ctaccgtgtt
    25801 aagttccctg gtgtcgctga aatagttgat gaacatcccc ctaagcgtca gcagcaagct
    25861 gccgccgctt aggccccgga aatcacgccc tttacgtcgt aaacgccgta aagcccgctc
    25921 accggggtta aaagggatta ctttgacttc ttcattgctg ttcgcgagtt aaggctaatc
    25981 agggcagggg gtagggtggc agaatttata gcccggcaac cggctacgcc gggctttcgg
    26041 cgccatttta caacacagac ggctttgttg aataaatcga acttttgctg agttgaagga
    26101 tcagatcacg catcttcccg acaacgcaga ccattccgtg gcaaagcaaa agttcaaaat
    26161 aaccaactgg cccacctaca ataaagccct catcaaccgt ggctccataa ctttctggct
    26221 ggatgatgaa gctattcagg cctggtatga gtcggcaacg ccttcatcac ggggaagacc
    26281 tcagcgctat tctgatctcg ccatcaccac cgttctggtc attaaacgcg tgttcaggtt
    26341 gaccctgcgg gctgcacagg gttttattga ttccattttt acactgatga atgttccgtt
    26401 gcgctgcccg gattacacca gtgtcagcaa gcgcgcaaag tcggttaatg tcagtttcaa
    26461 aacgttcacc cggggtgaaa tcgcgcatct ggtgattgat tccaccgggc tgaaggtctt
    26521 tggtgaaggc gaatggaaag tcaaaaagca tggccaggaa cgccgtcgta tatggcgaaa
    26581 gttgcatctg gcagttgaca gcaacacaca tgaaatcatc tgtgcaaacc tgtcgctgaa
    26641 caatgtgacg gactcagaag ccttcccggg tcttatccgg cagactcaca gaaaaatcag
    26701 ggcagcatcg gcagacggcg cttacgacac ccaactctgt cacgatgaac tgcggcgtaa
    26761 gaaaatcagc gcgcttatcc cgccccgaaa aggcgcgggt tactggcccg gtgaatatgc
    26821 agaccgtaac cgtgctgttg cgaatcagcg gctgaccggg agtaatgcgc ggtggaaatg
    26881 gacaacagat tacaaccgtc gctcgatagc ggaaacggcg atgtaccggg taaaacagct
    26941 gttcggaggt tcactgacac tgcgtgacta cgatggtcag gttgcagagg ctatggccct
    27001 ggtacgagcg ctgaacaaaa tgacgaaagc aggtatgcct gaaagcgtgc gtattgcctg
    27061 aaaacacaac ccgctacggg ggagacttac ccgaaatctg atttattcaa caaagccgcg
    27121 cggggctaac aggtaagatg aaattaaggt tttacgaaca ctgccactgt aattaattac
    27181 cgctactttc ataaatcacc tcagaatttt ttgttgaact tcttagggtc gaaattctca
    27241 ccgattttct tgaagaattc tttatcaata atgccgggac tctctgcttt tttactttcc
    27301 tgcacttcag gctcagacgg ttgtgtattg tgcaatacac tttccgctgc acccttcgcg
    27361 ctactcttat catgcgtatt gtgcaataca ttttttcggt tttgccgcgt tctatacagt
    27421 gccatctcaa gccctttggc tgttaccgaa aagctgtatt cgctgtcgag gatttcagca
    27481 atttcatgcc gcgttagccc tctgtccagc cagttctcga tttcatttag gcgcaatctg
    27541 acagcagccg ataagttgcg gggtttcaga tctgctggtt tattcataac cccctccgtt
    27601 gtatgaaaat gactctacaa cactcgtaag aaggggtcaa tcgagttttg tagtttatct
    27661 gtagttttat tgtagtctat ttgtagtctg tcacgttttt tgtagtttat ttgtagtttt
    27721 attgtagtct atttgtagtc ttacaacact aaggcaaaac gttttttgta gtttatctgt
    27781 agtttatctg tagtttttgg ttgctcagat ggtcatgcaa gcatgaccat caaagaatag
    27841 gacgcttttt agccaaatgc cccttaacct acggagatcg ccctaccaaa aaaagatgat
    27901 tttttatccc ttaacctgct atacacctaa cgcagcgtga taaatcacgc tatcgcactg
    27961 atttaaaaga aaaaaaacaa aatttagcca tgctgcggta ttacccaggt attacaaatc
    28021 aaatctaaat tactgattta aataactttt tatttattac tttggtaata ctatgatttc
    28081 accgtattag acaccctttt ttcgtttaaa aaatgcacaa attgaggact gaatgggctt
    28141 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
    28201 gcagaccgtt ccgtggcaaa gcaaaagttc aaaataacca actggcccac ctacaataaa
    28261 gccctcatca accgtggctc cataactttc tggctggatg atgaagctat tcaggcctgg
    28321 tatgagtcag caacaccttc ttcacgaggc agacctcagc gctattctga ccttgccatc
    28381 acgactgtgc tggtcattaa acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt
    28441 attgattcca ttttttctct gatgaacgtt ccgctacgct gcccggatta cagctgtgtc
    28501 agcaggcggg caaagtcggt taacgtcagt ttcaaaacgt tcacccgggg tgaaatcgcg
    28561 catctggtga ttgattccac cgggctgaag gtctttggtg aaggcgagtg gaaagtcaaa
    28621 aagcatggcc aggaacgccg tcgtatatgg cgaaagttgc atctggcagt tgacagcaac
    28681 acacatgaaa tcatctgtgc agacctgtcg ctgaacaatg tgacggactc agaagccttc
    28741 ccgggtctta tccggcagac tcacagaaaa atcagggcag catcggcaga cggcgcttac
    28801 gacacccggc tctgtcacga tgaactgcgg cgtaagaaaa tcagcgcgct tatccctccc
    28861 cgaaaaggtg cgggttactg gcccggtgaa tatgcagacc gtaaccgtgc agtggctaat
    28921 cagcgaatga ccgggagtaa tgcgcggtgg aaatggacaa cagattacaa ccgtcgctcg
    28981 atagcggaaa cggcgatgta ccgggtaaaa cagctgttcg ggggttcact gacgctgcgt
    29041 gactacgatg gtcaggttgc ggaggctatg gggtcgtttg cgggagaggg cgaaatccta
    29101 cgctaaggct ttggccaacg atattctccg gtaagattga tgtgttccca ggggatagga
    29161 gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc
    29221 tagatcgcgt tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc
    29281 gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc
    29341 catctgtgca atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa
    29401 catattggcg gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca
    29461 gccgcgactg gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc
    29521 cgaggcattg ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca
    29581 acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg
    29641 gagaagggca gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc
    29701 tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc
    29761 cccggcatat tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct
    29821 cccattgcgc cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg
    29881 tttcaatggc actgttgcaa agttagcgat gaggcagcct tttgtcttat tcaaaggcct
    29941 tacatttcaa aaactctgct taccaggcgc atttcgccca ggggatcacc ataataaaat
    30001 gctgaggcct ggcctttgcg tagtgcacgc atcacctcaa tacctttgat ggtggcgtaa
    30061 gccgtcttca tggatttaaa tcccagcgtg gcgccgatta tccgtttcag tttgccatga
    30121 tcgcattcaa tcacgttgtt ccggtactta atctgtcggt gttcaacgtc agacgggcac
    30181 cggccttcgc gtttgagcag agcaagcgcg cgaccatagg cgggcgcttt atccgtgttg
    30241 atgaatcgcg ggatctgcca cttcttcacg ttgttgagga ttttacccag aaaccggtat
    30301 gcagctttgc tgttacgacg ggaggagaga taaaaatcga cagtgcggcc ccggctgtcg
    30361 acggcccggt acagatacgc ccagcggcca ttgaccttca cgtaggtttc atccatgtgc
    30421 cacgggcaaa gatcggaagg gttacgccag taccagcgca gccgtttttc catttcaggc
    30481 gcataacgct gaacccagcg gtaaatcgtg gagtgatcga cattcactcc gcgttcagcc
    30541 agcatctcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac
    30601 agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccatcagc
    30661 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgcc
//
