LOCUS       NZ_CP049975             1780 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain ARL09/232 plasmid pCO_Eco4457-8, complete
            sequence.
ACCESSION   NZ_CP049975
VERSION     NZ_CP049975.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14351533
            Assembly: GCF_013376575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Olivera,C.D. and Rakonjac,J.
  TITLE     Complete genome assembly of New Delhi metallo-beta-lactamase-1
            (NDM-1)- producing Escherichia coli human isolate from New Zealand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Olivera,C.D. and Rakonjac,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) School of Fundamental Science, Massey
            University, Tennent Drive, Palmerston North 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2020
            Assembly Method                   :: Unicycler v. MAR-2020
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 02:37:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,159
            Genes (coding)                    :: 4,937
            CDSs (with protein)               :: 4,937
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 77 of 222
            Pseudo Genes (incomplete)         :: 134 of 222
            Pseudo Genes (internal stop)      :: 53 of 222
            Pseudo Genes (multiple problems)  :: 41 of 222
            Pseudo Genes (short protein)      :: 2 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049975.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ARL09/232"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /culture_collection="NZRM:4457"
                     /db_xref="taxon:562"
                     /plasmid="pCO_Eco4457-8"
                     /geo_loc_name="New Zealand"
                     /collection_date="2009"
                     /collected_by="Institute of Environmental Science and
                     Research (ESR)"
     gene            complement(join(1538..1780,1..747))
                     /locus_tag="G9448_RS26150"
                     /old_locus_tag="G9448_26230"
     CDS             complement(join(1538..1780,1..747))
                     /locus_tag="G9448_RS26150"
                     /old_locus_tag="G9448_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_032219167.1"
                     /translation="MQNPKLQNLTDYSPADAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNAGFQRLKDRKEFSPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMLSGRDYIKHERWVDLWRGCLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          1621..1780
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.516"
                     /coverage="83.0"
                     /evalue="8.17e-72"
ORIGIN
        1 gtcagccggc tttgtggaat atttcagggt ttcggccact gcgccgcgca ccagttcggc
       61 ggtcgcgcag gccagcgatt cgccgtcctt cggcttccgg ggttttactg cccggacatc
      121 gacattcgga tcgtaatcca cccgaagaca cccacgccac agatcaaccc agcgctcatg
      181 cttgatgtaa tcacgcccag acaacatcga tggcggcacc atcatcaggg tgtgaaagtg
      241 cgggtgtgcg ctgccgtcgc tcccgcgggt gacttccgtg gtacgtatcc agccctgcac
      301 tggactgaac tccttgcgat ccttcagtcg ctggaaacct gcgttcatcc ggttcagcgt
      361 ctcgcccagc tccccgatcg cacagttacg cacagtcagg gtcaggaaca tccagcgggc
      421 atcgggataa tccgccacga tacgcggcag ggactggtag aaacgggctt gccacatcag
      481 ggagcgcctc cactggcaga caggacagtg ccggacacgg cagaaatgcg cctcacgcag
      541 cctcaggcgc gtttctccgg tttctttgag ggtagaccac ccgaaccgca gcaaaccgcc
      601 acaggaggcc atacgcgccc cgtagcgctc atattcagcc gcaagcaggt aaatccctcc
      661 aacgtcgtca gaaacgcttc tgtgagcgtc ccagggcgca tctgcggggg aataatccgt
      721 gaggttctgg agtttaggat tttgcatgca caccgcccca tactgggcga tggctcattt
      781 gacgtgttat cattgccgta tcacctgtgt tgtctgtcgc aaaatagaat tcctacaggt
      841 ggtcacgccc ccgaacgatt gccctcgttg cgggggtcgc ttttaacgcg accactccaa
      901 acagagtact tccaaaccca tttccaagca aatcacctct caggccttgc cgcacaaggg
      961 ctgaggccga attccaatcc cagagttatc ccttaagtat aaatacaagg aacgcgcgcg
     1021 aattttccac ctaatttccc cgtacatccg ccccgccaga aaacccaaaa acgcttatca
     1081 ggtggttgcg ctcactgcgt gatcacaacc acctgccggc agtgttttat gggtgcagtc
     1141 gtcaatcact aaatcggaac ccactggcaa gccagcggga ccccacgatt tagcgtttga
     1201 cgattccagt aacggcgaac gctgacagat ttcaatctgg cggcgtgagg tgttggcagg
     1261 gtgtcggggc gcagccctga cggatcgttt ttggtaccgg catcgtgatg gcggtactcc
     1321 aaaaacacat cttgtcccgt taaaaaaatc cgtattacgg atcgcaaaac cctaagttcg
     1381 gattccgaac tcagtaagcg attggcgtca ctttgcggcg gtagccctat cctgcatcgg
     1441 agtaaaatca atcggacaag atgtgacttt ggtacaccgc caccaaggtg acggatgccc
     1501 aaagtcctca tggtcagggc gcagccccga caccccgtta ctctgattcg gttttatctt
     1561 tcgtcggtga gcgacggtac tttctgacct cagttttcca ctcaaacgcg atgcgggaac
     1621 cgtcatcgtc gccctcggaa atatcatccc cgatcaccat gtctgcgtcg gtttcctgat
     1681 cgagcttcag tacgtctttg agtgcgccgc cggtcgccac aaatcggcgc ttgtgcgtct
     1741 ggcgggtcag ttcgaggaac cattcaggat cggccaccat
//
