LOCUS       NZ_CP049295             2046 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 08-175 plasmid
            pHS08-175-6, complete sequence.
ACCESSION   NZ_CP049295
VERSION     NZ_CP049295.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14142282
            Assembly: GCF_019355415.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Guo,Q.
  TITLE     Enterobacter hormaechei subsp. steigerwaltii 08-175 producing SFO-1
            and CTX-M-125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Guo,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Institute of Antibiotics, Huashan Hospital,
            Fudan University, 12 M. Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.5.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 656x
            Sequencing Technology             :: Illumina, PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019355415.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:24:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 127
            rRNAs                             :: 11, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 37 of 108
            Pseudo Genes (incomplete)         :: 73 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049295.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="08-175"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pHS08-175-6"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.2171 N 121.44384 E"
                     /collection_date="2008-11-14"
                     /collected_by="Institute of Antibiotics"
     gene            complement(join(1438..2046,1..408))
                     /gene="mobV"
                     /locus_tag="G6324_RS24970"
                     /old_locus_tag="G6324_25015"
     CDS             complement(join(1438..2046,1..408))
                     /gene="mobV"
                     /locus_tag="G6324_RS24970"
                     /old_locus_tag="G6324_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_089622504.1"
                     /translation="MRSPDWLDGRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENHCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEAT
                     PRQQAEFFAHSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            940..1374
                     /locus_tag="G6324_RS24975"
                     /old_locus_tag="G6324_25020"
     CDS             940..1374
                     /locus_tag="G6324_RS24975"
                     /old_locus_tag="G6324_25020"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2..408)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.529"
                     /coverage="100.0"
                     /evalue="4.6e-89"
ORIGIN
        1 gtcccggtga actaccgcag ccacaacacg atccttcccg tatttcttct ccagccactg
       61 ctcagaatgg gcgaaaaact ccgcctgctg ccgcggtgtc gcttctttcc accactcagg
      121 gcttgccgtc ataacgtatt caacagccag taccgcatct ttacggcgtt tttccggcaa
      181 cagctcccgc agctttccca tcgcctcatc tgccgactga gacacagagc agtggttttc
      241 tggtgtccgt tcagcgtcag cattcggcgt ctcacgctcc ctgtagcagt gctgcaaagc
      301 gctggccaca ctgcccattc ccgtcagctt tttgcagcgc attatcgcgt aattcgccat
      361 gcttaccccc tcgttctggc gtccatccag ccagtcgggg gatcgcattg cgatgcctcc
      421 ggcgggccac tatcagattg atagtgtact cactagactt tgctacgcaa aatcgttcgg
      481 gcagcttgct gccagaaggt caaaatcaac ggcggtctgg caggtggaag gcgtcgctcc
      541 gcgtcacctc ccaccactgc caagcctgtt ttatttgtgc gtcgtcaagg gtgaaatgca
      601 ctcgctacgc tcgcccttga cgaaactagg caatgaatat cgcttttgaa agagcaggaa
      661 ccgctcgccg caggtcaggc tgaggcgtac cgcagcgcag cgaggaacga acagcctgaa
      721 cgaggaagcg gaatatcacc tgaacgggca tagaaatgct tacgcacgaa ccgatgaaaa
      781 tagggttcgc tatccgggca tatgagcttc tcgctataag atacagaaga gagccttctc
      841 tcagcccttg tggggcgcgg ctctcgcttg acgatagtta tcattattga taacgttgtt
      901 atcattattg ataactattt ttttgggaga gcctcttaaa tgagtaaccg aattactcag
      961 atagacactg ctacagggga agaggtaggt agttttgtcg cagttatccg acccaaacag
     1021 aagtcagctt ttgagaggca tttcactatg aaccaagacg ctttaaagat ccttgctaag
     1081 accctcacag gggagcaatt caaagtctta atgatgctgt tagctgatct cgattatgaa
     1141 aattacatcc aaattgccca agccgatatt gccgacgctt taggtatgca gaaaaccaat
     1201 gtaagccgtt cggttaaggc gctaattgac ctatccgtga ttcttcaagg cccaaaagta
     1261 gggcgcagtt acagttaccg tctgaaccct cagtttggct ggaaaggtac tgtcagcaac
     1321 cacaaaaaag cactgaaaaa cggcctcagc gttatacagg gcggcagaac ctgatctctg
     1381 gaatgccgca aatggcgtca ttttgcggcg ttagccctat cctgcatcgc atcaaaacta
     1441 acgctccata ccgcccatgc cacgcgatcg catctgtcgc tgtttctcac gttcaacacg
     1501 ctgctgtttc tccttctcct ggttttgctg cttaaaccgc tgagccatca tcaggatttc
     1561 tgtcatctgc tctctggaaa gccccttaaa aggctctgta acgctctgta ggcgtttctg
     1621 ctgcgaatcc atagtcttct gtagctcgcg cgctcttttt gcattctggg cgctctgaga
     1681 ggccatagcg atggttcctg caaagccatc gttcactgcg tggcttagac gtttggctat
     1741 ggctgcctga tcttccacgt ctttggtgaa aatgcccttc cgcaatacac gcggctccag
     1801 cgcctccggc gagatcgata cctggctacg ggtgccacgg tttatcgatt cgtagtagtg
     1861 ctgaactgtc tgatgcgtgg cgcggcttcc ctcgatgccg cgctcaagac ccagtttttt
     1921 tactgattca gcgtaggtgc tctgatcgtc acgcatcttt gaccgcccgc caataaactc
     1981 tttcgcggac agccgtccat cctgcgtcaa tggcaccacg aacgctgaca aatgcggggt
     2041 ggcctc
//
