LOCUS       NZ_CP049973             2883 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain ARL09/232 plasmid pCO_Eco4457-6, complete
            sequence.
ACCESSION   NZ_CP049973
VERSION     NZ_CP049973.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14351533
            Assembly: GCF_013376575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Olivera,C.D. and Rakonjac,J.
  TITLE     Complete genome assembly of New Delhi metallo-beta-lactamase-1
            (NDM-1)- producing Escherichia coli human isolate from New Zealand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Olivera,C.D. and Rakonjac,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) School of Fundamental Science, Massey
            University, Tennent Drive, Palmerston North 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2020
            Assembly Method                   :: Unicycler v. MAR-2020
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 02:37:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,159
            Genes (coding)                    :: 4,937
            CDSs (with protein)               :: 4,937
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 77 of 222
            Pseudo Genes (incomplete)         :: 134 of 222
            Pseudo Genes (internal stop)      :: 53 of 222
            Pseudo Genes (multiple problems)  :: 41 of 222
            Pseudo Genes (short protein)      :: 2 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049973.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ARL09/232"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /culture_collection="NZRM:4457"
                     /db_xref="taxon:562"
                     /plasmid="pCO_Eco4457-6"
                     /geo_loc_name="New Zealand"
                     /collection_date="2009"
                     /collected_by="Institute of Environmental Science and
                     Research (ESR)"
     gene            join(2651..2883,1..4)
                     /locus_tag="G9448_RS26120"
                     /old_locus_tag="G9448_26200"
     CDS             join(2651..2883,1..4)
                     /locus_tag="G9448_RS26120"
                     /old_locus_tag="G9448_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            341..976
                     /locus_tag="G9448_RS26125"
                     /old_locus_tag="G9448_26205"
     CDS             341..976
                     /locus_tag="G9448_RS26125"
                     /old_locus_tag="G9448_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351244.1"
                     /translation="MNDIFSLIESSRKIKKESIASTVRMPESLHTFIETLACDLELSKQ
                     EIMLKLLEQGARSAQEALAEIEKKELVELGAVEQLEPQTAAGFHILNTNKAHSDEDSEW
                     MLGKGIAAAFYDPWKWNINRIKPNEVVFLYENGKGIVAYGRGTGEVKIRDYHGDKDECH
                     YQELEGFKILENPLSAAQIKKILERNVVFLRTMSAMPDGQKVLNLIEG"
                     /name="hypothetical protein"
     gene            complement(1080..1388)
                     /locus_tag="G9448_RS26130"
                     /old_locus_tag="G9448_26210"
     CDS             complement(1080..1388)
                     /locus_tag="G9448_RS26130"
                     /old_locus_tag="G9448_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351245.1"
                     /translation="MTDKLKGTASVLNQTKTYEELVQKHSPEVANGLLANAINNALPNA
                     GITSNDVAGFSKVTTALRTGEVDLAKTAEEANADAEAVSANILAGLTAKQKSTDEIK"
                     /name="hypothetical protein"
     gene            complement(1699..1812)
                     /locus_tag="G9448_RS26135"
                     /old_locus_tag="G9448_26215"
     CDS             complement(1699..1812)
                     /locus_tag="G9448_RS26135"
                     /old_locus_tag="G9448_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058651821.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPLAVATGN"
                     /name="hypothetical protein"
     Typing          complement(1704..2193)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.112"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ctgattgcgg attttggcgg tttagtggtg ctgacgctgc gaaatcacct gcgattcacc
       61 cgcgtgcata gccatgcagt cgcgcagaaa tcatcagggg cgtttttccg ggatgcgggt
      121 gaggaaatgt gccggaaacg gctctgatgc gccagaattg cgctgagggc ctgtcagggg
      181 gtgctgataa cggatgttat ggtaaataca catcgtaatg actattcgtg catacgccat
      241 catctatcac ctggagttag aatctatttt gattcaatta ttgcatcgtt ttttgattcg
      301 ctatatgctt ctataaccaa cccaatttaa ggttcataaa atgaatgaca ttttttcttt
      361 aatcgaaagc tccagaaaaa ttaaaaaaga atcgatagct tccacagtgc gaatgcccga
      421 gtcgttgcat acgtttatcg aaacgctggc ctgcgatctc gaactttcaa agcaagagat
      481 catgcttaaa ctccttgaac aaggagccag gagtgctcaa gaggcgttag ctgagattga
      541 aaagaaggag ttggttgagt taggcgctgt agaacaactc gagccgcaga cagccgctgg
      601 cttccatatt ttgaatacaa acaaagctca tagcgatgaa gactctgaat ggatgcttgg
      661 caaaggaatc gctgctgctt tttatgatcc atggaaatgg aacatcaatc gaatcaagcc
      721 caatgaagta gtgtttctgt atgaaaacgg taaaggcata gttgcctatg gtaggggtac
      781 tggtgaggtt aaaattcgcg attatcatgg cgataaagac gaatgccact accaagagct
      841 ggaagggttt aaaattcttg aaaatccgct atcggctgct caaattaaga agatattgga
      901 gcggaatgtt gtgtttttga gaacaatgtc ggctatgcca gatggtcaaa aggtgctgaa
      961 ccttatcgaa ggttaagccg agcacttgat ggggttgagt gcggttaaaa tttttttata
     1021 agtaattaca tacgaaacct tggcccgtct agttcgggct tttttgtacc tgcgggaaat
     1081 tattttattt catctgtact tttttgtttt gctgtcaggc cagcaagaat atttgctgaa
     1141 acggcttccg catcggcatt ggcttcttcg gctgtttttg ctagatcaac ttctcctgta
     1201 cgcaaggccg tcgtcacttt gctgaatcca gcgacgtcat tcgaagttat tcctgcgtta
     1261 ggcaaagcat tattgatagc atttgccagc aagccgttag ctacttcagg tgaatgcttc
     1321 tgaaccaatt cctcataagt tttggtttga ttaagaacgc tagctgttcc tttgagtttg
     1381 tctgtcatgt aaacctctag tctgtgatga gtcaggagta gaaaatttta tgtacaagac
     1441 tcgcgttagg cgggcactca tactgaagcc aaatcactgc taaacagccg gatcttcttg
     1501 tgatcctttt actctcgtcg taatctcatt ccctgtaaac aaaaaaacca ccgctaccaa
     1561 cggtggttcg tttgccggaa tcaagagcta ccaactcttt cttccgaagg taactggctt
     1621 ggaggagcgc agtcaccaaa actgtccttt cagtgtagct gcatcgcacc aggaacttca
     1681 agactctcct cctctaaatc agttaccagt ggctaccgcc aatggcgatt tgtcgtgtct
     1741 ttccgggttg gactcaagtt gatagttacc ggaacgggcg tggtagtcgg actgaacggg
     1801 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga gtgtcaggcg
     1861 tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     1921 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg
     1981 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     2041 atggaaaaac ggctttgccg tggccttctt gcttccctgt taagcttctt cctggaagat
     2101 gccaggaaat ttccgccccg ccagtaagcc ggtaccgctc gccgcagccg aacgaccgag
     2161 cggagcgagt cagtgagcga ggaagcggaa tatctccggg cttatctttg gcaccgtacg
     2221 cgccatagct gcatttttta atacgatgca ggatagggca aacgccgcaa aatgacgtca
     2281 tccgacatca ttccgccgcg ttcgcccagt gacctacagt cggctctggg gtgtcggggc
     2341 tgtgccctga ccaggcgttt tggagtatcg ccaccgtggt ggcggtgcta caaaacatat
     2401 cctgtccctt ccttggcatc tctgaagttt agccagtata cactccgcta gcgctacgtg
     2461 actggttcag ggctgcgccc cgaaacccgc taaatccact gacgcgcctg cggcttgtcc
     2521 aacgcctcac ggcaccggat taaaatccgc tcccgttcac cgtttctgac tgcctttcac
     2581 cgtcgcttac gcttcatcgt tcctactgtg tctggcagac tgcagggact attctcatac
     2641 ggagataaac atgacgagac tgacaaacct gacccccgct gagaaaaagt ttatcgacga
     2701 cgctatagcc gctgccgaac gtgcggccgg gaagaagctc aatcagccta accggcacat
     2761 cgtcctgaac agagcgcgtg cgcagattga gtcgcagcgt tacgccgacc gccagcgtgc
     2821 attacgtgag gatgagcggc agcagtccga ttttgcctgg tcacgtccgc gcgcgccgcg
     2881 ccg
//
