LOCUS       NZ_CP049972             3359 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain ARL09/232 plasmid pCO_Eco4457-5, complete
            sequence.
ACCESSION   NZ_CP049972
VERSION     NZ_CP049972.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14351533
            Assembly: GCF_013376575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Olivera,C.D. and Rakonjac,J.
  TITLE     Complete genome assembly of New Delhi metallo-beta-lactamase-1
            (NDM-1)- producing Escherichia coli human isolate from New Zealand
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Olivera,C.D. and Rakonjac,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) School of Fundamental Science, Massey
            University, Tennent Drive, Palmerston North 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2020
            Assembly Method                   :: Unicycler v. MAR-2020
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 02:37:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,159
            Genes (coding)                    :: 4,937
            CDSs (with protein)               :: 4,937
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 77 of 222
            Pseudo Genes (incomplete)         :: 134 of 222
            Pseudo Genes (internal stop)      :: 53 of 222
            Pseudo Genes (multiple problems)  :: 41 of 222
            Pseudo Genes (short protein)      :: 2 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ARL09/232"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /culture_collection="NZRM:4457"
                     /db_xref="taxon:562"
                     /plasmid="pCO_Eco4457-5"
                     /geo_loc_name="New Zealand"
                     /collection_date="2009"
                     /collected_by="Institute of Environmental Science and
                     Research (ESR)"
     gene            join(3354..3359,1..525)
                     /locus_tag="G9448_RS26105"
                     /old_locus_tag="G9448_26185"
     CDS             join(3354..3359,1..525)
                     /locus_tag="G9448_RS26105"
                     /old_locus_tag="G9448_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(1863..2249)
                     /locus_tag="G9448_RS26110"
                     /old_locus_tag="G9448_26190"
     CDS             complement(1863..2249)
                     /locus_tag="G9448_RS26110"
                     /old_locus_tag="G9448_26190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            2416..3351
                     /locus_tag="G9448_RS26115"
                     /old_locus_tag="G9448_26195"
     CDS             2416..3351
                     /locus_tag="G9448_RS26115"
                     /old_locus_tag="G9448_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     Typing          2417..3348
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcaccgaag aaaaacttct cacactcctg ctgtcggcgg agcatctgaa agaggccgca
       61 aaagagcagc agaaagccgt ggcggattcg ctggcggtgg caagggaaac cctgaaaggc
      121 taccgggaaa tatcggacag tgtggcacag caggtaaggg atgaagtccg gcaggaagtg
      181 cagaaaatgg atgttgcggg gctgattggc gagcgtatca gcgctgattt tcagcgtctg
      241 gaaaaatccg tcgggaacat gaaaatcaat gcggacgcgc tggacaagga aatttccatt
      301 atccgcagta acatcatcgc ggagtacaac acgctgcggg gttacagctg gaagtggttt
      361 gctggggttg cggttgcctt tgtcgtactg atatttgcca caagctggtt tgtgaaaacg
      421 gaactacagg acaattacaa cgcgtctgct gccgtctatc agaaagtggc agaccttgaa
      481 aagctgctga caacggaaca gaaaccggaa aagaaaaaga actgacgcgc tgcgcttgtc
      541 caacgcctca cgttcgcaca ataaattgcg cacccgttca ccgttttcag ccgagtttgg
      601 ggcgtctttt gagacttccg gcctgaattc aggccggaag ttctcgccag tcagtcaggt
      661 gcatggttat gcatggggct gaaaatcacg gtatgcgatt ctgagcggtt ttcggtgaga
      721 agccgggggt ttttatggct caggacttaa aaccgccctg agcgcgtttc agcggtgcgc
      781 gtaacggatg ttatggtaaa tccggtggct ttgacgtgcc tgccgttgtg taccgtcgtg
      841 agtgaaaata attatattgt tcgaagaaca gcgctttttg gccggagaaa agcagttctg
      901 caaacgatgg tatatcgata tgagttcttt tggcattaat ccaggcaaga cgccgggcaa
      961 taagcgtaat attccggatg ttacttattt ccatttctgt caacagcatc cccccccacg
     1021 ccggagaata aaaaagccgc ctgttccatc ggcaagccct acatcaaggg aaactacgtc
     1081 ttacgattcg ttttgaaccg ggacgctttg ttgctgcgaa tcactaacca gataatccac
     1141 gttttgtgag tgggtttagg ttaaggcttc aatagaacaa taatagcgtt aagtgattag
     1201 ggcaacctgt gattgctaaa atgatgaagt cttcacatca tttcggtgaa ggcgatatgt
     1261 gttacaccac aacccggatt tgagagtcgc aaacccatat tagggcaaag aaagcctaat
     1321 gcccccgtgg tgggtacacc agaaaacggg gagcgaagcg accagcatca ggccgttcgt
     1381 atgctgcttc gattttgtgg gtgttgcgcc agaccttggc aatcacgctt caccgcaacc
     1441 gctgccttca ctctcacttt atcaggtacg ttattggtta acgtgagacc gggtagagcc
     1501 agcttctttc ccgagctggt caggttccca ccgcttgcct gtttatcctg ccggagtccg
     1561 gggcgtagac ctgagagtca aggtgcagat atgggtttaa tgtcgttgaa tggtattaag
     1621 ctggtagtga cattcctagc cagccctgct aggatttgat tgtgacgtct gatcctggca
     1681 ggtatctgca agaattagag tactcaagcc ccgtgattct gtcacggggc ttttgtacat
     1741 ctggagcaag aaaattcatg ctcctatccg aaaatcccta ccgctcgccg cagccgaacg
     1801 accgagcgac gcgagtcagt gagcgaggaa gcggcatttc tccggccttg cagaatgcac
     1861 gttcacgccg atgtttccag atcgtagcgt acccgtttca gcgcagtcag taatttttcg
     1921 tggtcaaaat tcaactcacc cgtttttgta tcaaccagtc ccgcgtcaat catcagacca
     1981 gccgccagaa acagtctgtg attgcgttcc ctgcggtttt tctgccctga aatccgcgcc
     2041 tgaaccgcct ttttctgtgc ggcatcccgt tcctgcaacg cgtttttttc agcggttgcc
     2101 agaacgtgga gcagtcgtct ttcagtctca ctgagcgaag cttcatcatc cagcagatcg
     2161 ataatctgcc gttgccgtgc cgttttttct cccagctttc tgatgaaggc tatgcgcttt
     2221 ttaagccatt cgacgttact gatccgcatc gtttaacttc cccatcccga tatttccgac
     2281 ggggaaagta tataacttgc ggtcagaagt cgcatgactt atctttactc ataattcttt
     2341 aaattagagt aaagcgcact tatatgtttc tccctgcggg cagaaacagt gcgccctgcc
     2401 gggggctgac acaacatggc aacgtatcat ctcagcgtga aatttggcgg caagggtaaa
     2461 gcccttgctc atgccagtta cattacgcgg gaagataagt tcagccagcg ccaggatctc
     2521 gagcacaccg aacacggcaa tatgccggaa tgggcgagag atgaacccgc ccatttctgg
     2581 caggctgcgg atgcgttcga acgtgccaac ggttcaacgt acagggaaat tgagatcgct
     2641 ctaccccgcg agctgaatga agcgcagcgg ctggcgcttg tccgggattt tgtgaagcag
     2701 gaaacgggcg acaaacatgc atggacgttt gcgatccaca atccgaaagc cagcattgac
     2761 ggcggcgaac agccgcacgc ccatatcatg atgagccagc gcgtcaatga cggtattgag
     2821 cgcagtcccg aacagtattt caggcgctat aacgccaaat ttccggcgcg tggcggcgcg
     2881 cgcaaggact cagggcataa tttaacccct acagaacaaa aacaggcact gaaagccctg
     2941 cgggagcgct gggagcgtac gcacaacgcg catatgaaac gatatggctt tgagcagggt
     3001 tttattgata gccggtcact gaaaacacag ggtatcgacc ggaaaccgga acagcatctc
     3061 ggttttgaag cagccggacg actggacgac agccagaggc aggatattaa agacagccgg
     3121 acgttttcag tcgtggatgt gcctgaacag gcgaaacata tcgaccacag cgaccgtagc
     3181 tggatgaatg ccagcggtat cgaggtaaac cggaaggaac tggtcagatc tctgacgcat
     3241 gatatctcgc agcagtacgg gcgggagatg gctgcacggg tcacaaaaca gggaaatgtt
     3301 gaaatagagc gcgtgaaatc cctgtcacgc ggattttcca taagcaggta actatggcg
//
