LOCUS       NZ_CP049754             8937 bp    DNA     circular CON 24-JUL-2023
DEFINITION  Proteus mirabilis strain PmBR607 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP049754
VERSION     NZ_CP049754.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12995602
            Assembly: GCF_011149675.1
KEYWORDS    RefSeq.
SOURCE      Proteus mirabilis
  ORGANISM  Proteus mirabilis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Sato,J., Fonseca,M., Cerdeira,L., Sincero,T., Tognim,M., Noronha,M.,
            Lincopan,N. and Galhardo,R.
  TITLE     Proteus mirabilis ICE SXT/R391 genomic comparative analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Sato,J., Fonseca,M., Cerdeira,L., Noronha,M., Tognim,M. and
            Galhardo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2019) Microbiology, USP, Av. Professor Lineu
            Prestes 1374, Sao Paulo, SP 05508-900, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.11
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/24/2023 01:00:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,746
            CDSs (total)                      :: 3,637
            Genes (coding)                    :: 3,578
            CDSs (with protein)               :: 3,578
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 26 of 59
            Pseudo Genes (incomplete)         :: 32 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 13 of 59
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049754.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Proteus mirabilis"
                     /mol_type="genomic DNA"
                     /strain="PmBR607"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:584"
                     /plasmid="unnamed1"
                     /geo_loc_name="Brazil"
                     /lat_lon="23.556174 S 46.627129 W"
                     /collection_date="2015"
                     /collected_by="Mario C. Noronha do Amaral"
     gene            complement(join(8216..8937,1..388))
                     /locus_tag="G9C79_RS18695"
                     /old_locus_tag="G9C79_18695"
     CDS             complement(join(8216..8937,1..388))
                     /locus_tag="G9C79_RS18695"
                     /old_locus_tag="G9C79_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_152118482.1"
                     /translation="MASYHLSVKTGGKGKAASHADYIAREGKYAREKDNDLEHQESGNM
                     PAWAAHKPSEFWKATDTFERANGCTYREIEIALPRELTPAQRLELVRDFVQQEIGDRHA
                     YQFAIHNPRAAIEGGEQPHAHIMFSERLNDGIERDPQLYFKRANTKNPERGGAKKARFG
                     ETPTERKAYLIAQRERWADLQNKHLERYQHPDRVDARSLKAQGIEREPERHFGAGQVRQ
                     FNTAQLHALMERREAERQAQHCREECNSVIDVTTTLREALAERDNVTETPKCEVMQNAT
                     QGRTFDFEKESDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQDRVKQQEREYWA
                     EQRRQQEKERQRLAELKRQKPDDGWSFSR"
                     /name="MobA/MobL family protein"
     gene            554..1009
                     /locus_tag="G9C79_RS18700"
                     /old_locus_tag="G9C79_18700"
     CDS             554..1009
                     /locus_tag="G9C79_RS18700"
                     /old_locus_tag="G9C79_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_109880837.1"
                     /translation="MKNEKQAKWVADRVQYIRGLKSPNEAQRLLVILHDKADKTAQEIK
                     TLSLLIQAERAAEKAQEARTKVMNLIQAEKRAEAKAARKARDHALYQSAGLLIMAGLVD
                     SQTGKPVDDPAKLLGALVSLHELSRDNPKWEEWKLKGQALLNQKKED"
                     /name="conjugal transfer protein TraD"
     gene            1601..2557
                     /locus_tag="G9C79_RS18705"
                     /old_locus_tag="G9C79_18705"
     CDS             1601..2557
                     /locus_tag="G9C79_RS18705"
                     /old_locus_tag="G9C79_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152118481.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILCGVALLNPTIDDPNEDQ
                     RTVAFTYQQYAQMMGLSNDNAYQRLKNITRELMTRTIEIIYPTGPISERIFQWVNYAEF
                     NRDKQAVKLRFSKEIVPYLFQLKRFIKYNLEHVKAFENKYSMRVYEWLLKELTQRKTNK
                     ANIEISLKEFKFMLMLEKNYPEFKKFNHWVLKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELAKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTSFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            2565..2990
                     /locus_tag="G9C79_RS18710"
                     /old_locus_tag="G9C79_18710"
     CDS             2565..2990
                     /locus_tag="G9C79_RS18710"
                     /old_locus_tag="G9C79_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_109880842.1"
                     /translation="MALVSVTEAARLTGKNRATIHRYIKQGKLSQISDATNTKKVDTSE
                     LIRVFGSLTATHATLSQSDAMQHDATPKTDQSVALLQQKIALLEQLLESKNKELQRQDE
                     HIDSLKQAMLLIESKLPTTPEQARPVPAKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            3371..4165
                     /locus_tag="G9C79_RS18715"
                     /old_locus_tag="G9C79_18715"
     CDS             3371..4165
                     /locus_tag="G9C79_RS18715"
                     /old_locus_tag="G9C79_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109880844.1"
                     /translation="MLVFEIMVPGTWLDYDDSDWTWKIQNQLYSLQSQFFEANLALNLF
                     ISAQSSRAQLFSKDTWDTDSKRRREISEILEQEYIKQGKNYWESHEEISLQTDIIFKRE
                     KWQQGAIPREFEHNIAFLYARAFLYALDAFDKFLGVLSKEENVPEVISELHKKISDIFP
                     DLRGVRNTAQHLEDRSRGLGAGKKPKPLDLKPIENNFINAPNGGALVLNSLNGSRYGST
                     MADGHYGEVDVSPESMSHLQEILNNILGSFKWRGPKQHKPSV"
                     /name="hypothetical protein"
     gene            complement(4274..4564)
                     /locus_tag="G9C79_RS18720"
                     /old_locus_tag="G9C79_18720"
     CDS             complement(4274..4564)
                     /locus_tag="G9C79_RS18720"
                     /old_locus_tag="G9C79_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_109880846.1"
                     /translation="MRTIKYTSAFKKDYKREKKGQHRNTVDTELQTVLELLQVDEPLPE
                     KYRDHALVSNWVGHRDCHIKPDLVLIYKKTEEGELLILKLVRLGSHSELAI"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(4551..4811)
                     /locus_tag="G9C79_RS18725"
                     /old_locus_tag="G9C79_18725"
     CDS             complement(4551..4811)
                     /locus_tag="G9C79_RS18725"
                     /old_locus_tag="G9C79_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_109880848.1"
                     /translation="MASNALVTTRINENIKHEATEVLATMGLTVSDAVRLLLTKVAKEK
                     ALPFDLWQPNAETITAMEEDLSKAKSFTSVKDLMADLNADD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            5023..5265
                     /locus_tag="G9C79_RS18730"
                     /old_locus_tag="G9C79_18730"
     CDS             5023..5265
                     /locus_tag="G9C79_RS18730"
                     /old_locus_tag="G9C79_18730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109880850.1"
                     /translation="MTVLRQEKNAIIEKLNTVFTGVTHHGGTGFIQPKKDGEMTEKIFL
                     RLSKVAILSALYGCLTKNQTVISGKNDGGNPPSFF"
                     /name="hypothetical protein"
     gene            complement(5434..5933)
                     /locus_tag="G9C79_RS18735"
                     /old_locus_tag="G9C79_18735"
                     /pseudo=""
     CDS             complement(5434..5933)
                     /locus_tag="G9C79_RS18735"
                     /old_locus_tag="G9C79_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /name="restriction endonuclease subunit S"
     gene            6051..6233
                     /locus_tag="G9C79_RS19130"
     CDS             6051..6233
                     /locus_tag="G9C79_RS19130"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_239452671.1"
                     /translation="MGRTTNAVVCSYTTLCEVTGVSRPTVGRSIKLLKEDNWIDTVKIG
                     NANAYCVNERVFWQA"
                     /name="replication/maintenance protein RepL"
     gene            6221..6421
                     /locus_tag="G9C79_RS19135"
     CDS             6221..6421
                     /locus_tag="G9C79_RS19135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254434365.1"
                     /translation="MAGVRNQRKYAIFSATVIASASEQENDYVEKAKQKMTYIPIIEPK
                     DIPVTGCEELPPPDQGDLPRT"
                     /name="hypothetical protein"
     gene            complement(6617..7105)
                     /locus_tag="G9C79_RS18745"
                     /old_locus_tag="G9C79_18745"
     CDS             complement(6617..7105)
                     /locus_tag="G9C79_RS18745"
                     /old_locus_tag="G9C79_18745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109880852.1"
                     /translation="MVLRVTVEKYITLASLMLATFIFSISSYADSVFFTEKYSKISNGI
                     QKKLESCQNIAIDGYSYRDCISSAIDDEDKYLGFLRAEIKQNIKNNNLILSEFDRDINC
                     NKNIINTILNNKGTMWPGVAMQYCAVSKDAALKKWSYFYDVMKGSEQYENNLKGIFNE"
                     /name="hypothetical protein"
     gene            complement(7183..7323)
                     /locus_tag="G9C79_RS19140"
     CDS             complement(7183..7323)
                     /locus_tag="G9C79_RS19140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226889039.1"
                     /translation="MSQLSYLEKLLDGVEVYWEPLDKVFNIYAGGDVPTDALSDIETII
                     S"
                     /name="hypothetical protein"
     gene            complement(7619..8203)
                     /locus_tag="G9C79_RS18755"
                     /old_locus_tag="G9C79_18755"
     CDS             complement(7619..8203)
                     /locus_tag="G9C79_RS18755"
                     /old_locus_tag="G9C79_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /protein_id="WP_109880854.1"
                     /translation="MSDEIKKIAALIGHQQAQEKRVAALIEEFQAESAKLSRQTAQLAQ
                     VIHELDSASGRMTDTIRQSVNAALTQVEKELKQTGLAQQKPAVNALNQVVTAANESVSA
                     MRREMSRYTWKSAIYIALTIFAVMGGCLWGMYYFINSGYDRIAKMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRQSYGDKKDTYLIIKQ"
                     /name="tRNA modification GTPase"
     Typing          1602..2545
                     /name="rep_cluster_1159"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.508"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..388)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="99.0"
                     /evalue="7.74e-78"
     Typing          complement(8220..8935)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.913"
                     /coverage="99.0"
                     /evalue="1.07e-104"
ORIGIN
        1 cagaaaacat aatatgcgcg tgcggttgtt cgccgccttc aatcgccgct ctggggttat
       61 gaatggcaaa ttgataagcg tgccggtcgc caatttcctg ttgaacaaaa tcacgaacaa
      121 gctcaaggcg ttgtgcgggt gtcaattcac gcggcagggc aatttcaatt tcgcggtagg
      181 tacagccgtt ggcacgttca aacgtgtccg tggctttcca aaactcggac ggtttatgcg
      241 ctgcccatgc gggcatattg ccggactctt gatgctcaag gtcgttgtct ttttcacggg
      301 catattttcc ctcacgcgca atataatcgg catgggaagc ggctttgcct ttcccgccgg
      361 ttttaacgct gagatgataa gatgccatgc tattttcgcc cgctgcaagc gcgcaccgtt
      421 tctaaacaaa gtgaagaaac gtctaagtgc gccctatcaa taaaaatgat gatagggatt
      481 gtagtggaat ttgacctgag ttgccaatca tgagcataat tatttcattc taaaaacagc
      541 atgagtggta aagatgaaaa acgaaaaaca ggctaaatgg gtggcagaca gagtgcaata
      601 tatccgtggt ttaaaatcac cgaatgaggc acaacgctta ttggtgattt tgcacgataa
      661 agcggataaa accgcccaag aaataaagac gctctcattg ttaattcagg cggaacgtgc
      721 agcagaaaaa gcccaagaag cccgcacaaa ggtaatgaat cttattcagg cagagaagcg
      781 tgcagaagcc aaagctgccc gtaaagcgcg tgaccatgcc ctttatcaat cggcgggatt
      841 actcattatg gcagggttag tggacagtca aacgggtaaa ccggtagatg accccgcaaa
      901 actactgggg gcattagtca gcttgcatga attatcccgt gacaatccca aatgggaaga
      961 atggaaattg aaggggcaag cattactcaa tcaaaaaaag gaagattaga acagggtgtt
     1021 aataccctct aaaacgccac aggaattccg tgacgaggtt ttattggctc gatgatggaa
     1081 tttatcaccc aatactgaaa acgtggctca gaagctcaga cagctccttc ctgattgagc
     1141 cgtgagcgaa gcgaacccaa tgacacggaa aaaacagcgg cgcagtggct ggatggtcgg
     1201 agacaaaaga aatgttcaac gagttgtccg agcgagcaaa gcgaggagct actgaggtgt
     1261 tttaggtctg tcttgaaaag gagcaaacgt tgtttgcgac atccttttaa ctgcggcaaa
     1321 tcgacggcag ttttctgatt tatccacaga gcggggatct attctttttt atcttttatt
     1381 ctttctttat tctagtgttt ttattttatt gattttaaac gcaaaaaaaa cagaaaggta
     1441 cagtaaaaat cggaattggt acagtaaaaa tcggataagc agcgcaaaag gtacagtaaa
     1501 aatcgaataa cccacagaaa aaccgaaaag gtacagttga aaaaaagcac aaaaccttta
     1561 ttgtaagtac agcgatttat aacccttcac cgaggttgct atgtctgaat tagttgtttt
     1621 caaagcaaat gaattagcag taagccgtta cgaccttact gagcatgaaa ccaaattgat
     1681 tttgtgtggt gtggcgttac tcaatccaac aattgatgat cctaatgaag atcaacgaac
     1741 ggttgcattt acttatcagc aatacgcaca aatgatgggt ttatcaaatg ataatgctta
     1801 tcagagatta aaaaacataa ctcgagaatt gatgactcgc accattgaaa taatctatcc
     1861 aacaggacct atatctgagc gaatatttca gtgggtaaat tacgcggagt ttaataggga
     1921 taagcaagcg gtaaaactta gatttagtaa ggaaatagtg ccatatttat ttcaattgaa
     1981 aagatttatt aaatataacc tagagcacgt aaaagccttt gaaaacaagt attccatgcg
     2041 cgtctatgaa tggttattaa aggaattaac tcaacgtaaa accaataaag ctaacattga
     2101 aataagcctt aaagaattca agtttatgtt gatgctagaa aaaaattatc ctgaatttaa
     2161 aaaattcaat cattgggttt taaaaccagt tacaaatgac ttaaacactt acagcaatat
     2221 gaagttgact attgaaaaac gcggtcgtcc tgctgacaca ctgatctttc aagttgaact
     2281 ggataaacaa attgaccttg tgactgaact agcaaaagat cccgcatcaa aaaaagaaga
     2341 taagacaatc cgtttaacgc ctgaaaatcg tcttcatgag gggctaaaaa caacattgca
     2401 tgatgcttta actgcaaaaa ttcaactgac tagttttgaa gcaaaattcc tgagcgatat
     2461 gcaaagcaag tacgatctta acggttcatt tacatggctg actcaaaaac aacgaactac
     2521 gctagagaaa attctggcga aatacgggcg gatatgaggt atctatggca ctggtatcag
     2581 ttacagaggc agccagacta actggaaaaa acagagcaac aatacatcgc tatataaagc
     2641 aaggaaaact gtcgcaaatt tctgatgcaa caaatacaaa aaaagtagat acatcagagc
     2701 ttattcgtgt atttggttca ctaactgcga cacatgcaac actgtcgcaa agcgacgcaa
     2761 tgcagcacga tgcaacacct aaaacagatc aatccgttgc actgttacaa caaaaaattg
     2821 cacttttaga gcaactgctt gaaagcaaaa ataaagaatt acaacgtcag gacgagcata
     2881 tagacagcct gaaacaagca atgctattaa tcgaatccaa gctgccaact acacccgaac
     2941 aagctagacc tgtgcccgcc aaaaaatcat ggcaattctg gaaaaaatag ccggttggtg
     3001 tcattttcac cgcgaaaccc aaaccgctat tttaataaca aacgagcaac attaaaaatg
     3061 gtgtttttta cggtagaaaa gaagtggttt tagccagttg atggcacttt ttgattgagt
     3121 gcctaagtcg cattacgctt aagcatgttt atgttaaaca gaaccgattt tcggctaaat
     3181 ttaaagatgt aaacgcaaca tggacgcatt ggactcgttg tagataacca ttatctatag
     3241 gagaacgatg agtcacttca attaccgaaa agattaatac cgtctttatt ctcgtaagta
     3301 taagattatt aaaaaacata tccaatgtct gataagttat ctctttataa aaaggaaata
     3361 ggagagcatt atgcttgtat ttgaaattat ggttccaggg acttggcttg actatgacga
     3421 ttcagattgg acatggaaaa ttcaaaatca actttattca ttacagtctc aattttttga
     3481 agctaatcta gcattgaatc ttttcatatc agctcaatct tctagagcac agttattttc
     3541 caaagatact tgggatacag attcgaaaag aagacgagaa attagtgaga tattagaaca
     3601 ggaatatatt aagcagggga aaaattattg ggagagccat gaagagatca gtttacaaac
     3661 agatattatt tttaaaagag aaaagtggca acaaggagca ataccaagag agtttgaaca
     3721 taatattgca ttcctttacg ctcgtgcatt tctttatgca ctagatgcct ttgataaatt
     3781 tctaggggta ttaagcaaag aagaaaatgt acctgaagtt atttcagaac tacataaaaa
     3841 aatatcagat attttccctg atttaagagg ggtacgtaat acagctcagc atctagaaga
     3901 tcgttctaga ggactaggag ctggaaaaaa accgaaacca ttagatttaa aaccaattga
     3961 gaataatttt ataaatgctc ctaacggtgg tgcattagta ctcaatagct taaatggttc
     4021 taggtatggt tcaactatgg ctgatggaca ttatggagag gttgatgttt ctccagagtc
     4081 aatgagtcat ctacaggaaa tccttaataa tattttagga tcatttaagt ggcgtggtcc
     4141 caaacagcat aaacctagtg tttaattaga tttcctaaag ttaacacaat acatcttata
     4201 cgaaatcaaa aatgggagcc tttagctccc atttttacta tatttttaaa ttttattgtt
     4261 gaaattaagc cgtttaaatt gctagttcgc tatgacttcc taatcgaact aatttcaaaa
     4321 ttagcagttc accttcttct gttttcttat agattaaaac aagatcaggt ttgatatgac
     4381 aatcccgatg acccacccaa ttactgacca aagcatggtc acgatacttt tcaggcaaag
     4441 gttcatcgac ttgtagtaac tctaaaacag tttgtagttc ggtatctact gtatttcgat
     4501 gctgaccttt tttctcacgt ttgtagtctt tcttgaatgc tgatgtatac ttaatcgtcc
     4561 gcatttaggt cagccatcaa gtcttttaca gaagtgaagc tctttgcttt acttagatct
     4621 tcttccattg cagtaatggt ttcagcattg ggttgccaaa ggtcaaaagg caaagccttt
     4681 tctttagcta ctttggtcaa taacaaacgg actgcatctg agactgttaa gcccattgta
     4741 gctagaactt ccgttgcttc atgcttaatg ttttcattga tgcgagtagt cactagtgca
     4801 ttcgaagcca tatcaagccc cctgattaac attgttaatc gaattatatt cccagatgag
     4861 gaaaatttca attcgcttaa gcacgattat gttaaacaaa gctgattttc agctaaattt
     4921 aggtaataag aatgggtaga tgtaaacgca acatggaggc attggactcg ttgtagatat
     4981 tcattatcta caggagaacc atgagtaatt ttaataaaac ctatgaccgt acttcgacag
     5041 gaaaaaaacg caattatcga aaaactcaat accgttttta ctggcgtaac ccatcatggt
     5101 ggaaccggat ttatacaacc aaaaaaagac ggggagatga cagaaaaaat ctttttaaga
     5161 ttatcaaagg ttgcgatcct gagtgcattg tatggatgcc tgacaaaaaa ccagacagtt
     5221 atttctggta aaaatgacgg cggaaatccg ccgtcatttt tttaagatta acaacttgtt
     5281 catgagtagt gatttttcgt tcctcaacct ttgaggatat caataattat tttttctttt
     5341 gccgcttctg gcagaatatt atttgttaat ttataaaaaa actcataatg atactggtga
     5401 acactcatgt gtcgagatta tccgagcgtt aaattaactc tctagtgtcc ctagtttagg
     5461 aaagttcaac aacaggtcac gataatactc gtattgcttt tggcgtaact cgatttcgcg
     5521 tggaagacct tcttgcagtg aagttgttaa tgtgtcgaat ttatccaaaa ttcgcacgat
     5581 ttcagattgg attgccagcg acttttcggg ggtatctggg caggggatgg ggataacaac
     5641 atttttaagc attggttttg taagttgagg aatacctgcc ggagcaactt tataattatt
     5701 ttcataaagt ggtaagcata cttcaggttc aattcaactt taggtgtcag caccaataaa
     5761 cgaactatag gaaaaaatgg cctgtctcta taactagtcc atcctattgt tcctcttgct
     5821 gatatagtta aacttggctt ttcgattttg gcttggtttg tccaaccata aagagattta
     5881 tcaccaattc cgatagaaag aatagggata ttaaattcct caatgagtct cacagtgaaa
     5941 atgataattc taactttgtg cagctttctc gtggctacct tcaacagatg cgtaagttag
     6001 ctcgcaaaag ccccatagcg cacgaaattc tgtattacct agtggagcac atgggtagaa
     6061 ctacaaacgc tgttgtgtgc agttatacaa ctctatgtga ggttactggt gttagccgac
     6121 caactgttgg aagatcaata aagcttctta aagaggataa ttggatagac acagtgaaaa
     6181 tcggcaacgc caatgcttat tgcgttaacg agagggtatt ttggcaggcg tgagaaacca
     6241 aaggaaatac gctattttct ctgcaactgt aattgcgtca gcttcagaac aagaaaacga
     6301 ttacgtagaa aaagcaaaac agaagatgac atacattccg attattgaac ctaaggacat
     6361 tcctgttact ggatgcgaag agcttccacc accagatcag ggtgatcttc cccgcacata
     6421 gaaattcgga cactcaatac tggcagaaaa acacctgatt ttgccgatgt atgagtacgg
     6481 agagtaataa gatacagaag gggatcgctc agaagccgcg ccacaagcgg aaagtttttg
     6541 acaatagtta tttatcaaga taactatttt tcctgaaatt ggagattttt agatgcggag
     6601 aatttagagc taaatattat tcattaaaaa ttccttttag gttattttca tactgttcgg
     6661 aacccttcat gacatcataa aaatacgacc attttttcaa tgcagcatct ttactgactg
     6721 cacaatattg catagcaaca ccaggccaca tagttccttt attattaagt attgtattta
     6781 ttatgttttt attgcaatta atgtcacgat caaattcgct taagataagg ttgttatttt
     6841 ttatattttg ctttatctca gctcggagga atcctagata tttatcctca tcatcaatag
     6901 cactagatat gcaatcacga tagctataac catctatagc tatgttttga cagctttcta
     6961 gttttttttg tattccgttg cttatctttg agtatttctc ggtaaaaaat acagaatcag
     7021 catacgacga aatgctaaaa atgaatgtag ctagcatcaa gctggctagg gttatgtatt
     7081 tttcaaccgt cacacgaagc accatgtaga cccctaattt agatatagaa aggaacaaac
     7141 cgcaaccaat tgatatttaa atcatatcat agaagggtag tctcatgata tgatagtttc
     7201 aatgtcagat aaagcatctg taggtacatc acctccagca taaatattaa acaccttatc
     7261 taaaggctcc caataaacct caaccccatc caagagtttc tctaaatagc tcaactgact
     7321 catgcctcaa cctcctcacc ttcaatttcc gcgactatgg catcaatatc actgcgaagc
     7381 gcatcaattt gaagttcggc acgttgttgt aaacttgtca tctattaatt cctgcttttc
     7441 attcgttata attcgtttat gggttgcgca atgtccacta ccgttgaaac atcatccgtc
     7501 aaaccctagg gctaaatgag atcatcagac tggtcgggat taagttcttt gccaacagtc
     7561 agatggacgc attcgttgac gacttgcacg ggaaaacggc gtgttcagcg ccgttttttt
     7621 attgtttgat aatcagatag gtgtcttttt tatcaccata tgactgcctt gtatccactt
     7681 tcacacaagg ttttccatca cacgtggaga tatcagccag cggggctttt ttttgccaga
     7741 ccgcttccat gcgctgcatt tttgcaattc ggtcataacc gctgttgata aaataataca
     7801 ttccccaaag acacccgccc atcaccgcaa atatcgtcaa tgcgatataa atggcagact
     7861 tccacgtata acgggacatt tcacgtcgca ttgcactcac ggattcatta gctgctgtca
     7921 ccacctgatt cagcgcattg acggcgggct tctgctgcgc caatcccgtt tgtttgagtt
     7981 ctttttcaac ctgagttaaa gcagcattca ccgattgcct gatcgtgtcg gtcatcctac
     8041 cggaggcact gtccaattca tggatcacct gagccaactg tgctgtttgg cggcttaatt
     8101 tagcggattc cgcctgaaat tcttcaatca acgccgccac acgcttttcc tgcgcttgct
     8161 gatgcccaat cagcgccgca atttttttaa tttcgtcact catacaaaat tctctctatc
     8221 gtgaaaatga ccagccgtca tccggtttct gtcgtttcaa ctcagcgagg cgctgccgtt
     8281 ctttttcctg ctgacgccgc tgctcagccc aatactcacg ctcctgctgc ttaaccctgt
     8341 cctgatgaat gccctgaaac tccgccatcg catcgttaac aagggactga agatcgattt
     8401 cttcctgtat gtccttcatg gcatcactga ccagtgcgtt cagcttgtca gactcttttt
     8461 caaaatcaaa cgttctgccc tgagtcgcat tttgcatcac ctcgcattta ggtgtttcag
     8521 tgacgttatc gcgctcagcg agggcttccc gtaatgtcgt cgtgacatca atcacgctgt
     8581 tgcattcttc acgacagtgc tgagcctgtc gctccgcttc tctgcgctcc atcagggcgt
     8641 gtaactgcgc tgtattaaac tgcctgacct gtcctgcgcc aaaatgtcgc tccggctcac
     8701 gctcaatccc ctgtgctttc agggaacgcg catcaacgcg gtcgggatgc tgataccgtt
     8761 caagatgctt attttgtaag tcagcccagc gttctcgctg ggcgataagg tatgccttgc
     8821 gttcggtcgg cgtttcgcca aaacgagctt tttttgcgcc gccccgttca gggtttttcg
     8881 tgttagcccg tttaaaatac aactgcgggt cacgctcaat accgtcatta agccgtt
//
