LOCUS       NZ_CP049284             8379 bp    DNA     circular CON 27-SEP-2023
DEFINITION  Shigella boydii strain 600657 plasmid p600657_8, complete sequence.
ACCESSION   NZ_CP049284
VERSION     NZ_CP049284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14145980
            Assembly: GCF_013394535.1
KEYWORDS    RefSeq.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Hazen,T.H., Michalski,J.M. and Rasko,D.A.
  TITLE     Complete genome sequencing of Shigella boydii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Hazen,T.H., Michalski,J.M. and Rasko,D.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2020) Institute for Genome Sciences, University of
            Maryland, School of Medicine, 670 W. Baltimore St., Baltimore, MD
            21201, USA
  REMARK    Annotation added by submitter at the request of the submitter
REFERENCE   3  (bases 1 to 8379)
  AUTHORS   Hazen,T.H., Michalski,J.M. and Rasko,D.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2020) Institute for Genome Sciences, University of
            Maryland, School of Medicine, 670 W. Baltimore St., Baltimore, MD
            21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 27.896x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/27/2023 03:29:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,974
            Genes (coding)                    :: 4,128
            CDSs (with protein)               :: 4,128
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 846
            CDSs (without protein)            :: 846
            Pseudo Genes (ambiguous residues) :: 0 of 846
            Pseudo Genes (frameshifted)       :: 292 of 846
            Pseudo Genes (incomplete)         :: 549 of 846
            Pseudo Genes (internal stop)      :: 151 of 846
            Pseudo Genes (multiple problems)  :: 141 of 846
            Pseudo Genes (short protein)      :: 3 of 846
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049284.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /strain="600657"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /plasmid="p600657_8"
                     /geo_loc_name="Bangladesh"
                     /lat_lon="23.6850 N 90.3563 E"
                     /collected_by="University of Maryland, School of Medicine,
                     Center for Vaccine Development"
     gene            join(7987..8379,1..48)
                     /locus_tag="G5S55_RS25045"
                     /old_locus_tag="G5S55_25035"
     CDS             join(7987..8379,1..48)
                     /locus_tag="G5S55_RS25045"
                     /old_locus_tag="G5S55_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(793..942)
                     /locus_tag="G5S55_RS25050"
                     /old_locus_tag="G5S55_25040"
     CDS             complement(793..942)
                     /locus_tag="G5S55_RS25050"
                     /old_locus_tag="G5S55_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1513..1671
                     /locus_tag="G5S55_RS25950"
     CDS             1513..1671
                     /locus_tag="G5S55_RS25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2131..2946
                     /gene="sul2"
                     /locus_tag="G5S55_RS25055"
                     /old_locus_tag="G5S55_25045"
     ARG             2132..2943
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3007..3810
                     /gene="aph(3'')-Ib"
                     /locus_tag="G5S55_RS25060"
                     /old_locus_tag="G5S55_25050"
     ARG             3008..3807
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3810..4646
                     /locus_tag="G5S55_RS25065"
                     /old_locus_tag="G5S55_25055"
     CDS             3810..4646
                     /locus_tag="G5S55_RS25065"
                     /old_locus_tag="G5S55_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            4952..5194
                     /locus_tag="G5S55_RS25070"
                     /old_locus_tag="G5S55_25060"
     CDS             4952..5194
                     /locus_tag="G5S55_RS25070"
                     /old_locus_tag="G5S55_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5226..5876)
                     /gene="tetR(A)"
                     /locus_tag="G5S55_RS25075"
                     /old_locus_tag="G5S55_25065"
     CDS             complement(5226..5876)
                     /gene="tetR(A)"
                     /locus_tag="G5S55_RS25075"
                     /old_locus_tag="G5S55_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            5982..7181
                     /gene="tet(A)"
                     /locus_tag="G5S55_RS25080"
                     /old_locus_tag="G5S55_25070"
     ARG             5983..7178
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7148..7336)
                     /locus_tag="G5S55_RS25085"
                     /old_locus_tag="G5S55_25075"
                     /pseudo=""
     CDS             complement(<7148..7336)
                     /locus_tag="G5S55_RS25085"
                     /old_locus_tag="G5S55_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7380..7769)
                     /locus_tag="G5S55_RS25090"
                     /old_locus_tag="G5S55_25080"
     CDS             complement(7380..7769)
                     /locus_tag="G5S55_RS25090"
                     /old_locus_tag="G5S55_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             3811..4643
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(978..1151)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1075..1680
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaagcggg gattctggtc gcggctgttt cgctccggtt cagagtaaca gtgcaggtga
       61 ctctgattta ccataacatc tgttatcagc accgcctgag aggccctcag ggcgtttctg
      121 acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct
      181 ctgtttttac ggcatgcatg agtatgcgca caagtgcata gcgcggaaat tcacaccatt
      241 cagccggggt aagtcctgtt tccggcgttc ttcccatccg aaaacggtga acggggacgc
      301 aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaacccgct gtcagcacgt
      361 tcaggaaggg ggtgatgtac aaatggcaca gaggatgaaa gtgacgtatg agctgaagga
      421 atgatgtaaa agtgatgtat aagtgatgta aaagtgatgt atgaactgaa ggagcgatgt
      481 aattctgtag tcaggaagaa taacccgttc atgcaggcat gaacgataca gtgaggaacg
      541 ctttttttgt cacgatctcc ccttaacctg cgtaaatcac ccgccatggt tgattatttt
      601 tcaacctcaa aatggtcata cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat
      661 taaaagaaaa aaaaggaata tttcgttttc acgagtaata cccgggtatt aaaaatccac
      721 tgtaattaca tgaaaataaa gaaataaaag gtaataccgg agtattacca cgtacgcggg
      781 caagcgaaaa agctataacg cttcccggcc gacaggcgac ccaaaatcca caggggcatg
      841 caggttctct tcagtaacct gcgccattaa atcaaaaagg tcatattcgc cgggaagctg
      901 cgtatcagtc gaatccagtt gttcatcata ttcagatggc atggggtgat atccagtagc
      961 tgagaggttg ttagcgggtt tcggggcgca gccctgaacc agtcatgtag cgctagcgga
     1021 gtgtatactg gcttactatg ttgccacgat tgcgaaaatc agtgaagtgc ttcatgtggc
     1081 aggagaaaaa aggcagcacc ggcgcgtcag cagaatatgc gatacaggag atattccgct
     1141 tcctcgctca ctgactcgct acgctcggtc gttcggctgc ggcgagcggt accggcttac
     1201 ggacggggac ggaaatttcc tggaagatac caggaagaca cttagcaaag aagagataag
     1261 gccacggcga agccgttttt ccataggctc cgcccccctg acaagcatcg caaaatctga
     1321 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatcccaggc gtttccccct
     1381 ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     1441 ttatggccgc ggtttctcat tccacgcctg acactcagtt ccgtgaaggc agttcgctcc
     1501 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt gtccggtaac
     1561 tatcaacttg agtccaaccc ggaaagacac gacaaaacgc cactggcagc agccattagt
     1621 aacaggagtg cacgagaaga tagattaaga ggtcttgaag tagaggcctg actgcggcta
     1681 cactgaaaga acagttttgg taatggcggt ctcgtgaacc ctgttaccgg gttaagcagt
     1741 tccccaactg acttaacctt cgaacaaacc gcctccccag gcggtttttt cgtttacaga
     1801 gcaagagatt acgattcatt gaaaaggatc tcaagaagat cctttgtaga ttactatcaa
     1861 ttaatcttcc gaacatgaca ccagtcactg ttgattacgg ttaaaccgct ccgtaacagg
     1921 tttccctcgt attggtgact tgcagcacca aagaagcaaa aaagataaat atatctactt
     1981 attgagagat gatatattta tttccaaaat gctacagata catcatcatg aaacggatca
     2041 ccgcaaacag gttactcgcc tggggattcc ctttcgaccc gagcatccgt atgagactca
     2101 tgctcgatta ttattattat agaagccccc atgaataaat cgctcatcat tttcggcatc
     2161 gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc agacgcagcc
     2221 attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct cggtccggca
     2281 tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg
     2341 gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg
     2401 acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca
     2461 gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg
     2521 gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt
     2581 gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc
     2641 cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg
     2701 gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt
     2761 tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt cggggccgcg
     2821 acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg cacacacgag
     2881 ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt
     2941 cgttaactgc acattcggga tatttctcta tattcgcgct tcatcagaaa actgaaggaa
     3001 cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg gttgcctgtc
     3061 agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc cttcgcgaaa
     3121 atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct catttggctc
     3181 aaaggtcgag gtgtggcttg ccccgaggtc atcaactggc aggaggaaca ggagggtgca
     3241 tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg agcggatttg
     3301 ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct atcggttgat
     3361 caatgtccgt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga tgtggtgtcc
     3421 cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc gcagctcgat
     3481 cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg caccgatatg
     3541 gttgtttgcc atggtgatcc ctgcatgccg aacttcatgg tggaccctaa aactcttcaa
     3601 tgcacgggtc tgatcgacct tgggcggctc ggaacagcag atcgctatgc cgatttggca
     3661 ctcatgattg ctaacgccga agagaactgg gcagcgccag atgaagcaga gcgcgccttc
     3721 gctgtcctat tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct tgccttctat
     3781 ctgcgattgg accctctgac ttggggttga tgttcatgcc gcctgttttt cctgctcatt
     3841 ggcacgtttc gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt tggaaagttt
     3901 cattgccaga cgggactcct gcaatcgtca agggattgaa acctatagaa gacattgctg
     3961 atgaactgcg cggggccgac tatctggtat ggcgcaatgg gaggggagca gtccggttgc
     4021 tcggtcgtga gaacaatctg atgttgctcg aatatgccgg ggagcgaatg ctctctcaca
     4081 tcgttgccga gcacggcgac taccaggcga ccgaaattgc agcggaacta atggcgaagc
     4141 tgtatgccgc atctgaggaa cccctgcctt ctgcccttct cccgatccgg gatcgctttg
     4201 cagctttgtt tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc
     4261 acgcggcgat tatagccgat caaatgatga gcaatgcctc ggaactgcgt gggctacatg
     4321 gcgatctgca tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg atagatcccg
     4381 tcggtctggt cggtgaagtg ggctttggcg ccgccaatat gttctacgat ccggctgaca
     4441 gagacgacct ttgtctcgat cctagacgca ttgcacagat ggcggacgca ttctctcgtg
     4501 cgctggacgt cgatccgcgt cgcctgctcg accaggcgta cgcttatggg tgcctttccg
     4561 cagcttggaa cgcggatgga gaagaggagc aacgcgatct agctatcgcg gccgcgatca
     4621 agcaggtgcg acagacgtca tactagatat caagcgactt ctcctatccc ctgggaacac
     4681 atcaatctca ccggagaata tcgctggcca aagccttagc gtaggattcc gccccttccc
     4741 gcaaacgacc cctggaagcc ccgtagcgac gcggagaggg gcgagacaag ccaagggcgc
     4801 aggctcgatg cgcagcacga catagccggt tctcgcaagg acgagaattt ccctgcggtg
     4861 cccctcaagt gtcaatgaaa gtttccaacg cgagccattc gcgagagcct tgagtccacg
     4921 ctagatctat ctcatctgcg caaggcagaa cgtgaagacg gccgccctgg acctcgcccg
     4981 cgagcgccag gcgcacgagg ccggcgcgcg gacccgcgcc acggcccacg agcggacgcc
     5041 gcagcaggag cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg cttggacgct
     5101 agggcagggc atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc ggtggaaagg
     5161 gggaggggat gttgtctaca tggctctgct gtagtgagtg ggttgcgctc cggcagcggt
     5221 cctgatcaat cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag cctccttttc
     5281 gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg accggcttcg
     5341 tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc aacggtgccg
     5401 ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc aacagtgaag
     5461 tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc ctcgcagagg
     5521 aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt gccggcatgg
     5581 atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc attcccgatc
     5641 agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt ctccgccagc
     5701 atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc
     5761 ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc tacgccgacc
     5821 tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt tgtcatgctt
     5881 gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata aagaatccgc
     5941 gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc aacagacccc
     6001 tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg attatgccgg
     6061 tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc cactatggca
     6121 ttctgctggc gctgtatgcg ttgatgcaat ttgcctgcgc acctgtgctg ggcgcgctgt
     6181 cggatcgttt cgggcggcgg ccggtcttgc tcgtctcgct ggccggcgct gctgtcgact
     6241 acgccatcat ggcgacggcg cctttccttt gggttctcta tatcgggcgg atcgtggccg
     6301 gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc actgatggcg
     6361 atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg atggtcgcgg
     6421 gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc ttcgccgcgg
     6481 cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag tcgcacaaag
     6541 gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc cggtgggccc
     6601 ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa cttgtcggac
     6661 aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg gacgcgacca
     6721 cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag gcaatgatca
     6781 ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga atgattgccg
     6841 acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg ttcccgatca
     6901 tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc
     6961 aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc accagcctga
     7021 cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata acaacgtgga
     7081 acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc
     7141 gcgggctttg gagaaattct tcaaattccc gttgcacata gcccggcaat tcctttccct
     7201 gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg
     7261 tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg
     7321 ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct
     7381 tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca
     7441 gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt attgcgtgca
     7501 ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt aatattcttc
     7561 gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc agtataagat
     7621 gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc tcccgaactt
     7681 ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc aaaaccaccg
     7741 acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt taaaaacagc
     7801 aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac gcccgtcaaa
     7861 accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc ctcagacctt
     7921 ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg agacactgtc
     7981 acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa gtcgcgctcg
     8041 caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa aaatgatcgc
     8101 cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac ttctgagaca
     8161 ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga aaatgctcag
     8221 ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct aatgcttgaa
     8281 gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga acaactacag
     8341 gccgaaatta gccgtctggc tgacgcgctg gcacaggaa
//
