LOCUS       NZ_CP049294             2175 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 08-175 plasmid
            pHS08-175-5, complete sequence.
ACCESSION   NZ_CP049294
VERSION     NZ_CP049294.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14142282
            Assembly: GCF_019355415.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Guo,Q.
  TITLE     Enterobacter hormaechei subsp. steigerwaltii 08-175 producing SFO-1
            and CTX-M-125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Guo,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Institute of Antibiotics, Huashan Hospital,
            Fudan University, 12 M. Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.5.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 656x
            Sequencing Technology             :: Illumina, PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019355415.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:24:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 127
            rRNAs                             :: 11, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 37 of 108
            Pseudo Genes (incomplete)         :: 73 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049294.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="08-175"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pHS08-175-5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.2171 N 121.44384 E"
                     /collection_date="2008-11-14"
                     /collected_by="Institute of Antibiotics"
     gene            612..1037
                     /locus_tag="G6324_RS24960"
                     /old_locus_tag="G6324_25005"
     CDS             612..1037
                     /locus_tag="G6324_RS24960"
                     /old_locus_tag="G6324_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_015328245.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            complement(1303..1539)
                     /locus_tag="G6324_RS24965"
                     /old_locus_tag="G6324_25010"
     CDS             complement(1303..1539)
                     /locus_tag="G6324_RS24965"
                     /old_locus_tag="G6324_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(1704..1876)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          2..366
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.759"
                     /coverage="99.0"
                     /evalue="1.92e-72"
ORIGIN
        1 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
       61 cccccgttca gtccgactac cacgcccgtt ccggtaacta tcaacttgag tccaacccgg
      121 aaagacacga caaatcgcca gtggcggtag ccattggtaa cggatttaga ggagttagtc
      181 ttgaagttat gcgcctgtta aggctaaact gaaaggacag atttggttac tgcgctcctc
      241 caagccagtt accgcggttc aaagagttgg tagctctgag aaccttcgaa aaaccgcctt
      301 gcagggcggt tttttcgttt ccagagcaag agattacgac gcaacggaca agatctcaag
      361 aagatcatct gtgatattcg acagatatgg gataaaggat tgcaacgcca agagtatatg
      421 agacatagca gcagccacca ccaccatcac agaatcaagc cagttgcggc acgaaaggaa
      481 agaaccgaac tgtgcgacgg tcgctacgct ctccgtaaat tttgtatacc taccacggag
      541 acataaagtt tctattgctg attcgcctta aaaagggaaa aatcctattt tttctaaatg
      601 gatataacct tatgggaagc tacgtagacc aatcattgac aagaaatgaa tccgtaatat
      661 ctcgcgctca aacctcctgg attccaacaa tcatcccagt aattatcgga atcttgttgc
      721 tgccttttta cggcttaggg ttacttataa taatccctgt gctccttaga gtttggtcaa
      781 cggagcttgc attaactaac caaagagtca tcgcaaaagt gggtttgatc cgacgcaata
      841 cggtggaact ccgtattgat aaagttgaaa gccttggcat acatcaaggg atactgggac
      901 gaatctttaa ctatgggtct atcgtgatta aaggcactgg cggaaccaac actccaattc
      961 caaacatcaa ggcgccaatg caattcagat cgattgtgaa caatcatatc gaggaaatga
     1021 attctaagca gcagtagctt cctcgtagca ttacaaaaaa agccctacgg ggcttttttt
     1081 gtacctgcca atcagctgga tttaccataa cacccgttgc ccgcaccgcc tcagaggctc
     1141 tcagggcgtt tctccgccct aatacgcaaa agaacacgta tgacctcctg aaaaacgttc
     1201 agaaacgctc tggtgatttt tgagcgcatg aatcgttatg cacgcgggtg aatcgcaggt
     1261 gatttcgcag cgtcagcacc actaaaccga caaaatccgc aatcagcggc gcggcgcgcg
     1321 cggacgtgac caggcaaaat cggactgctg ccgctcatcc tcacgtaatg cacgctggcg
     1381 gtcggcgtaa cgctgcgact caatctgcgc acgcgctctg ttcagaacga tgtgccggtt
     1441 aggctgattg agcttcttcc cggccgcacg ttcggcagcg gctatagcgt cgtcgataaa
     1501 ctttttctca gcgggggtca ggtttgtaag tctcgtcatg tttatctccg tatgagaata
     1561 gtcccagcag tctgccagac acagtaggaa cgatgaagcg taagcgacgg tgaaaggcag
     1621 tcagaaacgg tgaacgggag cggattttaa tccggtgccg tgaggcgttg gacaagccgc
     1681 aggcgcgtca gtggatttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct
     1741 agcggagtgt atactggctt attatgttgg catgagtgcg gtaatcagga aagtgcttca
     1801 tgtgacagag gaaaatgcag ctgcgggcgt aaggcagaaa atggaagttc agaggatatg
     1861 acgcctcctc gctcactcag tcgcggacgc tcctgccgtt cggctgcggc gggcgttacc
     1921 tgcacacgga gaagagcaaa ggcatatgcc atgacaaacg gggcagaaaa aggatgcggc
     1981 gctgccgttt ttccataggc tccgcccccc tgacaagcat cacagaatct gacgctcaaa
     2041 tcagtggtgg cgaaacccga caggactata aagatcccag gcgtttcccc ctggtagctc
     2101 cctcgtgcgc tctcctgttc ctgcctttcg gtgtaccggt gtcattccgc tgttatggcc
     2161 gcgtttatct cattc
//
