LOCUS       NZ_CP049855             4072 bp    DNA     circular CON 08-SEP-2023
DEFINITION  Escherichia coli strain UPEC-774U/190308 plasmid pEcoUTI1b, complete
            sequence.
ACCESSION   NZ_CP049855
VERSION     NZ_CP049855.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14277526
            Assembly: GCF_019443685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Velez-Sanchez,D., Ochoa,S.A., Cruz-Cordova,A., Lopez-Martinez,B.,
            Parra-Ortega,I., Escobedo-Munoz,A.S., Bustos,P., Cevallos,M.A. and
            Xicohtencatl-Cortes,J.
  TITLE     Comparative Genomics of Uropathogenic Escherichia coli Strains
            Isolated from Adult Women with Recurrent Urinary Tract Infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Velez-Sanchez,D., Ochoa,S.A., Cruz-Cordova,A., Lopez-Martinez,B.,
            Parra-Ortega,I., Escobedo-Munoz,A.S., Bustos,P., Cevallos,M.A. and
            Xicohtencatl-Cortes,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) Centro de Ciencias Genomicas, Universidad
            Nacional Autonoma de Mexico, Circuito Universidad, Cuernavaca 66210,
            Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-FEB-2020
            Assembly Method                   :: Unicycler v. v0.4.1
            Assembly Name                     :: UTI-1_GENOME
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 189.523x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/08/2023 18:02:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,020
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,672
            CDSs (with protein)               :: 4,672
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 86 of 229
            Pseudo Genes (incomplete)         :: 154 of 229
            Pseudo Genes (internal stop)      :: 39 of 229
            Pseudo Genes (multiple problems)  :: 47 of 229
            Pseudo Genes (short protein)      :: 4 of 229
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049855.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="UPEC-774U/190308"
                     /isolation_source="bladder epithelial biopsy from
                     50-year-old female patient with recurrent urinary tract
                     infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEcoUTI1b"
                     /geo_loc_name="Mexico: Mexico City"
                     /collection_date="2019-03-08"
                     /collected_by="Hospital Militar de Especialidades de la
                     mujer y neonatologia"
     gene            408..1109
                     /locus_tag="G9W34_RS24820"
                     /old_locus_tag="G9W34_24750"
     CDS             408..1109
                     /locus_tag="G9W34_RS24820"
                     /old_locus_tag="G9W34_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1367..3514)
                     /gene="mobQ"
                     /locus_tag="G9W34_RS24825"
                     /old_locus_tag="G9W34_24755"
     CDS             complement(1367..3514)
                     /gene="mobQ"
                     /locus_tag="G9W34_RS24825"
                     /old_locus_tag="G9W34_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3693..3947
                     /gene="mobC"
                     /locus_tag="G9W34_RS24830"
                     /old_locus_tag="G9W34_24760"
     CDS             3693..3947
                     /gene="mobC"
                     /locus_tag="G9W34_RS24830"
                     /old_locus_tag="G9W34_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(3157..3515)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3516..3692
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          409..1109
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1371..3601)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaacccgcat gaatactggg tttccacagg gttggagggg gtgtattcga aacttgcaat
       61 tctgctttat ttcaacgcat tgtttttatt ggttttttgt tttcagcacc ccaagttatc
      121 cacaggctcg ccgttaagtt tttaagatct tatttttaga tctttttttt aaaacctttt
      181 taggtcttgt aacaccatga ttttaaagat gttctggcaa gataagtgga gcttttgggg
      241 gtgaaaggtg gagcttttgg gggtgaaagg tggagctttt gggggtgaaa ggtggagctt
      301 ttgggggtgt aatccacaga aaaagggggt tgcaaaaaaa aaacttccac ccatatgata
      361 agtggcgtac agtaaaaaaa cgccaccaat agcaacgagc aagcgaaatg tcagatctga
      421 tagcttacaa atcaaacgcc cttatcgagg caagctacaa gctcactttg caggaacagc
      481 gcttcctgct ggtctgtatc agccgcctga aatccggaga agattcccct tcgccggatg
      541 aacaaaaaac catgaccatc acggctgcag agtttcatga tgcctttcct gacatgggac
      601 ggcagcacgc tgaagctagg ctcaaggaag cgatagatcg cctctgggac aggtcagtaa
      661 tcatcaagaa cgaggaaaaa cgagaagagt tccgctgggt gcagtaccgg gcgcagtatt
      721 tcaaaggtga ggcgaaagta gatatcacct tctccgatgc catcatgccg tatctgacac
      781 agctcaaggg gcagtttacg cgggtagtcg tgaagaacgt ctccagcctt tccagcacct
      841 acagcatccg catttacgag ctgctccagc agttccgcag caccggtgac agaacgatag
      901 ctatcgatga tttccgaacc atgcttgccg ttgaagataa atatcaacaa ttcaaagact
      961 tgaataagat gataatcaag ccagccatcg ccgaactgaa cgaaaaaagc gatctggttg
     1021 taactgttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt cgcttcaagg
     1081 aagacaaaca gatcaagatg acgatctaaa atcgatttta aggcacctaa aacgctccct
     1141 gctgcgtttt ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa ttgcgtcaaa
     1201 aacgctctga tgcagcgaaa aaagaccccc caatttttgg gggcggaaaa acaaaactat
     1261 gcttgcgtta tgtgagtgtg cttcttgcat tcatccctgt cgatgaacat cgaagaacat
     1321 cgacgaacat aacacgatgt taaacaaaac aaaatattgt tcacattcac tgtgaccaac
     1381 cgcgatcatg tctcctctcg gtggtgtgtg ttcgtgcatc ttcctcgcgc tgccattcct
     1441 cccaggtacg attaaaaccc agcgagcgga acagatgatc cggctgtttt tctggtgtgt
     1501 tgacggcagc aaaaagcggt gcctcacggg ttttcagatg aaactgcatc agttctggtg
     1561 caggtgatat gtggctgtct gcatcgtgat ttacggcaac accataccct gcataccgta
     1621 accccttatc ggtcagcgca ctgatgacca gatgcctgtt atcggctgag ggaacggcaa
     1681 acgcgggata cagcgtacca ttaagccgga taacaccgcc cagagagtca tccacgccca
     1741 tgaccagcaa aagctgaagc tggcgggttt cccggtcgac agaactcatg accggcatca
     1801 gcgtcggtgg cagacgtccc tgcataaaat ccaccttcct tgccgctggt gatggcatgg
     1861 ctggctggcg gtcttctcca agacagaaag actccggcca tgtcttcggc tggcggagat
     1921 tgcgggtatg ttgcggccat gtctggcaaa cctgcgtcag cagttgctca aaggttgcag
     1981 aggaataaac acgttgcagt ggcggacgtt ggccgatttc gcttcggtca cagatataac
     2041 cactcctgtc acgcattaaa tcaacactct caaagattct ggtgcggtat ttctccagct
     2101 cgttccccca gagcgtatat tctccgttcc ggttgtgaag cagaaggtaa caggtcattt
     2161 ccccgttacc aggcatggga gcctcgccat accccgtaac gactgctctc agcacccctg
     2221 aatccataac ggcattgtca tttttcctga gctgctccgc gcgactacgt tcagtatctt
     2281 cctgttcctt ccggcgcaat tcctcttcac gtttaagcaa ctgggctctg agagctgcaa
     2341 ggccggattc cccccactgc tgccactgtt tttcaaatgc catctgactc gcgggaatgt
     2401 cctgtttctc caccttccgg taatgctgtc gtaatttttc catttgctgg ctcagagact
     2461 ggatttttcc ctgctcacta cggtattccg gtgccgccca ttgcatcagg ggaatacggg
     2521 aaaaaccgga cgaaaacggc gatgattttt tcaggctggc agcatggctc atcgcttcct
     2581 ttcgttcggt gttcagctct tcccagcgtc tgcgcgtcgc ctgcatatct gccatcatcg
     2641 acatggcatt actggtgagt ttccatgccg agttactgtc cttcggcaac gcttcgataa
     2701 tgtcacgtag tgtgtacgaa gttgcgttgt caggaagccc gatattcaga aggtcagcca
     2761 cacggcgggc aggctctgtc agccctccgt tcctgagttc ggtgcgggaa atctgtattt
     2821 ccaggttaag cctgttaatc agacgattct gttcgccgcg ttctgtttcg ataccgcgtg
     2881 tttccatcgc acaggcagac tttccgaggt gaataccagg cgttttctca agcccctgtc
     2941 gctcgtatga acgatggtct atctcttcct gataaccggc gttcgccagt gccctgtttg
     3001 catggtcagc ccatgatgcc cgccacgtct cggcatgtgt ccggtcgttc cagtccctga
     3061 cctttccgct gaacccagca ggtccgatag ccctcgtggt cagcatgatg tgcgcatggg
     3121 gatttgtttt atccatgtga tggaacgcca catcagcaat catgccctga ctgacaaagt
     3181 tttctctgac aaaggcgaga acggtttccc gtgcggcatc ctgcggcagt tcgtggggga
     3241 tggcgagttc aatttcccgc gcaagttgtg agttcttccg gcgttcggcc ttctcaacgg
     3301 cattccagag ttcggcacgg ttcgtacacc aggcaggggc ggtggcgggg gtcagaatca
     3361 cagcctcagc cacaccgctt ttgcgggtgt agtcgtgagt cagtccggta cgctcatcaa
     3421 gcatcagtga accggaacga taggcagcgg aagctacggc gctgtagccg ctgcttctgg
     3481 agataatttt catgctgaga tgataaatcg ccatttctgc cgtaatgcct ttttgtagtt
     3541 ttctttcagc cccgcagggc gcacacaatc gaaacggagt ggagattgta taagtgcgca
     3601 cttcgtgatt tgccgtgcat tatgccagct cagtttttaa actaccagtg acgcgctaaa
     3661 atgcgatttt tcatcgcgca cggagttaca tcatgagcag aacgctcgaa cagaagattg
     3721 ctgatgctga ggcgagatta cagcgcctga aggcgaagag ccggagcctg gacacagcgc
     3781 agaaggttgt tgtgggtgct gcattgctgg caaaggtcag aaagccggag gaggtgcagt
     3841 tgcgtgcctg gttactccag ttcctgaagg cagaggtgac acgacaggct gatgtgacgc
     3901 gcatactgcc gctgattaac gagctggagg cgctgccaga acagtgattg tatcgtccgc
     3961 tcgccgcagc cccagcgacc gagcgttagc gagcgagtgg gcgaggaagc ggaagtgaat
     4021 cagtcgccag gttgagcaca tacgcacggt ttttcggcag gtgatatgcg ct
//
