LOCUS       NZ_CP049292             4934 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain 08-175 plasmid
            pHS08-175-3, complete sequence.
ACCESSION   NZ_CP049292
VERSION     NZ_CP049292.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14142282
            Assembly: GCF_019355415.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Guo,Q.
  TITLE     Enterobacter hormaechei subsp. steigerwaltii 08-175 producing SFO-1
            and CTX-M-125
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Guo,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Institute of Antibiotics, Huashan Hospital,
            Fudan University, 12 M. Wulumuqi Road, Shanghai 200040, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.5.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 656x
            Sequencing Technology             :: Illumina, PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019355415.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:24:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,015
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,780
            CDSs (with protein)               :: 4,780
            Genes (RNA)                       :: 127
            rRNAs                             :: 11, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 37 of 108
            Pseudo Genes (incomplete)         :: 73 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            Pseudo Genes (short protein)      :: 1 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049292.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="08-175"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pHS08-175-3"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.2171 N 121.44384 E"
                     /collection_date="2008-11-14"
                     /collected_by="Institute of Antibiotics"
     gene            810..2096
                     /locus_tag="G6324_RS24915"
                     /old_locus_tag="G6324_24940"
     CDS             810..2096
                     /locus_tag="G6324_RS24915"
                     /old_locus_tag="G6324_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            3116..3439
                     /locus_tag="G6324_RS24920"
                     /old_locus_tag="G6324_24950"
     CDS             3116..3439
                     /locus_tag="G6324_RS24920"
                     /old_locus_tag="G6324_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3648..>4082
                     /locus_tag="G6324_RS25170"
                     /pseudo=""
     CDS             3648..>4082
                     /locus_tag="G6324_RS25170"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4117..4608
                     /locus_tag="G6324_RS24930"
                     /old_locus_tag="G6324_24960"
     CDS             4117..4608
                     /locus_tag="G6324_RS24930"
                     /old_locus_tag="G6324_24960"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4615..4830
                     /locus_tag="G6324_RS24935"
                     /old_locus_tag="G6324_24965"
     CDS             4615..4830
                     /locus_tag="G6324_RS24935"
                     /old_locus_tag="G6324_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2818..3003
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.513"
                     /coverage="100.0"
                     /evalue="9.410000000000001e-66"
     Typing          complement(2347..2893)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          3430..4928
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagcccgcac gaagaagggc tcatcgcgtt cctgctaaat aggctgacta ccggaatggt
       61 agtgacttat cccggaggaa gtgatgaaaa ttctcaacgt tattgaagcc ctgctgaact
      121 gtttttacct ggtgatggag ctgtgtgagt tgccggagag gcatttgccg gtttttaagg
      181 cgttcgcgct gattttagtg tgactttacg ctgcatgcat ctggctgcat gactatgcat
      241 cagtgtaata tccagcggct gcgtttttgg tgttttttcg tcatgtctgt cgccactttc
      301 tcgcgtctgg caccggaaac gcagccaacc ccgtttctgc ggtgcgggca aggggtgtta
      361 tgttaaaagc cgaggtctgg cggatacgga aaaaattatg cccgcggcaa tccggccgat
      421 acttaaatta tccttcacaa aggatgtgct gcgatccagg cagttgagtg ttttccggct
      481 cccggatggg agccattttt ttgccttctg atgcctgtta atgcatgcgg agctgcgctg
      541 gttcttccaa gattgataga cgccagcttc tctgctggag ccggcggatt tgccgtaaat
      601 atgaacattt tcttattgtt cagcccgcta aagatggcgg gttttttacg cgtgaaatcc
      661 ggagccggac atcatgccgg catggctgaa tagcgggctt tttcccgccc tgtcgcagta
      721 atatataacg ctacataagg agtagccgat aaccatggca gtacttacat tatccgggca
      781 atctgattca tatgttttca cttaaaaata tgttctccct gaacctccag cgatcagcca
      841 tttcgtatga cgcaatttgt cagaataagc ctgtcattga gttcagtccg gagggggtga
      901 taaataaagc cagcccgctt tttctctcca ctatggggta cagggctgat gaaataatcg
      961 gtcaccacca tcgtatattc tgcccgccct cgctggtaag ttcaccggaa tacagccagt
     1021 tctggcagcg ccttgccagg ggagaaagct ttagtggcaa gtacctgcgg ctggcaaagg
     1081 gaaatcgttc cgtatggctt gaagccagtt atatccccgt ttctgacagg cgcggccgtg
     1141 ttatcagagt cattaaaatt gctgccgata tttctgagcg cgtgcattct gctcttgagc
     1201 aggaagcagt tgtaaatgcc ataaaccgtt ctatggccat catcaccttc aatcctgaag
     1261 ggatcgtgct tgaagcaaat gaaaatttcg tcaatgccac tggctataag cgggatgaaa
     1321 tcataggtaa gcatcatcgt ctgttctgtg ccgagacgct ttacaaaagt gatgaatacc
     1381 gacatttctg ggagagtctg aatcaaggtg agtttttttc tggtctgttt ccacgcctta
     1441 accgtcaggg agaccctctg tggttccgtg caacttataa ccctgtcttt aacagtgatg
     1501 ggcagcttta taaaattgtg aaattcgcta cagatgttac ggctgatgtc ctgcgaaacc
     1561 agagagagca agaggctgct gtgcatgcat gggatatggc cgtgcagacc cgagaaagtg
     1621 cgcagaacgg tgccaatgtt attgaaaata gcatccttat gattgacaga attgcgcagg
     1681 ggatgggggc tgtctccacc gatatctcac ggctcaacaa tcagtctgaa agtattgacg
     1741 atatggtgga aaccatccgg aagtttgcca tgcagacaag acttattgca ctgaatgccg
     1801 cgattgaagc agcaagggct ggcgcatccg gaagaagttt tgcggttgtt gccgcagaag
     1861 tgcgtaatct tgcggcgagc gtcagtagcg cgactgaaga aattgagcaa gtcgtggcca
     1921 gcaatagtca actggccaag gatgttctct gtggcattga aaacagcctg atgaacactc
     1981 gggaaggggt tacactcatg cgcgaagcgg gtgaagttat gaccagcatt cagaggaatg
     2041 ctgcaggggt tgagactgcg gttaaggatg tcgccatttc agttaaagcc ggataggcca
     2101 tggtgcatat ccccatatct gtcgaacatc acagatgatc ttcttgagat cctttccgtt
     2161 gcgtcgtaat ctcttgctct gaaaacgaaa aaaccacccg gcaaggtggc tttttcgaag
     2221 gttcgcaaga gttgacgctc ttttgaaccg cggtaactgg cttggaggag cacagtcacc
     2281 aaaactgtcc tttcagtgta gccttatcgc acccgaaact tcaagactct cctcctctaa
     2341 acctgttacc agtggctgct gccagtggcg ctttgtcgtg tttttccgga ttggactcaa
     2401 gttgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
     2461 ccagcttgga gcgaactgcc ttcccggaac tgagtgtcag gcgtggaatg agacaaacgc
     2521 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2581 gagccaccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2641 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttc
     2701 gccgtggcct tatcgcttcc ctgttaagct tctttctggc atctcccagg aaatttccgc
     2761 cccgcctgta agccggtacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2821 gcgaggaagc ggaatatatc ctgtatcgca tattctgctg acgctccgct gctgcatttt
     2881 tctcctgcca catgaagcac ttcactgata ttcgcatccg tgtcaatata gtaagtcaat
     2941 atagtaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa
     3001 acccgccaaa agcctgacgc gcctgcggct tgtccaaccc cgcgccgacc gggaaaggct
     3061 ttcccgcccg tcccggcgtt atcaggaggc cggtttgtca gagaaacgga acaagatgct
     3121 caccatgtgg gtgacggagg acgagcaccg gcggctgctt gagcgctgtg acggcaggca
     3181 gctggcggcg tggatgcggc agacctgcct ggacgaaaaa cctgcgcgca ccggaaagct
     3241 cccctcaatc tcgccggcgc tgctgcgcca gcttgccggc atggggaata acctgaacca
     3301 gatagcccgc cgtgttaacg ccggcggcgg caccggccat gacagggtgc agattgtggc
     3361 ggcgctgatg gccatcgatg ccgggcttga gcggctgcgg cacgccgttc ttgagaaagg
     3421 aacggacgat gatcgttaaa tttcatccac gtggccgcgg cggtggcgcc gggccggtgg
     3481 actatctgct gggtaaagac cggcagcgtg aaggcgccag cgtcctgcag gggaagccgg
     3541 aagaagtccg ggagctgatt gacgcgtcgc cttacgtgaa gaaatacacc tccggcgtcc
     3601 tgtcgtttgc ggaggccgat ctgcctcccg gccagcggga aaaactgatg gccagcttcg
     3661 agcgggttct gatgcccgga cttgataaag atcagtacag cattctgtgg gtggagcacg
     3721 aggacaaggg gcgtctggag ctgaactttc tgatccccaa taccgagctg ctgaccggca
     3781 ggcggctcca gccgtactac gaccgcgccg accggccgcg tatcgatgcc tggcagacca
     3841 tcgtgaacgg cagactgggg ctgcacgatc cgaacgcgcc ggaaaaccgc cgggcgctgg
     3901 tcacgccggc cggactgccg aaaacgaagc aggaagccgc agaagccatc acccgtggac
     3961 tgctgagctt agcctcgtcc ggggagctga aaagccgtca ggacgtcact gaggctcttg
     4021 aaggtgcagg ttttgaggtg gtgcgtacaa cgaaaagcag tatcagcatt gccgaccctg
     4081 acggggggcg aaacatccga ctgaagggag ccatctatga gcagtctttt aacgctggcg
     4141 aaggacttag agcagcaatc gaaagcgcag cagaagagta ccggcgagat gctgaaagcc
     4201 gcattcagcg agcacgagca gtctgtcaga gcggaactga acgcaagcgc gaggagaatc
     4261 agcgacgcca tcagcgccca cgagcagagt atgagcgagg cgatggagaa aaaccggcgg
     4321 agcgtgttgc tgacggccgg gaggacatgg ctcaccatcc tgatggtgtc agcgctgctg
     4381 atcgccacga gcgggagcat tctgtggtgg caggggcagc agataaccga caattacacg
     4441 catcttcgtc agcaggaaga taccctggcg aaaatgaccg cccggacgtg gggagtgcgc
     4501 tatcaggaga gcagcgacgg ccggaggttt cttatcctgc cgccggggat gcaagccgaa
     4561 gccatcccct acgacgggac gacatggatc cgcctgaaac aggagtagcg gaaaatgaca
     4621 gagctggaaa agcagttgct gagcgtatta gagcagctac agcaggacta ctcgcaaagg
     4681 ctggacgaat gggagagcgc cttcgcggaa tggcggagca tgtgtggtct tatgcaacgg
     4741 gagaacgcgg tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct gagtttaagc
     4801 gagcagctgc gccgcttgtc cgggaactga atgccatcga ggagaaggct gcactggagt
     4861 atcagcgtca gaacgagctg gagctgacgc tccgacagaa gcagaagagt ttcgatggtc
     4921 cgaagatgag gtga
//
