LOCUS       NZ_CP049293             2746 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 08-175 plasmid
            pHS08-175-4, complete sequence.
ACCESSION   NZ_CP049293
VERSION     NZ_CP049293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14142282
            Assembly: GCF_019355415.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Guo,Q.
  TITLE     Enterobacter hormaechei subsp. steigerwaltii 08-175 producing SFO-1
            and CTX-M-125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Guo,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Institute of Antibiotics, Huashan Hospital,
            Fudan University, 12 M. Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.5.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 656x
            Sequencing Technology             :: Illumina, PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019355415.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:24:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 127
            rRNAs                             :: 11, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 37 of 108
            Pseudo Genes (incomplete)         :: 73 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="08-175"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pHS08-175-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.2171 N 121.44384 E"
                     /collection_date="2008-11-14"
                     /collected_by="Institute of Antibiotics"
     gene            839..1066
                     /locus_tag="G6324_RS24940"
                     /old_locus_tag="G6324_24970"
     CDS             839..1066
                     /locus_tag="G6324_RS24940"
                     /old_locus_tag="G6324_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223229632.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHDGYT"
                     /name="hypothetical protein"
     gene            complement(1114..1302)
                     /locus_tag="G6324_RS24945"
                     /old_locus_tag="G6324_24975"
     CDS             complement(1114..1302)
                     /locus_tag="G6324_RS24945"
                     /old_locus_tag="G6324_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1496..2005
                     /locus_tag="G6324_RS24950"
                     /old_locus_tag="G6324_24980"
     CDS             1496..2005
                     /locus_tag="G6324_RS24950"
                     /old_locus_tag="G6324_24980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045270081.1"
                     /translation="MFNLAVQHSYHRTYRPLVVFRTIFICIICILLYYFFKLERWGNNN
                     MNGMSDVRLKKSTIESLKEYVNDFGMTVDDVVLNLIEENNSLKSLLGQGFSLPGGDKDT
                     HSFKDYVIHKLPKGKILVTKDGLKVSNMKKVLIEAGSAVGMDDKELNPDFTTYEIGRFL
                     FKHLQG"
                     /name="hypothetical protein"
     gene            complement(2194..>2295)
                     /locus_tag="G6324_RS24955"
                     /old_locus_tag="G6324_24985"
                     /pseudo=""
     CDS             complement(2194..>2295)
                     /locus_tag="G6324_RS24955"
                     /old_locus_tag="G6324_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     Typing          complement(2..366)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.175"
                     /coverage="82.0"
                     /evalue="1.5000000000000002e-168"
     Typing          complement(2617..2746)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.697"
                     /coverage="93.0"
                     /evalue="5.65e-50"
ORIGIN
        1 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc ggccataaca
       61 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
      121 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc caccactgat ttgagcgtca
      181 gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcttcg ccgtggcctt
      241 cttgcttccc tgttaagctt cttcctggaa gatgccagga aatttccgcc ccgccagtaa
      301 gccggtaccg ctcgccgcag ccgaacgacc gagcggagcg agtcagtgag cgaggaagcg
      361 gaatatctcc gggcttatct ttggcaccgt acgcgccata gctgcatttt ttaatacgat
      421 gcaggatagg gcaaacgccg caaaatgacg tcatccgaca tcattccgcc gcgttcgtcc
      481 agtgacctac agtcggctct ggggtgtcgg ggctgtgccc tgaccaggcg ttttggagta
      541 tcgccaccgt ggtggcggtg ctacaaaaca tatcctgtcc cttccttggc atctctgaag
      601 tttagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg ccccgacacc
      661 cgctaaaagc actgacgcgc ctgcggcttg cccaaccccg cgccgtccgg gaaagaattt
      721 cccgatcgac ccgacgtttc tgtttacctg tgatgttaac gtttggatct ttttcccggg
      781 tttacctatt ttcttctaat gaagcgttca cagctgacgt taatagatgg gtgctttcat
      841 gtttggtttt tcaccaggag aacttatgaa cctttgcccc gatgaacgtc tgctctttgt
      901 gcggatgatt tccgctatgc ttcgccgttc gggcggtgat gcgggtgctg ttatgtttga
      961 ggcgtatcgc catattgtgt ccgataccaa tcaagctcgt cgttcctaca tgctcgacct
     1021 gctcgaaagc gtcaggcatg actatgtaca cgatggatat acatagttgc cctggatacg
     1081 cttttcgtct aagtgagcgt tcttttcgtc tctttattcg ccgtcctgta taccgaagcg
     1141 tgcgctcagc ttctgataaa gcgcttctgc ctgttcgtgc attcgctcac agtcggctgc
     1201 ctcgtcatca agatcgagca cgtcgagttt ttcgagcagc agcagcgact gcgcccggat
     1261 aaattttgcc atgttcagtg cggctgattc ctgttttgtc atacggcttt ttcccggttt
     1321 tatggtgatc gcagactgaa attttacttc attcacttga gtgcatagcc atgcaggtac
     1381 gcagaaatca tcagcgcgtt tctgagcggt tttcaggggg taagatgtgt tcttttgcag
     1441 gtgaggtcgt agaaacgccc tgagagcctc tcaggcggtg cagacaacgg atgttatgtt
     1501 caatctggcg gtgcagcata gttatcatcg cacctacaga ccgcttgttg tattcagaac
     1561 aatatttatt tgtataattt gtattttgtt gtattatttc ttcaaattag agcggtgggg
     1621 taataataat atgaatggta tgtctgacgt aagactaaaa aaatcaacta ttgagagcct
     1681 aaaagagtac gtgaatgact ttggcatgac ggttgatgat gttgtcctga acttaattga
     1741 agagaataat tcattaaaat cgttattagg tcagggcttt tctttgcctg gtggagataa
     1801 ggacacacat tccttcaagg attacgtgat tcataagctt ccaaaaggaa agatcttggt
     1861 taccaaagac gggcttaaag ttagcaacat gaagaaggta ttgatcgagg cagggagcgc
     1921 tgtagggatg gatgataaag agctaaaccc tgactttacg acctatgaaa taggccgttt
     1981 tctcttcaaa catcttcagg gataaaggtg tctaccagtt cgtggccggg attttaggtt
     2041 caattcagct agttcccgga cagaaaaaaa gccaccgtgt aggtggcttt tttattcctg
     2101 ctttaggcaa agcattattg atagcatttg caagcaaacc gttagcgact tctgctgaat
     2161 gcttctgggc ggattcaagt tcgaagtgca acgctaagcc gcctgtctta gctcagcaaa
     2221 gaccactgaa ggttgccgga atccaagaca tttccgtggc ctgctattca gtgctctctg
     2281 gtacttgcgg agctcccctg tgtgataagt caggtgtgga atttttacgt ataaggcccc
     2341 gctaggcgga aaccaatact gaagccaaat cactggtaaa cagccggatc ttcttgtgat
     2401 ccttttactc tcgtcgtaat ctcatgtcct gtaaacaaaa aaaccaccgc taccaacggt
     2461 ggttcgtttg ccggaatcaa gagctaccaa ctctttcttc cgaaggtaac tggctttcac
     2521 ggagcgcaga taccaaaact gtccttccag tgtagccgca gtcaggccgc cacttcaaga
     2581 actctgtaga agcactctac taccctgctc cgtgaatcct cttaccaatg gctgctgcca
     2641 atggcgttaa gtcgtgtctt tccgggttgg actcaagacg atagttaccg gacaaggcgc
     2701 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttgga
//
